Dpse\GA10832 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10832
Entrez Gene ID	4804025
Full Name	GA10832 gene product from transcript GA10832-RD
Synonyms	GA10832,dpse_GLEANR_14584
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001360618.3	XP_001360655.2	uncharacterized protein, isoform A
XM_015184050.1	XP_015039536.1	uncharacterized protein, isoform B
XM_015184051.1	XP_015039537.1	uncharacterized protein, isoform C
XM_015184052.1	XP_015039538.1	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10832 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10832 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe03277	XM_001360618.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10832), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODe22671	XM_015184050.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10832), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODe22672	XM_015184051.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10832), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODe22673	XM_015184052.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA10832), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10832 ( XM_001360618.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001360655.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10832), mRNA.

Price & Availability↑

CloneID	ODe03277
Clone ID Related Accession (Same CDS sequence)	XM_001360618.3
Accession Version	XM_001360618.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001360618.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGATCA CACTATTAGT CACTCTGGCA GCATATATCG CGTTCCTCTT CACCCTGATC 
TTTGGCCGGG GCCAGAAAAG ACCAGTCCAC GCGCCATCCA CCAGAGCAGC CAGTGAAACA 
GAAACTACAA CATCGATTAA TCACAACTCC AGCGTGATTG CAGCTACAGC ACCACCAGTC 
ACAGCCACAA GCAGCTTGTC GTCGACCGCA ACGGGAGGAG CCACAACAAA GCTGAACGAG 
AAGCGATTCC CCAAAGGAGC CAGCATCAAG AAGGTTCAGT TCTCCAGCGA AAGCCTTAAG 
GAGGTGGATA TTGCCTTGTC CGATCTGAGC TATCACCAGA ACCACAACAA CAACACAACT 
GTCATCAAGA ACAAGAACAA CAATAATAGC ATTGTCGAAA TCAAAAAGAA ATCATCAGTC 
GAAATGAGCG AGGAAACAAA TGCAACGCCA ACAACGGATG CGGAGCGTCC AAGTTTCTTG 
GTGGGCGATG AGATTGACGA AAAGGCAGCC GAAGCCGGCG AGGCTGTAGA CGAGTTCCCC 
CAGAAGATGC AGAGCGCGTC CCGGCAGAAT GGCGATATAT CGGAGAAACG CAAGCGATTA 
AGCATGTTGA AGCGTCGCAC CAGTAAGCGT TTTGTACTTG TTGAGTCTGG CGAGGAGCCG 
CTGGTGCAGG CAGGAGCATC CGGATCGGGC CCTGTCATTC CACACTTTGT GGCCGTGGAC 
TGTGGACAGA GCGACAGCAA CCACAATGAG ATGAGTCCCA GCGCCAACAC CAGCCCCAGT 
AGCATCTCCA TCAACGGCGG TGACAGCAGC AACAACAACA ACCTCAGCAA CCCATCGCCA 
GGACTCCTTA GTGTGCCCAC CGTGGGAACC CTAAAACCGA GCATGTCGCG TGGCACTGGC 
CTGGGCCAGG ACCGCCATCA GGATCGTGAC TTTCATATAG CCACCACCGA GGAGTTTGTG 
AAGCGTTTCG GCGGTACACG TGTCATTAAC CGCGTCCTCA TTGCCAACAA TGGCATTGCG 
GCTGTCAAGT GCATGCGTTC CATTCGCCGA TGGGCCTACG AGATGTTCAA GAACGAGCGG 
GCCATCCGAT TTGTGGTGAT GGTCACCCCG GAGGACCTGA AGGCGAATGC CGAGTACATC 
AAGATGGCCG ATCACTATGT CCCCGTGCCC GGCGGTTCCA ACAACAACAA CTATGCGAAT 
GTTGAGCTCA TTGTGGACAT TGCACTTCGC ACACAAGTGC AGGCTGTCTG GGCTGGATGG 
GGTCATGCCT CGGAGAACCC CAAGTTGCCG GAGCTGCTGC ACAAGGAGGG TTTGGTGTTC 
CTCGGGCCAC CGGAGCGCGC CATGTGGGCG CTGGGCGACA AGGTGGCCTC CTCGATTGTG 
GCCCAAACGG CCGAAATACC CACACTACCA TGGTCCGGCT CCGACCTGAA AGCCCAGTAC 
AGCGGCAAGA AGATCAAGAT CACCAGCGAC CTTTTTGCCC GCGGCTGTGT GACTAATGTG 
GAGCAGGGTC TGGCGGCTGT CGCCAAGATT GGCTTTCCCG TGATGATCAA GGCTTCGGAA 
GGCGGCGGCG GCAAGGGCAT TCGTCGCGTG GACACCACCG AAGAGTTCCC CGCCCTGTTC 
CGTCAGGTGC AGGCCGAGGT GCCCGGCTCC CCCATCTTTG TGATGAAGCT GGCTCGCGGC 
GCACGCCACT TGGAAGTGCA GCTGCTGGCG GATCAGTACG GAAATGCCAT CAGTCTCTTT 
GGCCGCGATT GCTCCATACA GCGTCGCCAT CAAAAGATCA TCGAGGAGGC CCCGGCCATT 
GTGGCCCAGC CGGAGGTGTT TGAGGACATG GAAAAGGCGG CCGTGCGTCT GGCCAAGATG 
GTGGGCTATG TGAGCGCCGG CACCGTGGAG TACCTATACG ATCCGGAGGG CAGGTACTTC 
TTCCTGGAGC TGAACCCCCG TCTGCAGGTG GAGCATCCCT GCACGGAGAT GGTGGCCGAT 
GTCAATCTAC CGGCCTGCCA GCTGCAGATC GGCATGGGCA TCCCCCTATA CCGCCTCAAG 
GACATTCGCC TGTTGTACGG TGAGTCGCCG TGGGGCTCCT CCATCATTGA CTTTGAGAAT 
CCGCCGAACA AGCCCATGCC CTCGGGCCAT GTGATCGCTG CGCGCATCAC CTCCGAGAAT 
CCCGACGAGG GCTTCAAGCC CAGCTCGGGC ACAGTTCAGG AGCTCAACTT CCGCTCCAGC 
AAGAACGTCT GGGGCTACTT CAGTGTGGCC GCCTCCGGGG GATTGCACGA GTTTGCCGAC 
TCGCAGTTCG GTCACTGCTT CTCCTGGGGC GAGAACCGGC AGCAGGCGCG CGAGAATCTC 
GTGATTGCCC TCAAAGAGCT GTCGATCCGT GGCGATTTCC GCACCACCGT CGAGTACCTG 
ATCACGCTGC TCGAGACGAA TCGCTTCCTG GAGAACACCA TCGACACAGC CTGGCTGGAT 
GCTCTGATTG CCGAGCGCGT GCAGTCCGAG AAGCCGGACA TACTGCTGGG CGTGATGTGC 
GGGGCCCTGC ACATTGCCGA TCGCCAGATC ACCGAATCCT TCTCCAGCTT CCAAACGTCG 
CTGGAGAGGG GCCAGATCCA GGCGGCCAAC ACCCTGACGA ATGTGATAGA CGTGGAGCTG 
ATCAACGGAG GGATCCGGTA CAAGGTTCAA GCGGCCAAGA GCGGCGCCAA CTCGTACTTC 
CTGCTGATGA ACAGCTCCTT CAAGGAGATC GAGGTGCACC GCCTGTCCGA CGGCGGCCTG 
CTGATCTCCC TCGAGGGCGC CTCGTACACC ACCTACATGA AGGAGGAGGT GGACCGCTAC 
CGCATCGTTA TTGGCAACCA GACGTGCGTT TTCGAGAAGG AGAACGATCC GTCGCTGCTG 
CGCAGCCCAT CGGCGGGAAA GCTCATCAAT CTGATTGTGG AGGATGGGGC ACATGTGGCC 
AAGGGCCAGC CCTTTGCCGA GATCGAGGTG ATGAAGATGG TGATGACCCT CACCTCGCAG 
GAGGCAGGCA CGGTGACCTT TGTGCGGCGT CCAGGCGCCG TCCTCGACCC GGGCTCCCTG 
CTGGGCCACC TGGAGCTCGA TGATCCCTCG CTGGTGACCA AGGCGCAGCC CTGCAAGAGC 
CAGTTCCACC AGGCCGAGAA CGCGCCCATT CCCGAGAAGC TGAACCGCGT GCACAACACG 
TACAAGAGCA TTTTGGAGAA CACCTTGGCC GGCTACTGCC TGCCGGAGCC CTTCAATGCC 
CAGCGGCTGC GCGACATCAT CGAGAAGTTC ATGCAGAGCC TGAGGGATCC ATCGCTGCCG 
CTGCTGGAAC TCCAGGAGGT GATTGCCTCC ATCTCGGGCC GCATCCCCAT CTCCGTAGAG 
AAGAAGATTC GCAAGCTGAT GACCCTCTAC GAGCGGAACA TCACCAGTGT GTTGGCCCAG 
TTTCCCTCCC AGCAGATCGC CAGCGTCATC GACAGCCACG CGGCCACCTT GCAGAAGCGC 
GCCGATCGCG ACGTCTTCTT CCTGACCACC CAGAGCATTG TGCAGCTGGT GCAGCGCTAC 
CGGAATGGCA TTCGCGGCCG CATGAAGGCC GCCGTTCACG AACTGCTGCG CCAGTACTAC 
GACGTGGAGT CGCAGTTCCA GCACGGCCAC TACGACAAGT GCGTGGGCCT GGTGCGGGAG 
CACAACAAGG ATGACATGCT GACGGTGGTG AACACGATAT TCTCGCACTC GCAAGTGGCC 
AAGAAGAACT TGCTCGTTAC GCTGCTAATC GACCATCTGT GGGCCAACGA GCCGGGACTC 
ACCGACGAGC TGGCCAACAC CCTCAGCGAG CTAACGTCCC TCAACAGGGC CGAGCACTCG 
CGGGTGGCTC TGCGCTCTCG CCAGGTACTG ATTGCCGCCC ACCAGCCGGC ATACGAGCTG 
CGCCACAACC AAATGGAGTC CATATTCCTG TCGGCCGTAG ACATGTACGG CCACGACTTC 
CATCCGGAGA ATCTGCAGCG CCTGATCCTC TCGGAGACCT CAATCTTTGA CATTCTCCAC 
GATTTCTTCT ACCACACGAA CCGAGCCGTG TGCAACGCCG CCCTCGAGGT GTACGTGAGG 
CGCGCCTACA CCTCCTATGA GCTGACTTGC CTGCAGCACT TGGAGCTGTC CGGCGGCCTG 
CCGCTGGTGC ATTTCCAGTT CCTGCTGCCC ACGGCCCATC CGAATCGTCT GTTCTCGCGC 
ATGTCCTCGC CGGAGGGACT AGACCAGGCA GCCGCCGAGT CGATGGGCAG CTCCTTTGTC 
CGCACCGGAG CGATTGCCGC CTTCGATTCG TTCGAGCACT TTGAGATGTA CTCGGACGAG 
ATACTGGACC TTCTCGAGGA CTTTGTCTCG CCCGCGATGG TCAATGCCAA GGTGCTGGAG 
GCTGTGGAGG CAGCCGACTC CATCTCGGAC GGCCGTCACA GCACCTCCAT CAACGTCTCG 
CTGTCGGATC CCGTCACCAG AGCCAATGCC GCCGAGGAGG CCAAGTCCAC GGAGCCCATT 
CACATTGTGA GTGTGGCTGT GCGGGAGACC GGAGATATGG ACGACTTGCA GATGGCCCAG 
ATTTTCGGGA ACTACTGCAA GGAGCACAAC GAGGAGCTCT TCCAGCGCCG CATTCGGAGG 
ATAACGTTTG CCGCTTTGAA GAAGCGCCAG TTCCCCAAGT TCTTTACATA CCGCGCACGC 
GACAAGTTCG AGGAGGATCG CATTTATCGG CATCTGGAGC CGGCCTCTGC CTTCCATTTG 
GAGCTAAACC GCATGAAGAC CTACAACCTG GAGGCCCTGC CGACGGCCAA CCAGAAGATG 
CATCTGTATT TGGGCAGGGC AAAGGTGTCC AAGGGACAGG AGGTGACCGA CTATCGTTTC 
TTCATCCGCT CGATCATCCG CCACTCGGAC CTCATCACCA AGGAGGCCTC ATTCGAGTAC 
CTCCAGAACG AGGGCGAACG GGTGCTGCTG GAGGCCATGG ACGAGCTGGA GGTGGCCTTC 
TCCCACCCGC ATGCCAAGCG CACCGATTGC AATCACATCT TTCTCAATTT CGTGCCCACC 
GTTATTATGG ACCCGGCCAA GATCGAGGAG TCCGTCACCA AGATGATCAT GCGCTATGGC 
CCCCGCCTGT GGAAGCTGCG CGTCCTCCAG GCCGAGCTGA AGATGGTCAT CCGCCAGTCG 
CCGCAGTCGC CCACCCAAGC GGTGCGCCTC TGTATTGCCA ACGATTCTGG CTACTTCCTG 
GACATCTCCA TGTACACGGA GCAGACGGAG CCGGAGACGG GAATTATCAA GTTCAAGGCC 
TATGGAGAGA AGCAGGGTTC CCTTCACGGC CATCCCATTT CGACGCCCTA CATGACCAAG 
GACTTCCTGC AGCAGAAGCG CTTCCAGGCG CAGTCGAGCG GCACCACGTA CGTGTACGAT 
GTGCCTGACA TGTTCCGCCA GATGACCGAG CGCCACTGGA AGGAGTTCTC CAATGCCCGG 
CCCTCCGTGG ACATACGCAT ACCCGACAAG ATTCTGATTG AATGCAAGGA GCTGGTGCTG 
GAGGGCGACA GCCTCATTGA GATGCAGCGT CTGCCGGGCG AAAACAATTG CGGCATGGTG 
GCCTGGCGCA TTGTCCTGGC CACGCCGGAG TACCCGAATG GGCGCGAGAT CATTGTGATA 
GCTAATGATC TGACCTACCT GATTGGCTCC TTTGGCATCA AAGAGGATGT GCTGTTCGCC 
AAGGCTTCCC AGTTGGCGCG CGAACGGAAA GTGCCCAGGA TCTATATATC GGTGAACAGC 
GGTGCCCGCA TTGGACTGGC CGAGGAGGTG AAAGCAATGT TCAAGATAGC ATGGGAGGAT 
CCCGAGGAGC CGGACAAGGG CTTCAAGTAC CTCTATCTGA GCACCGAGGA CTATGCCCAG 
GTGGCCAATC TCAATTCGGT GCGTGCCATT CTCATCGAGG ATGAGGGCGA GCAGCGCTAC 
AAAATCACCG ACATCATCGG CAAGGACGAT GGCCTGGGCG TCGAAAATCT GCGCTATGCC 
GGCCTGATTG CCGGCGAAAC GTCGCAGGCG TACGAGGAGA TTGTCACCAT TGCGATGGTC 
ACCTGCCGCA CCATCGGCAT CGGCTCCTAT GTGGTGCGTC TGGGCCAGCG TGTCATCCAG 
ATCGACAACT CGCACATTAT ACTCACGGGG TATGCGGCCC TGAATAAGCT GCTCGGTCGC 
AAGGTATACG CCTCAAACAA CCAATTGGGC GGCACGCAGA TCATGTTCAA CAATGGCGTG 
ACCCACAAAA CTGAGGCCAT CGACTTGGAC GGCGTGTATA CCATCCTCGA CTGGCTCTCG 
TACATTCCCG CCCACATTGG CTGCGATCTG CCCATCGTTT CGCCCAGCGA TCGTATCGAT 
CGGCCTGTCG ATTTTATGCC CACCAAGTCG CCGTACGATC CGCGCTGCAT GCTGGCCGGT 
CGTGTGAATC CCACCAATGC AAATGAGTGG GAGAATGGGT TCTTTGATCG TGATTCCTGG 
AGCGAGATTA TGGCCCCGTG GGCCAAGACC GTGGTCACGG GACGTGCTCG ACTGGGCGGT 
GTGCCTGTGG GTGTTATTGC CGTGGAGACG CGAACTGTGG AGGTGGAAAT GCCCGCGGAT 
CCGGCCAACC TGGATTCGGA AGCCAAGACA CTGCAGCAGG CGGGTCAGGT GTGGTATCCC 
GACTCATCCT ACAAGACGGC GCAAGCCATT AAGGACTTTG GGCGCGAGGA GTTGCCTCTG 
ATTGTGTTCG CCAACTGGCG TGGCTTCTCT GGCGGCATGA AGGACATGTA CGAGCAGATC 
GTCAAGTTTG GGGCCTACAT CGTTGACGGA CTGCGGGAGT ACAAGAAGCC GGTGCTCATC 
TACCTGCCGC CCAATGCCGA GCTGCGTGGC GGAGCCTGGG CCGTTCTGGA CTCGTTGATC 
AATCCGCGCT ACATGGAAAC CTATGCGGAT CCGGAGGCCC GAGGTGGTGT CCTCGAACCC 
GAGGGTATTG TGGAAATCAA ATACAAAGAG AAGGACCTCA TCAAGACGAT AAATCGTCTG 
GATGCGACCA CAATTGCCCT AAAGAAGGAA TTCGAAGAGG CAACTGCAGC TGGTGATAAC 
ACGAAGGCCG AGCTGGTGGA TGAGAAGATC AAGGCTCGTC TTGCGCAGCT AATGCATGTC 
TATCATACGG TGGCCGTGCA CTTTGCCGAC CTACACGACA CGCCCGAGCG CATGCTGGAG 
AAGGAGTGCA TCAGCGAGAT TGTGCCATGG CGCGGCTCGC GACGTTGGCT CTACTGGCGC 
CTGCGCCGCC TCCTGCTGGA GGATGCATAC ATCAAGAAGA TCTTGCGGGC ACAGGACAAT 
CTCTCTGTCG GACAGGCCAA GCAGATGCTC AGACGCTGGC TAGTCGAAGA AAAGGGTGCC 
ACAGAGGCAT ATCTGTGGGA CAAGAATGAG GAAATGGTGG CCTGGTATGA AGAGCAAACG 
AATACAGAAT CTATTGTCTC GCGCAATGTG AGTTCCGTGC GAAGGGATGC CATCATCTCA 
ACCATTTCGA AAATGCTCGA GGACTGTCCC GATGTAGCGC TGGACGCTGT GGTTGGCCTC 
TGTCAAGGCC TGACTCCCGT CAATCGTGGA GTCGTCGTAC GCACACTGGC GCAAATGCAG 
CTGAACGAAG AGTCCTCTAG CAATACCCAG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001360655.2
CDS	18..7673
Translation	MLITLLVTLAAYIAFLFTLIFGRGQKRPVHAPSTRAASETETTTSINHNSSVIAATAPPVTATSSLSSTATGGATTKLNEKRFPKGASIKKVQFSSESLKEVDIALSDLSYHQNHNNNTTVIKNKNNNNSIVEIKKKSSVEMSEETNATPTTDAERPSFLVGDEIDEKAAEAGEAVDEFPQKMQSASRQNGDISEKRKRLSMLKRRTSKRFVLVESGEEPLVQAGASGSGPVIPHFVAVDCGQSDSNHNEMSPSANTSPSSISINGGDSSNNNNLSNPSPGLLSVPTVGTLKPSMSRGTGLGQDRHQDRDFHIATTEEFVKRFGGTRVINRVLIANNGIAAVKCMRSIRRWAYEMFKNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGSNNNNYANVELIVDIALRTQVQAVWAGWGHASENPKLPELLHKEGLVFLGPPERAMWALGDKVASSIVAQTAEIPTLPWSGSDLKAQYSGKKIKITSDLFARGCVTNVEQGLAAVAKIGFPVMIKASEGGGGKGIRRVDTTEEFPALFRQVQAEVPGSPIFVMKLARGARHLEVQLLADQYGNAISLFGRDCSIQRRHQKIIEEAPAIVAQPEVFEDMEKAAVRLAKMVGYVSAGTVEYLYDPEGRYFFLELNPRLQVEHPCTEMVADVNLPACQLQIGMGIPLYRLKDIRLLYGESPWGSSIIDFENPPNKPMPSGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVAASGGLHEFADSQFGHCFSWGENRQQARENLVIALKELSIRGDFRTTVEYLITLLETNRFLENTIDTAWLDALIAERVQSEKPDILLGVMCGALHIADRQITESFSSFQTSLERGQIQAANTLTNVIDVELINGGIRYKVQAAKSGANSYFLLMNSSFKEIEVHRLSDGGLLISLEGASYTTYMKEEVDRYRIVIGNQTCVFEKENDPSLLRSPSAGKLINLIVEDGAHVAKGQPFAEIEVMKMVMTLTSQEAGTVTFVRRPGAVLDPGSLLGHLELDDPSLVTKAQPCKSQFHQAENAPIPEKLNRVHNTYKSILENTLAGYCLPEPFNAQRLRDIIEKFMQSLRDPSLPLLELQEVIASISGRIPISVEKKIRKLMTLYERNITSVLAQFPSQQIASVIDSHAATLQKRADRDVFFLTTQSIVQLVQRYRNGIRGRMKAAVHELLRQYYDVESQFQHGHYDKCVGLVREHNKDDMLTVVNTIFSHSQVAKKNLLVTLLIDHLWANEPGLTDELANTLSELTSLNRAEHSRVALRSRQVLIAAHQPAYELRHNQMESIFLSAVDMYGHDFHPENLQRLILSETSIFDILHDFFYHTNRAVCNAALEVYVRRAYTSYELTCLQHLELSGGLPLVHFQFLLPTAHPNRLFSRMSSPEGLDQAAAESMGSSFVRTGAIAAFDSFEHFEMYSDEILDLLEDFVSPAMVNAKVLEAVEAADSISDGRHSTSINVSLSDPVTRANAAEEAKSTEPIHIVSVAVRETGDMDDLQMAQIFGNYCKEHNEELFQRRIRRITFAALKKRQFPKFFTYRARDKFEEDRIYRHLEPASAFHLELNRMKTYNLEALPTANQKMHLYLGRAKVSKGQEVTDYRFFIRSIIRHSDLITKEASFEYLQNEGERVLLEAMDELEVAFSHPHAKRTDCNHIFLNFVPTVIMDPAKIEESVTKMIMRYGPRLWKLRVLQAELKMVIRQSPQSPTQAVRLCIANDSGYFLDISMYTEQTEPETGIIKFKAYGEKQGSLHGHPISTPYMTKDFLQQKRFQAQSSGTTYVYDVPDMFRQMTERHWKEFSNARPSVDIRIPDKILIECKELVLEGDSLIEMQRLPGENNCGMVAWRIVLATPEYPNGREIIVIANDLTYLIGSFGIKEDVLFAKASQLARERKVPRIYISVNSGARIGLAEEVKAMFKIAWEDPEEPDKGFKYLYLSTEDYAQVANLNSVRAILIEDEGEQRYKITDIIGKDDGLGVENLRYAGLIAGETSQAYEEIVTIAMVTCRTIGIGSYVVRLGQRVIQIDNSHIILTGYAALNKLLGRKVYASNNQLGGTQIMFNNGVTHKTEAIDLDGVYTILDWLSYIPAHIGCDLPIVSPSDRIDRPVDFMPTKSPYDPRCMLAGRVNPTNANEWENGFFDRDSWSEIMAPWAKTVVTGRARLGGVPVGVIAVETRTVEVEMPADPANLDSEAKTLQQAGQVWYPDSSYKTAQAIKDFGREELPLIVFANWRGFSGGMKDMYEQIVKFGAYIVDGLREYKKPVLIYLPPNAELRGGAWAVLDSLINPRYMETYADPEARGGVLEPEGIVEIKYKEKDLIKTINRLDATTIALKKEFEEATAAGDNTKAELVDEKIKARLAQLMHVYHTVAVHFADLHDTPERMLEKECISEIVPWRGSRRWLYWRLRRLLLEDAYIKKILRAQDNLSVGQAKQMLRRWLVEEKGATEAYLWDKNEEMVAWYEEQTNTESIVSRNVSSVRRDAIISTISKMLEDCPDVALDAVVGLCQGLTPVNRGVVVRTLAQMQLNEESSSNTQG

Target ORF information:

RefSeq Version	XM_001360618.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10832), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001360618.3

ORF Insert Sequence:

ATGTTGATCA CACTATTAGT CACTCTGGCA GCATATATCG CGTTCCTCTT CACCCTGATC 
TTTGGCCGGG GCCAGAAAAG ACCAGTCCAC GCGCCATCCA CCAGAGCAGC CAGTGAAACA 
GAAACTACAA CATCGATTAA TCACAACTCC AGCGTGATTG CAGCTACAGC ACCACCAGTC 
ACAGCCACAA GCAGCTTGTC GTCGACCGCA ACGGGAGGAG CCACAACAAA GCTGAACGAG 
AAGCGATTCC CCAAAGGAGC CAGCATCAAG AAGGTTCAGT TCTCCAGCGA AAGCCTTAAG 
GAGGTGGATA TTGCCTTGTC CGATCTGAGC TATCACCAGA ACCACAACAA CAACACAACT 
GTCATCAAGA ACAAGAACAA CAATAATAGC ATTGTCGAAA TCAAAAAGAA ATCATCAGTC 
GAAATGAGCG AGGAAACAAA TGCAACGCCA ACAACGGATG CGGAGCGTCC AAGTTTCTTG 
GTGGGCGATG AGATTGACGA AAAGGCAGCC GAAGCCGGCG AGGCTGTAGA CGAGTTCCCC 
CAGAAGATGC AGAGCGCGTC CCGGCAGAAT GGCGATATAT CGGAGAAACG CAAGCGATTA 
AGCATGTTGA AGCGTCGCAC CAGTAAGCGT TTTGTACTTG TTGAGTCTGG CGAGGAGCCG 
CTGGTGCAGG CAGGAGCATC CGGATCGGGC CCTGTCATTC CACACTTTGT GGCCGTGGAC 
TGTGGACAGA GCGACAGCAA CCACAATGAG ATGAGTCCCA GCGCCAACAC CAGCCCCAGT 
AGCATCTCCA TCAACGGCGG TGACAGCAGC AACAACAACA ACCTCAGCAA CCCATCGCCA 
GGACTCCTTA GTGTGCCCAC CGTGGGAACC CTAAAACCGA GCATGTCGCG TGGCACTGGC 
CTGGGCCAGG ACCGCCATCA GGATCGTGAC TTTCATATAG CCACCACCGA GGAGTTTGTG 
AAGCGTTTCG GCGGTACACG TGTCATTAAC CGCGTCCTCA TTGCCAACAA TGGCATTGCG 
GCTGTCAAGT GCATGCGTTC CATTCGCCGA TGGGCCTACG AGATGTTCAA GAACGAGCGG 
GCCATCCGAT TTGTGGTGAT GGTCACCCCG GAGGACCTGA AGGCGAATGC CGAGTACATC 
AAGATGGCCG ATCACTATGT CCCCGTGCCC GGCGGTTCCA ACAACAACAA CTATGCGAAT 
GTTGAGCTCA TTGTGGACAT TGCACTTCGC ACACAAGTGC AGGCTGTCTG GGCTGGATGG 
GGTCATGCCT CGGAGAACCC CAAGTTGCCG GAGCTGCTGC ACAAGGAGGG TTTGGTGTTC 
CTCGGGCCAC CGGAGCGCGC CATGTGGGCG CTGGGCGACA AGGTGGCCTC CTCGATTGTG 
GCCCAAACGG CCGAAATACC CACACTACCA TGGTCCGGCT CCGACCTGAA AGCCCAGTAC 
AGCGGCAAGA AGATCAAGAT CACCAGCGAC CTTTTTGCCC GCGGCTGTGT GACTAATGTG 
GAGCAGGGTC TGGCGGCTGT CGCCAAGATT GGCTTTCCCG TGATGATCAA GGCTTCGGAA 
GGCGGCGGCG GCAAGGGCAT TCGTCGCGTG GACACCACCG AAGAGTTCCC CGCCCTGTTC 
CGTCAGGTGC AGGCCGAGGT GCCCGGCTCC CCCATCTTTG TGATGAAGCT GGCTCGCGGC 
GCACGCCACT TGGAAGTGCA GCTGCTGGCG GATCAGTACG GAAATGCCAT CAGTCTCTTT 
GGCCGCGATT GCTCCATACA GCGTCGCCAT CAAAAGATCA TCGAGGAGGC CCCGGCCATT 
GTGGCCCAGC CGGAGGTGTT TGAGGACATG GAAAAGGCGG CCGTGCGTCT GGCCAAGATG 
GTGGGCTATG TGAGCGCCGG CACCGTGGAG TACCTATACG ATCCGGAGGG CAGGTACTTC 
TTCCTGGAGC TGAACCCCCG TCTGCAGGTG GAGCATCCCT GCACGGAGAT GGTGGCCGAT 
GTCAATCTAC CGGCCTGCCA GCTGCAGATC GGCATGGGCA TCCCCCTATA CCGCCTCAAG 
GACATTCGCC TGTTGTACGG TGAGTCGCCG TGGGGCTCCT CCATCATTGA CTTTGAGAAT 
CCGCCGAACA AGCCCATGCC CTCGGGCCAT GTGATCGCTG CGCGCATCAC CTCCGAGAAT 
CCCGACGAGG GCTTCAAGCC CAGCTCGGGC ACAGTTCAGG AGCTCAACTT CCGCTCCAGC 
AAGAACGTCT GGGGCTACTT CAGTGTGGCC GCCTCCGGGG GATTGCACGA GTTTGCCGAC 
TCGCAGTTCG GTCACTGCTT CTCCTGGGGC GAGAACCGGC AGCAGGCGCG CGAGAATCTC 
GTGATTGCCC TCAAAGAGCT GTCGATCCGT GGCGATTTCC GCACCACCGT CGAGTACCTG 
ATCACGCTGC TCGAGACGAA TCGCTTCCTG GAGAACACCA TCGACACAGC CTGGCTGGAT 
GCTCTGATTG CCGAGCGCGT GCAGTCCGAG AAGCCGGACA TACTGCTGGG CGTGATGTGC 
GGGGCCCTGC ACATTGCCGA TCGCCAGATC ACCGAATCCT TCTCCAGCTT CCAAACGTCG 
CTGGAGAGGG GCCAGATCCA GGCGGCCAAC ACCCTGACGA ATGTGATAGA CGTGGAGCTG 
ATCAACGGAG GGATCCGGTA CAAGGTTCAA GCGGCCAAGA GCGGCGCCAA CTCGTACTTC 
CTGCTGATGA ACAGCTCCTT CAAGGAGATC GAGGTGCACC GCCTGTCCGA CGGCGGCCTG 
CTGATCTCCC TCGAGGGCGC CTCGTACACC ACCTACATGA AGGAGGAGGT GGACCGCTAC 
CGCATCGTTA TTGGCAACCA GACGTGCGTT TTCGAGAAGG AGAACGATCC GTCGCTGCTG 
CGCAGCCCAT CGGCGGGAAA GCTCATCAAT CTGATTGTGG AGGATGGGGC ACATGTGGCC 
AAGGGCCAGC CCTTTGCCGA GATCGAGGTG ATGAAGATGG TGATGACCCT CACCTCGCAG 
GAGGCAGGCA CGGTGACCTT TGTGCGGCGT CCAGGCGCCG TCCTCGACCC GGGCTCCCTG 
CTGGGCCACC TGGAGCTCGA TGATCCCTCG CTGGTGACCA AGGCGCAGCC CTGCAAGAGC 
CAGTTCCACC AGGCCGAGAA CGCGCCCATT CCCGAGAAGC TGAACCGCGT GCACAACACG 
TACAAGAGCA TTTTGGAGAA CACCTTGGCC GGCTACTGCC TGCCGGAGCC CTTCAATGCC 
CAGCGGCTGC GCGACATCAT CGAGAAGTTC ATGCAGAGCC TGAGGGATCC ATCGCTGCCG 
CTGCTGGAAC TCCAGGAGGT GATTGCCTCC ATCTCGGGCC GCATCCCCAT CTCCGTAGAG 
AAGAAGATTC GCAAGCTGAT GACCCTCTAC GAGCGGAACA TCACCAGTGT GTTGGCCCAG 
TTTCCCTCCC AGCAGATCGC CAGCGTCATC GACAGCCACG CGGCCACCTT GCAGAAGCGC 
GCCGATCGCG ACGTCTTCTT CCTGACCACC CAGAGCATTG TGCAGCTGGT GCAGCGCTAC 
CGGAATGGCA TTCGCGGCCG CATGAAGGCC GCCGTTCACG AACTGCTGCG CCAGTACTAC 
GACGTGGAGT CGCAGTTCCA GCACGGCCAC TACGACAAGT GCGTGGGCCT GGTGCGGGAG 
CACAACAAGG ATGACATGCT GACGGTGGTG AACACGATAT TCTCGCACTC GCAAGTGGCC 
AAGAAGAACT TGCTCGTTAC GCTGCTAATC GACCATCTGT GGGCCAACGA GCCGGGACTC 
ACCGACGAGC TGGCCAACAC CCTCAGCGAG CTAACGTCCC TCAACAGGGC CGAGCACTCG 
CGGGTGGCTC TGCGCTCTCG CCAGGTACTG ATTGCCGCCC ACCAGCCGGC ATACGAGCTG 
CGCCACAACC AAATGGAGTC CATATTCCTG TCGGCCGTAG ACATGTACGG CCACGACTTC 
CATCCGGAGA ATCTGCAGCG CCTGATCCTC TCGGAGACCT CAATCTTTGA CATTCTCCAC 
GATTTCTTCT ACCACACGAA CCGAGCCGTG TGCAACGCCG CCCTCGAGGT GTACGTGAGG 
CGCGCCTACA CCTCCTATGA GCTGACTTGC CTGCAGCACT TGGAGCTGTC CGGCGGCCTG 
CCGCTGGTGC ATTTCCAGTT CCTGCTGCCC ACGGCCCATC CGAATCGTCT GTTCTCGCGC 
ATGTCCTCGC CGGAGGGACT AGACCAGGCA GCCGCCGAGT CGATGGGCAG CTCCTTTGTC 
CGCACCGGAG CGATTGCCGC CTTCGATTCG TTCGAGCACT TTGAGATGTA CTCGGACGAG 
ATACTGGACC TTCTCGAGGA CTTTGTCTCG CCCGCGATGG TCAATGCCAA GGTGCTGGAG 
GCTGTGGAGG CAGCCGACTC CATCTCGGAC GGCCGTCACA GCACCTCCAT CAACGTCTCG 
CTGTCGGATC CCGTCACCAG AGCCAATGCC GCCGAGGAGG CCAAGTCCAC GGAGCCCATT 
CACATTGTGA GTGTGGCTGT GCGGGAGACC GGAGATATGG ACGACTTGCA GATGGCCCAG 
ATTTTCGGGA ACTACTGCAA GGAGCACAAC GAGGAGCTCT TCCAGCGCCG CATTCGGAGG 
ATAACGTTTG CCGCTTTGAA GAAGCGCCAG TTCCCCAAGT TCTTTACATA CCGCGCACGC 
GACAAGTTCG AGGAGGATCG CATTTATCGG CATCTGGAGC CGGCCTCTGC CTTCCATTTG 
GAGCTAAACC GCATGAAGAC CTACAACCTG GAGGCCCTGC CGACGGCCAA CCAGAAGATG 
CATCTGTATT TGGGCAGGGC AAAGGTGTCC AAGGGACAGG AGGTGACCGA CTATCGTTTC 
TTCATCCGCT CGATCATCCG CCACTCGGAC CTCATCACCA AGGAGGCCTC ATTCGAGTAC 
CTCCAGAACG AGGGCGAACG GGTGCTGCTG GAGGCCATGG ACGAGCTGGA GGTGGCCTTC 
TCCCACCCGC ATGCCAAGCG CACCGATTGC AATCACATCT TTCTCAATTT CGTGCCCACC 
GTTATTATGG ACCCGGCCAA GATCGAGGAG TCCGTCACCA AGATGATCAT GCGCTATGGC 
CCCCGCCTGT GGAAGCTGCG CGTCCTCCAG GCCGAGCTGA AGATGGTCAT CCGCCAGTCG 
CCGCAGTCGC CCACCCAAGC GGTGCGCCTC TGTATTGCCA ACGATTCTGG CTACTTCCTG 
GACATCTCCA TGTACACGGA GCAGACGGAG CCGGAGACGG GAATTATCAA GTTCAAGGCC 
TATGGAGAGA AGCAGGGTTC CCTTCACGGC CATCCCATTT CGACGCCCTA CATGACCAAG 
GACTTCCTGC AGCAGAAGCG CTTCCAGGCG CAGTCGAGCG GCACCACGTA CGTGTACGAT 
GTGCCTGACA TGTTCCGCCA GATGACCGAG CGCCACTGGA AGGAGTTCTC CAATGCCCGG 
CCCTCCGTGG ACATACGCAT ACCCGACAAG ATTCTGATTG AATGCAAGGA GCTGGTGCTG 
GAGGGCGACA GCCTCATTGA GATGCAGCGT CTGCCGGGCG AAAACAATTG CGGCATGGTG 
GCCTGGCGCA TTGTCCTGGC CACGCCGGAG TACCCGAATG GGCGCGAGAT CATTGTGATA 
GCTAATGATC TGACCTACCT GATTGGCTCC TTTGGCATCA AAGAGGATGT GCTGTTCGCC 
AAGGCTTCCC AGTTGGCGCG CGAACGGAAA GTGCCCAGGA TCTATATATC GGTGAACAGC 
GGTGCCCGCA TTGGACTGGC CGAGGAGGTG AAAGCAATGT TCAAGATAGC ATGGGAGGAT 
CCCGAGGAGC CGGACAAGGG CTTCAAGTAC CTCTATCTGA GCACCGAGGA CTATGCCCAG 
GTGGCCAATC TCAATTCGGT GCGTGCCATT CTCATCGAGG ATGAGGGCGA GCAGCGCTAC 
AAAATCACCG ACATCATCGG CAAGGACGAT GGCCTGGGCG TCGAAAATCT GCGCTATGCC 
GGCCTGATTG CCGGCGAAAC GTCGCAGGCG TACGAGGAGA TTGTCACCAT TGCGATGGTC 
ACCTGCCGCA CCATCGGCAT CGGCTCCTAT GTGGTGCGTC TGGGCCAGCG TGTCATCCAG 
ATCGACAACT CGCACATTAT ACTCACGGGG TATGCGGCCC TGAATAAGCT GCTCGGTCGC 
AAGGTATACG CCTCAAACAA CCAATTGGGC GGCACGCAGA TCATGTTCAA CAATGGCGTG 
ACCCACAAAA CTGAGGCCAT CGACTTGGAC GGCGTGTATA CCATCCTCGA CTGGCTCTCG 
TACATTCCCG CCCACATTGG CTGCGATCTG CCCATCGTTT CGCCCAGCGA TCGTATCGAT 
CGGCCTGTCG ATTTTATGCC CACCAAGTCG CCGTACGATC CGCGCTGCAT GCTGGCCGGT 
CGTGTGAATC CCACCAATGC AAATGAGTGG GAGAATGGGT TCTTTGATCG TGATTCCTGG 
AGCGAGATTA TGGCCCCGTG GGCCAAGACC GTGGTCACGG GACGTGCTCG ACTGGGCGGT 
GTGCCTGTGG GTGTTATTGC CGTGGAGACG CGAACTGTGG AGGTGGAAAT GCCCGCGGAT 
CCGGCCAACC TGGATTCGGA AGCCAAGACA CTGCAGCAGG CGGGTCAGGT GTGGTATCCC 
GACTCATCCT ACAAGACGGC GCAAGCCATT AAGGACTTTG GGCGCGAGGA GTTGCCTCTG 
ATTGTGTTCG CCAACTGGCG TGGCTTCTCT GGCGGCATGA AGGACATGTA CGAGCAGATC 
GTCAAGTTTG GGGCCTACAT CGTTGACGGA CTGCGGGAGT ACAAGAAGCC GGTGCTCATC 
TACCTGCCGC CCAATGCCGA GCTGCGTGGC GGAGCCTGGG CCGTTCTGGA CTCGTTGATC 
AATCCGCGCT ACATGGAAAC CTATGCGGAT CCGGAGGCCC GAGGTGGTGT CCTCGAACCC 
GAGGGTATTG TGGAAATCAA ATACAAAGAG AAGGACCTCA TCAAGACGAT AAATCGTCTG 
GATGCGACCA CAATTGCCCT AAAGAAGGAA TTCGAAGAGG CAACTGCAGC TGGTGATAAC 
ACGAAGGCCG AGCTGGTGGA TGAGAAGATC AAGGCTCGTC TTGCGCAGCT AATGCATGTC 
TATCATACGG TGGCCGTGCA CTTTGCCGAC CTACACGACA CGCCCGAGCG CATGCTGGAG 
AAGGAGTGCA TCAGCGAGAT TGTGCCATGG CGCGGCTCGC GACGTTGGCT CTACTGGCGC 
CTGCGCCGCC TCCTGCTGGA GGATGCATAC ATCAAGAAGA TCTTGCGGGC ACAGGACAAT 
CTCTCTGTCG GACAGGCCAA GCAGATGCTC AGACGCTGGC TAGTCGAAGA AAAGGGTGCC 
ACAGAGGCAT ATCTGTGGGA CAAGAATGAG GAAATGGTGG CCTGGTATGA AGAGCAAACG 
AATACAGAAT CTATTGTCTC GCGCAATGTG AGTTCCGTGC GAAGGGATGC CATCATCTCA 
ACCATTTCGA AAATGCTCGA GGACTGTCCC GATGTAGCGC TGGACGCTGT GGTTGGCCTC 
TGTCAAGGCC TGACTCCCGT CAATCGTGGA GTCGTCGTAC GCACACTGGC GCAAATGCAG 
CTGAACGAAG AGTCCTCTAG CAATACCCAG GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10832 ( XM_015184050.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039536.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10832), mRNA.

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CloneID	ODe22671
Clone ID Related Accession (Same CDS sequence)	XM_015184050.1
Accession Version	XM_015184050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGATCA CACTATTAGT CACTCTGGCA GCATATATCG CGTTCCTCTT CACCCTGATC 
TTTGGCCGGG GCCAGAAAAG ACCAGTCCAC GCGCCATCCA CCAGAGCAGC CAGTGAAACA 
GAAACTACAA CATCGATTAA TCACAACTCC AGCGTGATTG CAGCTACAGC ACCACCAGTC 
ACAGCCACAA GCAGCTTGTC GTCGACCGCA ACGGGAGGAG CCACAACAAA GCTGAACGAG 
AAGCGATTCC CCAAAGGAGC CAGCATCAAG AAGGTTCAGT TCTCCAGCGA AAGCCTTAAG 
GAGGTGGATA TTGCCTTGTC CGATCTGAGC TATCACCAGA ACCACAACAA CAACACAACT 
GTCATCAAGA ACAAGAACAA CAATAATAGC ATTGTCGAAA TCAAAAAGAA ATCATCAGTC 
GAAATGAGCG AGGAAACAAA TGCAACGCCA ACAACGGATG CGGAGCGTCC AAGTTTCTTG 
GTGGGCGATG AGATTGACGA AAAGGCAGCC GAAGCCGGCG AGGCTGTAGA CGAGTTCCCC 
CAGAAGATGC AGAGCGCGTC CCGGCAGAAT GGCGATATAT CGGAGAAACG CAAGCGATTA 
AGACCGAGCA TGTCGCGTGG CACTGGCCTG GGCCAGGACC GCCATCAGGA TCGTGACTTT 
CATATAGCCA CCACCGAGGA GTTTGTGAAG CGTTTCGGCG GTACACGTGT CATTAACCGC 
GTCCTCATTG CCAACAATGG CATTGCGGCT GTCAAGTGCA TGCGTTCCAT TCGCCGATGG 
GCCTACGAGA TGTTCAAGAA CGAGCGGGCC ATCCGATTTG TGGTGATGGT CACCCCGGAG 
GACCTGAAGG CGAATGCCGA GTACATCAAG ATGGCCGATC ACTATGTCCC CGTGCCCGGC 
GGTTCCAACA ACAACAACTA TGCGAATGTT GAGCTCATTG TGGACATTGC ACTTCGCACA 
CAAGTGCAGG CTGTCTGGGC TGGATGGGGT CATGCCTCGG AGAACCCCAA GTTGCCGGAG 
CTGCTGCACA AGGAGGGTTT GGTGTTCCTC GGGCCACCGG AGCGCGCCAT GTGGGCGCTG 
GGCGACAAGG TGGCCTCCTC GATTGTGGCC CAAACGGCCG AAATACCCAC ACTACCATGG 
TCCGGCTCCG ACCTGAAAGC CCAGTACAGC GGCAAGAAGA TCAAGATCAC CAGCGACCTT 
TTTGCCCGCG GCTGTGTGAC TAATGTGGAG CAGGGTCTGG CGGCTGTCGC CAAGATTGGC 
TTTCCCGTGA TGATCAAGGC TTCGGAAGGC GGCGGCGGCA AGGGCATTCG TCGCGTGGAC 
ACCACCGAAG AGTTCCCCGC CCTGTTCCGT CAGGTGCAGG CCGAGGTGCC CGGCTCCCCC 
ATCTTTGTGA TGAAGCTGGC TCGCGGCGCA CGCCACTTGG AAGTGCAGCT GCTGGCGGAT 
CAGTACGGAA ATGCCATCAG TCTCTTTGGC CGCGATTGCT CCATACAGCG TCGCCATCAA 
AAGATCATCG AGGAGGCCCC GGCCATTGTG GCCCAGCCGG AGGTGTTTGA GGACATGGAA 
AAGGCGGCCG TGCGTCTGGC CAAGATGGTG GGCTATGTGA GCGCCGGCAC CGTGGAGTAC 
CTATACGATC CGGAGGGCAG GTACTTCTTC CTGGAGCTGA ACCCCCGTCT GCAGGTGGAG 
CATCCCTGCA CGGAGATGGT GGCCGATGTC AATCTACCGG CCTGCCAGCT GCAGATCGGC 
ATGGGCATCC CCCTATACCG CCTCAAGGAC ATTCGCCTGT TGTACGGTGA GTCGCCGTGG 
GGCTCCTCCA TCATTGACTT TGAGAATCCG CCGAACAAGC CCATGCCCTC GGGCCATGTG 
ATCGCTGCGC GCATCACCTC CGAGAATCCC GACGAGGGCT TCAAGCCCAG CTCGGGCACA 
GTTCAGGAGC TCAACTTCCG CTCCAGCAAG AACGTCTGGG GCTACTTCAG TGTGGCCGCC 
TCCGGGGGAT TGCACGAGTT TGCCGACTCG CAGTTCGGTC ACTGCTTCTC CTGGGGCGAG 
AACCGGCAGC AGGCGCGCGA GAATCTCGTG ATTGCCCTCA AAGAGCTGTC GATCCGTGGC 
GATTTCCGCA CCACCGTCGA GTACCTGATC ACGCTGCTCG AGACGAATCG CTTCCTGGAG 
AACACCATCG ACACAGCCTG GCTGGATGCT CTGATTGCCG AGCGCGTGCA GTCCGAGAAG 
CCGGACATAC TGCTGGGCGT GATGTGCGGG GCCCTGCACA TTGCCGATCG CCAGATCACC 
GAATCCTTCT CCAGCTTCCA AACGTCGCTG GAGAGGGGCC AGATCCAGGC GGCCAACACC 
CTGACGAATG TGATAGACGT GGAGCTGATC AACGGAGGGA TCCGGTACAA GGTTCAAGCG 
GCCAAGAGCG GCGCCAACTC GTACTTCCTG CTGATGAACA GCTCCTTCAA GGAGATCGAG 
GTGCACCGCC TGTCCGACGG CGGCCTGCTG ATCTCCCTCG AGGGCGCCTC GTACACCACC 
TACATGAAGG AGGAGGTGGA CCGCTACCGC ATCGTTATTG GCAACCAGAC GTGCGTTTTC 
GAGAAGGAGA ACGATCCGTC GCTGCTGCGC AGCCCATCGG CGGGAAAGCT CATCAATCTG 
ATTGTGGAGG ATGGGGCACA TGTGGCCAAG GGCCAGCCCT TTGCCGAGAT CGAGGTGATG 
AAGATGGTGA TGACCCTCAC CTCGCAGGAG GCAGGCACGG TGACCTTTGT GCGGCGTCCA 
GGCGCCGTCC TCGACCCGGG CTCCCTGCTG GGCCACCTGG AGCTCGATGA TCCCTCGCTG 
GTGACCAAGG CGCAGCCCTG CAAGAGCCAG TTCCACCAGG CCGAGAACGC GCCCATTCCC 
GAGAAGCTGA ACCGCGTGCA CAACACGTAC AAGAGCATTT TGGAGAACAC CTTGGCCGGC 
TACTGCCTGC CGGAGCCCTT CAATGCCCAG CGGCTGCGCG ACATCATCGA GAAGTTCATG 
CAGAGCCTGA GGGATCCATC GCTGCCGCTG CTGGAACTCC AGGAGGTGAT TGCCTCCATC 
TCGGGCCGCA TCCCCATCTC CGTAGAGAAG AAGATTCGCA AGCTGATGAC CCTCTACGAG 
CGGAACATCA CCAGTGTGTT GGCCCAGTTT CCCTCCCAGC AGATCGCCAG CGTCATCGAC 
AGCCACGCGG CCACCTTGCA GAAGCGCGCC GATCGCGACG TCTTCTTCCT GACCACCCAG 
AGCATTGTGC AGCTGGTGCA GCGCTACCGG AATGGCATTC GCGGCCGCAT GAAGGCCGCC 
GTTCACGAAC TGCTGCGCCA GTACTACGAC GTGGAGTCGC AGTTCCAGCA CGGCCACTAC 
GACAAGTGCG TGGGCCTGGT GCGGGAGCAC AACAAGGATG ACATGCTGAC GGTGGTGAAC 
ACGATATTCT CGCACTCGCA AGTGGCCAAG AAGAACTTGC TCGTTACGCT GCTAATCGAC 
CATCTGTGGG CCAACGAGCC GGGACTCACC GACGAGCTGG CCAACACCCT CAGCGAGCTA 
ACGTCCCTCA ACAGGGCCGA GCACTCGCGG GTGGCTCTGC GCTCTCGCCA GGTACTGATT 
GCCGCCCACC AGCCGGCATA CGAGCTGCGC CACAACCAAA TGGAGTCCAT ATTCCTGTCG 
GCCGTAGACA TGTACGGCCA CGACTTCCAT CCGGAGAATC TGCAGCGCCT GATCCTCTCG 
GAGACCTCAA TCTTTGACAT TCTCCACGAT TTCTTCTACC ACACGAACCG AGCCGTGTGC 
AACGCCGCCC TCGAGGTGTA CGTGAGGCGC GCCTACACCT CCTATGAGCT GACTTGCCTG 
CAGCACTTGG AGCTGTCCGG CGGCCTGCCG CTGGTGCATT TCCAGTTCCT GCTGCCCACG 
GCCCATCCGA ATCGTCTGTT CTCGCGCATG TCCTCGCCGG AGGGACTAGA CCAGGCAGCC 
GCCGAGTCGA TGGGCAGCTC CTTTGTCCGC ACCGGAGCGA TTGCCGCCTT CGATTCGTTC 
GAGCACTTTG AGATGTACTC GGACGAGATA CTGGACCTTC TCGAGGACTT TGTCTCGCCC 
GCGATGGTCA ATGCCAAGGT GCTGGAGGCT GTGGAGGCAG CCGACTCCAT CTCGGACGGC 
CGTCACAGCA CCTCCATCAA CGTCTCGCTG TCGGATCCCG TCACCAGAGC CAATGCCGCC 
GAGGAGGCCA AGTCCACGGA GCCCATTCAC ATTGTGAGTG TGGCTGTGCG GGAGACCGGA 
GATATGGACG ACTTGCAGAT GGCCCAGATT TTCGGGAACT ACTGCAAGGA GCACAACGAG 
GAGCTCTTCC AGCGCCGCAT TCGGAGGATA ACGTTTGCCG CTTTGAAGAA GCGCCAGTTC 
CCCAAGTTCT TTACATACCG CGCACGCGAC AAGTTCGAGG AGGATCGCAT TTATCGGCAT 
CTGGAGCCGG CCTCTGCCTT CCATTTGGAG CTAAACCGCA TGAAGACCTA CAACCTGGAG 
GCCCTGCCGA CGGCCAACCA GAAGATGCAT CTGTATTTGG GCAGGGCAAA GGTGTCCAAG 
GGACAGGAGG TGACCGACTA TCGTTTCTTC ATCCGCTCGA TCATCCGCCA CTCGGACCTC 
ATCACCAAGG AGGCCTCATT CGAGTACCTC CAGAACGAGG GCGAACGGGT GCTGCTGGAG 
GCCATGGACG AGCTGGAGGT GGCCTTCTCC CACCCGCATG CCAAGCGCAC CGATTGCAAT 
CACATCTTTC TCAATTTCGT GCCCACCGTT ATTATGGACC CGGCCAAGAT CGAGGAGTCC 
GTCACCAAGA TGATCATGCG CTATGGCCCC CGCCTGTGGA AGCTGCGCGT CCTCCAGGCC 
GAGCTGAAGA TGGTCATCCG CCAGTCGCCG CAGTCGCCCA CCCAAGCGGT GCGCCTCTGT 
ATTGCCAACG ATTCTGGCTA CTTCCTGGAC ATCTCCATGT ACACGGAGCA GACGGAGCCG 
GAGACGGGAA TTATCAAGTT CAAGGCCTAT GGAGAGAAGC AGGGTTCCCT TCACGGCCAT 
CCCATTTCGA CGCCCTACAT GACCAAGGAC TTCCTGCAGC AGAAGCGCTT CCAGGCGCAG 
TCGAGCGGCA CCACGTACGT GTACGATGTG CCTGACATGT TCCGCCAGAT GACCGAGCGC 
CACTGGAAGG AGTTCTCCAA TGCCCGGCCC TCCGTGGACA TACGCATACC CGACAAGATT 
CTGATTGAAT GCAAGGAGCT GGTGCTGGAG GGCGACAGCC TCATTGAGAT GCAGCGTCTG 
CCGGGCGAAA ACAATTGCGG CATGGTGGCC TGGCGCATTG TCCTGGCCAC GCCGGAGTAC 
CCGAATGGGC GCGAGATCAT TGTGATAGCT AATGATCTGA CCTACCTGAT TGGCTCCTTT 
GGCATCAAAG AGGATGTGCT GTTCGCCAAG GCTTCCCAGT TGGCGCGCGA ACGGAAAGTG 
CCCAGGATCT ATATATCGGT GAACAGCGGT GCCCGCATTG GACTGGCCGA GGAGGTGAAA 
GCAATGTTCA AGATAGCATG GGAGGATCCC GAGGAGCCGG ACAAGGGCTT CAAGTACCTC 
TATCTGAGCA CCGAGGACTA TGCCCAGGTG GCCAATCTCA ATTCGGTGCG TGCCATTCTC 
ATCGAGGATG AGGGCGAGCA GCGCTACAAA ATCACCGACA TCATCGGCAA GGACGATGGC 
CTGGGCGTCG AAAATCTGCG CTATGCCGGC CTGATTGCCG GCGAAACGTC GCAGGCGTAC 
GAGGAGATTG TCACCATTGC GATGGTCACC TGCCGCACCA TCGGCATCGG CTCCTATGTG 
GTGCGTCTGG GCCAGCGTGT CATCCAGATC GACAACTCGC ACATTATACT CACGGGGTAT 
GCGGCCCTGA ATAAGCTGCT CGGTCGCAAG GTATACGCCT CAAACAACCA ATTGGGCGGC 
ACGCAGATCA TGTTCAACAA TGGCGTGACC CACAAAACTG AGGCCATCGA CTTGGACGGC 
GTGTATACCA TCCTCGACTG GCTCTCGTAC ATTCCCGCCC ACATTGGCTG CGATCTGCCC 
ATCGTTTCGC CCAGCGATCG TATCGATCGG CCTGTCGATT TTATGCCCAC CAAGTCGCCG 
TACGATCCGC GCTGCATGCT GGCCGGTCGT GTGAATCCCA CCAATGCAAA TGAGTGGGAG 
AATGGGTTCT TTGATCGTGA TTCCTGGAGC GAGATTATGG CCCCGTGGGC CAAGACCGTG 
GTCACGGGAC GTGCTCGACT GGGCGGTGTG CCTGTGGGTG TTATTGCCGT GGAGACGCGA 
ACTGTGGAGG TGGAAATGCC CGCGGATCCG GCCAACCTGG ATTCGGAAGC CAAGACACTG 
CAGCAGGCGG GTCAGGTGTG GTATCCCGAC TCATCCTACA AGACGGCGCA AGCCATTAAG 
GACTTTGGGC GCGAGGAGTT GCCTCTGATT GTGTTCGCCA ACTGGCGTGG CTTCTCTGGC 
GGCATGAAGG ACATGTACGA GCAGATCGTC AAGTTTGGGG CCTACATCGT TGACGGACTG 
CGGGAGTACA AGAAGCCGGT GCTCATCTAC CTGCCGCCCA ATGCCGAGCT GCGTGGCGGA 
GCCTGGGCCG TTCTGGACTC GTTGATCAAT CCGCGCTACA TGGAAACCTA TGCGGATCCG 
GAGGCCCGAG GTGGTGTCCT CGAACCCGAG GGTATTGTGG AAATCAAATA CAAAGAGAAG 
GACCTCATCA AGACGATAAA TCGTCTGGAT GCGACCACAA TTGCCCTAAA GAAGGAATTC 
GAAGAGGCAA CTGCAGCTGG TGATAACACG AAGGCCGAGC TGGTGGATGA GAAGATCAAG 
GCTCGTCTTG CGCAGCTAAT GCATGTCTAT CATACGGTGG CCGTGCACTT TGCCGACCTA 
CACGACACGC CCGAGCGCAT GCTGGAGAAG GAGTGCATCA GCGAGATTGT GCCATGGCGC 
GGCTCGCGAC GTTGGCTCTA CTGGCGCCTG CGCCGCCTCC TGCTGGAGGA TGCATACATC 
AAGAAGATCT TGCGGGCACA GGACAATCTC TCTGTCGGAC AGGCCAAGCA GATGCTCAGA 
CGCTGGCTAG TCGAAGAAAA GGGTGCCACA GAGGCATATC TGTGGGACAA GAATGAGGAA 
ATGGTGGCCT GGTATGAAGA GCAAACGAAT ACAGAATCTA TTGTCTCGCG CAATGTGAGT 
TCCGTGCGAA GGGATGCCAT CATCTCAACC ATTTCGAAAA TGCTCGAGGA CTGTCCCGAT 
GTAGCGCTGG ACGCTGTGGT TGGCCTCTGT CAAGGCCTGA CTCCCGTCAA TCGTGGAGTC 
GTCGTACGCA CACTGGCGCA AATGCAGCTG AACGAAGAGT CCTCTAGCAA TACCCAGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039536.1
CDS	180..7562
Translation	MLITLLVTLAAYIAFLFTLIFGRGQKRPVHAPSTRAASETETTTSINHNSSVIAATAPPVTATSSLSSTATGGATTKLNEKRFPKGASIKKVQFSSESLKEVDIALSDLSYHQNHNNNTTVIKNKNNNNSIVEIKKKSSVEMSEETNATPTTDAERPSFLVGDEIDEKAAEAGEAVDEFPQKMQSASRQNGDISEKRKRLRPSMSRGTGLGQDRHQDRDFHIATTEEFVKRFGGTRVINRVLIANNGIAAVKCMRSIRRWAYEMFKNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGSNNNNYANVELIVDIALRTQVQAVWAGWGHASENPKLPELLHKEGLVFLGPPERAMWALGDKVASSIVAQTAEIPTLPWSGSDLKAQYSGKKIKITSDLFARGCVTNVEQGLAAVAKIGFPVMIKASEGGGGKGIRRVDTTEEFPALFRQVQAEVPGSPIFVMKLARGARHLEVQLLADQYGNAISLFGRDCSIQRRHQKIIEEAPAIVAQPEVFEDMEKAAVRLAKMVGYVSAGTVEYLYDPEGRYFFLELNPRLQVEHPCTEMVADVNLPACQLQIGMGIPLYRLKDIRLLYGESPWGSSIIDFENPPNKPMPSGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVAASGGLHEFADSQFGHCFSWGENRQQARENLVIALKELSIRGDFRTTVEYLITLLETNRFLENTIDTAWLDALIAERVQSEKPDILLGVMCGALHIADRQITESFSSFQTSLERGQIQAANTLTNVIDVELINGGIRYKVQAAKSGANSYFLLMNSSFKEIEVHRLSDGGLLISLEGASYTTYMKEEVDRYRIVIGNQTCVFEKENDPSLLRSPSAGKLINLIVEDGAHVAKGQPFAEIEVMKMVMTLTSQEAGTVTFVRRPGAVLDPGSLLGHLELDDPSLVTKAQPCKSQFHQAENAPIPEKLNRVHNTYKSILENTLAGYCLPEPFNAQRLRDIIEKFMQSLRDPSLPLLELQEVIASISGRIPISVEKKIRKLMTLYERNITSVLAQFPSQQIASVIDSHAATLQKRADRDVFFLTTQSIVQLVQRYRNGIRGRMKAAVHELLRQYYDVESQFQHGHYDKCVGLVREHNKDDMLTVVNTIFSHSQVAKKNLLVTLLIDHLWANEPGLTDELANTLSELTSLNRAEHSRVALRSRQVLIAAHQPAYELRHNQMESIFLSAVDMYGHDFHPENLQRLILSETSIFDILHDFFYHTNRAVCNAALEVYVRRAYTSYELTCLQHLELSGGLPLVHFQFLLPTAHPNRLFSRMSSPEGLDQAAAESMGSSFVRTGAIAAFDSFEHFEMYSDEILDLLEDFVSPAMVNAKVLEAVEAADSISDGRHSTSINVSLSDPVTRANAAEEAKSTEPIHIVSVAVRETGDMDDLQMAQIFGNYCKEHNEELFQRRIRRITFAALKKRQFPKFFTYRARDKFEEDRIYRHLEPASAFHLELNRMKTYNLEALPTANQKMHLYLGRAKVSKGQEVTDYRFFIRSIIRHSDLITKEASFEYLQNEGERVLLEAMDELEVAFSHPHAKRTDCNHIFLNFVPTVIMDPAKIEESVTKMIMRYGPRLWKLRVLQAELKMVIRQSPQSPTQAVRLCIANDSGYFLDISMYTEQTEPETGIIKFKAYGEKQGSLHGHPISTPYMTKDFLQQKRFQAQSSGTTYVYDVPDMFRQMTERHWKEFSNARPSVDIRIPDKILIECKELVLEGDSLIEMQRLPGENNCGMVAWRIVLATPEYPNGREIIVIANDLTYLIGSFGIKEDVLFAKASQLARERKVPRIYISVNSGARIGLAEEVKAMFKIAWEDPEEPDKGFKYLYLSTEDYAQVANLNSVRAILIEDEGEQRYKITDIIGKDDGLGVENLRYAGLIAGETSQAYEEIVTIAMVTCRTIGIGSYVVRLGQRVIQIDNSHIILTGYAALNKLLGRKVYASNNQLGGTQIMFNNGVTHKTEAIDLDGVYTILDWLSYIPAHIGCDLPIVSPSDRIDRPVDFMPTKSPYDPRCMLAGRVNPTNANEWENGFFDRDSWSEIMAPWAKTVVTGRARLGGVPVGVIAVETRTVEVEMPADPANLDSEAKTLQQAGQVWYPDSSYKTAQAIKDFGREELPLIVFANWRGFSGGMKDMYEQIVKFGAYIVDGLREYKKPVLIYLPPNAELRGGAWAVLDSLINPRYMETYADPEARGGVLEPEGIVEIKYKEKDLIKTINRLDATTIALKKEFEEATAAGDNTKAELVDEKIKARLAQLMHVYHTVAVHFADLHDTPERMLEKECISEIVPWRGSRRWLYWRLRRLLLEDAYIKKILRAQDNLSVGQAKQMLRRWLVEEKGATEAYLWDKNEEMVAWYEEQTNTESIVSRNVSSVRRDAIISTISKMLEDCPDVALDAVVGLCQGLTPVNRGVVVRTLAQMQLNEESSSNTQG

Target ORF information:

RefSeq Version	XM_015184050.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10832), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184050.1

ORF Insert Sequence:

ATGTTGATCA CACTATTAGT CACTCTGGCA GCATATATCG CGTTCCTCTT CACCCTGATC 
TTTGGCCGGG GCCAGAAAAG ACCAGTCCAC GCGCCATCCA CCAGAGCAGC CAGTGAAACA 
GAAACTACAA CATCGATTAA TCACAACTCC AGCGTGATTG CAGCTACAGC ACCACCAGTC 
ACAGCCACAA GCAGCTTGTC GTCGACCGCA ACGGGAGGAG CCACAACAAA GCTGAACGAG 
AAGCGATTCC CCAAAGGAGC CAGCATCAAG AAGGTTCAGT TCTCCAGCGA AAGCCTTAAG 
GAGGTGGATA TTGCCTTGTC CGATCTGAGC TATCACCAGA ACCACAACAA CAACACAACT 
GTCATCAAGA ACAAGAACAA CAATAATAGC ATTGTCGAAA TCAAAAAGAA ATCATCAGTC 
GAAATGAGCG AGGAAACAAA TGCAACGCCA ACAACGGATG CGGAGCGTCC AAGTTTCTTG 
GTGGGCGATG AGATTGACGA AAAGGCAGCC GAAGCCGGCG AGGCTGTAGA CGAGTTCCCC 
CAGAAGATGC AGAGCGCGTC CCGGCAGAAT GGCGATATAT CGGAGAAACG CAAGCGATTA 
AGACCGAGCA TGTCGCGTGG CACTGGCCTG GGCCAGGACC GCCATCAGGA TCGTGACTTT 
CATATAGCCA CCACCGAGGA GTTTGTGAAG CGTTTCGGCG GTACACGTGT CATTAACCGC 
GTCCTCATTG CCAACAATGG CATTGCGGCT GTCAAGTGCA TGCGTTCCAT TCGCCGATGG 
GCCTACGAGA TGTTCAAGAA CGAGCGGGCC ATCCGATTTG TGGTGATGGT CACCCCGGAG 
GACCTGAAGG CGAATGCCGA GTACATCAAG ATGGCCGATC ACTATGTCCC CGTGCCCGGC 
GGTTCCAACA ACAACAACTA TGCGAATGTT GAGCTCATTG TGGACATTGC ACTTCGCACA 
CAAGTGCAGG CTGTCTGGGC TGGATGGGGT CATGCCTCGG AGAACCCCAA GTTGCCGGAG 
CTGCTGCACA AGGAGGGTTT GGTGTTCCTC GGGCCACCGG AGCGCGCCAT GTGGGCGCTG 
GGCGACAAGG TGGCCTCCTC GATTGTGGCC CAAACGGCCG AAATACCCAC ACTACCATGG 
TCCGGCTCCG ACCTGAAAGC CCAGTACAGC GGCAAGAAGA TCAAGATCAC CAGCGACCTT 
TTTGCCCGCG GCTGTGTGAC TAATGTGGAG CAGGGTCTGG CGGCTGTCGC CAAGATTGGC 
TTTCCCGTGA TGATCAAGGC TTCGGAAGGC GGCGGCGGCA AGGGCATTCG TCGCGTGGAC 
ACCACCGAAG AGTTCCCCGC CCTGTTCCGT CAGGTGCAGG CCGAGGTGCC CGGCTCCCCC 
ATCTTTGTGA TGAAGCTGGC TCGCGGCGCA CGCCACTTGG AAGTGCAGCT GCTGGCGGAT 
CAGTACGGAA ATGCCATCAG TCTCTTTGGC CGCGATTGCT CCATACAGCG TCGCCATCAA 
AAGATCATCG AGGAGGCCCC GGCCATTGTG GCCCAGCCGG AGGTGTTTGA GGACATGGAA 
AAGGCGGCCG TGCGTCTGGC CAAGATGGTG GGCTATGTGA GCGCCGGCAC CGTGGAGTAC 
CTATACGATC CGGAGGGCAG GTACTTCTTC CTGGAGCTGA ACCCCCGTCT GCAGGTGGAG 
CATCCCTGCA CGGAGATGGT GGCCGATGTC AATCTACCGG CCTGCCAGCT GCAGATCGGC 
ATGGGCATCC CCCTATACCG CCTCAAGGAC ATTCGCCTGT TGTACGGTGA GTCGCCGTGG 
GGCTCCTCCA TCATTGACTT TGAGAATCCG CCGAACAAGC CCATGCCCTC GGGCCATGTG 
ATCGCTGCGC GCATCACCTC CGAGAATCCC GACGAGGGCT TCAAGCCCAG CTCGGGCACA 
GTTCAGGAGC TCAACTTCCG CTCCAGCAAG AACGTCTGGG GCTACTTCAG TGTGGCCGCC 
TCCGGGGGAT TGCACGAGTT TGCCGACTCG CAGTTCGGTC ACTGCTTCTC CTGGGGCGAG 
AACCGGCAGC AGGCGCGCGA GAATCTCGTG ATTGCCCTCA AAGAGCTGTC GATCCGTGGC 
GATTTCCGCA CCACCGTCGA GTACCTGATC ACGCTGCTCG AGACGAATCG CTTCCTGGAG 
AACACCATCG ACACAGCCTG GCTGGATGCT CTGATTGCCG AGCGCGTGCA GTCCGAGAAG 
CCGGACATAC TGCTGGGCGT GATGTGCGGG GCCCTGCACA TTGCCGATCG CCAGATCACC 
GAATCCTTCT CCAGCTTCCA AACGTCGCTG GAGAGGGGCC AGATCCAGGC GGCCAACACC 
CTGACGAATG TGATAGACGT GGAGCTGATC AACGGAGGGA TCCGGTACAA GGTTCAAGCG 
GCCAAGAGCG GCGCCAACTC GTACTTCCTG CTGATGAACA GCTCCTTCAA GGAGATCGAG 
GTGCACCGCC TGTCCGACGG CGGCCTGCTG ATCTCCCTCG AGGGCGCCTC GTACACCACC 
TACATGAAGG AGGAGGTGGA CCGCTACCGC ATCGTTATTG GCAACCAGAC GTGCGTTTTC 
GAGAAGGAGA ACGATCCGTC GCTGCTGCGC AGCCCATCGG CGGGAAAGCT CATCAATCTG 
ATTGTGGAGG ATGGGGCACA TGTGGCCAAG GGCCAGCCCT TTGCCGAGAT CGAGGTGATG 
AAGATGGTGA TGACCCTCAC CTCGCAGGAG GCAGGCACGG TGACCTTTGT GCGGCGTCCA 
GGCGCCGTCC TCGACCCGGG CTCCCTGCTG GGCCACCTGG AGCTCGATGA TCCCTCGCTG 
GTGACCAAGG CGCAGCCCTG CAAGAGCCAG TTCCACCAGG CCGAGAACGC GCCCATTCCC 
GAGAAGCTGA ACCGCGTGCA CAACACGTAC AAGAGCATTT TGGAGAACAC CTTGGCCGGC 
TACTGCCTGC CGGAGCCCTT CAATGCCCAG CGGCTGCGCG ACATCATCGA GAAGTTCATG 
CAGAGCCTGA GGGATCCATC GCTGCCGCTG CTGGAACTCC AGGAGGTGAT TGCCTCCATC 
TCGGGCCGCA TCCCCATCTC CGTAGAGAAG AAGATTCGCA AGCTGATGAC CCTCTACGAG 
CGGAACATCA CCAGTGTGTT GGCCCAGTTT CCCTCCCAGC AGATCGCCAG CGTCATCGAC 
AGCCACGCGG CCACCTTGCA GAAGCGCGCC GATCGCGACG TCTTCTTCCT GACCACCCAG 
AGCATTGTGC AGCTGGTGCA GCGCTACCGG AATGGCATTC GCGGCCGCAT GAAGGCCGCC 
GTTCACGAAC TGCTGCGCCA GTACTACGAC GTGGAGTCGC AGTTCCAGCA CGGCCACTAC 
GACAAGTGCG TGGGCCTGGT GCGGGAGCAC AACAAGGATG ACATGCTGAC GGTGGTGAAC 
ACGATATTCT CGCACTCGCA AGTGGCCAAG AAGAACTTGC TCGTTACGCT GCTAATCGAC 
CATCTGTGGG CCAACGAGCC GGGACTCACC GACGAGCTGG CCAACACCCT CAGCGAGCTA 
ACGTCCCTCA ACAGGGCCGA GCACTCGCGG GTGGCTCTGC GCTCTCGCCA GGTACTGATT 
GCCGCCCACC AGCCGGCATA CGAGCTGCGC CACAACCAAA TGGAGTCCAT ATTCCTGTCG 
GCCGTAGACA TGTACGGCCA CGACTTCCAT CCGGAGAATC TGCAGCGCCT GATCCTCTCG 
GAGACCTCAA TCTTTGACAT TCTCCACGAT TTCTTCTACC ACACGAACCG AGCCGTGTGC 
AACGCCGCCC TCGAGGTGTA CGTGAGGCGC GCCTACACCT CCTATGAGCT GACTTGCCTG 
CAGCACTTGG AGCTGTCCGG CGGCCTGCCG CTGGTGCATT TCCAGTTCCT GCTGCCCACG 
GCCCATCCGA ATCGTCTGTT CTCGCGCATG TCCTCGCCGG AGGGACTAGA CCAGGCAGCC 
GCCGAGTCGA TGGGCAGCTC CTTTGTCCGC ACCGGAGCGA TTGCCGCCTT CGATTCGTTC 
GAGCACTTTG AGATGTACTC GGACGAGATA CTGGACCTTC TCGAGGACTT TGTCTCGCCC 
GCGATGGTCA ATGCCAAGGT GCTGGAGGCT GTGGAGGCAG CCGACTCCAT CTCGGACGGC 
CGTCACAGCA CCTCCATCAA CGTCTCGCTG TCGGATCCCG TCACCAGAGC CAATGCCGCC 
GAGGAGGCCA AGTCCACGGA GCCCATTCAC ATTGTGAGTG TGGCTGTGCG GGAGACCGGA 
GATATGGACG ACTTGCAGAT GGCCCAGATT TTCGGGAACT ACTGCAAGGA GCACAACGAG 
GAGCTCTTCC AGCGCCGCAT TCGGAGGATA ACGTTTGCCG CTTTGAAGAA GCGCCAGTTC 
CCCAAGTTCT TTACATACCG CGCACGCGAC AAGTTCGAGG AGGATCGCAT TTATCGGCAT 
CTGGAGCCGG CCTCTGCCTT CCATTTGGAG CTAAACCGCA TGAAGACCTA CAACCTGGAG 
GCCCTGCCGA CGGCCAACCA GAAGATGCAT CTGTATTTGG GCAGGGCAAA GGTGTCCAAG 
GGACAGGAGG TGACCGACTA TCGTTTCTTC ATCCGCTCGA TCATCCGCCA CTCGGACCTC 
ATCACCAAGG AGGCCTCATT CGAGTACCTC CAGAACGAGG GCGAACGGGT GCTGCTGGAG 
GCCATGGACG AGCTGGAGGT GGCCTTCTCC CACCCGCATG CCAAGCGCAC CGATTGCAAT 
CACATCTTTC TCAATTTCGT GCCCACCGTT ATTATGGACC CGGCCAAGAT CGAGGAGTCC 
GTCACCAAGA TGATCATGCG CTATGGCCCC CGCCTGTGGA AGCTGCGCGT CCTCCAGGCC 
GAGCTGAAGA TGGTCATCCG CCAGTCGCCG CAGTCGCCCA CCCAAGCGGT GCGCCTCTGT 
ATTGCCAACG ATTCTGGCTA CTTCCTGGAC ATCTCCATGT ACACGGAGCA GACGGAGCCG 
GAGACGGGAA TTATCAAGTT CAAGGCCTAT GGAGAGAAGC AGGGTTCCCT TCACGGCCAT 
CCCATTTCGA CGCCCTACAT GACCAAGGAC TTCCTGCAGC AGAAGCGCTT CCAGGCGCAG 
TCGAGCGGCA CCACGTACGT GTACGATGTG CCTGACATGT TCCGCCAGAT GACCGAGCGC 
CACTGGAAGG AGTTCTCCAA TGCCCGGCCC TCCGTGGACA TACGCATACC CGACAAGATT 
CTGATTGAAT GCAAGGAGCT GGTGCTGGAG GGCGACAGCC TCATTGAGAT GCAGCGTCTG 
CCGGGCGAAA ACAATTGCGG CATGGTGGCC TGGCGCATTG TCCTGGCCAC GCCGGAGTAC 
CCGAATGGGC GCGAGATCAT TGTGATAGCT AATGATCTGA CCTACCTGAT TGGCTCCTTT 
GGCATCAAAG AGGATGTGCT GTTCGCCAAG GCTTCCCAGT TGGCGCGCGA ACGGAAAGTG 
CCCAGGATCT ATATATCGGT GAACAGCGGT GCCCGCATTG GACTGGCCGA GGAGGTGAAA 
GCAATGTTCA AGATAGCATG GGAGGATCCC GAGGAGCCGG ACAAGGGCTT CAAGTACCTC 
TATCTGAGCA CCGAGGACTA TGCCCAGGTG GCCAATCTCA ATTCGGTGCG TGCCATTCTC 
ATCGAGGATG AGGGCGAGCA GCGCTACAAA ATCACCGACA TCATCGGCAA GGACGATGGC 
CTGGGCGTCG AAAATCTGCG CTATGCCGGC CTGATTGCCG GCGAAACGTC GCAGGCGTAC 
GAGGAGATTG TCACCATTGC GATGGTCACC TGCCGCACCA TCGGCATCGG CTCCTATGTG 
GTGCGTCTGG GCCAGCGTGT CATCCAGATC GACAACTCGC ACATTATACT CACGGGGTAT 
GCGGCCCTGA ATAAGCTGCT CGGTCGCAAG GTATACGCCT CAAACAACCA ATTGGGCGGC 
ACGCAGATCA TGTTCAACAA TGGCGTGACC CACAAAACTG AGGCCATCGA CTTGGACGGC 
GTGTATACCA TCCTCGACTG GCTCTCGTAC ATTCCCGCCC ACATTGGCTG CGATCTGCCC 
ATCGTTTCGC CCAGCGATCG TATCGATCGG CCTGTCGATT TTATGCCCAC CAAGTCGCCG 
TACGATCCGC GCTGCATGCT GGCCGGTCGT GTGAATCCCA CCAATGCAAA TGAGTGGGAG 
AATGGGTTCT TTGATCGTGA TTCCTGGAGC GAGATTATGG CCCCGTGGGC CAAGACCGTG 
GTCACGGGAC GTGCTCGACT GGGCGGTGTG CCTGTGGGTG TTATTGCCGT GGAGACGCGA 
ACTGTGGAGG TGGAAATGCC CGCGGATCCG GCCAACCTGG ATTCGGAAGC CAAGACACTG 
CAGCAGGCGG GTCAGGTGTG GTATCCCGAC TCATCCTACA AGACGGCGCA AGCCATTAAG 
GACTTTGGGC GCGAGGAGTT GCCTCTGATT GTGTTCGCCA ACTGGCGTGG CTTCTCTGGC 
GGCATGAAGG ACATGTACGA GCAGATCGTC AAGTTTGGGG CCTACATCGT TGACGGACTG 
CGGGAGTACA AGAAGCCGGT GCTCATCTAC CTGCCGCCCA ATGCCGAGCT GCGTGGCGGA 
GCCTGGGCCG TTCTGGACTC GTTGATCAAT CCGCGCTACA TGGAAACCTA TGCGGATCCG 
GAGGCCCGAG GTGGTGTCCT CGAACCCGAG GGTATTGTGG AAATCAAATA CAAAGAGAAG 
GACCTCATCA AGACGATAAA TCGTCTGGAT GCGACCACAA TTGCCCTAAA GAAGGAATTC 
GAAGAGGCAA CTGCAGCTGG TGATAACACG AAGGCCGAGC TGGTGGATGA GAAGATCAAG 
GCTCGTCTTG CGCAGCTAAT GCATGTCTAT CATACGGTGG CCGTGCACTT TGCCGACCTA 
CACGACACGC CCGAGCGCAT GCTGGAGAAG GAGTGCATCA GCGAGATTGT GCCATGGCGC 
GGCTCGCGAC GTTGGCTCTA CTGGCGCCTG CGCCGCCTCC TGCTGGAGGA TGCATACATC 
AAGAAGATCT TGCGGGCACA GGACAATCTC TCTGTCGGAC AGGCCAAGCA GATGCTCAGA 
CGCTGGCTAG TCGAAGAAAA GGGTGCCACA GAGGCATATC TGTGGGACAA GAATGAGGAA 
ATGGTGGCCT GGTATGAAGA GCAAACGAAT ACAGAATCTA TTGTCTCGCG CAATGTGAGT 
TCCGTGCGAA GGGATGCCAT CATCTCAACC ATTTCGAAAA TGCTCGAGGA CTGTCCCGAT 
GTAGCGCTGG ACGCTGTGGT TGGCCTCTGT CAAGGCCTGA CTCCCGTCAA TCGTGGAGTC 
GTCGTACGCA CACTGGCGCA AATGCAGCTG AACGAAGAGT CCTCTAGCAA TACCCAGGGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10832 ( XM_015184051.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039537.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10832), mRNA.

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CloneID	ODe22672
Clone ID Related Accession (Same CDS sequence)	XM_015184051.1
Accession Version	XM_015184051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAAAA ACAACTCCAG CGTGATTGCA GCTACAGCAC CACCAGTCAC AGCCACAAGC 
AGCTTGTCGT CGACCGCAAC GGGAGGAGCC ACAACAAAGC TGAACGAGAA GCGATTCCCC 
AAAGGAGCCA GCATCAAGAA GGTTCAGTTC TCCAGCGAAA GCCTTAAGGA GGTGGATATT 
GCCTTGTCCG ATCTGAGCTA TCACCAGAAC CACAACAACA ACACAACTGT CATCAAGAAC 
AAGAACAACA ATAATAGCAT TGTCGAAATC AAAAAGAAAT CATCAGTCGA AATGAGCGAG 
GAAACAAATG CAACGCCAAC AACGGATGCG GAGCGTCCAA GTTTCTTGGT GGGCGATGAG 
ATTGACGAAA AGGCAGCCGA AGCCGGCGAG GCTGTAGACG AGTTCCCCCA GAAGATGCAG 
AGCGCGTCCC GGCAGAATGG CGATATATCG GAGAAACGCA AGCGATTAAG ACCGAGCATG 
TCGCGTGGCA CTGGCCTGGG CCAGGACCGC CATCAGGATC GTGACTTTCA TATAGCCACC 
ACCGAGGAGT TTGTGAAGCG TTTCGGCGGT ACACGTGTCA TTAACCGCGT CCTCATTGCC 
AACAATGGCA TTGCGGCTGT CAAGTGCATG CGTTCCATTC GCCGATGGGC CTACGAGATG 
TTCAAGAACG AGCGGGCCAT CCGATTTGTG GTGATGGTCA CCCCGGAGGA CCTGAAGGCG 
AATGCCGAGT ACATCAAGAT GGCCGATCAC TATGTCCCCG TGCCCGGCGG TTCCAACAAC 
AACAACTATG CGAATGTTGA GCTCATTGTG GACATTGCAC TTCGCACACA AGTGCAGGCT 
GTCTGGGCTG GATGGGGTCA TGCCTCGGAG AACCCCAAGT TGCCGGAGCT GCTGCACAAG 
GAGGGTTTGG TGTTCCTCGG GCCACCGGAG CGCGCCATGT GGGCGCTGGG CGACAAGGTG 
GCCTCCTCGA TTGTGGCCCA AACGGCCGAA ATACCCACAC TACCATGGTC CGGCTCCGAC 
CTGAAAGCCC AGTACAGCGG CAAGAAGATC AAGATCACCA GCGACCTTTT TGCCCGCGGC 
TGTGTGACTA ATGTGGAGCA GGGTCTGGCG GCTGTCGCCA AGATTGGCTT TCCCGTGATG 
ATCAAGGCTT CGGAAGGCGG CGGCGGCAAG GGCATTCGTC GCGTGGACAC CACCGAAGAG 
TTCCCCGCCC TGTTCCGTCA GGTGCAGGCC GAGGTGCCCG GCTCCCCCAT CTTTGTGATG 
AAGCTGGCTC GCGGCGCACG CCACTTGGAA GTGCAGCTGC TGGCGGATCA GTACGGAAAT 
GCCATCAGTC TCTTTGGCCG CGATTGCTCC ATACAGCGTC GCCATCAAAA GATCATCGAG 
GAGGCCCCGG CCATTGTGGC CCAGCCGGAG GTGTTTGAGG ACATGGAAAA GGCGGCCGTG 
CGTCTGGCCA AGATGGTGGG CTATGTGAGC GCCGGCACCG TGGAGTACCT ATACGATCCG 
GAGGGCAGGT ACTTCTTCCT GGAGCTGAAC CCCCGTCTGC AGGTGGAGCA TCCCTGCACG 
GAGATGGTGG CCGATGTCAA TCTACCGGCC TGCCAGCTGC AGATCGGCAT GGGCATCCCC 
CTATACCGCC TCAAGGACAT TCGCCTGTTG TACGGTGAGT CGCCGTGGGG CTCCTCCATC 
ATTGACTTTG AGAATCCGCC GAACAAGCCC ATGCCCTCGG GCCATGTGAT CGCTGCGCGC 
ATCACCTCCG AGAATCCCGA CGAGGGCTTC AAGCCCAGCT CGGGCACAGT TCAGGAGCTC 
AACTTCCGCT CCAGCAAGAA CGTCTGGGGC TACTTCAGTG TGGCCGCCTC CGGGGGATTG 
CACGAGTTTG CCGACTCGCA GTTCGGTCAC TGCTTCTCCT GGGGCGAGAA CCGGCAGCAG 
GCGCGCGAGA ATCTCGTGAT TGCCCTCAAA GAGCTGTCGA TCCGTGGCGA TTTCCGCACC 
ACCGTCGAGT ACCTGATCAC GCTGCTCGAG ACGAATCGCT TCCTGGAGAA CACCATCGAC 
ACAGCCTGGC TGGATGCTCT GATTGCCGAG CGCGTGCAGT CCGAGAAGCC GGACATACTG 
CTGGGCGTGA TGTGCGGGGC CCTGCACATT GCCGATCGCC AGATCACCGA ATCCTTCTCC 
AGCTTCCAAA CGTCGCTGGA GAGGGGCCAG ATCCAGGCGG CCAACACCCT GACGAATGTG 
ATAGACGTGG AGCTGATCAA CGGAGGGATC CGGTACAAGG TTCAAGCGGC CAAGAGCGGC 
GCCAACTCGT ACTTCCTGCT GATGAACAGC TCCTTCAAGG AGATCGAGGT GCACCGCCTG 
TCCGACGGCG GCCTGCTGAT CTCCCTCGAG GGCGCCTCGT ACACCACCTA CATGAAGGAG 
GAGGTGGACC GCTACCGCAT CGTTATTGGC AACCAGACGT GCGTTTTCGA GAAGGAGAAC 
GATCCGTCGC TGCTGCGCAG CCCATCGGCG GGAAAGCTCA TCAATCTGAT TGTGGAGGAT 
GGGGCACATG TGGCCAAGGG CCAGCCCTTT GCCGAGATCG AGGTGATGAA GATGGTGATG 
ACCCTCACCT CGCAGGAGGC AGGCACGGTG ACCTTTGTGC GGCGTCCAGG CGCCGTCCTC 
GACCCGGGCT CCCTGCTGGG CCACCTGGAG CTCGATGATC CCTCGCTGGT GACCAAGGCG 
CAGCCCTGCA AGAGCCAGTT CCACCAGGCC GAGAACGCGC CCATTCCCGA GAAGCTGAAC 
CGCGTGCACA ACACGTACAA GAGCATTTTG GAGAACACCT TGGCCGGCTA CTGCCTGCCG 
GAGCCCTTCA ATGCCCAGCG GCTGCGCGAC ATCATCGAGA AGTTCATGCA GAGCCTGAGG 
GATCCATCGC TGCCGCTGCT GGAACTCCAG GAGGTGATTG CCTCCATCTC GGGCCGCATC 
CCCATCTCCG TAGAGAAGAA GATTCGCAAG CTGATGACCC TCTACGAGCG GAACATCACC 
AGTGTGTTGG CCCAGTTTCC CTCCCAGCAG ATCGCCAGCG TCATCGACAG CCACGCGGCC 
ACCTTGCAGA AGCGCGCCGA TCGCGACGTC TTCTTCCTGA CCACCCAGAG CATTGTGCAG 
CTGGTGCAGC GCTACCGGAA TGGCATTCGC GGCCGCATGA AGGCCGCCGT TCACGAACTG 
CTGCGCCAGT ACTACGACGT GGAGTCGCAG TTCCAGCACG GCCACTACGA CAAGTGCGTG 
GGCCTGGTGC GGGAGCACAA CAAGGATGAC ATGCTGACGG TGGTGAACAC GATATTCTCG 
CACTCGCAAG TGGCCAAGAA GAACTTGCTC GTTACGCTGC TAATCGACCA TCTGTGGGCC 
AACGAGCCGG GACTCACCGA CGAGCTGGCC AACACCCTCA GCGAGCTAAC GTCCCTCAAC 
AGGGCCGAGC ACTCGCGGGT GGCTCTGCGC TCTCGCCAGG TACTGATTGC CGCCCACCAG 
CCGGCATACG AGCTGCGCCA CAACCAAATG GAGTCCATAT TCCTGTCGGC CGTAGACATG 
TACGGCCACG ACTTCCATCC GGAGAATCTG CAGCGCCTGA TCCTCTCGGA GACCTCAATC 
TTTGACATTC TCCACGATTT CTTCTACCAC ACGAACCGAG CCGTGTGCAA CGCCGCCCTC 
GAGGTGTACG TGAGGCGCGC CTACACCTCC TATGAGCTGA CTTGCCTGCA GCACTTGGAG 
CTGTCCGGCG GCCTGCCGCT GGTGCATTTC CAGTTCCTGC TGCCCACGGC CCATCCGAAT 
CGTCTGTTCT CGCGCATGTC CTCGCCGGAG GGACTAGACC AGGCAGCCGC CGAGTCGATG 
GGCAGCTCCT TTGTCCGCAC CGGAGCGATT GCCGCCTTCG ATTCGTTCGA GCACTTTGAG 
ATGTACTCGG ACGAGATACT GGACCTTCTC GAGGACTTTG TCTCGCCCGC GATGGTCAAT 
GCCAAGGTGC TGGAGGCTGT GGAGGCAGCC GACTCCATCT CGGACGGCCG TCACAGCACC 
TCCATCAACG TCTCGCTGTC GGATCCCGTC ACCAGAGCCA ATGCCGCCGA GGAGGCCAAG 
TCCACGGAGC CCATTCACAT TGTGAGTGTG GCTGTGCGGG AGACCGGAGA TATGGACGAC 
TTGCAGATGG CCCAGATTTT CGGGAACTAC TGCAAGGAGC ACAACGAGGA GCTCTTCCAG 
CGCCGCATTC GGAGGATAAC GTTTGCCGCT TTGAAGAAGC GCCAGTTCCC CAAGTTCTTT 
ACATACCGCG CACGCGACAA GTTCGAGGAG GATCGCATTT ATCGGCATCT GGAGCCGGCC 
TCTGCCTTCC ATTTGGAGCT AAACCGCATG AAGACCTACA ACCTGGAGGC CCTGCCGACG 
GCCAACCAGA AGATGCATCT GTATTTGGGC AGGGCAAAGG TGTCCAAGGG ACAGGAGGTG 
ACCGACTATC GTTTCTTCAT CCGCTCGATC ATCCGCCACT CGGACCTCAT CACCAAGGAG 
GCCTCATTCG AGTACCTCCA GAACGAGGGC GAACGGGTGC TGCTGGAGGC CATGGACGAG 
CTGGAGGTGG CCTTCTCCCA CCCGCATGCC AAGCGCACCG ATTGCAATCA CATCTTTCTC 
AATTTCGTGC CCACCGTTAT TATGGACCCG GCCAAGATCG AGGAGTCCGT CACCAAGATG 
ATCATGCGCT ATGGCCCCCG CCTGTGGAAG CTGCGCGTCC TCCAGGCCGA GCTGAAGATG 
GTCATCCGCC AGTCGCCGCA GTCGCCCACC CAAGCGGTGC GCCTCTGTAT TGCCAACGAT 
TCTGGCTACT TCCTGGACAT CTCCATGTAC ACGGAGCAGA CGGAGCCGGA GACGGGAATT 
ATCAAGTTCA AGGCCTATGG AGAGAAGCAG GGTTCCCTTC ACGGCCATCC CATTTCGACG 
CCCTACATGA CCAAGGACTT CCTGCAGCAG AAGCGCTTCC AGGCGCAGTC GAGCGGCACC 
ACGTACGTGT ACGATGTGCC TGACATGTTC CGCCAGATGA CCGAGCGCCA CTGGAAGGAG 
TTCTCCAATG CCCGGCCCTC CGTGGACATA CGCATACCCG ACAAGATTCT GATTGAATGC 
AAGGAGCTGG TGCTGGAGGG CGACAGCCTC ATTGAGATGC AGCGTCTGCC GGGCGAAAAC 
AATTGCGGCA TGGTGGCCTG GCGCATTGTC CTGGCCACGC CGGAGTACCC GAATGGGCGC 
GAGATCATTG TGATAGCTAA TGATCTGACC TACCTGATTG GCTCCTTTGG CATCAAAGAG 
GATGTGCTGT TCGCCAAGGC TTCCCAGTTG GCGCGCGAAC GGAAAGTGCC CAGGATCTAT 
ATATCGGTGA ACAGCGGTGC CCGCATTGGA CTGGCCGAGG AGGTGAAAGC AATGTTCAAG 
ATAGCATGGG AGGATCCCGA GGAGCCGGAC AAGGGCTTCA AGTACCTCTA TCTGAGCACC 
GAGGACTATG CCCAGGTGGC CAATCTCAAT TCGGTGCGTG CCATTCTCAT CGAGGATGAG 
GGCGAGCAGC GCTACAAAAT CACCGACATC ATCGGCAAGG ACGATGGCCT GGGCGTCGAA 
AATCTGCGCT ATGCCGGCCT GATTGCCGGC GAAACGTCGC AGGCGTACGA GGAGATTGTC 
ACCATTGCGA TGGTCACCTG CCGCACCATC GGCATCGGCT CCTATGTGGT GCGTCTGGGC 
CAGCGTGTCA TCCAGATCGA CAACTCGCAC ATTATACTCA CGGGGTATGC GGCCCTGAAT 
AAGCTGCTCG GTCGCAAGGT ATACGCCTCA AACAACCAAT TGGGCGGCAC GCAGATCATG 
TTCAACAATG GCGTGACCCA CAAAACTGAG GCCATCGACT TGGACGGCGT GTATACCATC 
CTCGACTGGC TCTCGTACAT TCCCGCCCAC ATTGGCTGCG ATCTGCCCAT CGTTTCGCCC 
AGCGATCGTA TCGATCGGCC TGTCGATTTT ATGCCCACCA AGTCGCCGTA CGATCCGCGC 
TGCATGCTGG CCGGTCGTGT GAATCCCACC AATGCAAATG AGTGGGAGAA TGGGTTCTTT 
GATCGTGATT CCTGGAGCGA GATTATGGCC CCGTGGGCCA AGACCGTGGT CACGGGACGT 
GCTCGACTGG GCGGTGTGCC TGTGGGTGTT ATTGCCGTGG AGACGCGAAC TGTGGAGGTG 
GAAATGCCCG CGGATCCGGC CAACCTGGAT TCGGAAGCCA AGACACTGCA GCAGGCGGGT 
CAGGTGTGGT ATCCCGACTC ATCCTACAAG ACGGCGCAAG CCATTAAGGA CTTTGGGCGC 
GAGGAGTTGC CTCTGATTGT GTTCGCCAAC TGGCGTGGCT TCTCTGGCGG CATGAAGGAC 
ATGTACGAGC AGATCGTCAA GTTTGGGGCC TACATCGTTG ACGGACTGCG GGAGTACAAG 
AAGCCGGTGC TCATCTACCT GCCGCCCAAT GCCGAGCTGC GTGGCGGAGC CTGGGCCGTT 
CTGGACTCGT TGATCAATCC GCGCTACATG GAAACCTATG CGGATCCGGA GGCCCGAGGT 
GGTGTCCTCG AACCCGAGGG TATTGTGGAA ATCAAATACA AAGAGAAGGA CCTCATCAAG 
ACGATAAATC GTCTGGATGC GACCACAATT GCCCTAAAGA AGGAATTCGA AGAGGCAACT 
GCAGCTGGTG ATAACACGAA GGCCGAGCTG GTGGATGAGA AGATCAAGGC TCGTCTTGCG 
CAGCTAATGC ATGTCTATCA TACGGTGGCC GTGCACTTTG CCGACCTACA CGACACGCCC 
GAGCGCATGC TGGAGAAGGA GTGCATCAGC GAGATTGTGC CATGGCGCGG CTCGCGACGT 
TGGCTCTACT GGCGCCTGCG CCGCCTCCTG CTGGAGGATG CATACATCAA GAAGATCTTG 
CGGGCACAGG ACAATCTCTC TGTCGGACAG GCCAAGCAGA TGCTCAGACG CTGGCTAGTC 
GAAGAAAAGG GTGCCACAGA GGCATATCTG TGGGACAAGA ATGAGGAAAT GGTGGCCTGG 
TATGAAGAGC AAACGAATAC AGAATCTATT GTCTCGCGCA ATGTGAGTTC CGTGCGAAGG 
GATGCCATCA TCTCAACCAT TTCGAAAATG CTCGAGGACT GTCCCGATGT AGCGCTGGAC 
GCTGTGGTTG GCCTCTGTCA AGGCCTGACT CCCGTCAATC GTGGAGTCGT CGTACGCACA 
CTGGCGCAAA TGCAGCTGAA CGAAGAGTCC TCTAGCAATA CCCAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039537.1
CDS	198..7448
Translation	MEKNNSSVIAATAPPVTATSSLSSTATGGATTKLNEKRFPKGASIKKVQFSSESLKEVDIALSDLSYHQNHNNNTTVIKNKNNNNSIVEIKKKSSVEMSEETNATPTTDAERPSFLVGDEIDEKAAEAGEAVDEFPQKMQSASRQNGDISEKRKRLRPSMSRGTGLGQDRHQDRDFHIATTEEFVKRFGGTRVINRVLIANNGIAAVKCMRSIRRWAYEMFKNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGSNNNNYANVELIVDIALRTQVQAVWAGWGHASENPKLPELLHKEGLVFLGPPERAMWALGDKVASSIVAQTAEIPTLPWSGSDLKAQYSGKKIKITSDLFARGCVTNVEQGLAAVAKIGFPVMIKASEGGGGKGIRRVDTTEEFPALFRQVQAEVPGSPIFVMKLARGARHLEVQLLADQYGNAISLFGRDCSIQRRHQKIIEEAPAIVAQPEVFEDMEKAAVRLAKMVGYVSAGTVEYLYDPEGRYFFLELNPRLQVEHPCTEMVADVNLPACQLQIGMGIPLYRLKDIRLLYGESPWGSSIIDFENPPNKPMPSGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVAASGGLHEFADSQFGHCFSWGENRQQARENLVIALKELSIRGDFRTTVEYLITLLETNRFLENTIDTAWLDALIAERVQSEKPDILLGVMCGALHIADRQITESFSSFQTSLERGQIQAANTLTNVIDVELINGGIRYKVQAAKSGANSYFLLMNSSFKEIEVHRLSDGGLLISLEGASYTTYMKEEVDRYRIVIGNQTCVFEKENDPSLLRSPSAGKLINLIVEDGAHVAKGQPFAEIEVMKMVMTLTSQEAGTVTFVRRPGAVLDPGSLLGHLELDDPSLVTKAQPCKSQFHQAENAPIPEKLNRVHNTYKSILENTLAGYCLPEPFNAQRLRDIIEKFMQSLRDPSLPLLELQEVIASISGRIPISVEKKIRKLMTLYERNITSVLAQFPSQQIASVIDSHAATLQKRADRDVFFLTTQSIVQLVQRYRNGIRGRMKAAVHELLRQYYDVESQFQHGHYDKCVGLVREHNKDDMLTVVNTIFSHSQVAKKNLLVTLLIDHLWANEPGLTDELANTLSELTSLNRAEHSRVALRSRQVLIAAHQPAYELRHNQMESIFLSAVDMYGHDFHPENLQRLILSETSIFDILHDFFYHTNRAVCNAALEVYVRRAYTSYELTCLQHLELSGGLPLVHFQFLLPTAHPNRLFSRMSSPEGLDQAAAESMGSSFVRTGAIAAFDSFEHFEMYSDEILDLLEDFVSPAMVNAKVLEAVEAADSISDGRHSTSINVSLSDPVTRANAAEEAKSTEPIHIVSVAVRETGDMDDLQMAQIFGNYCKEHNEELFQRRIRRITFAALKKRQFPKFFTYRARDKFEEDRIYRHLEPASAFHLELNRMKTYNLEALPTANQKMHLYLGRAKVSKGQEVTDYRFFIRSIIRHSDLITKEASFEYLQNEGERVLLEAMDELEVAFSHPHAKRTDCNHIFLNFVPTVIMDPAKIEESVTKMIMRYGPRLWKLRVLQAELKMVIRQSPQSPTQAVRLCIANDSGYFLDISMYTEQTEPETGIIKFKAYGEKQGSLHGHPISTPYMTKDFLQQKRFQAQSSGTTYVYDVPDMFRQMTERHWKEFSNARPSVDIRIPDKILIECKELVLEGDSLIEMQRLPGENNCGMVAWRIVLATPEYPNGREIIVIANDLTYLIGSFGIKEDVLFAKASQLARERKVPRIYISVNSGARIGLAEEVKAMFKIAWEDPEEPDKGFKYLYLSTEDYAQVANLNSVRAILIEDEGEQRYKITDIIGKDDGLGVENLRYAGLIAGETSQAYEEIVTIAMVTCRTIGIGSYVVRLGQRVIQIDNSHIILTGYAALNKLLGRKVYASNNQLGGTQIMFNNGVTHKTEAIDLDGVYTILDWLSYIPAHIGCDLPIVSPSDRIDRPVDFMPTKSPYDPRCMLAGRVNPTNANEWENGFFDRDSWSEIMAPWAKTVVTGRARLGGVPVGVIAVETRTVEVEMPADPANLDSEAKTLQQAGQVWYPDSSYKTAQAIKDFGREELPLIVFANWRGFSGGMKDMYEQIVKFGAYIVDGLREYKKPVLIYLPPNAELRGGAWAVLDSLINPRYMETYADPEARGGVLEPEGIVEIKYKEKDLIKTINRLDATTIALKKEFEEATAAGDNTKAELVDEKIKARLAQLMHVYHTVAVHFADLHDTPERMLEKECISEIVPWRGSRRWLYWRLRRLLLEDAYIKKILRAQDNLSVGQAKQMLRRWLVEEKGATEAYLWDKNEEMVAWYEEQTNTESIVSRNVSSVRRDAIISTISKMLEDCPDVALDAVVGLCQGLTPVNRGVVVRTLAQMQLNEESSSNTQG

Target ORF information:

RefSeq Version	XM_015184051.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA10832), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184051.1

ORF Insert Sequence:

ATGGAGAAAA ACAACTCCAG CGTGATTGCA GCTACAGCAC CACCAGTCAC AGCCACAAGC 
AGCTTGTCGT CGACCGCAAC GGGAGGAGCC ACAACAAAGC TGAACGAGAA GCGATTCCCC 
AAAGGAGCCA GCATCAAGAA GGTTCAGTTC TCCAGCGAAA GCCTTAAGGA GGTGGATATT 
GCCTTGTCCG ATCTGAGCTA TCACCAGAAC CACAACAACA ACACAACTGT CATCAAGAAC 
AAGAACAACA ATAATAGCAT TGTCGAAATC AAAAAGAAAT CATCAGTCGA AATGAGCGAG 
GAAACAAATG CAACGCCAAC AACGGATGCG GAGCGTCCAA GTTTCTTGGT GGGCGATGAG 
ATTGACGAAA AGGCAGCCGA AGCCGGCGAG GCTGTAGACG AGTTCCCCCA GAAGATGCAG 
AGCGCGTCCC GGCAGAATGG CGATATATCG GAGAAACGCA AGCGATTAAG ACCGAGCATG 
TCGCGTGGCA CTGGCCTGGG CCAGGACCGC CATCAGGATC GTGACTTTCA TATAGCCACC 
ACCGAGGAGT TTGTGAAGCG TTTCGGCGGT ACACGTGTCA TTAACCGCGT CCTCATTGCC 
AACAATGGCA TTGCGGCTGT CAAGTGCATG CGTTCCATTC GCCGATGGGC CTACGAGATG 
TTCAAGAACG AGCGGGCCAT CCGATTTGTG GTGATGGTCA CCCCGGAGGA CCTGAAGGCG 
AATGCCGAGT ACATCAAGAT GGCCGATCAC TATGTCCCCG TGCCCGGCGG TTCCAACAAC 
AACAACTATG CGAATGTTGA GCTCATTGTG GACATTGCAC TTCGCACACA AGTGCAGGCT 
GTCTGGGCTG GATGGGGTCA TGCCTCGGAG AACCCCAAGT TGCCGGAGCT GCTGCACAAG 
GAGGGTTTGG TGTTCCTCGG GCCACCGGAG CGCGCCATGT GGGCGCTGGG CGACAAGGTG 
GCCTCCTCGA TTGTGGCCCA AACGGCCGAA ATACCCACAC TACCATGGTC CGGCTCCGAC 
CTGAAAGCCC AGTACAGCGG CAAGAAGATC AAGATCACCA GCGACCTTTT TGCCCGCGGC 
TGTGTGACTA ATGTGGAGCA GGGTCTGGCG GCTGTCGCCA AGATTGGCTT TCCCGTGATG 
ATCAAGGCTT CGGAAGGCGG CGGCGGCAAG GGCATTCGTC GCGTGGACAC CACCGAAGAG 
TTCCCCGCCC TGTTCCGTCA GGTGCAGGCC GAGGTGCCCG GCTCCCCCAT CTTTGTGATG 
AAGCTGGCTC GCGGCGCACG CCACTTGGAA GTGCAGCTGC TGGCGGATCA GTACGGAAAT 
GCCATCAGTC TCTTTGGCCG CGATTGCTCC ATACAGCGTC GCCATCAAAA GATCATCGAG 
GAGGCCCCGG CCATTGTGGC CCAGCCGGAG GTGTTTGAGG ACATGGAAAA GGCGGCCGTG 
CGTCTGGCCA AGATGGTGGG CTATGTGAGC GCCGGCACCG TGGAGTACCT ATACGATCCG 
GAGGGCAGGT ACTTCTTCCT GGAGCTGAAC CCCCGTCTGC AGGTGGAGCA TCCCTGCACG 
GAGATGGTGG CCGATGTCAA TCTACCGGCC TGCCAGCTGC AGATCGGCAT GGGCATCCCC 
CTATACCGCC TCAAGGACAT TCGCCTGTTG TACGGTGAGT CGCCGTGGGG CTCCTCCATC 
ATTGACTTTG AGAATCCGCC GAACAAGCCC ATGCCCTCGG GCCATGTGAT CGCTGCGCGC 
ATCACCTCCG AGAATCCCGA CGAGGGCTTC AAGCCCAGCT CGGGCACAGT TCAGGAGCTC 
AACTTCCGCT CCAGCAAGAA CGTCTGGGGC TACTTCAGTG TGGCCGCCTC CGGGGGATTG 
CACGAGTTTG CCGACTCGCA GTTCGGTCAC TGCTTCTCCT GGGGCGAGAA CCGGCAGCAG 
GCGCGCGAGA ATCTCGTGAT TGCCCTCAAA GAGCTGTCGA TCCGTGGCGA TTTCCGCACC 
ACCGTCGAGT ACCTGATCAC GCTGCTCGAG ACGAATCGCT TCCTGGAGAA CACCATCGAC 
ACAGCCTGGC TGGATGCTCT GATTGCCGAG CGCGTGCAGT CCGAGAAGCC GGACATACTG 
CTGGGCGTGA TGTGCGGGGC CCTGCACATT GCCGATCGCC AGATCACCGA ATCCTTCTCC 
AGCTTCCAAA CGTCGCTGGA GAGGGGCCAG ATCCAGGCGG CCAACACCCT GACGAATGTG 
ATAGACGTGG AGCTGATCAA CGGAGGGATC CGGTACAAGG TTCAAGCGGC CAAGAGCGGC 
GCCAACTCGT ACTTCCTGCT GATGAACAGC TCCTTCAAGG AGATCGAGGT GCACCGCCTG 
TCCGACGGCG GCCTGCTGAT CTCCCTCGAG GGCGCCTCGT ACACCACCTA CATGAAGGAG 
GAGGTGGACC GCTACCGCAT CGTTATTGGC AACCAGACGT GCGTTTTCGA GAAGGAGAAC 
GATCCGTCGC TGCTGCGCAG CCCATCGGCG GGAAAGCTCA TCAATCTGAT TGTGGAGGAT 
GGGGCACATG TGGCCAAGGG CCAGCCCTTT GCCGAGATCG AGGTGATGAA GATGGTGATG 
ACCCTCACCT CGCAGGAGGC AGGCACGGTG ACCTTTGTGC GGCGTCCAGG CGCCGTCCTC 
GACCCGGGCT CCCTGCTGGG CCACCTGGAG CTCGATGATC CCTCGCTGGT GACCAAGGCG 
CAGCCCTGCA AGAGCCAGTT CCACCAGGCC GAGAACGCGC CCATTCCCGA GAAGCTGAAC 
CGCGTGCACA ACACGTACAA GAGCATTTTG GAGAACACCT TGGCCGGCTA CTGCCTGCCG 
GAGCCCTTCA ATGCCCAGCG GCTGCGCGAC ATCATCGAGA AGTTCATGCA GAGCCTGAGG 
GATCCATCGC TGCCGCTGCT GGAACTCCAG GAGGTGATTG CCTCCATCTC GGGCCGCATC 
CCCATCTCCG TAGAGAAGAA GATTCGCAAG CTGATGACCC TCTACGAGCG GAACATCACC 
AGTGTGTTGG CCCAGTTTCC CTCCCAGCAG ATCGCCAGCG TCATCGACAG CCACGCGGCC 
ACCTTGCAGA AGCGCGCCGA TCGCGACGTC TTCTTCCTGA CCACCCAGAG CATTGTGCAG 
CTGGTGCAGC GCTACCGGAA TGGCATTCGC GGCCGCATGA AGGCCGCCGT TCACGAACTG 
CTGCGCCAGT ACTACGACGT GGAGTCGCAG TTCCAGCACG GCCACTACGA CAAGTGCGTG 
GGCCTGGTGC GGGAGCACAA CAAGGATGAC ATGCTGACGG TGGTGAACAC GATATTCTCG 
CACTCGCAAG TGGCCAAGAA GAACTTGCTC GTTACGCTGC TAATCGACCA TCTGTGGGCC 
AACGAGCCGG GACTCACCGA CGAGCTGGCC AACACCCTCA GCGAGCTAAC GTCCCTCAAC 
AGGGCCGAGC ACTCGCGGGT GGCTCTGCGC TCTCGCCAGG TACTGATTGC CGCCCACCAG 
CCGGCATACG AGCTGCGCCA CAACCAAATG GAGTCCATAT TCCTGTCGGC CGTAGACATG 
TACGGCCACG ACTTCCATCC GGAGAATCTG CAGCGCCTGA TCCTCTCGGA GACCTCAATC 
TTTGACATTC TCCACGATTT CTTCTACCAC ACGAACCGAG CCGTGTGCAA CGCCGCCCTC 
GAGGTGTACG TGAGGCGCGC CTACACCTCC TATGAGCTGA CTTGCCTGCA GCACTTGGAG 
CTGTCCGGCG GCCTGCCGCT GGTGCATTTC CAGTTCCTGC TGCCCACGGC CCATCCGAAT 
CGTCTGTTCT CGCGCATGTC CTCGCCGGAG GGACTAGACC AGGCAGCCGC CGAGTCGATG 
GGCAGCTCCT TTGTCCGCAC CGGAGCGATT GCCGCCTTCG ATTCGTTCGA GCACTTTGAG 
ATGTACTCGG ACGAGATACT GGACCTTCTC GAGGACTTTG TCTCGCCCGC GATGGTCAAT 
GCCAAGGTGC TGGAGGCTGT GGAGGCAGCC GACTCCATCT CGGACGGCCG TCACAGCACC 
TCCATCAACG TCTCGCTGTC GGATCCCGTC ACCAGAGCCA ATGCCGCCGA GGAGGCCAAG 
TCCACGGAGC CCATTCACAT TGTGAGTGTG GCTGTGCGGG AGACCGGAGA TATGGACGAC 
TTGCAGATGG CCCAGATTTT CGGGAACTAC TGCAAGGAGC ACAACGAGGA GCTCTTCCAG 
CGCCGCATTC GGAGGATAAC GTTTGCCGCT TTGAAGAAGC GCCAGTTCCC CAAGTTCTTT 
ACATACCGCG CACGCGACAA GTTCGAGGAG GATCGCATTT ATCGGCATCT GGAGCCGGCC 
TCTGCCTTCC ATTTGGAGCT AAACCGCATG AAGACCTACA ACCTGGAGGC CCTGCCGACG 
GCCAACCAGA AGATGCATCT GTATTTGGGC AGGGCAAAGG TGTCCAAGGG ACAGGAGGTG 
ACCGACTATC GTTTCTTCAT CCGCTCGATC ATCCGCCACT CGGACCTCAT CACCAAGGAG 
GCCTCATTCG AGTACCTCCA GAACGAGGGC GAACGGGTGC TGCTGGAGGC CATGGACGAG 
CTGGAGGTGG CCTTCTCCCA CCCGCATGCC AAGCGCACCG ATTGCAATCA CATCTTTCTC 
AATTTCGTGC CCACCGTTAT TATGGACCCG GCCAAGATCG AGGAGTCCGT CACCAAGATG 
ATCATGCGCT ATGGCCCCCG CCTGTGGAAG CTGCGCGTCC TCCAGGCCGA GCTGAAGATG 
GTCATCCGCC AGTCGCCGCA GTCGCCCACC CAAGCGGTGC GCCTCTGTAT TGCCAACGAT 
TCTGGCTACT TCCTGGACAT CTCCATGTAC ACGGAGCAGA CGGAGCCGGA GACGGGAATT 
ATCAAGTTCA AGGCCTATGG AGAGAAGCAG GGTTCCCTTC ACGGCCATCC CATTTCGACG 
CCCTACATGA CCAAGGACTT CCTGCAGCAG AAGCGCTTCC AGGCGCAGTC GAGCGGCACC 
ACGTACGTGT ACGATGTGCC TGACATGTTC CGCCAGATGA CCGAGCGCCA CTGGAAGGAG 
TTCTCCAATG CCCGGCCCTC CGTGGACATA CGCATACCCG ACAAGATTCT GATTGAATGC 
AAGGAGCTGG TGCTGGAGGG CGACAGCCTC ATTGAGATGC AGCGTCTGCC GGGCGAAAAC 
AATTGCGGCA TGGTGGCCTG GCGCATTGTC CTGGCCACGC CGGAGTACCC GAATGGGCGC 
GAGATCATTG TGATAGCTAA TGATCTGACC TACCTGATTG GCTCCTTTGG CATCAAAGAG 
GATGTGCTGT TCGCCAAGGC TTCCCAGTTG GCGCGCGAAC GGAAAGTGCC CAGGATCTAT 
ATATCGGTGA ACAGCGGTGC CCGCATTGGA CTGGCCGAGG AGGTGAAAGC AATGTTCAAG 
ATAGCATGGG AGGATCCCGA GGAGCCGGAC AAGGGCTTCA AGTACCTCTA TCTGAGCACC 
GAGGACTATG CCCAGGTGGC CAATCTCAAT TCGGTGCGTG CCATTCTCAT CGAGGATGAG 
GGCGAGCAGC GCTACAAAAT CACCGACATC ATCGGCAAGG ACGATGGCCT GGGCGTCGAA 
AATCTGCGCT ATGCCGGCCT GATTGCCGGC GAAACGTCGC AGGCGTACGA GGAGATTGTC 
ACCATTGCGA TGGTCACCTG CCGCACCATC GGCATCGGCT CCTATGTGGT GCGTCTGGGC 
CAGCGTGTCA TCCAGATCGA CAACTCGCAC ATTATACTCA CGGGGTATGC GGCCCTGAAT 
AAGCTGCTCG GTCGCAAGGT ATACGCCTCA AACAACCAAT TGGGCGGCAC GCAGATCATG 
TTCAACAATG GCGTGACCCA CAAAACTGAG GCCATCGACT TGGACGGCGT GTATACCATC 
CTCGACTGGC TCTCGTACAT TCCCGCCCAC ATTGGCTGCG ATCTGCCCAT CGTTTCGCCC 
AGCGATCGTA TCGATCGGCC TGTCGATTTT ATGCCCACCA AGTCGCCGTA CGATCCGCGC 
TGCATGCTGG CCGGTCGTGT GAATCCCACC AATGCAAATG AGTGGGAGAA TGGGTTCTTT 
GATCGTGATT CCTGGAGCGA GATTATGGCC CCGTGGGCCA AGACCGTGGT CACGGGACGT 
GCTCGACTGG GCGGTGTGCC TGTGGGTGTT ATTGCCGTGG AGACGCGAAC TGTGGAGGTG 
GAAATGCCCG CGGATCCGGC CAACCTGGAT TCGGAAGCCA AGACACTGCA GCAGGCGGGT 
CAGGTGTGGT ATCCCGACTC ATCCTACAAG ACGGCGCAAG CCATTAAGGA CTTTGGGCGC 
GAGGAGTTGC CTCTGATTGT GTTCGCCAAC TGGCGTGGCT TCTCTGGCGG CATGAAGGAC 
ATGTACGAGC AGATCGTCAA GTTTGGGGCC TACATCGTTG ACGGACTGCG GGAGTACAAG 
AAGCCGGTGC TCATCTACCT GCCGCCCAAT GCCGAGCTGC GTGGCGGAGC CTGGGCCGTT 
CTGGACTCGT TGATCAATCC GCGCTACATG GAAACCTATG CGGATCCGGA GGCCCGAGGT 
GGTGTCCTCG AACCCGAGGG TATTGTGGAA ATCAAATACA AAGAGAAGGA CCTCATCAAG 
ACGATAAATC GTCTGGATGC GACCACAATT GCCCTAAAGA AGGAATTCGA AGAGGCAACT 
GCAGCTGGTG ATAACACGAA GGCCGAGCTG GTGGATGAGA AGATCAAGGC TCGTCTTGCG 
CAGCTAATGC ATGTCTATCA TACGGTGGCC GTGCACTTTG CCGACCTACA CGACACGCCC 
GAGCGCATGC TGGAGAAGGA GTGCATCAGC GAGATTGTGC CATGGCGCGG CTCGCGACGT 
TGGCTCTACT GGCGCCTGCG CCGCCTCCTG CTGGAGGATG CATACATCAA GAAGATCTTG 
CGGGCACAGG ACAATCTCTC TGTCGGACAG GCCAAGCAGA TGCTCAGACG CTGGCTAGTC 
GAAGAAAAGG GTGCCACAGA GGCATATCTG TGGGACAAGA ATGAGGAAAT GGTGGCCTGG 
TATGAAGAGC AAACGAATAC AGAATCTATT GTCTCGCGCA ATGTGAGTTC CGTGCGAAGG 
GATGCCATCA TCTCAACCAT TTCGAAAATG CTCGAGGACT GTCCCGATGT AGCGCTGGAC 
GCTGTGGTTG GCCTCTGTCA AGGCCTGACT CCCGTCAATC GTGGAGTCGT CGTACGCACA 
CTGGCGCAAA TGCAGCTGAA CGAAGAGTCC TCTAGCAATA CCCAGGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10832 ( XM_015184052.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015039538.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA10832), partial mRNA.

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CloneID	ODe22673
Clone ID Related Accession (Same CDS sequence)	XM_015184052.1
Accession Version	XM_015184052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTTAACCA GGACGCTGTT TAACCGCTGC TGCAACGGAC GCTGCATCAG AATGGCGGCT 
CCACACGTGA GGCACAACTC CAGCGTGATT GCAGCTACAG CACCACCAGT CACAGCCACA 
AGCAGCTTGT CGTCGACCGC AACGGGAGGA GCCACAACAA AGCTGAACGA GAAGCGATTC 
CCCAAAGGAG CCAGCATCAA GAAGGTTCAG TTCTCCAGCG AAAGCCTTAA GGAGGTGGAT 
ATTGCCTTGT CCGATCTGAG CTATCACCAG AACCACAACA ACAACACAAC TGTCATCAAG 
AACAAGAACA ACAATAATAG CATTGTCGAA ATCAAAAAGA AATCATCAGT CGAAATGAGC 
GAGGAAACAA ATGCAACGCC AACAACGGAT GCGGAGCGTC CAAGTTTCTT GGTGGGCGAT 
GAGATTGACG AAAAGGCAGC CGAAGCCGGC GAGGCTGTAG ACGAGTTCCC CCAGAAGATG 
CAGAGCGCGT CCCGGCAGAA TGGCGATATA TCGGAGAAAC GCAAGCGATT AAGACCGAGC 
ATGTCGCGTG GCACTGGCCT GGGCCAGGAC CGCCATCAGG ATCGTGACTT TCATATAGCC 
ACCACCGAGG AGTTTGTGAA GCGTTTCGGC GGTACACGTG TCATTAACCG CGTCCTCATT 
GCCAACAATG GCATTGCGGC TGTCAAGTGC ATGCGTTCCA TTCGCCGATG GGCCTACGAG 
ATGTTCAAGA ACGAGCGGGC CATCCGATTT GTGGTGATGG TCACCCCGGA GGACCTGAAG 
GCGAATGCCG AGTACATCAA GATGGCCGAT CACTATGTCC CCGTGCCCGG CGGTTCCAAC 
AACAACAACT ATGCGAATGT TGAGCTCATT GTGGACATTG CACTTCGCAC ACAAGTGCAG 
GCTGTCTGGG CTGGATGGGG TCATGCCTCG GAGAACCCCA AGTTGCCGGA GCTGCTGCAC 
AAGGAGGGTT TGGTGTTCCT CGGGCCACCG GAGCGCGCCA TGTGGGCGCT GGGCGACAAG 
GTGGCCTCCT CGATTGTGGC CCAAACGGCC GAAATACCCA CACTACCATG GTCCGGCTCC 
GACCTGAAAG CCCAGTACAG CGGCAAGAAG ATCAAGATCA CCAGCGACCT TTTTGCCCGC 
GGCTGTGTGA CTAATGTGGA GCAGGGTCTG GCGGCTGTCG CCAAGATTGG CTTTCCCGTG 
ATGATCAAGG CTTCGGAAGG CGGCGGCGGC AAGGGCATTC GTCGCGTGGA CACCACCGAA 
GAGTTCCCCG CCCTGTTCCG TCAGGTGCAG GCCGAGGTGC CCGGCTCCCC CATCTTTGTG 
ATGAAGCTGG CTCGCGGCGC ACGCCACTTG GAAGTGCAGC TGCTGGCGGA TCAGTACGGA 
AATGCCATCA GTCTCTTTGG CCGCGATTGC TCCATACAGC GTCGCCATCA AAAGATCATC 
GAGGAGGCCC CGGCCATTGT GGCCCAGCCG GAGGTGTTTG AGGACATGGA AAAGGCGGCC 
GTGCGTCTGG CCAAGATGGT GGGCTATGTG AGCGCCGGCA CCGTGGAGTA CCTATACGAT 
CCGGAGGGCA GGTACTTCTT CCTGGAGCTG AACCCCCGTC TGCAGGTGGA GCATCCCTGC 
ACGGAGATGG TGGCCGATGT CAATCTACCG GCCTGCCAGC TGCAGATCGG CATGGGCATC 
CCCCTATACC GCCTCAAGGA CATTCGCCTG TTGTACGGTG AGTCGCCGTG GGGCTCCTCC 
ATCATTGACT TTGAGAATCC GCCGAACAAG CCCATGCCCT CGGGCCATGT GATCGCTGCG 
CGCATCACCT CCGAGAATCC CGACGAGGGC TTCAAGCCCA GCTCGGGCAC AGTTCAGGAG 
CTCAACTTCC GCTCCAGCAA GAACGTCTGG GGCTACTTCA GTGTGGCCGC CTCCGGGGGA 
TTGCACGAGT TTGCCGACTC GCAGTTCGGT CACTGCTTCT CCTGGGGCGA GAACCGGCAG 
CAGGCGCGCG AGAATCTCGT GATTGCCCTC AAAGAGCTGT CGATCCGTGG CGATTTCCGC 
ACCACCGTCG AGTACCTGAT CACGCTGCTC GAGACGAATC GCTTCCTGGA GAACACCATC 
GACACAGCCT GGCTGGATGC TCTGATTGCC GAGCGCGTGC AGTCCGAGAA GCCGGACATA 
CTGCTGGGCG TGATGTGCGG GGCCCTGCAC ATTGCCGATC GCCAGATCAC CGAATCCTTC 
TCCAGCTTCC AAACGTCGCT GGAGAGGGGC CAGATCCAGG CGGCCAACAC CCTGACGAAT 
GTGATAGACG TGGAGCTGAT CAACGGAGGG ATCCGGTACA AGGTTCAAGC GGCCAAGAGC 
GGCGCCAACT CGTACTTCCT GCTGATGAAC AGCTCCTTCA AGGAGATCGA GGTGCACCGC 
CTGTCCGACG GCGGCCTGCT GATCTCCCTC GAGGGCGCCT CGTACACCAC CTACATGAAG 
GAGGAGGTGG ACCGCTACCG CATCGTTATT GGCAACCAGA CGTGCGTTTT CGAGAAGGAG 
AACGATCCGT CGCTGCTGCG CAGCCCATCG GCGGGAAAGC TCATCAATCT GATTGTGGAG 
GATGGGGCAC ATGTGGCCAA GGGCCAGCCC TTTGCCGAGA TCGAGGTGAT GAAGATGGTG 
ATGACCCTCA CCTCGCAGGA GGCAGGCACG GTGACCTTTG TGCGGCGTCC AGGCGCCGTC 
CTCGACCCGG GCTCCCTGCT GGGCCACCTG GAGCTCGATG ATCCCTCGCT GGTGACCAAG 
GCGCAGCCCT GCAAGAGCCA GTTCCACCAG GCCGAGAACG CGCCCATTCC CGAGAAGCTG 
AACCGCGTGC ACAACACGTA CAAGAGCATT TTGGAGAACA CCTTGGCCGG CTACTGCCTG 
CCGGAGCCCT TCAATGCCCA GCGGCTGCGC GACATCATCG AGAAGTTCAT GCAGAGCCTG 
AGGGATCCAT CGCTGCCGCT GCTGGAACTC CAGGAGGTGA TTGCCTCCAT CTCGGGCCGC 
ATCCCCATCT CCGTAGAGAA GAAGATTCGC AAGCTGATGA CCCTCTACGA GCGGAACATC 
ACCAGTGTGT TGGCCCAGTT TCCCTCCCAG CAGATCGCCA GCGTCATCGA CAGCCACGCG 
GCCACCTTGC AGAAGCGCGC CGATCGCGAC GTCTTCTTCC TGACCACCCA GAGCATTGTG 
CAGCTGGTGC AGCGCTACCG GAATGGCATT CGCGGCCGCA TGAAGGCCGC CGTTCACGAA 
CTGCTGCGCC AGTACTACGA CGTGGAGTCG CAGTTCCAGC ACGGCCACTA CGACAAGTGC 
GTGGGCCTGG TGCGGGAGCA CAACAAGGAT GACATGCTGA CGGTGGTGAA CACGATATTC 
TCGCACTCGC AAGTGGCCAA GAAGAACTTG CTCGTTACGC TGCTAATCGA CCATCTGTGG 
GCCAACGAGC CGGGACTCAC CGACGAGCTG GCCAACACCC TCAGCGAGCT AACGTCCCTC 
AACAGGGCCG AGCACTCGCG GGTGGCTCTG CGCTCTCGCC AGGTACTGAT TGCCGCCCAC 
CAGCCGGCAT ACGAGCTGCG CCACAACCAA ATGGAGTCCA TATTCCTGTC GGCCGTAGAC 
ATGTACGGCC ACGACTTCCA TCCGGAGAAT CTGCAGCGCC TGATCCTCTC GGAGACCTCA 
ATCTTTGACA TTCTCCACGA TTTCTTCTAC CACACGAACC GAGCCGTGTG CAACGCCGCC 
CTCGAGGTGT ACGTGAGGCG CGCCTACACC TCCTATGAGC TGACTTGCCT GCAGCACTTG 
GAGCTGTCCG GCGGCCTGCC GCTGGTGCAT TTCCAGTTCC TGCTGCCCAC GGCCCATCCG 
AATCGTCTGT TCTCGCGCAT GTCCTCGCCG GAGGGACTAG ACCAGGCAGC CGCCGAGTCG 
ATGGGCAGCT CCTTTGTCCG CACCGGAGCG ATTGCCGCCT TCGATTCGTT CGAGCACTTT 
GAGATGTACT CGGACGAGAT ACTGGACCTT CTCGAGGACT TTGTCTCGCC CGCGATGGTC 
AATGCCAAGG TGCTGGAGGC TGTGGAGGCA GCCGACTCCA TCTCGGACGG CCGTCACAGC 
ACCTCCATCA ACGTCTCGCT GTCGGATCCC GTCACCAGAG CCAATGCCGC CGAGGAGGCC 
AAGTCCACGG AGCCCATTCA CATTGTGAGT GTGGCTGTGC GGGAGACCGG AGATATGGAC 
GACTTGCAGA TGGCCCAGAT TTTCGGGAAC TACTGCAAGG AGCACAACGA GGAGCTCTTC 
CAGCGCCGCA TTCGGAGGAT AACGTTTGCC GCTTTGAAGA AGCGCCAGTT CCCCAAGTTC 
TTTACATACC GCGCACGCGA CAAGTTCGAG GAGGATCGCA TTTATCGGCA TCTGGAGCCG 
GCCTCTGCCT TCCATTTGGA GCTAAACCGC ATGAAGACCT ACAACCTGGA GGCCCTGCCG 
ACGGCCAACC AGAAGATGCA TCTGTATTTG GGCAGGGCAA AGGTGTCCAA GGGACAGGAG 
GTGACCGACT ATCGTTTCTT CATCCGCTCG ATCATCCGCC ACTCGGACCT CATCACCAAG 
GAGGCCTCAT TCGAGTACCT CCAGAACGAG GGCGAACGGG TGCTGCTGGA GGCCATGGAC 
GAGCTGGAGG TGGCCTTCTC CCACCCGCAT GCCAAGCGCA CCGATTGCAA TCACATCTTT 
CTCAATTTCG TGCCCACCGT TATTATGGAC CCGGCCAAGA TCGAGGAGTC CGTCACCAAG 
ATGATCATGC GCTATGGCCC CCGCCTGTGG AAGCTGCGCG TCCTCCAGGC CGAGCTGAAG 
ATGGTCATCC GCCAGTCGCC GCAGTCGCCC ACCCAAGCGG TGCGCCTCTG TATTGCCAAC 
GATTCTGGCT ACTTCCTGGA CATCTCCATG TACACGGAGC AGACGGAGCC GGAGACGGGA 
ATTATCAAGT TCAAGGCCTA TGGAGAGAAG CAGGGTTCCC TTCACGGCCA TCCCATTTCG 
ACGCCCTACA TGACCAAGGA CTTCCTGCAG CAGAAGCGCT TCCAGGCGCA GTCGAGCGGC 
ACCACGTACG TGTACGATGT GCCTGACATG TTCCGCCAGA TGACCGAGCG CCACTGGAAG 
GAGTTCTCCA ATGCCCGGCC CTCCGTGGAC ATACGCATAC CCGACAAGAT TCTGATTGAA 
TGCAAGGAGC TGGTGCTGGA GGGCGACAGC CTCATTGAGA TGCAGCGTCT GCCGGGCGAA 
AACAATTGCG GCATGGTGGC CTGGCGCATT GTCCTGGCCA CGCCGGAGTA CCCGAATGGG 
CGCGAGATCA TTGTGATAGC TAATGATCTG ACCTACCTGA TTGGCTCCTT TGGCATCAAA 
GAGGATGTGC TGTTCGCCAA GGCTTCCCAG TTGGCGCGCG AACGGAAAGT GCCCAGGATC 
TATATATCGG TGAACAGCGG TGCCCGCATT GGACTGGCCG AGGAGGTGAA AGCAATGTTC 
AAGATAGCAT GGGAGGATCC CGAGGAGCCG GACAAGGGCT TCAAGTACCT CTATCTGAGC 
ACCGAGGACT ATGCCCAGGT GGCCAATCTC AATTCGGTGC GTGCCATTCT CATCGAGGAT 
GAGGGCGAGC AGCGCTACAA AATCACCGAC ATCATCGGCA AGGACGATGG CCTGGGCGTC 
GAAAATCTGC GCTATGCCGG CCTGATTGCC GGCGAAACGT CGCAGGCGTA CGAGGAGATT 
GTCACCATTG CGATGGTCAC CTGCCGCACC ATCGGCATCG GCTCCTATGT GGTGCGTCTG 
GGCCAGCGTG TCATCCAGAT CGACAACTCG CACATTATAC TCACGGGGTA TGCGGCCCTG 
AATAAGCTGC TCGGTCGCAA GGTATACGCC TCAAACAACC AATTGGGCGG CACGCAGATC 
ATGTTCAACA ATGGCGTGAC CCACAAAACT GAGGCCATCG ACTTGGACGG CGTGTATACC 
ATCCTCGACT GGCTCTCGTA CATTCCCGCC CACATTGGCT GCGATCTGCC CATCGTTTCG 
CCCAGCGATC GTATCGATCG GCCTGTCGAT TTTATGCCCA CCAAGTCGCC GTACGATCCG 
CGCTGCATGC TGGCCGGTCG TGTGAATCCC ACCAATGCAA ATGAGTGGGA GAATGGGTTC 
TTTGATCGTG ATTCCTGGAG CGAGATTATG GCCCCGTGGG CCAAGACCGT GGTCACGGGA 
CGTGCTCGAC TGGGCGGTGT GCCTGTGGGT GTTATTGCCG TGGAGACGCG AACTGTGGAG 
GTGGAAATGC CCGCGGATCC GGCCAACCTG GATTCGGAAG CCAAGACACT GCAGCAGGCG 
GGTCAGGTGT GGTATCCCGA CTCATCCTAC AAGACGGCGC AAGCCATTAA GGACTTTGGG 
CGCGAGGAGT TGCCTCTGAT TGTGTTCGCC AACTGGCGTG GCTTCTCTGG CGGCATGAAG 
GACATGTACG AGCAGATCGT CAAGTTTGGG GCCTACATCG TTGACGGACT GCGGGAGTAC 
AAGAAGCCGG TGCTCATCTA CCTGCCGCCC AATGCCGAGC TGCGTGGCGG AGCCTGGGCC 
GTTCTGGACT CGTTGATCAA TCCGCGCTAC ATGGAAACCT ATGCGGATCC GGAGGCCCGA 
GGTGGTGTCC TCGAACCCGA GGGTATTGTG GAAATCAAAT ACAAAGAGAA GGACCTCATC 
AAGACGATAA ATCGTCTGGA TGCGACCACA ATTGCCCTAA AGAAGGAATT CGAAGAGGCA 
ACTGCAGCTG GTGATAACAC GAAGGCCGAG CTGGTGGATG AGAAGATCAA GGCTCGTCTT 
GCGCAGCTAA TGCATGTCTA TCATACGGTG GCCGTGCACT TTGCCGACCT ACACGACACG 
CCCGAGCGCA TGCTGGAGAA GGAGTGCATC AGCGAGATTG TGCCATGGCG CGGCTCGCGA 
CGTTGGCTCT ACTGGCGCCT GCGCCGCCTC CTGCTGGAGG ATGCATACAT CAAGAAGATC 
TTGCGGGCAC AGGACAATCT CTCTGTCGGA CAGGCCAAGC AGATGCTCAG ACGCTGGCTA 
GTCGAAGAAA AGGGTGCCAC AGAGGCATAT CTGTGGGACA AGAATGAGGA AATGGTGGCC 
TGGTATGAAG AGCAAACGAA TACAGAATCT ATTGTCTCGC GCAATGTGAG TTCCGTGCGA 
AGGGATGCCA TCATCTCAAC CATTTCGAAA ATGCTCGAGG ACTGTCCCGA TGTAGCGCTG 
GACGCTGTGG TTGGCCTCTG TCAAGGCCTG ACTCCCGTCA ATCGTGGAGT CGTCGTACGC 
ACACTGGCGC AAATGCAGCT GAACGAAGAG TCCTCTAGCA ATACCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015039538.1
CDS	1..7314
Translation	MLTRTLFNRCCNGRCIRMAAPHVRHNSSVIAATAPPVTATSSLSSTATGGATTKLNEKRFPKGASIKKVQFSSESLKEVDIALSDLSYHQNHNNNTTVIKNKNNNNSIVEIKKKSSVEMSEETNATPTTDAERPSFLVGDEIDEKAAEAGEAVDEFPQKMQSASRQNGDISEKRKRLRPSMSRGTGLGQDRHQDRDFHIATTEEFVKRFGGTRVINRVLIANNGIAAVKCMRSIRRWAYEMFKNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGSNNNNYANVELIVDIALRTQVQAVWAGWGHASENPKLPELLHKEGLVFLGPPERAMWALGDKVASSIVAQTAEIPTLPWSGSDLKAQYSGKKIKITSDLFARGCVTNVEQGLAAVAKIGFPVMIKASEGGGGKGIRRVDTTEEFPALFRQVQAEVPGSPIFVMKLARGARHLEVQLLADQYGNAISLFGRDCSIQRRHQKIIEEAPAIVAQPEVFEDMEKAAVRLAKMVGYVSAGTVEYLYDPEGRYFFLELNPRLQVEHPCTEMVADVNLPACQLQIGMGIPLYRLKDIRLLYGESPWGSSIIDFENPPNKPMPSGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVAASGGLHEFADSQFGHCFSWGENRQQARENLVIALKELSIRGDFRTTVEYLITLLETNRFLENTIDTAWLDALIAERVQSEKPDILLGVMCGALHIADRQITESFSSFQTSLERGQIQAANTLTNVIDVELINGGIRYKVQAAKSGANSYFLLMNSSFKEIEVHRLSDGGLLISLEGASYTTYMKEEVDRYRIVIGNQTCVFEKENDPSLLRSPSAGKLINLIVEDGAHVAKGQPFAEIEVMKMVMTLTSQEAGTVTFVRRPGAVLDPGSLLGHLELDDPSLVTKAQPCKSQFHQAENAPIPEKLNRVHNTYKSILENTLAGYCLPEPFNAQRLRDIIEKFMQSLRDPSLPLLELQEVIASISGRIPISVEKKIRKLMTLYERNITSVLAQFPSQQIASVIDSHAATLQKRADRDVFFLTTQSIVQLVQRYRNGIRGRMKAAVHELLRQYYDVESQFQHGHYDKCVGLVREHNKDDMLTVVNTIFSHSQVAKKNLLVTLLIDHLWANEPGLTDELANTLSELTSLNRAEHSRVALRSRQVLIAAHQPAYELRHNQMESIFLSAVDMYGHDFHPENLQRLILSETSIFDILHDFFYHTNRAVCNAALEVYVRRAYTSYELTCLQHLELSGGLPLVHFQFLLPTAHPNRLFSRMSSPEGLDQAAAESMGSSFVRTGAIAAFDSFEHFEMYSDEILDLLEDFVSPAMVNAKVLEAVEAADSISDGRHSTSINVSLSDPVTRANAAEEAKSTEPIHIVSVAVRETGDMDDLQMAQIFGNYCKEHNEELFQRRIRRITFAALKKRQFPKFFTYRARDKFEEDRIYRHLEPASAFHLELNRMKTYNLEALPTANQKMHLYLGRAKVSKGQEVTDYRFFIRSIIRHSDLITKEASFEYLQNEGERVLLEAMDELEVAFSHPHAKRTDCNHIFLNFVPTVIMDPAKIEESVTKMIMRYGPRLWKLRVLQAELKMVIRQSPQSPTQAVRLCIANDSGYFLDISMYTEQTEPETGIIKFKAYGEKQGSLHGHPISTPYMTKDFLQQKRFQAQSSGTTYVYDVPDMFRQMTERHWKEFSNARPSVDIRIPDKILIECKELVLEGDSLIEMQRLPGENNCGMVAWRIVLATPEYPNGREIIVIANDLTYLIGSFGIKEDVLFAKASQLARERKVPRIYISVNSGARIGLAEEVKAMFKIAWEDPEEPDKGFKYLYLSTEDYAQVANLNSVRAILIEDEGEQRYKITDIIGKDDGLGVENLRYAGLIAGETSQAYEEIVTIAMVTCRTIGIGSYVVRLGQRVIQIDNSHIILTGYAALNKLLGRKVYASNNQLGGTQIMFNNGVTHKTEAIDLDGVYTILDWLSYIPAHIGCDLPIVSPSDRIDRPVDFMPTKSPYDPRCMLAGRVNPTNANEWENGFFDRDSWSEIMAPWAKTVVTGRARLGGVPVGVIAVETRTVEVEMPADPANLDSEAKTLQQAGQVWYPDSSYKTAQAIKDFGREELPLIVFANWRGFSGGMKDMYEQIVKFGAYIVDGLREYKKPVLIYLPPNAELRGGAWAVLDSLINPRYMETYADPEARGGVLEPEGIVEIKYKEKDLIKTINRLDATTIALKKEFEEATAAGDNTKAELVDEKIKARLAQLMHVYHTVAVHFADLHDTPERMLEKECISEIVPWRGSRRWLYWRLRRLLLEDAYIKKILRAQDNLSVGQAKQMLRRWLVEEKGATEAYLWDKNEEMVAWYEEQTNTESIVSRNVSSVRRDAIISTISKMLEDCPDVALDAVVGLCQGLTPVNRGVVVRTLAQMQLNEESSSNTQG

Target ORF information:

RefSeq Version	XM_015184052.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant D (Dpse\GA10832), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184052.1

ORF Insert Sequence:

ATGTTAACCA GGACGCTGTT TAACCGCTGC TGCAACGGAC GCTGCATCAG AATGGCGGCT 
CCACACGTGA GGCACAACTC CAGCGTGATT GCAGCTACAG CACCACCAGT CACAGCCACA 
AGCAGCTTGT CGTCGACCGC AACGGGAGGA GCCACAACAA AGCTGAACGA GAAGCGATTC 
CCCAAAGGAG CCAGCATCAA GAAGGTTCAG TTCTCCAGCG AAAGCCTTAA GGAGGTGGAT 
ATTGCCTTGT CCGATCTGAG CTATCACCAG AACCACAACA ACAACACAAC TGTCATCAAG 
AACAAGAACA ACAATAATAG CATTGTCGAA ATCAAAAAGA AATCATCAGT CGAAATGAGC 
GAGGAAACAA ATGCAACGCC AACAACGGAT GCGGAGCGTC CAAGTTTCTT GGTGGGCGAT 
GAGATTGACG AAAAGGCAGC CGAAGCCGGC GAGGCTGTAG ACGAGTTCCC CCAGAAGATG 
CAGAGCGCGT CCCGGCAGAA TGGCGATATA TCGGAGAAAC GCAAGCGATT AAGACCGAGC 
ATGTCGCGTG GCACTGGCCT GGGCCAGGAC CGCCATCAGG ATCGTGACTT TCATATAGCC 
ACCACCGAGG AGTTTGTGAA GCGTTTCGGC GGTACACGTG TCATTAACCG CGTCCTCATT 
GCCAACAATG GCATTGCGGC TGTCAAGTGC ATGCGTTCCA TTCGCCGATG GGCCTACGAG 
ATGTTCAAGA ACGAGCGGGC CATCCGATTT GTGGTGATGG TCACCCCGGA GGACCTGAAG 
GCGAATGCCG AGTACATCAA GATGGCCGAT CACTATGTCC CCGTGCCCGG CGGTTCCAAC 
AACAACAACT ATGCGAATGT TGAGCTCATT GTGGACATTG CACTTCGCAC ACAAGTGCAG 
GCTGTCTGGG CTGGATGGGG TCATGCCTCG GAGAACCCCA AGTTGCCGGA GCTGCTGCAC 
AAGGAGGGTT TGGTGTTCCT CGGGCCACCG GAGCGCGCCA TGTGGGCGCT GGGCGACAAG 
GTGGCCTCCT CGATTGTGGC CCAAACGGCC GAAATACCCA CACTACCATG GTCCGGCTCC 
GACCTGAAAG CCCAGTACAG CGGCAAGAAG ATCAAGATCA CCAGCGACCT TTTTGCCCGC 
GGCTGTGTGA CTAATGTGGA GCAGGGTCTG GCGGCTGTCG CCAAGATTGG CTTTCCCGTG 
ATGATCAAGG CTTCGGAAGG CGGCGGCGGC AAGGGCATTC GTCGCGTGGA CACCACCGAA 
GAGTTCCCCG CCCTGTTCCG TCAGGTGCAG GCCGAGGTGC CCGGCTCCCC CATCTTTGTG 
ATGAAGCTGG CTCGCGGCGC ACGCCACTTG GAAGTGCAGC TGCTGGCGGA TCAGTACGGA 
AATGCCATCA GTCTCTTTGG CCGCGATTGC TCCATACAGC GTCGCCATCA AAAGATCATC 
GAGGAGGCCC CGGCCATTGT GGCCCAGCCG GAGGTGTTTG AGGACATGGA AAAGGCGGCC 
GTGCGTCTGG CCAAGATGGT GGGCTATGTG AGCGCCGGCA CCGTGGAGTA CCTATACGAT 
CCGGAGGGCA GGTACTTCTT CCTGGAGCTG AACCCCCGTC TGCAGGTGGA GCATCCCTGC 
ACGGAGATGG TGGCCGATGT CAATCTACCG GCCTGCCAGC TGCAGATCGG CATGGGCATC 
CCCCTATACC GCCTCAAGGA CATTCGCCTG TTGTACGGTG AGTCGCCGTG GGGCTCCTCC 
ATCATTGACT TTGAGAATCC GCCGAACAAG CCCATGCCCT CGGGCCATGT GATCGCTGCG 
CGCATCACCT CCGAGAATCC CGACGAGGGC TTCAAGCCCA GCTCGGGCAC AGTTCAGGAG 
CTCAACTTCC GCTCCAGCAA GAACGTCTGG GGCTACTTCA GTGTGGCCGC CTCCGGGGGA 
TTGCACGAGT TTGCCGACTC GCAGTTCGGT CACTGCTTCT CCTGGGGCGA GAACCGGCAG 
CAGGCGCGCG AGAATCTCGT GATTGCCCTC AAAGAGCTGT CGATCCGTGG CGATTTCCGC 
ACCACCGTCG AGTACCTGAT CACGCTGCTC GAGACGAATC GCTTCCTGGA GAACACCATC 
GACACAGCCT GGCTGGATGC TCTGATTGCC GAGCGCGTGC AGTCCGAGAA GCCGGACATA 
CTGCTGGGCG TGATGTGCGG GGCCCTGCAC ATTGCCGATC GCCAGATCAC CGAATCCTTC 
TCCAGCTTCC AAACGTCGCT GGAGAGGGGC CAGATCCAGG CGGCCAACAC CCTGACGAAT 
GTGATAGACG TGGAGCTGAT CAACGGAGGG ATCCGGTACA AGGTTCAAGC GGCCAAGAGC 
GGCGCCAACT CGTACTTCCT GCTGATGAAC AGCTCCTTCA AGGAGATCGA GGTGCACCGC 
CTGTCCGACG GCGGCCTGCT GATCTCCCTC GAGGGCGCCT CGTACACCAC CTACATGAAG 
GAGGAGGTGG ACCGCTACCG CATCGTTATT GGCAACCAGA CGTGCGTTTT CGAGAAGGAG 
AACGATCCGT CGCTGCTGCG CAGCCCATCG GCGGGAAAGC TCATCAATCT GATTGTGGAG 
GATGGGGCAC ATGTGGCCAA GGGCCAGCCC TTTGCCGAGA TCGAGGTGAT GAAGATGGTG 
ATGACCCTCA CCTCGCAGGA GGCAGGCACG GTGACCTTTG TGCGGCGTCC AGGCGCCGTC 
CTCGACCCGG GCTCCCTGCT GGGCCACCTG GAGCTCGATG ATCCCTCGCT GGTGACCAAG 
GCGCAGCCCT GCAAGAGCCA GTTCCACCAG GCCGAGAACG CGCCCATTCC CGAGAAGCTG 
AACCGCGTGC ACAACACGTA CAAGAGCATT TTGGAGAACA CCTTGGCCGG CTACTGCCTG 
CCGGAGCCCT TCAATGCCCA GCGGCTGCGC GACATCATCG AGAAGTTCAT GCAGAGCCTG 
AGGGATCCAT CGCTGCCGCT GCTGGAACTC CAGGAGGTGA TTGCCTCCAT CTCGGGCCGC 
ATCCCCATCT CCGTAGAGAA GAAGATTCGC AAGCTGATGA CCCTCTACGA GCGGAACATC 
ACCAGTGTGT TGGCCCAGTT TCCCTCCCAG CAGATCGCCA GCGTCATCGA CAGCCACGCG 
GCCACCTTGC AGAAGCGCGC CGATCGCGAC GTCTTCTTCC TGACCACCCA GAGCATTGTG 
CAGCTGGTGC AGCGCTACCG GAATGGCATT CGCGGCCGCA TGAAGGCCGC CGTTCACGAA 
CTGCTGCGCC AGTACTACGA CGTGGAGTCG CAGTTCCAGC ACGGCCACTA CGACAAGTGC 
GTGGGCCTGG TGCGGGAGCA CAACAAGGAT GACATGCTGA CGGTGGTGAA CACGATATTC 
TCGCACTCGC AAGTGGCCAA GAAGAACTTG CTCGTTACGC TGCTAATCGA CCATCTGTGG 
GCCAACGAGC CGGGACTCAC CGACGAGCTG GCCAACACCC TCAGCGAGCT AACGTCCCTC 
AACAGGGCCG AGCACTCGCG GGTGGCTCTG CGCTCTCGCC AGGTACTGAT TGCCGCCCAC 
CAGCCGGCAT ACGAGCTGCG CCACAACCAA ATGGAGTCCA TATTCCTGTC GGCCGTAGAC 
ATGTACGGCC ACGACTTCCA TCCGGAGAAT CTGCAGCGCC TGATCCTCTC GGAGACCTCA 
ATCTTTGACA TTCTCCACGA TTTCTTCTAC CACACGAACC GAGCCGTGTG CAACGCCGCC 
CTCGAGGTGT ACGTGAGGCG CGCCTACACC TCCTATGAGC TGACTTGCCT GCAGCACTTG 
GAGCTGTCCG GCGGCCTGCC GCTGGTGCAT TTCCAGTTCC TGCTGCCCAC GGCCCATCCG 
AATCGTCTGT TCTCGCGCAT GTCCTCGCCG GAGGGACTAG ACCAGGCAGC CGCCGAGTCG 
ATGGGCAGCT CCTTTGTCCG CACCGGAGCG ATTGCCGCCT TCGATTCGTT CGAGCACTTT 
GAGATGTACT CGGACGAGAT ACTGGACCTT CTCGAGGACT TTGTCTCGCC CGCGATGGTC 
AATGCCAAGG TGCTGGAGGC TGTGGAGGCA GCCGACTCCA TCTCGGACGG CCGTCACAGC 
ACCTCCATCA ACGTCTCGCT GTCGGATCCC GTCACCAGAG CCAATGCCGC CGAGGAGGCC 
AAGTCCACGG AGCCCATTCA CATTGTGAGT GTGGCTGTGC GGGAGACCGG AGATATGGAC 
GACTTGCAGA TGGCCCAGAT TTTCGGGAAC TACTGCAAGG AGCACAACGA GGAGCTCTTC 
CAGCGCCGCA TTCGGAGGAT AACGTTTGCC GCTTTGAAGA AGCGCCAGTT CCCCAAGTTC 
TTTACATACC GCGCACGCGA CAAGTTCGAG GAGGATCGCA TTTATCGGCA TCTGGAGCCG 
GCCTCTGCCT TCCATTTGGA GCTAAACCGC ATGAAGACCT ACAACCTGGA GGCCCTGCCG 
ACGGCCAACC AGAAGATGCA TCTGTATTTG GGCAGGGCAA AGGTGTCCAA GGGACAGGAG 
GTGACCGACT ATCGTTTCTT CATCCGCTCG ATCATCCGCC ACTCGGACCT CATCACCAAG 
GAGGCCTCAT TCGAGTACCT CCAGAACGAG GGCGAACGGG TGCTGCTGGA GGCCATGGAC 
GAGCTGGAGG TGGCCTTCTC CCACCCGCAT GCCAAGCGCA CCGATTGCAA TCACATCTTT 
CTCAATTTCG TGCCCACCGT TATTATGGAC CCGGCCAAGA TCGAGGAGTC CGTCACCAAG 
ATGATCATGC GCTATGGCCC CCGCCTGTGG AAGCTGCGCG TCCTCCAGGC CGAGCTGAAG 
ATGGTCATCC GCCAGTCGCC GCAGTCGCCC ACCCAAGCGG TGCGCCTCTG TATTGCCAAC 
GATTCTGGCT ACTTCCTGGA CATCTCCATG TACACGGAGC AGACGGAGCC GGAGACGGGA 
ATTATCAAGT TCAAGGCCTA TGGAGAGAAG CAGGGTTCCC TTCACGGCCA TCCCATTTCG 
ACGCCCTACA TGACCAAGGA CTTCCTGCAG CAGAAGCGCT TCCAGGCGCA GTCGAGCGGC 
ACCACGTACG TGTACGATGT GCCTGACATG TTCCGCCAGA TGACCGAGCG CCACTGGAAG 
GAGTTCTCCA ATGCCCGGCC CTCCGTGGAC ATACGCATAC CCGACAAGAT TCTGATTGAA 
TGCAAGGAGC TGGTGCTGGA GGGCGACAGC CTCATTGAGA TGCAGCGTCT GCCGGGCGAA 
AACAATTGCG GCATGGTGGC CTGGCGCATT GTCCTGGCCA CGCCGGAGTA CCCGAATGGG 
CGCGAGATCA TTGTGATAGC TAATGATCTG ACCTACCTGA TTGGCTCCTT TGGCATCAAA 
GAGGATGTGC TGTTCGCCAA GGCTTCCCAG TTGGCGCGCG AACGGAAAGT GCCCAGGATC 
TATATATCGG TGAACAGCGG TGCCCGCATT GGACTGGCCG AGGAGGTGAA AGCAATGTTC 
AAGATAGCAT GGGAGGATCC CGAGGAGCCG GACAAGGGCT TCAAGTACCT CTATCTGAGC 
ACCGAGGACT ATGCCCAGGT GGCCAATCTC AATTCGGTGC GTGCCATTCT CATCGAGGAT 
GAGGGCGAGC AGCGCTACAA AATCACCGAC ATCATCGGCA AGGACGATGG CCTGGGCGTC 
GAAAATCTGC GCTATGCCGG CCTGATTGCC GGCGAAACGT CGCAGGCGTA CGAGGAGATT 
GTCACCATTG CGATGGTCAC CTGCCGCACC ATCGGCATCG GCTCCTATGT GGTGCGTCTG 
GGCCAGCGTG TCATCCAGAT CGACAACTCG CACATTATAC TCACGGGGTA TGCGGCCCTG 
AATAAGCTGC TCGGTCGCAA GGTATACGCC TCAAACAACC AATTGGGCGG CACGCAGATC 
ATGTTCAACA ATGGCGTGAC CCACAAAACT GAGGCCATCG ACTTGGACGG CGTGTATACC 
ATCCTCGACT GGCTCTCGTA CATTCCCGCC CACATTGGCT GCGATCTGCC CATCGTTTCG 
CCCAGCGATC GTATCGATCG GCCTGTCGAT TTTATGCCCA CCAAGTCGCC GTACGATCCG 
CGCTGCATGC TGGCCGGTCG TGTGAATCCC ACCAATGCAA ATGAGTGGGA GAATGGGTTC 
TTTGATCGTG ATTCCTGGAG CGAGATTATG GCCCCGTGGG CCAAGACCGT GGTCACGGGA 
CGTGCTCGAC TGGGCGGTGT GCCTGTGGGT GTTATTGCCG TGGAGACGCG AACTGTGGAG 
GTGGAAATGC CCGCGGATCC GGCCAACCTG GATTCGGAAG CCAAGACACT GCAGCAGGCG 
GGTCAGGTGT GGTATCCCGA CTCATCCTAC AAGACGGCGC AAGCCATTAA GGACTTTGGG 
CGCGAGGAGT TGCCTCTGAT TGTGTTCGCC AACTGGCGTG GCTTCTCTGG CGGCATGAAG 
GACATGTACG AGCAGATCGT CAAGTTTGGG GCCTACATCG TTGACGGACT GCGGGAGTAC 
AAGAAGCCGG TGCTCATCTA CCTGCCGCCC AATGCCGAGC TGCGTGGCGG AGCCTGGGCC 
GTTCTGGACT CGTTGATCAA TCCGCGCTAC ATGGAAACCT ATGCGGATCC GGAGGCCCGA 
GGTGGTGTCC TCGAACCCGA GGGTATTGTG GAAATCAAAT ACAAAGAGAA GGACCTCATC 
AAGACGATAA ATCGTCTGGA TGCGACCACA ATTGCCCTAA AGAAGGAATT CGAAGAGGCA 
ACTGCAGCTG GTGATAACAC GAAGGCCGAG CTGGTGGATG AGAAGATCAA GGCTCGTCTT 
GCGCAGCTAA TGCATGTCTA TCATACGGTG GCCGTGCACT TTGCCGACCT ACACGACACG 
CCCGAGCGCA TGCTGGAGAA GGAGTGCATC AGCGAGATTG TGCCATGGCG CGGCTCGCGA 
CGTTGGCTCT ACTGGCGCCT GCGCCGCCTC CTGCTGGAGG ATGCATACAT CAAGAAGATC 
TTGCGGGCAC AGGACAATCT CTCTGTCGGA CAGGCCAAGC AGATGCTCAG ACGCTGGCTA 
GTCGAAGAAA AGGGTGCCAC AGAGGCATAT CTGTGGGACA AGAATGAGGA AATGGTGGCC 
TGGTATGAAG AGCAAACGAA TACAGAATCT ATTGTCTCGC GCAATGTGAG TTCCGTGCGA 
AGGGATGCCA TCATCTCAAC CATTTCGAAA ATGCTCGAGG ACTGTCCCGA TGTAGCGCTG 
GACGCTGTGG TTGGCCTCTG TCAAGGCCTG ACTCCCGTCA ATCGTGGAGT CGTCGTACGC 
ACACTGGCGC AAATGCAGCT GAACGAAGAG TCCTCTAGCA ATACCCAGGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA10832 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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