LOC416696 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC416696
Entrez Gene ID	416696
Full Name	collagen, type VII, alpha 1-like
Synonyms	COL7A1
General protein information	Preferred Names collagen, type VII, alpha 1-like Names collagen alpha-1(VII) chain-like collagen, type VII, alpha 1-like collagen alpha-1(VII) chain-like; collagen, type VII, alpha 1-like collagen, type VII, alpha 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015286002.1	XP_015141488.1	collagen alpha-1(VII) chain-like isoform X3
XM_015286002.2	XP_015141488.2	collagen alpha-1(VII) chain-like isoform X1
XM_015286087.1	XP_015141573.1	collagen alpha-1(VII) chain-like isoform X5
XM_004937436.2	XP_004937493.2	collagen alpha-1(VII) chain-like isoform X1
XM_004937437.2	XP_004937494.2	collagen alpha-1(VII) chain-like isoform X2
XM_025153151.1	XP_025008919.1	collagen alpha-1(VII) chain-like isoform X2
XM_025153153.1	XP_025008921.1	collagen alpha-1(VII) chain-like isoform X3
XM_015286043.1	XP_015141529.1	collagen alpha-1(VII) chain-like isoform X4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC416696	XP_004937493.1
Xenopus tropicalis (tropical clawed frog)	LOC101732997	XP_004913247.1

Gene Ontology

The following LOC416696 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC416696 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa01289	XM_015286087.1 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa28781	XM_004937436.2 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa28782	XM_004937437.2 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa28783	XM_015286002.1 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa28784	XM_015286043.1 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa142490	XM_015286002.2 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa142491	XM_025153151.1 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa142492	XM_025153153.1 Latest version!	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC416696 ( XM_015286087.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141573.1 Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa01289
Clone ID Related Accession (Same CDS sequence)	XM_015286087.1
Accession Version	XM_015286087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455697.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGGAGAACCA GGGAAGAAAG GCACCTCTGG GATGAAGGGA GAAGCTGGTG CCCCAGGAGA 
ACCAGGAGAA AGAGGAATGA GGGGTCCACT TGGACTCCCG GGGCGACCTG GAGAGCAGGG 
GATAAAAGGC GACCCAGGAG AACCTGGAAA GGATGGTATG CCTGGGCTGC CTGGGACTCC 
TGGAAGCATT ATGTCTTCTC TGGAGAATAC CATCACTCTG AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141573.1
CDS	614..5341
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGRTREERHLWDEGRSWCPRRTRRKRNEGSTWTPGATWRAGDKRRPRRTWKGWYAWAAWDSWKHYVFSGEYHHSER

Target ORF information:

RefSeq Version	XM_015286087.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286087.1

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGGAGAACCA GGGAAGAAAG GCACCTCTGG GATGAAGGGA GAAGCTGGTG CCCCAGGAGA 
ACCAGGAGAA AGAGGAATGA GGGGTCCACT TGGACTCCCG GGGCGACCTG GAGAGCAGGG 
GATAAAAGGC GACCCAGGAG AACCTGGAAA GGATGGTATG CCTGGGCTGC CTGGGACTCC 
TGGAAGCATT ATGTCTTCTC TGGAGAATAC CATCACTCTG AAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_004937436.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937493.2 Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa28781
Clone ID Related Accession (Same CDS sequence)	XM_004937436.2
Accession Version	XM_004937436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455697.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGGCCC 
TCCAGATCCA CCCTACCATT GCCAAAAGGG AGAAATGGGA GCTCCAGGAG CCCGTGGAAA 
CAAAGGAGAA AAGGTAGCAA TAGCGCTTTT GTGAGCCTCT GGGACTTGGA GAAGGCTCCC 
TGTGCTAATA GGGTCTGGAA GATAAGAAGC TGGGACATAC CGGTGGAGTG GACATTCAGG 
GTCTGTGGGA GATTGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937493.2
CDS	613..6393
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGITGEKGDKGESGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPSRSTLPLPKGRNGSSRSPWKQRRKGSNSAFVSLWDLEKAPCANRVWKIRSWDIPVEWTFRVCGRLC

Target ORF information:

RefSeq Version	XM_004937436.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937436.2

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGGCCC 
TCCAGATCCA CCCTACCATT GCCAAAAGGG AGAAATGGGA GCTCCAGGAG CCCGTGGAAA 
CAAAGGAGAA AAGGTAGCAA TAGCGCTTTT GTGAGCCTCT GGGACTTGGA GAAGGCTCCC 
TGTGCTAATA GGGTCTGGAA GATAAGAAGC TGGGACATAC CGGTGGAGTG GACATTCAGG 
GTCTGTGGGA GATTGTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_004937437.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937494.2 Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X2, mRNA.

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CloneID	OGa28782
Clone ID Related Accession (Same CDS sequence)	XM_004937437.2
Accession Version	XM_004937437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455697.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGTATGCC TGGGCTGCCT 
GGGACTCCTG GAAGCATTAT GTCTTCTCTG GAGAATACCA TCACTCTGAA AGGTGAAAAG 
GGAGATCGAG GGAAGGATGG TTTGAAAGGT GAAAGAGGTC CTCCAGGCCC AAAGGGAGAG 
CCTGGTCCAC CAGGAAAAGG AGTGGAAATG AAGGATCTGG AGAGACTTTT TGAAGAAAAT 
GGTATCAAGC TGGCATTGCT GAAGGACCTC TCCAATGCAA TCCTGCAGGA AGGGCTGGAT 
TGGCTGATGC AGCACTTGGG CAGCTCCAGG ACCTCCAGGA GAAAGCAGGC ACTTTCACAT 
ACACCCAAAC ATGGCGGTTC ATTGACAGCT GATACTCATG ATGTCCTGAC CAGCACTGGG 
GTGACAGAAG AAGCACAGAT TCTGGCAGTG GACACTCCAT TTAGGGTGCT GGTGTCTGAA 
GGACTCCCTA AGGGTCCATC TGATGATGGT TCTGATTCTG AGCACCAACC CCTGGGGAAC 
GCTCCTGAGT CACTGAAGAA AATATCTGAG GAAAGAAGGA AGGAGGGCGA CTCAGAAGGG 
ACAAATATCT CTCTCAGCAG TAGCTTCTCT ATGTCCTCAC CACATCCAGA AGGTGATCTG 
CAAGGAAACC TGTCCATAGA GCTTCTCGAA TCCTTGGAGG AAAGAGTGGA AGGACAACCA 
CAAGAGGACA ACAGCCTTGA AAACATGAAT TCAGAAGATG GCTCTGCACT CCCAAGGCAT 
CCTAGAGCCA GGAGATCAAC CCAGCATGAT ACACACATGG TTCACCCTCC CGGTGCCCCA 
GGAGCTCCAA AATTCCCATC TGGATCCATG ACATACCCAG GTCCTCAGCA CCCGAGGAGA 
ATTAAGAAGG AGGGAATGGG GCCCTCCAGA TCCACCCTAC CATTGCCAAA AGGGAGAAAT 
GGGAGCTCCA GGAGCCCGTG GAAACAAAGG AGAAAAGGTA GCAATAGCGC TTTTGTGAGC 
CTCTGGGACT TGGAGAAGGC TCCCTGTGCT AATAGGGTCT GGAAGATAAG AAGCTGGGAC 
ATACCGGTGG AGTGGACATT CAGGGTCTGT GGGAGATTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937494.2
CDS	613..6357
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPSRSTLPLPKGRNGSSRSPWKQRRKGSNSAFVSLWDLEKAPCANRVWKIRSWDIPVEWTFRVCGRLC

Target ORF information:

RefSeq Version	XM_004937437.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937437.2

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGTATGCC TGGGCTGCCT 
GGGACTCCTG GAAGCATTAT GTCTTCTCTG GAGAATACCA TCACTCTGAA AGGTGAAAAG 
GGAGATCGAG GGAAGGATGG TTTGAAAGGT GAAAGAGGTC CTCCAGGCCC AAAGGGAGAG 
CCTGGTCCAC CAGGAAAAGG AGTGGAAATG AAGGATCTGG AGAGACTTTT TGAAGAAAAT 
GGTATCAAGC TGGCATTGCT GAAGGACCTC TCCAATGCAA TCCTGCAGGA AGGGCTGGAT 
TGGCTGATGC AGCACTTGGG CAGCTCCAGG ACCTCCAGGA GAAAGCAGGC ACTTTCACAT 
ACACCCAAAC ATGGCGGTTC ATTGACAGCT GATACTCATG ATGTCCTGAC CAGCACTGGG 
GTGACAGAAG AAGCACAGAT TCTGGCAGTG GACACTCCAT TTAGGGTGCT GGTGTCTGAA 
GGACTCCCTA AGGGTCCATC TGATGATGGT TCTGATTCTG AGCACCAACC CCTGGGGAAC 
GCTCCTGAGT CACTGAAGAA AATATCTGAG GAAAGAAGGA AGGAGGGCGA CTCAGAAGGG 
ACAAATATCT CTCTCAGCAG TAGCTTCTCT ATGTCCTCAC CACATCCAGA AGGTGATCTG 
CAAGGAAACC TGTCCATAGA GCTTCTCGAA TCCTTGGAGG AAAGAGTGGA AGGACAACCA 
CAAGAGGACA ACAGCCTTGA AAACATGAAT TCAGAAGATG GCTCTGCACT CCCAAGGCAT 
CCTAGAGCCA GGAGATCAAC CCAGCATGAT ACACACATGG TTCACCCTCC CGGTGCCCCA 
GGAGCTCCAA AATTCCCATC TGGATCCATG ACATACCCAG GTCCTCAGCA CCCGAGGAGA 
ATTAAGAAGG AGGGAATGGG GCCCTCCAGA TCCACCCTAC CATTGCCAAA AGGGAGAAAT 
GGGAGCTCCA GGAGCCCGTG GAAACAAAGG AGAAAAGGTA GCAATAGCGC TTTTGTGAGC 
CTCTGGGACT TGGAGAAGGC TCCCTGTGCT AATAGGGTCT GGAAGATAAG AAGCTGGGAC 
ATACCGGTGG AGTGGACATT CAGGGTCTGT GGGAGATTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_015286002.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141488.1 Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X3, mRNA.

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CloneID	OGa28783
Clone ID Related Accession (Same CDS sequence)	XM_015286002.1
Accession Version	XM_015286002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455697.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGGCCC 
TCCAGATCCA CCCTACCATT GCCAAAAGGG AGAAATGGGA GCTCCAGGAG CCCGTGGAAA 
CAAAGGAGAA AAGGGTCAGC CTGGGGAGAT GGGGGAGCCT GGAGAAAAGG GCACCAAAGG 
AGACAGAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141488.1
CDS	613..6324
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGITGEKGDKGESGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPSRSTLPLPKGRNGSSRSPWKQRRKGSAWGDGGAWRKGHQRRQR

Target ORF information:

RefSeq Version	XM_015286002.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286002.1

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGGCCC 
TCCAGATCCA CCCTACCATT GCCAAAAGGG AGAAATGGGA GCTCCAGGAG CCCGTGGAAA 
CAAAGGAGAA AAGGGTCAGC CTGGGGAGAT GGGGGAGCCT GGAGAAAAGG GCACCAAAGG 
AGACAGAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_015286043.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141529.1 Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X4, mRNA.

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CloneID	OGa28784
Clone ID Related Accession (Same CDS sequence)	XM_015286043.1
Accession Version	XM_015286043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455697.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGGAGAACCA GGGAAGAAAG GCACCTCTGG GATGAAGGGA GAAGCTGGTG CCCCAGGAGA 
ACCAGGAGAA AGAGGAATGA GGGGTCCACT TGGACTCCCG GGGCGACCTG GAGAGCAGGG 
GATAAAAGGC GACCCAGGAG AACCTGGAAA GGATGGAATT ACTGGAGAGA AGGGAGACAA 
GGGCGAATCT GGTATGCCTG GGCTGCCTGG GACTCCTGGA AGCATTATGT CTTCTCTGGA 
GAATACCATC ACTCTGAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141529.1
CDS	614..5377
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGRTREERHLWDEGRSWCPRRTRRKRNEGSTWTPGATWRAGDKRRPRRTWKGWNYWREGRQGRIWYAWAAWDSWKHYVFSGEYHHSER

Target ORF information:

RefSeq Version	XM_015286043.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (LOC416696), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286043.1

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGGAGAACCA GGGAAGAAAG GCACCTCTGG GATGAAGGGA GAAGCTGGTG CCCCAGGAGA 
ACCAGGAGAA AGAGGAATGA GGGGTCCACT TGGACTCCCG GGGCGACCTG GAGAGCAGGG 
GATAAAAGGC GACCCAGGAG AACCTGGAAA GGATGGAATT ACTGGAGAGA AGGGAGACAA 
GGGCGAATCT GGTATGCCTG GGCTGCCTGG GACTCCTGGA AGCATTATGT CTTCTCTGGA 
GAATACCATC ACTCTGAAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_015286002.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141488.2 Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X1, mRNA.

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CloneID	OGa142490
Clone ID Related Accession (Same CDS sequence)	XM_015286002.2
Accession Version	XM_015286002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015286002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGCCCT 
CCAGATCCAC CCTACCATTG CCAAAAGGGA GAAATGGGAG CTCCAGGAGC CCGTGGAAAC 
AAAGGAGAAA AGGGTCAGCC TGGGGAGATG GGGGAGCCTG GAGAAAAGGG CACCAAAGGA 
GACAGAGGTG AAAGTGGTCA GAAGGGGGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGGC CCCCAGGACT TAAGGGTGAG CCAGGGGCCC CAGGACCTCC AGGAGCCCAG 
GGCATTCAGG GCATTCGTGG AAACGCTGGC ATTCCTGGCT CACAGGGAGA CAGAGGGGCT 
CCAGGTCTAC CCGGGACACC AGGACAGAAG GGTGAGAGTG GGAAGAGAGG CCGGAATGGA 
CTTGCTGGGC CTGCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTCC TGGGACACCT 
GGGCCCAAAG GCAACAAGGG AGAAACTGGG ATTGGAGTTG TTGGTCCAAG AGGTCCCCGA 
GGATTGCCCG GTTCTCAAGG TGACGAGGGC ATCATGGGAG TCCAGGGCCC CACTGGTATG 
ATGGGTCTAA AAGGTCTCCC GGGTGTGAAA GGTGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAGGGAG AAAGAGGTGA GACCATGACT GGTTTCGGAC CCCAAGGCTA CAAAGGCAGT 
AAAGGAGATC CTGGTGAACA AGGCCTGCCA GGTTTTGATG GAGACAAAGG CGAGAAGGGA 
GAGGATGGGC CTGCAGGAGA AAAGGGAATC AGAGGTGAAG CTGGCTCCAA GGGAGCCATG 
GGGCTTTTTG GAACAAGAGG ACCAGTAGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCCTG 
CTGGGCTCTG CCGGAACAGC AGGCCTCGAT GGGAGGAATG GCATCAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAAGGACA GAAGGGGAAG CCGGGAGAAA AAGGTGATCC TGGGATCACT 
GGGGACATTG GGCGGAAAGG AACAAAGGGC TTACGTGGGC TGCCAGGACG CATTGGTGCT 
CCAGGAGCCA AGGGCATCAA GGGAGAAATT GGCAGTCCTG GAAAGCCAGG AATCCCAGGA 
TCAGATGGAC CATACGGACC AAAGGGTGAA CAAGGAGCAC CAGGTGATGA TGGTGCCTTT 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA TCAAAGGGAG TTCCTGGTTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGAAAAGCC GGAGCTGCCG GACCAGATGG ACCTCAAGGG 
CCCCAGGGCA AACCAGGACC ACAGGGTCCA CGAGGGAGAA GAGGAAGACC TCAGCTCTGC 
CTCAGAGGTC CTCCGGGCAT GGATGGGCAA AAAGGAGTAA TGGGTGAAAA TGGTCCAGTT 
GGTCAGAAAG GAGAAAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCCA ATGCGATTGT GGAGATGAGC TGGGAAGGAT TCTTGTGGAA 
AGGGGCCTGG AAGCAATTGA ATTCAAGAGG CATCTCCAAG GGTATTTTCA GCCTCAAAAA 
CACTCTCAAA GGGATGGTGA AAACCAGGAA GAAATGTCCA ACAAAACCCA GAGCATGACC 
ACGTGGATGA CATCATCAGA GTCCTTCCTG GAGGCACCTT CTCTTACGAC AGCAGCTTCC 
AGCGTGAGGC CGATAACAGG TCTTCCTCTG TACAAGCGCC GAGAAGCTCA CAATCAATTA 
CCAGCCCCCT GCCTACAGCC AATGGATGAG GGCTCCTGTC GACACTACAC CCTGCGCTGG 
TACTTCCACC CAGCAGCAAA CGCCTGTCGA CCCTTCATCT TTGGGGGATG CCAAGGGAAC 
AGCAACCGCT TTGAAACCAA GAGGAAGTGT GAACGGTGGT GCAAAACCTC AGCAGTCAGA 
GGGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141488.2
CDS	645..8153
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGITGEKGDKGESGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPPDPPYHCQKGEMGAPGARGNKGEKGQPGEMGEPGEKGTKGDRGESGQKGEPGIGFRGPVGQAGPPGLKGEPGAPGPPGAQGIQGIRGNAGIPGSQGDRGAPGLPGTPGQKGESGKRGRNGLAGPAGPSGSPGKEGIPGTPGPKGNKGETGIGVVGPRGPRGLPGSQGDEGIMGVQGPTGMMGLKGLPGVKGERGDRGLLGPKGERGETMTGFGPQGYKGSKGDPGEQGLPGFDGDKGEKGEDGPAGEKGIRGEAGSKGAMGLFGTRGPVGQKGEPGEPGLLGSAGTAGLDGRNGIKGAKGDRGLQGQKGKPGEKGDPGITGDIGRKGTKGLRGLPGRIGAPGAKGIKGEIGSPGKPGIPGSDGPYGPKGEQGAPGDDGAFGIPGEKGEKGSKGVPGLGGFKGQMGWPGKAGAAGPDGPQGPQGKPGPQGPRGRRGRPQLCLRGPPGMDGQKGVMGENGPVGQKGEKGDPGLSEEEVKEVVRSEMRGQCDCGDELGRILVERGLEAIEFKRHLQGYFQPQKHSQRDGENQEEMSNKTQSMTTWMTSSESFLEAPSLTTAASSVRPITGLPLYKRREAHNQLPAPCLQPMDEGSCRHYTLRWYFHPAANACRPFIFGGCQGNSNRFETKRKCERWCKTSAVRGG

Target ORF information:

RefSeq Version	XM_015286002.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286002.2

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGCCCT 
CCAGATCCAC CCTACCATTG CCAAAAGGGA GAAATGGGAG CTCCAGGAGC CCGTGGAAAC 
AAAGGAGAAA AGGGTCAGCC TGGGGAGATG GGGGAGCCTG GAGAAAAGGG CACCAAAGGA 
GACAGAGGTG AAAGTGGTCA GAAGGGGGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGGC CCCCAGGACT TAAGGGTGAG CCAGGGGCCC CAGGACCTCC AGGAGCCCAG 
GGCATTCAGG GCATTCGTGG AAACGCTGGC ATTCCTGGCT CACAGGGAGA CAGAGGGGCT 
CCAGGTCTAC CCGGGACACC AGGACAGAAG GGTGAGAGTG GGAAGAGAGG CCGGAATGGA 
CTTGCTGGGC CTGCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTCC TGGGACACCT 
GGGCCCAAAG GCAACAAGGG AGAAACTGGG ATTGGAGTTG TTGGTCCAAG AGGTCCCCGA 
GGATTGCCCG GTTCTCAAGG TGACGAGGGC ATCATGGGAG TCCAGGGCCC CACTGGTATG 
ATGGGTCTAA AAGGTCTCCC GGGTGTGAAA GGTGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAGGGAG AAAGAGGTGA GACCATGACT GGTTTCGGAC CCCAAGGCTA CAAAGGCAGT 
AAAGGAGATC CTGGTGAACA AGGCCTGCCA GGTTTTGATG GAGACAAAGG CGAGAAGGGA 
GAGGATGGGC CTGCAGGAGA AAAGGGAATC AGAGGTGAAG CTGGCTCCAA GGGAGCCATG 
GGGCTTTTTG GAACAAGAGG ACCAGTAGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCCTG 
CTGGGCTCTG CCGGAACAGC AGGCCTCGAT GGGAGGAATG GCATCAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAAGGACA GAAGGGGAAG CCGGGAGAAA AAGGTGATCC TGGGATCACT 
GGGGACATTG GGCGGAAAGG AACAAAGGGC TTACGTGGGC TGCCAGGACG CATTGGTGCT 
CCAGGAGCCA AGGGCATCAA GGGAGAAATT GGCAGTCCTG GAAAGCCAGG AATCCCAGGA 
TCAGATGGAC CATACGGACC AAAGGGTGAA CAAGGAGCAC CAGGTGATGA TGGTGCCTTT 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA TCAAAGGGAG TTCCTGGTTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGAAAAGCC GGAGCTGCCG GACCAGATGG ACCTCAAGGG 
CCCCAGGGCA AACCAGGACC ACAGGGTCCA CGAGGGAGAA GAGGAAGACC TCAGCTCTGC 
CTCAGAGGTC CTCCGGGCAT GGATGGGCAA AAAGGAGTAA TGGGTGAAAA TGGTCCAGTT 
GGTCAGAAAG GAGAAAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCCA ATGCGATTGT GGAGATGAGC TGGGAAGGAT TCTTGTGGAA 
AGGGGCCTGG AAGCAATTGA ATTCAAGAGG CATCTCCAAG GGTATTTTCA GCCTCAAAAA 
CACTCTCAAA GGGATGGTGA AAACCAGGAA GAAATGTCCA ACAAAACCCA GAGCATGACC 
ACGTGGATGA CATCATCAGA GTCCTTCCTG GAGGCACCTT CTCTTACGAC AGCAGCTTCC 
AGCGTGAGGC CGATAACAGG TCTTCCTCTG TACAAGCGCC GAGAAGCTCA CAATCAATTA 
CCAGCCCCCT GCCTACAGCC AATGGATGAG GGCTCCTGTC GACACTACAC CCTGCGCTGG 
TACTTCCACC CAGCAGCAAA CGCCTGTCGA CCCTTCATCT TTGGGGGATG CCAAGGGAAC 
AGCAACCGCT TTGAAACCAA GAGGAAGTGT GAACGGTGGT GCAAAACCTC AGCAGTCAGA 
GGGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_025153151.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008919.1 Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X2, mRNA.

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CloneID	OGa142491
Clone ID Related Accession (Same CDS sequence)	XM_025153151.1
Accession Version	XM_025153151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGCCCT 
CCAGATCCAC CCTACCATTG CCAAAAGGGA GAAATGGGAG CTCCAGGAGC CCGTGGAAAC 
AAAGGAGAAA AGGGTCAGCC TGGGGAGATG GGGGAGCCTG GAGAAAAGGG CACCAAAGGA 
GACAGAGGTG AAAGTGGTCA GAAGGGGGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGGC CCCCAGGACT TAAGGGTGAG CCAGGGGCCC CAGGACCTCC AGGAGCCCAG 
GGCATTCAGG GCATTCGTGG AAACGCTGGC ATTCCTGGCT CACAGGGAGA CAGAGGGGCT 
CCAGGTCTAC CCGGGACACC AGGACAGAAG GGTGAGAGTG GGAAGAGAGG CCGGAATGGA 
CTTGCTGGGC CTGCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTCC TGGGACACCT 
GGGCCCAAAG GCAACAAGGG AGAAACTGGG ATTGGAGTTG TTGGTCCAAG AGGTCCCCGA 
GGATTGCCCG GTTCTCAAGG TGACGAGGGC ATCATGGGAG TCCAGGGCCC CACTGGTATG 
ATGGGTCTAA AAGGTCTCCC GGGTGTGAAA GGTGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAGGGAG AAAGAGGTGA GACCATGACT GGTTTCGGAC CCCAAGGCTA CAAAGGCAGT 
AAAGGAGATC CTGGTGAACA AGGCCTGCCA GGTTTTGATG GAGACAAAGG CGAGAAGGGA 
GAGGATGGGC CTGCAGGAGA AAAGGGAATC AGAGGTGAAG CTGGCTCCAA GGGAGCCATG 
GGGCTTTTTG GAACAAGAGG ACCAGTAGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCCTG 
CTGGGCTCTG CCGGAACAGC AGGCCTCGAT GGGAGGAATG GCATCAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAAGGACA GAAGGGGAAG CCGGGAGAAA AAGGTGATCC TGGGATCACT 
GGGGACATTG GGCGGAAAGG AACAAAGGGC TTACGTGGGC TGCCAGGACG CATTGGTGCT 
CCAGGAGCCA AGGGCATCAA GGGAGAAATT GGCAGTCCTG GAAAGCCAGG AATCCCAGGA 
TCAGATGGAC CATACGGACC AAAGGGTGAA CAAGGAGCAC CAGGTGATGA TGGTGCCTTT 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA TCAAAGGGAG TTCCTGGTTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGAAAAGCC GGAGCTGCCG GACCAGATGG ACCTCAAGGG 
CCCCAGGGCA AACCAGGACC ACAGGGTCCA CGAGGGAGAA GAGGAAGACC TCAGCTCTGC 
CTCAGAGGTC CTCCGGGCAT GGATGGGCAA AAAGGAGTAA TGGGTGAAAA TGGTCCAGTT 
GGTCAGAAAG GAGAAAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCCA ATGCGATTGT GGAGATGAGC TGGGAAGGAT TCTTGTGGAA 
AGGGGCCTGG AAGCAATTGA ATTCAAGAGG CATCTCCAAG GGTATTTTCA GCCTCAAAAA 
CACTCTCAAA GGGATGGTGA AAACCAGGAA GAAATGTCCA ACAAAACCCA GAGCATGACC 
ACGTGGATGA CATCATCAGA GTCCTTCCTG GAGGCACCTT CTCTTACGAC AGCTTCCAGC 
GTGAGGCCGA TAACAGGTCT TCCTCTGTAC AAGCGCCGAG AAGCTCACAA TCAATTACCA 
GCCCCCTGCC TACAGCCAAT GGATGAGGGC TCCTGTCGAC ACTACACCCT GCGCTGGTAC 
TTCCACCCAG CAGCAAACGC CTGTCGACCC TTCATCTTTG GGGGATGCCA AGGGAACAGC 
AACCGCTTTG AAACCAAGAG GAAGTGTGAA CGGTGGTGCA AAACCTCAGC AGTCAGAGGG 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008919.1
CDS	646..8151
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGITGEKGDKGESGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPPDPPYHCQKGEMGAPGARGNKGEKGQPGEMGEPGEKGTKGDRGESGQKGEPGIGFRGPVGQAGPPGLKGEPGAPGPPGAQGIQGIRGNAGIPGSQGDRGAPGLPGTPGQKGESGKRGRNGLAGPAGPSGSPGKEGIPGTPGPKGNKGETGIGVVGPRGPRGLPGSQGDEGIMGVQGPTGMMGLKGLPGVKGERGDRGLLGPKGERGETMTGFGPQGYKGSKGDPGEQGLPGFDGDKGEKGEDGPAGEKGIRGEAGSKGAMGLFGTRGPVGQKGEPGEPGLLGSAGTAGLDGRNGIKGAKGDRGLQGQKGKPGEKGDPGITGDIGRKGTKGLRGLPGRIGAPGAKGIKGEIGSPGKPGIPGSDGPYGPKGEQGAPGDDGAFGIPGEKGEKGSKGVPGLGGFKGQMGWPGKAGAAGPDGPQGPQGKPGPQGPRGRRGRPQLCLRGPPGMDGQKGVMGENGPVGQKGEKGDPGLSEEEVKEVVRSEMRGQCDCGDELGRILVERGLEAIEFKRHLQGYFQPQKHSQRDGENQEEMSNKTQSMTTWMTSSESFLEAPSLTTASSVRPITGLPLYKRREAHNQLPAPCLQPMDEGSCRHYTLRWYFHPAANACRPFIFGGCQGNSNRFETKRKCERWCKTSAVRGG

Target ORF information:

RefSeq Version	XM_025153151.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153151.1

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGAATTAC TGGAGAGAAG 
GGAGACAAGG GCGAATCTGG TATGCCTGGG CTGCCTGGGA CTCCTGGAAG CATTATGTCT 
TCTCTGGAGA ATACCATCAC TCTGAAAGGT GAAAAGGGAG ATCGAGGGAA GGATGGTTTG 
AAAGGTGAAA GAGGTCCTCC AGGCCCAAAG GGAGAGCCTG GTCCACCAGG AAAAGGAGTG 
GAAATGAAGG ATCTGGAGAG ACTTTTTGAA GAAAATGGTA TCAAGCTGGC ATTGCTGAAG 
GACCTCTCCA ATGCAATCCT GCAGGAAGGG CTGGATTGGC TGATGCAGCA CTTGGGCAGC 
TCCAGGACCT CCAGGAGAAA GCAGGCACTT TCACATACAC CCAAACATGG CGGTTCATTG 
ACAGCTGATA CTCATGATGT CCTGACCAGC ACTGGGGTGA CAGAAGAAGC ACAGATTCTG 
GCAGTGGACA CTCCATTTAG GGTGCTGGTG TCTGAAGGAC TCCCTAAGGG TCCATCTGAT 
GATGGTTCTG ATTCTGAGCA CCAACCCCTG GGGAACGCTC CTGAGTCACT GAAGAAAATA 
TCTGAGGAAA GAAGGAAGGA GGGCGACTCA GAAGGGACAA ATATCTCTCT CAGCAGTAGC 
TTCTCTATGT CCTCACCACA TCCAGAAGGT GATCTGCAAG GAAACCTGTC CATAGAGCTT 
CTCGAATCCT TGGAGGAAAG AGTGGAAGGA CAACCACAAG AGGACAACAG CCTTGAAAAC 
ATGAATTCAG AAGATGGCTC TGCACTCCCA AGGCATCCTA GAGCCAGGAG ATCAACCCAG 
CATGATACAC ACATGGTTCA CCCTCCCGGT GCCCCAGGAG CTCCAAAATT CCCATCTGGA 
TCCATGACAT ACCCAGGTCC TCAGCACCCG AGGAGAATTA AGAAGGAGGG AATGGGCCCT 
CCAGATCCAC CCTACCATTG CCAAAAGGGA GAAATGGGAG CTCCAGGAGC CCGTGGAAAC 
AAAGGAGAAA AGGGTCAGCC TGGGGAGATG GGGGAGCCTG GAGAAAAGGG CACCAAAGGA 
GACAGAGGTG AAAGTGGTCA GAAGGGGGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGGC CCCCAGGACT TAAGGGTGAG CCAGGGGCCC CAGGACCTCC AGGAGCCCAG 
GGCATTCAGG GCATTCGTGG AAACGCTGGC ATTCCTGGCT CACAGGGAGA CAGAGGGGCT 
CCAGGTCTAC CCGGGACACC AGGACAGAAG GGTGAGAGTG GGAAGAGAGG CCGGAATGGA 
CTTGCTGGGC CTGCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTCC TGGGACACCT 
GGGCCCAAAG GCAACAAGGG AGAAACTGGG ATTGGAGTTG TTGGTCCAAG AGGTCCCCGA 
GGATTGCCCG GTTCTCAAGG TGACGAGGGC ATCATGGGAG TCCAGGGCCC CACTGGTATG 
ATGGGTCTAA AAGGTCTCCC GGGTGTGAAA GGTGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAGGGAG AAAGAGGTGA GACCATGACT GGTTTCGGAC CCCAAGGCTA CAAAGGCAGT 
AAAGGAGATC CTGGTGAACA AGGCCTGCCA GGTTTTGATG GAGACAAAGG CGAGAAGGGA 
GAGGATGGGC CTGCAGGAGA AAAGGGAATC AGAGGTGAAG CTGGCTCCAA GGGAGCCATG 
GGGCTTTTTG GAACAAGAGG ACCAGTAGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCCTG 
CTGGGCTCTG CCGGAACAGC AGGCCTCGAT GGGAGGAATG GCATCAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAAGGACA GAAGGGGAAG CCGGGAGAAA AAGGTGATCC TGGGATCACT 
GGGGACATTG GGCGGAAAGG AACAAAGGGC TTACGTGGGC TGCCAGGACG CATTGGTGCT 
CCAGGAGCCA AGGGCATCAA GGGAGAAATT GGCAGTCCTG GAAAGCCAGG AATCCCAGGA 
TCAGATGGAC CATACGGACC AAAGGGTGAA CAAGGAGCAC CAGGTGATGA TGGTGCCTTT 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA TCAAAGGGAG TTCCTGGTTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGAAAAGCC GGAGCTGCCG GACCAGATGG ACCTCAAGGG 
CCCCAGGGCA AACCAGGACC ACAGGGTCCA CGAGGGAGAA GAGGAAGACC TCAGCTCTGC 
CTCAGAGGTC CTCCGGGCAT GGATGGGCAA AAAGGAGTAA TGGGTGAAAA TGGTCCAGTT 
GGTCAGAAAG GAGAAAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCCA ATGCGATTGT GGAGATGAGC TGGGAAGGAT TCTTGTGGAA 
AGGGGCCTGG AAGCAATTGA ATTCAAGAGG CATCTCCAAG GGTATTTTCA GCCTCAAAAA 
CACTCTCAAA GGGATGGTGA AAACCAGGAA GAAATGTCCA ACAAAACCCA GAGCATGACC 
ACGTGGATGA CATCATCAGA GTCCTTCCTG GAGGCACCTT CTCTTACGAC AGCTTCCAGC 
GTGAGGCCGA TAACAGGTCT TCCTCTGTAC AAGCGCCGAG AAGCTCACAA TCAATTACCA 
GCCCCCTGCC TACAGCCAAT GGATGAGGGC TCCTGTCGAC ACTACACCCT GCGCTGGTAC 
TTCCACCCAG CAGCAAACGC CTGTCGACCC TTCATCTTTG GGGGATGCCA AGGGAACAGC 
AACCGCTTTG AAACCAAGAG GAAGTGTGAA CGGTGGTGCA AAACCTCAGC AGTCAGAGGG 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC416696 ( XM_025153153.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008921.1 Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X3, mRNA.

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CloneID	OGa142492
Clone ID Related Accession (Same CDS sequence)	XM_025153153.1
Accession Version	XM_025153153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(VII) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGTATGCC TGGGCTGCCT 
GGGACTCCTG GAAGCATTAT GTCTTCTCTG GAGAATACCA TCACTCTGAA AGGTGAAAAG 
GGAGATCGAG GGAAGGATGG TTTGAAAGGT GAAAGAGGTC CTCCAGGCCC AAAGGGAGAG 
CCTGGTCCAC CAGGAAAAGG AGTGGAAATG AAGGATCTGG AGAGACTTTT TGAAGAAAAT 
GGTATCAAGC TGGCATTGCT GAAGGACCTC TCCAATGCAA TCCTGCAGGA AGGGCTGGAT 
TGGCTGATGC AGCACTTGGG CAGCTCCAGG ACCTCCAGGA GAAAGCAGGC ACTTTCACAT 
ACACCCAAAC ATGGCGGTTC ATTGACAGCT GATACTCATG ATGTCCTGAC CAGCACTGGG 
GTGACAGAAG AAGCACAGAT TCTGGCAGTG GACACTCCAT TTAGGGTGCT GGTGTCTGAA 
GGACTCCCTA AGGGTCCATC TGATGATGGT TCTGATTCTG AGCACCAACC CCTGGGGAAC 
GCTCCTGAGT CACTGAAGAA AATATCTGAG GAAAGAAGGA AGGAGGGCGA CTCAGAAGGG 
ACAAATATCT CTCTCAGCAG TAGCTTCTCT ATGTCCTCAC CACATCCAGA AGGTGATCTG 
CAAGGAAACC TGTCCATAGA GCTTCTCGAA TCCTTGGAGG AAAGAGTGGA AGGACAACCA 
CAAGAGGACA ACAGCCTTGA AAACATGAAT TCAGAAGATG GCTCTGCACT CCCAAGGCAT 
CCTAGAGCCA GGAGATCAAC CCAGCATGAT ACACACATGG TTCACCCTCC CGGTGCCCCA 
GGAGCTCCAA AATTCCCATC TGGATCCATG ACATACCCAG GTCCTCAGCA CCCGAGGAGA 
ATTAAGAAGG AGGGAATGGG CCCTCCAGAT CCACCCTACC ATTGCCAAAA GGGAGAAATG 
GGAGCTCCAG GAGCCCGTGG AAACAAAGGA GAAAAGGGTC AGCCTGGGGA GATGGGGGAG 
CCTGGAGAAA AGGGCACCAA AGGAGACAGA GGTGAAAGTG GTCAGAAGGG GGAACCAGGA 
ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGGCCCCCAG GACTTAAGGG TGAGCCAGGG 
GCCCCAGGAC CTCCAGGAGC CCAGGGCATT CAGGGCATTC GTGGAAACGC TGGCATTCCT 
GGCTCACAGG GAGACAGAGG GGCTCCAGGT CTACCCGGGA CACCAGGACA GAAGGGTGAG 
AGTGGGAAGA GAGGCCGGAA TGGACTTGCT GGGCCTGCAG GACCATCAGG ATCCCCAGGA 
AAAGAGGGGA TTCCTGGGAC ACCTGGGCCC AAAGGCAACA AGGGAGAAAC TGGGATTGGA 
GTTGTTGGTC CAAGAGGTCC CCGAGGATTG CCCGGTTCTC AAGGTGACGA GGGCATCATG 
GGAGTCCAGG GCCCCACTGG TATGATGGGT CTAAAAGGTC TCCCGGGTGT GAAAGGTGAA 
AGAGGTGATC GGGGACTTCT GGGACCCAAG GGAGAAAGAG GTGAGACCAT GACTGGTTTC 
GGACCCCAAG GCTACAAAGG CAGTAAAGGA GATCCTGGTG AACAAGGCCT GCCAGGTTTT 
GATGGAGACA AAGGCGAGAA GGGAGAGGAT GGGCCTGCAG GAGAAAAGGG AATCAGAGGT 
GAAGCTGGCT CCAAGGGAGC CATGGGGCTT TTTGGAACAA GAGGACCAGT AGGACAGAAG 
GGGGAGCCAG GAGAACCAGG CCTGCTGGGC TCTGCCGGAA CAGCAGGCCT CGATGGGAGG 
AATGGCATCA AGGGAGCCAA AGGTGACAGA GGTCTTCAAG GACAGAAGGG GAAGCCGGGA 
GAAAAAGGTG ATCCTGGGAT CACTGGGGAC ATTGGGCGGA AAGGAACAAA GGGCTTACGT 
GGGCTGCCAG GACGCATTGG TGCTCCAGGA GCCAAGGGCA TCAAGGGAGA AATTGGCAGT 
CCTGGAAAGC CAGGAATCCC AGGATCAGAT GGACCATACG GACCAAAGGG TGAACAAGGA 
GCACCAGGTG ATGATGGTGC CTTTGGGATT CCTGGGGAGA AAGGAGAAAA GGGATCAAAG 
GGAGTTCCTG GTTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGAAA AGCCGGAGCT 
GCCGGACCAG ATGGACCTCA AGGGCCCCAG GGCAAACCAG GACCACAGGG TCCACGAGGG 
AGAAGAGGAA GACCTCAGCT CTGCCTCAGA GGTCCTCCGG GCATGGATGG GCAAAAAGGA 
GTAATGGGTG AAAATGGTCC AGTTGGTCAG AAAGGAGAAA AGGGAGATCC TGGTCTTTCT 
GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCCAATGCGA TTGTGGAGAT 
GAGCTGGGAA GGATTCTTGT GGAAAGGGGC CTGGAAGCAA TTGAATTCAA GAGGCATCTC 
CAAGGGTATT TTCAGCCTCA AAAACACTCT CAAAGGGATG GTGAAAACCA GGAAGAAATG 
TCCAACAAAA CCCAGAGCAT GACCACGTGG ATGACATCAT CAGAGTCCTT CCTGGAGGCA 
CCTTCTCTTA CGACAGCAGC TTCCAGCGTG AGGCCGATAA CAGGTCTTCC TCTGTACAAG 
CGCCGAGAAG CTCACAATCA ATTACCAGCC CCCTGCCTAC AGCCAATGGA TGAGGGCTCC 
TGTCGACACT ACACCCTGCG CTGGTACTTC CACCCAGCAG CAAACGCCTG TCGACCCTTC 
ATCTTTGGGG GATGCCAAGG GAACAGCAAC CGCTTTGAAA CCAAGAGGAA GTGTGAACGG 
TGGTGCAAAA CCTCAGCAGT CAGAGGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008921.1
CDS	645..8117
Translation	MYAQILALAVAVAHYRAMAVHEVCRTAEVADVLFLVGHSQGAGRESSQLLKDFISSMARSFENVVMGKGGIRMAVALYGEKPRMSIELTDYVTIEEILVAIQEISIKGSSLKVGSALAFAAHAMSQPATLRDNAAKVVVLITSGKSSDLVEDKAQVLQDAGVTVFAVGIKDADKHELNKIASEPTAEHVIYVDDFHLLHSAAPKLSRRLCFTASEPPRPIKQPVQDEKIIGPRDLHVSEHSHSSLRFTWMPATGRVIGYRVHVHPLLPSGQPVLEDQQQILVDGDKCTVLVTDLKPNTKYVFTVRAIYTDALGESAAVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKVYIPGANRPGMTYERVLHGDVSSHVLDNLQEDREYSISIYAVYPQGPSQPVTAMGRTLKLLPVKSILLQNETTDTLQARWSPVRGATGYRLTWTSADGSIQDVNLSSTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSRKATTVASSTVQMLNVSEISTNGALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNKSITTYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYISNFKVTSYTSSSLSLSWNAMLAATKFKVTWRPVGARNEKQVAKTQYLGSRLLAHQIDHLLPDTLYKVSIRAVFSKTEGPEVTLTHRTASLADSSPIHTIQDLRITNTAVNSLKLSWRRLPGATHYKISWVPFSGGLETSQLVGAESNSFTISNLKESTTYTIGVSAVIGGQEGNPTLLTAKTLDLPKVTEFTLQEAAETSVLLTWAAVPGVSTYILTWRLSSASDLSLEVLSAALRSYRVRGLSSEETYLFSIRPVFGDREGPETTLIGQTAGPHGDSPPPASPMTSAKLATMRSPSPSSVRTNPLPLATATLPPTPNTRLSSTTASAPLTALPGPICGKFKADIGFLVDESSSIGWSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFHFNQHQDRNGALKAVKELHYAGGNTKTGRGIAFMLKELFQPSRGMRPEFPHVLMLVTDGRSQDDVLPPARAAHALGIRIIAVGVSGADPAELNDILLQQNLQNVFYVSTFDEFPQILRELIEVICSDPQPSGALLPSGEVARPEADKQLPFDDTDNKVHLPSPSPAEPDIQKGVETCGPWCLKNIRGSPARGGYDPYGFTMKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDIGSPGYPGPVGPKGDRGEPGYVLGGVEVIPGRNGHPGTPGQKGQPGVPGLAGPQGLPGLPGPQGPPGLSIKGEPGDSGLRGPRGRNGIKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPKGEKGEKGTAGIGIPGTAGPKGEEGQQGVMGPPGVPGPKGLPGPPGQKGVQVLGPPGKKGVRGDIGPTGAPGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDTGDRGNVGLSGRPGYKGEIGPKGEKGEPGLPGKPGETGLRGKDGEPGKKGTSGMKGEAGAPGEPGERGMRGPLGLPGRPGEQGIKGDPGEPGKDGMPGLPGTPGSIMSSLENTITLKGEKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEENGIKLALLKDLSNAILQEGLDWLMQHLGSSRTSRRKQALSHTPKHGGSLTADTHDVLTSTGVTEEAQILAVDTPFRVLVSEGLPKGPSDDGSDSEHQPLGNAPESLKKISEERRKEGDSEGTNISLSSSFSMSSPHPEGDLQGNLSIELLESLEERVEGQPQEDNSLENMNSEDGSALPRHPRARRSTQHDTHMVHPPGAPGAPKFPSGSMTYPGPQHPRRIKKEGMGPPDPPYHCQKGEMGAPGARGNKGEKGQPGEMGEPGEKGTKGDRGESGQKGEPGIGFRGPVGQAGPPGLKGEPGAPGPPGAQGIQGIRGNAGIPGSQGDRGAPGLPGTPGQKGESGKRGRNGLAGPAGPSGSPGKEGIPGTPGPKGNKGETGIGVVGPRGPRGLPGSQGDEGIMGVQGPTGMMGLKGLPGVKGERGDRGLLGPKGERGETMTGFGPQGYKGSKGDPGEQGLPGFDGDKGEKGEDGPAGEKGIRGEAGSKGAMGLFGTRGPVGQKGEPGEPGLLGSAGTAGLDGRNGIKGAKGDRGLQGQKGKPGEKGDPGITGDIGRKGTKGLRGLPGRIGAPGAKGIKGEIGSPGKPGIPGSDGPYGPKGEQGAPGDDGAFGIPGEKGEKGSKGVPGLGGFKGQMGWPGKAGAAGPDGPQGPQGKPGPQGPRGRRGRPQLCLRGPPGMDGQKGVMGENGPVGQKGEKGDPGLSEEEVKEVVRSEMRGQCDCGDELGRILVERGLEAIEFKRHLQGYFQPQKHSQRDGENQEEMSNKTQSMTTWMTSSESFLEAPSLTTAASSVRPITGLPLYKRREAHNQLPAPCLQPMDEGSCRHYTLRWYFHPAANACRPFIFGGCQGNSNRFETKRKCERWCKTSAVRGG

Target ORF information:

RefSeq Version	XM_025153153.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen, type VII, alpha 1-like (COL7A1L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153153.1

ORF Insert Sequence:

ATGTACGCCC AGATCCTTGC CCTGGCAGTG GCTGTAGCAC ATTACAGAGC CATGGCTGTT 
CATGAAGTGT GCAGAACAGC AGAGGTGGCT GATGTCTTGT TCCTCGTGGG GCATTCCCAG 
GGTGCAGGGA GAGAGAGCTC CCAACTGCTC AAGGACTTCA TCAGCAGCAT GGCCCGCTCC 
TTTGAGAATG TGGTGATGGG CAAAGGAGGC ATCCGGATGG CAGTGGCACT CTATGGAGAG 
AAACCAAGGA TGAGCATTGA ACTGACGGAT TATGTGACCA TCGAAGAAAT ACTGGTGGCA 
ATTCAGGAGA TTTCAATCAA AGGCAGCAGC TTGAAAGTGG GGTCTGCCCT GGCATTTGCA 
GCTCATGCCA TGTCTCAGCC AGCCACACTG AGAGACAATG CAGCAAAGGT AGTTGTTTTG 
ATCACGAGTG GGAAATCCAG TGACTTGGTT GAAGACAAAG CCCAAGTCCT GCAGGATGCA 
GGAGTGACCG TGTTTGCTGT AGGAATTAAG GATGCTGACA AGCATGAACT GAACAAAATA 
GCTTCGGAAC CTACAGCAGA GCATGTGATT TACGTTGATG ACTTTCACCT GCTCCACAGT 
GCAGCCCCCA AACTCTCACG AAGGCTCTGT TTCACTGCCT CGGAGCCCCC ACGGCCCATC 
AAACAACCTG TGCAAGATGA GAAGATCATC GGCCCTCGGG ATCTGCATGT CAGTGAGCAC 
AGCCACAGCT CACTGAGGTT CACGTGGATG CCAGCCACAG GGAGGGTGAT TGGGTACCGT 
GTCCACGTGC ATCCCTTGTT GCCTTCAGGA CAGCCAGTTC TGGAGGATCA GCAGCAGATT 
TTGGTGGATG GTGACAAATG CACTGTGCTG GTGACTGATC TGAAACCCAA CACCAAGTAC 
GTCTTCACAG TGAGAGCCAT CTATACAGAT GCCCTCGGGG AGTCAGCAGC TGTCAAGGGG 
AAAACCACAC CAGTGCCTCC AGTCACTAAT TTCCGTGTGA TTGAAGAAGG ACTTTTTAGC 
TTGAAGGTTG CTTGGACTCC TCCCTTGGGC AAGCTGGAAG GCTACAAAGT CTACATCCCC 
GGAGCCAATA GACCTGGAAT GACCTACGAG CGGGTTCTGC ATGGTGATGT CTCCTCCCAT 
GTGCTAGATA ACTTGCAGGA GGATAGAGAG TACAGCATCA GCATCTATGC AGTGTACCCC 
CAGGGTCCAA GCCAGCCTGT GACTGCCATG GGGAGAACAT TGAAGCTCCT GCCTGTGAAG 
AGCATCCTGC TGCAGAATGA GACCACTGAT ACACTCCAGG CACGGTGGAG CCCTGTCCGG 
GGAGCAACGG GCTACAGGCT CACCTGGACA TCAGCAGATG GCAGCATCCA GGATGTGAAC 
CTCAGCAGCA CCTACAGCTA CTACATGATC CAGGGGCTGC AGCCTGGCAC AGAATACACT 
GTCACCATCA ACCCCATCTT TGGAGATGTT GAGGGGCCAG TGGTGAGCAG AAAAGCAACC 
ACAGTCGCTT CAAGCACTGT GCAGATGCTG AATGTGAGTG AGATATCCAC CAATGGTGCT 
CTGGTCTCCT GGGACTCGGT TGCTGGGGCC ACTGGGTACC GAGTGGCTTG GGGTCCTACA 
CCAGAATTCT TTGGGAAAGA CCGACCCCGG CAGCTGGCCC TCAACAAGTC AATCACAACC 
TACCAGCTCC GCAACCTGGC CCATGACACC GAGTACGTGA TTTCCCTCTA TGTGCTCTTT 
GGTTCAGTGG AAGGACCAGG GATCACAACC TCAGCCAGGA CATCTCCTCT TGGCTACATC 
TCCAATTTCA AGGTGACATC CTACACCAGC TCAAGCCTGT CTCTGTCATG GAACGCCATG 
TTGGCTGCCA CCAAATTCAA AGTCACCTGG AGACCTGTGG GAGCCAGAAA TGAAAAACAA 
GTGGCCAAGA CTCAGTACCT GGGCAGTAGG TTGTTGGCTC ACCAGATTGA CCACCTGCTG 
CCTGACACCC TGTATAAAGT CAGCATTAGG GCTGTCTTCA GCAAGACTGA GGGTCCAGAG 
GTGACTCTGA CTCACCGCAC AGCATCTCTT GCAGACTCCA GCCCCATCCA CACCATCCAA 
GACCTGAGGA TTACCAACAC TGCTGTCAAT AGTTTGAAGC TGTCTTGGAG AAGGCTTCCA 
GGAGCAACTC ACTATAAGAT ATCCTGGGTG CCATTCAGTG GTGGCTTGGA AACCTCACAG 
TTAGTTGGAG CTGAGAGCAA CTCATTCACC ATCTCCAATC TGAAGGAGAG CACCACCTAC 
ACCATTGGCG TGTCTGCAGT GATTGGAGGC CAGGAGGGAA ACCCCACGCT CCTCACAGCC 
AAAACTTTGG ATTTGCCCAA AGTGACTGAG TTCACTCTTC AGGAAGCTGC AGAGACCAGT 
GTTTTGCTGA CTTGGGCTGC TGTTCCTGGA GTATCCACAT ATATACTAAC ATGGCGCTTG 
TCCTCAGCTT CTGATCTTTC CCTGGAAGTG CTATCAGCAG CTTTGAGATC GTATCGAGTG 
AGAGGACTGA GCTCTGAAGA GACATATTTG TTTTCCATTA GACCGGTCTT TGGAGATAGA 
GAAGGACCAG AGACAACACT CATTGGGCAA ACAGCTGGAC CTCACGGCGA TTCACCTCCA 
CCAGCCTCTC CCATGACTTC TGCAAAACTG GCTACAATGA GATCCCCATC TCCAAGCAGT 
GTGAGGACCA ATCCTCTTCC ATTAGCCACC GCTACATTAC CACCAACCCC AAACACCCGG 
CTGTCATCTA CCACAGCTTC TGCACCACTG ACAGCTCTCC CTGGCCCAAT CTGTGGCAAG 
TTCAAAGCAG ACATTGGGTT CCTGGTGGAT GAGTCCTCAA GTATTGGCTG GAGTAATTTC 
AACAAAGTGA AGGATTTCCT CTTCCGGATC ATTTCCTATT TCCCTAAGAT TGGGCCTGAA 
GGAACACAGG TTGCTGTTGC ACAGTACAGT GAGGAGCCCA GAGCAGCCTT CCACTTCAAC 
CAGCACCAAG ACCGTAATGG TGCTCTGAAA GCTGTCAAGG AGCTGCACTA TGCTGGAGGC 
AACACGAAGA CGGGTCGTGG TATAGCCTTT ATGCTAAAGG AGTTATTTCA GCCCTCCAGA 
GGGATGAGAC CAGAGTTCCC TCATGTGCTG ATGCTTGTGA CTGATGGGCG ATCCCAGGAT 
GATGTGTTGC CACCTGCCAG AGCAGCCCAT GCTTTGGGAA TCCGCATCAT TGCTGTGGGT 
GTCTCGGGAG CTGACCCTGC TGAGCTCAAC GACATTTTAC TGCAGCAGAA CCTCCAGAAC 
GTTTTCTATG TGAGCACATT CGATGAGTTC CCCCAGATCC TCCGAGAGCT AATAGAAGTC 
ATTTGTTCTG ACCCTCAGCC TTCAGGGGCC CTGCTGCCAT CTGGGGAGGT TGCCAGACCT 
GAAGCTGACA AGCAATTGCC ATTTGATGAC ACAGACAACA AGGTACACCT CCCCAGTCCA 
TCACCAGCTG AACCAGACAT CCAGAAAGGA GTGGAGACTT GTGGTCCCTG GTGTCTAAAG 
AACATCAGGG GCTCACCTGC TCGTGGAGGC TATGACCCAT ATGGTTTTAC TATGAAGGGA 
GAGAAAGGAG AGAGGGGTCT ACCTGGGAAG GATGGCATAC CTGGACTGCC AGGCAGACCA 
GGGCGAACAG GTCCTCCTGG TCCCCCTGGA CTCATGGGTC TCCCTGGTAT CCAAGGTGAT 
ATTGGATCTC CAGGCTACCC GGGACCAGTG GGGCCAAAAG GAGACAGGGG AGAACCTGGT 
TATGTCCTGG GAGGAGTGGA GGTGATTCCT GGCCGAAATG GGCATCCCGG CACCCCTGGA 
CAGAAAGGAC AGCCGGGTGT TCCTGGGTTA GCAGGACCAC AGGGTTTACC TGGCTTACCA 
GGGCCACAGG GGCCACCAGG ACTCTCCATC AAGGGTGAAC CAGGGGATTC GGGTCTCAGA 
GGCCCTCGTG GAAGGAATGG CATCAAAGGG GACAGAGGAG GCATGGGAGA GCCGGGGAAA 
CCTGGCTTGC CAGGCCCTGT AGGGCTGGAT GGTGTTCCTG GACTGCCTGG ACCAAAGGGA 
GAAAAGGGAG AAAAGGGGAC AGCAGGAATT GGCATTCCTG GCACTGCTGG ACCAAAGGGA 
GAAGAAGGAC AGCAGGGGGT GATGGGTCCT CCAGGAGTAC CTGGACCAAA GGGACTTCCA 
GGACCTCCAG GGCAGAAGGG AGTCCAAGTT TTGGGACCAC CAGGCAAGAA AGGTGTCAGA 
GGAGATATTG GACCAACAGG AGCCCCAGGA CCACAAGGAG ACAAAGGAAT ACAAGGAGAC 
AAAGGAGAGA AGGGAAGTCC AGGTTTTGGC ATTCCTGGTC AACCAGGGCT GAAGGGAGAC 
ACAGGGGACA GGGGAAACGT AGGCTTGTCT GGCAGACCAG GTTACAAGGG GGAAATAGGT 
CCGAAGGGAG AGAAAGGTGA ACCAGGACTA CCTGGGAAAC CAGGCGAGAC TGGCCTAAGA 
GGTAAAGATG GAGAACCAGG GAAGAAAGGC ACCTCTGGGA TGAAGGGAGA AGCTGGTGCC 
CCAGGAGAAC CAGGAGAAAG AGGAATGAGG GGTCCACTTG GACTCCCGGG GCGACCTGGA 
GAGCAGGGGA TAAAAGGCGA CCCAGGAGAA CCTGGAAAGG ATGGTATGCC TGGGCTGCCT 
GGGACTCCTG GAAGCATTAT GTCTTCTCTG GAGAATACCA TCACTCTGAA AGGTGAAAAG 
GGAGATCGAG GGAAGGATGG TTTGAAAGGT GAAAGAGGTC CTCCAGGCCC AAAGGGAGAG 
CCTGGTCCAC CAGGAAAAGG AGTGGAAATG AAGGATCTGG AGAGACTTTT TGAAGAAAAT 
GGTATCAAGC TGGCATTGCT GAAGGACCTC TCCAATGCAA TCCTGCAGGA AGGGCTGGAT 
TGGCTGATGC AGCACTTGGG CAGCTCCAGG ACCTCCAGGA GAAAGCAGGC ACTTTCACAT 
ACACCCAAAC ATGGCGGTTC ATTGACAGCT GATACTCATG ATGTCCTGAC CAGCACTGGG 
GTGACAGAAG AAGCACAGAT TCTGGCAGTG GACACTCCAT TTAGGGTGCT GGTGTCTGAA 
GGACTCCCTA AGGGTCCATC TGATGATGGT TCTGATTCTG AGCACCAACC CCTGGGGAAC 
GCTCCTGAGT CACTGAAGAA AATATCTGAG GAAAGAAGGA AGGAGGGCGA CTCAGAAGGG 
ACAAATATCT CTCTCAGCAG TAGCTTCTCT ATGTCCTCAC CACATCCAGA AGGTGATCTG 
CAAGGAAACC TGTCCATAGA GCTTCTCGAA TCCTTGGAGG AAAGAGTGGA AGGACAACCA 
CAAGAGGACA ACAGCCTTGA AAACATGAAT TCAGAAGATG GCTCTGCACT CCCAAGGCAT 
CCTAGAGCCA GGAGATCAAC CCAGCATGAT ACACACATGG TTCACCCTCC CGGTGCCCCA 
GGAGCTCCAA AATTCCCATC TGGATCCATG ACATACCCAG GTCCTCAGCA CCCGAGGAGA 
ATTAAGAAGG AGGGAATGGG CCCTCCAGAT CCACCCTACC ATTGCCAAAA GGGAGAAATG 
GGAGCTCCAG GAGCCCGTGG AAACAAAGGA GAAAAGGGTC AGCCTGGGGA GATGGGGGAG 
CCTGGAGAAA AGGGCACCAA AGGAGACAGA GGTGAAAGTG GTCAGAAGGG GGAACCAGGA 
ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGGCCCCCAG GACTTAAGGG TGAGCCAGGG 
GCCCCAGGAC CTCCAGGAGC CCAGGGCATT CAGGGCATTC GTGGAAACGC TGGCATTCCT 
GGCTCACAGG GAGACAGAGG GGCTCCAGGT CTACCCGGGA CACCAGGACA GAAGGGTGAG 
AGTGGGAAGA GAGGCCGGAA TGGACTTGCT GGGCCTGCAG GACCATCAGG ATCCCCAGGA 
AAAGAGGGGA TTCCTGGGAC ACCTGGGCCC AAAGGCAACA AGGGAGAAAC TGGGATTGGA 
GTTGTTGGTC CAAGAGGTCC CCGAGGATTG CCCGGTTCTC AAGGTGACGA GGGCATCATG 
GGAGTCCAGG GCCCCACTGG TATGATGGGT CTAAAAGGTC TCCCGGGTGT GAAAGGTGAA 
AGAGGTGATC GGGGACTTCT GGGACCCAAG GGAGAAAGAG GTGAGACCAT GACTGGTTTC 
GGACCCCAAG GCTACAAAGG CAGTAAAGGA GATCCTGGTG AACAAGGCCT GCCAGGTTTT 
GATGGAGACA AAGGCGAGAA GGGAGAGGAT GGGCCTGCAG GAGAAAAGGG AATCAGAGGT 
GAAGCTGGCT CCAAGGGAGC CATGGGGCTT TTTGGAACAA GAGGACCAGT AGGACAGAAG 
GGGGAGCCAG GAGAACCAGG CCTGCTGGGC TCTGCCGGAA CAGCAGGCCT CGATGGGAGG 
AATGGCATCA AGGGAGCCAA AGGTGACAGA GGTCTTCAAG GACAGAAGGG GAAGCCGGGA 
GAAAAAGGTG ATCCTGGGAT CACTGGGGAC ATTGGGCGGA AAGGAACAAA GGGCTTACGT 
GGGCTGCCAG GACGCATTGG TGCTCCAGGA GCCAAGGGCA TCAAGGGAGA AATTGGCAGT 
CCTGGAAAGC CAGGAATCCC AGGATCAGAT GGACCATACG GACCAAAGGG TGAACAAGGA 
GCACCAGGTG ATGATGGTGC CTTTGGGATT CCTGGGGAGA AAGGAGAAAA GGGATCAAAG 
GGAGTTCCTG GTTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGAAA AGCCGGAGCT 
GCCGGACCAG ATGGACCTCA AGGGCCCCAG GGCAAACCAG GACCACAGGG TCCACGAGGG 
AGAAGAGGAA GACCTCAGCT CTGCCTCAGA GGTCCTCCGG GCATGGATGG GCAAAAAGGA 
GTAATGGGTG AAAATGGTCC AGTTGGTCAG AAAGGAGAAA AGGGAGATCC TGGTCTTTCT 
GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCCAATGCGA TTGTGGAGAT 
GAGCTGGGAA GGATTCTTGT GGAAAGGGGC CTGGAAGCAA TTGAATTCAA GAGGCATCTC 
CAAGGGTATT TTCAGCCTCA AAAACACTCT CAAAGGGATG GTGAAAACCA GGAAGAAATG 
TCCAACAAAA CCCAGAGCAT GACCACGTGG ATGACATCAT CAGAGTCCTT CCTGGAGGCA 
CCTTCTCTTA CGACAGCAGC TTCCAGCGTG AGGCCGATAA CAGGTCTTCC TCTGTACAAG 
CGCCGAGAAG CTCACAATCA ATTACCAGCC CCCTGCCTAC AGCCAATGGA TGAGGGCTCC 
TGTCGACACT ACACCCTGCG CTGGTACTTC CACCCAGCAG CAAACGCCTG TCGACCCTTC 
ATCTTTGGGG GATGCCAAGG GAACAGCAAC CGCTTTGAAA CCAAGAGGAA GTGTGAACGG 
TGGTGCAAAA CCTCAGCAGT CAGAGGGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC416696 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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