GZMH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GZMH
Entrez Gene ID	2999
Full Name	granzyme H
Synonyms	CCP-X,CGL-2,CSP-C,CTLA1,CTSGL2
General protein information	Preferred Names granzyme H Names granzyme H cathepsin G-like 2, protein h-CCPX cytotoxic T-lymphocyte proteinase cytotoxic T-lymphocyte-associated serine esterase 1 cytotoxin serine protease-C
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q12
Summary	This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015].
Disorder MIM:	116831

mRNA and Protein(s)

mRNA	Protein	Name
NM_033423.5	NP_219491.1	granzyme H isoform 1 preproprotein
NM_001270781.2	NP_001257710.1	granzyme H isoform 3 precursor
NM_001270780.2	NP_001257709.1	granzyme H isoform 2 precursor
XM_011536683.2	XP_011534985.1	granzyme H isoform X1
NM_033423.4	NP_219491.1	granzyme H isoform 1 preproprotein
NM_001270780.1	NP_001257709.1	granzyme H isoform 2 precursor
NM_001270781.1	NP_001257710.1	granzyme H isoform 3 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GZMH	NP_219491.1
Pan troglodytes (chimpanzee)	GZMH	XP_522811.2
Mus musculus (house mouse)	Gzmd	NP_034502.2
Mus musculus (house mouse)	Gzme	NP_034503.2
Mus musculus (house mouse)	Gzmg	NP_034505.1
Rattus norvegicus (Norway rat)	Gzmf	NP_703196.1
Mus musculus (house mouse)	Gzmf	NP_034504.1

Gene Ontology
Bibliography

Related articles in PubMed

Granzyme h serum levels variations with both reproductive hormone receptors, and related hormone receptors in breast cancer patients.
Tahbaz-Lahafi B, Akbari ME, Amir-Rassouli H, Rahimipour A, Azargashb E, Nafissi N, Jahani F
Iranian journal of cancer prevention7(1)48-52(2014)

Granzyme H is a novel protease expressed by human mast cells.
Rönnberg E, Calounova G, Sutton VR, Trapani JA, Rollman O, Hagforsen E, Pejler G
International archives of allergy and immunology165(1)68-74(2014)

Granzyme H induces cell death primarily via a Bcl-2-sensitive mitochondrial cell death pathway that does not require direct Bid activation.
Ewen CL, Kane KP, Bleackley RC
Molecular immunology54(3-4)309-18(2013 Jul)

Structural insights into the substrate specificity of human granzyme H: the functional roles of a novel RKR motif.
Wang L, Zhang K, Wu L, Liu S, Zhang H, Zhou Q, Tong L, Sun F, Fan Z
Journal of immunology (Baltimore, Md. : 1950)188(2)765-73(2012 Jan)

Cleavage of La protein by granzyme H induces cytoplasmic translocation and interferes with La-mediated HCV-IRES translational activity.
Romero V, Fellows E, Jenne DE, Andrade F
Cell death and differentiation16(2)340-8(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GZMH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GZMH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12486	NM_001270780.2 Latest version!	Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu12963	NM_001270781.2 Latest version!	Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu58173	XM_011536683.2 Latest version!	Homo sapiens granzyme H (GZMH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu12576	NM_033423.5 Latest version!	Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu12576	NM_033423.4	Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu12486	NM_001270780.1	Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu12963	NM_001270781.1	Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GZMH ( NM_001270780.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257709.1 Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12486
Clone ID Related Accession (Same CDS sequence)	NM_001270780.2 , NM_001270780.1
Accession Version	NM_001270780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	granzyme H isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ054303.1, CD000418.1 and AW204493.1. On May 31, 2019 this sequence version replaced NM_001270780.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD000418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGGGGA CTCCGGGGGG 
CCCCTCGTGT GTAAGGACGT AGCCCAAGGT ATTCTCTCCT ATGGAAACAA AAAAGGGACA 
CCTCCAGGAG TCTACATCAA GGTCTCACAC TTCCTGCCCT GGATAAAGAG AACAATGAAG 
CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257709.1
CDS	73..621
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_001270780.2
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270780.2

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGGGGA CTCCGGGGGG 
CCCCTCGTGT GTAAGGACGT AGCCCAAGGT ATTCTCTCCT ATGGAAACAA AAAAGGGACA 
CCTCCAGGAG TCTACATCAA GGTCTCACAC TTCCTGCCCT GGATAAAGAG AACAATGAAG 
CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( NM_001270780.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257709.1 Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12486
Clone ID Related Accession (Same CDS sequence)	NM_001270780.2 , NM_001270780.1
Accession Version	NM_001270780.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529769600000
Organism	Homo sapiens(human)
Product	granzyme H isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ054303.1, AL136018.4, CD000418.1 and AW204493.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD000418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGGGGA CTCCGGGGGG 
CCCCTCGTGT GTAAGGACGT AGCCCAAGGT ATTCTCTCCT ATGGAAACAA AAAAGGGACA 
CCTCCAGGAG TCTACATCAA GGTCTCACAC TTCCTGCCCT GGATAAAGAG AACAATGAAG 
CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257709.1
CDS	108..656
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_001270780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270780.1

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGGGGA CTCCGGGGGG 
CCCCTCGTGT GTAAGGACGT AGCCCAAGGT ATTCTCTCCT ATGGAAACAA AAAAGGGACA 
CCTCCAGGAG TCTACATCAA GGTCTCACAC TTCCTGCCCT GGATAAAGAG AACAATGAAG 
CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( NM_001270781.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257710.1 Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12963
Clone ID Related Accession (Same CDS sequence)	NM_001270781.2 , NM_001270781.1
Accession Version	NM_001270781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	granzyme H isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY232657.1 and AW204493.1. On May 31, 2019 this sequence version replaced NM_001270781.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY232657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGG GGGACTCCGG GGGGCCCCTC 
GTGTGTAAGG ACGTAGCCCA AGGTATTCTC TCCTATGGAA ACAAAAAAGG GACACCTCCA 
GGAGTCTACA TCAAGGTCTC ACACTTCCTG CCCTGGATAA AGAGAACAAT GAAGCGCCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257710.1
CDS	73..555
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_001270781.2
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270781.2

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGG GGGACTCCGG GGGGCCCCTC 
GTGTGTAAGG ACGTAGCCCA AGGTATTCTC TCCTATGGAA ACAAAAAAGG GACACCTCCA 
GGAGTCTACA TCAAGGTCTC ACACTTCCTG CCCTGGATAA AGAGAACAAT GAAGCGCCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( NM_001270781.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257710.1 Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12963
Clone ID Related Accession (Same CDS sequence)	NM_001270781.2 , NM_001270781.1
Accession Version	NM_001270781.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	granzyme H isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ054303.1, AL136018.4, AY232657.1 and AW204493.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. This isoform (3) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY232657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGG GGGACTCCGG GGGGCCCCTC 
GTGTGTAAGG ACGTAGCCCA AGGTATTCTC TCCTATGGAA ACAAAAAAGG GACACCTCCA 
GGAGTCTACA TCAAGGTCTC ACACTTCCTG CCCTGGATAA AGAGAACAAT GAAGCGCCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257710.1
CDS	108..590
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_001270781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270781.1

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGG GGGACTCCGG GGGGCCCCTC 
GTGTGTAAGG ACGTAGCCCA AGGTATTCTC TCCTATGGAA ACAAAAAAGG GACACCTCCA 
GGAGTCTACA TCAAGGTCTC ACACTTCCTG CCCTGGATAA AGAGAACAAT GAAGCGCCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( XM_011536683.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534985.1 Homo sapiens granzyme H (GZMH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58173
Clone ID Related Accession (Same CDS sequence)	XM_011536683.2
Accession Version	XM_011536683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	granzyme H isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG TTCAGTTTCT GCAAGAGAAG AGTCGGAAGA GGTGTGGCGG CATCCTAGTG 
AGAAAGGACT TTGTGCTGAC AGCTGCTCAC TGCCAGGGAA GCTCCATAAA TGTCACCTTG 
GGGGCCCACA ATATCAAGGA ACAGGAGCGG ACCCAGCAGT TTATCCCTGT GAAAAGACCC 
ATCCCCCATC CAGCCTATAA TCCTAAGAAC TTCTCCAACG ACATCATGCT ACTGCAGCTG 
GAGAGAAAGG CCAAGTGGAC CACAGCTGTG CGGCCTCTCA GGCTACCTAG CAGCAAGGCC 
CAGGTGAAGC CAGGGCAGCT GTGCAGTGTG GCTGGCTGGG GTTATGTCTC AATGAGCACT 
TTAGCAACCA CACTGCAGGA AGTGTTGCTG ACAGTGCAGA AGGACTGCCA GTGTGAACGT 
CTCTTCCATG GCAATTACAG CAGAGCCACT GAGATTTGTG TGGGGGATCC AAAGAAGACA 
CAGACCGGTT TCAAGGGGGA CTCCGGGGGG CCCCTCGTGT GTAAGGACGT AGCCCAAGGT 
ATTCTCTCCT ATGGAAACAA AAAAGGGACA CCTCCAGGAG TCTACATCAA GGTCTCACAC 
TTCCTGCCCT GGATAAAGAG AACAATGAAG CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534985.1
CDS	266..904
Translation	MAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQVKPGQLCSVAGWGYVSMSTLATTLQEVLLTVQKDCQCERLFHGNYSRATEICVGDPKKTQTGFKGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	XM_011536683.2
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536683.2

ORF Insert Sequence:

ATGGCCTTTG TTCAGTTTCT GCAAGAGAAG AGTCGGAAGA GGTGTGGCGG CATCCTAGTG 
AGAAAGGACT TTGTGCTGAC AGCTGCTCAC TGCCAGGGAA GCTCCATAAA TGTCACCTTG 
GGGGCCCACA ATATCAAGGA ACAGGAGCGG ACCCAGCAGT TTATCCCTGT GAAAAGACCC 
ATCCCCCATC CAGCCTATAA TCCTAAGAAC TTCTCCAACG ACATCATGCT ACTGCAGCTG 
GAGAGAAAGG CCAAGTGGAC CACAGCTGTG CGGCCTCTCA GGCTACCTAG CAGCAAGGCC 
CAGGTGAAGC CAGGGCAGCT GTGCAGTGTG GCTGGCTGGG GTTATGTCTC AATGAGCACT 
TTAGCAACCA CACTGCAGGA AGTGTTGCTG ACAGTGCAGA AGGACTGCCA GTGTGAACGT 
CTCTTCCATG GCAATTACAG CAGAGCCACT GAGATTTGTG TGGGGGATCC AAAGAAGACA 
CAGACCGGTT TCAAGGGGGA CTCCGGGGGG CCCCTCGTGT GTAAGGACGT AGCCCAAGGT 
ATTCTCTCCT ATGGAAACAA AAAAGGGACA CCTCCAGGAG TCTACATCAA GGTCTCACAC 
TTCCTGCCCT GGATAAAGAG AACAATGAAG CGCCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( NM_033423.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_219491.1 Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.

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CloneID	OHu12576
Clone ID Related Accession (Same CDS sequence)	NM_033423.5 , NM_033423.4
Accession Version	NM_033423.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	granzyme H isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ054303.1, BC027974.1 and AW204493.1. On Nov 24, 2018 this sequence version replaced NM_033423.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ054303.1, SRR5189664.11848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216338.9/ ENSP00000216338.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGTGAA GCCAGGGCAG 
CTGTGCAGTG TGGCTGGCTG GGGTTATGTC TCAATGAGCA CTTTAGCAAC CACACTGCAG 
GAAGTGTTGC TGACAGTGCA GAAGGACTGC CAGTGTGAAC GTCTCTTCCA TGGCAATTAC 
AGCAGAGCCA CTGAGATTTG TGTGGGGGAT CCAAAGAAGA CACAGACCGG TTTCAAGGGG 
GACTCCGGGG GGCCCCTCGT GTGTAAGGAC GTAGCCCAAG GTATTCTCTC CTATGGAAAC 
AAAAAAGGGA CACCTCCAGG AGTCTACATC AAGGTCTCAC ACTTCCTGCC CTGGATAAAG 
AGAACAATGA AGCGCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_219491.1
CDS	73..813
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQVKPGQLCSVAGWGYVSMSTLATTLQEVLLTVQKDCQCERLFHGNYSRATEICVGDPKKTQTGFKGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_033423.5
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033423.5

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGTGAA GCCAGGGCAG 
CTGTGCAGTG TGGCTGGCTG GGGTTATGTC TCAATGAGCA CTTTAGCAAC CACACTGCAG 
GAAGTGTTGC TGACAGTGCA GAAGGACTGC CAGTGTGAAC GTCTCTTCCA TGGCAATTAC 
AGCAGAGCCA CTGAGATTTG TGTGGGGGAT CCAAAGAAGA CACAGACCGG TTTCAAGGGG 
GACTCCGGGG GGCCCCTCGT GTGTAAGGAC GTAGCCCAAG GTATTCTCTC CTATGGAAAC 
AAAAAAGGGA CACCTCCAGG AGTCTACATC AAGGTCTCAC ACTTCCTGCC CTGGATAAAG 
AGAACAATGA AGCGCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GZMH ( NM_033423.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_219491.1 Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12576
Clone ID Related Accession (Same CDS sequence)	NM_033423.5 , NM_033423.4
Accession Version	NM_033423.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	granzyme H isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ054303.1, AL136018.4, BC027974.1 and AW204493.1. On Aug 1, 2012 this sequence version replaced NM_033423.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ054303.1, BC027974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGTGAA GCCAGGGCAG 
CTGTGCAGTG TGGCTGGCTG GGGTTATGTC TCAATGAGCA CTTTAGCAAC CACACTGCAG 
GAAGTGTTGC TGACAGTGCA GAAGGACTGC CAGTGTGAAC GTCTCTTCCA TGGCAATTAC 
AGCAGAGCCA CTGAGATTTG TGTGGGGGAT CCAAAGAAGA CACAGACCGG TTTCAAGGGG 
GACTCCGGGG GGCCCCTCGT GTGTAAGGAC GTAGCCCAAG GTATTCTCTC CTATGGAAAC 
AAAAAAGGGA CACCTCCAGG AGTCTACATC AAGGTCTCAC ACTTCCTGCC CTGGATAAAG 
AGAACAATGA AGCGCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_219491.1
CDS	108..848
Translation	MQPFLLLLAFLLTPGAGTEEIIGGHEAKPHSRPYMAFVQFLQEKSRKRCGGILVRKDFVLTAAHCQGSSINVTLGAHNIKEQERTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQVKPGQLCSVAGWGYVSMSTLATTLQEVLLTVQKDCQCERLFHGNYSRATEICVGDPKKTQTGFKGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTMKRL

Target ORF information:

RefSeq Version	NM_033423.4
Organism	Homo sapiens(human)
Definition	Homo sapiens granzyme H (GZMH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033423.4

ORF Insert Sequence:

ATGCAGCCAT TCCTCCTCCT GTTGGCCTTT CTTCTGACCC CTGGGGCTGG GACAGAGGAG 
ATCATCGGGG GCCATGAGGC CAAGCCCCAC TCCCGCCCCT ACATGGCCTT TGTTCAGTTT 
CTGCAAGAGA AGAGTCGGAA GAGGTGTGGC GGCATCCTAG TGAGAAAGGA CTTTGTGCTG 
ACAGCTGCTC ACTGCCAGGG AAGCTCCATA AATGTCACCT TGGGGGCCCA CAATATCAAG 
GAACAGGAGC GGACCCAGCA GTTTATCCCT GTGAAAAGAC CCATCCCCCA TCCAGCCTAT 
AATCCTAAGA ACTTCTCCAA CGACATCATG CTACTGCAGC TGGAGAGAAA GGCCAAGTGG 
ACCACAGCTG TGCGGCCTCT CAGGCTACCT AGCAGCAAGG CCCAGGTGAA GCCAGGGCAG 
CTGTGCAGTG TGGCTGGCTG GGGTTATGTC TCAATGAGCA CTTTAGCAAC CACACTGCAG 
GAAGTGTTGC TGACAGTGCA GAAGGACTGC CAGTGTGAAC GTCTCTTCCA TGGCAATTAC 
AGCAGAGCCA CTGAGATTTG TGTGGGGGAT CCAAAGAAGA CACAGACCGG TTTCAAGGGG 
GACTCCGGGG GGCCCCTCGT GTGTAAGGAC GTAGCCCAAG GTATTCTCTC CTATGGAAAC 
AAAAAAGGGA CACCTCCAGG AGTCTACATC AAGGTCTCAC ACTTCCTGCC CTGGATAAAG 
AGAACAATGA AGCGCCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Granzyme h serum levels variations with both reproductive hormone receptors, and related hormone receptors in breast cancer patients.
ian journal of cancer prevention7(1)48-52(2014)
Tahbaz-Lahafi B,Akbari ME,Amir-Rassouli H,Rahimipour A,Azargashb E,Nafissi N,Jahani F

Granzyme H is a novel protease expressed by human mast cells.
International archives of allergy and immunology165(1)68-74(2014)
Rönnberg E,Calounova G,Sutton VR,Trapani JA,Rollman O,Hagforsen E,Pejler G

Granzyme H induces cell death primarily via a Bcl-2-sensitive mitochondrial cell death pathway that does not require direct Bid activation.
Molecular immunology54(3-4)309-18(2013 Jul)
Ewen CL,Kane KP,Bleackley RC

Structural insights into the substrate specificity of human granzyme H: the functional roles of a novel RKR motif.
Journal of immunology (Baltimore, Md. : 1950)188(2)765-73(2012 Jan)
Wang L,Zhang K,Wu L,Liu S,Zhang H,Zhou Q,Tong L,Sun F,Fan Z

Cleavage of La protein by granzyme H induces cytoplasmic translocation and interferes with La-mediated HCV-IRES translational activity.
Cell death and differentiation16(2)340-8(2009 Feb)
Romero V,Fellows E,Jenne DE,Andrade F

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GZMH cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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