NLRP3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP3
Entrez Gene ID	423021
Full Name	NLR family pyrin domain containing-like
Synonyms	CIAS1,NLRP3,Nalp3
General protein information	Preferred Names NLR family pyrin domain containing-like Names NACHT, LRR and PYD domains-containing protein 3 NACHT, LRR and PYD domains-containing protein 12 NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 12 NLR family pyrin domain containing 3 cold autoinflammatory syndrome 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025150665.1	XP_025006433.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
NM_001348947.1	NP_001335876.1	NACHT, LRR and PYD domains-containing protein 3
XM_015286283.1	XP_015141769.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NLRP3	NP_004886.3
Macaca mulatta (Rhesus monkey)	NLRP3	NP_001107823.1
Xenopus tropicalis (tropical clawed frog)	LOC101730849	XP_004917660.1
Xenopus tropicalis (tropical clawed frog)	LOC100487412	XP_002938247.2
Xenopus tropicalis (tropical clawed frog)	LOC100488807	XP_002943171.1
Rattus norvegicus (Norway rat)	Nlrp3	NP_001178571.1
Xenopus tropicalis (tropical clawed frog)	LOC101731251	XP_004911151.1
Xenopus tropicalis (tropical clawed frog)	LOC100489137	XP_002943173.1
Canis lupus familiaris (dog)	NLRP3	XP_848377.2
Mus musculus (house mouse)	Nlrp3	NP_665826.1
Xenopus tropicalis (tropical clawed frog)	LOC101730480	XP_004917655.1
Xenopus tropicalis (tropical clawed frog)	LOC100488969	XP_002943172.2
Pan troglodytes (chimpanzee)	NLRP3	XP_003949788.1
Xenopus tropicalis (tropical clawed frog)	LOC101730955	XP_004917628.1
Xenopus tropicalis (tropical clawed frog)	LOC101730774	XP_004917659.1
Xenopus tropicalis (tropical clawed frog)	LOC100486737	XP_002937318.2
Xenopus tropicalis (tropical clawed frog)	LOC100491706	XP_002944439.2
Xenopus tropicalis (tropical clawed frog)	LOC100495083	XP_002940532.2
Bos taurus (cattle)	NLRP3	NP_001095689.1
Gallus gallus (chicken)	NLRP3	XP_001233262.3
Xenopus tropicalis (tropical clawed frog)	LOC100486578	XP_002937317.2
Xenopus tropicalis (tropical clawed frog)	LOC100488643	XP_002943170.2
Xenopus tropicalis (tropical clawed frog)	LOC100493406	XP_004917630.1
Xenopus tropicalis (tropical clawed frog)	LOC100497524	XP_002945565.2

Gene Ontology
Bibliography

Related articles in PubMed

Tissue-specific expression pattern and histological distribution of NLRP3 in Chinese yellow chicken.
Ye J, Yu M, Zhang K, Liu J, Wang Q, Tao P, Jia K, Liao M, Ning Z
Veterinary research communications39(3)171-7(2015 Sep)

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa36096	NM_001348947.1 Latest version!	Gallus gallus NLR family pyrin domain containing-like (NLRPL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa150818	XM_025150665.1 Latest version!	Gallus gallus NLR family pyrin domain containing-like (NLRPL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa36096	XM_015286283.1 Latest version!	Gallus gallus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP3 ( NM_001348947.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001335876.1 Gallus gallus NLR family pyrin domain containing-like (NLRPL), mRNA.

Price & Availability↑

CloneID	OGa36096
Clone ID Related Accession (Same CDS sequence)	NM_001348947.1 , XM_015286283.1
Accession Version	NM_001348947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498492800000
Organism	Gallus gallus(chicken)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000190.1. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03354477, SAMN03354484 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTG CTGCGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTCCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT ACTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335876.1
CDS	21..2798
Translation	MAGEESTILLEALEGLTLEDFQEFKKKLPHTDIKGGWNIGRDELEKVTHPSSLISYMGDSYGEGAAMDIAISLFEEMNQRDLAEKILDEKVKEYKQKYTEHVAREFLQYKEANSCLGENLSVRDRYTNLTIARKSWDQHGDEPGDVSSDTVTTQTLLEPSKDGQVPPITVLVGASGMGKTMTIRKVMMEWVEGTLCTQFDYVFCIDCKELSFSKEVSMVDLISKCCPQQRMPAGRILGNPEKILFIFDSFEALGLPLAQPKDELSTDPTEAKPLETTLLSLLRRTVLPESSVLIATRPAALQSLGQCLEGKHYVEILGFSPAAREEYFHRYFGNDSKADVAFRFTRGNEVLYSLCVIPVMSWTVCTVLERELYERNQLLACSKTTTQMIMFYLSWLMKHRVSNAWQNLQQFLHKLCSLAADGIWKHKVLFEEKEIEDQGLNQPQLLSLFLNEKGLEKGTDHVNVYSFSHLHLQELFAAMFYVLEDQDGMVSDSRILAKDVNMLLESYHTSRMDLNVTVRLLFGLVNPKSVEYAGEGIGCRISLQPQEDLLRWLQTRPRGTSHPREVMKIEDLDTFHLLFETNEKSFVQSVLGSFTGIALQDVKLTLYDQAALCFCIKQWAGLLSVTLRSCSFHQQHHRQEPAKGLPRQSWRQEELHSPLHPLCQALGHPGSSLQSLRLQWCGLTEGDSGALGMLLATLPSLVHLELGDGALGDDGVRMLCAGLRQPGCQLRVLRLRYTHLTSACCQDLAVALGTSTCLEELDLSFSAGLRDDGVKLLCEGLQHSGCQLRVLRLGSCSLTGACCQALVAWLGHSHGLKCLDLSDTELGAGATLLLQHLRHHSCTLQTLGLSTSTLSKDALQELAALRALKPSLKITDLLEHEAPETGAMARLTFQRSVWAGRGAAVRGRKGLPSSRAAPPHSRSLC

Target ORF information:

RefSeq Version	NM_001348947.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family pyrin domain containing-like (NLRPL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348947.1

ORF Insert Sequence:

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTG CTGCGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTCCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT ACTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_025150665.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006433.1 Gallus gallus NLR family pyrin domain containing-like (NLRPL), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa150818
Clone ID Related Accession (Same CDS sequence)	XM_025150665.1
Accession Version	XM_025150665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTC TGCAGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTGCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT GCTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006433.1
CDS	4136..6913
Translation	MAGEESTILLEALEGLTLEDFQEFKKKLPHTDIKGGWNIGRDELEKVTHPSSLISYMGDSYGEGAAMDIAISLFEEMNQRDLAEKILDEKVKEYKQKYTEHVAREFLQYKEANSCLGENLSVRDRYTNLTIARKSWDQHGDEPGDVSSDTVTTQTLLEPSKDGQVPPITVLVGASGMGKTMTIRKVMMEWVEGTLCTQFDYVFCIDCKELSFSKEVSMVDLISKCCPQQRMPAGRILGNPEKILFIFDSFEALGLPLAQPKDELSTDPTEAKPLETTLLSLLRRTVLPESSVLIATRPAALQSLGQCLEGKHYVEILGFSPAAREEYFHRYFGNDSKADVAFRFTRGNEVLYSLCVIPVMSWTVCTVLERELYERNQLLACSKTTTQMIMFYLSWLMKHRVSNAWQNLQQFLHKLCSLAADGIWKHKVLFEEKEIEDQGLNQPQLLSLFLNEKGLEKGTDHVNVYSFSHLHLQELFAAMFYVLEDQDGMVSDSRILAKDVNMLLESYHTSRMDLNVTVRLLFGLVNPKSVEYAGEGIGCRISLQPQEDLLRWLQTRPRGTSHPREVMKIEDLDTFHLLFETNEKSFVQSVLGSFTGIALQDVKLTLYDQAALCFCIKQWAGLLSVTLRSCSFHQQHHRQEPAKGLPRQSWRQEELHSPLHPLCQALGHPGSSLQSLRLQWCGLTEGDSGALGMLLATLPSLVHLELGDGALGDDGVRMLCAGLRQPGCQLRVLRLRYTHLTSACCQDLAVALGTSTCLEELDLSFSAGLRDDGVKLLCEGLQHSGCQLRVCRLGSCSLTGACCQALVAWLGHSHGLKCLDLSDTELGAGATLLLQHLRHHSCTLQTLGLSTSTLSKDALQELAALRALKPSLKITDLLEHEAPETGAMARLTFQRSVWAGRGAAVRGRKGLPSSRAAPPHSRSLC

Target ORF information:

RefSeq Version	XM_025150665.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family pyrin domain containing-like (NLRPL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150665.1

ORF Insert Sequence:

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTC TGCAGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTGCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT GCTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_015286283.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141769.1 Gallus gallus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

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CloneID	OGa36096
Clone ID Related Accession (Same CDS sequence)	NM_001348947.1 , XM_015286283.1
Accession Version	XM_015286283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTG CTGCGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTCCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT ACTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141769.1
CDS	1621..4398
Translation	MAGEESTILLEALEGLTLEDFQEFKKKLPHTDIKGGWNIGRDELEKVTHPSSLISYMGDSYGEGAAMDIAISLFEEMNQRDLAEKILDEKVKEYKQKYTEHVAREFLQYKEANSCLGENLSVRDRYTNLTIARKSWDQHGDEPGDVSSDTVTTQTLLEPSKDGQVPPITVLVGASGMGKTMTIRKVMMEWVEGTLCTQFDYVFCIDCKELSFSKEVSMVDLISKCCPQQRMPAGRILGNPEKILFIFDSFEALGLPLAQPKDELSTDPTEAKPLETTLLSLLRRTVLPESSVLIATRPAALQSLGQCLEGKHYVEILGFSPAAREEYFHRYFGNDSKADVAFRFTRGNEVLYSLCVIPVMSWTVCTVLERELYERNQLLACSKTTTQMIMFYLSWLMKHRVSNAWQNLQQFLHKLCSLAADGIWKHKVLFEEKEIEDQGLNQPQLLSLFLNEKGLEKGTDHVNVYSFSHLHLQELFAAMFYVLEDQDGMVSDSRILAKDVNMLLESYHTSRMDLNVTVRLLFGLVNPKSVEYAGEGIGCRISLQPQEDLLRWLQTRPRGTSHPREVMKIEDLDTFHLLFETNEKSFVQSVLGSFTGIALQDVKLTLYDQAALCFCIKQWAGLLSVTLRSCSFHQQHHRQEPAKGLPRQSWRQEELHSPLHPLCQALGHPGSSLQSLRLQWCGLTEGDSGALGMLLATLPSLVHLELGDGALGDDGVRMLCAGLRQPGCQLRVLRLRYTHLTSACCQDLAVALGTSTCLEELDLSFSAGLRDDGVKLLCEGLQHSGCQLRVLRLGSCSLTGACCQALVAWLGHSHGLKCLDLSDTELGAGATLLLQHLRHHSCTLQTLGLSTSTLSKDALQELAALRALKPSLKITDLLEHEAPETGAMARLTFQRSVWAGRGAAVRGRKGLPSSRAAPPHSRSLC

Target ORF information:

RefSeq Version	XM_015286283.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NLR family, pyrin domain containing 3 (NLRP3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286283.1

ORF Insert Sequence:

ATGGCAGGAG AAGAAAGCAC CATCCTCTTA GAAGCACTGG AAGGTCTCAC ACTCGAAGAC 
TTCCAAGAGT TCAAGAAGAA GCTGCCACAC ACTGATATCA AAGGAGGCTG GAACATCGGC 
AGGGATGAGC TGGAGAAGGT CACCCATCCT TCCTCACTCA TCAGTTATAT GGGCGACAGC 
TATGGGGAAG GTGCTGCTAT GGACATTGCC ATCAGTCTGT TTGAGGAGAT GAACCAGAGG 
GACCTTGCTG AAAAAATCCT TGATGAGAAG GTGAAAGAGT ACAAGCAGAA ATACACAGAG 
CACGTGGCCA GAGAGTTCCT GCAGTACAAA GAGGCGAATT CCTGCCTAGG GGAGAACCTG 
TCTGTGCGCG ACCGCTACAC CAACCTGACC ATAGCCAGGA AGTCTTGGGA TCAGCACGGA 
GATGAGCCTG GAGATGTCAG CTCTGACACT GTCACCACAC AAACACTCCT TGAACCCAGC 
AAAGACGGCC AGGTGCCGCC GATCACGGTG CTGGTGGGTG CCTCTGGCAT GGGGAAGACG 
ATGACGATCA GGAAGGTGAT GATGGAGTGG GTGGAAGGGA CCCTCTGCAC ACAGTTTGAC 
TATGTCTTCT GCATAGACTG CAAGGAGCTT TCCTTTTCCA AAGAAGTGAG CATGGTGGAC 
CTGATTTCAA AATGCTGCCC TCAACAGCGA ATGCCGGCTG GAAGGATTTT GGGTAACCCG 
GAGAAGATCC TGTTCATCTT TGATAGCTTT GAGGCCCTGG GATTGCCCTT GGCTCAGCCG 
AAGGACGAGC TGAGCACTGA CCCCACGGAA GCCAAACCAC TGGAAACCAC CCTGCTGAGC 
TTGCTCAGGA GAACAGTCCT CCCTGAGTCC TCTGTGCTCA TTGCCACGAG ACCAGCAGCT 
CTCCAGAGCC TGGGGCAGTG CCTGGAGGGC AAGCATTATG TGGAAATCCT GGGGTTTTCA 
CCAGCTGCAA GGGAGGAGTA TTTCCACAGG TATTTTGGGA ATGACAGTAA AGCAGATGTG 
GCCTTCAGGT TTACCAGGGG AAATGAGGTC CTCTACAGCT TGTGTGTCAT CCCTGTCATG 
AGCTGGACTG TCTGCACCGT CCTTGAGCGA GAGCTGTACG AGAGGAATCA GCTCCTTGCG 
TGCTCTAAGA CCACCACACA GATGATCATG TTCTACCTCT CCTGGTTAAT GAAGCATAGA 
GTCAGCAATG CCTGGCAGAA CCTGCAGCAG TTCCTGCACA AGCTTTGCTC CTTGGCTGCT 
GACGGCATCT GGAAGCACAA GGTCCTCTTT GAGGAGAAGG AAATTGAGGA CCAAGGCCTG 
AATCAGCCAC AGCTCCTCTC CCTCTTCCTC AATGAGAAAG GTTTGGAGAA AGGCACAGAC 
CATGTGAATG TCTACAGCTT CAGCCACCTG CACCTCCAGG AGCTCTTCGC AGCGATGTTT 
TACGTCCTGG AGGACCAGGA CGGAATGGTC AGTGACTCAC GGATCCTTGC AAAGGACGTG 
AATATGTTGT TAGAAAGCTA CCACACATCT AGGATGGATT TAAACGTCAC AGTGAGGCTC 
CTGTTTGGTC TGGTGAACCC CAAGTCAGTA GAGTACGCGG GTGAAGGAAT CGGGTGCAGA 
ATTTCATTGC AACCTCAGGA AGATCTGCTG AGGTGGCTTC AGACACGGCC CAGAGGCACT 
TCCCATCCCC GCGAAGTGAT GAAGATTGAG GATTTGGACA CCTTCCACCT TTTATTCGAG 
ACAAACGAAA AAAGCTTTGT GCAGAGCGTG TTGGGCAGTT TCACAGGAAT AGCCTTGCAG 
GACGTCAAGC TGACACTGTA TGACCAGGCG GCTCTTTGCT TCTGCATCAA GCAGTGGGCC 
GGGCTGCTCA GTGTCACCCT CCGGAGCTGC TCTTTCCACC AGCAGCATCA CAGGCAGGAG 
CCAGCCAAGG GCTTGCCCCG GCAGAGCTGG CGGCAGGAGG AGCTGCACTC ACCTCTGCAC 
CCGCTGTGCC AGGCCCTGGG GCACCCAGGC AGCTCACTCC AGAGCCTCAG GCTGCAGTGG 
TGCGGGCTGA CGGAGGGTGA CAGCGGGGCG CTGGGGATGC TGCTGGCCAC GCTCCCCAGC 
CTGGTCCACT TGGAGCTGGG CGACGGAGCG CTGGGTGATG ATGGTGTGAG GATGCTCTGT 
GCCGGGCTGC GCCAACCCGG CTGCCAGCTG CGTGTCCTGC GGCTGCGGTA CACCCACCTC 
ACCAGTGCCT GCTGCCAGGA CCTCGCCGTG GCACTGGGCA CCAGCACGTG CCTGGAGGAG 
CTGGACCTGT CCTTCAGCGC CGGGCTGCGG GATGACGGCG TGAAGCTGCT GTGTGAGGGG 
CTCCAGCACA GCGGCTGCCA ACTGAGGGTG CTGCGGCTGG GGAGCTGCAG CCTGACCGGC 
GCCTGCTGCC AGGCCCTGGT GGCATGGCTC GGGCACAGCC ACGGCCTGAA GTGCCTGGAC 
CTGAGTGACA CCGAGCTGGG AGCTGGAGCC ACGCTGCTGC TGCAGCATCT CCGCCATCAC 
AGCTGCACGC TGCAGACGCT GGGGCTGAGC ACTTCTACAC TGAGCAAGGA TGCGCTGCAG 
GAGCTGGCTG CCCTCCGGGC GCTGAAGCCC AGCTTGAAGA TCACAGACCT ACTGGAACAC 
GAGGCCCCAG AGACGGGTGC CATGGCACGA CTGACCTTCC AGCGCAGCGT CTGGGCGGGC 
AGGGGGGCAG CTGTTCGGGG GCGAAAGGGG CTCCCCTCCT CTCGGGCAGC CCCTCCCCAC 
AGCAGGAGCC TGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tissue-specific expression pattern and histological distribution of NLRP3 in Chinese yellow chicken.
Veterinary research communications39(3)171-7(2015 Sep)
Ye J,Yu M,Zhang K,Liu J,Wang Q,Tao P,Jia K,Liao M,Ning Z

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

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NLRP3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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