Dpse\GA19959 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA19959
Entrez Gene ID	4802838
Full Name	GA19959 gene product from transcript GA19959-RA
Synonyms	GA19959,dpse_GLEANR_6241
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001359637.3	XP_001359674.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA19959 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA19959 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe19852	XM_001359637.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19959), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA19959 ( XM_001359637.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001359674.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19959), mRNA.

Price & Availability↑

CloneID	ODe19852
Clone ID Related Accession (Same CDS sequence)	XM_001359637.3
Accession Version	XM_001359637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001359637.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAATG AGCAGGAGGA GTTTGGACAC AGACATGCAC ACATGCAGCA TACACACCAT 
CCACGAGTTT CCATGTCGTA CGGAAATGTG GGTCAGAATC CGGACCAGGA GACCCATGGC 
GGCCCTGCGT ATACTCTATA CTCCATATAT GAACAGCCGC GTGATAGTTA CATGCTAATG 
GGCCCATACA TGTCGACGCC CGCCGAGTCG ATTAGTAGCC GTGCTACCAC GTTCCAGTCC 
CACACTCCGC CTTCCTATAC GGCCCTGGTG GCATCACATC GCGAAATAGC CAGCACAAGC 
TATAGGACGA GTTCTGGTCG ATGCGACAAA TGCAGTTCTG TCACAACTTT GTGCCGCTGT 
CAGGTGTCAC CTGCGGCCAG CACCAGTCGG TACGCAGACC AGGTGCATTC CACTCAGAGC 
CTGCCTCAAG AGTCACCGCC TCAGTTCTTT GAGCTGCCCT CATCGTCGAC ATCGACTGGG 
TCGACTCTGC CAACGACGAC GGTTAACTCC AGCCCCGGAC TCATTCCAAA TCCCAAACAA 
CAGAAGACTG AAACACTGAG GGAGCATTTG TCGTTGCAAC AGGACAATGA CAATGAGAAT 
GAAAATGCAA ATGCACATGC CCATGCAGTG GTGGCCTCTG CCTCTGGCAA CGGCACTTGG 
CCAGCTGCAG CCATTGAAGA CCACGCAATG GATGCCTCCG CAGTGGTTTA CGAGATGCCC 
TCCAGTTCGC TGCTGAGCAT CAGCCCCTTT GAGGCCAGTA GTAGCAATAT TCCCCAGAGC 
TCTGGTCTTT CCAGTGCCTA CGATGATGTG ACATCCAGTG CTTCTGATAC AGAGCACGTT 
CAGTTTGCAA ACACTCGCAC TACCAATACG ACTACAACCA CGTCGGCGTT ACAGGCTCAC 
AAGAAGAAGG CAATGCAGGC AGCAAATCGC TCCGGAAACG GAAGCGGCAA CGGCAACGGG 
GATGCTTCTT CCTCCTCTTC GGCCACTGAA CTAGACGCCA CACCGAGCAC TAGTGCCCAA 
GCTCGGCGAC AGCAGACAAA TTTATTTAAT AGCTACCAGC AGCTCGCGAA CGTATTGGAG 
CCTGAGAACG ATAGTAGTGA TGATGGCTGG GATCTTCGCA TGCGTAATCG TCCTCCCCAA 
TCGAAACCGG CTGAACCTGT TGTACGGGGC AACAACACCA ACAAAAACCT GACTGATCGG 
CATACAGATC ATAACTACTA CAATAGCCGC AGAGATGTGT CTACGAGGGC GCCTCCAGAT 
AGCACTTTTG TGTCCGGCGC TTCTTGTTCT ACGGCGACAC ACACAACCTG CAGTGTGGAT 
TATGGGACTA GAGATCTGTG CGGGGCTCTT CGACCGCCTC AGAGATCGTC TGATGCGTCT 
GCTGCTGATG CTCTGTCTCC GTCCACTACA TCGGATCAGG AGCAAGAGCA TCAGCAGCAG 
GAGCAGGGGC ATGGGCAACA ACAGGAGCAG CAGCAGCAGG TGCAGCAGCA GAGACGGAGA 
CAAGAAACCA CAGGCCAACG AAGCTCCCAT GGCGCCAATG TAAACAGTGG TATTATTCGA 
AATCGTGTGC GTTCCATTGA CGAAATTGCG CATCCACCCA GAAAACAACC GCGCTTAGAG 
GTTGAAGGAG CATCCTCTTC CCACTCCACC ACAAAACTGT CGCCGACGAA CTTTAATTCC 
TTTTTGCCGG GCAGGACAAG GCATCCCACC TATGCCGAGC CCGCGAGTAG CTCCTTACAG 
CAGACCTCAC AGCAGACGCA GCAGCCCTTG AACGGATCTT GCGTGATTAC CTATGTGGGT 
AGACCAAGAG AGAGGCAGTC CCCTGGGGAG ACCCAGAATC AGGCTTCCAC TTCCACGGGC 
AGGCGTAACA ATTTGAACCG CGGTGATGTT GTGCTCACTG CTCCAGATCT GCAACTGGAC 
GATTGGTCAT CCGACTCCAA TGATGACGAT GTGGTGTTTG TGCACTCGAC ACGAGAGCCA 
ATACTCTCGA TTGATTTGAC ATCTGATGAT GATGCCGCCG TTAATGAAAC ACAACAGCAG 
CGAGCCCGGG AATGGGCACG GGAACATGAG CATATGTGGG AACAGCCACT TGCAGCCCTG 
GAACGACGTC CCATTTTCAA CTATGCCTAC TCCATTCCAT ACCCTCGAAG GGACCGCCGG 
ACAGAGCCGA GCAATGCTGG CACCTCATTT GGCTTCTATA GCGGAGGGCT GCCGGATCGA 
ACGGCCATTA CCGATCACAA CTACAGCTAC GATCATAATC ATACGCAAGC ACCCGCCCCG 
CTGATGGAAG AGCCCGGTCC GGCAGCGAGT AACTATTTTC CTCGTCAAAC TGCAGGTTGC 
ACTCCTCGGT GTCCCACCCT GGACTCGCAG CGGTACTTTC AACCGATCAC TCCTCCATCC 
ATGTGGCTCT TTGGAGCGGC GCCAGAGACC ACGCCCACAG AGGGCTACAG TCGTATGCCC 
AGAGCCGCCC CCACTACGAC TTCTGGCTCC GCCGAGCAAT CGAGCCAGCA GTCGCAACGT 
AGACGCACTA GTCCAGCTAA CGTCTACCAT CGTTATCAGC CGTATTACGT GCCTCACTAT 
GCGATCACTC CACTGCCCAC AGTAATGGAA GCAGTGTATT CGCCCACCCA TCCGCATGGT 
CCGCAGCCTC CAAATCCGCA TCCACATCCA CATCCGCATC CGCACTATCA GGGCGGACAA 
GGCGGTAGCA GCCGCAGTTC GTTAAGTGGC GCTATGAGTG CTGCCGCTGT AGCGGCAACA 
GCGGCTGCGG CCCAAGCTCA ATCCTTCAGC GAGCTCCTGT CACTGGCCAG TGTTCACAAT 
GAGGCAGAAA CCAACATGGA TAATCGGTTG GATAGAGGCC AATCACCTCT CCCCATCGTG 
CCCCGAGCCC ATCCCATGGC CCATGCTATA AATGCCGCCT CATCTGTCGG CTTGGTGAGC 
CAGCCACCGC CTGTGGCAAC GACTAATGGT AATCTGGCAG CTGCTGGTGT CTCGCCTCCT 
CCGCCGCTGG AAATGTATTC GGGTCGATCC ACCATTCCCT ACTGTGGCTC CCCGCCTTTC 
AGCGTGCGAC GGTCGGAGGC AGCGTACAGC AATCGGCAGC ATTATCAGCC GCAGCCGCAT 
CAAGGTCATC AGGCCCCACA AAATCAAATG CACGCGCATA TACATCCCCC TCATCGAGCA 
AGTGCCATTG TGGCCACCTC GCTGACACCG GCGCCGCCAT ATCCGGTACA TCAGAATCTG 
TGGTATCGCC AGCAGAGCAT GCAGGAAATG CACCGTCGCC ACATGACGCC CACGCCCATT 
GATCTCTCCT CGAATGCTCC CATTTTGACC AGCAGTCTGC GGACCCGCTA CCTGCATAGC 
ATTTGCAATT GCGTGCATGC CAGGAACGGC CCGGTCTCTA GCTTGGATCC GGCCTACTAT 
CCGCCCTACG ATGGCAATGC CAGTGCTGCG GCTCCGTCCC AGCAGATACC TTCCAGTGGT 
GGCCAGGCCA CGACCGTATC CGTTGCCCAG CCACAGCACT CTCAGTCAAA CTTGCGACAT 
ATGCAAGTGC AGCACCAGCA GCCTCAGGCC CAGCAGCAGT CCCCGCCGGT TCTGCAGCAT 
CTGCAGCGTC AGCGTGCGAT CCATCATCAT ATGTTCCACC ATCATTACTC GCCGCTGCAT 
TTGGAAATTG GACTTGCAAC GCCGCTGTCG CTCGGCTCAC GGATAGTGAT TGGAGCAGCA 
CGTCCAAATC GGGGGGCCAC TTTGGAAACA ATTGAGCGCA ATACCTTGCC CCACAAGTAC 
CGGCGTTTGC GTCGACCCAG CGAATCAGAC GAGGACGCGG AGAAGTGTGC TATATGCTTG 
TCTCTGTTCG AGATCGAGAA CGATGTGCGC CGCCTTCCCT GTATGCATCT ATTTCATACC 
GATTGCGTTG ATCAATGGTT AGTCACCAAT AAACACTGCC CTATTTGCCG AGTCGACATC 
GAGACCCACA TGGCGAATGA CGCACTCAAC TCCAGTGGGG TCACTGATGC CGCCAATGCC 
GCCGCCTTAT GACATTGTGT GCATGGAATC TTCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001359674.3
CDS	163..4221
Translation	MENEQEEFGHRHAHMQHTHHPRVSMSYGNVGQNPDQETHGGPAYTLYSIYEQPRDSYMLMGPYMSTPAESISSRATTFQSHTPPSYTALVASHREIASTSYRTSSGRCDKCSSVTTLCRCQVSPAASTSRYADQVHSTQSLPQESPPQFFELPSSSTSTGSTLPTTTVNSSPGLIPNPKQQKTETLREHLSLQQDNDNENENANAHAHAVVASASGNGTWPAAAIEDHAMDASAVVYEMPSSSLLSISPFEASSSNIPQSSGLSSAYDDVTSSASDTEHVQFANTRTTNTTTTTSALQAHKKKAMQAANRSGNGSGNGNGDASSSSSATELDATPSTSAQARRQQTNLFNSYQQLANVLEPENDSSDDGWDLRMRNRPPQSKPAEPVVRGNNTNKNLTDRHTDHNYYNSRRDVSTRAPPDSTFVSGASCSTATHTTCSVDYGTRDLCGALRPPQRSSDASAADALSPSTTSDQEQEHQQQEQGHGQQQEQQQQVQQQRRRQETTGQRSSHGANVNSGIIRNRVRSIDEIAHPPRKQPRLEVEGASSSHSTTKLSPTNFNSFLPGRTRHPTYAEPASSSLQQTSQQTQQPLNGSCVITYVGRPRERQSPGETQNQASTSTGRRNNLNRGDVVLTAPDLQLDDWSSDSNDDDVVFVHSTREPILSIDLTSDDDAAVNETQQQRAREWAREHEHMWEQPLAALERRPIFNYAYSIPYPRRDRRTEPSNAGTSFGFYSGGLPDRTAITDHNYSYDHNHTQAPAPLMEEPGPAASNYFPRQTAGCTPRCPTLDSQRYFQPITPPSMWLFGAAPETTPTEGYSRMPRAAPTTTSGSAEQSSQQSQRRRTSPANVYHRYQPYYVPHYAITPLPTVMEAVYSPTHPHGPQPPNPHPHPHPHPHYQGGQGGSSRSSLSGAMSAAAVAATAAAAQAQSFSELLSLASVHNEAETNMDNRLDRGQSPLPIVPRAHPMAHAINAASSVGLVSQPPPVATTNGNLAAAGVSPPPPLEMYSGRSTIPYCGSPPFSVRRSEAAYSNRQHYQPQPHQGHQAPQNQMHAHIHPPHRASAIVATSLTPAPPYPVHQNLWYRQQSMQEMHRRHMTPTPIDLSSNAPILTSSLRTRYLHSICNCVHARNGPVSSLDPAYYPPYDGNASAAAPSQQIPSSGGQATTVSVAQPQHSQSNLRHMQVQHQQPQAQQQSPPVLQHLQRQRAIHHHMFHHHYSPLHLEIGLATPLSLGSRIVIGAARPNRGATLETIERNTLPHKYRRLRRPSESDEDAEKCAICLSLFEIENDVRRLPCMHLFHTDCVDQWLVTNKHCPICRVDIETHMANDALNSSGVTDAANAAAL*HCVHGIFM

Target ORF information:

RefSeq Version	XM_001359637.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19959), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001359637.3

ORF Insert Sequence:

ATGGAAAATG AGCAGGAGGA GTTTGGACAC AGACATGCAC ACATGCAGCA TACACACCAT 
CCACGAGTTT CCATGTCGTA CGGAAATGTG GGTCAGAATC CGGACCAGGA GACCCATGGC 
GGCCCTGCGT ATACTCTATA CTCCATATAT GAACAGCCGC GTGATAGTTA CATGCTAATG 
GGCCCATACA TGTCGACGCC CGCCGAGTCG ATTAGTAGCC GTGCTACCAC GTTCCAGTCC 
CACACTCCGC CTTCCTATAC GGCCCTGGTG GCATCACATC GCGAAATAGC CAGCACAAGC 
TATAGGACGA GTTCTGGTCG ATGCGACAAA TGCAGTTCTG TCACAACTTT GTGCCGCTGT 
CAGGTGTCAC CTGCGGCCAG CACCAGTCGG TACGCAGACC AGGTGCATTC CACTCAGAGC 
CTGCCTCAAG AGTCACCGCC TCAGTTCTTT GAGCTGCCCT CATCGTCGAC ATCGACTGGG 
TCGACTCTGC CAACGACGAC GGTTAACTCC AGCCCCGGAC TCATTCCAAA TCCCAAACAA 
CAGAAGACTG AAACACTGAG GGAGCATTTG TCGTTGCAAC AGGACAATGA CAATGAGAAT 
GAAAATGCAA ATGCACATGC CCATGCAGTG GTGGCCTCTG CCTCTGGCAA CGGCACTTGG 
CCAGCTGCAG CCATTGAAGA CCACGCAATG GATGCCTCCG CAGTGGTTTA CGAGATGCCC 
TCCAGTTCGC TGCTGAGCAT CAGCCCCTTT GAGGCCAGTA GTAGCAATAT TCCCCAGAGC 
TCTGGTCTTT CCAGTGCCTA CGATGATGTG ACATCCAGTG CTTCTGATAC AGAGCACGTT 
CAGTTTGCAA ACACTCGCAC TACCAATACG ACTACAACCA CGTCGGCGTT ACAGGCTCAC 
AAGAAGAAGG CAATGCAGGC AGCAAATCGC TCCGGAAACG GAAGCGGCAA CGGCAACGGG 
GATGCTTCTT CCTCCTCTTC GGCCACTGAA CTAGACGCCA CACCGAGCAC TAGTGCCCAA 
GCTCGGCGAC AGCAGACAAA TTTATTTAAT AGCTACCAGC AGCTCGCGAA CGTATTGGAG 
CCTGAGAACG ATAGTAGTGA TGATGGCTGG GATCTTCGCA TGCGTAATCG TCCTCCCCAA 
TCGAAACCGG CTGAACCTGT TGTACGGGGC AACAACACCA ACAAAAACCT GACTGATCGG 
CATACAGATC ATAACTACTA CAATAGCCGC AGAGATGTGT CTACGAGGGC GCCTCCAGAT 
AGCACTTTTG TGTCCGGCGC TTCTTGTTCT ACGGCGACAC ACACAACCTG CAGTGTGGAT 
TATGGGACTA GAGATCTGTG CGGGGCTCTT CGACCGCCTC AGAGATCGTC TGATGCGTCT 
GCTGCTGATG CTCTGTCTCC GTCCACTACA TCGGATCAGG AGCAAGAGCA TCAGCAGCAG 
GAGCAGGGGC ATGGGCAACA ACAGGAGCAG CAGCAGCAGG TGCAGCAGCA GAGACGGAGA 
CAAGAAACCA CAGGCCAACG AAGCTCCCAT GGCGCCAATG TAAACAGTGG TATTATTCGA 
AATCGTGTGC GTTCCATTGA CGAAATTGCG CATCCACCCA GAAAACAACC GCGCTTAGAG 
GTTGAAGGAG CATCCTCTTC CCACTCCACC ACAAAACTGT CGCCGACGAA CTTTAATTCC 
TTTTTGCCGG GCAGGACAAG GCATCCCACC TATGCCGAGC CCGCGAGTAG CTCCTTACAG 
CAGACCTCAC AGCAGACGCA GCAGCCCTTG AACGGATCTT GCGTGATTAC CTATGTGGGT 
AGACCAAGAG AGAGGCAGTC CCCTGGGGAG ACCCAGAATC AGGCTTCCAC TTCCACGGGC 
AGGCGTAACA ATTTGAACCG CGGTGATGTT GTGCTCACTG CTCCAGATCT GCAACTGGAC 
GATTGGTCAT CCGACTCCAA TGATGACGAT GTGGTGTTTG TGCACTCGAC ACGAGAGCCA 
ATACTCTCGA TTGATTTGAC ATCTGATGAT GATGCCGCCG TTAATGAAAC ACAACAGCAG 
CGAGCCCGGG AATGGGCACG GGAACATGAG CATATGTGGG AACAGCCACT TGCAGCCCTG 
GAACGACGTC CCATTTTCAA CTATGCCTAC TCCATTCCAT ACCCTCGAAG GGACCGCCGG 
ACAGAGCCGA GCAATGCTGG CACCTCATTT GGCTTCTATA GCGGAGGGCT GCCGGATCGA 
ACGGCCATTA CCGATCACAA CTACAGCTAC GATCATAATC ATACGCAAGC ACCCGCCCCG 
CTGATGGAAG AGCCCGGTCC GGCAGCGAGT AACTATTTTC CTCGTCAAAC TGCAGGTTGC 
ACTCCTCGGT GTCCCACCCT GGACTCGCAG CGGTACTTTC AACCGATCAC TCCTCCATCC 
ATGTGGCTCT TTGGAGCGGC GCCAGAGACC ACGCCCACAG AGGGCTACAG TCGTATGCCC 
AGAGCCGCCC CCACTACGAC TTCTGGCTCC GCCGAGCAAT CGAGCCAGCA GTCGCAACGT 
AGACGCACTA GTCCAGCTAA CGTCTACCAT CGTTATCAGC CGTATTACGT GCCTCACTAT 
GCGATCACTC CACTGCCCAC AGTAATGGAA GCAGTGTATT CGCCCACCCA TCCGCATGGT 
CCGCAGCCTC CAAATCCGCA TCCACATCCA CATCCGCATC CGCACTATCA GGGCGGACAA 
GGCGGTAGCA GCCGCAGTTC GTTAAGTGGC GCTATGAGTG CTGCCGCTGT AGCGGCAACA 
GCGGCTGCGG CCCAAGCTCA ATCCTTCAGC GAGCTCCTGT CACTGGCCAG TGTTCACAAT 
GAGGCAGAAA CCAACATGGA TAATCGGTTG GATAGAGGCC AATCACCTCT CCCCATCGTG 
CCCCGAGCCC ATCCCATGGC CCATGCTATA AATGCCGCCT CATCTGTCGG CTTGGTGAGC 
CAGCCACCGC CTGTGGCAAC GACTAATGGT AATCTGGCAG CTGCTGGTGT CTCGCCTCCT 
CCGCCGCTGG AAATGTATTC GGGTCGATCC ACCATTCCCT ACTGTGGCTC CCCGCCTTTC 
AGCGTGCGAC GGTCGGAGGC AGCGTACAGC AATCGGCAGC ATTATCAGCC GCAGCCGCAT 
CAAGGTCATC AGGCCCCACA AAATCAAATG CACGCGCATA TACATCCCCC TCATCGAGCA 
AGTGCCATTG TGGCCACCTC GCTGACACCG GCGCCGCCAT ATCCGGTACA TCAGAATCTG 
TGGTATCGCC AGCAGAGCAT GCAGGAAATG CACCGTCGCC ACATGACGCC CACGCCCATT 
GATCTCTCCT CGAATGCTCC CATTTTGACC AGCAGTCTGC GGACCCGCTA CCTGCATAGC 
ATTTGCAATT GCGTGCATGC CAGGAACGGC CCGGTCTCTA GCTTGGATCC GGCCTACTAT 
CCGCCCTACG ATGGCAATGC CAGTGCTGCG GCTCCGTCCC AGCAGATACC TTCCAGTGGT 
GGCCAGGCCA CGACCGTATC CGTTGCCCAG CCACAGCACT CTCAGTCAAA CTTGCGACAT 
ATGCAAGTGC AGCACCAGCA GCCTCAGGCC CAGCAGCAGT CCCCGCCGGT TCTGCAGCAT 
CTGCAGCGTC AGCGTGCGAT CCATCATCAT ATGTTCCACC ATCATTACTC GCCGCTGCAT 
TTGGAAATTG GACTTGCAAC GCCGCTGTCG CTCGGCTCAC GGATAGTGAT TGGAGCAGCA 
CGTCCAAATC GGGGGGCCAC TTTGGAAACA ATTGAGCGCA ATACCTTGCC CCACAAGTAC 
CGGCGTTTGC GTCGACCCAG CGAATCAGAC GAGGACGCGG AGAAGTGTGC TATATGCTTG 
TCTCTGTTCG AGATCGAGAA CGATGTGCGC CGCCTTCCCT GTATGCATCT ATTTCATACC 
GATTGCGTTG ATCAATGGTT AGTCACCAAT AAACACTGCC CTATTTGCCG AGTCGACATC 
GAGACCCACA TGGCGAATGA CGCACTCAAC TCCAGTGGGG TCACTGATGC CGCCAATGCC 
GCCGCCTTAT GACATTGTGT GCATGGAATC TTCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA19959 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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