Dpse\GA10453 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10453
Entrez Gene ID	4816176
Full Name	GA10453 gene product from transcript GA10453-RC
Synonyms	GA10453,dpse_GLEANR_7381
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015181414.1	XP_015036900.1	uncharacterized protein, isoform B
XM_001355708.3	XP_001355744.3	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10453 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10453 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe18763	XM_001355708.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10453), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODe18827	XM_015181414.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10453), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10453 ( XM_001355708.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355744.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10453), mRNA.

Price & Availability↑

CloneID	ODe18763
Clone ID Related Accession (Same CDS sequence)	XM_001355708.3
Accession Version	XM_001355708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). On Dec 22, 2015 this sequence version replaced XM_001355708.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCACAGC ATAATCCACA TTCCCATCCG CACTACCATC AGACACAACA CCACAACCAT 
CAGCAGTTGC AGCAGCAGCA ACAACAACAG CAGCCACACA GCAATTGGTA CTCACATGTT 
GCTTCCTACC CCCCACCGCC ACCCCAGTAC CATGCGTCGG CTACCTCCGC ATTTGCCGCT 
ACCTCCACAC CGTTATCGAA CGATATATAT AGGAACTTTG GTCGTACCGA CAACAATGAT 
TCCCCCAGCT TTGGCCACCG GAATTCGTCA AACACCAACT TCGGCGACAA CAATGGGCGT 
TACTTCAATG ATGCTCCACA AGATTATTAT GTGGCCAACT TTATTGACAA TTATCGCAGT 
GGCCCGAGGT CGCAAAACTT TGGGGATCAG CGTTCCTCCT ATATGGGACA TGATATAATG 
TCCGGAACTT ATTATCCGTA CATAAAGAGC GAACCCATGG AGTCAGCGTA TCAGCAGCCA 
CAGAATCCGA TGGCCCCGCC CCCAGCGCCA GATATGATAA TAAAATCGGA ACCCATGGAT 
GAACATCCCT ACAAGTCAAA CTATATTGAT GACAATACGC CCTTTGCTGA TTTTAACAAG 
TTCAACGAAT TCAGCGGCGA TATGCTAAGC CCCAAAGTCG AGCTAACCGT CAAGGATGAG 
ACTTACGGAA AGACTTCCAG CAGCAATAGC AGCAGCAGCT TTGCACGTCG AAAAGCCCAG 
CAACAGCAAA CGACAGATCG TTCGGCGGAG AGTCTGCCCA TCTGCCAGCG CTGCAAGGAG 
GTCTTCTTCA AAAAGCAATC CTATCTGAGG CATGTGGCCG AGAGCAGTTG TGGCATTCAG 
GAGTACGATT TCAAGTGCAA CATATGCCCC ATGTCCTTCA TGACCACTGA AGAGCTGCAG 
CGCCACAAGC AACTGCATCG TGCAGACAAG TTCTTCTGCC ACAAATACTG CGGCAAGCAT 
TTCGATACGA TAGCCGAGTG CGAATCGCAC GAGTACATGC AGCACGAGTA TGAGAGATTT 
GTTTGTAATA TGTGCTCTGG AACCTTTGCC ACGCGGGAAC AGCTGTATGC CCACTTGCCG 
CAACACAAGT TTCAGCAGCG TTACGACTGT CCCATCTGCC GTTTGTGGTA TCAAACAGCC 
GTCGAATTGC ATGAGCATCG ACTGTCGGCT CCATACTTTT GCGGCAAGTA CTATACCAGT 
CAACAGCAGC AGCTTGCTAC GAGCCAGGGG AATTACAAGC TGCAGGACTG CCATATGGCC 
ACCATGGAAA TACCCACTGC ACCACTGCAC AAGGCAACGC CTTCCAATCC CTCAGCCTTG 
CCAGCCACAG CTGCCTTGAG CTCTCTGTTG CAACAGCGCC AGGCAAATGC CGATGGGACT 
GCGGCCATGT TTGCTGCGGC CTCCTCTTCC TCCGCCTCGC TGAAGAGCGA GGTGAGCGTG 
AAGCTAGAGC GTAGCTACAG CAACTCCACC AGCGAGTCCT CTTACAGCCA TCAGGACAAC 
AGCAGCTACA ACAATGCCTA TGGCAGCGAC AACTCCATCC ATGGCGGAGC ACTGGCCGGA 
CCACAGGCTC ACTCCTCAAC GCTGGACGAC TCCGAGGATG CCCTGTGTTG TGTGCCCCTG 
TGCGGCGTCA GAAAAAGCAC CAGTCCAACG CTGCAGTTCT TTACGTTTCC AAAGGATGAA 
AAGTATCTGA ATCAGTGGCT ACACAACCTC AAGATGTTCC ACATACCAGC GGCGAGCTAT 
GCGACATTTC GCATCTGTAG CATGCACTTC CCGAAGCGTT GTATCAATCG GTATTCGCTG 
TGCTATTGGG CGGTGCCCAC CTTCAATCTG GGGCACGACG ATGTGGCCAA TCTGTACCAG 
AACCGTGAGT TAACCAACAC TTTTACCACT GGAGAGGTGG CACGCTGCAG CATGCCGCAT 
TGCACCAGCC AGCGGGGGGA GAGCAATCTG AAGTTCTACA ATTTCCCCAA GGACATCAAG 
AGCCTGATCA AGTGGTGCCA GAACGCCCGC CTGCCAGTGC AGGCCAAGGA GCCGCGTCAC 
TTTTGCAGCC GACACTTTGA GGATCGCTGT ATTGGCAAGT TCCGACTGAA GCCGTGGGCC 
GTGCCCACCC TCCATCTGGG AGCGCAGTAC GGCAAGATCC ATGACAATCC CAAGAATTTG 
TATGTGGAGG AGAAGCGCTG CTGCCTGAAC TTTTGTCGCC GCAGCCGCTC CTCGGACTTT 
AACATGTCGC TGTATCGTTT CCCCAGAGAC GAGGTGCTCC TGCGACGTTG GTGCTATAAT 
TTAAGGCTGG ATCCGGGCGT GTATCGTGGC AAGAATCACA AAATATGCAG TGCGCATTTT 
ATCAAGGAAG CTTTGGGTCT AAGAAAGCTG TCGCCAGGTG CCGTTCCCAC ATTGCATTTG 
GGTCACAATG ACACCTTTAA TATCTATGAG AACGAACTGT GGCCACCGCC ATCTCCCACT 
GGACAACATG GCGGCAGTCA CCAGCTTCTC CAGCAACAGC AGACGTCGCA GCAGCTGTCG 
CATCATCATT CGTCCCTGCA GCAGCAGCAT CAGCCCATGC ATAGCAAATC CTATCAACGC 
CATTCGGCGG CCTCCACATC CTCCTCCGCC AGTTCGGCCT CTCATTATGT GGACCCCGAG 
ATGAGTGCCT CGTATTTGAA CCTGTCTGCG GGTGGCTCCT CCGGCGGGAT GAATGCCAGC 
GACTGCATGG ATGTGTGCTG CGTGCCAAGC TGCGAGAGCA AGCGGCACAA CAGCGAGAAC 
ATCACATTCC ACACGATACC GCGCAGGCCA GAGCAGATGC GCAAGTGGTG CCACAATCTG 
AAGATACCCG AGGAGAAGAT GCACAAGGGC ATGAGGATTT GTAGCCTGCA CTTTGAACCA 
TACTGCATTG GCGGCTGCAT GCGTCCGTTC GCCGTGCCCA CACTCCATTT GGGGCACGAC 
GATGAAGACA TTCACCGCAA TCCGGATGTG ATCAAGAAGC TGAACATCCG AGAGACCTGC 
TGTGTGGCCG TGTGCAAACG GAATCGCGAC AGAGACCATG CCAACCTCCA TCGTTTCCCC 
AGCAATGTGG CGCTGCTCAC CAAGTGGTGT GCGAATCTGC AGCGGACAGT ACCCGATGGC 
AGCAAACTCT TCAACGATGC CATCTGCGAG GTGCATTTTG AGGATCGTTG TCTGCGCAAC 
AAGAGACTGG AGAAGTGGGC TGTGCCCACT CTGATCCTCG GCCACGACGA CATTGCCTAT 
AAGCTGCCGA CGCCCGAGCA GGTGGCCGAG TTCTATGCCC GTCCCACGGC CCCCAACAAT 
GGCGAGGAGC AGGGCGAGTG CTGTGTGGAG ACGTGCAAAC GGAACCCGAG TGTGGATGAC 
ATCAAATTGT ATCGTCCGCC GGAGGATACT TCGGTGCTGG CCAAATGGGC GCACAATCTG 
CAAACGGAGG CCACTGCCCT CACGAACATG CGGATATGCA ATCTGCACTT TGAGGCCCAC 
TGCATTGGCA AGCGGATGCG TCCGTGGGCC ATACCCACGC TCAATCTGGC TGGAAACATT 
GAGAATCTGT ACGAGAATCC CGAGCATTCG ATGCTGTACA AGCGAAGGAC GCACATCAAA 
CAGAAGGTTC CGGTGACAAA GCCCACGTGG GTGCCTCGCT GCTGTCTGCC GCACTGCCGC 
AAGGTGCGTG CCCTTCACAA TGTCCAGCTG TATCGCTTCC CCAAGCTGAA TCGCTCGACG 
CTGGCCAAGT GGGCACACAA TCTGCAGGTG CCGCAGGTGG GAAGTGCCCA GCGGCGGGTA 
TGTTCCGCCC ACTTTGAGCC GCATGTTTTG AGTAAAAAGT GCCCGGTGCC GCTGGCGGTG 
CCCACATTGG ACTTGAACTC ACCCGCTGGC CACAAGATCT ACCAGAATCC GGCCAAGCTG 
AAGGCCAACA AGCTGTGCCT GCAGCGGGTA TGCATTGTGG AGAGCTGCAG GAAGACCAGA 
GCCCAGGGCG TGCAGCTCTT CCGTCTGCCT CACAGCCCCA CGCAGCTGAG GAAATGGATG 
CACAATATAA GGACACGCCC AAGGGCGGCC ATGAGGAGCC AGTATCGCGT CTGCTCGCGG 
CACTTTGAGA CGCACTCCTT CAACGGTCGA AGGCTGAGCG CCGGGGCCAT TCCCACTTTG 
GAGTTGGGAC ATGACGACGA TGATATCTTC CCGAATGAAG CGCAGGCCTT TGCCGATGAG 
CACTGCGCTG TGGAGGGCTG TGAATCGTCG AAGGAACAGC CCGAAGTGCG GCTCTTCCGC 
TTCCCCACGG ACGACGACGA CATGCTGTGG AAGTGGTGCA ACAACCTCAA GATGAATCCC 
GTCGACTGCC TCGGTGTGCG GATCTGCAAC AAGCATTTCG ATGCCGATTG CATTGGACCC 
AAACATCTGT ATAAGTGGGC CATACCCACG ATGCTGCTCG GCCACGATGA TTCCCAGATC 
GAGCTCATAC TTAACCCTAA GCCGGAGGAA CGCTACGTGG ATCCCGTGTT CAAGTGCATT 
GTCCCAACCT GCGGGAAGAC TCGCCGCTTC GATGAGGTGC AAATGAACAG CTTCCCCAAG 
GATGCGGATC TGTTTCAGCG CTGGCGCCAC AACCTCCGCC TGGAGCATCT GTGCTTCAAG 
GAGCGCGAGA AATACAAGAT CTGCAATGCG CATTTCGAGG ACATGTGCAT TGGCAAGACG 
CGTCTGAACA TTGGTTCCAT ACCCACTCTG GAGCTGGGCC ACGAGGAAAC GGAGGATCTG 
TTCAAGGTGA ATCCGGAAGA TCTGCAGAGC AATCTGTTTG GGCGTCCCCG TCGGCTGCTA 
AGAGGATTGA ACAATGTGAC CATCAAACAG GAGTTGCCAG AGACCGAGGA GCAGGACATA 
AAGCCCGACA TAAGGACCAA CTTTACACAG GTAAAGATTA AGAAATCTCT GGGGGATATT 
AAGTGCTGTG TGCACACGTG TGGACGCAGT CGTTTGGAGC ATGGGGCACG TCTCTTTCCC 
TTCCCCACGG GCAAGCAACA GCACCTCAAG TGGCGCCACA ATCTGCGCCT GGAGCCCGAC 
GAAGTGGACA AAACCACGCG CGTCTGCAGC GCACACTTCA ACAGGCGCTG CATCGATGGA 
AAGCATCTTA GGGGATGGGC CATGCCCACA CAGCAGTTGG GCCACCAAGA ACAGCCTATA 
TACGAGAATC CCAAGAATAT TCCCGGCTTC TTTACGCCCA CCTGTGCGCT GGGCCACTGC 
CGCAAGCGGC GGAGCATTGA CAATGATTTG CGCACGTATC GTTATCCGCG GAGCGAGGAT 
CTCCTCGAGA AGTGGCGCGC AAATCTCAGA CTATCGCTGG ATCAGTGTCG TGGCAGGATC 
TGTGCGGATC ACTTTGAGCC GCAGGTGAGG GGCAAACTGA AGTTGAAGAC GGGAGCAGTA 
CCCACGCTAA AACTGGGCCA TGAGGAGGCT TTGATGTACG ACAATGAGGC TATAAAGGCT 
GGAGTGGCCG AAGAGGAGGC TGGCAGTCCG CCGGCATCGC CTCTGGTGAC ACCCAAAACG 
GAAGTTCTGG ACGAAGAGGA GCGCGAGGAT GATGAGGAGG AGGAGGAGAA CCCCGAAGAA 
GAGCAGCAGG AAAACCATGA TGAGGAGAAG GATGAACACG AGGATGACAC GCCCGAGGGA 
GCAGAGCAGC TGGGCGATGA GGATGACGAC GAGGATCCAG GCAACTATTT TGATCCGTTG 
GAGCTGGTGG AGACGTATGC AGAGCATCCC AGCGACGATG ACAACAGCCA CGAGGAAGCA 
GACGATGCCA GAGAGGAGGA TGAGGAGGAA GAGGAGGAGG CAGAAACTCT CTTGCCTGAT 
ACACCACCCA ACGTAGCAGC AGCAGCAGTC CTTCGCGTGC CGAAACCATG GGAAAGACCT 
CTCGCAGTAG TGCCTCGCCG AGAGAAGCGT CCGAATAACG TGGATCCCAT CTGCTGCCTC 
AAACACTGCC GCAAGGAACG CTCCGCCATG TATCTGCTGA GCACATTTGG CTTTCCAAAG 
GACCAGCAGC TGCTGCTCAA GTGGTGCGCC AACCTCCAAA TGGATCCCTC GAGCTGCATT 
GGTCGCGTCT GCATCGAACA CTTCCAGTCC GAGGTTCTGG GCACGCGAAA ACTCAAACAG 
AATGCGGTGC CCACCCTCAA TGTGGGTCAC GATGTGCCAC TGCGCTACAG CTGCAACGGC 
CAAGAGAGGC CTCAGGCAGC AGCGGCGGCG GCCGCCACGA ACAGCTTCCC CGACGAAATG 
CCACAACATT CGGTTTTTCG GCTTTGGAGC CTGAAACACT GCCGCAAGAG GAAAGTGTCG 
GAGAGTCCAG TTCCAGCAGC AATCAAGACG GAGGAGCAGA TGCAGATGGA GATGGAGGTG 
GAGACTAAGC CAAAGATATG CTGCCTCTCC AGTTGTGGCA ATGTGGAGGG CTACGGCCCG 
GGCGGGTACT TTCAGCCGCT GCCCCAGGAC CAAAGGATAC TGAAAAAATG GCAGCACAAC 
CTGAAGCTAC CATCTGTCAA TCCCGATTCG GATCTTCGTG ACTTTCGCCT GTGCATGGAG 
CACTTTGAGC CGCATCAAAT CGAGAACGGA GCCCCAGTGA AGATGGCCGT TCCGACCCTC 
AAGCTTGGGC ACTCCAGTCC GAATATCTTT AAGAACAGCG AGAGCACGCT GCCGGGATGC 
CTGTGGCCCT CGTGTCCGCC CAATCGCAAG ATCTGCTACG ATCTGCCCGA CAATGAGGCC 
GTTCGAGCGG CCTGGCTGTC GTATGTGCGG CTGCCGCCGG ACAGCCAGGG TCGTCTGTGT 
GGTCTGCACT TTCTGCAGCT GTACGAGGAG GTGGATCTGC CAGGAGATGT ACCCGAAGCG 
GTGCTCGAGC GACTGCAGGG TATCTATGAT CAGGCCTCCA TCTCGCTGAA GTTTCAGTGC 
TCGGTGCAGG GATGTGGCTC CAAGTACAAG CAGGATACGC ATTTGACAAA GCTGCCACGG 
GACGCGGAAC TGCTGGCCAA GTGGCTGCAC AACACCAGGA TCTCCTACGA TCGCTCCTTG 
CATTTTAGCT ACCGCATTTG TCTATTGCAC TTTGAGGCGT TCTGCTTGAA TGGCGTGCGC 
CCGCAGACCT GGGCCATACC TACGCTCCAG CTAAATCACG ACGGAGAGAT CTACCAAAAT 
ACCGCCAAGC AGGAGATCCC AGAGATGCCC CTGAAGCAGG AGATCCTCGA GAATCCCGTG 
AAGCAGGAGA AATCGCACTG TGGGAGAATC TCCAGCCTGA GTCTCTCCAT CCCCCTGCAC 
ATCAAGACGG AACAGGGTCC TGTCCTGCGA CCTCGAGGCA CTTGGGGCAC ATCTTCTCAG 
AGCAGTCCCT GCCTGAGCGC CAGCTCCAGT CCCCGCCTGA AAAACAGAAT CTGCTGCGTC 
TCCGATTGCG GAGAGTACGC CAAATCCCAG CGGCTGTACC GCTTTCCCAC CGCCGAACCG 
GCACTGCTCA AGTGGCTGGT TAATACCCAG CAAAAACCGG GACTCGTGGA CATCCAGAAC 
CTGTTTGTGT GCCAGCTTCA TTTCGAGGCG GACGCCATTA ACCAAACGCA GCTCAGGAGC 
TGGGCCGTGC CCACACTGAG GCTGGGCCAC GATGGGCATG TCATACCAAA TGCCAGACAC 
AATGGGAACA TAGCCAACAG CCAGGAAACG GAGCAGGCCA TGGAGTTCAT TCGGGCCAAC 
TACTGCGCCG TGTTGAGCTG CTTCCAGCCG AAAGGAGATG GTGTACGCTT CTACAAGTAT 
CCCAGCGACA TTGCCATGGC GCGCAGGTGG GCCACGAATC TCAAGCATCG CTCCATGCAG 
GCCAGCAGTC ATGGCTTCCT GGTTTGCCAG TCCCACTTTG CAGCCGACTG TTTTGATCCG 
GAGACGGGAG ACCTGCGCGA GGACGCCGTA CCCGTGGCCA CACTCGCAGA GAACGTGAAA 
TCAGAGGGCC TGCTGCTCCG TTGTCTGGTA AGGGGTTGCT CTACGGATAA CTCTGAAAAA 
GGACTGCTGT TCAAGGTGCC AAAAAAGAAT CGTGTACGGG ATGTGTGGGC CCACAATCTG 
TGGATGCATC CGATAGAGCT GATGGGCGAG CACTACATCT GCGATCGACA TTTCGAGGCG 
CATTGCGTGA ATGAACACAA ATTACTCCAC GCGGGCTCAG TGCCAACCCT CCACCTGGGA 
CACAACGAAC CGCTGGAACT ACTGCCCAAT CCCCAGACCT TCCAGGACTT CCCTGAGGAG 
TGCGAGTGCT GTGTGCCCGG CTGTGGACGC ACCAATCGGA AGGAGGAGGA TCTGCAGTTT 
AGCAAATTTC CCAAGTGGCG AGTCCTGTAT GAAAAGTGGC TGCACAACTT CCGCCTCGAA 
GTGCCCAAGG AGCAGCGCAT CGGGACGCTG AGAGTGTGTC ACATGCACTT TGAGGAGAGC 
TGCTACGATG GCCAGAACGT GCGCAGGGGA GCTATGCCCA CCCTAGAGCT GGGACACTCG 
CATCCAGACA TTTATCGCTC CGACAAGGGA TCGCTGTGGA AGAAGGTTCA CAAGAGACTC 
ACCGATTGTT GCTATCCCGA TTGCTACGAG GAGTGTCACA AGGCCAACAC GAATCGCACG 
ACCTACGATC TGCCCAGCGA TGGGCCACTG CGCGAGTCCT GGCAGCAGCA CATGGCCATC 
CCTGCCAGCG GTGAGGATGG CTCCTCAGTG CTAAAGCTCT GTGCCCTACA CTACATCATT 
ATGTACGAGC ACAGCGAACA GAGCTTCCCA GAACACGGAC CGAATCTACT GCTGGACAAG 
AACTACGAGC ACGCCCGTCA GTTGGCGTAT CTGCGGCGCT TCTTGTGTGC GGTACAGGGC 
TGTCGTTATT TGCAGCCGCG GGATGGGGGT CCGATGCACG GAATACCCCG GCGGAGGGAG 
ATCCTTCGGA TGTGGGTGGA GAATGCCCAG CTGCGGCTGA ACGAGCACGA AATTTACATG 
ACGAAGCTGT GCAGCAAACA TTTTGAGGCC CACTGCCTGT TCGAGGGTAA AAAATGCTAT 
CCCTGGAGCG TGCCAACGCT CCATCTGCCA GAGGCGCAGC CCGGGCAGGT GCTCCACCAG 
AATCCCACCA AGGAGGAGTG GCAGGAAATG AAACAGAGAA TGAAATTGGA AGAGCAGACG 
CCGAAGACGG AAGAGCAGGC AGATGGACTA CTAATGGAAC CCTATGTGAA GATGGAACCC 
CACGACGACG AGTCACAAAC GGAGTCGGAA TTGCTGATAA ATGAGAGCAC ACTGGACTCT 
CAAGAACTCT CTCAAGACTT TCCAACACAA GATCCAATCG AAATGCCCGC CCTGGAGGTG 
CTCCTAGAGG TGAGCCATGT CGAGAAGCTG GATAGCTACG AGAGGAATGA ATACCCTGCG 
GATACCTCTG CCAACACAAA TTCTCCGAAC AAACGTTTCC GCCATCAGTA CAGTGCCCAC 
AAGTGTAGTG TCGAGGGATG TCGCGTGTCG CTCGAGGACC TTGATGGGAA TCTGAAGCTG 
CACAAGCTAC CCAGCTCCAC GGAGGCGGCC GGGAAGTGGC TGTACAACAT TCACGTGGAG 
ATAGAGGATA AATGGCGGAT ACGCGTCTGC AGCCATCACT TCGACAGGCA GTGCCTCAAT 
GGTTCAAGGC TCAGGAGGGG ATCGATGCCC ACTCTGCTGC TGGGGCCGCG TGTTCCAGAG 
ACTATCCATC AGAATGAGTT TGCGCAGCTA CAATTGGACG ATGCACCAGC CCATAATGGC 
AATACATTGG AGCGATCCAT TGGAAAGGTT GCGCAGATAT GCGTTCCACG TCCGTCGCCG 
CCGCGTAAGT CGAGCAAATT CTGCCAGATC GAGGGATGTT CGAACCATCT GACCAGCGAG 
AATATGACGC TACACAAGTT CCCGCACTCG TCGTGGATCT GCACCAAATG GCAGCACAAC 
ACACAGGTGC CGTTCGATCC GGAGTACCGC TGGCGCTATC GCATCTGCAG CGCCCACTTC 
CATCCCGTGT GTATGGTCAA TATGCGGCTG CTGCATGGCA GTGTGCCCAC CCTGAAGCTT 
GGTCCACGGG CACCCGGCGA ACTCTTTGAC AGCGACTTTG AGGCCATAAA CATAAAGATT 
GAAAAAATGG AGAAGATGGA GAGGAAATCT GAGGCTCTGA GAAGCACCAC TGGAGATAGA 
TATCCCACCA TGCAGGCCAT GGGGGAGAAG AAGTTCAAGA CTGAGGAGCT GGAAGATGGA 
ATGGAGGAGG AGGATGACAT GCTCTGCCTG GAGCCAGAGA TGCAGCTATA CGAAGATCAG 
GAAGAACAGC AACAGAAGCC AAAGATAAAT CTTGGAGTCC CCAATGGCGG CTGGAAAACG 
GAACTCCGTT TGCCATCGAA GGGTAGGGTG GCGTTCAATC CGGTGAGATC TGGCTACGAC 
AAGTGCTCGC TGATGCATTG CCAGCGCCAG AGATCGAAGC ACGGCGTCCA CATCTACAAG 
TTCCCCCGAT CGCAGGAGCA CCAACAGCGA TGGATGCACA ATCTGCGCAT ACGCTACGAT 
GAGAAGCGAC CCTGGAAGTT TATGGTCTGC AGCGTGCACT TTGAACCGCA TTGCATACGG 
CTGCGGAAGC TGCGGCCCTG GGCAGTTCCC ACGCTAGAGC TGGGAGACAA TGTGCCCGAG 
GACATCTATA CGAACGAGCA GTGCCAGATG TTTGCCAGTG GACAGGGAGG AGAGATCAAT 
GGCATCGATA GCGATGAGGT GGAGGCGGAG CCGGAGGCAG AAGGGGAGAG CGATGGGAAT 
GATGAGGATG GCCTGCAGGA GGACGAGGAT GAGGAGACAG ACGACCAGGA GCCCATCGCC 
AAGAAGCGTC GTCGCTCGCG GCTGGATGCC GTCTGGCCTC CCGGCCAGGC GCCACCATGG 
AAGGTGAAAC AATGCTGTCT TCCCTACTGT CGCAGTCCAC GCGGCGAGGG CATCAAGCTG 
TTTCGACTGC CCAACAAAGT CAACTCCATC CGCAACTGGG AGCTGGCCAC GGGCATGAAG 
TTCAAGGAGT CGCAGCGCAA CACGAGACTC ATCTGCAGCC GCCACTTTGA TCCGGAGCTG 
ATCGGAGTGC GTCGTCTGAT GCGCAATGCC ATTCCCACCA GGCATCTGGG ACCCACGGGC 
GATGTAAAGC CAGTGATGGC TCCACCGACA GCTGGTCCCA AATGCTGTAT GGCAGATTGT 
ACCTATGATG TGGCGGATGT GAAGCTGCAC AAGTTTCCCA GCAATCCCAA ACTCCTGAAG 
GAGTGGTGCC AGGCATTAAG GGTCACGGAT ATGCAAAGGT ATCGCGGCAA GCACATTTGC 
TCCGCCCATC TACCTGTCCA CGAGGCCGTA CAGTGCATTG TTTGTGGCGC GGAGAAAGCA 
CCCCTGCTGC CGATGCTTAA TTTTCCCGCT AACCGGAATC AGCGCGCCAA ATGGTGCTAC 
AATCTTAAGA TCGAAACGAT ACCCAAGTGG GACATATCCA AGCACATTTG CTGTAAACAC 
TTTGAGCCAT ATTGTTTTGC AGAGGCGGGT CTCCTAAAGC CAGAGGCGGC ACCCACGCTG 
CATTTGAATC ACAATGATAC AAATATATTC CTTAACGATT GTGCCATAAA TCCTGCCTAC 
AGCGTATGCG TGAAGGATGA ACCCATGGAC AATCAGGTCC TGTCATTGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355744.3
CDS	425..12058
Translation	MSQHNPHSHPHYHQTQHHNHQQLQQQQQQQQPHSNWYSHVASYPPPPPQYHASATSAFAATSTPLSNDIYRNFGRTDNNDSPSFGHRNSSNTNFGDNNGRYFNDAPQDYYVANFIDNYRSGPRSQNFGDQRSSYMGHDIMSGTYYPYIKSEPMESAYQQPQNPMAPPPAPDMIIKSEPMDEHPYKSNYIDDNTPFADFNKFNEFSGDMLSPKVELTVKDETYGKTSSSNSSSSFARRKAQQQQTTDRSAESLPICQRCKEVFFKKQSYLRHVAESSCGIQEYDFKCNICPMSFMTTEELQRHKQLHRADKFFCHKYCGKHFDTIAECESHEYMQHEYERFVCNMCSGTFATREQLYAHLPQHKFQQRYDCPICRLWYQTAVELHEHRLSAPYFCGKYYTSQQQQLATSQGNYKLQDCHMATMEIPTAPLHKATPSNPSALPATAALSSLLQQRQANADGTAAMFAAASSSSASLKSEVSVKLERSYSNSTSESSYSHQDNSSYNNAYGSDNSIHGGALAGPQAHSSTLDDSEDALCCVPLCGVRKSTSPTLQFFTFPKDEKYLNQWLHNLKMFHIPAASYATFRICSMHFPKRCINRYSLCYWAVPTFNLGHDDVANLYQNRELTNTFTTGEVARCSMPHCTSQRGESNLKFYNFPKDIKSLIKWCQNARLPVQAKEPRHFCSRHFEDRCIGKFRLKPWAVPTLHLGAQYGKIHDNPKNLYVEEKRCCLNFCRRSRSSDFNMSLYRFPRDEVLLRRWCYNLRLDPGVYRGKNHKICSAHFIKEALGLRKLSPGAVPTLHLGHNDTFNIYENELWPPPSPTGQHGGSHQLLQQQQTSQQLSHHHSSLQQQHQPMHSKSYQRHSAASTSSSASSASHYVDPEMSASYLNLSAGGSSGGMNASDCMDVCCVPSCESKRHNSENITFHTIPRRPEQMRKWCHNLKIPEEKMHKGMRICSLHFEPYCIGGCMRPFAVPTLHLGHDDEDIHRNPDVIKKLNIRETCCVAVCKRNRDRDHANLHRFPSNVALLTKWCANLQRTVPDGSKLFNDAICEVHFEDRCLRNKRLEKWAVPTLILGHDDIAYKLPTPEQVAEFYARPTAPNNGEEQGECCVETCKRNPSVDDIKLYRPPEDTSVLAKWAHNLQTEATALTNMRICNLHFEAHCIGKRMRPWAIPTLNLAGNIENLYENPEHSMLYKRRTHIKQKVPVTKPTWVPRCCLPHCRKVRALHNVQLYRFPKLNRSTLAKWAHNLQVPQVGSAQRRVCSAHFEPHVLSKKCPVPLAVPTLDLNSPAGHKIYQNPAKLKANKLCLQRVCIVESCRKTRAQGVQLFRLPHSPTQLRKWMHNIRTRPRAAMRSQYRVCSRHFETHSFNGRRLSAGAIPTLELGHDDDDIFPNEAQAFADEHCAVEGCESSKEQPEVRLFRFPTDDDDMLWKWCNNLKMNPVDCLGVRICNKHFDADCIGPKHLYKWAIPTMLLGHDDSQIELILNPKPEERYVDPVFKCIVPTCGKTRRFDEVQMNSFPKDADLFQRWRHNLRLEHLCFKEREKYKICNAHFEDMCIGKTRLNIGSIPTLELGHEETEDLFKVNPEDLQSNLFGRPRRLLRGLNNVTIKQELPETEEQDIKPDIRTNFTQVKIKKSLGDIKCCVHTCGRSRLEHGARLFPFPTGKQQHLKWRHNLRLEPDEVDKTTRVCSAHFNRRCIDGKHLRGWAMPTQQLGHQEQPIYENPKNIPGFFTPTCALGHCRKRRSIDNDLRTYRYPRSEDLLEKWRANLRLSLDQCRGRICADHFEPQVRGKLKLKTGAVPTLKLGHEEALMYDNEAIKAGVAEEEAGSPPASPLVTPKTEVLDEEEREDDEEEEENPEEEQQENHDEEKDEHEDDTPEGAEQLGDEDDDEDPGNYFDPLELVETYAEHPSDDDNSHEEADDAREEDEEEEEEAETLLPDTPPNVAAAAVLRVPKPWERPLAVVPRREKRPNNVDPICCLKHCRKERSAMYLLSTFGFPKDQQLLLKWCANLQMDPSSCIGRVCIEHFQSEVLGTRKLKQNAVPTLNVGHDVPLRYSCNGQERPQAAAAAAATNSFPDEMPQHSVFRLWSLKHCRKRKVSESPVPAAIKTEEQMQMEMEVETKPKICCLSSCGNVEGYGPGGYFQPLPQDQRILKKWQHNLKLPSVNPDSDLRDFRLCMEHFEPHQIENGAPVKMAVPTLKLGHSSPNIFKNSESTLPGCLWPSCPPNRKICYDLPDNEAVRAAWLSYVRLPPDSQGRLCGLHFLQLYEEVDLPGDVPEAVLERLQGIYDQASISLKFQCSVQGCGSKYKQDTHLTKLPRDAELLAKWLHNTRISYDRSLHFSYRICLLHFEAFCLNGVRPQTWAIPTLQLNHDGEIYQNTAKQEIPEMPLKQEILENPVKQEKSHCGRISSLSLSIPLHIKTEQGPVLRPRGTWGTSSQSSPCLSASSSPRLKNRICCVSDCGEYAKSQRLYRFPTAEPALLKWLVNTQQKPGLVDIQNLFVCQLHFEADAINQTQLRSWAVPTLRLGHDGHVIPNARHNGNIANSQETEQAMEFIRANYCAVLSCFQPKGDGVRFYKYPSDIAMARRWATNLKHRSMQASSHGFLVCQSHFAADCFDPETGDLREDAVPVATLAENVKSEGLLLRCLVRGCSTDNSEKGLLFKVPKKNRVRDVWAHNLWMHPIELMGEHYICDRHFEAHCVNEHKLLHAGSVPTLHLGHNEPLELLPNPQTFQDFPEECECCVPGCGRTNRKEEDLQFSKFPKWRVLYEKWLHNFRLEVPKEQRIGTLRVCHMHFEESCYDGQNVRRGAMPTLELGHSHPDIYRSDKGSLWKKVHKRLTDCCYPDCYEECHKANTNRTTYDLPSDGPLRESWQQHMAIPASGEDGSSVLKLCALHYIIMYEHSEQSFPEHGPNLLLDKNYEHARQLAYLRRFLCAVQGCRYLQPRDGGPMHGIPRRREILRMWVENAQLRLNEHEIYMTKLCSKHFEAHCLFEGKKCYPWSVPTLHLPEAQPGQVLHQNPTKEEWQEMKQRMKLEEQTPKTEEQADGLLMEPYVKMEPHDDESQTESELLINESTLDSQELSQDFPTQDPIEMPALEVLLEVSHVEKLDSYERNEYPADTSANTNSPNKRFRHQYSAHKCSVEGCRVSLEDLDGNLKLHKLPSSTEAAGKWLYNIHVEIEDKWRIRVCSHHFDRQCLNGSRLRRGSMPTLLLGPRVPETIHQNEFAQLQLDDAPAHNGNTLERSIGKVAQICVPRPSPPRKSSKFCQIEGCSNHLTSENMTLHKFPHSSWICTKWQHNTQVPFDPEYRWRYRICSAHFHPVCMVNMRLLHGSVPTLKLGPRAPGELFDSDFEAINIKIEKMEKMERKSEALRSTTGDRYPTMQAMGEKKFKTEELEDGMEEEDDMLCLEPEMQLYEDQEEQQQKPKINLGVPNGGWKTELRLPSKGRVAFNPVRSGYDKCSLMHCQRQRSKHGVHIYKFPRSQEHQQRWMHNLRIRYDEKRPWKFMVCSVHFEPHCIRLRKLRPWAVPTLELGDNVPEDIYTNEQCQMFASGQGGEINGIDSDEVEAEPEAEGESDGNDEDGLQEDEDEETDDQEPIAKKRRRSRLDAVWPPGQAPPWKVKQCCLPYCRSPRGEGIKLFRLPNKVNSIRNWELATGMKFKESQRNTRLICSRHFDPELIGVRRLMRNAIPTRHLGPTGDVKPVMAPPTAGPKCCMADCTYDVADVKLHKFPSNPKLLKEWCQALRVTDMQRYRGKHICSAHLPVHEAVQCIVCGAEKAPLLPMLNFPANRNQRAKWCYNLKIETIPKWDISKHICCKHFEPYCFAEAGLLKPEAAPTLHLNHNDTNIFLNDCAINPAYSVCVKDEPMDNQVLSLV

Target ORF information:

RefSeq Version	XM_001355708.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10453), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355708.3

ORF Insert Sequence:

ATGTCACAGC ATAATCCACA TTCCCATCCG CACTACCATC AGACACAACA CCACAACCAT 
CAGCAGTTGC AGCAGCAGCA ACAACAACAG CAGCCACACA GCAATTGGTA CTCACATGTT 
GCTTCCTACC CCCCACCGCC ACCCCAGTAC CATGCGTCGG CTACCTCCGC ATTTGCCGCT 
ACCTCCACAC CGTTATCGAA CGATATATAT AGGAACTTTG GTCGTACCGA CAACAATGAT 
TCCCCCAGCT TTGGCCACCG GAATTCGTCA AACACCAACT TCGGCGACAA CAATGGGCGT 
TACTTCAATG ATGCTCCACA AGATTATTAT GTGGCCAACT TTATTGACAA TTATCGCAGT 
GGCCCGAGGT CGCAAAACTT TGGGGATCAG CGTTCCTCCT ATATGGGACA TGATATAATG 
TCCGGAACTT ATTATCCGTA CATAAAGAGC GAACCCATGG AGTCAGCGTA TCAGCAGCCA 
CAGAATCCGA TGGCCCCGCC CCCAGCGCCA GATATGATAA TAAAATCGGA ACCCATGGAT 
GAACATCCCT ACAAGTCAAA CTATATTGAT GACAATACGC CCTTTGCTGA TTTTAACAAG 
TTCAACGAAT TCAGCGGCGA TATGCTAAGC CCCAAAGTCG AGCTAACCGT CAAGGATGAG 
ACTTACGGAA AGACTTCCAG CAGCAATAGC AGCAGCAGCT TTGCACGTCG AAAAGCCCAG 
CAACAGCAAA CGACAGATCG TTCGGCGGAG AGTCTGCCCA TCTGCCAGCG CTGCAAGGAG 
GTCTTCTTCA AAAAGCAATC CTATCTGAGG CATGTGGCCG AGAGCAGTTG TGGCATTCAG 
GAGTACGATT TCAAGTGCAA CATATGCCCC ATGTCCTTCA TGACCACTGA AGAGCTGCAG 
CGCCACAAGC AACTGCATCG TGCAGACAAG TTCTTCTGCC ACAAATACTG CGGCAAGCAT 
TTCGATACGA TAGCCGAGTG CGAATCGCAC GAGTACATGC AGCACGAGTA TGAGAGATTT 
GTTTGTAATA TGTGCTCTGG AACCTTTGCC ACGCGGGAAC AGCTGTATGC CCACTTGCCG 
CAACACAAGT TTCAGCAGCG TTACGACTGT CCCATCTGCC GTTTGTGGTA TCAAACAGCC 
GTCGAATTGC ATGAGCATCG ACTGTCGGCT CCATACTTTT GCGGCAAGTA CTATACCAGT 
CAACAGCAGC AGCTTGCTAC GAGCCAGGGG AATTACAAGC TGCAGGACTG CCATATGGCC 
ACCATGGAAA TACCCACTGC ACCACTGCAC AAGGCAACGC CTTCCAATCC CTCAGCCTTG 
CCAGCCACAG CTGCCTTGAG CTCTCTGTTG CAACAGCGCC AGGCAAATGC CGATGGGACT 
GCGGCCATGT TTGCTGCGGC CTCCTCTTCC TCCGCCTCGC TGAAGAGCGA GGTGAGCGTG 
AAGCTAGAGC GTAGCTACAG CAACTCCACC AGCGAGTCCT CTTACAGCCA TCAGGACAAC 
AGCAGCTACA ACAATGCCTA TGGCAGCGAC AACTCCATCC ATGGCGGAGC ACTGGCCGGA 
CCACAGGCTC ACTCCTCAAC GCTGGACGAC TCCGAGGATG CCCTGTGTTG TGTGCCCCTG 
TGCGGCGTCA GAAAAAGCAC CAGTCCAACG CTGCAGTTCT TTACGTTTCC AAAGGATGAA 
AAGTATCTGA ATCAGTGGCT ACACAACCTC AAGATGTTCC ACATACCAGC GGCGAGCTAT 
GCGACATTTC GCATCTGTAG CATGCACTTC CCGAAGCGTT GTATCAATCG GTATTCGCTG 
TGCTATTGGG CGGTGCCCAC CTTCAATCTG GGGCACGACG ATGTGGCCAA TCTGTACCAG 
AACCGTGAGT TAACCAACAC TTTTACCACT GGAGAGGTGG CACGCTGCAG CATGCCGCAT 
TGCACCAGCC AGCGGGGGGA GAGCAATCTG AAGTTCTACA ATTTCCCCAA GGACATCAAG 
AGCCTGATCA AGTGGTGCCA GAACGCCCGC CTGCCAGTGC AGGCCAAGGA GCCGCGTCAC 
TTTTGCAGCC GACACTTTGA GGATCGCTGT ATTGGCAAGT TCCGACTGAA GCCGTGGGCC 
GTGCCCACCC TCCATCTGGG AGCGCAGTAC GGCAAGATCC ATGACAATCC CAAGAATTTG 
TATGTGGAGG AGAAGCGCTG CTGCCTGAAC TTTTGTCGCC GCAGCCGCTC CTCGGACTTT 
AACATGTCGC TGTATCGTTT CCCCAGAGAC GAGGTGCTCC TGCGACGTTG GTGCTATAAT 
TTAAGGCTGG ATCCGGGCGT GTATCGTGGC AAGAATCACA AAATATGCAG TGCGCATTTT 
ATCAAGGAAG CTTTGGGTCT AAGAAAGCTG TCGCCAGGTG CCGTTCCCAC ATTGCATTTG 
GGTCACAATG ACACCTTTAA TATCTATGAG AACGAACTGT GGCCACCGCC ATCTCCCACT 
GGACAACATG GCGGCAGTCA CCAGCTTCTC CAGCAACAGC AGACGTCGCA GCAGCTGTCG 
CATCATCATT CGTCCCTGCA GCAGCAGCAT CAGCCCATGC ATAGCAAATC CTATCAACGC 
CATTCGGCGG CCTCCACATC CTCCTCCGCC AGTTCGGCCT CTCATTATGT GGACCCCGAG 
ATGAGTGCCT CGTATTTGAA CCTGTCTGCG GGTGGCTCCT CCGGCGGGAT GAATGCCAGC 
GACTGCATGG ATGTGTGCTG CGTGCCAAGC TGCGAGAGCA AGCGGCACAA CAGCGAGAAC 
ATCACATTCC ACACGATACC GCGCAGGCCA GAGCAGATGC GCAAGTGGTG CCACAATCTG 
AAGATACCCG AGGAGAAGAT GCACAAGGGC ATGAGGATTT GTAGCCTGCA CTTTGAACCA 
TACTGCATTG GCGGCTGCAT GCGTCCGTTC GCCGTGCCCA CACTCCATTT GGGGCACGAC 
GATGAAGACA TTCACCGCAA TCCGGATGTG ATCAAGAAGC TGAACATCCG AGAGACCTGC 
TGTGTGGCCG TGTGCAAACG GAATCGCGAC AGAGACCATG CCAACCTCCA TCGTTTCCCC 
AGCAATGTGG CGCTGCTCAC CAAGTGGTGT GCGAATCTGC AGCGGACAGT ACCCGATGGC 
AGCAAACTCT TCAACGATGC CATCTGCGAG GTGCATTTTG AGGATCGTTG TCTGCGCAAC 
AAGAGACTGG AGAAGTGGGC TGTGCCCACT CTGATCCTCG GCCACGACGA CATTGCCTAT 
AAGCTGCCGA CGCCCGAGCA GGTGGCCGAG TTCTATGCCC GTCCCACGGC CCCCAACAAT 
GGCGAGGAGC AGGGCGAGTG CTGTGTGGAG ACGTGCAAAC GGAACCCGAG TGTGGATGAC 
ATCAAATTGT ATCGTCCGCC GGAGGATACT TCGGTGCTGG CCAAATGGGC GCACAATCTG 
CAAACGGAGG CCACTGCCCT CACGAACATG CGGATATGCA ATCTGCACTT TGAGGCCCAC 
TGCATTGGCA AGCGGATGCG TCCGTGGGCC ATACCCACGC TCAATCTGGC TGGAAACATT 
GAGAATCTGT ACGAGAATCC CGAGCATTCG ATGCTGTACA AGCGAAGGAC GCACATCAAA 
CAGAAGGTTC CGGTGACAAA GCCCACGTGG GTGCCTCGCT GCTGTCTGCC GCACTGCCGC 
AAGGTGCGTG CCCTTCACAA TGTCCAGCTG TATCGCTTCC CCAAGCTGAA TCGCTCGACG 
CTGGCCAAGT GGGCACACAA TCTGCAGGTG CCGCAGGTGG GAAGTGCCCA GCGGCGGGTA 
TGTTCCGCCC ACTTTGAGCC GCATGTTTTG AGTAAAAAGT GCCCGGTGCC GCTGGCGGTG 
CCCACATTGG ACTTGAACTC ACCCGCTGGC CACAAGATCT ACCAGAATCC GGCCAAGCTG 
AAGGCCAACA AGCTGTGCCT GCAGCGGGTA TGCATTGTGG AGAGCTGCAG GAAGACCAGA 
GCCCAGGGCG TGCAGCTCTT CCGTCTGCCT CACAGCCCCA CGCAGCTGAG GAAATGGATG 
CACAATATAA GGACACGCCC AAGGGCGGCC ATGAGGAGCC AGTATCGCGT CTGCTCGCGG 
CACTTTGAGA CGCACTCCTT CAACGGTCGA AGGCTGAGCG CCGGGGCCAT TCCCACTTTG 
GAGTTGGGAC ATGACGACGA TGATATCTTC CCGAATGAAG CGCAGGCCTT TGCCGATGAG 
CACTGCGCTG TGGAGGGCTG TGAATCGTCG AAGGAACAGC CCGAAGTGCG GCTCTTCCGC 
TTCCCCACGG ACGACGACGA CATGCTGTGG AAGTGGTGCA ACAACCTCAA GATGAATCCC 
GTCGACTGCC TCGGTGTGCG GATCTGCAAC AAGCATTTCG ATGCCGATTG CATTGGACCC 
AAACATCTGT ATAAGTGGGC CATACCCACG ATGCTGCTCG GCCACGATGA TTCCCAGATC 
GAGCTCATAC TTAACCCTAA GCCGGAGGAA CGCTACGTGG ATCCCGTGTT CAAGTGCATT 
GTCCCAACCT GCGGGAAGAC TCGCCGCTTC GATGAGGTGC AAATGAACAG CTTCCCCAAG 
GATGCGGATC TGTTTCAGCG CTGGCGCCAC AACCTCCGCC TGGAGCATCT GTGCTTCAAG 
GAGCGCGAGA AATACAAGAT CTGCAATGCG CATTTCGAGG ACATGTGCAT TGGCAAGACG 
CGTCTGAACA TTGGTTCCAT ACCCACTCTG GAGCTGGGCC ACGAGGAAAC GGAGGATCTG 
TTCAAGGTGA ATCCGGAAGA TCTGCAGAGC AATCTGTTTG GGCGTCCCCG TCGGCTGCTA 
AGAGGATTGA ACAATGTGAC CATCAAACAG GAGTTGCCAG AGACCGAGGA GCAGGACATA 
AAGCCCGACA TAAGGACCAA CTTTACACAG GTAAAGATTA AGAAATCTCT GGGGGATATT 
AAGTGCTGTG TGCACACGTG TGGACGCAGT CGTTTGGAGC ATGGGGCACG TCTCTTTCCC 
TTCCCCACGG GCAAGCAACA GCACCTCAAG TGGCGCCACA ATCTGCGCCT GGAGCCCGAC 
GAAGTGGACA AAACCACGCG CGTCTGCAGC GCACACTTCA ACAGGCGCTG CATCGATGGA 
AAGCATCTTA GGGGATGGGC CATGCCCACA CAGCAGTTGG GCCACCAAGA ACAGCCTATA 
TACGAGAATC CCAAGAATAT TCCCGGCTTC TTTACGCCCA CCTGTGCGCT GGGCCACTGC 
CGCAAGCGGC GGAGCATTGA CAATGATTTG CGCACGTATC GTTATCCGCG GAGCGAGGAT 
CTCCTCGAGA AGTGGCGCGC AAATCTCAGA CTATCGCTGG ATCAGTGTCG TGGCAGGATC 
TGTGCGGATC ACTTTGAGCC GCAGGTGAGG GGCAAACTGA AGTTGAAGAC GGGAGCAGTA 
CCCACGCTAA AACTGGGCCA TGAGGAGGCT TTGATGTACG ACAATGAGGC TATAAAGGCT 
GGAGTGGCCG AAGAGGAGGC TGGCAGTCCG CCGGCATCGC CTCTGGTGAC ACCCAAAACG 
GAAGTTCTGG ACGAAGAGGA GCGCGAGGAT GATGAGGAGG AGGAGGAGAA CCCCGAAGAA 
GAGCAGCAGG AAAACCATGA TGAGGAGAAG GATGAACACG AGGATGACAC GCCCGAGGGA 
GCAGAGCAGC TGGGCGATGA GGATGACGAC GAGGATCCAG GCAACTATTT TGATCCGTTG 
GAGCTGGTGG AGACGTATGC AGAGCATCCC AGCGACGATG ACAACAGCCA CGAGGAAGCA 
GACGATGCCA GAGAGGAGGA TGAGGAGGAA GAGGAGGAGG CAGAAACTCT CTTGCCTGAT 
ACACCACCCA ACGTAGCAGC AGCAGCAGTC CTTCGCGTGC CGAAACCATG GGAAAGACCT 
CTCGCAGTAG TGCCTCGCCG AGAGAAGCGT CCGAATAACG TGGATCCCAT CTGCTGCCTC 
AAACACTGCC GCAAGGAACG CTCCGCCATG TATCTGCTGA GCACATTTGG CTTTCCAAAG 
GACCAGCAGC TGCTGCTCAA GTGGTGCGCC AACCTCCAAA TGGATCCCTC GAGCTGCATT 
GGTCGCGTCT GCATCGAACA CTTCCAGTCC GAGGTTCTGG GCACGCGAAA ACTCAAACAG 
AATGCGGTGC CCACCCTCAA TGTGGGTCAC GATGTGCCAC TGCGCTACAG CTGCAACGGC 
CAAGAGAGGC CTCAGGCAGC AGCGGCGGCG GCCGCCACGA ACAGCTTCCC CGACGAAATG 
CCACAACATT CGGTTTTTCG GCTTTGGAGC CTGAAACACT GCCGCAAGAG GAAAGTGTCG 
GAGAGTCCAG TTCCAGCAGC AATCAAGACG GAGGAGCAGA TGCAGATGGA GATGGAGGTG 
GAGACTAAGC CAAAGATATG CTGCCTCTCC AGTTGTGGCA ATGTGGAGGG CTACGGCCCG 
GGCGGGTACT TTCAGCCGCT GCCCCAGGAC CAAAGGATAC TGAAAAAATG GCAGCACAAC 
CTGAAGCTAC CATCTGTCAA TCCCGATTCG GATCTTCGTG ACTTTCGCCT GTGCATGGAG 
CACTTTGAGC CGCATCAAAT CGAGAACGGA GCCCCAGTGA AGATGGCCGT TCCGACCCTC 
AAGCTTGGGC ACTCCAGTCC GAATATCTTT AAGAACAGCG AGAGCACGCT GCCGGGATGC 
CTGTGGCCCT CGTGTCCGCC CAATCGCAAG ATCTGCTACG ATCTGCCCGA CAATGAGGCC 
GTTCGAGCGG CCTGGCTGTC GTATGTGCGG CTGCCGCCGG ACAGCCAGGG TCGTCTGTGT 
GGTCTGCACT TTCTGCAGCT GTACGAGGAG GTGGATCTGC CAGGAGATGT ACCCGAAGCG 
GTGCTCGAGC GACTGCAGGG TATCTATGAT CAGGCCTCCA TCTCGCTGAA GTTTCAGTGC 
TCGGTGCAGG GATGTGGCTC CAAGTACAAG CAGGATACGC ATTTGACAAA GCTGCCACGG 
GACGCGGAAC TGCTGGCCAA GTGGCTGCAC AACACCAGGA TCTCCTACGA TCGCTCCTTG 
CATTTTAGCT ACCGCATTTG TCTATTGCAC TTTGAGGCGT TCTGCTTGAA TGGCGTGCGC 
CCGCAGACCT GGGCCATACC TACGCTCCAG CTAAATCACG ACGGAGAGAT CTACCAAAAT 
ACCGCCAAGC AGGAGATCCC AGAGATGCCC CTGAAGCAGG AGATCCTCGA GAATCCCGTG 
AAGCAGGAGA AATCGCACTG TGGGAGAATC TCCAGCCTGA GTCTCTCCAT CCCCCTGCAC 
ATCAAGACGG AACAGGGTCC TGTCCTGCGA CCTCGAGGCA CTTGGGGCAC ATCTTCTCAG 
AGCAGTCCCT GCCTGAGCGC CAGCTCCAGT CCCCGCCTGA AAAACAGAAT CTGCTGCGTC 
TCCGATTGCG GAGAGTACGC CAAATCCCAG CGGCTGTACC GCTTTCCCAC CGCCGAACCG 
GCACTGCTCA AGTGGCTGGT TAATACCCAG CAAAAACCGG GACTCGTGGA CATCCAGAAC 
CTGTTTGTGT GCCAGCTTCA TTTCGAGGCG GACGCCATTA ACCAAACGCA GCTCAGGAGC 
TGGGCCGTGC CCACACTGAG GCTGGGCCAC GATGGGCATG TCATACCAAA TGCCAGACAC 
AATGGGAACA TAGCCAACAG CCAGGAAACG GAGCAGGCCA TGGAGTTCAT TCGGGCCAAC 
TACTGCGCCG TGTTGAGCTG CTTCCAGCCG AAAGGAGATG GTGTACGCTT CTACAAGTAT 
CCCAGCGACA TTGCCATGGC GCGCAGGTGG GCCACGAATC TCAAGCATCG CTCCATGCAG 
GCCAGCAGTC ATGGCTTCCT GGTTTGCCAG TCCCACTTTG CAGCCGACTG TTTTGATCCG 
GAGACGGGAG ACCTGCGCGA GGACGCCGTA CCCGTGGCCA CACTCGCAGA GAACGTGAAA 
TCAGAGGGCC TGCTGCTCCG TTGTCTGGTA AGGGGTTGCT CTACGGATAA CTCTGAAAAA 
GGACTGCTGT TCAAGGTGCC AAAAAAGAAT CGTGTACGGG ATGTGTGGGC CCACAATCTG 
TGGATGCATC CGATAGAGCT GATGGGCGAG CACTACATCT GCGATCGACA TTTCGAGGCG 
CATTGCGTGA ATGAACACAA ATTACTCCAC GCGGGCTCAG TGCCAACCCT CCACCTGGGA 
CACAACGAAC CGCTGGAACT ACTGCCCAAT CCCCAGACCT TCCAGGACTT CCCTGAGGAG 
TGCGAGTGCT GTGTGCCCGG CTGTGGACGC ACCAATCGGA AGGAGGAGGA TCTGCAGTTT 
AGCAAATTTC CCAAGTGGCG AGTCCTGTAT GAAAAGTGGC TGCACAACTT CCGCCTCGAA 
GTGCCCAAGG AGCAGCGCAT CGGGACGCTG AGAGTGTGTC ACATGCACTT TGAGGAGAGC 
TGCTACGATG GCCAGAACGT GCGCAGGGGA GCTATGCCCA CCCTAGAGCT GGGACACTCG 
CATCCAGACA TTTATCGCTC CGACAAGGGA TCGCTGTGGA AGAAGGTTCA CAAGAGACTC 
ACCGATTGTT GCTATCCCGA TTGCTACGAG GAGTGTCACA AGGCCAACAC GAATCGCACG 
ACCTACGATC TGCCCAGCGA TGGGCCACTG CGCGAGTCCT GGCAGCAGCA CATGGCCATC 
CCTGCCAGCG GTGAGGATGG CTCCTCAGTG CTAAAGCTCT GTGCCCTACA CTACATCATT 
ATGTACGAGC ACAGCGAACA GAGCTTCCCA GAACACGGAC CGAATCTACT GCTGGACAAG 
AACTACGAGC ACGCCCGTCA GTTGGCGTAT CTGCGGCGCT TCTTGTGTGC GGTACAGGGC 
TGTCGTTATT TGCAGCCGCG GGATGGGGGT CCGATGCACG GAATACCCCG GCGGAGGGAG 
ATCCTTCGGA TGTGGGTGGA GAATGCCCAG CTGCGGCTGA ACGAGCACGA AATTTACATG 
ACGAAGCTGT GCAGCAAACA TTTTGAGGCC CACTGCCTGT TCGAGGGTAA AAAATGCTAT 
CCCTGGAGCG TGCCAACGCT CCATCTGCCA GAGGCGCAGC CCGGGCAGGT GCTCCACCAG 
AATCCCACCA AGGAGGAGTG GCAGGAAATG AAACAGAGAA TGAAATTGGA AGAGCAGACG 
CCGAAGACGG AAGAGCAGGC AGATGGACTA CTAATGGAAC CCTATGTGAA GATGGAACCC 
CACGACGACG AGTCACAAAC GGAGTCGGAA TTGCTGATAA ATGAGAGCAC ACTGGACTCT 
CAAGAACTCT CTCAAGACTT TCCAACACAA GATCCAATCG AAATGCCCGC CCTGGAGGTG 
CTCCTAGAGG TGAGCCATGT CGAGAAGCTG GATAGCTACG AGAGGAATGA ATACCCTGCG 
GATACCTCTG CCAACACAAA TTCTCCGAAC AAACGTTTCC GCCATCAGTA CAGTGCCCAC 
AAGTGTAGTG TCGAGGGATG TCGCGTGTCG CTCGAGGACC TTGATGGGAA TCTGAAGCTG 
CACAAGCTAC CCAGCTCCAC GGAGGCGGCC GGGAAGTGGC TGTACAACAT TCACGTGGAG 
ATAGAGGATA AATGGCGGAT ACGCGTCTGC AGCCATCACT TCGACAGGCA GTGCCTCAAT 
GGTTCAAGGC TCAGGAGGGG ATCGATGCCC ACTCTGCTGC TGGGGCCGCG TGTTCCAGAG 
ACTATCCATC AGAATGAGTT TGCGCAGCTA CAATTGGACG ATGCACCAGC CCATAATGGC 
AATACATTGG AGCGATCCAT TGGAAAGGTT GCGCAGATAT GCGTTCCACG TCCGTCGCCG 
CCGCGTAAGT CGAGCAAATT CTGCCAGATC GAGGGATGTT CGAACCATCT GACCAGCGAG 
AATATGACGC TACACAAGTT CCCGCACTCG TCGTGGATCT GCACCAAATG GCAGCACAAC 
ACACAGGTGC CGTTCGATCC GGAGTACCGC TGGCGCTATC GCATCTGCAG CGCCCACTTC 
CATCCCGTGT GTATGGTCAA TATGCGGCTG CTGCATGGCA GTGTGCCCAC CCTGAAGCTT 
GGTCCACGGG CACCCGGCGA ACTCTTTGAC AGCGACTTTG AGGCCATAAA CATAAAGATT 
GAAAAAATGG AGAAGATGGA GAGGAAATCT GAGGCTCTGA GAAGCACCAC TGGAGATAGA 
TATCCCACCA TGCAGGCCAT GGGGGAGAAG AAGTTCAAGA CTGAGGAGCT GGAAGATGGA 
ATGGAGGAGG AGGATGACAT GCTCTGCCTG GAGCCAGAGA TGCAGCTATA CGAAGATCAG 
GAAGAACAGC AACAGAAGCC AAAGATAAAT CTTGGAGTCC CCAATGGCGG CTGGAAAACG 
GAACTCCGTT TGCCATCGAA GGGTAGGGTG GCGTTCAATC CGGTGAGATC TGGCTACGAC 
AAGTGCTCGC TGATGCATTG CCAGCGCCAG AGATCGAAGC ACGGCGTCCA CATCTACAAG 
TTCCCCCGAT CGCAGGAGCA CCAACAGCGA TGGATGCACA ATCTGCGCAT ACGCTACGAT 
GAGAAGCGAC CCTGGAAGTT TATGGTCTGC AGCGTGCACT TTGAACCGCA TTGCATACGG 
CTGCGGAAGC TGCGGCCCTG GGCAGTTCCC ACGCTAGAGC TGGGAGACAA TGTGCCCGAG 
GACATCTATA CGAACGAGCA GTGCCAGATG TTTGCCAGTG GACAGGGAGG AGAGATCAAT 
GGCATCGATA GCGATGAGGT GGAGGCGGAG CCGGAGGCAG AAGGGGAGAG CGATGGGAAT 
GATGAGGATG GCCTGCAGGA GGACGAGGAT GAGGAGACAG ACGACCAGGA GCCCATCGCC 
AAGAAGCGTC GTCGCTCGCG GCTGGATGCC GTCTGGCCTC CCGGCCAGGC GCCACCATGG 
AAGGTGAAAC AATGCTGTCT TCCCTACTGT CGCAGTCCAC GCGGCGAGGG CATCAAGCTG 
TTTCGACTGC CCAACAAAGT CAACTCCATC CGCAACTGGG AGCTGGCCAC GGGCATGAAG 
TTCAAGGAGT CGCAGCGCAA CACGAGACTC ATCTGCAGCC GCCACTTTGA TCCGGAGCTG 
ATCGGAGTGC GTCGTCTGAT GCGCAATGCC ATTCCCACCA GGCATCTGGG ACCCACGGGC 
GATGTAAAGC CAGTGATGGC TCCACCGACA GCTGGTCCCA AATGCTGTAT GGCAGATTGT 
ACCTATGATG TGGCGGATGT GAAGCTGCAC AAGTTTCCCA GCAATCCCAA ACTCCTGAAG 
GAGTGGTGCC AGGCATTAAG GGTCACGGAT ATGCAAAGGT ATCGCGGCAA GCACATTTGC 
TCCGCCCATC TACCTGTCCA CGAGGCCGTA CAGTGCATTG TTTGTGGCGC GGAGAAAGCA 
CCCCTGCTGC CGATGCTTAA TTTTCCCGCT AACCGGAATC AGCGCGCCAA ATGGTGCTAC 
AATCTTAAGA TCGAAACGAT ACCCAAGTGG GACATATCCA AGCACATTTG CTGTAAACAC 
TTTGAGCCAT ATTGTTTTGC AGAGGCGGGT CTCCTAAAGC CAGAGGCGGC ACCCACGCTG 
CATTTGAATC ACAATGATAC AAATATATTC CTTAACGATT GTGCCATAAA TCCTGCCTAC 
AGCGTATGCG TGAAGGATGA ACCCATGGAC AATCAGGTCC TGTCATTGGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10453 ( XM_015181414.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036900.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10453), partial mRNA.

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CloneID	ODe18827
Clone ID Related Accession (Same CDS sequence)	XM_015181414.1
Accession Version	XM_015181414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589962). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGAATTGA AATGGGTTGA GGTTGTGTCT ATGTCGGAAC CCATGGATGA ACATCCCTAC 
AAGTCAAACT ATATTGATGA CAATACGCCC TTTGCTGATT TTAACAAGTT CAACGAATTC 
AGCGGCGATA TGCTAAGCCC CAAAGTCGAG CTAACCGTCA AGGATGAGAC TTACGGAAAG 
ACTTCCAGCA GCAATAGCAG CAGCAGCTTT GCACGTCGAA AAGCCCAGCA ACAGCAAACG 
ACAGATCGTT CGGCGGAGAG TCTGCCCATC TGCCAGCGCT GCAAGGAGGT CTTCTTCAAA 
AAGCAATCCT ATCTGAGGCA TGTGGCCGAG AGCAGTTGTG GCATTCAGGA GTACGATTTC 
AAGTGCAACA TATGCCCCAT GTCCTTCATG ACCACTGAAG AGCTGCAGCG CCACAAGCAA 
CTGCATCGTG CAGACAAGTT CTTCTGCCAC AAATACTGCG GCAAGCATTT CGATACGATA 
GCCGAGTGCG AATCGCACGA GTACATGCAG CACGAGTATG AGAGATTTGT TTGTAATATG 
TGCTCTGGAA CCTTTGCCAC GCGGGAACAG CTGTATGCCC ACTTGCCGCA ACACAAGTTT 
CAGCAGCGTT ACGACTGTCC CATCTGCCGT TTGTGGTATC AAACAGCCGT CGAATTGCAT 
GAGCATCGAC TGTCGGCTCC ATACTTTTGC GGCAAGTACT ATACCAGTCA ACAGCAGCAG 
CTTGCTACGA GCCAGGGGAA TTACAAGCTG CAGGACTGCC ATATGGCCAC CATGGAAATA 
CCCACTGCAC CACTGCACAA GGCAACGCCT TCCAATCCCT CAGCCTTGCC AGCCACAGCT 
GCCTTGAGCT CTCTGTTGCA ACAGCGCCAG GCAAATGCCG ATGGGACTGC GGCCATGTTT 
GCTGCGGCCT CCTCTTCCTC CGCCTCGCTG AAGAGCGAGG TGAGCGTGAA GCTAGAGCGT 
AGCTACAGCA ACTCCACCAG CGAGTCCTCT TACAGCCATC AGGACAACAG CAGCTACAAC 
AATGCCTATG GCAGCGACAA CTCCATCCAT GGCGGAGCAC TGGCCGGACC ACAGGCTCAC 
TCCTCAACGC TGGACGACTC CGAGGATGCC CTGTGTTGTG TGCCCCTGTG CGGCGTCAGA 
AAAAGCACCA GTCCAACGCT GCAGTTCTTT ACGTTTCCAA AGGATGAAAA GTATCTGAAT 
CAGTGGCTAC ACAACCTCAA GATGTTCCAC ATACCAGCGG CGAGCTATGC GACATTTCGC 
ATCTGTAGCA TGCACTTCCC GAAGCGTTGT ATCAATCGGT ATTCGCTGTG CTATTGGGCG 
GTGCCCACCT TCAATCTGGG GCACGACGAT GTGGCCAATC TGTACCAGAA CCGTGAGTTA 
ACCAACACTT TTACCACTGG AGAGGTGGCA CGCTGCAGCA TGCCGCATTG CACCAGCCAG 
CGGGGGGAGA GCAATCTGAA GTTCTACAAT TTCCCCAAGG ACATCAAGAG CCTGATCAAG 
TGGTGCCAGA ACGCCCGCCT GCCAGTGCAG GCCAAGGAGC CGCGTCACTT TTGCAGCCGA 
CACTTTGAGG ATCGCTGTAT TGGCAAGTTC CGACTGAAGC CGTGGGCCGT GCCCACCCTC 
CATCTGGGAG CGCAGTACGG CAAGATCCAT GACAATCCCA AGAATTTGTA TGTGGAGGAG 
AAGCGCTGCT GCCTGAACTT TTGTCGCCGC AGCCGCTCCT CGGACTTTAA CATGTCGCTG 
TATCGTTTCC CCAGAGACGA GGTGCTCCTG CGACGTTGGT GCTATAATTT AAGGCTGGAT 
CCGGGCGTGT ATCGTGGCAA GAATCACAAA ATATGCAGTG CGCATTTTAT CAAGGAAGCT 
TTGGGTCTAA GAAAGCTGTC GCCAGGTGCC GTTCCCACAT TGCATTTGGG TCACAATGAC 
ACCTTTAATA TCTATGAGAA CGAACTGTGG CCACCGCCAT CTCCCACTGG ACAACATGGC 
GGCAGTCACC AGCTTCTCCA GCAACAGCAG ACGTCGCAGC AGCTGTCGCA TCATCATTCG 
TCCCTGCAGC AGCAGCATCA GCCCATGCAT AGCAAATCCT ATCAACGCCA TTCGGCGGCC 
TCCACATCCT CCTCCGCCAG TTCGGCCTCT CATTATGTGG ACCCCGAGAT GAGTGCCTCG 
TATTTGAACC TGTCTGCGGG TGGCTCCTCC GGCGGGATGA ATGCCAGCGA CTGCATGGAT 
GTGTGCTGCG TGCCAAGCTG CGAGAGCAAG CGGCACAACA GCGAGAACAT CACATTCCAC 
ACGATACCGC GCAGGCCAGA GCAGATGCGC AAGTGGTGCC ACAATCTGAA GATACCCGAG 
GAGAAGATGC ACAAGGGCAT GAGGATTTGT AGCCTGCACT TTGAACCATA CTGCATTGGC 
GGCTGCATGC GTCCGTTCGC CGTGCCCACA CTCCATTTGG GGCACGACGA TGAAGACATT 
CACCGCAATC CGGATGTGAT CAAGAAGCTG AACATCCGAG AGACCTGCTG TGTGGCCGTG 
TGCAAACGGA ATCGCGACAG AGACCATGCC AACCTCCATC GTTTCCCCAG CAATGTGGCG 
CTGCTCACCA AGTGGTGTGC GAATCTGCAG CGGACAGTAC CCGATGGCAG CAAACTCTTC 
AACGATGCCA TCTGCGAGGT GCATTTTGAG GATCGTTGTC TGCGCAACAA GAGACTGGAG 
AAGTGGGCTG TGCCCACTCT GATCCTCGGC CACGACGACA TTGCCTATAA GCTGCCGACG 
CCCGAGCAGG TGGCCGAGTT CTATGCCCGT CCCACGGCCC CCAACAATGG CGAGGAGCAG 
GGCGAGTGCT GTGTGGAGAC GTGCAAACGG AACCCGAGTG TGGATGACAT CAAATTGTAT 
CGTCCGCCGG AGGATACTTC GGTGCTGGCC AAATGGGCGC ACAATCTGCA AACGGAGGCC 
ACTGCCCTCA CGAACATGCG GATATGCAAT CTGCACTTTG AGGCCCACTG CATTGGCAAG 
CGGATGCGTC CGTGGGCCAT ACCCACGCTC AATCTGGCTG GAAACATTGA GAATCTGTAC 
GAGAATCCCG AGCATTCGAT GCTGTACAAG CGAAGGACGC ACATCAAACA GAAGGTTCCG 
GTGACAAAGC CCACGTGGGT GCCTCGCTGC TGTCTGCCGC ACTGCCGCAA GGTGCGTGCC 
CTTCACAATG TCCAGCTGTA TCGCTTCCCC AAGCTGAATC GCTCGACGCT GGCCAAGTGG 
GCACACAATC TGCAGGTGCC GCAGGTGGGA AGTGCCCAGC GGCGGGTATG TTCCGCCCAC 
TTTGAGCCGC ATGTTTTGAG TAAAAAGTGC CCGGTGCCGC TGGCGGTGCC CACATTGGAC 
TTGAACTCAC CCGCTGGCCA CAAGATCTAC CAGAATCCGG CCAAGCTGAA GGCCAACAAG 
CTGTGCCTGC AGCGGGTATG CATTGTGGAG AGCTGCAGGA AGACCAGAGC CCAGGGCGTG 
CAGCTCTTCC GTCTGCCTCA CAGCCCCACG CAGCTGAGGA AATGGATGCA CAATATAAGG 
ACACGCCCAA GGGCGGCCAT GAGGAGCCAG TATCGCGTCT GCTCGCGGCA CTTTGAGACG 
CACTCCTTCA ACGGTCGAAG GCTGAGCGCC GGGGCCATTC CCACTTTGGA GTTGGGACAT 
GACGACGATG ATATCTTCCC GAATGAAGCG CAGGCCTTTG CCGATGAGCA CTGCGCTGTG 
GAGGGCTGTG AATCGTCGAA GGAACAGCCC GAAGTGCGGC TCTTCCGCTT CCCCACGGAC 
GACGACGACA TGCTGTGGAA GTGGTGCAAC AACCTCAAGA TGAATCCCGT CGACTGCCTC 
GGTGTGCGGA TCTGCAACAA GCATTTCGAT GCCGATTGCA TTGGACCCAA ACATCTGTAT 
AAGTGGGCCA TACCCACGAT GCTGCTCGGC CACGATGATT CCCAGATCGA GCTCATACTT 
AACCCTAAGC CGGAGGAACG CTACGTGGAT CCCGTGTTCA AGTGCATTGT CCCAACCTGC 
GGGAAGACTC GCCGCTTCGA TGAGGTGCAA ATGAACAGCT TCCCCAAGGA TGCGGATCTG 
TTTCAGCGCT GGCGCCACAA CCTCCGCCTG GAGCATCTGT GCTTCAAGGA GCGCGAGAAA 
TACAAGATCT GCAATGCGCA TTTCGAGGAC ATGTGCATTG GCAAGACGCG TCTGAACATT 
GGTTCCATAC CCACTCTGGA GCTGGGCCAC GAGGAAACGG AGGATCTGTT CAAGGTGAAT 
CCGGAAGATC TGCAGAGCAA TCTGTTTGGG CGTCCCCGTC GGCTGCTAAG AGGATTGAAC 
AATGTGACCA TCAAACAGGA GTTGCCAGAG ACCGAGGAGC AGGACATAAA GCCCGACATA 
AGGACCAACT TTACACAGGT AAAGATTAAG AAATCTCTGG GGGATATTAA GTGCTGTGTG 
CACACGTGTG GACGCAGTCG TTTGGAGCAT GGGGCACGTC TCTTTCCCTT CCCCACGGGC 
AAGCAACAGC ACCTCAAGTG GCGCCACAAT CTGCGCCTGG AGCCCGACGA AGTGGACAAA 
ACCACGCGCG TCTGCAGCGC ACACTTCAAC AGGCGCTGCA TCGATGGAAA GCATCTTAGG 
GGATGGGCCA TGCCCACACA GCAGTTGGGC CACCAAGAAC AGCCTATATA CGAGAATCCC 
AAGAATATTC CCGGCTTCTT TACGCCCACC TGTGCGCTGG GCCACTGCCG CAAGCGGCGG 
AGCATTGACA ATGATTTGCG CACGTATCGT TATCCGCGGA GCGAGGATCT CCTCGAGAAG 
TGGCGCGCAA ATCTCAGACT ATCGCTGGAT CAGTGTCGTG GCAGGATCTG TGCGGATCAC 
TTTGAGCCGC AGGTGAGGGG CAAACTGAAG TTGAAGACGG GAGCAGTACC CACGCTAAAA 
CTGGGCCATG AGGAGGCTTT GATGTACGAC AATGAGGCTA TAAAGGCTGG AGTGGCCGAA 
GAGGAGGCTG GCAGTCCGCC GGCATCGCCT CTGGTGACAC CCAAAACGGA AGTTCTGGAC 
GAAGAGGAGC GCGAGGATGA TGAGGAGGAG GAGGAGAACC CCGAAGAAGA GCAGCAGGAA 
AACCATGATG AGGAGAAGGA TGAACACGAG GATGACACGC CCGAGGGAGC AGAGCAGCTG 
GGCGATGAGG ATGACGACGA GGATCCAGGC AACTATTTTG ATCCGTTGGA GCTGGTGGAG 
ACGTATGCAG AGCATCCCAG CGACGATGAC AACAGCCACG AGGAAGCAGA CGATGCCAGA 
GAGGAGGATG AGGAGGAAGA GGAGGAGGCA GAAACTCTCT TGCCTGATAC ACCACCCAAC 
GTAGCAGCAG CAGCAGTCCT TCGCGTGCCG AAACCATGGG AAAGACCTCT CGCAGTAGTG 
CCTCGCCGAG AGAAGCGTCC GAATAACGTG GATCCCATCT GCTGCCTCAA ACACTGCCGC 
AAGGAACGCT CCGCCATGTA TCTGCTGAGC ACATTTGGCT TTCCAAAGGA CCAGCAGCTG 
CTGCTCAAGT GGTGCGCCAA CCTCCAAATG GATCCCTCGA GCTGCATTGG TCGCGTCTGC 
ATCGAACACT TCCAGTCCGA GGTTCTGGGC ACGCGAAAAC TCAAACAGAA TGCGGTGCCC 
ACCCTCAATG TGGGTCACGA TGTGCCACTG CGCTACAGCT GCAACGGCCA AGAGAGGCCT 
CAGGCAGCAG CGGCGGCGGC CGCCACGAAC AGCTTCCCCG ACGAAATGCC ACAACATTCG 
GTTTTTCGGC TTTGGAGCCT GAAACACTGC CGCAAGAGGA AAGTGTCGGA GAGTCCAGTT 
CCAGCAGCAA TCAAGACGGA GGAGCAGATG CAGATGGAGA TGGAGGTGGA GACTAAGCCA 
AAGATATGCT GCCTCTCCAG TTGTGGCAAT GTGGAGGGCT ACGGCCCGGG CGGGTACTTT 
CAGCCGCTGC CCCAGGACCA AAGGATACTG AAAAAATGGC AGCACAACCT GAAGCTACCA 
TCTGTCAATC CCGATTCGGA TCTTCGTGAC TTTCGCCTGT GCATGGAGCA CTTTGAGCCG 
CATCAAATCG AGAACGGAGC CCCAGTGAAG ATGGCCGTTC CGACCCTCAA GCTTGGGCAC 
TCCAGTCCGA ATATCTTTAA GAACAGCGAG AGCACGCTGC CGGGATGCCT GTGGCCCTCG 
TGTCCGCCCA ATCGCAAGAT CTGCTACGAT CTGCCCGACA ATGAGGCCGT TCGAGCGGCC 
TGGCTGTCGT ATGTGCGGCT GCCGCCGGAC AGCCAGGGTC GTCTGTGTGG TCTGCACTTT 
CTGCAGCTGT ACGAGGAGGT GGATCTGCCA GGAGATGTAC CCGAAGCGGT GCTCGAGCGA 
CTGCAGGGTA TCTATGATCA GGCCTCCATC TCGCTGAAGT TTCAGTGCTC GGTGCAGGGA 
TGTGGCTCCA AGTACAAGCA GGATACGCAT TTGACAAAGC TGCCACGGGA CGCGGAACTG 
CTGGCCAAGT GGCTGCACAA CACCAGGATC TCCTACGATC GCTCCTTGCA TTTTAGCTAC 
CGCATTTGTC TATTGCACTT TGAGGCGTTC TGCTTGAATG GCGTGCGCCC GCAGACCTGG 
GCCATACCTA CGCTCCAGCT AAATCACGAC GGAGAGATCT ACCAAAATAC CGCCAAGCAG 
GAGATCCCAG AGATGCCCCT GAAGCAGGAG ATCCTCGAGA ATCCCGTGAA GCAGGAGAAA 
TCGCACTGTG GGAGAATCTC CAGCCTGAGT CTCTCCATCC CCCTGCACAT CAAGACGGAA 
CAGGGTCCTG TCCTGCGACC TCGAGGCACT TGGGGCACAT CTTCTCAGAG CAGTCCCTGC 
CTGAGCGCCA GCTCCAGTCC CCGCCTGAAA AACAGAATCT GCTGCGTCTC CGATTGCGGA 
GAGTACGCCA AATCCCAGCG GCTGTACCGC TTTCCCACCG CCGAACCGGC ACTGCTCAAG 
TGGCTGGTTA ATACCCAGCA AAAACCGGGA CTCGTGGACA TCCAGAACCT GTTTGTGTGC 
CAGCTTCATT TCGAGGCGGA CGCCATTAAC CAAACGCAGC TCAGGAGCTG GGCCGTGCCC 
ACACTGAGGC TGGGCCACGA TGGGCATGTC ATACCAAATG CCAGACACAA TGGGAACATA 
GCCAACAGCC AGGAAACGGA GCAGGCCATG GAGTTCATTC GGGCCAACTA CTGCGCCGTG 
TTGAGCTGCT TCCAGCCGAA AGGAGATGGT GTACGCTTCT ACAAGTATCC CAGCGACATT 
GCCATGGCGC GCAGGTGGGC CACGAATCTC AAGCATCGCT CCATGCAGGC CAGCAGTCAT 
GGCTTCCTGG TTTGCCAGTC CCACTTTGCA GCCGACTGTT TTGATCCGGA GACGGGAGAC 
CTGCGCGAGG ACGCCGTACC CGTGGCCACA CTCGCAGAGA ACGTGAAATC AGAGGGCCTG 
CTGCTCCGTT GTCTGGTAAG GGGTTGCTCT ACGGATAACT CTGAAAAAGG ACTGCTGTTC 
AAGGTGCCAA AAAAGAATCG TGTACGGGAT GTGTGGGCCC ACAATCTGTG GATGCATCCG 
ATAGAGCTGA TGGGCGAGCA CTACATCTGC GATCGACATT TCGAGGCGCA TTGCGTGAAT 
GAACACAAAT TACTCCACGC GGGCTCAGTG CCAACCCTCC ACCTGGGACA CAACGAACCG 
CTGGAACTAC TGCCCAATCC CCAGACCTTC CAGGACTTCC CTGAGGAGTG CGAGTGCTGT 
GTGCCCGGCT GTGGACGCAC CAATCGGAAG GAGGAGGATC TGCAGTTTAG CAAATTTCCC 
AAGTGGCGAG TCCTGTATGA AAAGTGGCTG CACAACTTCC GCCTCGAAGT GCCCAAGGAG 
CAGCGCATCG GGACGCTGAG AGTGTGTCAC ATGCACTTTG AGGAGAGCTG CTACGATGGC 
CAGAACGTGC GCAGGGGAGC TATGCCCACC CTAGAGCTGG GACACTCGCA TCCAGACATT 
TATCGCTCCG ACAAGGGATC GCTGTGGAAG AAGGTTCACA AGAGACTCAC CGATTGTTGC 
TATCCCGATT GCTACGAGGA GTGTCACAAG GCCAACACGA ATCGCACGAC CTACGATCTG 
CCCAGCGATG GGCCACTGCG CGAGTCCTGG CAGCAGCACA TGGCCATCCC TGCCAGCGGT 
GAGGATGGCT CCTCAGTGCT AAAGCTCTGT GCCCTACACT ACATCATTAT GTACGAGCAC 
AGCGAACAGA GCTTCCCAGA ACACGGACCG AATCTACTGC TGGACAAGAA CTACGAGCAC 
GCCCGTCAGT TGGCGTATCT GCGGCGCTTC TTGTGTGCGG TACAGGGCTG TCGTTATTTG 
CAGCCGCGGG ATGGGGGTCC GATGCACGGA ATACCCCGGC GGAGGGAGAT CCTTCGGATG 
TGGGTGGAGA ATGCCCAGCT GCGGCTGAAC GAGCACGAAA TTTACATGAC GAAGCTGTGC 
AGCAAACATT TTGAGGCCCA CTGCCTGTTC GAGGGTAAAA AATGCTATCC CTGGAGCGTG 
CCAACGCTCC ATCTGCCAGA GGCGCAGCCC GGGCAGGTGC TCCACCAGAA TCCCACCAAG 
GAGGAGTGGC AGGAAATGAA ACAGAGAATG AAATTGGAAG AGCAGACGCC GAAGACGGAA 
GAGCAGGCAG ATGGACTACT AATGGAACCC TATGTGAAGA TGGAACCCCA CGACGACGAG 
TCACAAACGG AGTCGGAATT GCTGATAAAT GAGAGCACAC TGGACTCTCA AGAACTCTCT 
CAAGACTTTC CAACACAAGA TCCAATCGAA ATGCCCGCCC TGGAGGTGCT CCTAGAGGTG 
AGCCATGTCG AGAAGCTGGA TAGCTACGAG AGGAATGAAT ACCCTGCGGA TACCTCTGCC 
AACACAAATT CTCCGAACAA ACGTTTCCGC CATCAGTACA GTGCCCACAA GTGTAGTGTC 
GAGGGATGTC GCGTGTCGCT CGAGGACCTT GATGGGAATC TGAAGCTGCA CAAGCTACCC 
AGCTCCACGG AGGCGGCCGG GAAGTGGCTG TACAACATTC ACGTGGAGAT AGAGGATAAA 
TGGCGGATAC GCGTCTGCAG CCATCACTTC GACAGGCAGT GCCTCAATGG TTCAAGGCTC 
AGGAGGGGAT CGATGCCCAC TCTGCTGCTG GGGCCGCGTG TTCCAGAGAC TATCCATCAG 
AATGAGTTTG CGCAGCTACA ATTGGACGAT GCACCAGCCC ATAATGGCAA TACATTGGAG 
CGATCCATTG GAAAGGTTGC GCAGATATGC GTTCCACGTC CGTCGCCGCC GCGTAAGTCG 
AGCAAATTCT GCCAGATCGA GGGATGTTCG AACCATCTGA CCAGCGAGAA TATGACGCTA 
CACAAGTTCC CGCACTCGTC GTGGATCTGC ACCAAATGGC AGCACAACAC ACAGGTGCCG 
TTCGATCCGG AGTACCGCTG GCGCTATCGC ATCTGCAGCG CCCACTTCCA TCCCGTGTGT 
ATGGTCAATA TGCGGCTGCT GCATGGCAGT GTGCCCACCC TGAAGCTTGG TCCACGGGCA 
CCCGGCGAAC TCTTTGACAG CGACTTTGAG GCCATAAACA TAAAGATTGA AAAAATGGAG 
AAGATGGAGA GGAAATCTGA GGCTCTGAGA AGCACCACTG GAGATAGATA TCCCACCATG 
CAGGCCATGG GGGAGAAGAA GTTCAAGACT GAGGAGCTGG AAGATGGAAT GGAGGAGGAG 
GATGACATGC TCTGCCTGGA GCCAGAGATG CAGCTATACG AAGATCAGGA AGAACAGCAA 
CAGAAGCCAA AGATAAATCT TGGAGTCCCC AATGGCGGCT GGAAAACGGA ACTCCGTTTG 
CCATCGAAGG GTAGGGTGGC GTTCAATCCG GTGAGATCTG GCTACGACAA GTGCTCGCTG 
ATGCATTGCC AGCGCCAGAG ATCGAAGCAC GGCGTCCACA TCTACAAGTT CCCCCGATCG 
CAGGAGCACC AACAGCGATG GATGCACAAT CTGCGCATAC GCTACGATGA GAAGCGACCC 
TGGAAGTTTA TGGTCTGCAG CGTGCACTTT GAACCGCATT GCATACGGCT GCGGAAGCTG 
CGGCCCTGGG CAGTTCCCAC GCTAGAGCTG GGAGACAATG TGCCCGAGGA CATCTATACG 
AACGAGCAGT GCCAGATGTT TGCCAGTGGA CAGGGAGGAG AGATCAATGG CATCGATAGC 
GATGAGGTGG AGGCGGAGCC GGAGGCAGAA GGGGAGAGCG ATGGGAATGA TGAGGATGGC 
CTGCAGGAGG ACGAGGATGA GGAGACAGAC GACCAGGAGC CCATCGCCAA GAAGCGTCGT 
CGCTCGCGGC TGGATGCCGT CTGGCCTCCC GGCCAGGCGC CACCATGGAA GGTGAAACAA 
TGCTGTCTTC CCTACTGTCG CAGTCCACGC GGCGAGGGCA TCAAGCTGTT TCGACTGCCC 
AACAAAGTCA ACTCCATCCG CAACTGGGAG CTGGCCACGG GCATGAAGTT CAAGGAGTCG 
CAGCGCAACA CGAGACTCAT CTGCAGCCGC CACTTTGATC CGGAGCTGAT CGGAGTGCGT 
CGTCTGATGC GCAATGCCAT TCCCACCAGG CATCTGGGAC CCACGGGCGA TGTAAAGCCA 
GTGATGGCTC CACCGACAGC TGGTCCCAAA TGCTGTATGG CAGATTGTAC CTATGATGTG 
GCGGATGTGA AGCTGCACAA GTTTCCCAGC AATCCCAAAC TCCTGAAGGA GTGGTGCCAG 
GCATTAAGGG TCACGGATAT GCAAAGGTAT CGCGGCAAGC ACATTTGCTC CGCCCATCTA 
CCTGTCCACG AGGCCGTACA GTGCATTGTT TGTGGCGCGG AGAAAGCACC CCTGCTGCCG 
ATGCTTAATT TTCCCGCTAA CCGGAATCAG CGCGCCAAAT GGTGCTACAA TCTTAAGATC 
GAAACGATAC CCAAGTGGGA CATATCCAAG CACATTTGCT GTAAACACTT TGAGCCATAT 
TGTTTTGCAG AGGCGGGTCT CCTAAAGCCA GAGGCGGCAC CCACGCTGCA TTTGAATCAC 
AATGATACAA ATATATTCCT TAACGATTGT GCCATAAATC CTGCCTACAG CGTATGCGTG 
AAGGATGAAC CCATGGACAA TCAGGTCCTG TCATTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036900.1
CDS	1..11142
Translation	MELKWVEVVSMSEPMDEHPYKSNYIDDNTPFADFNKFNEFSGDMLSPKVELTVKDETYGKTSSSNSSSSFARRKAQQQQTTDRSAESLPICQRCKEVFFKKQSYLRHVAESSCGIQEYDFKCNICPMSFMTTEELQRHKQLHRADKFFCHKYCGKHFDTIAECESHEYMQHEYERFVCNMCSGTFATREQLYAHLPQHKFQQRYDCPICRLWYQTAVELHEHRLSAPYFCGKYYTSQQQQLATSQGNYKLQDCHMATMEIPTAPLHKATPSNPSALPATAALSSLLQQRQANADGTAAMFAAASSSSASLKSEVSVKLERSYSNSTSESSYSHQDNSSYNNAYGSDNSIHGGALAGPQAHSSTLDDSEDALCCVPLCGVRKSTSPTLQFFTFPKDEKYLNQWLHNLKMFHIPAASYATFRICSMHFPKRCINRYSLCYWAVPTFNLGHDDVANLYQNRELTNTFTTGEVARCSMPHCTSQRGESNLKFYNFPKDIKSLIKWCQNARLPVQAKEPRHFCSRHFEDRCIGKFRLKPWAVPTLHLGAQYGKIHDNPKNLYVEEKRCCLNFCRRSRSSDFNMSLYRFPRDEVLLRRWCYNLRLDPGVYRGKNHKICSAHFIKEALGLRKLSPGAVPTLHLGHNDTFNIYENELWPPPSPTGQHGGSHQLLQQQQTSQQLSHHHSSLQQQHQPMHSKSYQRHSAASTSSSASSASHYVDPEMSASYLNLSAGGSSGGMNASDCMDVCCVPSCESKRHNSENITFHTIPRRPEQMRKWCHNLKIPEEKMHKGMRICSLHFEPYCIGGCMRPFAVPTLHLGHDDEDIHRNPDVIKKLNIRETCCVAVCKRNRDRDHANLHRFPSNVALLTKWCANLQRTVPDGSKLFNDAICEVHFEDRCLRNKRLEKWAVPTLILGHDDIAYKLPTPEQVAEFYARPTAPNNGEEQGECCVETCKRNPSVDDIKLYRPPEDTSVLAKWAHNLQTEATALTNMRICNLHFEAHCIGKRMRPWAIPTLNLAGNIENLYENPEHSMLYKRRTHIKQKVPVTKPTWVPRCCLPHCRKVRALHNVQLYRFPKLNRSTLAKWAHNLQVPQVGSAQRRVCSAHFEPHVLSKKCPVPLAVPTLDLNSPAGHKIYQNPAKLKANKLCLQRVCIVESCRKTRAQGVQLFRLPHSPTQLRKWMHNIRTRPRAAMRSQYRVCSRHFETHSFNGRRLSAGAIPTLELGHDDDDIFPNEAQAFADEHCAVEGCESSKEQPEVRLFRFPTDDDDMLWKWCNNLKMNPVDCLGVRICNKHFDADCIGPKHLYKWAIPTMLLGHDDSQIELILNPKPEERYVDPVFKCIVPTCGKTRRFDEVQMNSFPKDADLFQRWRHNLRLEHLCFKEREKYKICNAHFEDMCIGKTRLNIGSIPTLELGHEETEDLFKVNPEDLQSNLFGRPRRLLRGLNNVTIKQELPETEEQDIKPDIRTNFTQVKIKKSLGDIKCCVHTCGRSRLEHGARLFPFPTGKQQHLKWRHNLRLEPDEVDKTTRVCSAHFNRRCIDGKHLRGWAMPTQQLGHQEQPIYENPKNIPGFFTPTCALGHCRKRRSIDNDLRTYRYPRSEDLLEKWRANLRLSLDQCRGRICADHFEPQVRGKLKLKTGAVPTLKLGHEEALMYDNEAIKAGVAEEEAGSPPASPLVTPKTEVLDEEEREDDEEEEENPEEEQQENHDEEKDEHEDDTPEGAEQLGDEDDDEDPGNYFDPLELVETYAEHPSDDDNSHEEADDAREEDEEEEEEAETLLPDTPPNVAAAAVLRVPKPWERPLAVVPRREKRPNNVDPICCLKHCRKERSAMYLLSTFGFPKDQQLLLKWCANLQMDPSSCIGRVCIEHFQSEVLGTRKLKQNAVPTLNVGHDVPLRYSCNGQERPQAAAAAAATNSFPDEMPQHSVFRLWSLKHCRKRKVSESPVPAAIKTEEQMQMEMEVETKPKICCLSSCGNVEGYGPGGYFQPLPQDQRILKKWQHNLKLPSVNPDSDLRDFRLCMEHFEPHQIENGAPVKMAVPTLKLGHSSPNIFKNSESTLPGCLWPSCPPNRKICYDLPDNEAVRAAWLSYVRLPPDSQGRLCGLHFLQLYEEVDLPGDVPEAVLERLQGIYDQASISLKFQCSVQGCGSKYKQDTHLTKLPRDAELLAKWLHNTRISYDRSLHFSYRICLLHFEAFCLNGVRPQTWAIPTLQLNHDGEIYQNTAKQEIPEMPLKQEILENPVKQEKSHCGRISSLSLSIPLHIKTEQGPVLRPRGTWGTSSQSSPCLSASSSPRLKNRICCVSDCGEYAKSQRLYRFPTAEPALLKWLVNTQQKPGLVDIQNLFVCQLHFEADAINQTQLRSWAVPTLRLGHDGHVIPNARHNGNIANSQETEQAMEFIRANYCAVLSCFQPKGDGVRFYKYPSDIAMARRWATNLKHRSMQASSHGFLVCQSHFAADCFDPETGDLREDAVPVATLAENVKSEGLLLRCLVRGCSTDNSEKGLLFKVPKKNRVRDVWAHNLWMHPIELMGEHYICDRHFEAHCVNEHKLLHAGSVPTLHLGHNEPLELLPNPQTFQDFPEECECCVPGCGRTNRKEEDLQFSKFPKWRVLYEKWLHNFRLEVPKEQRIGTLRVCHMHFEESCYDGQNVRRGAMPTLELGHSHPDIYRSDKGSLWKKVHKRLTDCCYPDCYEECHKANTNRTTYDLPSDGPLRESWQQHMAIPASGEDGSSVLKLCALHYIIMYEHSEQSFPEHGPNLLLDKNYEHARQLAYLRRFLCAVQGCRYLQPRDGGPMHGIPRRREILRMWVENAQLRLNEHEIYMTKLCSKHFEAHCLFEGKKCYPWSVPTLHLPEAQPGQVLHQNPTKEEWQEMKQRMKLEEQTPKTEEQADGLLMEPYVKMEPHDDESQTESELLINESTLDSQELSQDFPTQDPIEMPALEVLLEVSHVEKLDSYERNEYPADTSANTNSPNKRFRHQYSAHKCSVEGCRVSLEDLDGNLKLHKLPSSTEAAGKWLYNIHVEIEDKWRIRVCSHHFDRQCLNGSRLRRGSMPTLLLGPRVPETIHQNEFAQLQLDDAPAHNGNTLERSIGKVAQICVPRPSPPRKSSKFCQIEGCSNHLTSENMTLHKFPHSSWICTKWQHNTQVPFDPEYRWRYRICSAHFHPVCMVNMRLLHGSVPTLKLGPRAPGELFDSDFEAINIKIEKMEKMERKSEALRSTTGDRYPTMQAMGEKKFKTEELEDGMEEEDDMLCLEPEMQLYEDQEEQQQKPKINLGVPNGGWKTELRLPSKGRVAFNPVRSGYDKCSLMHCQRQRSKHGVHIYKFPRSQEHQQRWMHNLRIRYDEKRPWKFMVCSVHFEPHCIRLRKLRPWAVPTLELGDNVPEDIYTNEQCQMFASGQGGEINGIDSDEVEAEPEAEGESDGNDEDGLQEDEDEETDDQEPIAKKRRRSRLDAVWPPGQAPPWKVKQCCLPYCRSPRGEGIKLFRLPNKVNSIRNWELATGMKFKESQRNTRLICSRHFDPELIGVRRLMRNAIPTRHLGPTGDVKPVMAPPTAGPKCCMADCTYDVADVKLHKFPSNPKLLKEWCQALRVTDMQRYRGKHICSAHLPVHEAVQCIVCGAEKAPLLPMLNFPANRNQRAKWCYNLKIETIPKWDISKHICCKHFEPYCFAEAGLLKPEAAPTLHLNHNDTNIFLNDCAINPAYSVCVKDEPMDNQVLSLV

Target ORF information:

RefSeq Version	XM_015181414.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10453), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181414.1

ORF Insert Sequence:

ATGGAATTGA AATGGGTTGA GGTTGTGTCT ATGTCGGAAC CCATGGATGA ACATCCCTAC 
AAGTCAAACT ATATTGATGA CAATACGCCC TTTGCTGATT TTAACAAGTT CAACGAATTC 
AGCGGCGATA TGCTAAGCCC CAAAGTCGAG CTAACCGTCA AGGATGAGAC TTACGGAAAG 
ACTTCCAGCA GCAATAGCAG CAGCAGCTTT GCACGTCGAA AAGCCCAGCA ACAGCAAACG 
ACAGATCGTT CGGCGGAGAG TCTGCCCATC TGCCAGCGCT GCAAGGAGGT CTTCTTCAAA 
AAGCAATCCT ATCTGAGGCA TGTGGCCGAG AGCAGTTGTG GCATTCAGGA GTACGATTTC 
AAGTGCAACA TATGCCCCAT GTCCTTCATG ACCACTGAAG AGCTGCAGCG CCACAAGCAA 
CTGCATCGTG CAGACAAGTT CTTCTGCCAC AAATACTGCG GCAAGCATTT CGATACGATA 
GCCGAGTGCG AATCGCACGA GTACATGCAG CACGAGTATG AGAGATTTGT TTGTAATATG 
TGCTCTGGAA CCTTTGCCAC GCGGGAACAG CTGTATGCCC ACTTGCCGCA ACACAAGTTT 
CAGCAGCGTT ACGACTGTCC CATCTGCCGT TTGTGGTATC AAACAGCCGT CGAATTGCAT 
GAGCATCGAC TGTCGGCTCC ATACTTTTGC GGCAAGTACT ATACCAGTCA ACAGCAGCAG 
CTTGCTACGA GCCAGGGGAA TTACAAGCTG CAGGACTGCC ATATGGCCAC CATGGAAATA 
CCCACTGCAC CACTGCACAA GGCAACGCCT TCCAATCCCT CAGCCTTGCC AGCCACAGCT 
GCCTTGAGCT CTCTGTTGCA ACAGCGCCAG GCAAATGCCG ATGGGACTGC GGCCATGTTT 
GCTGCGGCCT CCTCTTCCTC CGCCTCGCTG AAGAGCGAGG TGAGCGTGAA GCTAGAGCGT 
AGCTACAGCA ACTCCACCAG CGAGTCCTCT TACAGCCATC AGGACAACAG CAGCTACAAC 
AATGCCTATG GCAGCGACAA CTCCATCCAT GGCGGAGCAC TGGCCGGACC ACAGGCTCAC 
TCCTCAACGC TGGACGACTC CGAGGATGCC CTGTGTTGTG TGCCCCTGTG CGGCGTCAGA 
AAAAGCACCA GTCCAACGCT GCAGTTCTTT ACGTTTCCAA AGGATGAAAA GTATCTGAAT 
CAGTGGCTAC ACAACCTCAA GATGTTCCAC ATACCAGCGG CGAGCTATGC GACATTTCGC 
ATCTGTAGCA TGCACTTCCC GAAGCGTTGT ATCAATCGGT ATTCGCTGTG CTATTGGGCG 
GTGCCCACCT TCAATCTGGG GCACGACGAT GTGGCCAATC TGTACCAGAA CCGTGAGTTA 
ACCAACACTT TTACCACTGG AGAGGTGGCA CGCTGCAGCA TGCCGCATTG CACCAGCCAG 
CGGGGGGAGA GCAATCTGAA GTTCTACAAT TTCCCCAAGG ACATCAAGAG CCTGATCAAG 
TGGTGCCAGA ACGCCCGCCT GCCAGTGCAG GCCAAGGAGC CGCGTCACTT TTGCAGCCGA 
CACTTTGAGG ATCGCTGTAT TGGCAAGTTC CGACTGAAGC CGTGGGCCGT GCCCACCCTC 
CATCTGGGAG CGCAGTACGG CAAGATCCAT GACAATCCCA AGAATTTGTA TGTGGAGGAG 
AAGCGCTGCT GCCTGAACTT TTGTCGCCGC AGCCGCTCCT CGGACTTTAA CATGTCGCTG 
TATCGTTTCC CCAGAGACGA GGTGCTCCTG CGACGTTGGT GCTATAATTT AAGGCTGGAT 
CCGGGCGTGT ATCGTGGCAA GAATCACAAA ATATGCAGTG CGCATTTTAT CAAGGAAGCT 
TTGGGTCTAA GAAAGCTGTC GCCAGGTGCC GTTCCCACAT TGCATTTGGG TCACAATGAC 
ACCTTTAATA TCTATGAGAA CGAACTGTGG CCACCGCCAT CTCCCACTGG ACAACATGGC 
GGCAGTCACC AGCTTCTCCA GCAACAGCAG ACGTCGCAGC AGCTGTCGCA TCATCATTCG 
TCCCTGCAGC AGCAGCATCA GCCCATGCAT AGCAAATCCT ATCAACGCCA TTCGGCGGCC 
TCCACATCCT CCTCCGCCAG TTCGGCCTCT CATTATGTGG ACCCCGAGAT GAGTGCCTCG 
TATTTGAACC TGTCTGCGGG TGGCTCCTCC GGCGGGATGA ATGCCAGCGA CTGCATGGAT 
GTGTGCTGCG TGCCAAGCTG CGAGAGCAAG CGGCACAACA GCGAGAACAT CACATTCCAC 
ACGATACCGC GCAGGCCAGA GCAGATGCGC AAGTGGTGCC ACAATCTGAA GATACCCGAG 
GAGAAGATGC ACAAGGGCAT GAGGATTTGT AGCCTGCACT TTGAACCATA CTGCATTGGC 
GGCTGCATGC GTCCGTTCGC CGTGCCCACA CTCCATTTGG GGCACGACGA TGAAGACATT 
CACCGCAATC CGGATGTGAT CAAGAAGCTG AACATCCGAG AGACCTGCTG TGTGGCCGTG 
TGCAAACGGA ATCGCGACAG AGACCATGCC AACCTCCATC GTTTCCCCAG CAATGTGGCG 
CTGCTCACCA AGTGGTGTGC GAATCTGCAG CGGACAGTAC CCGATGGCAG CAAACTCTTC 
AACGATGCCA TCTGCGAGGT GCATTTTGAG GATCGTTGTC TGCGCAACAA GAGACTGGAG 
AAGTGGGCTG TGCCCACTCT GATCCTCGGC CACGACGACA TTGCCTATAA GCTGCCGACG 
CCCGAGCAGG TGGCCGAGTT CTATGCCCGT CCCACGGCCC CCAACAATGG CGAGGAGCAG 
GGCGAGTGCT GTGTGGAGAC GTGCAAACGG AACCCGAGTG TGGATGACAT CAAATTGTAT 
CGTCCGCCGG AGGATACTTC GGTGCTGGCC AAATGGGCGC ACAATCTGCA AACGGAGGCC 
ACTGCCCTCA CGAACATGCG GATATGCAAT CTGCACTTTG AGGCCCACTG CATTGGCAAG 
CGGATGCGTC CGTGGGCCAT ACCCACGCTC AATCTGGCTG GAAACATTGA GAATCTGTAC 
GAGAATCCCG AGCATTCGAT GCTGTACAAG CGAAGGACGC ACATCAAACA GAAGGTTCCG 
GTGACAAAGC CCACGTGGGT GCCTCGCTGC TGTCTGCCGC ACTGCCGCAA GGTGCGTGCC 
CTTCACAATG TCCAGCTGTA TCGCTTCCCC AAGCTGAATC GCTCGACGCT GGCCAAGTGG 
GCACACAATC TGCAGGTGCC GCAGGTGGGA AGTGCCCAGC GGCGGGTATG TTCCGCCCAC 
TTTGAGCCGC ATGTTTTGAG TAAAAAGTGC CCGGTGCCGC TGGCGGTGCC CACATTGGAC 
TTGAACTCAC CCGCTGGCCA CAAGATCTAC CAGAATCCGG CCAAGCTGAA GGCCAACAAG 
CTGTGCCTGC AGCGGGTATG CATTGTGGAG AGCTGCAGGA AGACCAGAGC CCAGGGCGTG 
CAGCTCTTCC GTCTGCCTCA CAGCCCCACG CAGCTGAGGA AATGGATGCA CAATATAAGG 
ACACGCCCAA GGGCGGCCAT GAGGAGCCAG TATCGCGTCT GCTCGCGGCA CTTTGAGACG 
CACTCCTTCA ACGGTCGAAG GCTGAGCGCC GGGGCCATTC CCACTTTGGA GTTGGGACAT 
GACGACGATG ATATCTTCCC GAATGAAGCG CAGGCCTTTG CCGATGAGCA CTGCGCTGTG 
GAGGGCTGTG AATCGTCGAA GGAACAGCCC GAAGTGCGGC TCTTCCGCTT CCCCACGGAC 
GACGACGACA TGCTGTGGAA GTGGTGCAAC AACCTCAAGA TGAATCCCGT CGACTGCCTC 
GGTGTGCGGA TCTGCAACAA GCATTTCGAT GCCGATTGCA TTGGACCCAA ACATCTGTAT 
AAGTGGGCCA TACCCACGAT GCTGCTCGGC CACGATGATT CCCAGATCGA GCTCATACTT 
AACCCTAAGC CGGAGGAACG CTACGTGGAT CCCGTGTTCA AGTGCATTGT CCCAACCTGC 
GGGAAGACTC GCCGCTTCGA TGAGGTGCAA ATGAACAGCT TCCCCAAGGA TGCGGATCTG 
TTTCAGCGCT GGCGCCACAA CCTCCGCCTG GAGCATCTGT GCTTCAAGGA GCGCGAGAAA 
TACAAGATCT GCAATGCGCA TTTCGAGGAC ATGTGCATTG GCAAGACGCG TCTGAACATT 
GGTTCCATAC CCACTCTGGA GCTGGGCCAC GAGGAAACGG AGGATCTGTT CAAGGTGAAT 
CCGGAAGATC TGCAGAGCAA TCTGTTTGGG CGTCCCCGTC GGCTGCTAAG AGGATTGAAC 
AATGTGACCA TCAAACAGGA GTTGCCAGAG ACCGAGGAGC AGGACATAAA GCCCGACATA 
AGGACCAACT TTACACAGGT AAAGATTAAG AAATCTCTGG GGGATATTAA GTGCTGTGTG 
CACACGTGTG GACGCAGTCG TTTGGAGCAT GGGGCACGTC TCTTTCCCTT CCCCACGGGC 
AAGCAACAGC ACCTCAAGTG GCGCCACAAT CTGCGCCTGG AGCCCGACGA AGTGGACAAA 
ACCACGCGCG TCTGCAGCGC ACACTTCAAC AGGCGCTGCA TCGATGGAAA GCATCTTAGG 
GGATGGGCCA TGCCCACACA GCAGTTGGGC CACCAAGAAC AGCCTATATA CGAGAATCCC 
AAGAATATTC CCGGCTTCTT TACGCCCACC TGTGCGCTGG GCCACTGCCG CAAGCGGCGG 
AGCATTGACA ATGATTTGCG CACGTATCGT TATCCGCGGA GCGAGGATCT CCTCGAGAAG 
TGGCGCGCAA ATCTCAGACT ATCGCTGGAT CAGTGTCGTG GCAGGATCTG TGCGGATCAC 
TTTGAGCCGC AGGTGAGGGG CAAACTGAAG TTGAAGACGG GAGCAGTACC CACGCTAAAA 
CTGGGCCATG AGGAGGCTTT GATGTACGAC AATGAGGCTA TAAAGGCTGG AGTGGCCGAA 
GAGGAGGCTG GCAGTCCGCC GGCATCGCCT CTGGTGACAC CCAAAACGGA AGTTCTGGAC 
GAAGAGGAGC GCGAGGATGA TGAGGAGGAG GAGGAGAACC CCGAAGAAGA GCAGCAGGAA 
AACCATGATG AGGAGAAGGA TGAACACGAG GATGACACGC CCGAGGGAGC AGAGCAGCTG 
GGCGATGAGG ATGACGACGA GGATCCAGGC AACTATTTTG ATCCGTTGGA GCTGGTGGAG 
ACGTATGCAG AGCATCCCAG CGACGATGAC AACAGCCACG AGGAAGCAGA CGATGCCAGA 
GAGGAGGATG AGGAGGAAGA GGAGGAGGCA GAAACTCTCT TGCCTGATAC ACCACCCAAC 
GTAGCAGCAG CAGCAGTCCT TCGCGTGCCG AAACCATGGG AAAGACCTCT CGCAGTAGTG 
CCTCGCCGAG AGAAGCGTCC GAATAACGTG GATCCCATCT GCTGCCTCAA ACACTGCCGC 
AAGGAACGCT CCGCCATGTA TCTGCTGAGC ACATTTGGCT TTCCAAAGGA CCAGCAGCTG 
CTGCTCAAGT GGTGCGCCAA CCTCCAAATG GATCCCTCGA GCTGCATTGG TCGCGTCTGC 
ATCGAACACT TCCAGTCCGA GGTTCTGGGC ACGCGAAAAC TCAAACAGAA TGCGGTGCCC 
ACCCTCAATG TGGGTCACGA TGTGCCACTG CGCTACAGCT GCAACGGCCA AGAGAGGCCT 
CAGGCAGCAG CGGCGGCGGC CGCCACGAAC AGCTTCCCCG ACGAAATGCC ACAACATTCG 
GTTTTTCGGC TTTGGAGCCT GAAACACTGC CGCAAGAGGA AAGTGTCGGA GAGTCCAGTT 
CCAGCAGCAA TCAAGACGGA GGAGCAGATG CAGATGGAGA TGGAGGTGGA GACTAAGCCA 
AAGATATGCT GCCTCTCCAG TTGTGGCAAT GTGGAGGGCT ACGGCCCGGG CGGGTACTTT 
CAGCCGCTGC CCCAGGACCA AAGGATACTG AAAAAATGGC AGCACAACCT GAAGCTACCA 
TCTGTCAATC CCGATTCGGA TCTTCGTGAC TTTCGCCTGT GCATGGAGCA CTTTGAGCCG 
CATCAAATCG AGAACGGAGC CCCAGTGAAG ATGGCCGTTC CGACCCTCAA GCTTGGGCAC 
TCCAGTCCGA ATATCTTTAA GAACAGCGAG AGCACGCTGC CGGGATGCCT GTGGCCCTCG 
TGTCCGCCCA ATCGCAAGAT CTGCTACGAT CTGCCCGACA ATGAGGCCGT TCGAGCGGCC 
TGGCTGTCGT ATGTGCGGCT GCCGCCGGAC AGCCAGGGTC GTCTGTGTGG TCTGCACTTT 
CTGCAGCTGT ACGAGGAGGT GGATCTGCCA GGAGATGTAC CCGAAGCGGT GCTCGAGCGA 
CTGCAGGGTA TCTATGATCA GGCCTCCATC TCGCTGAAGT TTCAGTGCTC GGTGCAGGGA 
TGTGGCTCCA AGTACAAGCA GGATACGCAT TTGACAAAGC TGCCACGGGA CGCGGAACTG 
CTGGCCAAGT GGCTGCACAA CACCAGGATC TCCTACGATC GCTCCTTGCA TTTTAGCTAC 
CGCATTTGTC TATTGCACTT TGAGGCGTTC TGCTTGAATG GCGTGCGCCC GCAGACCTGG 
GCCATACCTA CGCTCCAGCT AAATCACGAC GGAGAGATCT ACCAAAATAC CGCCAAGCAG 
GAGATCCCAG AGATGCCCCT GAAGCAGGAG ATCCTCGAGA ATCCCGTGAA GCAGGAGAAA 
TCGCACTGTG GGAGAATCTC CAGCCTGAGT CTCTCCATCC CCCTGCACAT CAAGACGGAA 
CAGGGTCCTG TCCTGCGACC TCGAGGCACT TGGGGCACAT CTTCTCAGAG CAGTCCCTGC 
CTGAGCGCCA GCTCCAGTCC CCGCCTGAAA AACAGAATCT GCTGCGTCTC CGATTGCGGA 
GAGTACGCCA AATCCCAGCG GCTGTACCGC TTTCCCACCG CCGAACCGGC ACTGCTCAAG 
TGGCTGGTTA ATACCCAGCA AAAACCGGGA CTCGTGGACA TCCAGAACCT GTTTGTGTGC 
CAGCTTCATT TCGAGGCGGA CGCCATTAAC CAAACGCAGC TCAGGAGCTG GGCCGTGCCC 
ACACTGAGGC TGGGCCACGA TGGGCATGTC ATACCAAATG CCAGACACAA TGGGAACATA 
GCCAACAGCC AGGAAACGGA GCAGGCCATG GAGTTCATTC GGGCCAACTA CTGCGCCGTG 
TTGAGCTGCT TCCAGCCGAA AGGAGATGGT GTACGCTTCT ACAAGTATCC CAGCGACATT 
GCCATGGCGC GCAGGTGGGC CACGAATCTC AAGCATCGCT CCATGCAGGC CAGCAGTCAT 
GGCTTCCTGG TTTGCCAGTC CCACTTTGCA GCCGACTGTT TTGATCCGGA GACGGGAGAC 
CTGCGCGAGG ACGCCGTACC CGTGGCCACA CTCGCAGAGA ACGTGAAATC AGAGGGCCTG 
CTGCTCCGTT GTCTGGTAAG GGGTTGCTCT ACGGATAACT CTGAAAAAGG ACTGCTGTTC 
AAGGTGCCAA AAAAGAATCG TGTACGGGAT GTGTGGGCCC ACAATCTGTG GATGCATCCG 
ATAGAGCTGA TGGGCGAGCA CTACATCTGC GATCGACATT TCGAGGCGCA TTGCGTGAAT 
GAACACAAAT TACTCCACGC GGGCTCAGTG CCAACCCTCC ACCTGGGACA CAACGAACCG 
CTGGAACTAC TGCCCAATCC CCAGACCTTC CAGGACTTCC CTGAGGAGTG CGAGTGCTGT 
GTGCCCGGCT GTGGACGCAC CAATCGGAAG GAGGAGGATC TGCAGTTTAG CAAATTTCCC 
AAGTGGCGAG TCCTGTATGA AAAGTGGCTG CACAACTTCC GCCTCGAAGT GCCCAAGGAG 
CAGCGCATCG GGACGCTGAG AGTGTGTCAC ATGCACTTTG AGGAGAGCTG CTACGATGGC 
CAGAACGTGC GCAGGGGAGC TATGCCCACC CTAGAGCTGG GACACTCGCA TCCAGACATT 
TATCGCTCCG ACAAGGGATC GCTGTGGAAG AAGGTTCACA AGAGACTCAC CGATTGTTGC 
TATCCCGATT GCTACGAGGA GTGTCACAAG GCCAACACGA ATCGCACGAC CTACGATCTG 
CCCAGCGATG GGCCACTGCG CGAGTCCTGG CAGCAGCACA TGGCCATCCC TGCCAGCGGT 
GAGGATGGCT CCTCAGTGCT AAAGCTCTGT GCCCTACACT ACATCATTAT GTACGAGCAC 
AGCGAACAGA GCTTCCCAGA ACACGGACCG AATCTACTGC TGGACAAGAA CTACGAGCAC 
GCCCGTCAGT TGGCGTATCT GCGGCGCTTC TTGTGTGCGG TACAGGGCTG TCGTTATTTG 
CAGCCGCGGG ATGGGGGTCC GATGCACGGA ATACCCCGGC GGAGGGAGAT CCTTCGGATG 
TGGGTGGAGA ATGCCCAGCT GCGGCTGAAC GAGCACGAAA TTTACATGAC GAAGCTGTGC 
AGCAAACATT TTGAGGCCCA CTGCCTGTTC GAGGGTAAAA AATGCTATCC CTGGAGCGTG 
CCAACGCTCC ATCTGCCAGA GGCGCAGCCC GGGCAGGTGC TCCACCAGAA TCCCACCAAG 
GAGGAGTGGC AGGAAATGAA ACAGAGAATG AAATTGGAAG AGCAGACGCC GAAGACGGAA 
GAGCAGGCAG ATGGACTACT AATGGAACCC TATGTGAAGA TGGAACCCCA CGACGACGAG 
TCACAAACGG AGTCGGAATT GCTGATAAAT GAGAGCACAC TGGACTCTCA AGAACTCTCT 
CAAGACTTTC CAACACAAGA TCCAATCGAA ATGCCCGCCC TGGAGGTGCT CCTAGAGGTG 
AGCCATGTCG AGAAGCTGGA TAGCTACGAG AGGAATGAAT ACCCTGCGGA TACCTCTGCC 
AACACAAATT CTCCGAACAA ACGTTTCCGC CATCAGTACA GTGCCCACAA GTGTAGTGTC 
GAGGGATGTC GCGTGTCGCT CGAGGACCTT GATGGGAATC TGAAGCTGCA CAAGCTACCC 
AGCTCCACGG AGGCGGCCGG GAAGTGGCTG TACAACATTC ACGTGGAGAT AGAGGATAAA 
TGGCGGATAC GCGTCTGCAG CCATCACTTC GACAGGCAGT GCCTCAATGG TTCAAGGCTC 
AGGAGGGGAT CGATGCCCAC TCTGCTGCTG GGGCCGCGTG TTCCAGAGAC TATCCATCAG 
AATGAGTTTG CGCAGCTACA ATTGGACGAT GCACCAGCCC ATAATGGCAA TACATTGGAG 
CGATCCATTG GAAAGGTTGC GCAGATATGC GTTCCACGTC CGTCGCCGCC GCGTAAGTCG 
AGCAAATTCT GCCAGATCGA GGGATGTTCG AACCATCTGA CCAGCGAGAA TATGACGCTA 
CACAAGTTCC CGCACTCGTC GTGGATCTGC ACCAAATGGC AGCACAACAC ACAGGTGCCG 
TTCGATCCGG AGTACCGCTG GCGCTATCGC ATCTGCAGCG CCCACTTCCA TCCCGTGTGT 
ATGGTCAATA TGCGGCTGCT GCATGGCAGT GTGCCCACCC TGAAGCTTGG TCCACGGGCA 
CCCGGCGAAC TCTTTGACAG CGACTTTGAG GCCATAAACA TAAAGATTGA AAAAATGGAG 
AAGATGGAGA GGAAATCTGA GGCTCTGAGA AGCACCACTG GAGATAGATA TCCCACCATG 
CAGGCCATGG GGGAGAAGAA GTTCAAGACT GAGGAGCTGG AAGATGGAAT GGAGGAGGAG 
GATGACATGC TCTGCCTGGA GCCAGAGATG CAGCTATACG AAGATCAGGA AGAACAGCAA 
CAGAAGCCAA AGATAAATCT TGGAGTCCCC AATGGCGGCT GGAAAACGGA ACTCCGTTTG 
CCATCGAAGG GTAGGGTGGC GTTCAATCCG GTGAGATCTG GCTACGACAA GTGCTCGCTG 
ATGCATTGCC AGCGCCAGAG ATCGAAGCAC GGCGTCCACA TCTACAAGTT CCCCCGATCG 
CAGGAGCACC AACAGCGATG GATGCACAAT CTGCGCATAC GCTACGATGA GAAGCGACCC 
TGGAAGTTTA TGGTCTGCAG CGTGCACTTT GAACCGCATT GCATACGGCT GCGGAAGCTG 
CGGCCCTGGG CAGTTCCCAC GCTAGAGCTG GGAGACAATG TGCCCGAGGA CATCTATACG 
AACGAGCAGT GCCAGATGTT TGCCAGTGGA CAGGGAGGAG AGATCAATGG CATCGATAGC 
GATGAGGTGG AGGCGGAGCC GGAGGCAGAA GGGGAGAGCG ATGGGAATGA TGAGGATGGC 
CTGCAGGAGG ACGAGGATGA GGAGACAGAC GACCAGGAGC CCATCGCCAA GAAGCGTCGT 
CGCTCGCGGC TGGATGCCGT CTGGCCTCCC GGCCAGGCGC CACCATGGAA GGTGAAACAA 
TGCTGTCTTC CCTACTGTCG CAGTCCACGC GGCGAGGGCA TCAAGCTGTT TCGACTGCCC 
AACAAAGTCA ACTCCATCCG CAACTGGGAG CTGGCCACGG GCATGAAGTT CAAGGAGTCG 
CAGCGCAACA CGAGACTCAT CTGCAGCCGC CACTTTGATC CGGAGCTGAT CGGAGTGCGT 
CGTCTGATGC GCAATGCCAT TCCCACCAGG CATCTGGGAC CCACGGGCGA TGTAAAGCCA 
GTGATGGCTC CACCGACAGC TGGTCCCAAA TGCTGTATGG CAGATTGTAC CTATGATGTG 
GCGGATGTGA AGCTGCACAA GTTTCCCAGC AATCCCAAAC TCCTGAAGGA GTGGTGCCAG 
GCATTAAGGG TCACGGATAT GCAAAGGTAT CGCGGCAAGC ACATTTGCTC CGCCCATCTA 
CCTGTCCACG AGGCCGTACA GTGCATTGTT TGTGGCGCGG AGAAAGCACC CCTGCTGCCG 
ATGCTTAATT TTCCCGCTAA CCGGAATCAG CGCGCCAAAT GGTGCTACAA TCTTAAGATC 
GAAACGATAC CCAAGTGGGA CATATCCAAG CACATTTGCT GTAAACACTT TGAGCCATAT 
TGTTTTGCAG AGGCGGGTCT CCTAAAGCCA GAGGCGGCAC CCACGCTGCA TTTGAATCAC 
AATGATACAA ATATATTCCT TAACGATTGT GCCATAAATC CTGCCTACAG CGTATGCGTG 
AAGGATGAAC CCATGGACAA TCAGGTCCTG TCATTGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA10453 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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