Dsim\GD24462 cDNA ORF clone, Drosophila simulans

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsim\GD24462
Entrez Gene ID	6740229
Full Name	GD24462 gene product from transcript GD24462-RB
Synonyms	Dsim_GD24462,GD24462,dsim_GLEANR_8231
Gene Type	protein-coding
Organism	Drosophila simulans

mRNA and Protein(s)

mRNA	Protein	Name
XM_016180775.1	XP_016039995.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Hu TT, Eisen MB, Thornton KR, Andolfatto P
Genome research23(1)89-98(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dsim\GD24462 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsim\GD24462 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODg17300	XM_016180775.1 Latest version!	Drosophila simulans uncharacterized protein (Dsim\GD24462), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsim\GD24462 ( XM_016180775.1 ) cDNA ORF clone, Drosophila simulans -> XP_016039995.1 Drosophila simulans uncharacterized protein (Dsim\GD24462), mRNA.

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CloneID	ODg17300
Clone ID Related Accession (Same CDS sequence)	XM_016180775.1
Accession Version	XM_016180775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_029795). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCCC AACATTCCAG TTGGCGTTTT GGCAAGCGGA GCAAATTACA ATTACGCATC 
AAAGTTTCGC AGGATCCAGA GGACTTCTAC CGCCTGGATC CACAGCGATC GGCCGCCGAA 
AATGCCTTCG AAATATACTC GATGCTCTGC TTGGAGGAGA CGCGGGACAC GAAGCGCCTG 
TTCCTGCTCA ACGCGCTGGC CTCGCTCTGT TTGGGGGCCC GAAAGGGCTC CCACCACAGC 
AACATACGCT TCACCACCGA GGAGCTGCTC TACTGCCTTA GTGCCTCACT GGTGCACACC 
TTTACCCAAG TGCGAGCGGC GGCTCTGCGA ACGATCCGGT ATGCCATTAG CACGCCCAAG 
GACATCAAGG CGTTCAATGC ACTGCAGCTG CAGCACCTGC TCTGCCGCTC CATCGATCTG 
ATGCTGAAGA ACGACGACGA GCGCGTCCAG GCACTGAAGC TGGTCCGCAA GATGCTGGCC 
ATCGCGCCGG AGGACATCAG TCCGGCGGTG GTGCGCTGCC TCGTCTCGCT GGCGGACAGT 
GGCATCGAGG AGAACGACAA TCTGTTGCGT GCCTGCCTGG CCACCCTGGC AGAGTTCGCT 
GTGCTCAATC CGGCGCTGCT GATCGTCTGC GGCGGCGTCA CCTCCATTAC CCGCAATGTG 
CTGGAGTGCC ACAATCCCCG CATCGCCGAG AGCCTGTGCG GCGTGCTTCT GTACCTTCTC 
GAGTGGCCGC AGACGAGGAA CATCTGCGGT GTGCGGCTGG ACTGCCTGGC AGCTCCTTAC 
TGCGACTTCA CCTACCGGCA GAGCATCGTC GATAAGAACA AGGATGCGCG CGAACTTCGC 
TACACCAGCT GTCGGCTGGC ACTGCTGTCC GTGCTGCGCA GCTGGACAGG CACCCTCGAA 
TTCTGTGATC CTAGCAAACC CTCAGGCCTG AAAGCAATAG TCGACGCTCT GTATTTGAAC 
CAGATCGAAG TCAGAAAAGC AATATTGGAT TTGCTGTACG AGCTGCTCAC GCTGCCGCAG 
CCAACCTGGA CGGATGACTA TGCGGTGGCG TTGCAGGCTG TGGATCCGTC GGACTTCCAG 
GACACGTGGC TGCTGAGCAA TGGCTTCGTG TCCGCCGAAG GACGCTCCAT TCTGCCAACG 
CTGGCGGCGC GTGCACCGAG CGTAGTGGAG CAGCACCTGG CGCTGATGCT GTACTGTTTC 
CTCGAGACTG GACTGCTCAA TGCGCTCGTC GAGGTTGTGG TGAGCAGCGA CCAGTTCGTC 
TCGGTGCAGG CTACGGTGCT GATTGGCAAG ATCCTGCAGC TAATGCACAC CCACCTGCCC 
CCGGATATTT GCTGTACGAG TCCGGCACTG CCCACGCTGG TGAGTCATGC CACACTGGGG 
AACCAGCAGG CCAATGCGGC GGTGGCCGCT CTGCAAAACT ACCAGAAGTT GCTGCGCCAG 
CGGCCGGCGT CGTGCAGCTT ATTCCTAGAC AGCATTATCC AGGGCGGTGC TCTCATCCAG 
ACGCGACTGT TTCGTCGGCA CCTCAACGTG CAGGAGCAGG CGGGTCCTGT GCTACAGTTG 
CAGGAAACGG CGGAAGCGGC GGCCTTTGCG GTGCCGCCTG CCACTCAGTT TCGCGGCACC 
CTCGATCGTT CTCGGCTGGA TTCTGTCTCC TCCAGCGACG AGTCCAACTC ACAGGCATCG 
ACCTCGTCGA GGTCCAGCTT TCGACTGAAG CGTAAGTTCC TGCCGCAAGC CTTCTTTGAT 
AACTTCCGAG CCTTCAATCG GCTGCTGACA GACTCCCGTG TGCTGAGCCA AGCGGACGCG 
CATCTTTGGG ACTGGGATGT TATCACCACC ATACTGAGGT CGAATTTAAT CCGCAAACTG 
GACTACACCC AGGGAAAGTT CCTGAAGCGA CTCGTGGACT TCTACAAGCC GCGCAACAAT 
CGGTTCTCTC ACCAGGATTT GGTGCCTGGA CAAAGCCTGC CTACCTATGT CAGTGCCGGA 
CTGGATTTAA TTGACGTCCT CCTGTCCAGC AATGAGCTTG AATGTATGCG CTTTATAACC 
GACTACTTTT CGGACATCAG CCAACAGCTG GCGGCCGTCA CCACATCGAA TCGTGCACAC 
GACTGTCTGT TTAGTCCACA GCATATGAAT AACACGATGT GTCAGCAGTA TTTCCTCTAC 
ATCGGACGCA TGTGCCGAAC CGTAAAAGGC ATCGAAGTAC TCAAAAACAC CACCGTATTT 
GAATACTTGA TCAATTTGGT GCGGGTGACG GACCACGTCT GCTATGTGAA GCTAATTGTG 
TCCGGCCTGA ACTACAGCTA CGAGAAGCTA CCACGTCAGG TGCTGGAAAA GGCGCTCACG 
TCGGCGAAGA CACGCGGACG TCTGTACACC ACCCAGTTCA TGGCAGTGCT GCTGCGTGCC 
CGTCTGCCTC ACTTTGAGGT ATGGGGCATT CCATTGATCA TTAACCAGAC GCGGGATACG 
GACCGCAGCG TGGGGCTGGC GGCAATGGAT GTACTGGAGG AGGCCTGCCA CGACAAGTAC 
TACCTGGAGG AGATTGTCAG TCGGTGGCCG AATCTAACGC ATCGTGGTGA TGCAGGACGA 
CTGCTGATGG CCCGCTATTA CTCACTGCCC CGCGGCCTGA ACAGTACGAT GGCGCGGATC 
GAGGAGGAGA TCCGCTACTG GCGGAATGGG TACAACAAGC GCTACGTGCT CCTGGTCGAA 
GCCGACACCC ACTCAAGCCT GACGCTGCAC ATCCGCAACG AGGATGGCTA CTACTCGCGT 
CGCAATTGCA ACCAGAGACC ACAGACTGTA CCACCAAACG TTGCACCCCA TCTGTACGGG 
CAAATGGCAC AGACTGGCCA GGGTATGACA GCTCTGCGGA AGCACGGAGA TCTGCCGCAG 
CTATTGGAAC TGCTCACACG CGCCAAATGC ACGGACGATG CCGAGTGCCT GGAGCTGAAG 
GCGGCTATTT GGGCACTGGC CCACGCCTCA ACCCACTCCA ATGGAATTGA ATACTTTGTC 
GAACTGAATG CCAGACTGTA CGAGAAGCTA ATTGTTCTGG TGACCAAATG TGAGGTATAT 
TCGGTGCGGG CCACCTGTTT TAGTGCCTTA GGACTTATCG CAGGCACACA GGCAGGTGCC 
AATATCCTTT TTAAGTTGAA CTGGCTGAGC GTGCGACATG ACAAGAACAC AATGTGGCCC 
GTGCATCAGC CGGAAGATTG GATGTCCAGC CAGTACACAC CGGTACGACA TTACTACGAG 
GACGTGCCGG CGGATAATGT GACCATGATG GATGACTATA TAGAAAGATT CTACGAAACG 
GGCTCAGACT ATTGGCACAT GCTCGCCGAT CAGACTGATG CGAGTGGTGG TGGTGGGGGT 
GTAGCCGGCA CAGTCGTCGG TATGCATCCC ATAGCGCAGG ATCCAAATAC AACGATTACG 
ACCTCGGACA GTGTTCGCAC CACGACCGAT GATGTGCACC CCAGAATGTT GGTTGGAGTG 
GCTGCCAAAT CAAAGACTTT GCCGGAGGGC AGCAATCTGC GCCAGGGCAA GCACCAGCGC 
TCACTCTCCG AATCGAAGAC CACGGATGTG ATCAGCTTGT TGGGCAGTGG TGTAGCGACG 
ATGTCGGGAG CTGCTCTATA CCCAACGCCG TATCAGCATC GAATACGCTA CAACTCGTGC 
ACGGATTCAA ACACTTCCGG CGTTAGCAGC TGCGAATCGG TGACCGGACG CACTGCAGCT 
GCATATGCCG CCGCAGCCAA TGAGTTGCAG CAATTCCCCT TGAGCCCTAT TCCAAGCATG 
TCCAATCTGC TCGAGAGCGA TGAGCTGATC CGTCGACATC ACCTAGCCAC CAGTATGCTG 
CCCTCCACCC TCAGTCCTAT GGCGATGAAG GGCTATGCCC AGCTACGCTT CCTGCGCAAG 
CACAGCCGAC CCGTATTCTC CGAATCATCG GCGGAGTTCT ACGACTTTGC GGAAATACTG 
GACACACCAG AGGTTCAGAT GCGCAAGCTC GACTGGACCT ATTCCCAACG CCGGCTCAAA 
GTGCGCAGCT TGGACCGCCA GCTAAGCGAT GTTTATAGAC GCCTCTCCGC GGACGAGTTG 
AATGTGCTGC TGCCTACGCT TAACGCTCCC AAGTTCCTGC CTCCTAATGA TTCGAAAGGG 
CCGTGCTATG CGGGCATCTG CTTGCCTAAG AACGTTCTGG ACCTGTTTCC GACGCGCAAC 
CTCAGTCGAA CGTACGTGTC GCGGGATATC CAGGACCAAG ATATAGTGGG CATAAACCTG 
CTGAGCAGCA TGGTCCGGCC ACAGTGTCTC AATGACTCAC TCACCAATGA GGGCGGCGAC 
GAGTCATCGG TTATATCATC GCTGTCGGAC GTCTCGTCTG CGAGTCGGCG ACAACCGCGA 
CAACTGCAGC AAGGCGCCAA GCATTCGCGA AGTCTGTGTC TCCATTGTGC CCGCTCCCCG 
CGACAGCAGC GTAATGACAG CGGCAGCCAT AGTAATGGTG GAGGAGGCGC ATCCCTCGCT 
CCCTGCGAGC TGTACAGCAG TGCAGCAGCA GCTCTGGTCG CAGCCGGCAT CCAGGCTGGA 
CCAGTTTTGG CTAAAAAGAG CGGCTCATCT GCTTCAGCGG CACTTGGCCC CGACATCAGC 
TTCCATTCGC CAGAGAGCAT GCTGAGCGAG GATAGCCTGC CGGACAGGCT TACGGCCTCC 
ATCCTGTACA ACGTGCAGCG TCTGGCCAAT CCCGTTAGTG CCAAGCAGTC GAAAATGGCT 
CTGTTGGAGC TTAAACAGAA GCACCCGCAC GCCTTTCAGG ATATTTGCCT TTATTCGGAG 
TCCTGCAAAA CGATGGGCCG GAGTAGCTAT CGGATGATAG CCCGCCGCTT CCTGCAGGAG 
CTCTTTCTGG ATCTTAATTT CGACTCGTTC TACGTGGAGC CTCAGTTCAT AATCGGGGCA 
CGCAAGTTTT CCGCTGAGGA AAAAGCAGAG GCCACCGCCC CCACGGCAAT GCAGGCCATG 
CCGTTCAAGC AAACGATTCT CAAGCCCAAG CCGGCGGCCC AACTGGCTAG CGTGTATGAG 
ACTAGTTGGG AGAACCTGCT GATGGACCAG TCGCCACGGA TAAACAAGTC ATCACCTGGC 
GATCAACCGG CCAATGCTCA AACGAAAGCA AAGCCCCGGA ACCAGCTGCA CATTGACGAC 
ACGGAGAGTT CGTTGTCTAG TACCGACGGC GAAAATGATT CCGGTGAGGA TGTTTGCGAC 
GGCAGTGGAG CCGGCACCCA GGCCAGCTCG GTGACCACCG CTCATACAGC CCTGCCCACA 
GGAAACAGCA ACCGTAGCAG CATTGGCACA TGCACAGCAA GCCAGCTGGT GCCAGGCGCG 
TCAACTTCAA ACTCCGCTGA TGCTGGAACA AGCGCATCTG ATAATGCCAG TACAAGTCTA 
AAGAACGAAA ACCGGCAATT CACACGCGGA CGTTTCTATA CGCTGGAGCT CGATTTGAGC 
TGCACCAAGA ACAAGTTTCC CATCAAGAAT CGAAGCCAGC GAATACCAAC GCCGCCACCA 
ATCGAAAGTT TAATTCAGTC CAAGCGCACG GCTCTGAGAG ATGTGAATAC ATGTGAGCCG 
GAAATCATGC AGCGGACCGG TGGCAGCAAT GAGTCCTCGT CAGCCCTTAG ATATTCCACT 
GTGACCAAGA GCTTGTCGGC ATCGATGGCC AGGCCGGTGG GCAGTCTGTA CTGCGAGAAG 
CGCTTGCAGA GCTCCAAGTC GGAAGCAGTT CTCGATGAGG TGGCGAAGGC GAGAAGATCA 
TCCGGTGGTG TGGGACTGAC AACCGTGCGC CAACTCCAAG ACGCACTGGC CGAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016039995.1
CDS	211..6030
Translation	MASQHSSWRFGKRSKLQLRIKVSQDPEDFYRLDPQRSAAENAFEIYSMLCLEETRDTKRLFLLNALASLCLGARKGSHHSNIRFTTEELLYCLSASLVHTFTQVRAAALRTIRYAISTPKDIKAFNALQLQHLLCRSIDLMLKNDDERVQALKLVRKMLAIAPEDISPAVVRCLVSLADSGIEENDNLLRACLATLAEFAVLNPALLIVCGGVTSITRNVLECHNPRIAESLCGVLLYLLEWPQTRNICGVRLDCLAAPYCDFTYRQSIVDKNKDARELRYTSCRLALLSVLRSWTGTLEFCDPSKPSGLKAIVDALYLNQIEVRKAILDLLYELLTLPQPTWTDDYAVALQAVDPSDFQDTWLLSNGFVSAEGRSILPTLAARAPSVVEQHLALMLYCFLETGLLNALVEVVVSSDQFVSVQATVLIGKILQLMHTHLPPDICCTSPALPTLVSHATLGNQQANAAVAALQNYQKLLRQRPASCSLFLDSIIQGGALIQTRLFRRHLNVQEQAGPVLQLQETAEAAAFAVPPATQFRGTLDRSRLDSVSSSDESNSQASTSSRSSFRLKRKFLPQAFFDNFRAFNRLLTDSRVLSQADAHLWDWDVITTILRSNLIRKLDYTQGKFLKRLVDFYKPRNNRFSHQDLVPGQSLPTYVSAGLDLIDVLLSSNELECMRFITDYFSDISQQLAAVTTSNRAHDCLFSPQHMNNTMCQQYFLYIGRMCRTVKGIEVLKNTTVFEYLINLVRVTDHVCYVKLIVSGLNYSYEKLPRQVLEKALTSAKTRGRLYTTQFMAVLLRARLPHFEVWGIPLIINQTRDTDRSVGLAAMDVLEEACHDKYYLEEIVSRWPNLTHRGDAGRLLMARYYSLPRGLNSTMARIEEEIRYWRNGYNKRYVLLVEADTHSSLTLHIRNEDGYYSRRNCNQRPQTVPPNVAPHLYGQMAQTGQGMTALRKHGDLPQLLELLTRAKCTDDAECLELKAAIWALAHASTHSNGIEYFVELNARLYEKLIVLVTKCEVYSVRATCFSALGLIAGTQAGANILFKLNWLSVRHDKNTMWPVHQPEDWMSSQYTPVRHYYEDVPADNVTMMDDYIERFYETGSDYWHMLADQTDASGGGGGVAGTVVGMHPIAQDPNTTITTSDSVRTTTDDVHPRMLVGVAAKSKTLPEGSNLRQGKHQRSLSESKTTDVISLLGSGVATMSGAALYPTPYQHRIRYNSCTDSNTSGVSSCESVTGRTAAAYAAAANELQQFPLSPIPSMSNLLESDELIRRHHLATSMLPSTLSPMAMKGYAQLRFLRKHSRPVFSESSAEFYDFAEILDTPEVQMRKLDWTYSQRRLKVRSLDRQLSDVYRRLSADELNVLLPTLNAPKFLPPNDSKGPCYAGICLPKNVLDLFPTRNLSRTYVSRDIQDQDIVGINLLSSMVRPQCLNDSLTNEGGDESSVISSLSDVSSASRRQPRQLQQGAKHSRSLCLHCARSPRQQRNDSGSHSNGGGGASLAPCELYSSAAAALVAAGIQAGPVLAKKSGSSASAALGPDISFHSPESMLSEDSLPDRLTASILYNVQRLANPVSAKQSKMALLELKQKHPHAFQDICLYSESCKTMGRSSYRMIARRFLQELFLDLNFDSFYVEPQFIIGARKFSAEEKAEATAPTAMQAMPFKQTILKPKPAAQLASVYETSWENLLMDQSPRINKSSPGDQPANAQTKAKPRNQLHIDDTESSLSSTDGENDSGEDVCDGSGAGTQASSVTTAHTALPTGNSNRSSIGTCTASQLVPGASTSNSADAGTSASDNASTSLKNENRQFTRGRFYTLELDLSCTKNKFPIKNRSQRIPTPPPIESLIQSKRTALRDVNTCEPEIMQRTGGSNESSSALRYSTVTKSLSASMARPVGSLYCEKRLQSSKSEAVLDEVAKARRSSGGVGLTTVRQLQDALAED

Target ORF information:

RefSeq Version	XM_016180775.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein (Dsim\GD24462), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016180775.1

ORF Insert Sequence:

ATGGCCTCCC AACATTCCAG TTGGCGTTTT GGCAAGCGGA GCAAATTACA ATTACGCATC 
AAAGTTTCGC AGGATCCAGA GGACTTCTAC CGCCTGGATC CACAGCGATC GGCCGCCGAA 
AATGCCTTCG AAATATACTC GATGCTCTGC TTGGAGGAGA CGCGGGACAC GAAGCGCCTG 
TTCCTGCTCA ACGCGCTGGC CTCGCTCTGT TTGGGGGCCC GAAAGGGCTC CCACCACAGC 
AACATACGCT TCACCACCGA GGAGCTGCTC TACTGCCTTA GTGCCTCACT GGTGCACACC 
TTTACCCAAG TGCGAGCGGC GGCTCTGCGA ACGATCCGGT ATGCCATTAG CACGCCCAAG 
GACATCAAGG CGTTCAATGC ACTGCAGCTG CAGCACCTGC TCTGCCGCTC CATCGATCTG 
ATGCTGAAGA ACGACGACGA GCGCGTCCAG GCACTGAAGC TGGTCCGCAA GATGCTGGCC 
ATCGCGCCGG AGGACATCAG TCCGGCGGTG GTGCGCTGCC TCGTCTCGCT GGCGGACAGT 
GGCATCGAGG AGAACGACAA TCTGTTGCGT GCCTGCCTGG CCACCCTGGC AGAGTTCGCT 
GTGCTCAATC CGGCGCTGCT GATCGTCTGC GGCGGCGTCA CCTCCATTAC CCGCAATGTG 
CTGGAGTGCC ACAATCCCCG CATCGCCGAG AGCCTGTGCG GCGTGCTTCT GTACCTTCTC 
GAGTGGCCGC AGACGAGGAA CATCTGCGGT GTGCGGCTGG ACTGCCTGGC AGCTCCTTAC 
TGCGACTTCA CCTACCGGCA GAGCATCGTC GATAAGAACA AGGATGCGCG CGAACTTCGC 
TACACCAGCT GTCGGCTGGC ACTGCTGTCC GTGCTGCGCA GCTGGACAGG CACCCTCGAA 
TTCTGTGATC CTAGCAAACC CTCAGGCCTG AAAGCAATAG TCGACGCTCT GTATTTGAAC 
CAGATCGAAG TCAGAAAAGC AATATTGGAT TTGCTGTACG AGCTGCTCAC GCTGCCGCAG 
CCAACCTGGA CGGATGACTA TGCGGTGGCG TTGCAGGCTG TGGATCCGTC GGACTTCCAG 
GACACGTGGC TGCTGAGCAA TGGCTTCGTG TCCGCCGAAG GACGCTCCAT TCTGCCAACG 
CTGGCGGCGC GTGCACCGAG CGTAGTGGAG CAGCACCTGG CGCTGATGCT GTACTGTTTC 
CTCGAGACTG GACTGCTCAA TGCGCTCGTC GAGGTTGTGG TGAGCAGCGA CCAGTTCGTC 
TCGGTGCAGG CTACGGTGCT GATTGGCAAG ATCCTGCAGC TAATGCACAC CCACCTGCCC 
CCGGATATTT GCTGTACGAG TCCGGCACTG CCCACGCTGG TGAGTCATGC CACACTGGGG 
AACCAGCAGG CCAATGCGGC GGTGGCCGCT CTGCAAAACT ACCAGAAGTT GCTGCGCCAG 
CGGCCGGCGT CGTGCAGCTT ATTCCTAGAC AGCATTATCC AGGGCGGTGC TCTCATCCAG 
ACGCGACTGT TTCGTCGGCA CCTCAACGTG CAGGAGCAGG CGGGTCCTGT GCTACAGTTG 
CAGGAAACGG CGGAAGCGGC GGCCTTTGCG GTGCCGCCTG CCACTCAGTT TCGCGGCACC 
CTCGATCGTT CTCGGCTGGA TTCTGTCTCC TCCAGCGACG AGTCCAACTC ACAGGCATCG 
ACCTCGTCGA GGTCCAGCTT TCGACTGAAG CGTAAGTTCC TGCCGCAAGC CTTCTTTGAT 
AACTTCCGAG CCTTCAATCG GCTGCTGACA GACTCCCGTG TGCTGAGCCA AGCGGACGCG 
CATCTTTGGG ACTGGGATGT TATCACCACC ATACTGAGGT CGAATTTAAT CCGCAAACTG 
GACTACACCC AGGGAAAGTT CCTGAAGCGA CTCGTGGACT TCTACAAGCC GCGCAACAAT 
CGGTTCTCTC ACCAGGATTT GGTGCCTGGA CAAAGCCTGC CTACCTATGT CAGTGCCGGA 
CTGGATTTAA TTGACGTCCT CCTGTCCAGC AATGAGCTTG AATGTATGCG CTTTATAACC 
GACTACTTTT CGGACATCAG CCAACAGCTG GCGGCCGTCA CCACATCGAA TCGTGCACAC 
GACTGTCTGT TTAGTCCACA GCATATGAAT AACACGATGT GTCAGCAGTA TTTCCTCTAC 
ATCGGACGCA TGTGCCGAAC CGTAAAAGGC ATCGAAGTAC TCAAAAACAC CACCGTATTT 
GAATACTTGA TCAATTTGGT GCGGGTGACG GACCACGTCT GCTATGTGAA GCTAATTGTG 
TCCGGCCTGA ACTACAGCTA CGAGAAGCTA CCACGTCAGG TGCTGGAAAA GGCGCTCACG 
TCGGCGAAGA CACGCGGACG TCTGTACACC ACCCAGTTCA TGGCAGTGCT GCTGCGTGCC 
CGTCTGCCTC ACTTTGAGGT ATGGGGCATT CCATTGATCA TTAACCAGAC GCGGGATACG 
GACCGCAGCG TGGGGCTGGC GGCAATGGAT GTACTGGAGG AGGCCTGCCA CGACAAGTAC 
TACCTGGAGG AGATTGTCAG TCGGTGGCCG AATCTAACGC ATCGTGGTGA TGCAGGACGA 
CTGCTGATGG CCCGCTATTA CTCACTGCCC CGCGGCCTGA ACAGTACGAT GGCGCGGATC 
GAGGAGGAGA TCCGCTACTG GCGGAATGGG TACAACAAGC GCTACGTGCT CCTGGTCGAA 
GCCGACACCC ACTCAAGCCT GACGCTGCAC ATCCGCAACG AGGATGGCTA CTACTCGCGT 
CGCAATTGCA ACCAGAGACC ACAGACTGTA CCACCAAACG TTGCACCCCA TCTGTACGGG 
CAAATGGCAC AGACTGGCCA GGGTATGACA GCTCTGCGGA AGCACGGAGA TCTGCCGCAG 
CTATTGGAAC TGCTCACACG CGCCAAATGC ACGGACGATG CCGAGTGCCT GGAGCTGAAG 
GCGGCTATTT GGGCACTGGC CCACGCCTCA ACCCACTCCA ATGGAATTGA ATACTTTGTC 
GAACTGAATG CCAGACTGTA CGAGAAGCTA ATTGTTCTGG TGACCAAATG TGAGGTATAT 
TCGGTGCGGG CCACCTGTTT TAGTGCCTTA GGACTTATCG CAGGCACACA GGCAGGTGCC 
AATATCCTTT TTAAGTTGAA CTGGCTGAGC GTGCGACATG ACAAGAACAC AATGTGGCCC 
GTGCATCAGC CGGAAGATTG GATGTCCAGC CAGTACACAC CGGTACGACA TTACTACGAG 
GACGTGCCGG CGGATAATGT GACCATGATG GATGACTATA TAGAAAGATT CTACGAAACG 
GGCTCAGACT ATTGGCACAT GCTCGCCGAT CAGACTGATG CGAGTGGTGG TGGTGGGGGT 
GTAGCCGGCA CAGTCGTCGG TATGCATCCC ATAGCGCAGG ATCCAAATAC AACGATTACG 
ACCTCGGACA GTGTTCGCAC CACGACCGAT GATGTGCACC CCAGAATGTT GGTTGGAGTG 
GCTGCCAAAT CAAAGACTTT GCCGGAGGGC AGCAATCTGC GCCAGGGCAA GCACCAGCGC 
TCACTCTCCG AATCGAAGAC CACGGATGTG ATCAGCTTGT TGGGCAGTGG TGTAGCGACG 
ATGTCGGGAG CTGCTCTATA CCCAACGCCG TATCAGCATC GAATACGCTA CAACTCGTGC 
ACGGATTCAA ACACTTCCGG CGTTAGCAGC TGCGAATCGG TGACCGGACG CACTGCAGCT 
GCATATGCCG CCGCAGCCAA TGAGTTGCAG CAATTCCCCT TGAGCCCTAT TCCAAGCATG 
TCCAATCTGC TCGAGAGCGA TGAGCTGATC CGTCGACATC ACCTAGCCAC CAGTATGCTG 
CCCTCCACCC TCAGTCCTAT GGCGATGAAG GGCTATGCCC AGCTACGCTT CCTGCGCAAG 
CACAGCCGAC CCGTATTCTC CGAATCATCG GCGGAGTTCT ACGACTTTGC GGAAATACTG 
GACACACCAG AGGTTCAGAT GCGCAAGCTC GACTGGACCT ATTCCCAACG CCGGCTCAAA 
GTGCGCAGCT TGGACCGCCA GCTAAGCGAT GTTTATAGAC GCCTCTCCGC GGACGAGTTG 
AATGTGCTGC TGCCTACGCT TAACGCTCCC AAGTTCCTGC CTCCTAATGA TTCGAAAGGG 
CCGTGCTATG CGGGCATCTG CTTGCCTAAG AACGTTCTGG ACCTGTTTCC GACGCGCAAC 
CTCAGTCGAA CGTACGTGTC GCGGGATATC CAGGACCAAG ATATAGTGGG CATAAACCTG 
CTGAGCAGCA TGGTCCGGCC ACAGTGTCTC AATGACTCAC TCACCAATGA GGGCGGCGAC 
GAGTCATCGG TTATATCATC GCTGTCGGAC GTCTCGTCTG CGAGTCGGCG ACAACCGCGA 
CAACTGCAGC AAGGCGCCAA GCATTCGCGA AGTCTGTGTC TCCATTGTGC CCGCTCCCCG 
CGACAGCAGC GTAATGACAG CGGCAGCCAT AGTAATGGTG GAGGAGGCGC ATCCCTCGCT 
CCCTGCGAGC TGTACAGCAG TGCAGCAGCA GCTCTGGTCG CAGCCGGCAT CCAGGCTGGA 
CCAGTTTTGG CTAAAAAGAG CGGCTCATCT GCTTCAGCGG CACTTGGCCC CGACATCAGC 
TTCCATTCGC CAGAGAGCAT GCTGAGCGAG GATAGCCTGC CGGACAGGCT TACGGCCTCC 
ATCCTGTACA ACGTGCAGCG TCTGGCCAAT CCCGTTAGTG CCAAGCAGTC GAAAATGGCT 
CTGTTGGAGC TTAAACAGAA GCACCCGCAC GCCTTTCAGG ATATTTGCCT TTATTCGGAG 
TCCTGCAAAA CGATGGGCCG GAGTAGCTAT CGGATGATAG CCCGCCGCTT CCTGCAGGAG 
CTCTTTCTGG ATCTTAATTT CGACTCGTTC TACGTGGAGC CTCAGTTCAT AATCGGGGCA 
CGCAAGTTTT CCGCTGAGGA AAAAGCAGAG GCCACCGCCC CCACGGCAAT GCAGGCCATG 
CCGTTCAAGC AAACGATTCT CAAGCCCAAG CCGGCGGCCC AACTGGCTAG CGTGTATGAG 
ACTAGTTGGG AGAACCTGCT GATGGACCAG TCGCCACGGA TAAACAAGTC ATCACCTGGC 
GATCAACCGG CCAATGCTCA AACGAAAGCA AAGCCCCGGA ACCAGCTGCA CATTGACGAC 
ACGGAGAGTT CGTTGTCTAG TACCGACGGC GAAAATGATT CCGGTGAGGA TGTTTGCGAC 
GGCAGTGGAG CCGGCACCCA GGCCAGCTCG GTGACCACCG CTCATACAGC CCTGCCCACA 
GGAAACAGCA ACCGTAGCAG CATTGGCACA TGCACAGCAA GCCAGCTGGT GCCAGGCGCG 
TCAACTTCAA ACTCCGCTGA TGCTGGAACA AGCGCATCTG ATAATGCCAG TACAAGTCTA 
AAGAACGAAA ACCGGCAATT CACACGCGGA CGTTTCTATA CGCTGGAGCT CGATTTGAGC 
TGCACCAAGA ACAAGTTTCC CATCAAGAAT CGAAGCCAGC GAATACCAAC GCCGCCACCA 
ATCGAAAGTT TAATTCAGTC CAAGCGCACG GCTCTGAGAG ATGTGAATAC ATGTGAGCCG 
GAAATCATGC AGCGGACCGG TGGCAGCAAT GAGTCCTCGT CAGCCCTTAG ATATTCCACT 
GTGACCAAGA GCTTGTCGGC ATCGATGGCC AGGCCGGTGG GCAGTCTGTA CTGCGAGAAG 
CGCTTGCAGA GCTCCAAGTC GGAAGCAGTT CTCGATGAGG TGGCGAAGGC GAGAAGATCA 
TCCGGTGGTG TGGGACTGAC AACCGTGCGC CAACTCCAAG ACGCACTGGC CGAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Genome research23(1)89-98(2013 Jan)
Hu TT,Eisen MB,Thornton KR,Andolfatto P

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Dsim\GD24462 cDNA ORF clone, Drosophila simulans

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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