NCL cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCL
Entrez Gene ID	396201
Full Name	nucleolin
General protein information	Preferred Names nucleolin Names nucleolin protein C23
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205265.1	NP_990596.1	nucleolin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The PA-interacting host protein nucleolin acts as an antiviral factor during highly pathogenic H5N1 avian influenza virus infection.
Gao Z, Hu J, Wang X, Yang Q, Liang Y, Ma C, Liu D, Liu K, Hao X, Gu M, Liu X, Jiao XA, Liu X
Archives of virology163(10)2775-2786(2018 Oct)

Conditional knockout of nucleolin in DT40 cells reveals the functional redundancy of its RNA-binding domains.
Storck S, Thiry M, Bouvet P
Biology of the cell101(3)153-67(2009 Mar)

Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase.
Peter M, Nakagawa J, Dorée M, Labbé JC, Nigg EA
Cell60(5)791-801(1990 Mar)

cDNA sequences of chicken nucleolin/C23 and NO38/B23, two major nucleolar proteins.
Maridor G, Nigg EA
Nucleic acids research18(5)1286(1990 Mar)

Major nucleolar proteins shuttle between nucleus and cytoplasm.
Borer RA, Lehner CF, Eppenberger HM, Nigg EA
Cell56(3)379-90(1989 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa27054	NM_205265.1 Latest version!	Gallus gallus nucleolin (NCL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCL ( NM_205265.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990596.1 Gallus gallus nucleolin (NCL), mRNA.

Price & Availability↑

CloneID	OGa27054
Clone ID Related Accession (Same CDS sequence)	NM_205265.1
Accession Version	NM_205265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Gallus gallus(chicken)
Product	nucleolin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X17199.1. ##Evidence-Data-START## Transcript exon combination :: X17199.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGAAGC TCGCCAAGAC TCCCAAGAAT CAAATGAAAC AGAAAAAAAT GGCGCCTCCC 
CCCAAAAAGG TCGAGGAAAG CGAAGAGGAA GAGTCCTCCG ACTTAGAGGA AAGCAGCGGG 
GAAGAGGTGA TGGTCCCTCC AAAGAAACAA CAAAAAGCAG CAGTTACACC AGCCAAGAAG 
GCTGCTACCC CTGCAAAGAA GGCTGCTACT CCTGCAAAAA AGGCAGTGAC ACCAGCCAAG 
AAGGCTGTGG CTACTCCAGC TAAAAAGGCT GTTGCTCCAT CCCCCAAAAA GGCTGCTGTC 
GTAGGCAAAG GGGCAAAAAA TGGCAAGAAT GCCAAAAAGG AAGAGAGCGA GGAGGAAGAT 
GAAGACGATG AAGATGATGA GGAAGATGAG GATGAAGAAG AGGAGTCTGA TGAGGAAGAG 
GAACCAGCAG TGCCTGTGAA GCCTGCAGCC AAAAAGTCCG CAGCAGCTGT ACCAGCCAAA 
AAGCCTGCAG TTGTGCCAGC AAAGCAAGAA TCTGAGGAGG AGGAGGAGGA AGATGATGAA 
GAGGAGGACG AGGAAGATGA CGAGTCTGAA GATGAGGCCA TGGACACAAC TCCTGCTCCT 
GTGAAGAAAC CTACTCCAGC AAAGGCTACA CCAGCTAAAG CCAAGGCGGA GTCTGAAGAT 
GAGGAAGATG AGGAGGATGA GGATGAAGAT GAGGAGGATG AAGATGATGA AGAGGAAGAT 
GAGGAAGAAA GTGAGGATGA AAAACCTGTC AAGGAAGCAC CTGGAAAAAG GAAAAAAGAA 
ATGGCCAATA AGAGTGCACC AGAGGCCAAG AAAAAGAAAA CAGAAACACC TGCTTCAGCT 
TTCTCACTCT TCGTGAAAAA CTTGACCCCC ACCAAGGACT ATGAAGAACT GAGGACTGCC 
ATCAAAGAAT TCTTTGGCAA GAAAAATCTC CAAGTCTCAG AAGTCAGAAT CGGTTCTTCC 
AAGCGGTTTG GCTATGTGGA CTTCTTATCT GCTGAAGATA TGGATAAAGC TCTTCAACTG 
AATGGAAAGA AGCTGATGGG TTTGGAAATC AAACTGGAAA AAGCAAAGAG CAAAGAAAGT 
CTTAAAGAAA ATAAGAAAGA GAGAGATGCC AGAACACTGT TTGTGAAGAA TCTGCCCTAC 
CGTGTAACTG AAGATGAAAT GAAAAATGTA TTTGAAAACG CTTTAGAAGT CCGACTAGTA 
CTTAACAAGG AAGGGAGCAG CAAAGGGATG GCCTACATTG AATTCAAAAC AGAAGCTGAG 
GCAGAAAAAG CACTGGAGGA GAAGCAAGGC ACAGAGGTTG ACGGTCGTGC CATGGTCATT 
GACTACACCG GTGAGAAGAG CCAACAAGAA AGTCAGAAAG GAGGTGGAGA GAGGGAGTCA 
AAGACTCTGA TTGTGAACAA CCTGTCATAT GCTGCCTCAG AAGAAACTCT CCAGGAACTG 
TTTAAAAAAG CGACTTCCAT CAAGATGCCA CAGAACAACC AGGGCAGGCC TAAAGGGTAT 
GCATTTGTAG AATTTCCCAC AGCCGAGGAT GCCAAAGAGG CATTGAATTC CTGTAACAAC 
ACAGAAATTG AAGGCAGAGC AATCAGGCTG GAATTCAGTT CACCATCGTG GCAGAAAGGG 
AACATGAATG CAAGAGGAGG ATTTAACCAA CAAAGCAAAA CATTGTTTGT CAGAGGCCTT 
TCTGAGGACA CAACGGAGGA GACGCTAAGA GAATCATTTG AAGGCTCTAT AAGTGCTAGA 
ATAGTCACAG ATAGAGACAC TGGATCTTCT AAAGGGTTTG GTTTTGTGGA CTTCAGCTCC 
CCAGAAGATG CCAAAGCAGC TAAAGAAGCT ATGGAGGATG GAGAGATAGA CGGAAACAAA 
GTGACCCTTG ATTTTGCCAA ACCAAAGGGT GAATTTCAGC GTGGCGGCGG ATTTGGTGGT 
GGATTTGGTG GTCGTGGTGG CAGAGGAGGC AGAGGAGGAG GAAGAGGTGG ATTTGGTGGC 
AGAGGTGGTG GCAGAGGGTT TGGAGGTAGA GGAGGTGGCT TCCGAGGAGG CAGAGGAGGA 
GGTGGAGATC ACAAGCCACA AGGGAAGAAG ATCAAGTTTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990596.1
CDS	101..2185
Translation	MVKLAKTPKNQMKQKKMAPPPKKVEESEEEESSDLEESSGEEVMVPPKKQQKAAVTPAKKAATPAKKAATPAKKAVTPAKKAVATPAKKAVAPSPKKAAVVGKGAKNGKNAKKEESEEEDEDDEDDEEDEDEEEESDEEEEPAVPVKPAAKKSAAAVPAKKPAVVPAKQESEEEEEEDDEEEDEEDDESEDEAMDTTPAPVKKPTPAKATPAKAKAESEDEEDEEDEDEDEEDEDDEEEDEEESEDEKPVKEAPGKRKKEMANKSAPEAKKKKTETPASAFSLFVKNLTPTKDYEELRTAIKEFFGKKNLQVSEVRIGSSKRFGYVDFLSAEDMDKALQLNGKKLMGLEIKLEKAKSKESLKENKKERDARTLFVKNLPYRVTEDEMKNVFENALEVRLVLNKEGSSKGMAYIEFKTEAEAEKALEEKQGTEVDGRAMVIDYTGEKSQQESQKGGGERESKTLIVNNLSYAASEETLQELFKKATSIKMPQNNQGRPKGYAFVEFPTAEDAKEALNSCNNTEIEGRAIRLEFSSPSWQKGNMNARGGFNQQSKTLFVRGLSEDTTEETLRESFEGSISARIVTDRDTGSSKGFGFVDFSSPEDAKAAKEAMEDGEIDGNKVTLDFAKPKGEFQRGGGFGGGFGGRGGRGGRGGGRGGFGGRGGGRGFGGRGGGFRGGRGGGGDHKPQGKKIKFE

Target ORF information:

RefSeq Version	NM_205265.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nucleolin (NCL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205265.1

ORF Insert Sequence:

ATGGTGAAGC TCGCCAAGAC TCCCAAGAAT CAAATGAAAC AGAAAAAAAT GGCGCCTCCC 
CCCAAAAAGG TCGAGGAAAG CGAAGAGGAA GAGTCCTCCG ACTTAGAGGA AAGCAGCGGG 
GAAGAGGTGA TGGTCCCTCC AAAGAAACAA CAAAAAGCAG CAGTTACACC AGCCAAGAAG 
GCTGCTACCC CTGCAAAGAA GGCTGCTACT CCTGCAAAAA AGGCAGTGAC ACCAGCCAAG 
AAGGCTGTGG CTACTCCAGC TAAAAAGGCT GTTGCTCCAT CCCCCAAAAA GGCTGCTGTC 
GTAGGCAAAG GGGCAAAAAA TGGCAAGAAT GCCAAAAAGG AAGAGAGCGA GGAGGAAGAT 
GAAGACGATG AAGATGATGA GGAAGATGAG GATGAAGAAG AGGAGTCTGA TGAGGAAGAG 
GAACCAGCAG TGCCTGTGAA GCCTGCAGCC AAAAAGTCCG CAGCAGCTGT ACCAGCCAAA 
AAGCCTGCAG TTGTGCCAGC AAAGCAAGAA TCTGAGGAGG AGGAGGAGGA AGATGATGAA 
GAGGAGGACG AGGAAGATGA CGAGTCTGAA GATGAGGCCA TGGACACAAC TCCTGCTCCT 
GTGAAGAAAC CTACTCCAGC AAAGGCTACA CCAGCTAAAG CCAAGGCGGA GTCTGAAGAT 
GAGGAAGATG AGGAGGATGA GGATGAAGAT GAGGAGGATG AAGATGATGA AGAGGAAGAT 
GAGGAAGAAA GTGAGGATGA AAAACCTGTC AAGGAAGCAC CTGGAAAAAG GAAAAAAGAA 
ATGGCCAATA AGAGTGCACC AGAGGCCAAG AAAAAGAAAA CAGAAACACC TGCTTCAGCT 
TTCTCACTCT TCGTGAAAAA CTTGACCCCC ACCAAGGACT ATGAAGAACT GAGGACTGCC 
ATCAAAGAAT TCTTTGGCAA GAAAAATCTC CAAGTCTCAG AAGTCAGAAT CGGTTCTTCC 
AAGCGGTTTG GCTATGTGGA CTTCTTATCT GCTGAAGATA TGGATAAAGC TCTTCAACTG 
AATGGAAAGA AGCTGATGGG TTTGGAAATC AAACTGGAAA AAGCAAAGAG CAAAGAAAGT 
CTTAAAGAAA ATAAGAAAGA GAGAGATGCC AGAACACTGT TTGTGAAGAA TCTGCCCTAC 
CGTGTAACTG AAGATGAAAT GAAAAATGTA TTTGAAAACG CTTTAGAAGT CCGACTAGTA 
CTTAACAAGG AAGGGAGCAG CAAAGGGATG GCCTACATTG AATTCAAAAC AGAAGCTGAG 
GCAGAAAAAG CACTGGAGGA GAAGCAAGGC ACAGAGGTTG ACGGTCGTGC CATGGTCATT 
GACTACACCG GTGAGAAGAG CCAACAAGAA AGTCAGAAAG GAGGTGGAGA GAGGGAGTCA 
AAGACTCTGA TTGTGAACAA CCTGTCATAT GCTGCCTCAG AAGAAACTCT CCAGGAACTG 
TTTAAAAAAG CGACTTCCAT CAAGATGCCA CAGAACAACC AGGGCAGGCC TAAAGGGTAT 
GCATTTGTAG AATTTCCCAC AGCCGAGGAT GCCAAAGAGG CATTGAATTC CTGTAACAAC 
ACAGAAATTG AAGGCAGAGC AATCAGGCTG GAATTCAGTT CACCATCGTG GCAGAAAGGG 
AACATGAATG CAAGAGGAGG ATTTAACCAA CAAAGCAAAA CATTGTTTGT CAGAGGCCTT 
TCTGAGGACA CAACGGAGGA GACGCTAAGA GAATCATTTG AAGGCTCTAT AAGTGCTAGA 
ATAGTCACAG ATAGAGACAC TGGATCTTCT AAAGGGTTTG GTTTTGTGGA CTTCAGCTCC 
CCAGAAGATG CCAAAGCAGC TAAAGAAGCT ATGGAGGATG GAGAGATAGA CGGAAACAAA 
GTGACCCTTG ATTTTGCCAA ACCAAAGGGT GAATTTCAGC GTGGCGGCGG ATTTGGTGGT 
GGATTTGGTG GTCGTGGTGG CAGAGGAGGC AGAGGAGGAG GAAGAGGTGG ATTTGGTGGC 
AGAGGTGGTG GCAGAGGGTT TGGAGGTAGA GGAGGTGGCT TCCGAGGAGG CAGAGGAGGA 
GGTGGAGATC ACAAGCCACA AGGGAAGAAG ATCAAGTTTG AATAA