Dpse\GA14656 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA14656
Entrez Gene ID	4814852
Full Name	GA14656 gene product from transcript GA14656-RC
Synonyms	GA14656,dpse_GLEANR_691
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354486.3	XP_001354522.3	uncharacterized protein, isoform C
XM_015185947.1	XP_015041433.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA14656 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA14656 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe24288	XM_001354486.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA14656), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe24288	XM_015185947.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14656), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA14656 ( XM_001354486.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354522.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA14656), mRNA.

Price & Availability↑

CloneID	ODe24288
Clone ID Related Accession (Same CDS sequence)	XM_001354486.3 , XM_015185947.1
Accession Version	XM_001354486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). On Dec 22, 2015 this sequence version replaced XM_001354486.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAGTA CCAATCTGGT GGTGCCGGTG GTGCTGTGGG GACCCACGGC GCCCACGCAC 
TGCATCTCCA GTGTGTTTCT TTCGGACGAC CAGTGCACCC TGGTCACCGG CTGCTACGAC 
GGACAGATTT GTCTCTGGCA GGTGGAGCCC AGCACACTGA AGATGTCGCC CCGCTGCCTG 
CTGGTGGGCC ACTCGGCTCC GGTGCTCTGC CTGGTGCGGG CCTCGCTGCT GCCCGAAAAC 
AACTTCCTGG TGAGCTCCTC GGAGAACGGC GAGATGTGCA CCTGGGACCT CATCGACGGC 
AAGTGCATGG AGGCAGTCAA GCTGCCGCAG GTGCACACAC AGATCCAGAG CTATCACACG 
GCCAATAGCG AGGACATTCG TCTGTTCTGC ATCGGCTATT ACGCCGAGAT CATGGTGATG 
GACCCGTTCA GCCTGGAAGT AATCTACGTG CTCAGCTCCA AGGTGAAGCC AGACTGGATC 
TCGGCCATCC ACGTGCTCCG GCCCATGCGG CGCAAGGACG ACGTGGTCCT GGCCATCACC 
ACCACGGGCA CAGTGAAGGT GTGGACTCTG ACGGGGAACG AGAACAAGCA CGCGGAGCCC 
ATCTACGAGA ACGAGTCGAA GGAGATCCGC TGCCTCAACG CCATAACCAT GAACTGCTGT 
GCCCAGAACC AGCGCACTGT GCTCTTGGTG TGCACAAAGT ACTGGCAGAT CTACGACGCC 
GGGGACTTTA CCGTTCTCTG CTCCGTGATC GCGCCGGCCC GCGAGCGCTG GCAGGGCGGC 
GACTTCATCA CATCTGACCG CGTCATGCTC TGGACGGACG AGGGCAAGGG CTACCTGTAC 
AGGCTGCCCG CCAACTGCAT CCCCGACAAC AAGGAGTTCC ACTCGAAGAG CGTCGTCCGG 
GACGCCCCCT ACCTGTACTA CGTGCTCCAG CATGCCGGCG ACAAGGTACT CTCCTGTCCA 
CCAGCCATGA AACTGCTGCT GGCCGGAAGC AACGCAATAG GCAATGGAAA TGGCAACGGC 
AGTGGCGGAC AGCACAACCT GCTGCGCGGC GACTCTGAGG GGTACATCTC GGTGTGGAAT 
GTGCCGGAGG TGCCGCTGGA CAACATCAGC ATACTGCAGG CCAAGCAGAT GCCGCCGCGC 
GTCCTCAAGC CACACGTGTG CACTTCTCTG GTAGAGGCCT GGTCCATTAT GGATCCGCCG 
CCCGTGGGGA TACTGGATCA GCTGTCGAGG ATCACCGAGT CCCCGGTTAA ACTCACCTCG 
AGCATATACT TGCCGCAACA GAGTCGGCTG GTCATCGGGC GCGAGGATGG CAGCATTGTG 
ATCGTGCCCG CCACCCAGAC GGTGATGATG CAGTTGCTGG TTGGCATCAA GCAGAACTTC 
AGCGACTGGC CGTCGCACCA GATCTTGTAC GGGCACCGGG GCAGGGTGAA CTGCCTGCTC 
TGTCCCTCCA TGATCCACTC GCGGTACGAG AAGTCGCACC TGCTGTCCGG CGGCATCGAC 
TTTGCCGTCT GCCTCTGGGA CCTGTACAGC GGCAGCATGC TGCACCGCTT CTGCGTGCAC 
GCGGGGGAGA TCACCCAGCT GCTTGTGCCG CCGGAAAGCT GCAGCCCGCG GATCCTCAAG 
TGCATCTGCT CGGTCGCCTC GGACCACTCG GTGACTCTTG TCTCCCTGCA GGAGCGCAAG 
TGCGTTACCC TGGCCAGTCG CCACCTGTTC CCCGTGGTGA CCATCAAGTG GCGTCCACTG 
GACGACTTCC TCATCGTGGG CTGCTCGGAC GGCTCCGTCT ACGTGTGGCA GATGGAAACC 
GGCCACCTGG ACCGGGTGCT CCACGGAATG CTGGCCGAAG AGGTGCTCTC CGCCTGCGAC 
GAGCAGGCTG GCGAGGACGG GGTCGGTGGT GGCTCCTCGG GGGTGAGCGG CGCCTCGACC 
AGCGAAATGG GGATGGCCAA TCCGGCGGTT CACTTCTTCC GCGGCCTCAA GTCGAGGAAC 
ATGAACGCCA TCCGCCACGC CACCCAGCGC GGGATCACCC AGCTCCAGCA GCTGCAGGGC 
CACAACCAAG GGAACTTTGA CTTCCTCATG AAGCACCGCA GCAATCCACT GGTGATCCAG 
GGACTGCGCA CCAACCCCAA GGACGCCGAG AGCCACATCC TGTTCTTCGA CATCGAGGGA 
CTGATCTTCG AGCTGCACAG CGAGGAGTAC GCCCAGATGA CGCCGGCCAC CTTGGAGGCG 
CTGGGGGTGC ACCTGCAGAA CCCGAAGGAT GGCAAGTCGA TGCACCTGGA CGCGAGCAAG 
AAGATTGGGG ACTTCTTCAA CAAGGTGAAG AACAAGGCCG TCGACGTGGA GAAGATCCTG 
AAGGACAAGG ACAAGCACGG ACTCGTCCAG AAGTTCAAGG AGAAGACGGA GATCGTGGAG 
AAGAAGGTTC AGGCCAAGGT GGAGAGCTTG CAGAAGGCGG TGGAGTCGCA CGAGGAGCAG 
CAGGACCTGA AGAGCAAGAT CGCCTCCAAG ATGGAGGTCA CCCACGTGAT GGAGGTGGCC 
CAGCTGCTCC TCTCGCTGCT CCACTCGTGG GGCCTGGACC CGCACCTGGA CAAGATGTGT 
GAGACGCGGC TGGGCCTGCT CCGTCCCATC GTGCCCATCT CGTACGGGGT GCTCTCCAAG 
GCGGGCTACA TGTCCCTGTT GCTGCCCACT TGGCAGAACA ACTACGACAT TCCGCCGGGC 
CTCCAGCTGC CCTCCAGCTC GAAGAAGCGC CCGCTTCCGG AGGAGCTGCA GCGCCTGGAG 
CACCTGACCG CCATATTCAC TTCACGCCTC CACTGGGAGC TGAGCACCAC GCTGACGACT 
AACCACATCC TGGCGCTGGT AGCCATGTCG AACACGCTGC TGTCGATGAG CGCCGCCTCT 
TTCCTGCCGG ACAGCGAGAA GCACAAGAAG CTGCAGCGCC TGGCCCAGCG GACCGACTCC 
ACGCTGAGCA ACGAGGAGGA GCGCGAGGAG ATCCTCGCCC ACCACATTTC ACAGATCAAG 
CATGCCTGGA GCCTCCTGGC CACCCACCAC TGCTTCCTGT TGCCCGACAA AATCGAGGCC 
CTCGAGCCGA AGAAGTTCAA GCGACCCCAG GTAGAGATGA TGGTGAAGCG ATGGCAGCAC 
CACTGCATCG AAATCCGCGA GGCTGCCCAG CAGATCCTGC TCGGGGAGCT CACCCGCATG 
GGCAAGAAGG GGCGAAAGCA GCTAGTCGAA AGCTGGGCTC AGTACCTGCC CCTCTACACC 
CACACCGAGC CCATCGTGGG GGCCCAGCAG CAGCTGGTGC TCCTCAGCCA GAGCTCGAGC 
GGATCCCATG CCAGCGTCGG GGCTGGAGGC GGCGGCGGCA GCAACGGCAC CCACTCAATT 
GGTGGCTCAG CTGCGGGTGC CGGGGCGGGA GGAATTCCCG GCGCGGAACA GCACCATGAC 
GAGGACTACG AGGAGGAGGA GGAGGAGATC ATACGCAAGC CGTCTAGTCT GTCGGAGCTG 
AAACGCAAGC AAACCACGGC CGTCATCCTG CTGGGCGTGA TCGGGGCAGA GTTCGGGCAG 
GACATCTCAC AAGAGTCGCC CAACCACCGC GGGAGCATCA GCCTCGCGAC TGGGGCGACC 
GGACAGAGCG GAGAGCGCCG CATATCGTCC GTGGTGGAGG GCTTCGGCAT AGCCAATAAC 
CTGGCCAGGC TCACCTCGAT GGCCCTGGCC CACCTGCTGT ATGCGCCGCC CTCGCCCAAG 
CTGCCCCAGT ACACGCCGCT GCGGCGAGCG GCCATCGACC TGCTGGGCAG GGGCTTCACG 
GTGTGGGAGC CCTACCTGGA CGTGTCCAAG GTGCTGCTCG GCCTGCTGGA GATCTCGTGC 
GAGGGCAAAG CGGTGCCCAA TCTGAACTAC AAGCTTCCGC TGACGCCACA GGCGGACGCC 
TGCCGCACGG CCCGGCATGC CCTGCGCCTG ATAGCCACCG CCCGACCGGC GGCCTTCATC 
ACCACCATGG CCCGCGAGGT GGCCAGGTAC AACACGATGC AACAGAACGC GCAGTCCATC 
AACGCGCCCC TGACGCAGTC AGTCCTGTAC AAGGCCAAGG GCGAGATCCT GCAGTGCGTG 
GAGATGCTGA TCGACAAGAT GCAGTCGGAG ATCGCCAGCC TGCTGGTGGA GGTCATGGAC 
ATCGCGCTGC ACTGCGTGGA CAGCAACGAG CTGAAGGTCC GCGGCCTGGC CGAGCTCTGC 
CCGCCGATCT GCAAGTTCAA CCAGATCTCC CATTGCTCTC AGACCCGCCG CATCGCCGTC 
GGCGCCAACA GCGGCAACCT GGCCATCTAC GAGCTGCGTC AGAACAAGTG CCAGATGATC 
CCCGCTCACA CGCACCCCAT CACCTCCCTG GCCTTTTCGC CCGACGGCAA GTATCTCGTC 
TCGTACTCGT GCGCCGAGAA CCGGCTCTCC TTCTGGCAGA CGAGCACCGG CATGTTCGGG 
CTGGGACAGT CGCAGACGCG TTGCACCAAG GGCTACTCCA CGGCCCCCAT TCCGGACGTT 
TCGCGACTCA ACCCCATGCG GCTGGCCAAG CTTGTCTGGA TCAATAATCG TACGGTGACC 
CTCATGCTGG CTGACGGCTC CGAGACGCGG TTCAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354522.3
CDS	693..5294
Translation	MVSTNLVVPVVLWGPTAPTHCISSVFLSDDQCTLVTGCYDGQICLWQVEPSTLKMSPRCLLVGHSAPVLCLVRASLLPENNFLVSSSENGEMCTWDLIDGKCMEAVKLPQVHTQIQSYHTANSEDIRLFCIGYYAEIMVMDPFSLEVIYVLSSKVKPDWISAIHVLRPMRRKDDVVLAITTTGTVKVWTLTGNENKHAEPIYENESKEIRCLNAITMNCCAQNQRTVLLVCTKYWQIYDAGDFTVLCSVIAPARERWQGGDFITSDRVMLWTDEGKGYLYRLPANCIPDNKEFHSKSVVRDAPYLYYVLQHAGDKVLSCPPAMKLLLAGSNAIGNGNGNGSGGQHNLLRGDSEGYISVWNVPEVPLDNISILQAKQMPPRVLKPHVCTSLVEAWSIMDPPPVGILDQLSRITESPVKLTSSIYLPQQSRLVIGREDGSIVIVPATQTVMMQLLVGIKQNFSDWPSHQILYGHRGRVNCLLCPSMIHSRYEKSHLLSGGIDFAVCLWDLYSGSMLHRFCVHAGEITQLLVPPESCSPRILKCICSVASDHSVTLVSLQERKCVTLASRHLFPVVTIKWRPLDDFLIVGCSDGSVYVWQMETGHLDRVLHGMLAEEVLSACDEQAGEDGVGGGSSGVSGASTSEMGMANPAVHFFRGLKSRNMNAIRHATQRGITQLQQLQGHNQGNFDFLMKHRSNPLVIQGLRTNPKDAESHILFFDIEGLIFELHSEEYAQMTPATLEALGVHLQNPKDGKSMHLDASKKIGDFFNKVKNKAVDVEKILKDKDKHGLVQKFKEKTEIVEKKVQAKVESLQKAVESHEEQQDLKSKIASKMEVTHVMEVAQLLLSLLHSWGLDPHLDKMCETRLGLLRPIVPISYGVLSKAGYMSLLLPTWQNNYDIPPGLQLPSSSKKRPLPEELQRLEHLTAIFTSRLHWELSTTLTTNHILALVAMSNTLLSMSAASFLPDSEKHKKLQRLAQRTDSTLSNEEEREEILAHHISQIKHAWSLLATHHCFLLPDKIEALEPKKFKRPQVEMMVKRWQHHCIEIREAAQQILLGELTRMGKKGRKQLVESWAQYLPLYTHTEPIVGAQQQLVLLSQSSSGSHASVGAGGGGGSNGTHSIGGSAAGAGAGGIPGAEQHHDEDYEEEEEEIIRKPSSLSELKRKQTTAVILLGVIGAEFGQDISQESPNHRGSISLATGATGQSGERRISSVVEGFGIANNLARLTSMALAHLLYAPPSPKLPQYTPLRRAAIDLLGRGFTVWEPYLDVSKVLLGLLEISCEGKAVPNLNYKLPLTPQADACRTARHALRLIATARPAAFITTMAREVARYNTMQQNAQSINAPLTQSVLYKAKGEILQCVEMLIDKMQSEIASLLVEVMDIALHCVDSNELKVRGLAELCPPICKFNQISHCSQTRRIAVGANSGNLAIYELRQNKCQMIPAHTHPITSLAFSPDGKYLVSYSCAENRLSFWQTSTGMFGLGQSQTRCTKGYSTAPIPDVSRLNPMRLAKLVWINNRTVTLMLADGSETRFNV

Target ORF information:

RefSeq Version	XM_001354486.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA14656), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354486.3

ORF Insert Sequence:

ATGGTGAGTA CCAATCTGGT GGTGCCGGTG GTGCTGTGGG GACCCACGGC GCCCACGCAC 
TGCATCTCCA GTGTGTTTCT TTCGGACGAC CAGTGCACCC TGGTCACCGG CTGCTACGAC 
GGACAGATTT GTCTCTGGCA GGTGGAGCCC AGCACACTGA AGATGTCGCC CCGCTGCCTG 
CTGGTGGGCC ACTCGGCTCC GGTGCTCTGC CTGGTGCGGG CCTCGCTGCT GCCCGAAAAC 
AACTTCCTGG TGAGCTCCTC GGAGAACGGC GAGATGTGCA CCTGGGACCT CATCGACGGC 
AAGTGCATGG AGGCAGTCAA GCTGCCGCAG GTGCACACAC AGATCCAGAG CTATCACACG 
GCCAATAGCG AGGACATTCG TCTGTTCTGC ATCGGCTATT ACGCCGAGAT CATGGTGATG 
GACCCGTTCA GCCTGGAAGT AATCTACGTG CTCAGCTCCA AGGTGAAGCC AGACTGGATC 
TCGGCCATCC ACGTGCTCCG GCCCATGCGG CGCAAGGACG ACGTGGTCCT GGCCATCACC 
ACCACGGGCA CAGTGAAGGT GTGGACTCTG ACGGGGAACG AGAACAAGCA CGCGGAGCCC 
ATCTACGAGA ACGAGTCGAA GGAGATCCGC TGCCTCAACG CCATAACCAT GAACTGCTGT 
GCCCAGAACC AGCGCACTGT GCTCTTGGTG TGCACAAAGT ACTGGCAGAT CTACGACGCC 
GGGGACTTTA CCGTTCTCTG CTCCGTGATC GCGCCGGCCC GCGAGCGCTG GCAGGGCGGC 
GACTTCATCA CATCTGACCG CGTCATGCTC TGGACGGACG AGGGCAAGGG CTACCTGTAC 
AGGCTGCCCG CCAACTGCAT CCCCGACAAC AAGGAGTTCC ACTCGAAGAG CGTCGTCCGG 
GACGCCCCCT ACCTGTACTA CGTGCTCCAG CATGCCGGCG ACAAGGTACT CTCCTGTCCA 
CCAGCCATGA AACTGCTGCT GGCCGGAAGC AACGCAATAG GCAATGGAAA TGGCAACGGC 
AGTGGCGGAC AGCACAACCT GCTGCGCGGC GACTCTGAGG GGTACATCTC GGTGTGGAAT 
GTGCCGGAGG TGCCGCTGGA CAACATCAGC ATACTGCAGG CCAAGCAGAT GCCGCCGCGC 
GTCCTCAAGC CACACGTGTG CACTTCTCTG GTAGAGGCCT GGTCCATTAT GGATCCGCCG 
CCCGTGGGGA TACTGGATCA GCTGTCGAGG ATCACCGAGT CCCCGGTTAA ACTCACCTCG 
AGCATATACT TGCCGCAACA GAGTCGGCTG GTCATCGGGC GCGAGGATGG CAGCATTGTG 
ATCGTGCCCG CCACCCAGAC GGTGATGATG CAGTTGCTGG TTGGCATCAA GCAGAACTTC 
AGCGACTGGC CGTCGCACCA GATCTTGTAC GGGCACCGGG GCAGGGTGAA CTGCCTGCTC 
TGTCCCTCCA TGATCCACTC GCGGTACGAG AAGTCGCACC TGCTGTCCGG CGGCATCGAC 
TTTGCCGTCT GCCTCTGGGA CCTGTACAGC GGCAGCATGC TGCACCGCTT CTGCGTGCAC 
GCGGGGGAGA TCACCCAGCT GCTTGTGCCG CCGGAAAGCT GCAGCCCGCG GATCCTCAAG 
TGCATCTGCT CGGTCGCCTC GGACCACTCG GTGACTCTTG TCTCCCTGCA GGAGCGCAAG 
TGCGTTACCC TGGCCAGTCG CCACCTGTTC CCCGTGGTGA CCATCAAGTG GCGTCCACTG 
GACGACTTCC TCATCGTGGG CTGCTCGGAC GGCTCCGTCT ACGTGTGGCA GATGGAAACC 
GGCCACCTGG ACCGGGTGCT CCACGGAATG CTGGCCGAAG AGGTGCTCTC CGCCTGCGAC 
GAGCAGGCTG GCGAGGACGG GGTCGGTGGT GGCTCCTCGG GGGTGAGCGG CGCCTCGACC 
AGCGAAATGG GGATGGCCAA TCCGGCGGTT CACTTCTTCC GCGGCCTCAA GTCGAGGAAC 
ATGAACGCCA TCCGCCACGC CACCCAGCGC GGGATCACCC AGCTCCAGCA GCTGCAGGGC 
CACAACCAAG GGAACTTTGA CTTCCTCATG AAGCACCGCA GCAATCCACT GGTGATCCAG 
GGACTGCGCA CCAACCCCAA GGACGCCGAG AGCCACATCC TGTTCTTCGA CATCGAGGGA 
CTGATCTTCG AGCTGCACAG CGAGGAGTAC GCCCAGATGA CGCCGGCCAC CTTGGAGGCG 
CTGGGGGTGC ACCTGCAGAA CCCGAAGGAT GGCAAGTCGA TGCACCTGGA CGCGAGCAAG 
AAGATTGGGG ACTTCTTCAA CAAGGTGAAG AACAAGGCCG TCGACGTGGA GAAGATCCTG 
AAGGACAAGG ACAAGCACGG ACTCGTCCAG AAGTTCAAGG AGAAGACGGA GATCGTGGAG 
AAGAAGGTTC AGGCCAAGGT GGAGAGCTTG CAGAAGGCGG TGGAGTCGCA CGAGGAGCAG 
CAGGACCTGA AGAGCAAGAT CGCCTCCAAG ATGGAGGTCA CCCACGTGAT GGAGGTGGCC 
CAGCTGCTCC TCTCGCTGCT CCACTCGTGG GGCCTGGACC CGCACCTGGA CAAGATGTGT 
GAGACGCGGC TGGGCCTGCT CCGTCCCATC GTGCCCATCT CGTACGGGGT GCTCTCCAAG 
GCGGGCTACA TGTCCCTGTT GCTGCCCACT TGGCAGAACA ACTACGACAT TCCGCCGGGC 
CTCCAGCTGC CCTCCAGCTC GAAGAAGCGC CCGCTTCCGG AGGAGCTGCA GCGCCTGGAG 
CACCTGACCG CCATATTCAC TTCACGCCTC CACTGGGAGC TGAGCACCAC GCTGACGACT 
AACCACATCC TGGCGCTGGT AGCCATGTCG AACACGCTGC TGTCGATGAG CGCCGCCTCT 
TTCCTGCCGG ACAGCGAGAA GCACAAGAAG CTGCAGCGCC TGGCCCAGCG GACCGACTCC 
ACGCTGAGCA ACGAGGAGGA GCGCGAGGAG ATCCTCGCCC ACCACATTTC ACAGATCAAG 
CATGCCTGGA GCCTCCTGGC CACCCACCAC TGCTTCCTGT TGCCCGACAA AATCGAGGCC 
CTCGAGCCGA AGAAGTTCAA GCGACCCCAG GTAGAGATGA TGGTGAAGCG ATGGCAGCAC 
CACTGCATCG AAATCCGCGA GGCTGCCCAG CAGATCCTGC TCGGGGAGCT CACCCGCATG 
GGCAAGAAGG GGCGAAAGCA GCTAGTCGAA AGCTGGGCTC AGTACCTGCC CCTCTACACC 
CACACCGAGC CCATCGTGGG GGCCCAGCAG CAGCTGGTGC TCCTCAGCCA GAGCTCGAGC 
GGATCCCATG CCAGCGTCGG GGCTGGAGGC GGCGGCGGCA GCAACGGCAC CCACTCAATT 
GGTGGCTCAG CTGCGGGTGC CGGGGCGGGA GGAATTCCCG GCGCGGAACA GCACCATGAC 
GAGGACTACG AGGAGGAGGA GGAGGAGATC ATACGCAAGC CGTCTAGTCT GTCGGAGCTG 
AAACGCAAGC AAACCACGGC CGTCATCCTG CTGGGCGTGA TCGGGGCAGA GTTCGGGCAG 
GACATCTCAC AAGAGTCGCC CAACCACCGC GGGAGCATCA GCCTCGCGAC TGGGGCGACC 
GGACAGAGCG GAGAGCGCCG CATATCGTCC GTGGTGGAGG GCTTCGGCAT AGCCAATAAC 
CTGGCCAGGC TCACCTCGAT GGCCCTGGCC CACCTGCTGT ATGCGCCGCC CTCGCCCAAG 
CTGCCCCAGT ACACGCCGCT GCGGCGAGCG GCCATCGACC TGCTGGGCAG GGGCTTCACG 
GTGTGGGAGC CCTACCTGGA CGTGTCCAAG GTGCTGCTCG GCCTGCTGGA GATCTCGTGC 
GAGGGCAAAG CGGTGCCCAA TCTGAACTAC AAGCTTCCGC TGACGCCACA GGCGGACGCC 
TGCCGCACGG CCCGGCATGC CCTGCGCCTG ATAGCCACCG CCCGACCGGC GGCCTTCATC 
ACCACCATGG CCCGCGAGGT GGCCAGGTAC AACACGATGC AACAGAACGC GCAGTCCATC 
AACGCGCCCC TGACGCAGTC AGTCCTGTAC AAGGCCAAGG GCGAGATCCT GCAGTGCGTG 
GAGATGCTGA TCGACAAGAT GCAGTCGGAG ATCGCCAGCC TGCTGGTGGA GGTCATGGAC 
ATCGCGCTGC ACTGCGTGGA CAGCAACGAG CTGAAGGTCC GCGGCCTGGC CGAGCTCTGC 
CCGCCGATCT GCAAGTTCAA CCAGATCTCC CATTGCTCTC AGACCCGCCG CATCGCCGTC 
GGCGCCAACA GCGGCAACCT GGCCATCTAC GAGCTGCGTC AGAACAAGTG CCAGATGATC 
CCCGCTCACA CGCACCCCAT CACCTCCCTG GCCTTTTCGC CCGACGGCAA GTATCTCGTC 
TCGTACTCGT GCGCCGAGAA CCGGCTCTCC TTCTGGCAGA CGAGCACCGG CATGTTCGGG 
CTGGGACAGT CGCAGACGCG TTGCACCAAG GGCTACTCCA CGGCCCCCAT TCCGGACGTT 
TCGCGACTCA ACCCCATGCG GCTGGCCAAG CTTGTCTGGA TCAATAATCG TACGGTGACC 
CTCATGCTGG CTGACGGCTC CGAGACGCGG TTCAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA14656 ( XM_015185947.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015041433.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14656), mRNA.

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CloneID	ODe24288
Clone ID Related Accession (Same CDS sequence)	XM_001354486.3 , XM_015185947.1
Accession Version	XM_015185947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAGTA CCAATCTGGT GGTGCCGGTG GTGCTGTGGG GACCCACGGC GCCCACGCAC 
TGCATCTCCA GTGTGTTTCT TTCGGACGAC CAGTGCACCC TGGTCACCGG CTGCTACGAC 
GGACAGATTT GTCTCTGGCA GGTGGAGCCC AGCACACTGA AGATGTCGCC CCGCTGCCTG 
CTGGTGGGCC ACTCGGCTCC GGTGCTCTGC CTGGTGCGGG CCTCGCTGCT GCCCGAAAAC 
AACTTCCTGG TGAGCTCCTC GGAGAACGGC GAGATGTGCA CCTGGGACCT CATCGACGGC 
AAGTGCATGG AGGCAGTCAA GCTGCCGCAG GTGCACACAC AGATCCAGAG CTATCACACG 
GCCAATAGCG AGGACATTCG TCTGTTCTGC ATCGGCTATT ACGCCGAGAT CATGGTGATG 
GACCCGTTCA GCCTGGAAGT AATCTACGTG CTCAGCTCCA AGGTGAAGCC AGACTGGATC 
TCGGCCATCC ACGTGCTCCG GCCCATGCGG CGCAAGGACG ACGTGGTCCT GGCCATCACC 
ACCACGGGCA CAGTGAAGGT GTGGACTCTG ACGGGGAACG AGAACAAGCA CGCGGAGCCC 
ATCTACGAGA ACGAGTCGAA GGAGATCCGC TGCCTCAACG CCATAACCAT GAACTGCTGT 
GCCCAGAACC AGCGCACTGT GCTCTTGGTG TGCACAAAGT ACTGGCAGAT CTACGACGCC 
GGGGACTTTA CCGTTCTCTG CTCCGTGATC GCGCCGGCCC GCGAGCGCTG GCAGGGCGGC 
GACTTCATCA CATCTGACCG CGTCATGCTC TGGACGGACG AGGGCAAGGG CTACCTGTAC 
AGGCTGCCCG CCAACTGCAT CCCCGACAAC AAGGAGTTCC ACTCGAAGAG CGTCGTCCGG 
GACGCCCCCT ACCTGTACTA CGTGCTCCAG CATGCCGGCG ACAAGGTACT CTCCTGTCCA 
CCAGCCATGA AACTGCTGCT GGCCGGAAGC AACGCAATAG GCAATGGAAA TGGCAACGGC 
AGTGGCGGAC AGCACAACCT GCTGCGCGGC GACTCTGAGG GGTACATCTC GGTGTGGAAT 
GTGCCGGAGG TGCCGCTGGA CAACATCAGC ATACTGCAGG CCAAGCAGAT GCCGCCGCGC 
GTCCTCAAGC CACACGTGTG CACTTCTCTG GTAGAGGCCT GGTCCATTAT GGATCCGCCG 
CCCGTGGGGA TACTGGATCA GCTGTCGAGG ATCACCGAGT CCCCGGTTAA ACTCACCTCG 
AGCATATACT TGCCGCAACA GAGTCGGCTG GTCATCGGGC GCGAGGATGG CAGCATTGTG 
ATCGTGCCCG CCACCCAGAC GGTGATGATG CAGTTGCTGG TTGGCATCAA GCAGAACTTC 
AGCGACTGGC CGTCGCACCA GATCTTGTAC GGGCACCGGG GCAGGGTGAA CTGCCTGCTC 
TGTCCCTCCA TGATCCACTC GCGGTACGAG AAGTCGCACC TGCTGTCCGG CGGCATCGAC 
TTTGCCGTCT GCCTCTGGGA CCTGTACAGC GGCAGCATGC TGCACCGCTT CTGCGTGCAC 
GCGGGGGAGA TCACCCAGCT GCTTGTGCCG CCGGAAAGCT GCAGCCCGCG GATCCTCAAG 
TGCATCTGCT CGGTCGCCTC GGACCACTCG GTGACTCTTG TCTCCCTGCA GGAGCGCAAG 
TGCGTTACCC TGGCCAGTCG CCACCTGTTC CCCGTGGTGA CCATCAAGTG GCGTCCACTG 
GACGACTTCC TCATCGTGGG CTGCTCGGAC GGCTCCGTCT ACGTGTGGCA GATGGAAACC 
GGCCACCTGG ACCGGGTGCT CCACGGAATG CTGGCCGAAG AGGTGCTCTC CGCCTGCGAC 
GAGCAGGCTG GCGAGGACGG GGTCGGTGGT GGCTCCTCGG GGGTGAGCGG CGCCTCGACC 
AGCGAAATGG GGATGGCCAA TCCGGCGGTT CACTTCTTCC GCGGCCTCAA GTCGAGGAAC 
ATGAACGCCA TCCGCCACGC CACCCAGCGC GGGATCACCC AGCTCCAGCA GCTGCAGGGC 
CACAACCAAG GGAACTTTGA CTTCCTCATG AAGCACCGCA GCAATCCACT GGTGATCCAG 
GGACTGCGCA CCAACCCCAA GGACGCCGAG AGCCACATCC TGTTCTTCGA CATCGAGGGA 
CTGATCTTCG AGCTGCACAG CGAGGAGTAC GCCCAGATGA CGCCGGCCAC CTTGGAGGCG 
CTGGGGGTGC ACCTGCAGAA CCCGAAGGAT GGCAAGTCGA TGCACCTGGA CGCGAGCAAG 
AAGATTGGGG ACTTCTTCAA CAAGGTGAAG AACAAGGCCG TCGACGTGGA GAAGATCCTG 
AAGGACAAGG ACAAGCACGG ACTCGTCCAG AAGTTCAAGG AGAAGACGGA GATCGTGGAG 
AAGAAGGTTC AGGCCAAGGT GGAGAGCTTG CAGAAGGCGG TGGAGTCGCA CGAGGAGCAG 
CAGGACCTGA AGAGCAAGAT CGCCTCCAAG ATGGAGGTCA CCCACGTGAT GGAGGTGGCC 
CAGCTGCTCC TCTCGCTGCT CCACTCGTGG GGCCTGGACC CGCACCTGGA CAAGATGTGT 
GAGACGCGGC TGGGCCTGCT CCGTCCCATC GTGCCCATCT CGTACGGGGT GCTCTCCAAG 
GCGGGCTACA TGTCCCTGTT GCTGCCCACT TGGCAGAACA ACTACGACAT TCCGCCGGGC 
CTCCAGCTGC CCTCCAGCTC GAAGAAGCGC CCGCTTCCGG AGGAGCTGCA GCGCCTGGAG 
CACCTGACCG CCATATTCAC TTCACGCCTC CACTGGGAGC TGAGCACCAC GCTGACGACT 
AACCACATCC TGGCGCTGGT AGCCATGTCG AACACGCTGC TGTCGATGAG CGCCGCCTCT 
TTCCTGCCGG ACAGCGAGAA GCACAAGAAG CTGCAGCGCC TGGCCCAGCG GACCGACTCC 
ACGCTGAGCA ACGAGGAGGA GCGCGAGGAG ATCCTCGCCC ACCACATTTC ACAGATCAAG 
CATGCCTGGA GCCTCCTGGC CACCCACCAC TGCTTCCTGT TGCCCGACAA AATCGAGGCC 
CTCGAGCCGA AGAAGTTCAA GCGACCCCAG GTAGAGATGA TGGTGAAGCG ATGGCAGCAC 
CACTGCATCG AAATCCGCGA GGCTGCCCAG CAGATCCTGC TCGGGGAGCT CACCCGCATG 
GGCAAGAAGG GGCGAAAGCA GCTAGTCGAA AGCTGGGCTC AGTACCTGCC CCTCTACACC 
CACACCGAGC CCATCGTGGG GGCCCAGCAG CAGCTGGTGC TCCTCAGCCA GAGCTCGAGC 
GGATCCCATG CCAGCGTCGG GGCTGGAGGC GGCGGCGGCA GCAACGGCAC CCACTCAATT 
GGTGGCTCAG CTGCGGGTGC CGGGGCGGGA GGAATTCCCG GCGCGGAACA GCACCATGAC 
GAGGACTACG AGGAGGAGGA GGAGGAGATC ATACGCAAGC CGTCTAGTCT GTCGGAGCTG 
AAACGCAAGC AAACCACGGC CGTCATCCTG CTGGGCGTGA TCGGGGCAGA GTTCGGGCAG 
GACATCTCAC AAGAGTCGCC CAACCACCGC GGGAGCATCA GCCTCGCGAC TGGGGCGACC 
GGACAGAGCG GAGAGCGCCG CATATCGTCC GTGGTGGAGG GCTTCGGCAT AGCCAATAAC 
CTGGCCAGGC TCACCTCGAT GGCCCTGGCC CACCTGCTGT ATGCGCCGCC CTCGCCCAAG 
CTGCCCCAGT ACACGCCGCT GCGGCGAGCG GCCATCGACC TGCTGGGCAG GGGCTTCACG 
GTGTGGGAGC CCTACCTGGA CGTGTCCAAG GTGCTGCTCG GCCTGCTGGA GATCTCGTGC 
GAGGGCAAAG CGGTGCCCAA TCTGAACTAC AAGCTTCCGC TGACGCCACA GGCGGACGCC 
TGCCGCACGG CCCGGCATGC CCTGCGCCTG ATAGCCACCG CCCGACCGGC GGCCTTCATC 
ACCACCATGG CCCGCGAGGT GGCCAGGTAC AACACGATGC AACAGAACGC GCAGTCCATC 
AACGCGCCCC TGACGCAGTC AGTCCTGTAC AAGGCCAAGG GCGAGATCCT GCAGTGCGTG 
GAGATGCTGA TCGACAAGAT GCAGTCGGAG ATCGCCAGCC TGCTGGTGGA GGTCATGGAC 
ATCGCGCTGC ACTGCGTGGA CAGCAACGAG CTGAAGGTCC GCGGCCTGGC CGAGCTCTGC 
CCGCCGATCT GCAAGTTCAA CCAGATCTCC CATTGCTCTC AGACCCGCCG CATCGCCGTC 
GGCGCCAACA GCGGCAACCT GGCCATCTAC GAGCTGCGTC AGAACAAGTG CCAGATGATC 
CCCGCTCACA CGCACCCCAT CACCTCCCTG GCCTTTTCGC CCGACGGCAA GTATCTCGTC 
TCGTACTCGT GCGCCGAGAA CCGGCTCTCC TTCTGGCAGA CGAGCACCGG CATGTTCGGG 
CTGGGACAGT CGCAGACGCG TTGCACCAAG GGCTACTCCA CGGCCCCCAT TCCGGACGTT 
TCGCGACTCA ACCCCATGCG GCTGGCCAAG CTTGTCTGGA TCAATAATCG TACGGTGACC 
CTCATGCTGG CTGACGGCTC CGAGACGCGG TTCAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015041433.1
CDS	693..5294
Translation	MVSTNLVVPVVLWGPTAPTHCISSVFLSDDQCTLVTGCYDGQICLWQVEPSTLKMSPRCLLVGHSAPVLCLVRASLLPENNFLVSSSENGEMCTWDLIDGKCMEAVKLPQVHTQIQSYHTANSEDIRLFCIGYYAEIMVMDPFSLEVIYVLSSKVKPDWISAIHVLRPMRRKDDVVLAITTTGTVKVWTLTGNENKHAEPIYENESKEIRCLNAITMNCCAQNQRTVLLVCTKYWQIYDAGDFTVLCSVIAPARERWQGGDFITSDRVMLWTDEGKGYLYRLPANCIPDNKEFHSKSVVRDAPYLYYVLQHAGDKVLSCPPAMKLLLAGSNAIGNGNGNGSGGQHNLLRGDSEGYISVWNVPEVPLDNISILQAKQMPPRVLKPHVCTSLVEAWSIMDPPPVGILDQLSRITESPVKLTSSIYLPQQSRLVIGREDGSIVIVPATQTVMMQLLVGIKQNFSDWPSHQILYGHRGRVNCLLCPSMIHSRYEKSHLLSGGIDFAVCLWDLYSGSMLHRFCVHAGEITQLLVPPESCSPRILKCICSVASDHSVTLVSLQERKCVTLASRHLFPVVTIKWRPLDDFLIVGCSDGSVYVWQMETGHLDRVLHGMLAEEVLSACDEQAGEDGVGGGSSGVSGASTSEMGMANPAVHFFRGLKSRNMNAIRHATQRGITQLQQLQGHNQGNFDFLMKHRSNPLVIQGLRTNPKDAESHILFFDIEGLIFELHSEEYAQMTPATLEALGVHLQNPKDGKSMHLDASKKIGDFFNKVKNKAVDVEKILKDKDKHGLVQKFKEKTEIVEKKVQAKVESLQKAVESHEEQQDLKSKIASKMEVTHVMEVAQLLLSLLHSWGLDPHLDKMCETRLGLLRPIVPISYGVLSKAGYMSLLLPTWQNNYDIPPGLQLPSSSKKRPLPEELQRLEHLTAIFTSRLHWELSTTLTTNHILALVAMSNTLLSMSAASFLPDSEKHKKLQRLAQRTDSTLSNEEEREEILAHHISQIKHAWSLLATHHCFLLPDKIEALEPKKFKRPQVEMMVKRWQHHCIEIREAAQQILLGELTRMGKKGRKQLVESWAQYLPLYTHTEPIVGAQQQLVLLSQSSSGSHASVGAGGGGGSNGTHSIGGSAAGAGAGGIPGAEQHHDEDYEEEEEEIIRKPSSLSELKRKQTTAVILLGVIGAEFGQDISQESPNHRGSISLATGATGQSGERRISSVVEGFGIANNLARLTSMALAHLLYAPPSPKLPQYTPLRRAAIDLLGRGFTVWEPYLDVSKVLLGLLEISCEGKAVPNLNYKLPLTPQADACRTARHALRLIATARPAAFITTMAREVARYNTMQQNAQSINAPLTQSVLYKAKGEILQCVEMLIDKMQSEIASLLVEVMDIALHCVDSNELKVRGLAELCPPICKFNQISHCSQTRRIAVGANSGNLAIYELRQNKCQMIPAHTHPITSLAFSPDGKYLVSYSCAENRLSFWQTSTGMFGLGQSQTRCTKGYSTAPIPDVSRLNPMRLAKLVWINNRTVTLMLADGSETRFNV

Target ORF information:

RefSeq Version	XM_015185947.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14656), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015185947.1

ORF Insert Sequence:

ATGGTGAGTA CCAATCTGGT GGTGCCGGTG GTGCTGTGGG GACCCACGGC GCCCACGCAC 
TGCATCTCCA GTGTGTTTCT TTCGGACGAC CAGTGCACCC TGGTCACCGG CTGCTACGAC 
GGACAGATTT GTCTCTGGCA GGTGGAGCCC AGCACACTGA AGATGTCGCC CCGCTGCCTG 
CTGGTGGGCC ACTCGGCTCC GGTGCTCTGC CTGGTGCGGG CCTCGCTGCT GCCCGAAAAC 
AACTTCCTGG TGAGCTCCTC GGAGAACGGC GAGATGTGCA CCTGGGACCT CATCGACGGC 
AAGTGCATGG AGGCAGTCAA GCTGCCGCAG GTGCACACAC AGATCCAGAG CTATCACACG 
GCCAATAGCG AGGACATTCG TCTGTTCTGC ATCGGCTATT ACGCCGAGAT CATGGTGATG 
GACCCGTTCA GCCTGGAAGT AATCTACGTG CTCAGCTCCA AGGTGAAGCC AGACTGGATC 
TCGGCCATCC ACGTGCTCCG GCCCATGCGG CGCAAGGACG ACGTGGTCCT GGCCATCACC 
ACCACGGGCA CAGTGAAGGT GTGGACTCTG ACGGGGAACG AGAACAAGCA CGCGGAGCCC 
ATCTACGAGA ACGAGTCGAA GGAGATCCGC TGCCTCAACG CCATAACCAT GAACTGCTGT 
GCCCAGAACC AGCGCACTGT GCTCTTGGTG TGCACAAAGT ACTGGCAGAT CTACGACGCC 
GGGGACTTTA CCGTTCTCTG CTCCGTGATC GCGCCGGCCC GCGAGCGCTG GCAGGGCGGC 
GACTTCATCA CATCTGACCG CGTCATGCTC TGGACGGACG AGGGCAAGGG CTACCTGTAC 
AGGCTGCCCG CCAACTGCAT CCCCGACAAC AAGGAGTTCC ACTCGAAGAG CGTCGTCCGG 
GACGCCCCCT ACCTGTACTA CGTGCTCCAG CATGCCGGCG ACAAGGTACT CTCCTGTCCA 
CCAGCCATGA AACTGCTGCT GGCCGGAAGC AACGCAATAG GCAATGGAAA TGGCAACGGC 
AGTGGCGGAC AGCACAACCT GCTGCGCGGC GACTCTGAGG GGTACATCTC GGTGTGGAAT 
GTGCCGGAGG TGCCGCTGGA CAACATCAGC ATACTGCAGG CCAAGCAGAT GCCGCCGCGC 
GTCCTCAAGC CACACGTGTG CACTTCTCTG GTAGAGGCCT GGTCCATTAT GGATCCGCCG 
CCCGTGGGGA TACTGGATCA GCTGTCGAGG ATCACCGAGT CCCCGGTTAA ACTCACCTCG 
AGCATATACT TGCCGCAACA GAGTCGGCTG GTCATCGGGC GCGAGGATGG CAGCATTGTG 
ATCGTGCCCG CCACCCAGAC GGTGATGATG CAGTTGCTGG TTGGCATCAA GCAGAACTTC 
AGCGACTGGC CGTCGCACCA GATCTTGTAC GGGCACCGGG GCAGGGTGAA CTGCCTGCTC 
TGTCCCTCCA TGATCCACTC GCGGTACGAG AAGTCGCACC TGCTGTCCGG CGGCATCGAC 
TTTGCCGTCT GCCTCTGGGA CCTGTACAGC GGCAGCATGC TGCACCGCTT CTGCGTGCAC 
GCGGGGGAGA TCACCCAGCT GCTTGTGCCG CCGGAAAGCT GCAGCCCGCG GATCCTCAAG 
TGCATCTGCT CGGTCGCCTC GGACCACTCG GTGACTCTTG TCTCCCTGCA GGAGCGCAAG 
TGCGTTACCC TGGCCAGTCG CCACCTGTTC CCCGTGGTGA CCATCAAGTG GCGTCCACTG 
GACGACTTCC TCATCGTGGG CTGCTCGGAC GGCTCCGTCT ACGTGTGGCA GATGGAAACC 
GGCCACCTGG ACCGGGTGCT CCACGGAATG CTGGCCGAAG AGGTGCTCTC CGCCTGCGAC 
GAGCAGGCTG GCGAGGACGG GGTCGGTGGT GGCTCCTCGG GGGTGAGCGG CGCCTCGACC 
AGCGAAATGG GGATGGCCAA TCCGGCGGTT CACTTCTTCC GCGGCCTCAA GTCGAGGAAC 
ATGAACGCCA TCCGCCACGC CACCCAGCGC GGGATCACCC AGCTCCAGCA GCTGCAGGGC 
CACAACCAAG GGAACTTTGA CTTCCTCATG AAGCACCGCA GCAATCCACT GGTGATCCAG 
GGACTGCGCA CCAACCCCAA GGACGCCGAG AGCCACATCC TGTTCTTCGA CATCGAGGGA 
CTGATCTTCG AGCTGCACAG CGAGGAGTAC GCCCAGATGA CGCCGGCCAC CTTGGAGGCG 
CTGGGGGTGC ACCTGCAGAA CCCGAAGGAT GGCAAGTCGA TGCACCTGGA CGCGAGCAAG 
AAGATTGGGG ACTTCTTCAA CAAGGTGAAG AACAAGGCCG TCGACGTGGA GAAGATCCTG 
AAGGACAAGG ACAAGCACGG ACTCGTCCAG AAGTTCAAGG AGAAGACGGA GATCGTGGAG 
AAGAAGGTTC AGGCCAAGGT GGAGAGCTTG CAGAAGGCGG TGGAGTCGCA CGAGGAGCAG 
CAGGACCTGA AGAGCAAGAT CGCCTCCAAG ATGGAGGTCA CCCACGTGAT GGAGGTGGCC 
CAGCTGCTCC TCTCGCTGCT CCACTCGTGG GGCCTGGACC CGCACCTGGA CAAGATGTGT 
GAGACGCGGC TGGGCCTGCT CCGTCCCATC GTGCCCATCT CGTACGGGGT GCTCTCCAAG 
GCGGGCTACA TGTCCCTGTT GCTGCCCACT TGGCAGAACA ACTACGACAT TCCGCCGGGC 
CTCCAGCTGC CCTCCAGCTC GAAGAAGCGC CCGCTTCCGG AGGAGCTGCA GCGCCTGGAG 
CACCTGACCG CCATATTCAC TTCACGCCTC CACTGGGAGC TGAGCACCAC GCTGACGACT 
AACCACATCC TGGCGCTGGT AGCCATGTCG AACACGCTGC TGTCGATGAG CGCCGCCTCT 
TTCCTGCCGG ACAGCGAGAA GCACAAGAAG CTGCAGCGCC TGGCCCAGCG GACCGACTCC 
ACGCTGAGCA ACGAGGAGGA GCGCGAGGAG ATCCTCGCCC ACCACATTTC ACAGATCAAG 
CATGCCTGGA GCCTCCTGGC CACCCACCAC TGCTTCCTGT TGCCCGACAA AATCGAGGCC 
CTCGAGCCGA AGAAGTTCAA GCGACCCCAG GTAGAGATGA TGGTGAAGCG ATGGCAGCAC 
CACTGCATCG AAATCCGCGA GGCTGCCCAG CAGATCCTGC TCGGGGAGCT CACCCGCATG 
GGCAAGAAGG GGCGAAAGCA GCTAGTCGAA AGCTGGGCTC AGTACCTGCC CCTCTACACC 
CACACCGAGC CCATCGTGGG GGCCCAGCAG CAGCTGGTGC TCCTCAGCCA GAGCTCGAGC 
GGATCCCATG CCAGCGTCGG GGCTGGAGGC GGCGGCGGCA GCAACGGCAC CCACTCAATT 
GGTGGCTCAG CTGCGGGTGC CGGGGCGGGA GGAATTCCCG GCGCGGAACA GCACCATGAC 
GAGGACTACG AGGAGGAGGA GGAGGAGATC ATACGCAAGC CGTCTAGTCT GTCGGAGCTG 
AAACGCAAGC AAACCACGGC CGTCATCCTG CTGGGCGTGA TCGGGGCAGA GTTCGGGCAG 
GACATCTCAC AAGAGTCGCC CAACCACCGC GGGAGCATCA GCCTCGCGAC TGGGGCGACC 
GGACAGAGCG GAGAGCGCCG CATATCGTCC GTGGTGGAGG GCTTCGGCAT AGCCAATAAC 
CTGGCCAGGC TCACCTCGAT GGCCCTGGCC CACCTGCTGT ATGCGCCGCC CTCGCCCAAG 
CTGCCCCAGT ACACGCCGCT GCGGCGAGCG GCCATCGACC TGCTGGGCAG GGGCTTCACG 
GTGTGGGAGC CCTACCTGGA CGTGTCCAAG GTGCTGCTCG GCCTGCTGGA GATCTCGTGC 
GAGGGCAAAG CGGTGCCCAA TCTGAACTAC AAGCTTCCGC TGACGCCACA GGCGGACGCC 
TGCCGCACGG CCCGGCATGC CCTGCGCCTG ATAGCCACCG CCCGACCGGC GGCCTTCATC 
ACCACCATGG CCCGCGAGGT GGCCAGGTAC AACACGATGC AACAGAACGC GCAGTCCATC 
AACGCGCCCC TGACGCAGTC AGTCCTGTAC AAGGCCAAGG GCGAGATCCT GCAGTGCGTG 
GAGATGCTGA TCGACAAGAT GCAGTCGGAG ATCGCCAGCC TGCTGGTGGA GGTCATGGAC 
ATCGCGCTGC ACTGCGTGGA CAGCAACGAG CTGAAGGTCC GCGGCCTGGC CGAGCTCTGC 
CCGCCGATCT GCAAGTTCAA CCAGATCTCC CATTGCTCTC AGACCCGCCG CATCGCCGTC 
GGCGCCAACA GCGGCAACCT GGCCATCTAC GAGCTGCGTC AGAACAAGTG CCAGATGATC 
CCCGCTCACA CGCACCCCAT CACCTCCCTG GCCTTTTCGC CCGACGGCAA GTATCTCGTC 
TCGTACTCGT GCGCCGAGAA CCGGCTCTCC TTCTGGCAGA CGAGCACCGG CATGTTCGGG 
CTGGGACAGT CGCAGACGCG TTGCACCAAG GGCTACTCCA CGGCCCCCAT TCCGGACGTT 
TCGCGACTCA ACCCCATGCG GCTGGCCAAG CTTGTCTGGA TCAATAATCG TACGGTGACC 
CTCATGCTGG CTGACGGCTC CGAGACGCGG TTCAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA14656 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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