PASK cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	PASK
Entrez Gene ID	101096339
Full Name	PAS domain containing serine/threonine kinase
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019838992.2	XP_019694551.1	PAS domain-containing serine/threonine-protein kinase isoform X2
XM_006935747.2	XP_006935809.1	PAS domain-containing serine/threonine-protein kinase isoform X3
XM_019838992.1	XP_019694551.1	PAS domain-containing serine/threonine-protein kinase isoform X2
XM_006935745.3	XP_006935807.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006935746.3	XP_006935808.1	PAS domain-containing serine/threonine-protein kinase isoform X1
XM_006935747.3	XP_006935809.1	PAS domain-containing serine/threonine-protein kinase isoform X3
XM_006935745.2	XP_006935807.1	PAS domain-containing serine/threonine-protein kinase isoform X1

Pathways from BioSystems
Gene Ontology

The following PASK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PASK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb12790	XM_006935747.3 Latest version!	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb48032	XM_019838992.2 Latest version!	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb12789	XM_006935746.3 Latest version!	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb12789	XM_006935745.3 Latest version!	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OFb48032	XM_019838992.1	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb12790	XM_006935747.2	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb12789	XM_006935745.2	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PASK ( XM_006935747.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006935809.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb12790
Clone ID Related Accession (Same CDS sequence)	XM_006935747.2 , XM_006935747.3
Accession Version	XM_006935747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006935747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGA AGGCAGATGG AGCTCCTACT GTCTGTCCTC GCTGGCTGCC 
CAGAATATGT GCGCGAGCAA GCCTCACTGC CTGGCCGCAC CGGAGCGGTC GGACGCGGCC 
GGGTCCCTGG GGGGCGCGTC CTGCTGTTCC CTGCTGCGGG GCTTGTCTTT GGGGCGGTCC 
ACGCCCCTCC TCCCGGCCCC CGTGTCCAAC CCGAACAAGG CTGTTTTCAC CGTGGACGCC 
AAGACCACAG AGATCCTGGT CGCCAACGAC AAGGCTTGCC AGCTCCTAGG GTACAGCAGT 
CATGACCTGA TCGGCCAGAA GCTCACGCAG TTCTTCCTGA AGCCAGATTC CGACGTGGTG 
AAGGCCCTCA GCGAAGAGCA TGTGGAGGCT GACGGCCACG CCGCCGTTGT GTTTGGCACG 
GTGGTGGACA TCGTGAGCCG CAGTGGGGAG AAGGTTCCAG TTTCTGTGTG GATGAAGAAG 
GTGAAGCGGG AACGCAGCCT CTGCTGTGTG GTCATGCTGG AGCCCGTGGA GCGCGTCTCG 
GCCTGGGTCG CTTTCCAGAG CGATGGAACC ATAACGTCGT GCGACAGCCT CTTCCCTCTC 
CTGTACGGGT ACACGTCCGG GGAGGAGGTG CTCGGGCAGC ACATGGCAGA CCTCATCCCT 
TCTGTGCAGC TGCCTCCGCC CGGTGAGCTC GTCCCACAGA ATCTCAAGAT TCAGAGGTCT 
GTCGGGAGGG CCAAAGATGG CACCACTTTC CCTCTGAGCT TAAAACTGAA ATCCGAGCCC 
AGAAGCGAGA TGGTGGAAGA CGGCAGGGCA GTGCCTGAGC GGGGTTACTC GGCGTCAGTC 
TGGGTGTTCT CCACCATCAG CGGCCTCATC ACCCTCCTGC CCGACGGGAC CATCTATGGC 
ATCAACCACA GCTTTGCCCT GATGCTGTTC GGTTACGGGA AGACGGAGCT CCTGGGCAAG 
AATATCACTT TTCTGATTCC CGGTTTCTAC GACTACATGG AGCTCGCATA TGACAGTTCC 
TTACCACGGC CAAACCTGAC CAACTGCTTG GACGTTGGCA ACCAGAGTGA GCCTGGGGAG 
AGTGCTGTGG ACCCCCACCA GGGCTGGGAC CCGGCCGGCG AGGCCAAGGG TCGGCCCAGC 
CTCTTCCTCC CAGATTCCAG GGTTAACGGC TCGCTTGTCG GAGACCACGA GCTGCCACGA 
GGCGAGACCC TAGAGGTAGC GGGAAGCCGA GAAGCCTTCG CAGGGACTGG GGGCCAGGCA 
GACCCTGGAG GCCACCTCCC TCCTGCCCTC CCGCCTCTGC CTGTTCCACG AGGGGACAAC 
ATCCCAGAAG GAAGCCTGCC AGCCCAGGAA CGGTCATCGC CCAAGGACCA GCAGAACATC 
CCAGAAGGAT GCCCACCATC CCAGGAACAG TCATTGCCTG AGGACCGGCG GAACATCCCA 
GAAGGATGCT CACCATCCCA GGAACAGTCA TTGTCTGAGG GCCAGTGGAA CATCTCAGGA 
GGATGCCCAC CATCCCAGGA ACAGTCACTG CCTGAGGACC AGCAGAAGTT CCCAGAAGGA 
TGCCCACCAT CCCAGGAATA CTCATTGCCT GAGGACCAGC GGAACATCCC AGAAGGATGC 
CCACCATGCC AGGAACAGTC CTTGCCTGAG GATCAGCGGA ACATCCCAGA AGGAAGCTCA 
CTAGCCTTCA GTAAGTGGTT ATTTCCTGAG GGCCAGCAGC AGAACACTCC AGAAGGAAGC 
CTGGCAGCCC TCAGAGAAAA GTCATTGTCC AAGGACCAGC AGAACACCCT GGAAAGAAGC 
CCGACAACCC AGGAATACTC ATTGTCCAAG GACCAGCGGA ACCTCCCAGA AGGATGCCCG 
CCAGCGCATA GCCAGTGGTC CTTACCCGAG GCCCAGCAGG ACACTGTCTT GGAGAAGGAG 
GAGCGTGCAG CCCCAGAGAG CCTCATACGG GACCTTGAGG GAAAAAGTGG GTCTGGCCCC 
AGGGACACGT GGATGTTTGC TTCACGTGAA GACTCTGAAG GCCCGGCTCC AGCAGAGGGT 
GGGAGCAGAA ACCCTGGGGT GTGTGGCCCG CACCAGGAGG CACAGCTGGA GGGGGCAGGT 
GTGAGCAGTG CCGGTGGTTC AAACCATCGG GCTGGTTCTA TCATGCCCGT GCCCCATGCT 
GCAGGCCAGC CGGCGGGACA GCGCCTCCTG CCGTGCTACC CCAGGTATGG GGGTGAGCAG 
AACCCGCAGC CCGGAAGCCC CCCCAGGGCA GCGCCACCCT CATTCTGGAA GCCTGTGCTA 
GATGAGCCGT GGGCAGGGGA GAGTGACCGA GATGAGCTGC AGACTTGCCT AATTAAGGAG 
CGGCTGTCCA AGTGGAGCGT CGTGGGGCCA CCGGGCGTCT CTTACACAGA GCACCCCCTA 
TTCTCTGCCC CTGTGTCGCT GTGTGACCCG GGAGGTGGAG GCCCGCGCAG CCGTGTGGGC 
AGCTCCGCGG CTTACTACGC ACTGGCCACA GACCTCCCCG GGGTCCTGGA TGCGGTGCAG 
GCCCGGGAGG CCGATGGGAA TTCATTTTCC TGGAATCTCA AGGAACTCTT TTTCGGTGAA 
CGGACAGACC GAACGTTCTC GAACCGTTCC TGTACCGCGT TGGAGCTTGT AGAGATGGCC 
TCTCCTTCGC TGGCGGGCTC TGACGCAGAC CTGAGTGGTC CGCACGGGCC CGGGTCACAA 
GTTCTGGACG ACAGAGAGCT GTTGCTCCTG ACGGGCACCT GCTTGCATCT TGGTGAAGTT 
CAGCCTCGCG AGAGCTGTCT GGGGCGCGGC CCGGCCGAGC CCTCTGAGAC CCGTGTGGTG 
TCCTCAGGAC CCTACAAAGT GAGTGGCAGA GAGAACCCAA CCTGCGTCCT TCCCACGCCG 
GGAGCTGGCT CCGTGGAGGT GTGCCCGTTG GAGGAGCCCA GGCTGAACTT CCAGGTCACC 
TCCACGCCCG TGAAAGGGGA CAGCCCGGCG ATGCCCGGGG CCCCTCTGCA GCATGAGATC 
CAGGAGGGCA CCTATTCCGG CAGCTGTTAC CACCGAGACG GCTCGTGGCT GAGTGTGCAG 
TTTGAGGTGA AGCGGGTGGA GCTCCAGGGC TCCGCGACCC TGTTCTGCTG CTGGCTCGTG 
AAAGACCTGC TACGCAGCCA CCGGGACTCG GCCGCGCGGA CCCGCCTGCT CCTGGCCAGC 
CTGCCCAGCT CCAGCCACTC CATGTGCGAG CTTTCTGGAC CCAGCACAGG GGAGATGCTC 
AGGAGCAAGC CCTGGTTTGA GGAGCCTCCG ACGGCTCTGG AGGTGGAGGG GCTGGCCGCC 
TGCGAGGGCG CGTACTCCCG CAAGTACAGC ACGCTGAGCC CACTGGGCAG CGGGGCCTTC 
GGCTTCGTGT GGACCGCGGT GGACAGAGAG GAGAATAAGG AGGTGGTGGT GAAGTTTATT 
AAGAAGGAGA AGGTGTTGGA GGATTGTTGG ATTGAGGATC CCAAACTTGG AAAAGTTACT 
TTGGAGATTG CGATTCTGTC CAGGGTGGAG CACGCCAACA TCATCAAGGT GGTGGACGTC 
TTTGAAAACC AAGAGTTCTT TCAGCTGGTG ATGGAGAAGC ACGGCTCCGG CCTGGACCTC 
TTTGCGTTCA TCGACCGCCA CCCCGGCCTG GACGAGCCCC TGGCGAGCTA CATCTTCCGA 
CAGCTGGTGT CGGCAGTGGC CTACCTGCGC TCCAAGAGCA TCATTCACCG GGACATCAAG 
GACGAGAACA TCGTGATCGC GGAGGACTTT ACCATCAAGC TGATCGACTT CGGCTCGGCT 
GCCTACCTGG AGAAGGGCAG GCTGTTTCAC ACGTTCTGTG GGACCATCGA GTACTGTGCG 
CCCGAGGTGC TCCTGGGAAA CCCGTACAAG GGGCCGGAGC TGGAGATGTG GTCACTGGGG 
GTCACGCTGT ACACGCTGAT CTTTGAGGAG AACCCTTTCT GCGAGCTGGA GGAGACCATG 
GAGGCCGTGA TCCACCCGCC ATTCCTGGTG TCAGAAGATC TCATGAACCT CGTGTCTGGG 
CTGCTGCAGC CTATCCCCGA GCAGCGCACC ACCTTGGAGA AGCTGGTGAC AGACCCCTGG 
GTGACACAGC CTGTGAACCT TGCTGACTAC ACCTGGGATG AGGTGTGTCG AGTGAACAAG 
CCAGAAAGTG GAGGTCTGTC GGCGCTGAGT CTGGAGATGG AGAGCAGGTG CGCGAGGGAA 
GTGGCCCCAG AGCCATGCGG GGCCTTGTGC CCTAGGGGCG GCCCCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006935809.1
CDS	253..4623
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSEGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEMLRSKPWFEEPPTALEVEGLAACEGAYSRKYSTLSPLGSGAFGFVWTAVDREENKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_006935747.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006935747.2

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGA AGGCAGATGG AGCTCCTACT GTCTGTCCTC GCTGGCTGCC 
CAGAATATGT GCGCGAGCAA GCCTCACTGC CTGGCCGCAC CGGAGCGGTC GGACGCGGCC 
GGGTCCCTGG GGGGCGCGTC CTGCTGTTCC CTGCTGCGGG GCTTGTCTTT GGGGCGGTCC 
ACGCCCCTCC TCCCGGCCCC CGTGTCCAAC CCGAACAAGG CTGTTTTCAC CGTGGACGCC 
AAGACCACAG AGATCCTGGT CGCCAACGAC AAGGCTTGCC AGCTCCTAGG GTACAGCAGT 
CATGACCTGA TCGGCCAGAA GCTCACGCAG TTCTTCCTGA AGCCAGATTC CGACGTGGTG 
AAGGCCCTCA GCGAAGAGCA TGTGGAGGCT GACGGCCACG CCGCCGTTGT GTTTGGCACG 
GTGGTGGACA TCGTGAGCCG CAGTGGGGAG AAGGTTCCAG TTTCTGTGTG GATGAAGAAG 
GTGAAGCGGG AACGCAGCCT CTGCTGTGTG GTCATGCTGG AGCCCGTGGA GCGCGTCTCG 
GCCTGGGTCG CTTTCCAGAG CGATGGAACC ATAACGTCGT GCGACAGCCT CTTCCCTCTC 
CTGTACGGGT ACACGTCCGG GGAGGAGGTG CTCGGGCAGC ACATGGCAGA CCTCATCCCT 
TCTGTGCAGC TGCCTCCGCC CGGTGAGCTC GTCCCACAGA ATCTCAAGAT TCAGAGGTCT 
GTCGGGAGGG CCAAAGATGG CACCACTTTC CCTCTGAGCT TAAAACTGAA ATCCGAGCCC 
AGAAGCGAGA TGGTGGAAGA CGGCAGGGCA GTGCCTGAGC GGGGTTACTC GGCGTCAGTC 
TGGGTGTTCT CCACCATCAG CGGCCTCATC ACCCTCCTGC CCGACGGGAC CATCTATGGC 
ATCAACCACA GCTTTGCCCT GATGCTGTTC GGTTACGGGA AGACGGAGCT CCTGGGCAAG 
AATATCACTT TTCTGATTCC CGGTTTCTAC GACTACATGG AGCTCGCATA TGACAGTTCC 
TTACCACGGC CAAACCTGAC CAACTGCTTG GACGTTGGCA ACCAGAGTGA GCCTGGGGAG 
AGTGCTGTGG ACCCCCACCA GGGCTGGGAC CCGGCCGGCG AGGCCAAGGG TCGGCCCAGC 
CTCTTCCTCC CAGATTCCAG GGTTAACGGC TCGCTTGTCG GAGACCACGA GCTGCCACGA 
GGCGAGACCC TAGAGGTAGC GGGAAGCCGA GAAGCCTTCG CAGGGACTGG GGGCCAGGCA 
GACCCTGGAG GCCACCTCCC TCCTGCCCTC CCGCCTCTGC CTGTTCCACG AGGGGACAAC 
ATCCCAGAAG GAAGCCTGCC AGCCCAGGAA CGGTCATCGC CCAAGGACCA GCAGAACATC 
CCAGAAGGAT GCCCACCATC CCAGGAACAG TCATTGCCTG AGGACCGGCG GAACATCCCA 
GAAGGATGCT CACCATCCCA GGAACAGTCA TTGTCTGAGG GCCAGTGGAA CATCTCAGGA 
GGATGCCCAC CATCCCAGGA ACAGTCACTG CCTGAGGACC AGCAGAAGTT CCCAGAAGGA 
TGCCCACCAT CCCAGGAATA CTCATTGCCT GAGGACCAGC GGAACATCCC AGAAGGATGC 
CCACCATGCC AGGAACAGTC CTTGCCTGAG GATCAGCGGA ACATCCCAGA AGGAAGCTCA 
CTAGCCTTCA GTAAGTGGTT ATTTCCTGAG GGCCAGCAGC AGAACACTCC AGAAGGAAGC 
CTGGCAGCCC TCAGAGAAAA GTCATTGTCC AAGGACCAGC AGAACACCCT GGAAAGAAGC 
CCGACAACCC AGGAATACTC ATTGTCCAAG GACCAGCGGA ACCTCCCAGA AGGATGCCCG 
CCAGCGCATA GCCAGTGGTC CTTACCCGAG GCCCAGCAGG ACACTGTCTT GGAGAAGGAG 
GAGCGTGCAG CCCCAGAGAG CCTCATACGG GACCTTGAGG GAAAAAGTGG GTCTGGCCCC 
AGGGACACGT GGATGTTTGC TTCACGTGAA GACTCTGAAG GCCCGGCTCC AGCAGAGGGT 
GGGAGCAGAA ACCCTGGGGT GTGTGGCCCG CACCAGGAGG CACAGCTGGA GGGGGCAGGT 
GTGAGCAGTG CCGGTGGTTC AAACCATCGG GCTGGTTCTA TCATGCCCGT GCCCCATGCT 
GCAGGCCAGC CGGCGGGACA GCGCCTCCTG CCGTGCTACC CCAGGTATGG GGGTGAGCAG 
AACCCGCAGC CCGGAAGCCC CCCCAGGGCA GCGCCACCCT CATTCTGGAA GCCTGTGCTA 
GATGAGCCGT GGGCAGGGGA GAGTGACCGA GATGAGCTGC AGACTTGCCT AATTAAGGAG 
CGGCTGTCCA AGTGGAGCGT CGTGGGGCCA CCGGGCGTCT CTTACACAGA GCACCCCCTA 
TTCTCTGCCC CTGTGTCGCT GTGTGACCCG GGAGGTGGAG GCCCGCGCAG CCGTGTGGGC 
AGCTCCGCGG CTTACTACGC ACTGGCCACA GACCTCCCCG GGGTCCTGGA TGCGGTGCAG 
GCCCGGGAGG CCGATGGGAA TTCATTTTCC TGGAATCTCA AGGAACTCTT TTTCGGTGAA 
CGGACAGACC GAACGTTCTC GAACCGTTCC TGTACCGCGT TGGAGCTTGT AGAGATGGCC 
TCTCCTTCGC TGGCGGGCTC TGACGCAGAC CTGAGTGGTC CGCACGGGCC CGGGTCACAA 
GTTCTGGACG ACAGAGAGCT GTTGCTCCTG ACGGGCACCT GCTTGCATCT TGGTGAAGTT 
CAGCCTCGCG AGAGCTGTCT GGGGCGCGGC CCGGCCGAGC CCTCTGAGAC CCGTGTGGTG 
TCCTCAGGAC CCTACAAAGT GAGTGGCAGA GAGAACCCAA CCTGCGTCCT TCCCACGCCG 
GGAGCTGGCT CCGTGGAGGT GTGCCCGTTG GAGGAGCCCA GGCTGAACTT CCAGGTCACC 
TCCACGCCCG TGAAAGGGGA CAGCCCGGCG ATGCCCGGGG CCCCTCTGCA GCATGAGATC 
CAGGAGGGCA CCTATTCCGG CAGCTGTTAC CACCGAGACG GCTCGTGGCT GAGTGTGCAG 
TTTGAGGTGA AGCGGGTGGA GCTCCAGGGC TCCGCGACCC TGTTCTGCTG CTGGCTCGTG 
AAAGACCTGC TACGCAGCCA CCGGGACTCG GCCGCGCGGA CCCGCCTGCT CCTGGCCAGC 
CTGCCCAGCT CCAGCCACTC CATGTGCGAG CTTTCTGGAC CCAGCACAGG GGAGATGCTC 
AGGAGCAAGC CCTGGTTTGA GGAGCCTCCG ACGGCTCTGG AGGTGGAGGG GCTGGCCGCC 
TGCGAGGGCG CGTACTCCCG CAAGTACAGC ACGCTGAGCC CACTGGGCAG CGGGGCCTTC 
GGCTTCGTGT GGACCGCGGT GGACAGAGAG GAGAATAAGG AGGTGGTGGT GAAGTTTATT 
AAGAAGGAGA AGGTGTTGGA GGATTGTTGG ATTGAGGATC CCAAACTTGG AAAAGTTACT 
TTGGAGATTG CGATTCTGTC CAGGGTGGAG CACGCCAACA TCATCAAGGT GGTGGACGTC 
TTTGAAAACC AAGAGTTCTT TCAGCTGGTG ATGGAGAAGC ACGGCTCCGG CCTGGACCTC 
TTTGCGTTCA TCGACCGCCA CCCCGGCCTG GACGAGCCCC TGGCGAGCTA CATCTTCCGA 
CAGCTGGTGT CGGCAGTGGC CTACCTGCGC TCCAAGAGCA TCATTCACCG GGACATCAAG 
GACGAGAACA TCGTGATCGC GGAGGACTTT ACCATCAAGC TGATCGACTT CGGCTCGGCT 
GCCTACCTGG AGAAGGGCAG GCTGTTTCAC ACGTTCTGTG GGACCATCGA GTACTGTGCG 
CCCGAGGTGC TCCTGGGAAA CCCGTACAAG GGGCCGGAGC TGGAGATGTG GTCACTGGGG 
GTCACGCTGT ACACGCTGAT CTTTGAGGAG AACCCTTTCT GCGAGCTGGA GGAGACCATG 
GAGGCCGTGA TCCACCCGCC ATTCCTGGTG TCAGAAGATC TCATGAACCT CGTGTCTGGG 
CTGCTGCAGC CTATCCCCGA GCAGCGCACC ACCTTGGAGA AGCTGGTGAC AGACCCCTGG 
GTGACACAGC CTGTGAACCT TGCTGACTAC ACCTGGGATG AGGTGTGTCG AGTGAACAAG 
CCAGAAAGTG GAGGTCTGTC GGCGCTGAGT CTGGAGATGG AGAGCAGGTG CGCGAGGGAA 
GTGGCCCCAG AGCCATGCGG GGCCTTGTGC CCTAGGGGCG GCCCCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_006935747.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006935809.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OFb12790
Clone ID Related Accession (Same CDS sequence)	XM_006935747.2 , XM_006935747.3
Accession Version	XM_006935747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_006935747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGA AGGCAGATGG AGCTCCTACT GTCTGTCCTC GCTGGCTGCC 
CAGAATATGT GCGCGAGCAA GCCTCACTGC CTGGCCGCAC CGGAGCGGTC GGACGCGGCC 
GGGTCCCTGG GGGGCGCGTC CTGCTGTTCC CTGCTGCGGG GCTTGTCTTT GGGGCGGTCC 
ACGCCCCTCC TCCCGGCCCC CGTGTCCAAC CCGAACAAGG CTGTTTTCAC CGTGGACGCC 
AAGACCACAG AGATCCTGGT CGCCAACGAC AAGGCTTGCC AGCTCCTAGG GTACAGCAGT 
CATGACCTGA TCGGCCAGAA GCTCACGCAG TTCTTCCTGA AGCCAGATTC CGACGTGGTG 
AAGGCCCTCA GCGAAGAGCA TGTGGAGGCT GACGGCCACG CCGCCGTTGT GTTTGGCACG 
GTGGTGGACA TCGTGAGCCG CAGTGGGGAG AAGGTTCCAG TTTCTGTGTG GATGAAGAAG 
GTGAAGCGGG AACGCAGCCT CTGCTGTGTG GTCATGCTGG AGCCCGTGGA GCGCGTCTCG 
GCCTGGGTCG CTTTCCAGAG CGATGGAACC ATAACGTCGT GCGACAGCCT CTTCCCTCTC 
CTGTACGGGT ACACGTCCGG GGAGGAGGTG CTCGGGCAGC ACATGGCAGA CCTCATCCCT 
TCTGTGCAGC TGCCTCCGCC CGGTGAGCTC GTCCCACAGA ATCTCAAGAT TCAGAGGTCT 
GTCGGGAGGG CCAAAGATGG CACCACTTTC CCTCTGAGCT TAAAACTGAA ATCCGAGCCC 
AGAAGCGAGA TGGTGGAAGA CGGCAGGGCA GTGCCTGAGC GGGGTTACTC GGCGTCAGTC 
TGGGTGTTCT CCACCATCAG CGGCCTCATC ACCCTCCTGC CCGACGGGAC CATCTATGGC 
ATCAACCACA GCTTTGCCCT GATGCTGTTC GGTTACGGGA AGACGGAGCT CCTGGGCAAG 
AATATCACTT TTCTGATTCC CGGTTTCTAC GACTACATGG AGCTCGCATA TGACAGTTCC 
TTACCACGGC CAAACCTGAC CAACTGCTTG GACGTTGGCA ACCAGAGTGA GCCTGGGGAG 
AGTGCTGTGG ACCCCCACCA GGGCTGGGAC CCGGCCGGCG AGGCCAAGGG TCGGCCCAGC 
CTCTTCCTCC CAGATTCCAG GGTTAACGGC TCGCTTGTCG GAGACCACGA GCTGCCACGA 
GGCGAGACCC TAGAGGTAGC GGGAAGCCGA GAAGCCTTCG CAGGGACTGG GGGCCAGGCA 
GACCCTGGAG GCCACCTCCC TCCTGCCCTC CCGCCTCTGC CTGTTCCACG AGGGGACAAC 
ATCCCAGAAG GAAGCCTGCC AGCCCAGGAA CGGTCATCGC CCAAGGACCA GCAGAACATC 
CCAGAAGGAT GCCCACCATC CCAGGAACAG TCATTGCCTG AGGACCGGCG GAACATCCCA 
GAAGGATGCT CACCATCCCA GGAACAGTCA TTGTCTGAGG GCCAGTGGAA CATCTCAGGA 
GGATGCCCAC CATCCCAGGA ACAGTCACTG CCTGAGGACC AGCAGAAGTT CCCAGAAGGA 
TGCCCACCAT CCCAGGAATA CTCATTGCCT GAGGACCAGC GGAACATCCC AGAAGGATGC 
CCACCATGCC AGGAACAGTC CTTGCCTGAG GATCAGCGGA ACATCCCAGA AGGAAGCTCA 
CTAGCCTTCA GTAAGTGGTT ATTTCCTGAG GGCCAGCAGC AGAACACTCC AGAAGGAAGC 
CTGGCAGCCC TCAGAGAAAA GTCATTGTCC AAGGACCAGC AGAACACCCT GGAAAGAAGC 
CCGACAACCC AGGAATACTC ATTGTCCAAG GACCAGCGGA ACCTCCCAGA AGGATGCCCG 
CCAGCGCATA GCCAGTGGTC CTTACCCGAG GCCCAGCAGG ACACTGTCTT GGAGAAGGAG 
GAGCGTGCAG CCCCAGAGAG CCTCATACGG GACCTTGAGG GAAAAAGTGG GTCTGGCCCC 
AGGGACACGT GGATGTTTGC TTCACGTGAA GACTCTGAAG GCCCGGCTCC AGCAGAGGGT 
GGGAGCAGAA ACCCTGGGGT GTGTGGCCCG CACCAGGAGG CACAGCTGGA GGGGGCAGGT 
GTGAGCAGTG CCGGTGGTTC AAACCATCGG GCTGGTTCTA TCATGCCCGT GCCCCATGCT 
GCAGGCCAGC CGGCGGGACA GCGCCTCCTG CCGTGCTACC CCAGGTATGG GGGTGAGCAG 
AACCCGCAGC CCGGAAGCCC CCCCAGGGCA GCGCCACCCT CATTCTGGAA GCCTGTGCTA 
GATGAGCCGT GGGCAGGGGA GAGTGACCGA GATGAGCTGC AGACTTGCCT AATTAAGGAG 
CGGCTGTCCA AGTGGAGCGT CGTGGGGCCA CCGGGCGTCT CTTACACAGA GCACCCCCTA 
TTCTCTGCCC CTGTGTCGCT GTGTGACCCG GGAGGTGGAG GCCCGCGCAG CCGTGTGGGC 
AGCTCCGCGG CTTACTACGC ACTGGCCACA GACCTCCCCG GGGTCCTGGA TGCGGTGCAG 
GCCCGGGAGG CCGATGGGAA TTCATTTTCC TGGAATCTCA AGGAACTCTT TTTCGGTGAA 
CGGACAGACC GAACGTTCTC GAACCGTTCC TGTACCGCGT TGGAGCTTGT AGAGATGGCC 
TCTCCTTCGC TGGCGGGCTC TGACGCAGAC CTGAGTGGTC CGCACGGGCC CGGGTCACAA 
GTTCTGGACG ACAGAGAGCT GTTGCTCCTG ACGGGCACCT GCTTGCATCT TGGTGAAGTT 
CAGCCTCGCG AGAGCTGTCT GGGGCGCGGC CCGGCCGAGC CCTCTGAGAC CCGTGTGGTG 
TCCTCAGGAC CCTACAAAGT GAGTGGCAGA GAGAACCCAA CCTGCGTCCT TCCCACGCCG 
GGAGCTGGCT CCGTGGAGGT GTGCCCGTTG GAGGAGCCCA GGCTGAACTT CCAGGTCACC 
TCCACGCCCG TGAAAGGGGA CAGCCCGGCG ATGCCCGGGG CCCCTCTGCA GCATGAGATC 
CAGGAGGGCA CCTATTCCGG CAGCTGTTAC CACCGAGACG GCTCGTGGCT GAGTGTGCAG 
TTTGAGGTGA AGCGGGTGGA GCTCCAGGGC TCCGCGACCC TGTTCTGCTG CTGGCTCGTG 
AAAGACCTGC TACGCAGCCA CCGGGACTCG GCCGCGCGGA CCCGCCTGCT CCTGGCCAGC 
CTGCCCAGCT CCAGCCACTC CATGTGCGAG CTTTCTGGAC CCAGCACAGG GGAGATGCTC 
AGGAGCAAGC CCTGGTTTGA GGAGCCTCCG ACGGCTCTGG AGGTGGAGGG GCTGGCCGCC 
TGCGAGGGCG CGTACTCCCG CAAGTACAGC ACGCTGAGCC CACTGGGCAG CGGGGCCTTC 
GGCTTCGTGT GGACCGCGGT GGACAGAGAG GAGAATAAGG AGGTGGTGGT GAAGTTTATT 
AAGAAGGAGA AGGTGTTGGA GGATTGTTGG ATTGAGGATC CCAAACTTGG AAAAGTTACT 
TTGGAGATTG CGATTCTGTC CAGGGTGGAG CACGCCAACA TCATCAAGGT GGTGGACGTC 
TTTGAAAACC AAGAGTTCTT TCAGCTGGTG ATGGAGAAGC ACGGCTCCGG CCTGGACCTC 
TTTGCGTTCA TCGACCGCCA CCCCGGCCTG GACGAGCCCC TGGCGAGCTA CATCTTCCGA 
CAGCTGGTGT CGGCAGTGGC CTACCTGCGC TCCAAGAGCA TCATTCACCG GGACATCAAG 
GACGAGAACA TCGTGATCGC GGAGGACTTT ACCATCAAGC TGATCGACTT CGGCTCGGCT 
GCCTACCTGG AGAAGGGCAG GCTGTTTCAC ACGTTCTGTG GGACCATCGA GTACTGTGCG 
CCCGAGGTGC TCCTGGGAAA CCCGTACAAG GGGCCGGAGC TGGAGATGTG GTCACTGGGG 
GTCACGCTGT ACACGCTGAT CTTTGAGGAG AACCCTTTCT GCGAGCTGGA GGAGACCATG 
GAGGCCGTGA TCCACCCGCC ATTCCTGGTG TCAGAAGATC TCATGAACCT CGTGTCTGGG 
CTGCTGCAGC CTATCCCCGA GCAGCGCACC ACCTTGGAGA AGCTGGTGAC AGACCCCTGG 
GTGACACAGC CTGTGAACCT TGCTGACTAC ACCTGGGATG AGGTGTGTCG AGTGAACAAG 
CCAGAAAGTG GAGGTCTGTC GGCGCTGAGT CTGGAGATGG AGAGCAGGTG CGCGAGGGAA 
GTGGCCCCAG AGCCATGCGG GGCCTTGTGC CCTAGGGGCG GCCCCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006935809.1
CDS	445..4815
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSEGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEMLRSKPWFEEPPTALEVEGLAACEGAYSRKYSTLSPLGSGAFGFVWTAVDREENKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_006935747.3
Organism	Felis catus(domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006935747.3

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGA AGGCAGATGG AGCTCCTACT GTCTGTCCTC GCTGGCTGCC 
CAGAATATGT GCGCGAGCAA GCCTCACTGC CTGGCCGCAC CGGAGCGGTC GGACGCGGCC 
GGGTCCCTGG GGGGCGCGTC CTGCTGTTCC CTGCTGCGGG GCTTGTCTTT GGGGCGGTCC 
ACGCCCCTCC TCCCGGCCCC CGTGTCCAAC CCGAACAAGG CTGTTTTCAC CGTGGACGCC 
AAGACCACAG AGATCCTGGT CGCCAACGAC AAGGCTTGCC AGCTCCTAGG GTACAGCAGT 
CATGACCTGA TCGGCCAGAA GCTCACGCAG TTCTTCCTGA AGCCAGATTC CGACGTGGTG 
AAGGCCCTCA GCGAAGAGCA TGTGGAGGCT GACGGCCACG CCGCCGTTGT GTTTGGCACG 
GTGGTGGACA TCGTGAGCCG CAGTGGGGAG AAGGTTCCAG TTTCTGTGTG GATGAAGAAG 
GTGAAGCGGG AACGCAGCCT CTGCTGTGTG GTCATGCTGG AGCCCGTGGA GCGCGTCTCG 
GCCTGGGTCG CTTTCCAGAG CGATGGAACC ATAACGTCGT GCGACAGCCT CTTCCCTCTC 
CTGTACGGGT ACACGTCCGG GGAGGAGGTG CTCGGGCAGC ACATGGCAGA CCTCATCCCT 
TCTGTGCAGC TGCCTCCGCC CGGTGAGCTC GTCCCACAGA ATCTCAAGAT TCAGAGGTCT 
GTCGGGAGGG CCAAAGATGG CACCACTTTC CCTCTGAGCT TAAAACTGAA ATCCGAGCCC 
AGAAGCGAGA TGGTGGAAGA CGGCAGGGCA GTGCCTGAGC GGGGTTACTC GGCGTCAGTC 
TGGGTGTTCT CCACCATCAG CGGCCTCATC ACCCTCCTGC CCGACGGGAC CATCTATGGC 
ATCAACCACA GCTTTGCCCT GATGCTGTTC GGTTACGGGA AGACGGAGCT CCTGGGCAAG 
AATATCACTT TTCTGATTCC CGGTTTCTAC GACTACATGG AGCTCGCATA TGACAGTTCC 
TTACCACGGC CAAACCTGAC CAACTGCTTG GACGTTGGCA ACCAGAGTGA GCCTGGGGAG 
AGTGCTGTGG ACCCCCACCA GGGCTGGGAC CCGGCCGGCG AGGCCAAGGG TCGGCCCAGC 
CTCTTCCTCC CAGATTCCAG GGTTAACGGC TCGCTTGTCG GAGACCACGA GCTGCCACGA 
GGCGAGACCC TAGAGGTAGC GGGAAGCCGA GAAGCCTTCG CAGGGACTGG GGGCCAGGCA 
GACCCTGGAG GCCACCTCCC TCCTGCCCTC CCGCCTCTGC CTGTTCCACG AGGGGACAAC 
ATCCCAGAAG GAAGCCTGCC AGCCCAGGAA CGGTCATCGC CCAAGGACCA GCAGAACATC 
CCAGAAGGAT GCCCACCATC CCAGGAACAG TCATTGCCTG AGGACCGGCG GAACATCCCA 
GAAGGATGCT CACCATCCCA GGAACAGTCA TTGTCTGAGG GCCAGTGGAA CATCTCAGGA 
GGATGCCCAC CATCCCAGGA ACAGTCACTG CCTGAGGACC AGCAGAAGTT CCCAGAAGGA 
TGCCCACCAT CCCAGGAATA CTCATTGCCT GAGGACCAGC GGAACATCCC AGAAGGATGC 
CCACCATGCC AGGAACAGTC CTTGCCTGAG GATCAGCGGA ACATCCCAGA AGGAAGCTCA 
CTAGCCTTCA GTAAGTGGTT ATTTCCTGAG GGCCAGCAGC AGAACACTCC AGAAGGAAGC 
CTGGCAGCCC TCAGAGAAAA GTCATTGTCC AAGGACCAGC AGAACACCCT GGAAAGAAGC 
CCGACAACCC AGGAATACTC ATTGTCCAAG GACCAGCGGA ACCTCCCAGA AGGATGCCCG 
CCAGCGCATA GCCAGTGGTC CTTACCCGAG GCCCAGCAGG ACACTGTCTT GGAGAAGGAG 
GAGCGTGCAG CCCCAGAGAG CCTCATACGG GACCTTGAGG GAAAAAGTGG GTCTGGCCCC 
AGGGACACGT GGATGTTTGC TTCACGTGAA GACTCTGAAG GCCCGGCTCC AGCAGAGGGT 
GGGAGCAGAA ACCCTGGGGT GTGTGGCCCG CACCAGGAGG CACAGCTGGA GGGGGCAGGT 
GTGAGCAGTG CCGGTGGTTC AAACCATCGG GCTGGTTCTA TCATGCCCGT GCCCCATGCT 
GCAGGCCAGC CGGCGGGACA GCGCCTCCTG CCGTGCTACC CCAGGTATGG GGGTGAGCAG 
AACCCGCAGC CCGGAAGCCC CCCCAGGGCA GCGCCACCCT CATTCTGGAA GCCTGTGCTA 
GATGAGCCGT GGGCAGGGGA GAGTGACCGA GATGAGCTGC AGACTTGCCT AATTAAGGAG 
CGGCTGTCCA AGTGGAGCGT CGTGGGGCCA CCGGGCGTCT CTTACACAGA GCACCCCCTA 
TTCTCTGCCC CTGTGTCGCT GTGTGACCCG GGAGGTGGAG GCCCGCGCAG CCGTGTGGGC 
AGCTCCGCGG CTTACTACGC ACTGGCCACA GACCTCCCCG GGGTCCTGGA TGCGGTGCAG 
GCCCGGGAGG CCGATGGGAA TTCATTTTCC TGGAATCTCA AGGAACTCTT TTTCGGTGAA 
CGGACAGACC GAACGTTCTC GAACCGTTCC TGTACCGCGT TGGAGCTTGT AGAGATGGCC 
TCTCCTTCGC TGGCGGGCTC TGACGCAGAC CTGAGTGGTC CGCACGGGCC CGGGTCACAA 
GTTCTGGACG ACAGAGAGCT GTTGCTCCTG ACGGGCACCT GCTTGCATCT TGGTGAAGTT 
CAGCCTCGCG AGAGCTGTCT GGGGCGCGGC CCGGCCGAGC CCTCTGAGAC CCGTGTGGTG 
TCCTCAGGAC CCTACAAAGT GAGTGGCAGA GAGAACCCAA CCTGCGTCCT TCCCACGCCG 
GGAGCTGGCT CCGTGGAGGT GTGCCCGTTG GAGGAGCCCA GGCTGAACTT CCAGGTCACC 
TCCACGCCCG TGAAAGGGGA CAGCCCGGCG ATGCCCGGGG CCCCTCTGCA GCATGAGATC 
CAGGAGGGCA CCTATTCCGG CAGCTGTTAC CACCGAGACG GCTCGTGGCT GAGTGTGCAG 
TTTGAGGTGA AGCGGGTGGA GCTCCAGGGC TCCGCGACCC TGTTCTGCTG CTGGCTCGTG 
AAAGACCTGC TACGCAGCCA CCGGGACTCG GCCGCGCGGA CCCGCCTGCT CCTGGCCAGC 
CTGCCCAGCT CCAGCCACTC CATGTGCGAG CTTTCTGGAC CCAGCACAGG GGAGATGCTC 
AGGAGCAAGC CCTGGTTTGA GGAGCCTCCG ACGGCTCTGG AGGTGGAGGG GCTGGCCGCC 
TGCGAGGGCG CGTACTCCCG CAAGTACAGC ACGCTGAGCC CACTGGGCAG CGGGGCCTTC 
GGCTTCGTGT GGACCGCGGT GGACAGAGAG GAGAATAAGG AGGTGGTGGT GAAGTTTATT 
AAGAAGGAGA AGGTGTTGGA GGATTGTTGG ATTGAGGATC CCAAACTTGG AAAAGTTACT 
TTGGAGATTG CGATTCTGTC CAGGGTGGAG CACGCCAACA TCATCAAGGT GGTGGACGTC 
TTTGAAAACC AAGAGTTCTT TCAGCTGGTG ATGGAGAAGC ACGGCTCCGG CCTGGACCTC 
TTTGCGTTCA TCGACCGCCA CCCCGGCCTG GACGAGCCCC TGGCGAGCTA CATCTTCCGA 
CAGCTGGTGT CGGCAGTGGC CTACCTGCGC TCCAAGAGCA TCATTCACCG GGACATCAAG 
GACGAGAACA TCGTGATCGC GGAGGACTTT ACCATCAAGC TGATCGACTT CGGCTCGGCT 
GCCTACCTGG AGAAGGGCAG GCTGTTTCAC ACGTTCTGTG GGACCATCGA GTACTGTGCG 
CCCGAGGTGC TCCTGGGAAA CCCGTACAAG GGGCCGGAGC TGGAGATGTG GTCACTGGGG 
GTCACGCTGT ACACGCTGAT CTTTGAGGAG AACCCTTTCT GCGAGCTGGA GGAGACCATG 
GAGGCCGTGA TCCACCCGCC ATTCCTGGTG TCAGAAGATC TCATGAACCT CGTGTCTGGG 
CTGCTGCAGC CTATCCCCGA GCAGCGCACC ACCTTGGAGA AGCTGGTGAC AGACCCCTGG 
GTGACACAGC CTGTGAACCT TGCTGACTAC ACCTGGGATG AGGTGTGTCG AGTGAACAAG 
CCAGAAAGTG GAGGTCTGTC GGCGCTGAGT CTGGAGATGG AGAGCAGGTG CGCGAGGGAA 
GTGGCCCCAG AGCCATGCGG GGCCTTGTGC CCTAGGGGCG GCCCCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_019838992.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019694551.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.

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CloneID	OFb48032
Clone ID Related Accession (Same CDS sequence)	XM_019838992.2 , XM_019838992.1
Accession Version	XM_019838992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019838992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGGTGGTGG TGAAGTTTAT TAAGAAGGAG 
AAGGTGTTGG AGGATTGTTG GATTGAGGAT CCCAAACTTG GAAAAGTTAC TTTGGAGATT 
GCGATTCTGT CCAGGGTGGA GCACGCCAAC ATCATCAAGG TGGTGGACGT CTTTGAAAAC 
CAAGAGTTCT TTCAGCTGGT GATGGAGAAG CACGGCTCCG GCCTGGACCT CTTTGCGTTC 
ATCGACCGCC ACCCCGGCCT GGACGAGCCC CTGGCGAGCT ACATCTTCCG ACAGCTGGTG 
TCGGCAGTGG CCTACCTGCG CTCCAAGAGC ATCATTCACC GGGACATCAA GGACGAGAAC 
ATCGTGATCG CGGAGGACTT TACCATCAAG CTGATCGACT TCGGCTCGGC TGCCTACCTG 
GAGAAGGGCA GGCTGTTTCA CACGTTCTGT GGGACCATCG AGTACTGTGC GCCCGAGGTG 
CTCCTGGGAA ACCCGTACAA GGGGCCGGAG CTGGAGATGT GGTCACTGGG GGTCACGCTG 
TACACGCTGA TCTTTGAGGA GAACCCTTTC TGCGAGCTGG AGGAGACCAT GGAGGCCGTG 
ATCCACCCGC CATTCCTGGT GTCAGAAGAT CTCATGAACC TCGTGTCTGG GCTGCTGCAG 
CCTATCCCCG AGCAGCGCAC CACCTTGGAG AAGCTGGTGA CAGACCCCTG GGTGACACAG 
CCTGTGAACC TTGCTGACTA CACCTGGGAT GAGGTGTGTC GAGTGAACAA GCCAGAAAGT 
GGAGGTCTGT CGGCGCTGAG TCTGGAGATG GAGAGCAGGT GCGCGAGGGA AGTGGCCCCA 
GAGCCATGCG GGGCCTTGTG CCCTAGGGGC GGCCCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019694551.1
CDS	445..4866
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSGTRPFWASWVAVSKTQARGRSGLWPLCLFLLRGRALCAGGGAGRSLSCAAVILRHEPVLRSVCERDPGLGCLAKGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_019838992.2
Organism	Felis catus(domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019838992.2

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGGTGGTGG TGAAGTTTAT TAAGAAGGAG 
AAGGTGTTGG AGGATTGTTG GATTGAGGAT CCCAAACTTG GAAAAGTTAC TTTGGAGATT 
GCGATTCTGT CCAGGGTGGA GCACGCCAAC ATCATCAAGG TGGTGGACGT CTTTGAAAAC 
CAAGAGTTCT TTCAGCTGGT GATGGAGAAG CACGGCTCCG GCCTGGACCT CTTTGCGTTC 
ATCGACCGCC ACCCCGGCCT GGACGAGCCC CTGGCGAGCT ACATCTTCCG ACAGCTGGTG 
TCGGCAGTGG CCTACCTGCG CTCCAAGAGC ATCATTCACC GGGACATCAA GGACGAGAAC 
ATCGTGATCG CGGAGGACTT TACCATCAAG CTGATCGACT TCGGCTCGGC TGCCTACCTG 
GAGAAGGGCA GGCTGTTTCA CACGTTCTGT GGGACCATCG AGTACTGTGC GCCCGAGGTG 
CTCCTGGGAA ACCCGTACAA GGGGCCGGAG CTGGAGATGT GGTCACTGGG GGTCACGCTG 
TACACGCTGA TCTTTGAGGA GAACCCTTTC TGCGAGCTGG AGGAGACCAT GGAGGCCGTG 
ATCCACCCGC CATTCCTGGT GTCAGAAGAT CTCATGAACC TCGTGTCTGG GCTGCTGCAG 
CCTATCCCCG AGCAGCGCAC CACCTTGGAG AAGCTGGTGA CAGACCCCTG GGTGACACAG 
CCTGTGAACC TTGCTGACTA CACCTGGGAT GAGGTGTGTC GAGTGAACAA GCCAGAAAGT 
GGAGGTCTGT CGGCGCTGAG TCTGGAGATG GAGAGCAGGT GCGCGAGGGA AGTGGCCCCA 
GAGCCATGCG GGGCCTTGTG CCCTAGGGGC GGCCCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_019838992.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019694551.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.

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CloneID	OFb48032
Clone ID Related Accession (Same CDS sequence)	XM_019838992.2 , XM_019838992.1
Accession Version	XM_019838992.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGGTGGTGG TGAAGTTTAT TAAGAAGGAG 
AAGGTGTTGG AGGATTGTTG GATTGAGGAT CCCAAACTTG GAAAAGTTAC TTTGGAGATT 
GCGATTCTGT CCAGGGTGGA GCACGCCAAC ATCATCAAGG TGGTGGACGT CTTTGAAAAC 
CAAGAGTTCT TTCAGCTGGT GATGGAGAAG CACGGCTCCG GCCTGGACCT CTTTGCGTTC 
ATCGACCGCC ACCCCGGCCT GGACGAGCCC CTGGCGAGCT ACATCTTCCG ACAGCTGGTG 
TCGGCAGTGG CCTACCTGCG CTCCAAGAGC ATCATTCACC GGGACATCAA GGACGAGAAC 
ATCGTGATCG CGGAGGACTT TACCATCAAG CTGATCGACT TCGGCTCGGC TGCCTACCTG 
GAGAAGGGCA GGCTGTTTCA CACGTTCTGT GGGACCATCG AGTACTGTGC GCCCGAGGTG 
CTCCTGGGAA ACCCGTACAA GGGGCCGGAG CTGGAGATGT GGTCACTGGG GGTCACGCTG 
TACACGCTGA TCTTTGAGGA GAACCCTTTC TGCGAGCTGG AGGAGACCAT GGAGGCCGTG 
ATCCACCCGC CATTCCTGGT GTCAGAAGAT CTCATGAACC TCGTGTCTGG GCTGCTGCAG 
CCTATCCCCG AGCAGCGCAC CACCTTGGAG AAGCTGGTGA CAGACCCCTG GGTGACACAG 
CCTGTGAACC TTGCTGACTA CACCTGGGAT GAGGTGTGTC GAGTGAACAA GCCAGAAAGT 
GGAGGTCTGT CGGCGCTGAG TCTGGAGATG GAGAGCAGGT GCGCGAGGGA AGTGGCCCCA 
GAGCCATGCG GGGCCTTGTG CCCTAGGGGC GGCCCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019694551.1
CDS	939..5360
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSGTRPFWASWVAVSKTQARGRSGLWPLCLFLLRGRALCAGGGAGRSLSCAAVILRHEPVLRSVCERDPGLGCLAKGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_019838992.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019838992.1

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGGTGGTGG TGAAGTTTAT TAAGAAGGAG 
AAGGTGTTGG AGGATTGTTG GATTGAGGAT CCCAAACTTG GAAAAGTTAC TTTGGAGATT 
GCGATTCTGT CCAGGGTGGA GCACGCCAAC ATCATCAAGG TGGTGGACGT CTTTGAAAAC 
CAAGAGTTCT TTCAGCTGGT GATGGAGAAG CACGGCTCCG GCCTGGACCT CTTTGCGTTC 
ATCGACCGCC ACCCCGGCCT GGACGAGCCC CTGGCGAGCT ACATCTTCCG ACAGCTGGTG 
TCGGCAGTGG CCTACCTGCG CTCCAAGAGC ATCATTCACC GGGACATCAA GGACGAGAAC 
ATCGTGATCG CGGAGGACTT TACCATCAAG CTGATCGACT TCGGCTCGGC TGCCTACCTG 
GAGAAGGGCA GGCTGTTTCA CACGTTCTGT GGGACCATCG AGTACTGTGC GCCCGAGGTG 
CTCCTGGGAA ACCCGTACAA GGGGCCGGAG CTGGAGATGT GGTCACTGGG GGTCACGCTG 
TACACGCTGA TCTTTGAGGA GAACCCTTTC TGCGAGCTGG AGGAGACCAT GGAGGCCGTG 
ATCCACCCGC CATTCCTGGT GTCAGAAGAT CTCATGAACC TCGTGTCTGG GCTGCTGCAG 
CCTATCCCCG AGCAGCGCAC CACCTTGGAG AAGCTGGTGA CAGACCCCTG GGTGACACAG 
CCTGTGAACC TTGCTGACTA CACCTGGGAT GAGGTGTGTC GAGTGAACAA GCCAGAAAGT 
GGAGGTCTGT CGGCGCTGAG TCTGGAGATG GAGAGCAGGT GCGCGAGGGA AGTGGCCCCA 
GAGCCATGCG GGGCCTTGTG CCCTAGGGGC GGCCCCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_006935745.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006935807.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb12789
Clone ID Related Accession (Same CDS sequence)	XM_006935745.3 , XM_006935746.3 , XM_006935745.2
Accession Version	XM_006935745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_006935745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006935807.1
CDS	445..5034
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSGTRPFWASWVAVSKTQARGRSGLWPLCLFLLRGRALCAGGGAGRSLSCAAVILRHEPVLRSVCERDPGLGCLAKGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEMLRSKPWFEEPPTALEVEGLAACEGAYSRKYSTLSPLGSGAFGFVWTAVDREENKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_006935745.3
Organism	Felis catus(domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006935745.3

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_006935746.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006935808.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb12789
Clone ID Related Accession (Same CDS sequence)	XM_006935745.3 , XM_006935746.3 , XM_006935745.2
Accession Version	XM_006935746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006935746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006935808.1
CDS	196..4785
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSGTRPFWASWVAVSKTQARGRSGLWPLCLFLLRGRALCAGGGAGRSLSCAAVILRHEPVLRSVCERDPGLGCLAKGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEMLRSKPWFEEPPTALEVEGLAACEGAYSRKYSTLSPLGSGAFGFVWTAVDREENKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_006935746.3
Organism	Felis catus(domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006935746.3

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PASK ( XM_006935745.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006935807.1 Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.

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CloneID	OFb12789
Clone ID Related Accession (Same CDS sequence)	XM_006935745.3 , XM_006935746.3 , XM_006935745.2
Accession Version	XM_006935745.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	PAS domain-containing serine/threonine-protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018730.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006935745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006935807.1
CDS	939..5528
Translation	MEGGGPAESPAVRTTSEPRRSSSSAHRHLSRRNGLAKLCQSRMALSGTRPFWASWVAVSKTQARGRSGLWPLCLFLLRGRALCAGGGAGRSLSCAAVILRHEPVLRSVCERDPGLGCLAKGRWSSYCLSSLAAQNMCASKPHCLAAPERSDAAGSLGGASCCSLLRGLSLGRSTPLLPAPVSNPNKAVFTVDAKTTEILVANDKACQLLGYSSHDLIGQKLTQFFLKPDSDVVKALSEEHVEADGHAAVVFGTVVDIVSRSGEKVPVSVWMKKVKRERSLCCVVMLEPVERVSAWVAFQSDGTITSCDSLFPLLYGYTSGEEVLGQHMADLIPSVQLPPPGELVPQNLKIQRSVGRAKDGTTFPLSLKLKSEPRSEMVEDGRAVPERGYSASVWVFSTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITFLIPGFYDYMELAYDSSLPRPNLTNCLDVGNQSEPGESAVDPHQGWDPAGEAKGRPSLFLPDSRVNGSLVGDHELPRGETLEVAGSREAFAGTGGQADPGGHLPPALPPLPVPRGDNIPEGSLPAQERSSPKDQQNIPEGCPPSQEQSLPEDRRNIPEGCSPSQEQSLSEGQWNISGGCPPSQEQSLPEDQQKFPEGCPPSQEYSLPEDQRNIPEGCPPCQEQSLPEDQRNIPEGSSLAFSKWLFPEGQQQNTPEGSLAALREKSLSKDQQNTLERSPTTQEYSLSKDQRNLPEGCPPAHSQWSLPEAQQDTVLEKEERAAPESLIRDLEGKSGSGPRDTWMFASREDSEGPAPAEGGSRNPGVCGPHQEAQLEGAGVSSAGGSNHRAGSIMPVPHAAGQPAGQRLLPCYPRYGGEQNPQPGSPPRAAPPSFWKPVLDEPWAGESDRDELQTCLIKERLSKWSVVGPPGVSYTEHPLFSAPVSLCDPGGGGPRSRVGSSAAYYALATDLPGVLDAVQAREADGNSFSWNLKELFFGERTDRTFSNRSCTALELVEMASPSLAGSDADLSGPHGPGSQVLDDRELLLLTGTCLHLGEVQPRESCLGRGPAEPSETRVVSSGPYKVSGRENPTCVLPTPGAGSVEVCPLEEPRLNFQVTSTPVKGDSPAMPGAPLQHEIQEGTYSGSCYHRDGSWLSVQFEVKRVELQGSATLFCCWLVKDLLRSHRDSAARTRLLLASLPSSSHSMCELSGPSTGEMLRSKPWFEEPPTALEVEGLAACEGAYSRKYSTLSPLGSGAFGFVWTAVDREENKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVVDVFENQEFFQLVMEKHGSGLDLFAFIDRHPGLDEPLASYIFRQLVSAVAYLRSKSIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGRLFHTFCGTIEYCAPEVLLGNPYKGPELEMWSLGVTLYTLIFEENPFCELEETMEAVIHPPFLVSEDLMNLVSGLLQPIPEQRTTLEKLVTDPWVTQPVNLADYTWDEVCRVNKPESGGLSALSLEMESRCAREVAPEPCGALCPRGGPC

Target ORF information:

RefSeq Version	XM_006935745.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus PAS domain containing serine/threonine kinase (PASK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006935745.2

ORF Insert Sequence:

ATGGAGGGCG GAGGCCCCGC CGAGAGCCCT GCCGTGCGGA CCACCTCCGA GCCCCGCAGG 
TCCTCGTCCT CGGCGCACAG ACACCTGAGC AGAAGGAATG GGCTGGCGAA GCTGTGCCAG 
AGCAGGATGG CCCTCTCCGG TACCCGCCCC TTCTGGGCAT CGTGGGTGGC GGTGTCGAAA 
ACGCAGGCGA GGGGGCGCTC TGGGCTCTGG CCACTCTGTC TCTTCTTGCT GAGAGGACGC 
GCTCTGTGTG CGGGCGGCGG TGCCGGCCGA AGCCTCTCCT GCGCCGCCGT GATTCTTAGG 
CATGAGCCTG TCCTGCGGAG TGTGTGTGAG CGCGACCCTG GGCTGGGCTG CCTTGCAAAA 
GGCAGATGGA GCTCCTACTG TCTGTCCTCG CTGGCTGCCC AGAATATGTG CGCGAGCAAG 
CCTCACTGCC TGGCCGCACC GGAGCGGTCG GACGCGGCCG GGTCCCTGGG GGGCGCGTCC 
TGCTGTTCCC TGCTGCGGGG CTTGTCTTTG GGGCGGTCCA CGCCCCTCCT CCCGGCCCCC 
GTGTCCAACC CGAACAAGGC TGTTTTCACC GTGGACGCCA AGACCACAGA GATCCTGGTC 
GCCAACGACA AGGCTTGCCA GCTCCTAGGG TACAGCAGTC ATGACCTGAT CGGCCAGAAG 
CTCACGCAGT TCTTCCTGAA GCCAGATTCC GACGTGGTGA AGGCCCTCAG CGAAGAGCAT 
GTGGAGGCTG ACGGCCACGC CGCCGTTGTG TTTGGCACGG TGGTGGACAT CGTGAGCCGC 
AGTGGGGAGA AGGTTCCAGT TTCTGTGTGG ATGAAGAAGG TGAAGCGGGA ACGCAGCCTC 
TGCTGTGTGG TCATGCTGGA GCCCGTGGAG CGCGTCTCGG CCTGGGTCGC TTTCCAGAGC 
GATGGAACCA TAACGTCGTG CGACAGCCTC TTCCCTCTCC TGTACGGGTA CACGTCCGGG 
GAGGAGGTGC TCGGGCAGCA CATGGCAGAC CTCATCCCTT CTGTGCAGCT GCCTCCGCCC 
GGTGAGCTCG TCCCACAGAA TCTCAAGATT CAGAGGTCTG TCGGGAGGGC CAAAGATGGC 
ACCACTTTCC CTCTGAGCTT AAAACTGAAA TCCGAGCCCA GAAGCGAGAT GGTGGAAGAC 
GGCAGGGCAG TGCCTGAGCG GGGTTACTCG GCGTCAGTCT GGGTGTTCTC CACCATCAGC 
GGCCTCATCA CCCTCCTGCC CGACGGGACC ATCTATGGCA TCAACCACAG CTTTGCCCTG 
ATGCTGTTCG GTTACGGGAA GACGGAGCTC CTGGGCAAGA ATATCACTTT TCTGATTCCC 
GGTTTCTACG ACTACATGGA GCTCGCATAT GACAGTTCCT TACCACGGCC AAACCTGACC 
AACTGCTTGG ACGTTGGCAA CCAGAGTGAG CCTGGGGAGA GTGCTGTGGA CCCCCACCAG 
GGCTGGGACC CGGCCGGCGA GGCCAAGGGT CGGCCCAGCC TCTTCCTCCC AGATTCCAGG 
GTTAACGGCT CGCTTGTCGG AGACCACGAG CTGCCACGAG GCGAGACCCT AGAGGTAGCG 
GGAAGCCGAG AAGCCTTCGC AGGGACTGGG GGCCAGGCAG ACCCTGGAGG CCACCTCCCT 
CCTGCCCTCC CGCCTCTGCC TGTTCCACGA GGGGACAACA TCCCAGAAGG AAGCCTGCCA 
GCCCAGGAAC GGTCATCGCC CAAGGACCAG CAGAACATCC CAGAAGGATG CCCACCATCC 
CAGGAACAGT CATTGCCTGA GGACCGGCGG AACATCCCAG AAGGATGCTC ACCATCCCAG 
GAACAGTCAT TGTCTGAGGG CCAGTGGAAC ATCTCAGGAG GATGCCCACC ATCCCAGGAA 
CAGTCACTGC CTGAGGACCA GCAGAAGTTC CCAGAAGGAT GCCCACCATC CCAGGAATAC 
TCATTGCCTG AGGACCAGCG GAACATCCCA GAAGGATGCC CACCATGCCA GGAACAGTCC 
TTGCCTGAGG ATCAGCGGAA CATCCCAGAA GGAAGCTCAC TAGCCTTCAG TAAGTGGTTA 
TTTCCTGAGG GCCAGCAGCA GAACACTCCA GAAGGAAGCC TGGCAGCCCT CAGAGAAAAG 
TCATTGTCCA AGGACCAGCA GAACACCCTG GAAAGAAGCC CGACAACCCA GGAATACTCA 
TTGTCCAAGG ACCAGCGGAA CCTCCCAGAA GGATGCCCGC CAGCGCATAG CCAGTGGTCC 
TTACCCGAGG CCCAGCAGGA CACTGTCTTG GAGAAGGAGG AGCGTGCAGC CCCAGAGAGC 
CTCATACGGG ACCTTGAGGG AAAAAGTGGG TCTGGCCCCA GGGACACGTG GATGTTTGCT 
TCACGTGAAG ACTCTGAAGG CCCGGCTCCA GCAGAGGGTG GGAGCAGAAA CCCTGGGGTG 
TGTGGCCCGC ACCAGGAGGC ACAGCTGGAG GGGGCAGGTG TGAGCAGTGC CGGTGGTTCA 
AACCATCGGG CTGGTTCTAT CATGCCCGTG CCCCATGCTG CAGGCCAGCC GGCGGGACAG 
CGCCTCCTGC CGTGCTACCC CAGGTATGGG GGTGAGCAGA ACCCGCAGCC CGGAAGCCCC 
CCCAGGGCAG CGCCACCCTC ATTCTGGAAG CCTGTGCTAG ATGAGCCGTG GGCAGGGGAG 
AGTGACCGAG ATGAGCTGCA GACTTGCCTA ATTAAGGAGC GGCTGTCCAA GTGGAGCGTC 
GTGGGGCCAC CGGGCGTCTC TTACACAGAG CACCCCCTAT TCTCTGCCCC TGTGTCGCTG 
TGTGACCCGG GAGGTGGAGG CCCGCGCAGC CGTGTGGGCA GCTCCGCGGC TTACTACGCA 
CTGGCCACAG ACCTCCCCGG GGTCCTGGAT GCGGTGCAGG CCCGGGAGGC CGATGGGAAT 
TCATTTTCCT GGAATCTCAA GGAACTCTTT TTCGGTGAAC GGACAGACCG AACGTTCTCG 
AACCGTTCCT GTACCGCGTT GGAGCTTGTA GAGATGGCCT CTCCTTCGCT GGCGGGCTCT 
GACGCAGACC TGAGTGGTCC GCACGGGCCC GGGTCACAAG TTCTGGACGA CAGAGAGCTG 
TTGCTCCTGA CGGGCACCTG CTTGCATCTT GGTGAAGTTC AGCCTCGCGA GAGCTGTCTG 
GGGCGCGGCC CGGCCGAGCC CTCTGAGACC CGTGTGGTGT CCTCAGGACC CTACAAAGTG 
AGTGGCAGAG AGAACCCAAC CTGCGTCCTT CCCACGCCGG GAGCTGGCTC CGTGGAGGTG 
TGCCCGTTGG AGGAGCCCAG GCTGAACTTC CAGGTCACCT CCACGCCCGT GAAAGGGGAC 
AGCCCGGCGA TGCCCGGGGC CCCTCTGCAG CATGAGATCC AGGAGGGCAC CTATTCCGGC 
AGCTGTTACC ACCGAGACGG CTCGTGGCTG AGTGTGCAGT TTGAGGTGAA GCGGGTGGAG 
CTCCAGGGCT CCGCGACCCT GTTCTGCTGC TGGCTCGTGA AAGACCTGCT ACGCAGCCAC 
CGGGACTCGG CCGCGCGGAC CCGCCTGCTC CTGGCCAGCC TGCCCAGCTC CAGCCACTCC 
ATGTGCGAGC TTTCTGGACC CAGCACAGGG GAGATGCTCA GGAGCAAGCC CTGGTTTGAG 
GAGCCTCCGA CGGCTCTGGA GGTGGAGGGG CTGGCCGCCT GCGAGGGCGC GTACTCCCGC 
AAGTACAGCA CGCTGAGCCC ACTGGGCAGC GGGGCCTTCG GCTTCGTGTG GACCGCGGTG 
GACAGAGAGG AGAATAAGGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTGTTGGAG 
GATTGTTGGA TTGAGGATCC CAAACTTGGA AAAGTTACTT TGGAGATTGC GATTCTGTCC 
AGGGTGGAGC ACGCCAACAT CATCAAGGTG GTGGACGTCT TTGAAAACCA AGAGTTCTTT 
CAGCTGGTGA TGGAGAAGCA CGGCTCCGGC CTGGACCTCT TTGCGTTCAT CGACCGCCAC 
CCCGGCCTGG ACGAGCCCCT GGCGAGCTAC ATCTTCCGAC AGCTGGTGTC GGCAGTGGCC 
TACCTGCGCT CCAAGAGCAT CATTCACCGG GACATCAAGG ACGAGAACAT CGTGATCGCG 
GAGGACTTTA CCATCAAGCT GATCGACTTC GGCTCGGCTG CCTACCTGGA GAAGGGCAGG 
CTGTTTCACA CGTTCTGTGG GACCATCGAG TACTGTGCGC CCGAGGTGCT CCTGGGAAAC 
CCGTACAAGG GGCCGGAGCT GGAGATGTGG TCACTGGGGG TCACGCTGTA CACGCTGATC 
TTTGAGGAGA ACCCTTTCTG CGAGCTGGAG GAGACCATGG AGGCCGTGAT CCACCCGCCA 
TTCCTGGTGT CAGAAGATCT CATGAACCTC GTGTCTGGGC TGCTGCAGCC TATCCCCGAG 
CAGCGCACCA CCTTGGAGAA GCTGGTGACA GACCCCTGGG TGACACAGCC TGTGAACCTT 
GCTGACTACA CCTGGGATGA GGTGTGTCGA GTGAACAAGC CAGAAAGTGG AGGTCTGTCG 
GCGCTGAGTC TGGAGATGGA GAGCAGGTGC GCGAGGGAAG TGGCCCCAGA GCCATGCGGG 
GCCTTGTGCC CTAGGGGCGG CCCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PASK cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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