Dpse\GA23474 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA23474
Entrez Gene ID	6900648
Full Name	GA23474 gene product from transcript GA23474-RG
Synonyms	GA23474,dpse_GLEANR_12322
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015187611.1	XP_015043097.1	uncharacterized protein, isoform K
XM_015187610.1	XP_015043096.1	uncharacterized protein, isoform I
XM_015187609.1	XP_015043095.1	uncharacterized protein, isoform G
XM_002134985.2	XP_002135021.2	uncharacterized protein, isoform H
XM_015187606.1	XP_015043092.1	uncharacterized protein, isoform B
XM_015187607.1	XP_015043093.1	uncharacterized protein, isoform E
XM_015187608.1	XP_015043094.1	uncharacterized protein, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA23474 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA23474 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe26409	XM_015187608.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODe25829	XM_002134985.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant H (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODe26407	XM_015187606.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODe26411	XM_015187610.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant I (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODe26408	XM_015187607.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe26410	XM_015187609.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe26412	XM_015187611.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant K (Dpse\GA23474), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA23474 ( XM_015187608.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043094.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA23474), mRNA.

Price & Availability↑

CloneID	ODe26409
Clone ID Related Accession (Same CDS sequence)	XM_015187608.1
Accession Version	XM_015187608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043094.1
CDS	220..1005
Translation	MYNGIGLTTARGSGTNGHVQRNCAFVRPGKKDKDYRSEDDIKKLDAQLNRPPNKEILDHDRKRKIEVKCLEFEEILEKQGRTPEDIKSQVDSFRQKLVGQGKTDAPKDEFGRVAARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKER

Target ORF information:

RefSeq Version	XM_015187608.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187608.1

ORF Insert Sequence:

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_002134985.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002135021.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant H (Dpse\GA23474), mRNA.

Price & Availability↑

CloneID	ODe25829
Clone ID Related Accession (Same CDS sequence)	XM_002134985.2
Accession Version	XM_002134985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform H
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_002134985.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGAAAAA GCGCGACAAG 
AAGCTGAAAA AGAAGTCACG GGCCCGTGCC AGCTCCTCGG ACTCGGAGCC CTCCAACTCA 
CCGTCGCGTA ATGAACGCAA AGGCAAGAAG TCGGGCAAAT CCCGCGATCC AACAGCTACC 
AAAGCTGAAG ACAATGGCAC ATCGCGGGAG AATCCCTTCC GGAGCAGGTC CCGCGAACGT 
CGCAGGGAAT TAAAGCGGCC CAACGAATCA TCGGCCGACG GTCGAAGGGA CAAGCGCGAG 
CGTAGCGATA GTCGCAGTCG ACGTCCCAAG TCCAATGAAC GGGGGGCAAC CACCCGTACT 
CCACCTCGCA AAGAGCCGGA AAGAAAGCGC GAACGTTCTA AGGAGAGATT TCGATCAAAA 
GAAAGGCAAC GATCAAAAGA AAGACAGCGA TCAAAGGAAA GACTTCGTTC GAAGGAAAGA 
CAACGCTCGA AAGAAAGACA GCGATCAAAG GAACGACCAA AATCGAAGGA GAGACAGAGG 
TCGAGGGAAA GACAGCGATC GAAGGAGAGG ATCCGCTCCA AAGAAAGACA GCGTTCAAAG 
GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG TCTAAGGATA GACAGAGATC TAAGGATAGG 
CAGAGATCTA AGGATAGGCA GAGGTCAAAA GAGAGACAGA GGTCAAAGGA GAGGCAGCGA 
TCAAAGGCGA GACTTTATTC GAAAGAGAGA CTGCGATCCA AGGAGAGGGC GCGATCAAAG 
GATAAACCAG TAGTTGAAAT CAAGCGCGAC AGATCAAAGG ATCGCCAGCG ATCAAAGGAG 
AGGAAACGAT CGGTGGATAA GGCACCCTCG AAGGAGAGAG ATATGTCCAA AGGTCGACAA 
AGATCAAAGG AGAGACAGCG CTCCAAAGAA AGACGACGTT CTAAGGATCG CGGTCAACAA 
TCGAATGAGA AAAAGCGTTC CAAGGAGCGA CAACGATCAA AAGACCGTCA ACGATCCAAG 
GAGCGACAGG GAGTGCCAAA CCTAGCCGTC TCTAAGGAAC GTACCCGGCG CCAGAGATCG 
CCATCGACCT CGGCCAAAAA TTCAACCGAT AATCGCAAGC CTACTCGCAG TTCTAGATCA 
CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG GCCGCAGTTG TCGGGAGCAG CCGACGCAGT 
CATTCCCCAG AGGTTCCACC CAAGAAGGCT GCGCCACCGG CATTCAATCC CTTTAAGGCT 
GCAGAAGACA CTGTCAACGA TATCCTGGGC ACCAAGTCGG TGATTGTCGA ACTGGAGAAG 
ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC 
TCGTCTGCAT CCCGGACACC TTCGCCCAAG CCCAAGCCTG GCAAACTGCG ACGACGCAGC 
AAAACATCCG ATCCTATCAA GAAGGAGAGG AGCCCCAAGC GAGATCGTCG TACCCCGCGC 
CAGACCAGCG TGAAGCGTGA ACGTTCAAGC ACTCCACAAA AGCCCAAGCC AAAGGGAAAG 
GCGACCACAC CCAGTCCGAA GCCAGCAAAG CGACGCTCGC ACAGCTCAAG CTCTTCGGAA 
CTGCGCTACT CGCCAGCAGA ACGACATCCA GAGCGCTATC AGGACATCAT ACAAGACAAG 
AAGCGGCCAT CGAAGGCACG CGACCTTTCC ACAACGAAGC CCGCGCCCGT GGTGCGCCTG 
AGGGCCCAGA GCGATGACGG CAGCGATGCC GATGCGAGTG GCTCCAATGT GGATGCAGCA 
GCACTTGACG AGTTCCAGCA GAGCAAGCAA GAGCGCGAGG AGCTGCAGGA GCTGCGTATG 
CTGGAGCAGC TCAAGTCGGG GATCGCCGCC AAGGCAAAGC AGAAGATCAA GACTATGGAG 
AAGAACGGAG ACGTCAGCAA GGACTCTGCC AAGGAGGGCA GTGAACCCTT AGTTGCTGCA 
CTCGCCGACA GCAGTCTGGT GGACGCCATC GTCTCCAAGG TGTCCACGGC CACTGTGGCT 
GCAGCGGAGA GTGCAGCTCG TCGAAGTCGG AGTCGTTCAC GCCGCAGCAG TCACAGCCGC 
AGCAAGCGGC ACACGAGTGG CAGTCATCAT CATCATCACT CCAGTAGTCG CTCAAGATCA 
CGCTCCAGCT CCAGCAGCAG TGCCAGCGGC TCGCGCAGTT CTAGGTCCCG CTCTGGCTCG 
CAATCATCTC GTTCCAGCTG CTCATCCCAA AGCAGCTCGG GCAGCTCCTC CAGCAGCGGC 
TCAGGCACAT CACAGTCTGG CTCACGTTCC CCATCTATAC CACGGCGTCG GGGCTCACCC 
AGCTTTCTAG ACAGACGCCG CATTACGAGT GCTCGCAAGC GACCCATTCC CTACCACCAC 
AAGGCAGCCG GTGGCACTGA GGGGGGTGCT GCAGAGGCAG AGGAGGCCTC CAGCTGCTGC 
TCGAGCTGCT TCAGTCGGGC CAGCACACCG GCCACGCCCA TCTGCACTCC GCTGCGGAAC 
AGTCGGAGCC CCTCGATGGC CGCCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002135021.2
CDS	220..3669
Translation	MYNGIGLTTARGSGTNGHVQRNCAFVRPGKKDKDYRSEDDIKKLDAQLNRPPNKEILDHDRKRKIEVKCLEFEEILEKQGRTPEDIKSQVDSFRQKLVGQGKTDAPKDEFGRVAARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_002134985.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant H (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002134985.2

ORF Insert Sequence:

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGAAAAA GCGCGACAAG 
AAGCTGAAAA AGAAGTCACG GGCCCGTGCC AGCTCCTCGG ACTCGGAGCC CTCCAACTCA 
CCGTCGCGTA ATGAACGCAA AGGCAAGAAG TCGGGCAAAT CCCGCGATCC AACAGCTACC 
AAAGCTGAAG ACAATGGCAC ATCGCGGGAG AATCCCTTCC GGAGCAGGTC CCGCGAACGT 
CGCAGGGAAT TAAAGCGGCC CAACGAATCA TCGGCCGACG GTCGAAGGGA CAAGCGCGAG 
CGTAGCGATA GTCGCAGTCG ACGTCCCAAG TCCAATGAAC GGGGGGCAAC CACCCGTACT 
CCACCTCGCA AAGAGCCGGA AAGAAAGCGC GAACGTTCTA AGGAGAGATT TCGATCAAAA 
GAAAGGCAAC GATCAAAAGA AAGACAGCGA TCAAAGGAAA GACTTCGTTC GAAGGAAAGA 
CAACGCTCGA AAGAAAGACA GCGATCAAAG GAACGACCAA AATCGAAGGA GAGACAGAGG 
TCGAGGGAAA GACAGCGATC GAAGGAGAGG ATCCGCTCCA AAGAAAGACA GCGTTCAAAG 
GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG TCTAAGGATA GACAGAGATC TAAGGATAGG 
CAGAGATCTA AGGATAGGCA GAGGTCAAAA GAGAGACAGA GGTCAAAGGA GAGGCAGCGA 
TCAAAGGCGA GACTTTATTC GAAAGAGAGA CTGCGATCCA AGGAGAGGGC GCGATCAAAG 
GATAAACCAG TAGTTGAAAT CAAGCGCGAC AGATCAAAGG ATCGCCAGCG ATCAAAGGAG 
AGGAAACGAT CGGTGGATAA GGCACCCTCG AAGGAGAGAG ATATGTCCAA AGGTCGACAA 
AGATCAAAGG AGAGACAGCG CTCCAAAGAA AGACGACGTT CTAAGGATCG CGGTCAACAA 
TCGAATGAGA AAAAGCGTTC CAAGGAGCGA CAACGATCAA AAGACCGTCA ACGATCCAAG 
GAGCGACAGG GAGTGCCAAA CCTAGCCGTC TCTAAGGAAC GTACCCGGCG CCAGAGATCG 
CCATCGACCT CGGCCAAAAA TTCAACCGAT AATCGCAAGC CTACTCGCAG TTCTAGATCA 
CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG GCCGCAGTTG TCGGGAGCAG CCGACGCAGT 
CATTCCCCAG AGGTTCCACC CAAGAAGGCT GCGCCACCGG CATTCAATCC CTTTAAGGCT 
GCAGAAGACA CTGTCAACGA TATCCTGGGC ACCAAGTCGG TGATTGTCGA ACTGGAGAAG 
ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC 
TCGTCTGCAT CCCGGACACC TTCGCCCAAG CCCAAGCCTG GCAAACTGCG ACGACGCAGC 
AAAACATCCG ATCCTATCAA GAAGGAGAGG AGCCCCAAGC GAGATCGTCG TACCCCGCGC 
CAGACCAGCG TGAAGCGTGA ACGTTCAAGC ACTCCACAAA AGCCCAAGCC AAAGGGAAAG 
GCGACCACAC CCAGTCCGAA GCCAGCAAAG CGACGCTCGC ACAGCTCAAG CTCTTCGGAA 
CTGCGCTACT CGCCAGCAGA ACGACATCCA GAGCGCTATC AGGACATCAT ACAAGACAAG 
AAGCGGCCAT CGAAGGCACG CGACCTTTCC ACAACGAAGC CCGCGCCCGT GGTGCGCCTG 
AGGGCCCAGA GCGATGACGG CAGCGATGCC GATGCGAGTG GCTCCAATGT GGATGCAGCA 
GCACTTGACG AGTTCCAGCA GAGCAAGCAA GAGCGCGAGG AGCTGCAGGA GCTGCGTATG 
CTGGAGCAGC TCAAGTCGGG GATCGCCGCC AAGGCAAAGC AGAAGATCAA GACTATGGAG 
AAGAACGGAG ACGTCAGCAA GGACTCTGCC AAGGAGGGCA GTGAACCCTT AGTTGCTGCA 
CTCGCCGACA GCAGTCTGGT GGACGCCATC GTCTCCAAGG TGTCCACGGC CACTGTGGCT 
GCAGCGGAGA GTGCAGCTCG TCGAAGTCGG AGTCGTTCAC GCCGCAGCAG TCACAGCCGC 
AGCAAGCGGC ACACGAGTGG CAGTCATCAT CATCATCACT CCAGTAGTCG CTCAAGATCA 
CGCTCCAGCT CCAGCAGCAG TGCCAGCGGC TCGCGCAGTT CTAGGTCCCG CTCTGGCTCG 
CAATCATCTC GTTCCAGCTG CTCATCCCAA AGCAGCTCGG GCAGCTCCTC CAGCAGCGGC 
TCAGGCACAT CACAGTCTGG CTCACGTTCC CCATCTATAC CACGGCGTCG GGGCTCACCC 
AGCTTTCTAG ACAGACGCCG CATTACGAGT GCTCGCAAGC GACCCATTCC CTACCACCAC 
AAGGCAGCCG GTGGCACTGA GGGGGGTGCT GCAGAGGCAG AGGAGGCCTC CAGCTGCTGC 
TCGAGCTGCT TCAGTCGGGC CAGCACACCG GCCACGCCCA TCTGCACTCC GCTGCGGAAC 
AGTCGGAGCC CCTCGATGGC CGCCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_015187606.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043092.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23474), mRNA.

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CloneID	ODe26407
Clone ID Related Accession (Same CDS sequence)	XM_015187606.1
Accession Version	XM_015187606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGAAAAA GCGCGACAAG 
AAGCTGAAAA AGAAGTCACG GGCCCGTGCC AGCTCCTCGG ACTCGGAGCC CTCCAACTCA 
CCGTCGCGTA ATGAACGCAA AGGCAAGAAG TCGGGCAAAT CCCGCGATCC AACAGCTACC 
AAAGCTGAAG ACAATGGCAC ATCGCGGGAG AATCCCTTCC GGAGCAGGTC CCGCGAACGT 
CGCAGGGAAT TAAAGCGGCC CAACGAATCA TCGGCCGACG GTCGAAGGGA CAAGCGCGAG 
CGTAGCGATA GTCGCAGTCG ACGTCCCAAG TCCAATGAAC GGGGGGCAAC CACCCGTACT 
CCACCTCGCA AAGAGCCGGA AAGAAAGCGC GAACGTTCTA AGGAGAGATT TCGATCAAAA 
GAAAGGCAAC GATCAAAAGA AAGACAGCGA TCAAAGGAAA GACTTCGTTC GAAGGAAAGA 
CAACGCTCGA AAGAAAGACA GCGATCAAAG GAACGACCAA AATCGAAGGA GAGACAGAGG 
TCGAGGGAAA GACAGCGATC GAAGGAGAGG ATCCGCTCCA AAGAAAGACA GCGTTCAAAG 
GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG TCTAAGGATA GACAGAGATC TAAGGATAGG 
CAGAGATCTA AGGATAGGCA GAGGTCAAAA GAGAGACAGA GGTCAAAGGA GAGGCAGCGA 
TCAAAGGCGA GACTTTATTC GAAAGAGAGA CTGCGATCCA AGGAGAGGGC GCGATCAAAG 
GATAAACCAG TAGTTGAAAT CAAGCGCGAC AGATCAAAGG ATCGCCAGCG ATCAAAGGAG 
AGGAAACGAT CGGTGGATAA GGCACCCTCG AAGGAGAGAG ATATGTCCAA AGGTCGACAA 
AGATCAAAGG AGAGACAGCG CTCCAAAGAA AGACGACGTT CTAAGGATCG CGGTCAACAA 
TCGAATGAGA AAAAGCGTTC CAAGGAGCGA CAACGATCAA AAGACCGTCA ACGATCCAAG 
GAGCGACAGG GAGTGCCAAA CCTAGCCGTC TCTAAGGAAC GTACCCGGCG CCAGAGATCG 
CCATCGACCT CGGCCAAAAA TTCAACCGAT AATCGCAAGC CTACTCGCAG TTCTAGATCA 
CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG GCCGCAGTTG TCGGGAGCAG CCGACGCAGT 
CATTCCCCAG AGGTTCCACC CAAGAAGGCT GCGCCACCGG CATTCAATCC CTTTAAGGCT 
GCAGAAGACA CTGTCAACGA TATCCTGGGC ACCAAGTCGG TGATTGTCGA ACTGGAGAAG 
ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC 
TCGTCTGCAT CCCGGACACC TTCGCCCAAG CCCAAGCCTG GCAAACTGCG ACGACGCAGC 
AAAACATCCG ATCCTATCAA GAAGGAGAGG AGCCCCAAGC GAGATCGTCG TACCCCGCGC 
CAGACCAGCG TGAAGCGTGA ACGTTCAAGC ACTCCACAAA AGCCCAAGCC AAAGGGAAAG 
GCGACCACAC CCAGTCCGAA GCCAGCAAAG CGACGCTCGC ACAGCTCAAG CTCTTCGGAA 
CTGCGCTACT CGCCAGCAGA ACGACATCCA GAGCGCTATC AGGACATCAT ACAAGACAAG 
AAGCGGCCAT CGAAGGCACG CGACCTTTCC ACAACGAAGC CCGCGCCCGT GGTGCGCCTG 
AGGGCCCAGA GCGATGACGG CAGCGATGCC GATGCGAGTG GCTCCAATGT GGATGCAGCA 
GCACTTGACG AGTTCCAGCA GAGCAAGCAA GAGCGCGAGG AGCTGCAGGA GCTGCGTATG 
CTGGAGCAGC TCAAGTCGGG GATCGCCGCC AAGGCAAAGC AGAAGATCAA GACTATGGAG 
AAGAACGGAG ACGTCAGCAA GGACTCTGCC AAGGAGGGCA GTGAAGTAAG TGGCAACGAA 
CTGGTGACGG CTACTAAACG TAATTCGCTA AGTGAATTCC TTGTTGCTAA CAATGTAACC 
GCTTTGATTA ACCCATTGAC AACGACAACG ACTACGACAA CACCCCCGCC ACTCATGGCT 
GTGATCCTAC CGCTGGCACA GCCACATCCG CAGCTACAGG AGCAGAAGCC GCAGCAGCAG 
CATCAGTCTG TGGAGGAGCC AAACCAAAAA AGAGACGACA CCACGACGCC GCCCATTTGC 
AAAGCGCCCC AAGTGGCTGC CAATGGCGAC GTTAAAAGTC CACCAGCAAC GAACATGATA 
ACTAAGATCC ATCAAAGGCC TCCACAGCAG CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC 
CAGCACCAGC AGCAGTCGCA TCAACACCAG CATCCACACC AGCAACAGCA TTCGCACCCA 
CACCAGACGA AGCGAATCTT TCACAACCGA ACGCTGAACA ATAACCCTAA CAGTAGACAT 
AATACTAACA ACAACAATAG CAACAACAAC ATTCATGCAT GTGGTGGTGG TGTCCCTCAT 
TCCAATAGCA ATCCCGGTAT GCTGCCGTTC CTGGCCGGCA CGCCGGGCAC CTACAACCGC 
ACAACGAACC GCCTTAACCA CGGTCCGCTG CTGACGGCCA CCCATTACAA TATATGCAAG 
AACCATCAGA ACAGCATGCA ACAGCAAGCT CTGCTCGGTC GACGCGACCA CCAGCAGCAG 
CATCTGGCGC ATGGTCTTGT TTATAACCCT AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT 
TTGCTGGCCG CTGGCGGCCA GCATCATGGA CGTCATGCCG TTGGGGGCCT TTTGGGCCTG 
GGCGGTGGTG GCGGACCTGC TGCGGGCCTG GCCTTGCTTA GTCATCCCCA GCATGCGAGG 
ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA GCGGCTGCGG CCGTGGCAGC CAATAGTATA 
AGCTATCATC ATCATCACCA GCAACAGAAA CCGAAAATTG TAATAAAACC ATTCAAACTT 
CTGGATCCAC AGCCCTTAGT TGCTGCACTC GCCGACAGCA GTCTGGTGGA CGCCATCGTC 
TCCAAGGTGT CCACGGCCAC TGTGGCTGCA GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT 
CGTTCACGCC GCAGCAGTCA CAGCCGCAGC AAGCGGCACA CGAGTGGCAG TCATCATCAT 
CATCACTCCA GTAGTCGCTC AAGATCACGC TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG 
CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA TCATCTCGTT CCAGCTGCTC ATCCCAAAGC 
AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA GGCACATCAC AGTCTGGCTC ACGTTCCCCA 
TCTATACCAC GGCGTCGGGG CTCACCCAGC TTTCTAGACA GACGCCGCAT TACGAGTGCT 
CGCAAGCGAC CCATTCCCTA CCACCACAAG GCAGCCGGTG GCACTGAGGG GGGTGCTGCA 
GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG AGCTGCTTCA GTCGGGCCAG CACACCGGCC 
ACGCCCATCT GCACTCCGCT GCGGAACAGT CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043092.1
CDS	222..4718
Translation	MYNGIGLTTARGSGTNGHVQRNCAFVRPGKKDKDYRSEDDIKKLDAQLNRPPNKEILDHDRKRKIEVKCLEFEEILEKQGRTPEDIKSQVDSFRQKLVGQGKTDAPKDEFGRVAARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEVSGNELVTATKRNSLSEFLVANNVTALINPLTTTTTTTTPPPLMAVILPLAQPHPQLQEQKPQQQHQSVEEPNQKRDDTTTPPICKAPQVAANGDVKSPPATNMITKIHQRPPQQQQQHQQQQQHQHQQQSHQHQHPHQQQHSHPHQTKRIFHNRTLNNNPNSRHNTNNNNSNNNIHACGGGVPHSNSNPGMLPFLAGTPGTYNRTTNRLNHGPLLTATHYNICKNHQNSMQQQALLGRRDHQQQHLAHGLVYNPSLFGLGQQQALLAAGGQHHGRHAVGGLLGLGGGGGPAAGLALLSHPQHARTGSISFNAAAAAAAVAANSISYHHHHQQQKPKIVIKPFKLLDPQPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_015187606.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187606.1

ORF Insert Sequence:

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGAAAAA GCGCGACAAG 
AAGCTGAAAA AGAAGTCACG GGCCCGTGCC AGCTCCTCGG ACTCGGAGCC CTCCAACTCA 
CCGTCGCGTA ATGAACGCAA AGGCAAGAAG TCGGGCAAAT CCCGCGATCC AACAGCTACC 
AAAGCTGAAG ACAATGGCAC ATCGCGGGAG AATCCCTTCC GGAGCAGGTC CCGCGAACGT 
CGCAGGGAAT TAAAGCGGCC CAACGAATCA TCGGCCGACG GTCGAAGGGA CAAGCGCGAG 
CGTAGCGATA GTCGCAGTCG ACGTCCCAAG TCCAATGAAC GGGGGGCAAC CACCCGTACT 
CCACCTCGCA AAGAGCCGGA AAGAAAGCGC GAACGTTCTA AGGAGAGATT TCGATCAAAA 
GAAAGGCAAC GATCAAAAGA AAGACAGCGA TCAAAGGAAA GACTTCGTTC GAAGGAAAGA 
CAACGCTCGA AAGAAAGACA GCGATCAAAG GAACGACCAA AATCGAAGGA GAGACAGAGG 
TCGAGGGAAA GACAGCGATC GAAGGAGAGG ATCCGCTCCA AAGAAAGACA GCGTTCAAAG 
GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG TCTAAGGATA GACAGAGATC TAAGGATAGG 
CAGAGATCTA AGGATAGGCA GAGGTCAAAA GAGAGACAGA GGTCAAAGGA GAGGCAGCGA 
TCAAAGGCGA GACTTTATTC GAAAGAGAGA CTGCGATCCA AGGAGAGGGC GCGATCAAAG 
GATAAACCAG TAGTTGAAAT CAAGCGCGAC AGATCAAAGG ATCGCCAGCG ATCAAAGGAG 
AGGAAACGAT CGGTGGATAA GGCACCCTCG AAGGAGAGAG ATATGTCCAA AGGTCGACAA 
AGATCAAAGG AGAGACAGCG CTCCAAAGAA AGACGACGTT CTAAGGATCG CGGTCAACAA 
TCGAATGAGA AAAAGCGTTC CAAGGAGCGA CAACGATCAA AAGACCGTCA ACGATCCAAG 
GAGCGACAGG GAGTGCCAAA CCTAGCCGTC TCTAAGGAAC GTACCCGGCG CCAGAGATCG 
CCATCGACCT CGGCCAAAAA TTCAACCGAT AATCGCAAGC CTACTCGCAG TTCTAGATCA 
CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG GCCGCAGTTG TCGGGAGCAG CCGACGCAGT 
CATTCCCCAG AGGTTCCACC CAAGAAGGCT GCGCCACCGG CATTCAATCC CTTTAAGGCT 
GCAGAAGACA CTGTCAACGA TATCCTGGGC ACCAAGTCGG TGATTGTCGA ACTGGAGAAG 
ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC 
TCGTCTGCAT CCCGGACACC TTCGCCCAAG CCCAAGCCTG GCAAACTGCG ACGACGCAGC 
AAAACATCCG ATCCTATCAA GAAGGAGAGG AGCCCCAAGC GAGATCGTCG TACCCCGCGC 
CAGACCAGCG TGAAGCGTGA ACGTTCAAGC ACTCCACAAA AGCCCAAGCC AAAGGGAAAG 
GCGACCACAC CCAGTCCGAA GCCAGCAAAG CGACGCTCGC ACAGCTCAAG CTCTTCGGAA 
CTGCGCTACT CGCCAGCAGA ACGACATCCA GAGCGCTATC AGGACATCAT ACAAGACAAG 
AAGCGGCCAT CGAAGGCACG CGACCTTTCC ACAACGAAGC CCGCGCCCGT GGTGCGCCTG 
AGGGCCCAGA GCGATGACGG CAGCGATGCC GATGCGAGTG GCTCCAATGT GGATGCAGCA 
GCACTTGACG AGTTCCAGCA GAGCAAGCAA GAGCGCGAGG AGCTGCAGGA GCTGCGTATG 
CTGGAGCAGC TCAAGTCGGG GATCGCCGCC AAGGCAAAGC AGAAGATCAA GACTATGGAG 
AAGAACGGAG ACGTCAGCAA GGACTCTGCC AAGGAGGGCA GTGAAGTAAG TGGCAACGAA 
CTGGTGACGG CTACTAAACG TAATTCGCTA AGTGAATTCC TTGTTGCTAA CAATGTAACC 
GCTTTGATTA ACCCATTGAC AACGACAACG ACTACGACAA CACCCCCGCC ACTCATGGCT 
GTGATCCTAC CGCTGGCACA GCCACATCCG CAGCTACAGG AGCAGAAGCC GCAGCAGCAG 
CATCAGTCTG TGGAGGAGCC AAACCAAAAA AGAGACGACA CCACGACGCC GCCCATTTGC 
AAAGCGCCCC AAGTGGCTGC CAATGGCGAC GTTAAAAGTC CACCAGCAAC GAACATGATA 
ACTAAGATCC ATCAAAGGCC TCCACAGCAG CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC 
CAGCACCAGC AGCAGTCGCA TCAACACCAG CATCCACACC AGCAACAGCA TTCGCACCCA 
CACCAGACGA AGCGAATCTT TCACAACCGA ACGCTGAACA ATAACCCTAA CAGTAGACAT 
AATACTAACA ACAACAATAG CAACAACAAC ATTCATGCAT GTGGTGGTGG TGTCCCTCAT 
TCCAATAGCA ATCCCGGTAT GCTGCCGTTC CTGGCCGGCA CGCCGGGCAC CTACAACCGC 
ACAACGAACC GCCTTAACCA CGGTCCGCTG CTGACGGCCA CCCATTACAA TATATGCAAG 
AACCATCAGA ACAGCATGCA ACAGCAAGCT CTGCTCGGTC GACGCGACCA CCAGCAGCAG 
CATCTGGCGC ATGGTCTTGT TTATAACCCT AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT 
TTGCTGGCCG CTGGCGGCCA GCATCATGGA CGTCATGCCG TTGGGGGCCT TTTGGGCCTG 
GGCGGTGGTG GCGGACCTGC TGCGGGCCTG GCCTTGCTTA GTCATCCCCA GCATGCGAGG 
ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA GCGGCTGCGG CCGTGGCAGC CAATAGTATA 
AGCTATCATC ATCATCACCA GCAACAGAAA CCGAAAATTG TAATAAAACC ATTCAAACTT 
CTGGATCCAC AGCCCTTAGT TGCTGCACTC GCCGACAGCA GTCTGGTGGA CGCCATCGTC 
TCCAAGGTGT CCACGGCCAC TGTGGCTGCA GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT 
CGTTCACGCC GCAGCAGTCA CAGCCGCAGC AAGCGGCACA CGAGTGGCAG TCATCATCAT 
CATCACTCCA GTAGTCGCTC AAGATCACGC TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG 
CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA TCATCTCGTT CCAGCTGCTC ATCCCAAAGC 
AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA GGCACATCAC AGTCTGGCTC ACGTTCCCCA 
TCTATACCAC GGCGTCGGGG CTCACCCAGC TTTCTAGACA GACGCCGCAT TACGAGTGCT 
CGCAAGCGAC CCATTCCCTA CCACCACAAG GCAGCCGGTG GCACTGAGGG GGGTGCTGCA 
GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG AGCTGCTTCA GTCGGGCCAG CACACCGGCC 
ACGCCCATCT GCACTCCGCT GCGGAACAGT CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_015187610.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043096.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant I (Dpse\GA23474), mRNA.

Price & Availability↑

CloneID	ODe26411
Clone ID Related Accession (Same CDS sequence)	XM_015187610.1
Accession Version	XM_015187610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTCCT CGGAAATCTA CACCAATACG ACCACCTCCA CGGCTATGTC GGCGGTGGGG 
ATAACTTCAG CTGCTTCTGC TTCTGCTGCT GCCTCAGTGG CAACCACAAC AACAACAACA 
GCCACCACAT TGCAAGTGGT GAAAGCGAGA AAACGCCGCA AGCGTCGGAA ACGCGCAGGT 
CACGCAATTG GTGCCGCCTC CACCACCACC ACCATCACAG CTGCAGCCGC CACAGCCAGC 
CACCTCGGCA GTCCCCACAC CCACACACAC CCCCAAGCAG GAAGCGTTTC CCTACCGACA 
GCAAACGGTG GCAGGAAGTG CAAGGCCCAA TACTTCCAGC ACGACAATCG GGAGTACTTG 
GCAAAGTACG AGAGCTTTCA ACTCACGGCG CACCACACGG CATCGGGCGC AGCTGCGCTG 
AACTATGCGG CGGTGGCGAA AAAGTTCAAG CCCTTGCGGC CGGCGTTAAC AAGCGGCGCC 
AAGAAAACCA GGCGACCAGG AATGCCCAGA AAGTCGAACC CTGACTGCGC CTGCGCCTGC 
CAATCCGCAG TGACGGAGCC GGCCGCGGAA CCCAGCCAGC GAACAGCTGT TGCCACGCAC 
CAGGACTTGC CACTGTCCAC GTCGCAGTGG GGAAACGGAA GCGCAAACGC GAACGCATCA 
AAGATCAATA TCGATGGCCA GATACTGCTC GAGCTGCTGA AGCACTCCTC GAACACATTG 
CTGGAGACAC TGCTCGGTGC TGGCAGTGCT GTGTCATCGA GTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAACCCTT AGTTGCTGCA CTCGCCGACA GCAGTCTGGT GGACGCCATC 
GTCTCCAAGG TGTCCACGGC CACTGTGGCT GCAGCGGAGA GTGCAGCTCG TCGAAGTCGG 
AGTCGTTCAC GCCGCAGCAG TCACAGCCGC AGCAAGCGGC ACACGAGTGG CAGTCATCAT 
CATCATCACT CCAGTAGTCG CTCAAGATCA CGCTCCAGCT CCAGCAGCAG TGCCAGCGGC 
TCGCGCAGTT CTAGGTCCCG CTCTGGCTCG CAATCATCTC GTTCCAGCTG CTCATCCCAA 
AGCAGCTCGG GCAGCTCCTC CAGCAGCGGC TCAGGCACAT CACAGTCTGG CTCACGTTCC 
CCATCTATAC CACGGCGTCG GGGCTCACCC AGCTTTCTAG ACAGACGCCG CATTACGAGT 
GCTCGCAAGC GACCCATTCC CTACCACCAC AAGGCAGCCG GTGGCACTGA GGGGGGTGCT 
GCAGAGGCAG AGGAGGCCTC CAGCTGCTGC TCGAGCTGCT TCAGTCGGGC CAGCACACCG 
GCCACGCCCA TCTGCACTCC GCTGCGGAAC AGTCGGAGCC CCTCGATGGC CGCCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043096.1
CDS	26..3925
Translation	MLSSEIYTNTTTSTAMSAVGITSAASASAAASVATTTTTTATTLQVVKARKRRKRRKRAGHAIGAASTTTTITAAAATASHLGSPHTHTHPQAGSVSLPTANGGRKCKAQYFQHDNREYLAKYESFQLTAHHTASGAAALNYAAVAKKFKPLRPALTSGAKKTRRPGMPRKSNPDCACACQSAVTEPAAEPSQRTAVATHQDLPLSTSQWGNGSANANASKINIDGQILLELLKHSSNTLLETLLGAGSAVSSSARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKDSFQWLSCSFKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_015187610.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant I (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187610.1

ORF Insert Sequence:

ATGCTCTCCT CGGAAATCTA CACCAATACG ACCACCTCCA CGGCTATGTC GGCGGTGGGG 
ATAACTTCAG CTGCTTCTGC TTCTGCTGCT GCCTCAGTGG CAACCACAAC AACAACAACA 
GCCACCACAT TGCAAGTGGT GAAAGCGAGA AAACGCCGCA AGCGTCGGAA ACGCGCAGGT 
CACGCAATTG GTGCCGCCTC CACCACCACC ACCATCACAG CTGCAGCCGC CACAGCCAGC 
CACCTCGGCA GTCCCCACAC CCACACACAC CCCCAAGCAG GAAGCGTTTC CCTACCGACA 
GCAAACGGTG GCAGGAAGTG CAAGGCCCAA TACTTCCAGC ACGACAATCG GGAGTACTTG 
GCAAAGTACG AGAGCTTTCA ACTCACGGCG CACCACACGG CATCGGGCGC AGCTGCGCTG 
AACTATGCGG CGGTGGCGAA AAAGTTCAAG CCCTTGCGGC CGGCGTTAAC AAGCGGCGCC 
AAGAAAACCA GGCGACCAGG AATGCCCAGA AAGTCGAACC CTGACTGCGC CTGCGCCTGC 
CAATCCGCAG TGACGGAGCC GGCCGCGGAA CCCAGCCAGC GAACAGCTGT TGCCACGCAC 
CAGGACTTGC CACTGTCCAC GTCGCAGTGG GGAAACGGAA GCGCAAACGC GAACGCATCA 
AAGATCAATA TCGATGGCCA GATACTGCTC GAGCTGCTGA AGCACTCCTC GAACACATTG 
CTGGAGACAC TGCTCGGTGC TGGCAGTGCT GTGTCATCGA GTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAACCCTT AGTTGCTGCA CTCGCCGACA GCAGTCTGGT GGACGCCATC 
GTCTCCAAGG TGTCCACGGC CACTGTGGCT GCAGCGGAGA GTGCAGCTCG TCGAAGTCGG 
AGTCGTTCAC GCCGCAGCAG TCACAGCCGC AGCAAGCGGC ACACGAGTGG CAGTCATCAT 
CATCATCACT CCAGTAGTCG CTCAAGATCA CGCTCCAGCT CCAGCAGCAG TGCCAGCGGC 
TCGCGCAGTT CTAGGTCCCG CTCTGGCTCG CAATCATCTC GTTCCAGCTG CTCATCCCAA 
AGCAGCTCGG GCAGCTCCTC CAGCAGCGGC TCAGGCACAT CACAGTCTGG CTCACGTTCC 
CCATCTATAC CACGGCGTCG GGGCTCACCC AGCTTTCTAG ACAGACGCCG CATTACGAGT 
GCTCGCAAGC GACCCATTCC CTACCACCAC AAGGCAGCCG GTGGCACTGA GGGGGGTGCT 
GCAGAGGCAG AGGAGGCCTC CAGCTGCTGC TCGAGCTGCT TCAGTCGGGC CAGCACACCG 
GCCACGCCCA TCTGCACTCC GCTGCGGAAC AGTCGGAGCC CCTCGATGGC CGCCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_015187607.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043093.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA23474), mRNA.

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CloneID	ODe26408
Clone ID Related Accession (Same CDS sequence)	XM_015187607.1
Accession Version	XM_015187607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAAGTAAG TGGCAACGAA CTGGTGACGG CTACTAAACG TAATTCGCTA 
AGTGAATTCC TTGTTGCTAA CAATGTAACC GCTTTGATTA ACCCATTGAC AACGACAACG 
ACTACGACAA CACCCCCGCC ACTCATGGCT GTGATCCTAC CGCTGGCACA GCCACATCCG 
CAGCTACAGG AGCAGAAGCC GCAGCAGCAG CATCAGTCTG TGGAGGAGCC AAACCAAAAA 
AGAGACGACA CCACGACGCC GCCCATTTGC AAAGCGCCCC AAGTGGCTGC CAATGGCGAC 
GTTAAAAGTC CACCAGCAAC GAACATGATA ACTAAGATCC ATCAAAGGCC TCCACAGCAG 
CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC CAGCACCAGC AGCAGTCGCA TCAACACCAG 
CATCCACACC AGCAACAGCA TTCGCACCCA CACCAGACGA AGCGAATCTT TCACAACCGA 
ACGCTGAACA ATAACCCTAA CAGTAGACAT AATACTAACA ACAACAATAG CAACAACAAC 
ATTCATGCAT GTGGTGGTGG TGTCCCTCAT TCCAATAGCA ATCCCGGTAT GCTGCCGTTC 
CTGGCCGGCA CGCCGGGCAC CTACAACCGC ACAACGAACC GCCTTAACCA CGGTCCGCTG 
CTGACGGCCA CCCATTACAA TATATGCAAG AACCATCAGA ACAGCATGCA ACAGCAAGCT 
CTGCTCGGTC GACGCGACCA CCAGCAGCAG CATCTGGCGC ATGGTCTTGT TTATAACCCT 
AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT TTGCTGGCCG CTGGCGGCCA GCATCATGGA 
CGTCATGCCG TTGGGGGCCT TTTGGGCCTG GGCGGTGGTG GCGGACCTGC TGCGGGCCTG 
GCCTTGCTTA GTCATCCCCA GCATGCGAGG ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA 
GCGGCTGCGG CCGTGGCAGC CAATAGTATA AGCTATCATC ATCATCACCA GCAACAGAAA 
CCGAAAATTG TAATAAAACC ATTCAAACTT CTGGATCCAC AGCCCTTAGT TGCTGCACTC 
GCCGACAGCA GTCTGGTGGA CGCCATCGTC TCCAAGGTGT CCACGGCCAC TGTGGCTGCA 
GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT CGTTCACGCC GCAGCAGTCA CAGCCGCAGC 
AAGCGGCACA CGAGTGGCAG TCATCATCAT CATCACTCCA GTAGTCGCTC AAGATCACGC 
TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA 
TCATCTCGTT CCAGCTGCTC ATCCCAAAGC AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA 
GGCACATCAC AGTCTGGCTC ACGTTCCCCA TCTATACCAC GGCGTCGGGG CTCACCCAGC 
TTTCTAGACA GACGCCGCAT TACGAGTGCT CGCAAGCGAC CCATTCCCTA CCACCACAAG 
GCAGCCGGTG GCACTGAGGG GGGTGCTGCA GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG 
AGCTGCTTCA GTCGGGCCAG CACACCGGCC ACGCCCATCT GCACTCCGCT GCGGAACAGT 
CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043093.1
CDS	223..4749
Translation	MYNGIGLTTARGSGTNGHVQRNCAFVRPGKKDKDYRSEDDIKKLDAQLNRPPNKEILDHDRKRKIEVKCLEFEEILEKQGRTPEDIKSQVDSFRQKLVGQGKTDAPKDEFGRVAARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKDSFQWLSCSFKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEVSGNELVTATKRNSLSEFLVANNVTALINPLTTTTTTTTPPPLMAVILPLAQPHPQLQEQKPQQQHQSVEEPNQKRDDTTTPPICKAPQVAANGDVKSPPATNMITKIHQRPPQQQQQHQQQQQHQHQQQSHQHQHPHQQQHSHPHQTKRIFHNRTLNNNPNSRHNTNNNNSNNNIHACGGGVPHSNSNPGMLPFLAGTPGTYNRTTNRLNHGPLLTATHYNICKNHQNSMQQQALLGRRDHQQQHLAHGLVYNPSLFGLGQQQALLAAGGQHHGRHAVGGLLGLGGGGGPAAGLALLSHPQHARTGSISFNAAAAAAAVAANSISYHHHHQQQKPKIVIKPFKLLDPQPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_015187607.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187607.1

ORF Insert Sequence:

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAAGTAAG TGGCAACGAA CTGGTGACGG CTACTAAACG TAATTCGCTA 
AGTGAATTCC TTGTTGCTAA CAATGTAACC GCTTTGATTA ACCCATTGAC AACGACAACG 
ACTACGACAA CACCCCCGCC ACTCATGGCT GTGATCCTAC CGCTGGCACA GCCACATCCG 
CAGCTACAGG AGCAGAAGCC GCAGCAGCAG CATCAGTCTG TGGAGGAGCC AAACCAAAAA 
AGAGACGACA CCACGACGCC GCCCATTTGC AAAGCGCCCC AAGTGGCTGC CAATGGCGAC 
GTTAAAAGTC CACCAGCAAC GAACATGATA ACTAAGATCC ATCAAAGGCC TCCACAGCAG 
CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC CAGCACCAGC AGCAGTCGCA TCAACACCAG 
CATCCACACC AGCAACAGCA TTCGCACCCA CACCAGACGA AGCGAATCTT TCACAACCGA 
ACGCTGAACA ATAACCCTAA CAGTAGACAT AATACTAACA ACAACAATAG CAACAACAAC 
ATTCATGCAT GTGGTGGTGG TGTCCCTCAT TCCAATAGCA ATCCCGGTAT GCTGCCGTTC 
CTGGCCGGCA CGCCGGGCAC CTACAACCGC ACAACGAACC GCCTTAACCA CGGTCCGCTG 
CTGACGGCCA CCCATTACAA TATATGCAAG AACCATCAGA ACAGCATGCA ACAGCAAGCT 
CTGCTCGGTC GACGCGACCA CCAGCAGCAG CATCTGGCGC ATGGTCTTGT TTATAACCCT 
AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT TTGCTGGCCG CTGGCGGCCA GCATCATGGA 
CGTCATGCCG TTGGGGGCCT TTTGGGCCTG GGCGGTGGTG GCGGACCTGC TGCGGGCCTG 
GCCTTGCTTA GTCATCCCCA GCATGCGAGG ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA 
GCGGCTGCGG CCGTGGCAGC CAATAGTATA AGCTATCATC ATCATCACCA GCAACAGAAA 
CCGAAAATTG TAATAAAACC ATTCAAACTT CTGGATCCAC AGCCCTTAGT TGCTGCACTC 
GCCGACAGCA GTCTGGTGGA CGCCATCGTC TCCAAGGTGT CCACGGCCAC TGTGGCTGCA 
GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT CGTTCACGCC GCAGCAGTCA CAGCCGCAGC 
AAGCGGCACA CGAGTGGCAG TCATCATCAT CATCACTCCA GTAGTCGCTC AAGATCACGC 
TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA 
TCATCTCGTT CCAGCTGCTC ATCCCAAAGC AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA 
GGCACATCAC AGTCTGGCTC ACGTTCCCCA TCTATACCAC GGCGTCGGGG CTCACCCAGC 
TTTCTAGACA GACGCCGCAT TACGAGTGCT CGCAAGCGAC CCATTCCCTA CCACCACAAG 
GCAGCCGGTG GCACTGAGGG GGGTGCTGCA GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG 
AGCTGCTTCA GTCGGGCCAG CACACCGGCC ACGCCCATCT GCACTCCGCT GCGGAACAGT 
CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_015187609.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043095.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA23474), mRNA.

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CloneID	ODe26410
Clone ID Related Accession (Same CDS sequence)	XM_015187609.1
Accession Version	XM_015187609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CCAATACGAC CACCTCCACG 
GCTATGTCGG CGGTGGGGAT AACTTCAGCT GCTTCTGCTT CTGCTGCTGC CTCAGTGGCA 
ACCACAACAA CAACAACAGC CACCACATTG CAAGTGGTGA AAGCGAGAAA ACGCCGCAAG 
CGTCGGAAAC GCGCAGGTCA CGCAATTGGT GCCGCCTCCA CCACCACCAC CATCACAGCT 
GCAGCCGCCA CAGCCAGCCA CCTCGGCAGT CCCCACACCC ACACACACCC CCAAGCAGGA 
AGCGTTTCCC TACCGACAGC AAACGGTGGC AGGAAGTGCA AGGCCCAATA CTTCCAGCAC 
GACAATCGGG AGTACTTGGC AAAGTACGAG AGCTTTCAAC TCACGGCGCA CCACACGGCA 
TCGGGCGCAG CTGCGCTGAA CTATGCGGCG GTGGCGAAAA AGTTCAAGCC CTTGCGGCCG 
GCGTTAACAA GCGGCGCCAA GAAAACCAGG CGACCAGGAA TGCCCAGAAA GTCGAACCCT 
GACTGCGCCT GCGCCTGCCA ATCCGCAGTG ACGGAGCCGG CCGCGGAACC CAGCCAGCGA 
ACAGCTGTTG CCACGCACCA GGACTTGCCA CTGTCCACGT CGCAGTGGGG AAACGGAAGC 
GCAAACGCGA ACGCATCAAA GATCAATATC GATGGCCAGA TACTGCTCGA GCTGCTGAAG 
CACTCCTCGA ACACATTGCT GGAGACACTG CTCGGTGCTG GCAGTGCTGT GTCATCGAGT 
GCTCGCGACA CCCATCAGAT AGCCGAGGCA CAGCAGCAAA AGAACGCAAG GCTGCGCGAG 
GCCTTCAACA TATCGAGCTA CTTTGTGGAG GGCAGCAGCT TCGACAACGA TCGCAAAGCA 
AAAGAGGATC TGGCCAAAAG CGTGGCTCTA CAAAAGGAAC TGGACGCACA GCGCGAAAGC 
CTGGCAGCGG CGGCAGCGGC TGCAGCAGCT GCTGCAGGCA AGGACAAGGA TACGGGCAAG 
CGATATGCTT TGGTCCGTAC CCCATCCCGA GAGCGAGAGC GGGATGGAGA AGGCCATGTT 
GCCGCCGCCA GTGGCGATGA GCGCGAACAT GTCTCAAAGT CGGACAAGAA GAAGCGCAAG 
AAGCGCGCCA GAGAAAGCTC TGCCAGTCCT GAGCGCAAAA AGGACAAGAA AAAGAAGTCA 
AAGAAGCACA AGAAAGAGAG TAAGTCCAAA AAGAAGAAGT CCCGCAAGCG CAAGCACAGC 
GCCAGTTGCA CCGACAAGGA AACCGACAAG GACAGCGACG AGGAGGCGGC GGGCAGCAGC 
GATGAGGAGC GCGACCGCGA TCGTGAGCTA AAGAGCGCAC GCAAGAAAGC CAAGAAGGAC 
AAGAAAAAGC GCGACAAGAA GCTGAAAAAG AAGTCACGGG CCCGTGCCAG CTCCTCGGAC 
TCGGAGCCCT CCAACTCACC GTCGCGTAAT GAACGCAAAG GCAAGAAGTC GGGCAAATCC 
CGCGATCCAA CAGCTACCAA AGCTGAAGAC AATGGCACAT CGCGGGAGAA TCCCTTCCGG 
AGCAGGTCCC GCGAACGTCG CAGGGAATTA AAGCGGCCCA ACGAATCATC GGCCGACGGT 
CGAAGGGACA AGCGCGAGCG TAGCGATAGT CGCAGTCGAC GTCCCAAGTC CAATGAACGG 
GGGGCAACCA CCCGTACTCC ACCTCGCAAA GAGCCGGAAA GAAAGCGCGA ACGTTCTAAG 
GAGAGATTTC GATCAAAAGA AAGGCAACGA TCAAAAGAAA GACAGCGATC AAAGGAAAGA 
CTTCGTTCGA AGGAAAGACA ACGCTCGAAA GAAAGACAGC GATCAAAGGA ACGACCAAAA 
TCGAAGGAGA GACAGAGGTC GAGGGAAAGA CAGCGATCGA AGGAGAGGAT CCGCTCCAAA 
GAAAGACAGC GTTCAAAGGA AAGGCAACGC TCTAAAGAGA GGCAAAAGTC TAAGGATAGA 
CAGAGATCTA AGGATAGGCA GAGATCTAAG GATAGGCAGA GGTCAAAAGA GAGACAGAGG 
TCAAAGGAGA GGCAGCGATC AAAGGCGAGA CTTTATTCGA AAGAGAGACT GCGATCCAAG 
GAGAGGGCGC GATCAAAGGA TAAACCAGTA GTTGAAATCA AGCGCGACAG ATCAAAGGAT 
CGCCAGCGAT CAAAGGAGAG GAAACGATCG GTGGATAAGG CACCCTCGAA GGAGAGAGAT 
ATGTCCAAAG GTCGACAAAG ATCAAAGGAG AGACAGCGCT CCAAAGAAAG ACGACGTTCT 
AAGGATCGCG GTCAACAATC GAATGAGAAA AAGCGTTCCA AGGAGCGACA ACGATCAAAA 
GACCGTCAAC GATCCAAGGA GCGACAGGGA GTGCCAAACC TAGCCGTCTC TAAGGAACGT 
ACCCGGCGCC AGAGATCGCC ATCGACCTCG GCCAAAAATT CAACCGATAA TCGCAAGCCT 
ACTCGCAGTT CTAGATCACT TTCCCGTTCC CGCAAGCAGC AGTCGCCGGC CGCAGTTGTC 
GGGAGCAGCC GACGCAGTCA TTCCCCAGAG GTTCCACCCA AGAAGGCTGC GCCACCGGCA 
TTCAATCCCT TTAAGGCTGC AGAAGACACT GTCAACGATA TCCTGGGCAC CAAGTCGGTG 
ATTGTCGAAC TGGAGAAGAC CAAGCGCAAG CGGGCAGTCT CCAGCTCTAG CTCGGCATCG 
GGTAGCTCCA GTAGCAGCTC GTCTGCATCC CGGACACCTT CGCCCAAGCC CAAGCCTGGC 
AAACTGCGAC GACGCAGCAA AACATCCGAT CCTATCAAGA AGGAGAGGAG CCCCAAGCGA 
GATCGTCGTA CCCCGCGCCA GACCAGCGTG AAGCGTGAAC GTTCAAGCAC TCCACAAAAG 
CCCAAGCCAA AGGGAAAGGC GACCACACCC AGTCCGAAGC CAGCAAAGCG ACGCTCGCAC 
AGCTCAAGCT CTTCGGAACT GCGCTACTCG CCAGCAGAAC GACATCCAGA GCGCTATCAG 
GACATCATAC AAGACAAGAA GCGGCCATCG AAGGCACGCG ACCTTTCCAC AACGAAGCCC 
GCGCCCGTGG TGCGCCTGAG GGCCCAGAGC GATGACGGCA GCGATGCCGA TGCGAGTGGC 
TCCAATGTGG ATGCAGCAGC ACTTGACGAG TTCCAGCAGA GCAAGCAAGA GCGCGAGGAG 
CTGCAGGAGC TGCGTATGCT GGAGCAGCTC AAGTCGGGGA TCGCCGCCAA GGCAAAGCAG 
AAGATCAAGA CTATGGAGAA GAACGGAGAC GTCAGCAAGG ACTCTGCCAA GGAGGGCAGT 
GAAGTAAGTG GCAACGAACT GGTGACGGCT ACTAAACGTA ATTCGCTAAG TGAATTCCTT 
GTTGCTAACA ATGTAACCGC TTTGATTAAC CCATTGACAA CGACAACGAC TACGACAACA 
CCCCCGCCAC TCATGGCTGT GATCCTACCG CTGGCACAGC CACATCCGCA GCTACAGGAG 
CAGAAGCCGC AGCAGCAGCA TCAGTCTGTG GAGGAGCCAA ACCAAAAAAG AGACGACACC 
ACGACGCCGC CCATTTGCAA AGCGCCCCAA GTGGCTGCCA ATGGCGACGT TAAAAGTCCA 
CCAGCAACGA ACATGATAAC TAAGATCCAT CAAAGGCCTC CACAGCAGCA GCAGCAGCAT 
CAGCAGCAGC AGCAGCACCA GCACCAGCAG CAGTCGCATC AACACCAGCA TCCACACCAG 
CAACAGCATT CGCACCCACA CCAGACGAAG CGAATCTTTC ACAACCGAAC GCTGAACAAT 
AACCCTAACA GTAGACATAA TACTAACAAC AACAATAGCA ACAACAACAT TCATGCATGT 
GGTGGTGGTG TCCCTCATTC CAATAGCAAT CCCGGTATGC TGCCGTTCCT GGCCGGCACG 
CCGGGCACCT ACAACCGCAC AACGAACCGC CTTAACCACG GTCCGCTGCT GACGGCCACC 
CATTACAATA TATGCAAGAA CCATCAGAAC AGCATGCAAC AGCAAGCTCT GCTCGGTCGA 
CGCGACCACC AGCAGCAGCA TCTGGCGCAT GGTCTTGTTT ATAACCCTAG TCTCTTCGGC 
CTTGGGCAGC AGCAGGCTTT GCTGGCCGCT GGCGGCCAGC ATCATGGACG TCATGCCGTT 
GGGGGCCTTT TGGGCCTGGG CGGTGGTGGC GGACCTGCTG CGGGCCTGGC CTTGCTTAGT 
CATCCCCAGC ATGCGAGGAC CGGTAGCATT AGTTTCAACG CGGCTGCAGC GGCTGCGGCC 
GTGGCAGCCA ATAGTATAAG CTATCATCAT CATCACCAGC AACAGAAACC GAAAATTGTA 
ATAAAACCAT TCAAACTTCT GGATCCACAG CCCTTAGTTG CTGCACTCGC CGACAGCAGT 
CTGGTGGACG CCATCGTCTC CAAGGTGTCC ACGGCCACTG TGGCTGCAGC GGAGAGTGCA 
GCTCGTCGAA GTCGGAGTCG TTCACGCCGC AGCAGTCACA GCCGCAGCAA GCGGCACACG 
AGTGGCAGTC ATCATCATCA TCACTCCAGT AGTCGCTCAA GATCACGCTC CAGCTCCAGC 
AGCAGTGCCA GCGGCTCGCG CAGTTCTAGG TCCCGCTCTG GCTCGCAATC ATCTCGTTCC 
AGCTGCTCAT CCCAAAGCAG CTCGGGCAGC TCCTCCAGCA GCGGCTCAGG CACATCACAG 
TCTGGCTCAC GTTCCCCATC TATACCACGG CGTCGGGGCT CACCCAGCTT TCTAGACAGA 
CGCCGCATTA CGAGTGCTCG CAAGCGACCC ATTCCCTACC ACCACAAGGC AGCCGGTGGC 
ACTGAGGGGG GTGCTGCAGA GGCAGAGGAG GCCTCCAGCT GCTGCTCGAG CTGCTTCAGT 
CGGGCCAGCA CACCGGCCAC GCCCATCTGC ACTCCGCTGC GGAACAGTCG GAGCCCCTCG 
ATGGCCGCCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043095.1
CDS	225..5459
Translation	MYNGIGLTTARGSGTNGHVQRNCAFVRPGKKDKDYRSEDDIKKLDAQLNRPPNKEILDHDRKRKIEVKCLEFEEILEKQGRTPEDIKSQVDSFRQKLVGQGKTDAPKDEFGRVANTTTSTAMSAVGITSAASASAAASVATTTTTTATTLQVVKARKRRKRRKRAGHAIGAASTTTTITAAAATASHLGSPHTHTHPQAGSVSLPTANGGRKCKAQYFQHDNREYLAKYESFQLTAHHTASGAAALNYAAVAKKFKPLRPALTSGAKKTRRPGMPRKSNPDCACACQSAVTEPAAEPSQRTAVATHQDLPLSTSQWGNGSANANASKINIDGQILLELLKHSSNTLLETLLGAGSAVSSSARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEVSGNELVTATKRNSLSEFLVANNVTALINPLTTTTTTTTPPPLMAVILPLAQPHPQLQEQKPQQQHQSVEEPNQKRDDTTTPPICKAPQVAANGDVKSPPATNMITKIHQRPPQQQQQHQQQQQHQHQQQSHQHQHPHQQQHSHPHQTKRIFHNRTLNNNPNSRHNTNNNNSNNNIHACGGGVPHSNSNPGMLPFLAGTPGTYNRTTNRLNHGPLLTATHYNICKNHQNSMQQQALLGRRDHQQQHLAHGLVYNPSLFGLGQQQALLAAGGQHHGRHAVGGLLGLGGGGGPAAGLALLSHPQHARTGSISFNAAAAAAAVAANSISYHHHHQQQKPKIVIKPFKLLDPQPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_015187609.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant G (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187609.1

ORF Insert Sequence:

ATGTACAACG GCATTGGTTT GACAACCGCC CGCGGCTCCG GTACCAACGG ACATGTGCAG 
CGCAATTGTG CATTCGTTCG GCCGGGCAAA AAGGACAAGG ACTATCGCAG CGAAGATGAC 
ATCAAAAAGT TGGACGCCCA GCTGAATCGT CCGCCAAATA AGGAAATTCT TGACCATGAC 
CGCAAGCGCA AGATCGAAGT CAAGTGCTTG GAATTCGAGG AGATACTCGA GAAGCAGGGA 
CGCACACCGG AGGACATCAA ATCGCAAGTG GACTCGTTCC GTCAGAAGTT AGTGGGCCAA 
GGGAAGACAG ATGCACCAAA GGATGAATTT GGACGCGTAG CCAATACGAC CACCTCCACG 
GCTATGTCGG CGGTGGGGAT AACTTCAGCT GCTTCTGCTT CTGCTGCTGC CTCAGTGGCA 
ACCACAACAA CAACAACAGC CACCACATTG CAAGTGGTGA AAGCGAGAAA ACGCCGCAAG 
CGTCGGAAAC GCGCAGGTCA CGCAATTGGT GCCGCCTCCA CCACCACCAC CATCACAGCT 
GCAGCCGCCA CAGCCAGCCA CCTCGGCAGT CCCCACACCC ACACACACCC CCAAGCAGGA 
AGCGTTTCCC TACCGACAGC AAACGGTGGC AGGAAGTGCA AGGCCCAATA CTTCCAGCAC 
GACAATCGGG AGTACTTGGC AAAGTACGAG AGCTTTCAAC TCACGGCGCA CCACACGGCA 
TCGGGCGCAG CTGCGCTGAA CTATGCGGCG GTGGCGAAAA AGTTCAAGCC CTTGCGGCCG 
GCGTTAACAA GCGGCGCCAA GAAAACCAGG CGACCAGGAA TGCCCAGAAA GTCGAACCCT 
GACTGCGCCT GCGCCTGCCA ATCCGCAGTG ACGGAGCCGG CCGCGGAACC CAGCCAGCGA 
ACAGCTGTTG CCACGCACCA GGACTTGCCA CTGTCCACGT CGCAGTGGGG AAACGGAAGC 
GCAAACGCGA ACGCATCAAA GATCAATATC GATGGCCAGA TACTGCTCGA GCTGCTGAAG 
CACTCCTCGA ACACATTGCT GGAGACACTG CTCGGTGCTG GCAGTGCTGT GTCATCGAGT 
GCTCGCGACA CCCATCAGAT AGCCGAGGCA CAGCAGCAAA AGAACGCAAG GCTGCGCGAG 
GCCTTCAACA TATCGAGCTA CTTTGTGGAG GGCAGCAGCT TCGACAACGA TCGCAAAGCA 
AAAGAGGATC TGGCCAAAAG CGTGGCTCTA CAAAAGGAAC TGGACGCACA GCGCGAAAGC 
CTGGCAGCGG CGGCAGCGGC TGCAGCAGCT GCTGCAGGCA AGGACAAGGA TACGGGCAAG 
CGATATGCTT TGGTCCGTAC CCCATCCCGA GAGCGAGAGC GGGATGGAGA AGGCCATGTT 
GCCGCCGCCA GTGGCGATGA GCGCGAACAT GTCTCAAAGT CGGACAAGAA GAAGCGCAAG 
AAGCGCGCCA GAGAAAGCTC TGCCAGTCCT GAGCGCAAAA AGGACAAGAA AAAGAAGTCA 
AAGAAGCACA AGAAAGAGAG TAAGTCCAAA AAGAAGAAGT CCCGCAAGCG CAAGCACAGC 
GCCAGTTGCA CCGACAAGGA AACCGACAAG GACAGCGACG AGGAGGCGGC GGGCAGCAGC 
GATGAGGAGC GCGACCGCGA TCGTGAGCTA AAGAGCGCAC GCAAGAAAGC CAAGAAGGAC 
AAGAAAAAGC GCGACAAGAA GCTGAAAAAG AAGTCACGGG CCCGTGCCAG CTCCTCGGAC 
TCGGAGCCCT CCAACTCACC GTCGCGTAAT GAACGCAAAG GCAAGAAGTC GGGCAAATCC 
CGCGATCCAA CAGCTACCAA AGCTGAAGAC AATGGCACAT CGCGGGAGAA TCCCTTCCGG 
AGCAGGTCCC GCGAACGTCG CAGGGAATTA AAGCGGCCCA ACGAATCATC GGCCGACGGT 
CGAAGGGACA AGCGCGAGCG TAGCGATAGT CGCAGTCGAC GTCCCAAGTC CAATGAACGG 
GGGGCAACCA CCCGTACTCC ACCTCGCAAA GAGCCGGAAA GAAAGCGCGA ACGTTCTAAG 
GAGAGATTTC GATCAAAAGA AAGGCAACGA TCAAAAGAAA GACAGCGATC AAAGGAAAGA 
CTTCGTTCGA AGGAAAGACA ACGCTCGAAA GAAAGACAGC GATCAAAGGA ACGACCAAAA 
TCGAAGGAGA GACAGAGGTC GAGGGAAAGA CAGCGATCGA AGGAGAGGAT CCGCTCCAAA 
GAAAGACAGC GTTCAAAGGA AAGGCAACGC TCTAAAGAGA GGCAAAAGTC TAAGGATAGA 
CAGAGATCTA AGGATAGGCA GAGATCTAAG GATAGGCAGA GGTCAAAAGA GAGACAGAGG 
TCAAAGGAGA GGCAGCGATC AAAGGCGAGA CTTTATTCGA AAGAGAGACT GCGATCCAAG 
GAGAGGGCGC GATCAAAGGA TAAACCAGTA GTTGAAATCA AGCGCGACAG ATCAAAGGAT 
CGCCAGCGAT CAAAGGAGAG GAAACGATCG GTGGATAAGG CACCCTCGAA GGAGAGAGAT 
ATGTCCAAAG GTCGACAAAG ATCAAAGGAG AGACAGCGCT CCAAAGAAAG ACGACGTTCT 
AAGGATCGCG GTCAACAATC GAATGAGAAA AAGCGTTCCA AGGAGCGACA ACGATCAAAA 
GACCGTCAAC GATCCAAGGA GCGACAGGGA GTGCCAAACC TAGCCGTCTC TAAGGAACGT 
ACCCGGCGCC AGAGATCGCC ATCGACCTCG GCCAAAAATT CAACCGATAA TCGCAAGCCT 
ACTCGCAGTT CTAGATCACT TTCCCGTTCC CGCAAGCAGC AGTCGCCGGC CGCAGTTGTC 
GGGAGCAGCC GACGCAGTCA TTCCCCAGAG GTTCCACCCA AGAAGGCTGC GCCACCGGCA 
TTCAATCCCT TTAAGGCTGC AGAAGACACT GTCAACGATA TCCTGGGCAC CAAGTCGGTG 
ATTGTCGAAC TGGAGAAGAC CAAGCGCAAG CGGGCAGTCT CCAGCTCTAG CTCGGCATCG 
GGTAGCTCCA GTAGCAGCTC GTCTGCATCC CGGACACCTT CGCCCAAGCC CAAGCCTGGC 
AAACTGCGAC GACGCAGCAA AACATCCGAT CCTATCAAGA AGGAGAGGAG CCCCAAGCGA 
GATCGTCGTA CCCCGCGCCA GACCAGCGTG AAGCGTGAAC GTTCAAGCAC TCCACAAAAG 
CCCAAGCCAA AGGGAAAGGC GACCACACCC AGTCCGAAGC CAGCAAAGCG ACGCTCGCAC 
AGCTCAAGCT CTTCGGAACT GCGCTACTCG CCAGCAGAAC GACATCCAGA GCGCTATCAG 
GACATCATAC AAGACAAGAA GCGGCCATCG AAGGCACGCG ACCTTTCCAC AACGAAGCCC 
GCGCCCGTGG TGCGCCTGAG GGCCCAGAGC GATGACGGCA GCGATGCCGA TGCGAGTGGC 
TCCAATGTGG ATGCAGCAGC ACTTGACGAG TTCCAGCAGA GCAAGCAAGA GCGCGAGGAG 
CTGCAGGAGC TGCGTATGCT GGAGCAGCTC AAGTCGGGGA TCGCCGCCAA GGCAAAGCAG 
AAGATCAAGA CTATGGAGAA GAACGGAGAC GTCAGCAAGG ACTCTGCCAA GGAGGGCAGT 
GAAGTAAGTG GCAACGAACT GGTGACGGCT ACTAAACGTA ATTCGCTAAG TGAATTCCTT 
GTTGCTAACA ATGTAACCGC TTTGATTAAC CCATTGACAA CGACAACGAC TACGACAACA 
CCCCCGCCAC TCATGGCTGT GATCCTACCG CTGGCACAGC CACATCCGCA GCTACAGGAG 
CAGAAGCCGC AGCAGCAGCA TCAGTCTGTG GAGGAGCCAA ACCAAAAAAG AGACGACACC 
ACGACGCCGC CCATTTGCAA AGCGCCCCAA GTGGCTGCCA ATGGCGACGT TAAAAGTCCA 
CCAGCAACGA ACATGATAAC TAAGATCCAT CAAAGGCCTC CACAGCAGCA GCAGCAGCAT 
CAGCAGCAGC AGCAGCACCA GCACCAGCAG CAGTCGCATC AACACCAGCA TCCACACCAG 
CAACAGCATT CGCACCCACA CCAGACGAAG CGAATCTTTC ACAACCGAAC GCTGAACAAT 
AACCCTAACA GTAGACATAA TACTAACAAC AACAATAGCA ACAACAACAT TCATGCATGT 
GGTGGTGGTG TCCCTCATTC CAATAGCAAT CCCGGTATGC TGCCGTTCCT GGCCGGCACG 
CCGGGCACCT ACAACCGCAC AACGAACCGC CTTAACCACG GTCCGCTGCT GACGGCCACC 
CATTACAATA TATGCAAGAA CCATCAGAAC AGCATGCAAC AGCAAGCTCT GCTCGGTCGA 
CGCGACCACC AGCAGCAGCA TCTGGCGCAT GGTCTTGTTT ATAACCCTAG TCTCTTCGGC 
CTTGGGCAGC AGCAGGCTTT GCTGGCCGCT GGCGGCCAGC ATCATGGACG TCATGCCGTT 
GGGGGCCTTT TGGGCCTGGG CGGTGGTGGC GGACCTGCTG CGGGCCTGGC CTTGCTTAGT 
CATCCCCAGC ATGCGAGGAC CGGTAGCATT AGTTTCAACG CGGCTGCAGC GGCTGCGGCC 
GTGGCAGCCA ATAGTATAAG CTATCATCAT CATCACCAGC AACAGAAACC GAAAATTGTA 
ATAAAACCAT TCAAACTTCT GGATCCACAG CCCTTAGTTG CTGCACTCGC CGACAGCAGT 
CTGGTGGACG CCATCGTCTC CAAGGTGTCC ACGGCCACTG TGGCTGCAGC GGAGAGTGCA 
GCTCGTCGAA GTCGGAGTCG TTCACGCCGC AGCAGTCACA GCCGCAGCAA GCGGCACACG 
AGTGGCAGTC ATCATCATCA TCACTCCAGT AGTCGCTCAA GATCACGCTC CAGCTCCAGC 
AGCAGTGCCA GCGGCTCGCG CAGTTCTAGG TCCCGCTCTG GCTCGCAATC ATCTCGTTCC 
AGCTGCTCAT CCCAAAGCAG CTCGGGCAGC TCCTCCAGCA GCGGCTCAGG CACATCACAG 
TCTGGCTCAC GTTCCCCATC TATACCACGG CGTCGGGGCT CACCCAGCTT TCTAGACAGA 
CGCCGCATTA CGAGTGCTCG CAAGCGACCC ATTCCCTACC ACCACAAGGC AGCCGGTGGC 
ACTGAGGGGG GTGCTGCAGA GGCAGAGGAG GCCTCCAGCT GCTGCTCGAG CTGCTTCAGT 
CGGGCCAGCA CACCGGCCAC GCCCATCTGC ACTCCGCTGC GGAACAGTCG GAGCCCCTCG 
ATGGCCGCCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA23474 ( XM_015187611.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015043097.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant K (Dpse\GA23474), mRNA.

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CloneID	ODe26412
Clone ID Related Accession (Same CDS sequence)	XM_015187611.1
Accession Version	XM_015187611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform K
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTCCT CGGAAATCTA CACCAATACG ACCACCTCCA CGGCTATGTC GGCGGTGGGG 
ATAACTTCAG CTGCTTCTGC TTCTGCTGCT GCCTCAGTGG CAACCACAAC AACAACAACA 
GCCACCACAT TGCAAGTGGT GAAAGCGAGA AAACGCCGCA AGCGTCGGAA ACGCGCAGGT 
CACGCAATTG GTGCCGCCTC CACCACCACC ACCATCACAG CTGCAGCCGC CACAGCCAGC 
CACCTCGGCA GTCCCCACAC CCACACACAC CCCCAAGCAG GAAGCGTTTC CCTACCGACA 
GCAAACGGTG GCAGGAAGTG CAAGGCCCAA TACTTCCAGC ACGACAATCG GGAGTACTTG 
GCAAAGTACG AGAGCTTTCA ACTCACGGCG CACCACACGG CATCGGGCGC AGCTGCGCTG 
AACTATGCGG CGGTGGCGAA AAAGTTCAAG CCCTTGCGGC CGGCGTTAAC AAGCGGCGCC 
AAGAAAACCA GGCGACCAGG AATGCCCAGA AAGTCGAACC CTGACTGCGC CTGCGCCTGC 
CAATCCGCAG TGACGGAGCC GGCCGCGGAA CCCAGCCAGC GAACAGCTGT TGCCACGCAC 
CAGGACTTGC CACTGTCCAC GTCGCAGTGG GGAAACGGAA GCGCAAACGC GAACGCATCA 
AAGATCAATA TCGATGGCCA GATACTGCTC GAGCTGCTGA AGCACTCCTC GAACACATTG 
CTGGAGACAC TGCTCGGTGC TGGCAGTGCT GTGTCATCGA GTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAAGTAAG TGGCAACGAA CTGGTGACGG CTACTAAACG TAATTCGCTA 
AGTGAATTCC TTGTTGCTAA CAATGTAACC GCTTTGATTA ACCCATTGAC AACGACAACG 
ACTACGACAA CACCCCCGCC ACTCATGGCT GTGATCCTAC CGCTGGCACA GCCACATCCG 
CAGCTACAGG AGCAGAAGCC GCAGCAGCAG CATCAGTCTG TGGAGGAGCC AAACCAAAAA 
AGAGACGACA CCACGACGCC GCCCATTTGC AAAGCGCCCC AAGTGGCTGC CAATGGCGAC 
GTTAAAAGTC CACCAGCAAC GAACATGATA ACTAAGATCC ATCAAAGGCC TCCACAGCAG 
CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC CAGCACCAGC AGCAGTCGCA TCAACACCAG 
CATCCACACC AGCAACAGCA TTCGCACCCA CACCAGACGA AGCGAATCTT TCACAACCGA 
ACGCTGAACA ATAACCCTAA CAGTAGACAT AATACTAACA ACAACAATAG CAACAACAAC 
ATTCATGCAT GTGGTGGTGG TGTCCCTCAT TCCAATAGCA ATCCCGGTAT GCTGCCGTTC 
CTGGCCGGCA CGCCGGGCAC CTACAACCGC ACAACGAACC GCCTTAACCA CGGTCCGCTG 
CTGACGGCCA CCCATTACAA TATATGCAAG AACCATCAGA ACAGCATGCA ACAGCAAGCT 
CTGCTCGGTC GACGCGACCA CCAGCAGCAG CATCTGGCGC ATGGTCTTGT TTATAACCCT 
AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT TTGCTGGCCG CTGGCGGCCA GCATCATGGA 
CGTCATGCCG TTGGGGGCCT TTTGGGCCTG GGCGGTGGTG GCGGACCTGC TGCGGGCCTG 
GCCTTGCTTA GTCATCCCCA GCATGCGAGG ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA 
GCGGCTGCGG CCGTGGCAGC CAATAGTATA AGCTATCATC ATCATCACCA GCAACAGAAA 
CCGAAAATTG TAATAAAACC ATTCAAACTT CTGGATCCAC AGCCCTTAGT TGCTGCACTC 
GCCGACAGCA GTCTGGTGGA CGCCATCGTC TCCAAGGTGT CCACGGCCAC TGTGGCTGCA 
GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT CGTTCACGCC GCAGCAGTCA CAGCCGCAGC 
AAGCGGCACA CGAGTGGCAG TCATCATCAT CATCACTCCA GTAGTCGCTC AAGATCACGC 
TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA 
TCATCTCGTT CCAGCTGCTC ATCCCAAAGC AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA 
GGCACATCAC AGTCTGGCTC ACGTTCCCCA TCTATACCAC GGCGTCGGGG CTCACCCAGC 
TTTCTAGACA GACGCCGCAT TACGAGTGCT CGCAAGCGAC CCATTCCCTA CCACCACAAG 
GCAGCCGGTG GCACTGAGGG GGGTGCTGCA GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG 
AGCTGCTTCA GTCGGGCCAG CACACCGGCC ACGCCCATCT GCACTCCGCT GCGGAACAGT 
CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015043097.1
CDS	28..4974
Translation	MLSSEIYTNTTTSTAMSAVGITSAASASAAASVATTTTTTATTLQVVKARKRRKRRKRAGHAIGAASTTTTITAAAATASHLGSPHTHTHPQAGSVSLPTANGGRKCKAQYFQHDNREYLAKYESFQLTAHHTASGAAALNYAAVAKKFKPLRPALTSGAKKTRRPGMPRKSNPDCACACQSAVTEPAAEPSQRTAVATHQDLPLSTSQWGNGSANANASKINIDGQILLELLKHSSNTLLETLLGAGSAVSSSARDTHQIAEAQQQKNARLREAFNISSYFVEGSSFDNDRKAKEDLAKSVALQKELDAQRESLAAAAAAAAAAAGKDKDTGKRYALVRTPSRERERDGEGHVAAASGDEREHVSKSDKKKRKKRARESSASPERKKDKKKKSKKHKKESKSKKKKSRKRKHSASCTDKETDKDSDEEAAGSSDEERDRDRELKSARKKAKKDKDSFQWLSCSFKKRDKKLKKKSRARASSSDSEPSNSPSRNERKGKKSGKSRDPTATKAEDNGTSRENPFRSRSRERRRELKRPNESSADGRRDKRERSDSRSRRPKSNERGATTRTPPRKEPERKRERSKERFRSKERQRSKERQRSKERLRSKERQRSKERQRSKERPKSKERQRSRERQRSKERIRSKERQRSKERQRSKERQKSKDRQRSKDRQRSKDRQRSKERQRSKERQRSKARLYSKERLRSKERARSKDKPVVEIKRDRSKDRQRSKERKRSVDKAPSKERDMSKGRQRSKERQRSKERRRSKDRGQQSNEKKRSKERQRSKDRQRSKERQGVPNLAVSKERTRRQRSPSTSAKNSTDNRKPTRSSRSLSRSRKQQSPAAVVGSSRRSHSPEVPPKKAAPPAFNPFKAAEDTVNDILGTKSVIVELEKTKRKRAVSSSSSASGSSSSSSSASRTPSPKPKPGKLRRRSKTSDPIKKERSPKRDRRTPRQTSVKRERSSTPQKPKPKGKATTPSPKPAKRRSHSSSSSELRYSPAERHPERYQDIIQDKKRPSKARDLSTTKPAPVVRLRAQSDDGSDADASGSNVDAAALDEFQQSKQEREELQELRMLEQLKSGIAAKAKQKIKTMEKNGDVSKDSAKEGSEVSGNELVTATKRNSLSEFLVANNVTALINPLTTTTTTTTPPPLMAVILPLAQPHPQLQEQKPQQQHQSVEEPNQKRDDTTTPPICKAPQVAANGDVKSPPATNMITKIHQRPPQQQQQHQQQQQHQHQQQSHQHQHPHQQQHSHPHQTKRIFHNRTLNNNPNSRHNTNNNNSNNNIHACGGGVPHSNSNPGMLPFLAGTPGTYNRTTNRLNHGPLLTATHYNICKNHQNSMQQQALLGRRDHQQQHLAHGLVYNPSLFGLGQQQALLAAGGQHHGRHAVGGLLGLGGGGGPAAGLALLSHPQHARTGSISFNAAAAAAAVAANSISYHHHHQQQKPKIVIKPFKLLDPQPLVAALADSSLVDAIVSKVSTATVAAAESAARRSRSRSRRSSHSRSKRHTSGSHHHHHSSSRSRSRSSSSSSASGSRSSRSRSGSQSSRSSCSSQSSSGSSSSSGSGTSQSGSRSPSIPRRRGSPSFLDRRRITSARKRPIPYHHKAAGGTEGGAAEAEEASSCCSSCFSRASTPATPICTPLRNSRSPSMAAF

Target ORF information:

RefSeq Version	XM_015187611.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant K (Dpse\GA23474), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187611.1

ORF Insert Sequence:

ATGCTCTCCT CGGAAATCTA CACCAATACG ACCACCTCCA CGGCTATGTC GGCGGTGGGG 
ATAACTTCAG CTGCTTCTGC TTCTGCTGCT GCCTCAGTGG CAACCACAAC AACAACAACA 
GCCACCACAT TGCAAGTGGT GAAAGCGAGA AAACGCCGCA AGCGTCGGAA ACGCGCAGGT 
CACGCAATTG GTGCCGCCTC CACCACCACC ACCATCACAG CTGCAGCCGC CACAGCCAGC 
CACCTCGGCA GTCCCCACAC CCACACACAC CCCCAAGCAG GAAGCGTTTC CCTACCGACA 
GCAAACGGTG GCAGGAAGTG CAAGGCCCAA TACTTCCAGC ACGACAATCG GGAGTACTTG 
GCAAAGTACG AGAGCTTTCA ACTCACGGCG CACCACACGG CATCGGGCGC AGCTGCGCTG 
AACTATGCGG CGGTGGCGAA AAAGTTCAAG CCCTTGCGGC CGGCGTTAAC AAGCGGCGCC 
AAGAAAACCA GGCGACCAGG AATGCCCAGA AAGTCGAACC CTGACTGCGC CTGCGCCTGC 
CAATCCGCAG TGACGGAGCC GGCCGCGGAA CCCAGCCAGC GAACAGCTGT TGCCACGCAC 
CAGGACTTGC CACTGTCCAC GTCGCAGTGG GGAAACGGAA GCGCAAACGC GAACGCATCA 
AAGATCAATA TCGATGGCCA GATACTGCTC GAGCTGCTGA AGCACTCCTC GAACACATTG 
CTGGAGACAC TGCTCGGTGC TGGCAGTGCT GTGTCATCGA GTGCTCGCGA CACCCATCAG 
ATAGCCGAGG CACAGCAGCA AAAGAACGCA AGGCTGCGCG AGGCCTTCAA CATATCGAGC 
TACTTTGTGG AGGGCAGCAG CTTCGACAAC GATCGCAAAG CAAAAGAGGA TCTGGCCAAA 
AGCGTGGCTC TACAAAAGGA ACTGGACGCA CAGCGCGAAA GCCTGGCAGC GGCGGCAGCG 
GCTGCAGCAG CTGCTGCAGG CAAGGACAAG GATACGGGCA AGCGATATGC TTTGGTCCGT 
ACCCCATCCC GAGAGCGAGA GCGGGATGGA GAAGGCCATG TTGCCGCCGC CAGTGGCGAT 
GAGCGCGAAC ATGTCTCAAA GTCGGACAAG AAGAAGCGCA AGAAGCGCGC CAGAGAAAGC 
TCTGCCAGTC CTGAGCGCAA AAAGGACAAG AAAAAGAAGT CAAAGAAGCA CAAGAAAGAG 
AGTAAGTCCA AAAAGAAGAA GTCCCGCAAG CGCAAGCACA GCGCCAGTTG CACCGACAAG 
GAAACCGACA AGGACAGCGA CGAGGAGGCG GCGGGCAGCA GCGATGAGGA GCGCGACCGC 
GATCGTGAGC TAAAGAGCGC ACGCAAGAAA GCCAAGAAGG ACAAGGATAG CTTTCAATGG 
CTCTCTTGTA GTTTTAAAAA GCGCGACAAG AAGCTGAAAA AGAAGTCACG GGCCCGTGCC 
AGCTCCTCGG ACTCGGAGCC CTCCAACTCA CCGTCGCGTA ATGAACGCAA AGGCAAGAAG 
TCGGGCAAAT CCCGCGATCC AACAGCTACC AAAGCTGAAG ACAATGGCAC ATCGCGGGAG 
AATCCCTTCC GGAGCAGGTC CCGCGAACGT CGCAGGGAAT TAAAGCGGCC CAACGAATCA 
TCGGCCGACG GTCGAAGGGA CAAGCGCGAG CGTAGCGATA GTCGCAGTCG ACGTCCCAAG 
TCCAATGAAC GGGGGGCAAC CACCCGTACT CCACCTCGCA AAGAGCCGGA AAGAAAGCGC 
GAACGTTCTA AGGAGAGATT TCGATCAAAA GAAAGGCAAC GATCAAAAGA AAGACAGCGA 
TCAAAGGAAA GACTTCGTTC GAAGGAAAGA CAACGCTCGA AAGAAAGACA GCGATCAAAG 
GAACGACCAA AATCGAAGGA GAGACAGAGG TCGAGGGAAA GACAGCGATC GAAGGAGAGG 
ATCCGCTCCA AAGAAAGACA GCGTTCAAAG GAAAGGCAAC GCTCTAAAGA GAGGCAAAAG 
TCTAAGGATA GACAGAGATC TAAGGATAGG CAGAGATCTA AGGATAGGCA GAGGTCAAAA 
GAGAGACAGA GGTCAAAGGA GAGGCAGCGA TCAAAGGCGA GACTTTATTC GAAAGAGAGA 
CTGCGATCCA AGGAGAGGGC GCGATCAAAG GATAAACCAG TAGTTGAAAT CAAGCGCGAC 
AGATCAAAGG ATCGCCAGCG ATCAAAGGAG AGGAAACGAT CGGTGGATAA GGCACCCTCG 
AAGGAGAGAG ATATGTCCAA AGGTCGACAA AGATCAAAGG AGAGACAGCG CTCCAAAGAA 
AGACGACGTT CTAAGGATCG CGGTCAACAA TCGAATGAGA AAAAGCGTTC CAAGGAGCGA 
CAACGATCAA AAGACCGTCA ACGATCCAAG GAGCGACAGG GAGTGCCAAA CCTAGCCGTC 
TCTAAGGAAC GTACCCGGCG CCAGAGATCG CCATCGACCT CGGCCAAAAA TTCAACCGAT 
AATCGCAAGC CTACTCGCAG TTCTAGATCA CTTTCCCGTT CCCGCAAGCA GCAGTCGCCG 
GCCGCAGTTG TCGGGAGCAG CCGACGCAGT CATTCCCCAG AGGTTCCACC CAAGAAGGCT 
GCGCCACCGG CATTCAATCC CTTTAAGGCT GCAGAAGACA CTGTCAACGA TATCCTGGGC 
ACCAAGTCGG TGATTGTCGA ACTGGAGAAG ACCAAGCGCA AGCGGGCAGT CTCCAGCTCT 
AGCTCGGCAT CGGGTAGCTC CAGTAGCAGC TCGTCTGCAT CCCGGACACC TTCGCCCAAG 
CCCAAGCCTG GCAAACTGCG ACGACGCAGC AAAACATCCG ATCCTATCAA GAAGGAGAGG 
AGCCCCAAGC GAGATCGTCG TACCCCGCGC CAGACCAGCG TGAAGCGTGA ACGTTCAAGC 
ACTCCACAAA AGCCCAAGCC AAAGGGAAAG GCGACCACAC CCAGTCCGAA GCCAGCAAAG 
CGACGCTCGC ACAGCTCAAG CTCTTCGGAA CTGCGCTACT CGCCAGCAGA ACGACATCCA 
GAGCGCTATC AGGACATCAT ACAAGACAAG AAGCGGCCAT CGAAGGCACG CGACCTTTCC 
ACAACGAAGC CCGCGCCCGT GGTGCGCCTG AGGGCCCAGA GCGATGACGG CAGCGATGCC 
GATGCGAGTG GCTCCAATGT GGATGCAGCA GCACTTGACG AGTTCCAGCA GAGCAAGCAA 
GAGCGCGAGG AGCTGCAGGA GCTGCGTATG CTGGAGCAGC TCAAGTCGGG GATCGCCGCC 
AAGGCAAAGC AGAAGATCAA GACTATGGAG AAGAACGGAG ACGTCAGCAA GGACTCTGCC 
AAGGAGGGCA GTGAAGTAAG TGGCAACGAA CTGGTGACGG CTACTAAACG TAATTCGCTA 
AGTGAATTCC TTGTTGCTAA CAATGTAACC GCTTTGATTA ACCCATTGAC AACGACAACG 
ACTACGACAA CACCCCCGCC ACTCATGGCT GTGATCCTAC CGCTGGCACA GCCACATCCG 
CAGCTACAGG AGCAGAAGCC GCAGCAGCAG CATCAGTCTG TGGAGGAGCC AAACCAAAAA 
AGAGACGACA CCACGACGCC GCCCATTTGC AAAGCGCCCC AAGTGGCTGC CAATGGCGAC 
GTTAAAAGTC CACCAGCAAC GAACATGATA ACTAAGATCC ATCAAAGGCC TCCACAGCAG 
CAGCAGCAGC ATCAGCAGCA GCAGCAGCAC CAGCACCAGC AGCAGTCGCA TCAACACCAG 
CATCCACACC AGCAACAGCA TTCGCACCCA CACCAGACGA AGCGAATCTT TCACAACCGA 
ACGCTGAACA ATAACCCTAA CAGTAGACAT AATACTAACA ACAACAATAG CAACAACAAC 
ATTCATGCAT GTGGTGGTGG TGTCCCTCAT TCCAATAGCA ATCCCGGTAT GCTGCCGTTC 
CTGGCCGGCA CGCCGGGCAC CTACAACCGC ACAACGAACC GCCTTAACCA CGGTCCGCTG 
CTGACGGCCA CCCATTACAA TATATGCAAG AACCATCAGA ACAGCATGCA ACAGCAAGCT 
CTGCTCGGTC GACGCGACCA CCAGCAGCAG CATCTGGCGC ATGGTCTTGT TTATAACCCT 
AGTCTCTTCG GCCTTGGGCA GCAGCAGGCT TTGCTGGCCG CTGGCGGCCA GCATCATGGA 
CGTCATGCCG TTGGGGGCCT TTTGGGCCTG GGCGGTGGTG GCGGACCTGC TGCGGGCCTG 
GCCTTGCTTA GTCATCCCCA GCATGCGAGG ACCGGTAGCA TTAGTTTCAA CGCGGCTGCA 
GCGGCTGCGG CCGTGGCAGC CAATAGTATA AGCTATCATC ATCATCACCA GCAACAGAAA 
CCGAAAATTG TAATAAAACC ATTCAAACTT CTGGATCCAC AGCCCTTAGT TGCTGCACTC 
GCCGACAGCA GTCTGGTGGA CGCCATCGTC TCCAAGGTGT CCACGGCCAC TGTGGCTGCA 
GCGGAGAGTG CAGCTCGTCG AAGTCGGAGT CGTTCACGCC GCAGCAGTCA CAGCCGCAGC 
AAGCGGCACA CGAGTGGCAG TCATCATCAT CATCACTCCA GTAGTCGCTC AAGATCACGC 
TCCAGCTCCA GCAGCAGTGC CAGCGGCTCG CGCAGTTCTA GGTCCCGCTC TGGCTCGCAA 
TCATCTCGTT CCAGCTGCTC ATCCCAAAGC AGCTCGGGCA GCTCCTCCAG CAGCGGCTCA 
GGCACATCAC AGTCTGGCTC ACGTTCCCCA TCTATACCAC GGCGTCGGGG CTCACCCAGC 
TTTCTAGACA GACGCCGCAT TACGAGTGCT CGCAAGCGAC CCATTCCCTA CCACCACAAG 
GCAGCCGGTG GCACTGAGGG GGGTGCTGCA GAGGCAGAGG AGGCCTCCAG CTGCTGCTCG 
AGCTGCTTCA GTCGGGCCAG CACACCGGCC ACGCCCATCT GCACTCCGCT GCGGAACAGT 
CGGAGCCCCT CGATGGCCGC CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA23474 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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