Dpse\GA22026 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA22026
Entrez Gene ID	4815877
Full Name	GA22026 gene product from transcript GA22026-RB
Synonyms	GA22026,dpse_GLEANR_11463
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001355471.4	XP_001355507.4	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA22026 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA22026 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe23811	XM_001355471.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA22026), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA22026 ( XM_001355471.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355507.4 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA22026), partial mRNA.

Price & Availability↑

CloneID	ODe23811
Clone ID Related Accession (Same CDS sequence)	XM_001355471.4
Accession Version	XM_001355471.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589961). On Dec 22, 2015 this sequence version replaced XM_001355471.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTGGCCAT GGCGAATGCC GGCCGCACTC CATTTGAGCG AGCTGGAACG AGCGAACACG 
CTCGAGCGCG AGATGCGCGA TCCGACCAGC ATTTGCAATG TGGACTGCCT GCTGGATACG 
GTATCGGCCC TGGTCAGCGA CTGCGACCAC GACACCCTCA AGCGGCTGAA AAACATCGAA 
CAGTATGCTG CCAAATATAA ACCCCTGGCG CAGAGGATCA ATCAGCTGCG CATGAATGTC 
GAGGACTTCG ACTTCATTAA GCTAATCGGC GCCGGGGCCT TTGGAGAGGT GCAACTGGTG 
CGGCACAAGT CCTCCAGCCA GGTGTACGCC ATGAAGCGGC TGTCCAAATT TGAGATGATG 
AAGCGCCCGG ACTCGGCCTT CTTCTGGGAG GAGCGTCACA TTATGGCCCA TGCCAACTCC 
GAGTGGATTG TCCAACTGCA TTTTGCATTT CAGGACTCCA AATACCTGTA CATGGTGATG 
GATTTTATGC CCGGCGGTGA CATCGTCTCG CTGATGGGGG ACTACGATAT ACCCGAGAAG 
TGGGCCATTT TCTACACAAT GGAGGTAGTG CTGGCCCTGG ACACCATTCA CAACATGGGC 
TTTGTGCACC GTGACGTGAA GCCGGACAAC ATGCTGCTCG ACAATTACGG GCATCTGAAG 
CTCGCCGATT TCGGCACCTG TATGCGCATG GGCGCCAACG GCCAGGTGGT GTCCAGCAAT 
GCGGTCGGCA CGCCGGACTA CATCAGTCCG GAGGTGCTGC AGTCGCAGGG TGTCGACAAT 
GAGTATGGTA GGGAGTGCGA CTGGTGGTCG GTGGGGATCT TCCTCTACGA GATGCTCTTC 
GGGGAGACGC CCTTCTACGC GGACTCACTG GTGGGCACCT ACGGCAAGAT CATGGACCAC 
AAGAACTCGC TCAGCTTCCC CCAGGAGGTG GAGATCAGCG AACAGGCCAA GGCCCTGATC 
CGGGCATTCC TCACAGATCG GACGCAGCGT CTGGGCCGCT ACGGCATCGA TGATATCAAG 
GCGCATCCGT TCTTCAGAAA CGACACCTGG TCCTTTGACA ATATCAGGGA GAGTGTGCCG 
CCGGTTGTGC CGGAGCTAAC CTCGGACGAC GATACGCGCA ACTTTGAGGA CATCGAGAGG 
GACGAAAAGC CCGAGGAGGT GTTCCCCGTA CCGAAGGGCT TTGATGGCAA TCACCTGCCC 
TTCATCGGCT TCACCTACAC GGGTGACTAC CAGCTGCTGT CCAGCGACAC CGTCGACGCC 
GAGGCCAAGG AGCCCAATTC GGTGAACAGC AACACTAGCA ACAACCATGC CCACGGCCAT 
GGGCACAACC ATCGCCATCG TACCTCCAAC TCCAACGAGC TGAAGCGGCT CGAGGCACTG 
CTCGAGAGGG AGCGCGGCCG AGCCGATGCT CTGGAGAAGC AGGAAACCAG CCTGCGGCAC 
AAGATCGAGC TGAGCGGCAA GCGTGAGGTC GAACTGCAGC GCATTGCGGC CGAGTACGAG 
AAGGATCTGG CGCAGCGAAA GAACAACTAC AAGGTGGCCA TGCAGAAGGT GGAGCAGGAG 
ATCGAGCTGC GCAAGAAGAC GGAGGCCCTG CTGGTCGAGA CGCAGCGCAA TCTGGAGAAC 
GAGCAGAAGA CGCGCACACG CGATCTCAAC ATCAGCGAGA AGGTCGTCTC GCTGGAGAAG 
CAGCTGCAGG AGATGGAGCA CAGCTACAAG AGCGAGACGG AGAACACGCA GAAGCTGAAG 
AAGCAGAACG CGGAGCTCGG CTTCACACTG AAGACGCAGG AGGAGAAGGT GCGCGACATG 
GTCGACATGA TCGACACACT GCAGAAGCAC AAGGAGGAGC TGGGCCAGGA GAATGCCGAT 
CTGCAGGCGC AGGTGGTGCA GGAGAAGAAC ATGCGCTCCC AGCTGAAGGA ACTGCATAAG 
GAGACGGAGA ACAAGATGCA GACCCTCACC AATGACATTG AGCGCACCTT GGTCCGCGAG 
CAGAAGGCCA AAGAGGATAA CCGCGCGCTG CTGGAGAGGA TCAGTGAGCT GGAGAAGAGC 
CATGCAAGTC TCGACTTTGA GCTGAAGGCG GCCCAGGGTC GCTACCAGCA GGAGGTCAAG 
GCCCACCAAG AGACTGAAAA GTCGCGACTG GTCTCTCGCG AGGAGGCCAA TCTGCAGGAG 
GTCAAGGCCC TGCAGTCCAA GCTGAACGAG GAGAAGTCCG CCCGGATCAA GGCGGACCAG 
AACTGGCAGG AGAAGGAGCG CCAGCTGAAC ATGCTCTCCG TGGACTACCG CCAGATCCAG 
CTGCGGCTGC AGAAGCTGGA GGGCGAGTGC CGCCAGGAGT CCGAGAAGGT GGCCGCCCTC 
CAGTCGCAGC TGGACCAGGA GCACAGCAAA CGCAACGCCC TGCTCTCGGA GCTCAGTCTG 
CTCAGCTCGG AGGTGGCGCA TCTGCGCTCC CGCGAGAATC AGCTGCAGAA GGAGCTCTCC 
ACGCAGCGCG AGGCCAAGCG GCGCTTCGAG GAGGACCTGT CGCAGCTGAA GGGCACCCAC 
CACGAGGCCC TGGCCAACAA TCGGGAGCTG CAGGAGCAGC TGGATGCCGA GCTGTGCTTC 
TCCCGGCTGT ACAAGACGCA GGCCAACGAG AATCGGGAGG AGAGCGCCGA GCGTTTGGCC 
AAGATCGAGG ATCTGGAGGA GGAACGCGTC TCGCTCAAGC ACCAAGTGCA AGTGGCCGTC 
GCTCGGGCCG ACTCGGAGGC CCTGGCCCGC TCGATAGCCG AGGAAACGGT GGCCGATCTC 
GAGAAGGAGA AGACCATCAA GGAGCTGGAA CTGAAGGACT TCATCATGAA GCACCGCAAC 
GAGATCAATG CCAAGGAGGC GACGCTCGCC ACCCTCAAGG AGGCGGAGAC AGAGCTGCAC 
AAGAAGCTCG GCCAGAAGGC CAACGAGTAC GAGGATCTCG TCCAGCAGCA CAAGAAGCAG 
CAGGACGAGC TGGGCCAGCT GCGCGCCACC AAGGACGAGG AGATCACCAA GCTGCTGGAT 
AAATGCAAGA CCGAGGTGTT GCTCAAGCAG GTGGCCGTCA ACAAGCTGGC CGAGGTGATG 
AACCGACGCG ACTCGGACTT GCAGAAGCAA AAGGGCAAGG CCCGGTCCAC GGCCGAGCTG 
CGCAAGAAGG AGAAGGAGAT GCGCCGCCTG CAGCAGGAGC TGTCCCAGGA GCGTGACAAA 
TTCAATCAGC TGCTGCTGAA GCACCAGGAC CTGCAGCAGC TGTGCGCCGA GGAGCAGCAG 
CACAAGCAGA AGATGGTCAT GGAGATCGAC TGCAAGGCCA CCGAGATTGA GCATCTCCAG 
AGCAAGCTGA ACGAGACGGC CTCGCTCTCA TCCGCCGACA ACGATCCCGA GGACAGTCAG 
GACTCGGTCT TCGAGGGCTG GCTGAGTGTG CCAAACAAGC AGAACCGTCG GCGCGGCCAC 
GGCTGGAAGC GCCAGTATGT GATTGTCTCC TCGCGCAAGA TCATCTTCTA CAACTCGGAC 
ATCGACAAGC ACAACACGAC GGACGCTGTG CTCATACTGG ATTTGAGCAA GGTATACCAC 
GTGCGCAGCG TCACCCAGGG CGATGTCATA CGCGCCGATG CCAAAGAGAT TCCGCGCATC 
TTTCAGCTGC TGTATGCCGG CGAGGGCGCC TCTCATCGTC CGGACGAGCA GAGCCAGCTG 
GATGTGAGCG TGCTGCATGG CAACAGCAAC GAGGAGCGTC CCGGCACCAT CATCCACAAG 
GGTCATGAAT TTGTGCACAT CACCTACCAC ATGCCCACAG CGTGCGAGGT GTGCCCGAAG 
CCATTGTGGC ACATGTTCAA GCCGCCGGCG GCTTACGAAT GCAAGAGATG CCGCAACAAG 
ATACACAAGG AGCATGTGGA CAAGCATGAT CCGTTGGCGC CCTGCAAGCT CAATCATGAT 
CCGCGCTGTG CCCGCGATAT GCTGCTGCTG GCCTCCACGC CCGAGGAGCA ATCGCTGTGG 
GTGGCCCGTC TGCTGAAGCG TATCCAAAAG AGTGGATACA AGGCGAATTC GTCAAACAAC 
AACAGCACCG ATGGCAGCAA GATATCGCCC AGCCAATCGA CCCGCTCCTC CTACAAGCCG 
TTTGTTGCGA ACGTGCAACG CTCCGCCACG CTACCAGCCC TCCCTTTCTC TCTCTTAAGT 
CCCAAGGCGC GACGTGCTAT AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355507.4
CDS	1..4167
Translation	MWPWRMPAALHLSELERANTLEREMRDPTSICNVDCLLDTVSALVSDCDHDTLKRLKNIEQYAAKYKPLAQRINQLRMNVEDFDFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDSKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDNYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPQEVEISEQAKALIRAFLTDRTQRLGRYGIDDIKAHPFFRNDTWSFDNIRESVPPVVPELTSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTYTGDYQLLSSDTVDAEAKEPNSVNSNTSNNHAHGHGHNHRHRTSNSNELKRLEALLERERGRADALEKQETSLRHKIELSGKREVELQRIAAEYEKDLAQRKNNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRTRDLNISEKVVSLEKQLQEMEHSYKSETENTQKLKKQNAELGFTLKTQEEKVRDMVDMIDTLQKHKEELGQENADLQAQVVQEKNMRSQLKELHKETENKMQTLTNDIERTLVREQKAKEDNRALLERISELEKSHASLDFELKAAQGRYQQEVKAHQETEKSRLVSREEANLQEVKALQSKLNEEKSARIKADQNWQEKERQLNMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSELSLLSSEVAHLRSRENQLQKELSTQREAKRRFEEDLSQLKGTHHEALANNRELQEQLDAELCFSRLYKTQANENREESAERLAKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFIMKHRNEINAKEATLATLKEAETELHKKLGQKANEYEDLVQQHKKQQDELGQLRATKDEEITKLLDKCKTEVLLKQVAVNKLAEVMNRRDSDLQKQKGKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQHKQKMVMEIDCKATEIEHLQSKLNETASLSSADNDPEDSQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNSNEERPGTIIHKGHEFVHITYHMPTACEVCPKPLWHMFKPPAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRCARDMLLLASTPEEQSLWVARLLKRIQKSGYKANSSNNNSTDGSKISPSQSTRSSYKPFVANVQRSATLPALPFSLLSPKARRAID

Target ORF information:

RefSeq Version	XM_001355471.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA22026), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355471.4

ORF Insert Sequence:

ATGTGGCCAT GGCGAATGCC GGCCGCACTC CATTTGAGCG AGCTGGAACG AGCGAACACG 
CTCGAGCGCG AGATGCGCGA TCCGACCAGC ATTTGCAATG TGGACTGCCT GCTGGATACG 
GTATCGGCCC TGGTCAGCGA CTGCGACCAC GACACCCTCA AGCGGCTGAA AAACATCGAA 
CAGTATGCTG CCAAATATAA ACCCCTGGCG CAGAGGATCA ATCAGCTGCG CATGAATGTC 
GAGGACTTCG ACTTCATTAA GCTAATCGGC GCCGGGGCCT TTGGAGAGGT GCAACTGGTG 
CGGCACAAGT CCTCCAGCCA GGTGTACGCC ATGAAGCGGC TGTCCAAATT TGAGATGATG 
AAGCGCCCGG ACTCGGCCTT CTTCTGGGAG GAGCGTCACA TTATGGCCCA TGCCAACTCC 
GAGTGGATTG TCCAACTGCA TTTTGCATTT CAGGACTCCA AATACCTGTA CATGGTGATG 
GATTTTATGC CCGGCGGTGA CATCGTCTCG CTGATGGGGG ACTACGATAT ACCCGAGAAG 
TGGGCCATTT TCTACACAAT GGAGGTAGTG CTGGCCCTGG ACACCATTCA CAACATGGGC 
TTTGTGCACC GTGACGTGAA GCCGGACAAC ATGCTGCTCG ACAATTACGG GCATCTGAAG 
CTCGCCGATT TCGGCACCTG TATGCGCATG GGCGCCAACG GCCAGGTGGT GTCCAGCAAT 
GCGGTCGGCA CGCCGGACTA CATCAGTCCG GAGGTGCTGC AGTCGCAGGG TGTCGACAAT 
GAGTATGGTA GGGAGTGCGA CTGGTGGTCG GTGGGGATCT TCCTCTACGA GATGCTCTTC 
GGGGAGACGC CCTTCTACGC GGACTCACTG GTGGGCACCT ACGGCAAGAT CATGGACCAC 
AAGAACTCGC TCAGCTTCCC CCAGGAGGTG GAGATCAGCG AACAGGCCAA GGCCCTGATC 
CGGGCATTCC TCACAGATCG GACGCAGCGT CTGGGCCGCT ACGGCATCGA TGATATCAAG 
GCGCATCCGT TCTTCAGAAA CGACACCTGG TCCTTTGACA ATATCAGGGA GAGTGTGCCG 
CCGGTTGTGC CGGAGCTAAC CTCGGACGAC GATACGCGCA ACTTTGAGGA CATCGAGAGG 
GACGAAAAGC CCGAGGAGGT GTTCCCCGTA CCGAAGGGCT TTGATGGCAA TCACCTGCCC 
TTCATCGGCT TCACCTACAC GGGTGACTAC CAGCTGCTGT CCAGCGACAC CGTCGACGCC 
GAGGCCAAGG AGCCCAATTC GGTGAACAGC AACACTAGCA ACAACCATGC CCACGGCCAT 
GGGCACAACC ATCGCCATCG TACCTCCAAC TCCAACGAGC TGAAGCGGCT CGAGGCACTG 
CTCGAGAGGG AGCGCGGCCG AGCCGATGCT CTGGAGAAGC AGGAAACCAG CCTGCGGCAC 
AAGATCGAGC TGAGCGGCAA GCGTGAGGTC GAACTGCAGC GCATTGCGGC CGAGTACGAG 
AAGGATCTGG CGCAGCGAAA GAACAACTAC AAGGTGGCCA TGCAGAAGGT GGAGCAGGAG 
ATCGAGCTGC GCAAGAAGAC GGAGGCCCTG CTGGTCGAGA CGCAGCGCAA TCTGGAGAAC 
GAGCAGAAGA CGCGCACACG CGATCTCAAC ATCAGCGAGA AGGTCGTCTC GCTGGAGAAG 
CAGCTGCAGG AGATGGAGCA CAGCTACAAG AGCGAGACGG AGAACACGCA GAAGCTGAAG 
AAGCAGAACG CGGAGCTCGG CTTCACACTG AAGACGCAGG AGGAGAAGGT GCGCGACATG 
GTCGACATGA TCGACACACT GCAGAAGCAC AAGGAGGAGC TGGGCCAGGA GAATGCCGAT 
CTGCAGGCGC AGGTGGTGCA GGAGAAGAAC ATGCGCTCCC AGCTGAAGGA ACTGCATAAG 
GAGACGGAGA ACAAGATGCA GACCCTCACC AATGACATTG AGCGCACCTT GGTCCGCGAG 
CAGAAGGCCA AAGAGGATAA CCGCGCGCTG CTGGAGAGGA TCAGTGAGCT GGAGAAGAGC 
CATGCAAGTC TCGACTTTGA GCTGAAGGCG GCCCAGGGTC GCTACCAGCA GGAGGTCAAG 
GCCCACCAAG AGACTGAAAA GTCGCGACTG GTCTCTCGCG AGGAGGCCAA TCTGCAGGAG 
GTCAAGGCCC TGCAGTCCAA GCTGAACGAG GAGAAGTCCG CCCGGATCAA GGCGGACCAG 
AACTGGCAGG AGAAGGAGCG CCAGCTGAAC ATGCTCTCCG TGGACTACCG CCAGATCCAG 
CTGCGGCTGC AGAAGCTGGA GGGCGAGTGC CGCCAGGAGT CCGAGAAGGT GGCCGCCCTC 
CAGTCGCAGC TGGACCAGGA GCACAGCAAA CGCAACGCCC TGCTCTCGGA GCTCAGTCTG 
CTCAGCTCGG AGGTGGCGCA TCTGCGCTCC CGCGAGAATC AGCTGCAGAA GGAGCTCTCC 
ACGCAGCGCG AGGCCAAGCG GCGCTTCGAG GAGGACCTGT CGCAGCTGAA GGGCACCCAC 
CACGAGGCCC TGGCCAACAA TCGGGAGCTG CAGGAGCAGC TGGATGCCGA GCTGTGCTTC 
TCCCGGCTGT ACAAGACGCA GGCCAACGAG AATCGGGAGG AGAGCGCCGA GCGTTTGGCC 
AAGATCGAGG ATCTGGAGGA GGAACGCGTC TCGCTCAAGC ACCAAGTGCA AGTGGCCGTC 
GCTCGGGCCG ACTCGGAGGC CCTGGCCCGC TCGATAGCCG AGGAAACGGT GGCCGATCTC 
GAGAAGGAGA AGACCATCAA GGAGCTGGAA CTGAAGGACT TCATCATGAA GCACCGCAAC 
GAGATCAATG CCAAGGAGGC GACGCTCGCC ACCCTCAAGG AGGCGGAGAC AGAGCTGCAC 
AAGAAGCTCG GCCAGAAGGC CAACGAGTAC GAGGATCTCG TCCAGCAGCA CAAGAAGCAG 
CAGGACGAGC TGGGCCAGCT GCGCGCCACC AAGGACGAGG AGATCACCAA GCTGCTGGAT 
AAATGCAAGA CCGAGGTGTT GCTCAAGCAG GTGGCCGTCA ACAAGCTGGC CGAGGTGATG 
AACCGACGCG ACTCGGACTT GCAGAAGCAA AAGGGCAAGG CCCGGTCCAC GGCCGAGCTG 
CGCAAGAAGG AGAAGGAGAT GCGCCGCCTG CAGCAGGAGC TGTCCCAGGA GCGTGACAAA 
TTCAATCAGC TGCTGCTGAA GCACCAGGAC CTGCAGCAGC TGTGCGCCGA GGAGCAGCAG 
CACAAGCAGA AGATGGTCAT GGAGATCGAC TGCAAGGCCA CCGAGATTGA GCATCTCCAG 
AGCAAGCTGA ACGAGACGGC CTCGCTCTCA TCCGCCGACA ACGATCCCGA GGACAGTCAG 
GACTCGGTCT TCGAGGGCTG GCTGAGTGTG CCAAACAAGC AGAACCGTCG GCGCGGCCAC 
GGCTGGAAGC GCCAGTATGT GATTGTCTCC TCGCGCAAGA TCATCTTCTA CAACTCGGAC 
ATCGACAAGC ACAACACGAC GGACGCTGTG CTCATACTGG ATTTGAGCAA GGTATACCAC 
GTGCGCAGCG TCACCCAGGG CGATGTCATA CGCGCCGATG CCAAAGAGAT TCCGCGCATC 
TTTCAGCTGC TGTATGCCGG CGAGGGCGCC TCTCATCGTC CGGACGAGCA GAGCCAGCTG 
GATGTGAGCG TGCTGCATGG CAACAGCAAC GAGGAGCGTC CCGGCACCAT CATCCACAAG 
GGTCATGAAT TTGTGCACAT CACCTACCAC ATGCCCACAG CGTGCGAGGT GTGCCCGAAG 
CCATTGTGGC ACATGTTCAA GCCGCCGGCG GCTTACGAAT GCAAGAGATG CCGCAACAAG 
ATACACAAGG AGCATGTGGA CAAGCATGAT CCGTTGGCGC CCTGCAAGCT CAATCATGAT 
CCGCGCTGTG CCCGCGATAT GCTGCTGCTG GCCTCCACGC CCGAGGAGCA ATCGCTGTGG 
GTGGCCCGTC TGCTGAAGCG TATCCAAAAG AGTGGATACA AGGCGAATTC GTCAAACAAC 
AACAGCACCG ATGGCAGCAA GATATCGCCC AGCCAATCGA CCCGCTCCTC CTACAAGCCG 
TTTGTTGCGA ACGTGCAACG CTCCGCCACG CTACCAGCCC TCCCTTTCTC TCTCTTAAGT 
CCCAAGGCGC GACGTGCTAT AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA22026 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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