Dpse\GA10288 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA10288
Entrez Gene ID	4801571
Full Name	GA10288 gene product from transcript GA10288-RB
Synonyms	GA10288,dpse_GLEANR_5509
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_003736501.2	XP_003736549.1	uncharacterized protein, isoform B
XM_001358605.3	XP_001358642.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA10288 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA10288 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe01177	XM_001358605.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10288), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe01176	XM_003736501.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10288), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA10288 ( XM_001358605.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001358642.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10288), mRNA.

Price & Availability↑

CloneID	ODe01177
Clone ID Related Accession (Same CDS sequence)	XM_001358605.3
Accession Version	XM_001358605.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001358605.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTACTG TGTGGAGCGA TTGCAATGCT AAGCAAGCAT TCGGCATTGC CAAGTGCAAT 
TTCGATCAGG AGAGGAAGCC GCATCGCTTG AACCTGGACG TGGGCGATGC AGTCATAATA 
CTCAAGGAGA CCACCCACTG GTACTACGGC TACAGGCAAA AAGCCAAGGA AACGCGCGGC 
ATATTTCCCA AGAGCTATGT GCATCTGTGC GAGTACAGCA TTGTCAATGG GGAGTTCTGC 
ATCCAGCGCA CAGACATTGT CGAGGAGATC ACCAAAGTGC TGCTGGAGTG GGGCGCCATA 
GCCAAGAGTT ATTATTTGGC CACAAATCCG AGCTTTCAAA AGATACGCCG CAAGATGAAC 
GAACTGAACA ACAATCGAGC GGCCTTGATC TCTGGCAACC TGCCTCTGGA TGAGGTGCGG 
AAAGTGAAGC TCTTGGCCAC GGCCCAGATC GATACAGGGA ACAAGATCCT CAGCCTGGAC 
ATGGTGGTGC GCGACGAGAG CGGGGACATA CTGGACACGA ACGCCATTTG CACCACAGAG 
CTGTACGAGC ACCATGTCCA CGCCGTCTCC CGCATAGAGA AGGCGAATCG TCTCTCAACG 
GAGCGTGGAA CCACCCGCAG CCTCAACAAA TACTCGCACA ACATCCTGCT GCATGTGAAT 
GCATTCGTGT GCAAGTTCCA AGAGGACGCG GACCTGCTCT TCACACTCTT CGATGGAGAG 
ACGCACAAAC CCATCAGCGA AAACTATGTC GTGAAGTGGT CGCGCACGGG CATTGCCCGG 
GACATCGAAC AGATCGACAA CAATCGGGTG CTCTTCACGG ATCTCAGCAG AAGCGACCTG 
AACATTGCCA AAATGTATCT GGTGTGCTAT GCCATACGCA TCGGGGCGAT GGAGGTCAAG 
GACTCCGCCG ACTCGAAGCG CACCAGCATG AGCATAGCCA ACAGTGTCCT CACAGCCGCC 
AGTCGGAAGC ACTCGCAGCT GTCTGTCAGC TCCAATGGAT CTGCGGGCAG CACCGGCGAG 
TACGTACTCC GACGGCCCTT TGGCGTCGCC TGCAAGGACC TGACGCCCAT CATTAGCAAA 
TCGGAGGACT ATCGGGGCAA CCTGGACCTG CCGTTCATCA TGTGCGAGAA GGACACGCTG 
GACGGGACGC TGCGGAAGCT GATTGCCAAC AAGGACATCG GGAAGATCGA CTCCAAGATG 
GCCGTGACCA TTGAGGTGCT GCGCGGCGAC ATCAAGCAGA TCAAAGAGGA CTTCCCCCGG 
CTGGTGCACA GCAACGTCCC AGTGGCCCGC AAGATGGGCT TCCCCGAGGT CATCCTCCCA 
GGGGATGTGC GCAACGATCT GTATCTGACG ATCTGCAGCG GAGAGTTCGC CAAGACGGCC 
AAGAACGTGG AGGTGTCCGT GTGCGTGGCC AACGAGCAGG GCAGCCTCAT GCCGGGAGTC 
ATCAGCATGG GCGCCGGCCA TCCGCCCATC GACGAGTACA AGTCGGTGGT GTACTACCAC 
GAGGACAAGC CGAAGTGGCA GGAGACCTTC AAGATTCACG TGCCCATCGA GGACTTCAAG 
CAGTGCCATT TGCGGTTCAC TCTGAAGCAC CGCAGCAGCA ACGAGCAGCG CGACAGGCAG 
GACAAGTCCT TCGTTCTGGC CTATGTGCGT CTCATGCAGG CCAACGGCAC GACCATTCCG 
CAGGGCCAGC ATGTTCTGGC CGTCTACAAG ATCGATCACA AGAAGTACGA CAAGTCGGAT 
GCCAATTGCT ACATGGAACT GCCGTCGACC CTGGCCGAGC TGCAGGGCAC CAGACCCTCC 
AACAGCGCCA TGTCGCTCCT GCCTAAGGAT CAGCTGACAA TTGGGGTGAA TCTATGCAGC 
ACAAAGCTCA CGCAGAGCGT GAGCCTTTTG GGTCTTCTGA ACTGGTCTGC GCACAAGGAA 
ACCCTCGAGC AGTCGCTCAA TGCCCTGTGC TCAGTGCCCG GCGAGGAGGT GGTCAAGTTT 
CTGCAGGACA TACTCGATGC CTTGTTCAAT ATTCTGGTGG AGAACGATCA TCCGGAGAAG 
TACGATCAGC TGGTGTTCAT GAGCATCATT CATCTGATCG AAACGGTGTC CGAGCTGAAG 
TACCAGCACT TTCTCACCGT CCTGGATGTC TACATCAACG AGAGCTTCTC CTTTACGCTG 
GCCTACACCA AACTTATGGA TGTGCTGCAG AAGAACATTA GCGATGCCAT TGCCCCCCGA 
GAGAAATCCG CGGATGCCGA AGAGAGTGCA GAGGTGCGGC GCCTGTACAA GACCACGCGC 
TACCTGCACT ACGTGATGAA GTTTGTGATC AGGTCGCGGG TGCTCTATGC GGAGATGAAC 
TGCAACACGG ACTATGTGGA CTTTGCCACG CGCCTGCAGG AGCTGTTGCG CATGTTCATC 
GACATGATTG GATGCCCGAG CAATCTGCTG AAGTCCGAGG GAGCCCTGCT CAAGAATCTG 
CACATCATAG CCACCGATCT GATGCAGGTC TTTGACCAAG TGCGATTGAG CGTTTCCATT 
GTGGAGATCT TGGAGAAGTT TCCGCCGCGT CGCTTGACGC AGTCCAAGAT GGGCTGCATC 
AAGGATTTCG TGGAGACGAA GCTCTTCAGT TCGCCCAAGT GTCGGGCCAT CCTGCTGCCG 
GTGTTCTGCA AGCACATCAA GGATCATCTC GAGAGCAAAG AGGAGATTGC CGAGTGCATC 
AACATCATGA ACAATATACT GAAGCTGCTG TACCGCACGG ATGTGGGAGC CACCCACAAT 
GACATTCGGG ACATCATGAT CATTCTGCTG CGCACCGTGA TGAAGGCGGC CCACTCGCTG 
GACCGGGACA CGGGGCTGGT GGGAAAGTTC TTTGCCATTA TGCTGGGCAT ACTGCAGCGC 
ATGGACGCCC AGCACTATGA GTACTTTGTC AAGGATCTGC ATCAGAGGGG GGAGCTCAAG 
GACTTTGTAA TCGAAATCCT GCTGGTGTTC GAGGAGCTGG TGTCGCCGCA CCAGAAGGCA 
GTGTTTCCCA GGGACTGGAT GGACATGATC ATGCACCAGA ACACGGTCAT CCTGGGCGCC 
CTAAAGCACC TGAGCGTTGT CATAACCGAC TACTTCCTGT GTCCCTTCGA GAAGCAGATC 
TGGTCAAACT TCTTCCAGTG CTCCATCGCC TTCCTAGTGC AGTCGCCGCT CCAGCTGAAC 
GACTTCAACG ACAACAAGCG GCAGATTGTG TTCGCCCGCT ATCGCGACAT TCGCAAGGAC 
ACGGCCATGG AGATACGCAA GATGTGGTTC CAGCTGGGCC AGCACAAGCC CAAGTTTGTG 
CCGCAGCTGG TCGAACCGAT TCTGGAGATG AGCATGATAC CGGAGACGGA GCTGCGGCGC 
GAGACGATCC CCATCTTCTT CGACATGATG CAGTGCGAGT ACTACAGCTC GCGGCTGGAG 
CTGGAGAGCT ATGGCGACAC CAAATACAAC AATGCCCACC ACAAGGGAAA CTTTACGGAG 
TTCAAGACGG CCATGATCGA GAAGCTGGAC ATCCTCATTG GAGCTGGCAA GGGCGATGCC 
GAGTACAAGC AGATGTTCGA GGAGATCATG CTGGAGCGCT GTGCCGCCCA CAACACTTTG 
AATGTGGATG GGACCAGCTT TGTGCAGATG GTAACGCGTC TGATGGACAA GCTGCTGGAG 
TATCGCTTCA TCATCCAGGA CGAGAGCAAG GAGAACCGCA TGGCCTGCAC CTTCTCGCTG 
CTGCAGTTCT ACTCGGAGGT GGACCTCAAG GAGATGTACA TCCGCTACGT CTACAAGCTG 
CGCGACCTGC ACATGGAGTT CGACAACTAC ACGGAGGCCG CCTTCACGCT GAAGCTGCAC 
ACCGAACTGC TGCGCTGGAC CGACATGGAG CTGTCGCCGC AGTTGCGCAG CTATCGGCAC 
AGCCTGTGCC GCACCCACAG GGACCTGAAG GAGGCCCTGT ACTTTGATAT CCTGGAGTAT 
TTTGACAAGG GCAAGCAGTG GGAGTGCGCC ATCGACATGT GCAAGGTGCT GGCCCGCCAA 
TACGAGGAGG AGATCTACGA CTACATCAAA CTGTCGGAGC TGCTGAAGCG CATGGCGCAG 
TTTTACGAAA AGATCCTCAA GGAGCTGCGC CACAGCTGCG AGTACTTCCG CGTGTGCTTC 
TATGGGCGCA AGTTCCCGCG CCTCCTCCAG AACCGGGTGT ACATCTTCCG GGGCAAGGAG 
TACGAGCGGC ACAGCGACTT CTGCACTCGC ATGCTGCAGC AGCATCCGCA GGCGGAGCTC 
ATGCAAACAC TGGAGGCGCC AGGGGAGGAC ATCACACAAA GCGATGCCCA GTACATCCAA 
GTCAACAAGG TGGAGCCCAT TATGGACGCG GCCTTTAGCA AGTTCCACGA CAAGATCATT 
TCCAACGAGA TCGTCAAGTA CTACACCGCC AACAATGTGC AGAAGTTCCG GTTCTCGCGA 
CCCTTCCGCG ACAGCAGCGG CGGCGGCGAT GATGTGCGGA ACCTTTGGCT CGAGCGCACG 
GAGCTGATCA CGCGCTTCCC CCTGCCCGGC ATCCTGCGCT GGTTCCCCGT CGTCGAGTCC 
ATTACGTTCA AGATTTCCCC TCTAGAGCGT GCAGTGGAGA TCATGAAGGA CACGAACCGG 
GACATTAGGC AGCTGGTAAT CCTGCACAAA AGCGACGAGA CGCTGCACAT CAATCCGCTG 
AGCATGAAGC TCAATGGGAT CGTGGATCCG GCCGTGATGG GAGGCTTCGC CAAGTACGAG 
GAGGCCTTCC TGACCGACGA GTATCTGGAG GAGCATCCCG ATGACAAGGA TCTGATCGAG 
GAGCTCAAAG AACTGATTGC CACGCAGATA CCGCTGCTGG AGATAGCCAT TCTGCTGCAC 
CGCCAGAAGG CGCCCGATAG CCTAAAGGCT ATGCAGGAGC ACCTGGAGAG CTGCTTCTCG 
GACATGCAGC AGCACGTGGA GACGCGCTAC GGCCGGAAGA CCTGTGACCT GAAGATTGAA 
CGCGATTCTG TGGTGATGCG ACGCACCAAC TCCTTTCTGC CGGCCCTCTT CGATGGCGGC 
TGCAATCGTT TGTCGGAGAC CAGCATGGGA TCCTCAGACA GCGGACTGTC AAAGTCAACC 
ATTCTGCCCA GGCCGCCGAC CAACTCCATC AAGTCCACGC TGGCCAGCCT GAGTTTCAAC 
ACGAGGCCCA GCTTGGGACA CTCGCCAAGC ACCAAGAGCA GCAAGAACAA GGACAAGACA 
CCAGCCAAGC GGCGGTCTAT GAAGAAGGAT CGTGAGGCGC TTAGCTTTCC CAGCAGTCAA 
TGGTATACGC CGCCATTGAC CACCATTACC TCGACGCCCG AGAAGGAGAT CAACACGTCG 
ATAGGCTCGC TGGCCAGCGC TTCCAACAGC TCGTTGAGTG GCCCGAAAAC ACCAGATCCT 
CATGTGCTGA CCGAGGAGCT CACACCAAAG CGGCCTCTGC GCTCCGAAAA GGAGAAGGAA 
CGTCGCCTCT CTAGGCCTGC CAGCATTGCC ACGCCCACGG CCAGCATCAA GAACTTCCCG 
AACGACACAC GCTCCCTATC CGAGAGCAGT AATCGGAATT CAGTTGAAAC CACAGATTCC 
ACGTCGGAGG AGGACATTAG ACCGCCGCCA TTGCCGGCCA AGACACGCGA CTCGACCGAT 
TTCACAAGCC TGTCACACAA CCTGGACTGG ACACCGAATA GCTATGCGAT GCTCAGCACT 
CAGACCAGCT TCACCAGCAA CAGCAGCAGC ACGAGCACGA CAATGACAAA GACCAGTATT 
ACAAACAGCA CGTACGAGTA TCTGGAGGCC ACGAACTTTA GCCTGGTGGG TGCCGGCGCC 
GCCTCACCAG ATGCCAGTAA GCCGCGTCCC CCAACGCCGC CGCCGAAGCC ATCGCGGCAC 
AGCAAACACA TTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001358642.2
CDS	468..6365
Translation	MTTVWSDCNAKQAFGIAKCNFDQERKPHRLNLDVGDAVIILKETTHWYYGYRQKAKETRGIFPKSYVHLCEYSIVNGEFCIQRTDIVEEITKVLLEWGAIAKSYYLATNPSFQKIRRKMNELNNNRAALISGNLPLDEVRKVKLLATAQIDTGNKILSLDMVVRDESGDILDTNAICTTELYEHHVHAVSRIEKANRLSTERGTTRSLNKYSHNILLHVNAFVCKFQEDADLLFTLFDGETHKPISENYVVKWSRTGIARDIEQIDNNRVLFTDLSRSDLNIAKMYLVCYAIRIGAMEVKDSADSKRTSMSIANSVLTAASRKHSQLSVSSNGSAGSTGEYVLRRPFGVACKDLTPIISKSEDYRGNLDLPFIMCEKDTLDGTLRKLIANKDIGKIDSKMAVTIEVLRGDIKQIKEDFPRLVHSNVPVARKMGFPEVILPGDVRNDLYLTICSGEFAKTAKNVEVSVCVANEQGSLMPGVISMGAGHPPIDEYKSVVYYHEDKPKWQETFKIHVPIEDFKQCHLRFTLKHRSSNEQRDRQDKSFVLAYVRLMQANGTTIPQGQHVLAVYKIDHKKYDKSDANCYMELPSTLAELQGTRPSNSAMSLLPKDQLTIGVNLCSTKLTQSVSLLGLLNWSAHKETLEQSLNALCSVPGEEVVKFLQDILDALFNILVENDHPEKYDQLVFMSIIHLIETVSELKYQHFLTVLDVYINESFSFTLAYTKLMDVLQKNISDAIAPREKSADAEESAEVRRLYKTTRYLHYVMKFVIRSRVLYAEMNCNTDYVDFATRLQELLRMFIDMIGCPSNLLKSEGALLKNLHIIATDLMQVFDQVRLSVSIVEILEKFPPRRLTQSKMGCIKDFVETKLFSSPKCRAILLPVFCKHIKDHLESKEEIAECINIMNNILKLLYRTDVGATHNDIRDIMIILLRTVMKAAHSLDRDTGLVGKFFAIMLGILQRMDAQHYEYFVKDLHQRGELKDFVIEILLVFEELVSPHQKAVFPRDWMDMIMHQNTVILGALKHLSVVITDYFLCPFEKQIWSNFFQCSIAFLVQSPLQLNDFNDNKRQIVFARYRDIRKDTAMEIRKMWFQLGQHKPKFVPQLVEPILEMSMIPETELRRETIPIFFDMMQCEYYSSRLELESYGDTKYNNAHHKGNFTEFKTAMIEKLDILIGAGKGDAEYKQMFEEIMLERCAAHNTLNVDGTSFVQMVTRLMDKLLEYRFIIQDESKENRMACTFSLLQFYSEVDLKEMYIRYVYKLRDLHMEFDNYTEAAFTLKLHTELLRWTDMELSPQLRSYRHSLCRTHRDLKEALYFDILEYFDKGKQWECAIDMCKVLARQYEEEIYDYIKLSELLKRMAQFYEKILKELRHSCEYFRVCFYGRKFPRLLQNRVYIFRGKEYERHSDFCTRMLQQHPQAELMQTLEAPGEDITQSDAQYIQVNKVEPIMDAAFSKFHDKIISNEIVKYYTANNVQKFRFSRPFRDSSGGGDDVRNLWLERTELITRFPLPGILRWFPVVESITFKISPLERAVEIMKDTNRDIRQLVILHKSDETLHINPLSMKLNGIVDPAVMGGFAKYEEAFLTDEYLEEHPDDKDLIEELKELIATQIPLLEIAILLHRQKAPDSLKAMQEHLESCFSDMQQHVETRYGRKTCDLKIERDSVVMRRTNSFLPALFDGGCNRLSETSMGSSDSGLSKSTILPRPPTNSIKSTLASLSFNTRPSLGHSPSTKSSKNKDKTPAKRRSMKKDREALSFPSSQWYTPPLTTITSTPEKEINTSIGSLASASNSSLSGPKTPDPHVLTEELTPKRPLRSEKEKERRLSRPASIATPTASIKNFPNDTRSLSESSNRNSVETTDSTSEEDIRPPPLPAKTRDSTDFTSLSHNLDWTPNSYAMLSTQTSFTSNSSSTSTTMTKTSITNSTYEYLEATNFSLVGAGAASPDASKPRPPTPPPKPSRHSKHIP

Target ORF information:

RefSeq Version	XM_001358605.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA10288), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001358605.3

ORF Insert Sequence:

ATGACTACTG TGTGGAGCGA TTGCAATGCT AAGCAAGCAT TCGGCATTGC CAAGTGCAAT 
TTCGATCAGG AGAGGAAGCC GCATCGCTTG AACCTGGACG TGGGCGATGC AGTCATAATA 
CTCAAGGAGA CCACCCACTG GTACTACGGC TACAGGCAAA AAGCCAAGGA AACGCGCGGC 
ATATTTCCCA AGAGCTATGT GCATCTGTGC GAGTACAGCA TTGTCAATGG GGAGTTCTGC 
ATCCAGCGCA CAGACATTGT CGAGGAGATC ACCAAAGTGC TGCTGGAGTG GGGCGCCATA 
GCCAAGAGTT ATTATTTGGC CACAAATCCG AGCTTTCAAA AGATACGCCG CAAGATGAAC 
GAACTGAACA ACAATCGAGC GGCCTTGATC TCTGGCAACC TGCCTCTGGA TGAGGTGCGG 
AAAGTGAAGC TCTTGGCCAC GGCCCAGATC GATACAGGGA ACAAGATCCT CAGCCTGGAC 
ATGGTGGTGC GCGACGAGAG CGGGGACATA CTGGACACGA ACGCCATTTG CACCACAGAG 
CTGTACGAGC ACCATGTCCA CGCCGTCTCC CGCATAGAGA AGGCGAATCG TCTCTCAACG 
GAGCGTGGAA CCACCCGCAG CCTCAACAAA TACTCGCACA ACATCCTGCT GCATGTGAAT 
GCATTCGTGT GCAAGTTCCA AGAGGACGCG GACCTGCTCT TCACACTCTT CGATGGAGAG 
ACGCACAAAC CCATCAGCGA AAACTATGTC GTGAAGTGGT CGCGCACGGG CATTGCCCGG 
GACATCGAAC AGATCGACAA CAATCGGGTG CTCTTCACGG ATCTCAGCAG AAGCGACCTG 
AACATTGCCA AAATGTATCT GGTGTGCTAT GCCATACGCA TCGGGGCGAT GGAGGTCAAG 
GACTCCGCCG ACTCGAAGCG CACCAGCATG AGCATAGCCA ACAGTGTCCT CACAGCCGCC 
AGTCGGAAGC ACTCGCAGCT GTCTGTCAGC TCCAATGGAT CTGCGGGCAG CACCGGCGAG 
TACGTACTCC GACGGCCCTT TGGCGTCGCC TGCAAGGACC TGACGCCCAT CATTAGCAAA 
TCGGAGGACT ATCGGGGCAA CCTGGACCTG CCGTTCATCA TGTGCGAGAA GGACACGCTG 
GACGGGACGC TGCGGAAGCT GATTGCCAAC AAGGACATCG GGAAGATCGA CTCCAAGATG 
GCCGTGACCA TTGAGGTGCT GCGCGGCGAC ATCAAGCAGA TCAAAGAGGA CTTCCCCCGG 
CTGGTGCACA GCAACGTCCC AGTGGCCCGC AAGATGGGCT TCCCCGAGGT CATCCTCCCA 
GGGGATGTGC GCAACGATCT GTATCTGACG ATCTGCAGCG GAGAGTTCGC CAAGACGGCC 
AAGAACGTGG AGGTGTCCGT GTGCGTGGCC AACGAGCAGG GCAGCCTCAT GCCGGGAGTC 
ATCAGCATGG GCGCCGGCCA TCCGCCCATC GACGAGTACA AGTCGGTGGT GTACTACCAC 
GAGGACAAGC CGAAGTGGCA GGAGACCTTC AAGATTCACG TGCCCATCGA GGACTTCAAG 
CAGTGCCATT TGCGGTTCAC TCTGAAGCAC CGCAGCAGCA ACGAGCAGCG CGACAGGCAG 
GACAAGTCCT TCGTTCTGGC CTATGTGCGT CTCATGCAGG CCAACGGCAC GACCATTCCG 
CAGGGCCAGC ATGTTCTGGC CGTCTACAAG ATCGATCACA AGAAGTACGA CAAGTCGGAT 
GCCAATTGCT ACATGGAACT GCCGTCGACC CTGGCCGAGC TGCAGGGCAC CAGACCCTCC 
AACAGCGCCA TGTCGCTCCT GCCTAAGGAT CAGCTGACAA TTGGGGTGAA TCTATGCAGC 
ACAAAGCTCA CGCAGAGCGT GAGCCTTTTG GGTCTTCTGA ACTGGTCTGC GCACAAGGAA 
ACCCTCGAGC AGTCGCTCAA TGCCCTGTGC TCAGTGCCCG GCGAGGAGGT GGTCAAGTTT 
CTGCAGGACA TACTCGATGC CTTGTTCAAT ATTCTGGTGG AGAACGATCA TCCGGAGAAG 
TACGATCAGC TGGTGTTCAT GAGCATCATT CATCTGATCG AAACGGTGTC CGAGCTGAAG 
TACCAGCACT TTCTCACCGT CCTGGATGTC TACATCAACG AGAGCTTCTC CTTTACGCTG 
GCCTACACCA AACTTATGGA TGTGCTGCAG AAGAACATTA GCGATGCCAT TGCCCCCCGA 
GAGAAATCCG CGGATGCCGA AGAGAGTGCA GAGGTGCGGC GCCTGTACAA GACCACGCGC 
TACCTGCACT ACGTGATGAA GTTTGTGATC AGGTCGCGGG TGCTCTATGC GGAGATGAAC 
TGCAACACGG ACTATGTGGA CTTTGCCACG CGCCTGCAGG AGCTGTTGCG CATGTTCATC 
GACATGATTG GATGCCCGAG CAATCTGCTG AAGTCCGAGG GAGCCCTGCT CAAGAATCTG 
CACATCATAG CCACCGATCT GATGCAGGTC TTTGACCAAG TGCGATTGAG CGTTTCCATT 
GTGGAGATCT TGGAGAAGTT TCCGCCGCGT CGCTTGACGC AGTCCAAGAT GGGCTGCATC 
AAGGATTTCG TGGAGACGAA GCTCTTCAGT TCGCCCAAGT GTCGGGCCAT CCTGCTGCCG 
GTGTTCTGCA AGCACATCAA GGATCATCTC GAGAGCAAAG AGGAGATTGC CGAGTGCATC 
AACATCATGA ACAATATACT GAAGCTGCTG TACCGCACGG ATGTGGGAGC CACCCACAAT 
GACATTCGGG ACATCATGAT CATTCTGCTG CGCACCGTGA TGAAGGCGGC CCACTCGCTG 
GACCGGGACA CGGGGCTGGT GGGAAAGTTC TTTGCCATTA TGCTGGGCAT ACTGCAGCGC 
ATGGACGCCC AGCACTATGA GTACTTTGTC AAGGATCTGC ATCAGAGGGG GGAGCTCAAG 
GACTTTGTAA TCGAAATCCT GCTGGTGTTC GAGGAGCTGG TGTCGCCGCA CCAGAAGGCA 
GTGTTTCCCA GGGACTGGAT GGACATGATC ATGCACCAGA ACACGGTCAT CCTGGGCGCC 
CTAAAGCACC TGAGCGTTGT CATAACCGAC TACTTCCTGT GTCCCTTCGA GAAGCAGATC 
TGGTCAAACT TCTTCCAGTG CTCCATCGCC TTCCTAGTGC AGTCGCCGCT CCAGCTGAAC 
GACTTCAACG ACAACAAGCG GCAGATTGTG TTCGCCCGCT ATCGCGACAT TCGCAAGGAC 
ACGGCCATGG AGATACGCAA GATGTGGTTC CAGCTGGGCC AGCACAAGCC CAAGTTTGTG 
CCGCAGCTGG TCGAACCGAT TCTGGAGATG AGCATGATAC CGGAGACGGA GCTGCGGCGC 
GAGACGATCC CCATCTTCTT CGACATGATG CAGTGCGAGT ACTACAGCTC GCGGCTGGAG 
CTGGAGAGCT ATGGCGACAC CAAATACAAC AATGCCCACC ACAAGGGAAA CTTTACGGAG 
TTCAAGACGG CCATGATCGA GAAGCTGGAC ATCCTCATTG GAGCTGGCAA GGGCGATGCC 
GAGTACAAGC AGATGTTCGA GGAGATCATG CTGGAGCGCT GTGCCGCCCA CAACACTTTG 
AATGTGGATG GGACCAGCTT TGTGCAGATG GTAACGCGTC TGATGGACAA GCTGCTGGAG 
TATCGCTTCA TCATCCAGGA CGAGAGCAAG GAGAACCGCA TGGCCTGCAC CTTCTCGCTG 
CTGCAGTTCT ACTCGGAGGT GGACCTCAAG GAGATGTACA TCCGCTACGT CTACAAGCTG 
CGCGACCTGC ACATGGAGTT CGACAACTAC ACGGAGGCCG CCTTCACGCT GAAGCTGCAC 
ACCGAACTGC TGCGCTGGAC CGACATGGAG CTGTCGCCGC AGTTGCGCAG CTATCGGCAC 
AGCCTGTGCC GCACCCACAG GGACCTGAAG GAGGCCCTGT ACTTTGATAT CCTGGAGTAT 
TTTGACAAGG GCAAGCAGTG GGAGTGCGCC ATCGACATGT GCAAGGTGCT GGCCCGCCAA 
TACGAGGAGG AGATCTACGA CTACATCAAA CTGTCGGAGC TGCTGAAGCG CATGGCGCAG 
TTTTACGAAA AGATCCTCAA GGAGCTGCGC CACAGCTGCG AGTACTTCCG CGTGTGCTTC 
TATGGGCGCA AGTTCCCGCG CCTCCTCCAG AACCGGGTGT ACATCTTCCG GGGCAAGGAG 
TACGAGCGGC ACAGCGACTT CTGCACTCGC ATGCTGCAGC AGCATCCGCA GGCGGAGCTC 
ATGCAAACAC TGGAGGCGCC AGGGGAGGAC ATCACACAAA GCGATGCCCA GTACATCCAA 
GTCAACAAGG TGGAGCCCAT TATGGACGCG GCCTTTAGCA AGTTCCACGA CAAGATCATT 
TCCAACGAGA TCGTCAAGTA CTACACCGCC AACAATGTGC AGAAGTTCCG GTTCTCGCGA 
CCCTTCCGCG ACAGCAGCGG CGGCGGCGAT GATGTGCGGA ACCTTTGGCT CGAGCGCACG 
GAGCTGATCA CGCGCTTCCC CCTGCCCGGC ATCCTGCGCT GGTTCCCCGT CGTCGAGTCC 
ATTACGTTCA AGATTTCCCC TCTAGAGCGT GCAGTGGAGA TCATGAAGGA CACGAACCGG 
GACATTAGGC AGCTGGTAAT CCTGCACAAA AGCGACGAGA CGCTGCACAT CAATCCGCTG 
AGCATGAAGC TCAATGGGAT CGTGGATCCG GCCGTGATGG GAGGCTTCGC CAAGTACGAG 
GAGGCCTTCC TGACCGACGA GTATCTGGAG GAGCATCCCG ATGACAAGGA TCTGATCGAG 
GAGCTCAAAG AACTGATTGC CACGCAGATA CCGCTGCTGG AGATAGCCAT TCTGCTGCAC 
CGCCAGAAGG CGCCCGATAG CCTAAAGGCT ATGCAGGAGC ACCTGGAGAG CTGCTTCTCG 
GACATGCAGC AGCACGTGGA GACGCGCTAC GGCCGGAAGA CCTGTGACCT GAAGATTGAA 
CGCGATTCTG TGGTGATGCG ACGCACCAAC TCCTTTCTGC CGGCCCTCTT CGATGGCGGC 
TGCAATCGTT TGTCGGAGAC CAGCATGGGA TCCTCAGACA GCGGACTGTC AAAGTCAACC 
ATTCTGCCCA GGCCGCCGAC CAACTCCATC AAGTCCACGC TGGCCAGCCT GAGTTTCAAC 
ACGAGGCCCA GCTTGGGACA CTCGCCAAGC ACCAAGAGCA GCAAGAACAA GGACAAGACA 
CCAGCCAAGC GGCGGTCTAT GAAGAAGGAT CGTGAGGCGC TTAGCTTTCC CAGCAGTCAA 
TGGTATACGC CGCCATTGAC CACCATTACC TCGACGCCCG AGAAGGAGAT CAACACGTCG 
ATAGGCTCGC TGGCCAGCGC TTCCAACAGC TCGTTGAGTG GCCCGAAAAC ACCAGATCCT 
CATGTGCTGA CCGAGGAGCT CACACCAAAG CGGCCTCTGC GCTCCGAAAA GGAGAAGGAA 
CGTCGCCTCT CTAGGCCTGC CAGCATTGCC ACGCCCACGG CCAGCATCAA GAACTTCCCG 
AACGACACAC GCTCCCTATC CGAGAGCAGT AATCGGAATT CAGTTGAAAC CACAGATTCC 
ACGTCGGAGG AGGACATTAG ACCGCCGCCA TTGCCGGCCA AGACACGCGA CTCGACCGAT 
TTCACAAGCC TGTCACACAA CCTGGACTGG ACACCGAATA GCTATGCGAT GCTCAGCACT 
CAGACCAGCT TCACCAGCAA CAGCAGCAGC ACGAGCACGA CAATGACAAA GACCAGTATT 
ACAAACAGCA CGTACGAGTA TCTGGAGGCC ACGAACTTTA GCCTGGTGGG TGCCGGCGCC 
GCCTCACCAG ATGCCAGTAA GCCGCGTCCC CCAACGCCGC CGCCGAAGCC ATCGCGGCAC 
AGCAAACACA TTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA10288 ( XM_003736501.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_003736549.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10288), mRNA.

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CloneID	ODe01176
Clone ID Related Accession (Same CDS sequence)	XM_003736501.2
Accession Version	XM_003736501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_003736501.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTACTG TGTGGAGCGA TTGCAATGCT AAGCAAGCAT TCGGCATTGC CAAGTGCAAT 
TTCGATCAGG AGAGGAAGCC GCATCGCTTG AACCTGGACG TGGGCGATGC AGTCATAATA 
CTCAAGGAGA CCACCCACTG GTACTACGGC TACAGGCAAA AAGCCAAGGA AACGCGCGGC 
ATATTTCCCA AGAGCTATGT GCATCTGTGC GAGTACAGCA TTGTCAATGG GGAGTTCTGC 
ATCCAGCGCA CAGACATTGT CGAGGAGATC ACCAAAGTGC TGCTGGAGTG GGGCGCCATA 
GCCAAGAGTT ATTATTTGGC CACAAATCCG AGCTTTCAAA AGATACGCCG CAAGATGAAC 
GAACTGAACA ACAATCGAGC GGCCTTGATC TCTGGCAACC TGCCTCTGGA TGAGGTGCGG 
AAAGTGAAGC TCTTGGCCAC GGCCCAGATC GATACAGGGA ACAAGATCCT CAGCCTGGAC 
ATGGTGGTGC GCGACGAGAG CGGGGACATA CTGGACACGA ACGCCATTTG CACCACAGAG 
CTGTACGAGC ACCATGTCCA CGCCGTCTCC CGCATAGAGA AGGCGAATCG TCTCTCAACG 
GAGCGTGGAA CCACCCGCAG CCTCAACAAA TACTCGCACA ACATCCTGCT GCATGTGAAT 
GCATTCGTGT GCAAGTTCCA AGAGGACGCG GACCTGCTCT TCACACTCTT CGATGGAGAG 
ACGCACAAAC CCATCAGCGA AAACTATGTC GTGAAGTGGT CGCGCACGGG CATTGCCCGG 
GACATCGAAC AGATCGACAA CAATCGGGTG CTCTTCACGG ATCTCAGCAG AAGCGACCTG 
AACATTGCCA AAATGTATCT GGTGTGCTAT GCCATACGCA TCGGGGCGAT GGAGGTCAAG 
GACTCCGCCG ACTCGAAGCG CACCAGCATG AGCATAGCCA ACAGTGTCCT CACAGCCGCC 
AGTCGGAAGC ACTCGCAGCT GTCTGTCAGC TCCAATGGAT CTGCGGGCAG CACCGGCGAG 
TACGTACTCC GACGGCCCTT TGGCGTCGCC TGCAAGGACC TGACGCCCAT CATTAGCAAA 
TCGGAGGACT ATCGGGGCAA CCTGGACCTG CCGTTCATCA TGTGCGAGAA GGACACGCTG 
GACGGGACGC TGCGGAAGCT GATTGCCAAC AAGGACATCG GGAAGATCGA CTCCAAGATG 
GCCGTGACCA TTGAGGTGCT GCGCGGCGAC ATCAAGCAGA TCAAAGAGGA CTTCCCCCGG 
CTGGTGCACA GCAACGTCCC AGTGGCCCGC AAGATGGGCT TCCCCGAGGT CATCCTCCCA 
GGGGATGTGC GCAACGATCT GTATCTGACG ATCTGCAGCG GAGAGTTCGC CAAGACGGCC 
AAGAACGTGG AGGTGTCCGT GTGCGTGGCC AACGAGCAGG GCAGCCTCAT GCCGGGAGTC 
ATCAGCATGG GCGCCGGCCA TCCGCCCATC GACGAGTACA AGTCGGTGGT GTACTACCAC 
GAGGACAAGC CGAAGTGGCA GGAGACCTTC AAGATTCACG TGCCCATCGA GGACTTCAAG 
CAGTGCCATT TGCGGTTCAC TCTGAAGCAC CGCAGCAGCA ACGAGCAGCG CGACAGGCAG 
GACAAGTCCT TCGTTCTGGC CTATGTGCGT CTCATGCAGG CCAACGGCAC GACCATTCCG 
CAGGGCCAGC ATGTTCTGGC CGTCTACAAG ATCGATCACA AGAAGTACGA CAAGTCGGAT 
GCCAATTGCT ACATGGAACT GCCGTCGACC CTGGCCGAGC TGCAGGGCAC CAGACCCTCC 
AACAGCGCCA TGTCGCTCCT GCCTAAGGAT CAGCTGACAA TTGGGGTGAA TCTATGCAGC 
ACAAAGCTCA CGCAGAGCGT GAGCCTTTTG GGTCTTCTGA ACTGGTCTGC GCACAAGGAA 
ACCCTCGAGC AGTCGCTCAA TGCCCTGTGC TCAGTGCCCG GCGAGGAGGT GGTCAAGTTT 
CTGCAGGACA TACTCGATGC CTTGTTCAAT ATTCTGGTGG AGAACGATCA TCCGGAGAAG 
TACGATCAGC TGGTGTTCAT GAGCATCATT CATCTGATCG AAACGGTGTC CGAGCTGAAG 
TACCAGCACT TTCTCACCGT CCTGGATGTC TACATCAACG AGAGCTTCTC CTTTACGCTG 
GCCTACACCA AACTTATGGA TGTGCTGCAG AAGAACATTA GCGATGCCAT TGCCCCCCGA 
GAGAAATCCG CGGATGCCGA AGAGAGTGCA GAGGTGCGGC GCCTGTACAA GACCACGCGC 
TACCTGCACT ACGTGATGAA GTTTGTGATC AGGTCGCGGG TGCTCTATGC GGAGATGAAC 
TGCAACACGG ACTATGTGGA CTTTGCCACG CGCCTGCAGG AGCTGTTGCG CATGTTCATC 
GACATGATTG GATGCCCGAG CAATCTGCTG AAGTCCGAGG GAGCCCTGCT CAAGAATCTG 
CACATCATAG CCACCGATCT GATGCAGGTC TTTGACCAAG TGCGATTGAG CGTTTCCATT 
GTGGAGATCT TGGAGAAGTT TCCGCCGCGT CGCTTGACGC AGTCCAAGAT GGGCTGCATC 
AAGGATTTCG TGGAGACGAA GCTCTTCAGT TCGCCCAAGT GTCGGGCCAT CCTGCTGCCG 
GTGTTCTGCA AGCACATCAA GGATCATCTC GAGAGCAAAG AGGAGGGAGA CACGAAAACC 
GATATCTGGC AGCAAGAAAA GAATCTTTCG AAAGCCGCCA AAGTGCTAGG ACAGTCGAAA 
TCCCAACTGC ACACGCGTGA AACGACTGCC AACAAAAAGA TTGCCGAGTG CATCAACATC 
ATGAACAATA TACTGAAGCT GCTGTACCGC ACGGATGTGG GAGCCACCCA CAATGACATT 
CGGGACATCA TGATCATTCT GCTGCGCACC GTGATGAAGG CGGCCCACTC GCTGGACCGG 
GACACGGGGC TGGTGGGAAA GTTCTTTGCC ATTATGCTGG GCATACTGCA GCGCATGGAC 
GCCCAGCACT ATGAGTACTT TGTCAAGGAT CTGCATCAGA GGGGGGAGCT CAAGGACTTT 
GTAATCGAAA TCCTGCTGGT GTTCGAGGAG CTGGTGTCGC CGCACCAGAA GGCAGTGTTT 
CCCAGGGACT GGATGGACAT GATCATGCAC CAGAACACGG TCATCCTGGG CGCCCTAAAG 
CACCTGAGCG TTGTCATAAC CGACTACTTC CTGTGTCCCT TCGAGAAGCA GATCTGGTCA 
AACTTCTTCC AGTGCTCCAT CGCCTTCCTA GTGCAGTCGC CGCTCCAGCT GAACGACTTC 
AACGACAACA AGCGGCAGAT TGTGTTCGCC CGCTATCGCG ACATTCGCAA GGACACGGCC 
ATGGAGATAC GCAAGATGTG GTTCCAGCTG GGCCAGCACA AGCCCAAGTT TGTGCCGCAG 
CTGGTCGAAC CGATTCTGGA GATGAGCATG ATACCGGAGA CGGAGCTGCG GCGCGAGACG 
ATCCCCATCT TCTTCGACAT GATGCAGTGC GAGTACTACA GCTCGCGGCT GGAGCTGGAG 
AGCTATGGCG ACACCAAATA CAACAATGCC CACCACAAGG GAAACTTTAC GGAGTTCAAG 
ACGGCCATGA TCGAGAAGCT GGACATCCTC ATTGGAGCTG GCAAGGGCGA TGCCGAGTAC 
AAGCAGATGT TCGAGGAGAT CATGCTGGAG CGCTGTGCCG CCCACAACAC TTTGAATGTG 
GATGGGACCA GCTTTGTGCA GATGGTAACG CGTCTGATGG ACAAGCTGCT GGAGTATCGC 
TTCATCATCC AGGACGAGAG CAAGGAGAAC CGCATGGCCT GCACCTTCTC GCTGCTGCAG 
TTCTACTCGG AGGTGGACCT CAAGGAGATG TACATCCGCT ACGTCTACAA GCTGCGCGAC 
CTGCACATGG AGTTCGACAA CTACACGGAG GCCGCCTTCA CGCTGAAGCT GCACACCGAA 
CTGCTGCGCT GGACCGACAT GGAGCTGTCG CCGCAGTTGC GCAGCTATCG GCACAGCCTG 
TGCCGCACCC ACAGGGACCT GAAGGAGGCC CTGTACTTTG ATATCCTGGA GTATTTTGAC 
AAGGGCAAGC AGTGGGAGTG CGCCATCGAC ATGTGCAAGG TGCTGGCCCG CCAATACGAG 
GAGGAGATCT ACGACTACAT CAAACTGTCG GAGCTGCTGA AGCGCATGGC GCAGTTTTAC 
GAAAAGATCC TCAAGGAGCT GCGCCACAGC TGCGAGTACT TCCGCGTGTG CTTCTATGGG 
CGCAAGTTCC CGCGCCTCCT CCAGAACCGG GTGTACATCT TCCGGGGCAA GGAGTACGAG 
CGGCACAGCG ACTTCTGCAC TCGCATGCTG CAGCAGCATC CGCAGGCGGA GCTCATGCAA 
ACACTGGAGG CGCCAGGGGA GGACATCACA CAAAGCGATG CCCAGTACAT CCAAGTCAAC 
AAGGTGGAGC CCATTATGGA CGCGGCCTTT AGCAAGTTCC ACGACAAGAT CATTTCCAAC 
GAGATCGTCA AGTACTACAC CGCCAACAAT GTGCAGAAGT TCCGGTTCTC GCGACCCTTC 
CGCGACAGCA GCGGCGGCGG CGATGATGTG CGGAACCTTT GGCTCGAGCG CACGGAGCTG 
ATCACGCGCT TCCCCCTGCC CGGCATCCTG CGCTGGTTCC CCGTCGTCGA GTCCATTACG 
TTCAAGATTT CCCCTCTAGA GCGTGCAGTG GAGATCATGA AGGACACGAA CCGGGACATT 
AGGCAGCTGG TAATCCTGCA CAAAAGCGAC GAGACGCTGC ACATCAATCC GCTGAGCATG 
AAGCTCAATG GGATCGTGGA TCCGGCCGTG ATGGGAGGCT TCGCCAAGTA CGAGGAGGCC 
TTCCTGACCG ACGAGTATCT GGAGGAGCAT CCCGATGACA AGGATCTGAT CGAGGAGCTC 
AAAGAACTGA TTGCCACGCA GATACCGCTG CTGGAGATAG CCATTCTGCT GCACCGCCAG 
AAGGCGCCCG ATAGCCTAAA GGCTATGCAG GAGCACCTGG AGAGCTGCTT CTCGGACATG 
CAGCAGCACG TGGAGACGCG CTACGGCCGG AAGACCTGTG ACCTGAAGAT TGAACGCGAT 
TCTGTGGTGA TGCGACGCAC CAACTCCTTT CTGCCGGCCC TCTTCGATGG CGGCTGCAAT 
CGTTTGTCGG AGACCAGCAT GGGATCCTCA GACAGCGGAC TGTCAAAGTC AACCATTCTG 
CCCAGGCCGC CGACCAACTC CATCAAGTCC ACGCTGGCCA GCCTGAGTTT CAACACGAGG 
CCCAGCTTGG GACACTCGCC AAGCACCAAG AGCAGCAAGA ACAAGGACAA GACACCAGCC 
AAGCGGCGGT CTATGAAGAA GGATCGTGAG GCGCTTAGCT TTCCCAGCAG TCAATGGTAT 
ACGCCGCCAT TGACCACCAT TACCTCGACG CCCGAGAAGG AGATCAACAC GTCGATAGGC 
TCGCTGGCCA GCGCTTCCAA CAGCTCGTTG AGTGGCCCGA AAACACCAGA TCCTCATGTG 
CTGACCGAGG AGCTCACACC AAAGCGGCCT CTGCGCTCCG AAAAGGAGAA GGAACGTCGC 
CTCTCTAGGC CTGCCAGCAT TGCCACGCCC ACGGCCAGCA TCAAGAACTT CCCGAACGAC 
ACACGCTCCC TATCCGAGAG CAGTAATCGG AATTCAGTTG AAACCACAGA TTCCACGTCG 
GAGGAGGACA TTAGACCGCC GCCATTGCCG GCCAAGACAC GCGACTCGAC CGATTTCACA 
AGCCTGTCAC ACAACCTGGA CTGGACACCG AATAGCTATG CGATGCTCAG CACTCAGACC 
AGCTTCACCA GCAACAGCAG CAGCACGAGC ACGACAATGA CAAAGACCAG TATTACAAAC 
AGCACGTACG AGTATCTGGA GGCCACGAAC TTTAGCCTGG TGGGTGCCGG CGCCGCCTCA 
CCAGATGCCA GTAAGCCGCG TCCCCCAACG CCGCCGCCGA AGCCATCGCG GCACAGCAAA 
CACATTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003736549.1
CDS	468..6479
Translation	MTTVWSDCNAKQAFGIAKCNFDQERKPHRLNLDVGDAVIILKETTHWYYGYRQKAKETRGIFPKSYVHLCEYSIVNGEFCIQRTDIVEEITKVLLEWGAIAKSYYLATNPSFQKIRRKMNELNNNRAALISGNLPLDEVRKVKLLATAQIDTGNKILSLDMVVRDESGDILDTNAICTTELYEHHVHAVSRIEKANRLSTERGTTRSLNKYSHNILLHVNAFVCKFQEDADLLFTLFDGETHKPISENYVVKWSRTGIARDIEQIDNNRVLFTDLSRSDLNIAKMYLVCYAIRIGAMEVKDSADSKRTSMSIANSVLTAASRKHSQLSVSSNGSAGSTGEYVLRRPFGVACKDLTPIISKSEDYRGNLDLPFIMCEKDTLDGTLRKLIANKDIGKIDSKMAVTIEVLRGDIKQIKEDFPRLVHSNVPVARKMGFPEVILPGDVRNDLYLTICSGEFAKTAKNVEVSVCVANEQGSLMPGVISMGAGHPPIDEYKSVVYYHEDKPKWQETFKIHVPIEDFKQCHLRFTLKHRSSNEQRDRQDKSFVLAYVRLMQANGTTIPQGQHVLAVYKIDHKKYDKSDANCYMELPSTLAELQGTRPSNSAMSLLPKDQLTIGVNLCSTKLTQSVSLLGLLNWSAHKETLEQSLNALCSVPGEEVVKFLQDILDALFNILVENDHPEKYDQLVFMSIIHLIETVSELKYQHFLTVLDVYINESFSFTLAYTKLMDVLQKNISDAIAPREKSADAEESAEVRRLYKTTRYLHYVMKFVIRSRVLYAEMNCNTDYVDFATRLQELLRMFIDMIGCPSNLLKSEGALLKNLHIIATDLMQVFDQVRLSVSIVEILEKFPPRRLTQSKMGCIKDFVETKLFSSPKCRAILLPVFCKHIKDHLESKEEGDTKTDIWQQEKNLSKAAKVLGQSKSQLHTRETTANKKIAECINIMNNILKLLYRTDVGATHNDIRDIMIILLRTVMKAAHSLDRDTGLVGKFFAIMLGILQRMDAQHYEYFVKDLHQRGELKDFVIEILLVFEELVSPHQKAVFPRDWMDMIMHQNTVILGALKHLSVVITDYFLCPFEKQIWSNFFQCSIAFLVQSPLQLNDFNDNKRQIVFARYRDIRKDTAMEIRKMWFQLGQHKPKFVPQLVEPILEMSMIPETELRRETIPIFFDMMQCEYYSSRLELESYGDTKYNNAHHKGNFTEFKTAMIEKLDILIGAGKGDAEYKQMFEEIMLERCAAHNTLNVDGTSFVQMVTRLMDKLLEYRFIIQDESKENRMACTFSLLQFYSEVDLKEMYIRYVYKLRDLHMEFDNYTEAAFTLKLHTELLRWTDMELSPQLRSYRHSLCRTHRDLKEALYFDILEYFDKGKQWECAIDMCKVLARQYEEEIYDYIKLSELLKRMAQFYEKILKELRHSCEYFRVCFYGRKFPRLLQNRVYIFRGKEYERHSDFCTRMLQQHPQAELMQTLEAPGEDITQSDAQYIQVNKVEPIMDAAFSKFHDKIISNEIVKYYTANNVQKFRFSRPFRDSSGGGDDVRNLWLERTELITRFPLPGILRWFPVVESITFKISPLERAVEIMKDTNRDIRQLVILHKSDETLHINPLSMKLNGIVDPAVMGGFAKYEEAFLTDEYLEEHPDDKDLIEELKELIATQIPLLEIAILLHRQKAPDSLKAMQEHLESCFSDMQQHVETRYGRKTCDLKIERDSVVMRRTNSFLPALFDGGCNRLSETSMGSSDSGLSKSTILPRPPTNSIKSTLASLSFNTRPSLGHSPSTKSSKNKDKTPAKRRSMKKDREALSFPSSQWYTPPLTTITSTPEKEINTSIGSLASASNSSLSGPKTPDPHVLTEELTPKRPLRSEKEKERRLSRPASIATPTASIKNFPNDTRSLSESSNRNSVETTDSTSEEDIRPPPLPAKTRDSTDFTSLSHNLDWTPNSYAMLSTQTSFTSNSSSTSTTMTKTSITNSTYEYLEATNFSLVGAGAASPDASKPRPPTPPPKPSRHSKHIP

Target ORF information:

RefSeq Version	XM_003736501.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA10288), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003736501.2

ORF Insert Sequence:

ATGACTACTG TGTGGAGCGA TTGCAATGCT AAGCAAGCAT TCGGCATTGC CAAGTGCAAT 
TTCGATCAGG AGAGGAAGCC GCATCGCTTG AACCTGGACG TGGGCGATGC AGTCATAATA 
CTCAAGGAGA CCACCCACTG GTACTACGGC TACAGGCAAA AAGCCAAGGA AACGCGCGGC 
ATATTTCCCA AGAGCTATGT GCATCTGTGC GAGTACAGCA TTGTCAATGG GGAGTTCTGC 
ATCCAGCGCA CAGACATTGT CGAGGAGATC ACCAAAGTGC TGCTGGAGTG GGGCGCCATA 
GCCAAGAGTT ATTATTTGGC CACAAATCCG AGCTTTCAAA AGATACGCCG CAAGATGAAC 
GAACTGAACA ACAATCGAGC GGCCTTGATC TCTGGCAACC TGCCTCTGGA TGAGGTGCGG 
AAAGTGAAGC TCTTGGCCAC GGCCCAGATC GATACAGGGA ACAAGATCCT CAGCCTGGAC 
ATGGTGGTGC GCGACGAGAG CGGGGACATA CTGGACACGA ACGCCATTTG CACCACAGAG 
CTGTACGAGC ACCATGTCCA CGCCGTCTCC CGCATAGAGA AGGCGAATCG TCTCTCAACG 
GAGCGTGGAA CCACCCGCAG CCTCAACAAA TACTCGCACA ACATCCTGCT GCATGTGAAT 
GCATTCGTGT GCAAGTTCCA AGAGGACGCG GACCTGCTCT TCACACTCTT CGATGGAGAG 
ACGCACAAAC CCATCAGCGA AAACTATGTC GTGAAGTGGT CGCGCACGGG CATTGCCCGG 
GACATCGAAC AGATCGACAA CAATCGGGTG CTCTTCACGG ATCTCAGCAG AAGCGACCTG 
AACATTGCCA AAATGTATCT GGTGTGCTAT GCCATACGCA TCGGGGCGAT GGAGGTCAAG 
GACTCCGCCG ACTCGAAGCG CACCAGCATG AGCATAGCCA ACAGTGTCCT CACAGCCGCC 
AGTCGGAAGC ACTCGCAGCT GTCTGTCAGC TCCAATGGAT CTGCGGGCAG CACCGGCGAG 
TACGTACTCC GACGGCCCTT TGGCGTCGCC TGCAAGGACC TGACGCCCAT CATTAGCAAA 
TCGGAGGACT ATCGGGGCAA CCTGGACCTG CCGTTCATCA TGTGCGAGAA GGACACGCTG 
GACGGGACGC TGCGGAAGCT GATTGCCAAC AAGGACATCG GGAAGATCGA CTCCAAGATG 
GCCGTGACCA TTGAGGTGCT GCGCGGCGAC ATCAAGCAGA TCAAAGAGGA CTTCCCCCGG 
CTGGTGCACA GCAACGTCCC AGTGGCCCGC AAGATGGGCT TCCCCGAGGT CATCCTCCCA 
GGGGATGTGC GCAACGATCT GTATCTGACG ATCTGCAGCG GAGAGTTCGC CAAGACGGCC 
AAGAACGTGG AGGTGTCCGT GTGCGTGGCC AACGAGCAGG GCAGCCTCAT GCCGGGAGTC 
ATCAGCATGG GCGCCGGCCA TCCGCCCATC GACGAGTACA AGTCGGTGGT GTACTACCAC 
GAGGACAAGC CGAAGTGGCA GGAGACCTTC AAGATTCACG TGCCCATCGA GGACTTCAAG 
CAGTGCCATT TGCGGTTCAC TCTGAAGCAC CGCAGCAGCA ACGAGCAGCG CGACAGGCAG 
GACAAGTCCT TCGTTCTGGC CTATGTGCGT CTCATGCAGG CCAACGGCAC GACCATTCCG 
CAGGGCCAGC ATGTTCTGGC CGTCTACAAG ATCGATCACA AGAAGTACGA CAAGTCGGAT 
GCCAATTGCT ACATGGAACT GCCGTCGACC CTGGCCGAGC TGCAGGGCAC CAGACCCTCC 
AACAGCGCCA TGTCGCTCCT GCCTAAGGAT CAGCTGACAA TTGGGGTGAA TCTATGCAGC 
ACAAAGCTCA CGCAGAGCGT GAGCCTTTTG GGTCTTCTGA ACTGGTCTGC GCACAAGGAA 
ACCCTCGAGC AGTCGCTCAA TGCCCTGTGC TCAGTGCCCG GCGAGGAGGT GGTCAAGTTT 
CTGCAGGACA TACTCGATGC CTTGTTCAAT ATTCTGGTGG AGAACGATCA TCCGGAGAAG 
TACGATCAGC TGGTGTTCAT GAGCATCATT CATCTGATCG AAACGGTGTC CGAGCTGAAG 
TACCAGCACT TTCTCACCGT CCTGGATGTC TACATCAACG AGAGCTTCTC CTTTACGCTG 
GCCTACACCA AACTTATGGA TGTGCTGCAG AAGAACATTA GCGATGCCAT TGCCCCCCGA 
GAGAAATCCG CGGATGCCGA AGAGAGTGCA GAGGTGCGGC GCCTGTACAA GACCACGCGC 
TACCTGCACT ACGTGATGAA GTTTGTGATC AGGTCGCGGG TGCTCTATGC GGAGATGAAC 
TGCAACACGG ACTATGTGGA CTTTGCCACG CGCCTGCAGG AGCTGTTGCG CATGTTCATC 
GACATGATTG GATGCCCGAG CAATCTGCTG AAGTCCGAGG GAGCCCTGCT CAAGAATCTG 
CACATCATAG CCACCGATCT GATGCAGGTC TTTGACCAAG TGCGATTGAG CGTTTCCATT 
GTGGAGATCT TGGAGAAGTT TCCGCCGCGT CGCTTGACGC AGTCCAAGAT GGGCTGCATC 
AAGGATTTCG TGGAGACGAA GCTCTTCAGT TCGCCCAAGT GTCGGGCCAT CCTGCTGCCG 
GTGTTCTGCA AGCACATCAA GGATCATCTC GAGAGCAAAG AGGAGGGAGA CACGAAAACC 
GATATCTGGC AGCAAGAAAA GAATCTTTCG AAAGCCGCCA AAGTGCTAGG ACAGTCGAAA 
TCCCAACTGC ACACGCGTGA AACGACTGCC AACAAAAAGA TTGCCGAGTG CATCAACATC 
ATGAACAATA TACTGAAGCT GCTGTACCGC ACGGATGTGG GAGCCACCCA CAATGACATT 
CGGGACATCA TGATCATTCT GCTGCGCACC GTGATGAAGG CGGCCCACTC GCTGGACCGG 
GACACGGGGC TGGTGGGAAA GTTCTTTGCC ATTATGCTGG GCATACTGCA GCGCATGGAC 
GCCCAGCACT ATGAGTACTT TGTCAAGGAT CTGCATCAGA GGGGGGAGCT CAAGGACTTT 
GTAATCGAAA TCCTGCTGGT GTTCGAGGAG CTGGTGTCGC CGCACCAGAA GGCAGTGTTT 
CCCAGGGACT GGATGGACAT GATCATGCAC CAGAACACGG TCATCCTGGG CGCCCTAAAG 
CACCTGAGCG TTGTCATAAC CGACTACTTC CTGTGTCCCT TCGAGAAGCA GATCTGGTCA 
AACTTCTTCC AGTGCTCCAT CGCCTTCCTA GTGCAGTCGC CGCTCCAGCT GAACGACTTC 
AACGACAACA AGCGGCAGAT TGTGTTCGCC CGCTATCGCG ACATTCGCAA GGACACGGCC 
ATGGAGATAC GCAAGATGTG GTTCCAGCTG GGCCAGCACA AGCCCAAGTT TGTGCCGCAG 
CTGGTCGAAC CGATTCTGGA GATGAGCATG ATACCGGAGA CGGAGCTGCG GCGCGAGACG 
ATCCCCATCT TCTTCGACAT GATGCAGTGC GAGTACTACA GCTCGCGGCT GGAGCTGGAG 
AGCTATGGCG ACACCAAATA CAACAATGCC CACCACAAGG GAAACTTTAC GGAGTTCAAG 
ACGGCCATGA TCGAGAAGCT GGACATCCTC ATTGGAGCTG GCAAGGGCGA TGCCGAGTAC 
AAGCAGATGT TCGAGGAGAT CATGCTGGAG CGCTGTGCCG CCCACAACAC TTTGAATGTG 
GATGGGACCA GCTTTGTGCA GATGGTAACG CGTCTGATGG ACAAGCTGCT GGAGTATCGC 
TTCATCATCC AGGACGAGAG CAAGGAGAAC CGCATGGCCT GCACCTTCTC GCTGCTGCAG 
TTCTACTCGG AGGTGGACCT CAAGGAGATG TACATCCGCT ACGTCTACAA GCTGCGCGAC 
CTGCACATGG AGTTCGACAA CTACACGGAG GCCGCCTTCA CGCTGAAGCT GCACACCGAA 
CTGCTGCGCT GGACCGACAT GGAGCTGTCG CCGCAGTTGC GCAGCTATCG GCACAGCCTG 
TGCCGCACCC ACAGGGACCT GAAGGAGGCC CTGTACTTTG ATATCCTGGA GTATTTTGAC 
AAGGGCAAGC AGTGGGAGTG CGCCATCGAC ATGTGCAAGG TGCTGGCCCG CCAATACGAG 
GAGGAGATCT ACGACTACAT CAAACTGTCG GAGCTGCTGA AGCGCATGGC GCAGTTTTAC 
GAAAAGATCC TCAAGGAGCT GCGCCACAGC TGCGAGTACT TCCGCGTGTG CTTCTATGGG 
CGCAAGTTCC CGCGCCTCCT CCAGAACCGG GTGTACATCT TCCGGGGCAA GGAGTACGAG 
CGGCACAGCG ACTTCTGCAC TCGCATGCTG CAGCAGCATC CGCAGGCGGA GCTCATGCAA 
ACACTGGAGG CGCCAGGGGA GGACATCACA CAAAGCGATG CCCAGTACAT CCAAGTCAAC 
AAGGTGGAGC CCATTATGGA CGCGGCCTTT AGCAAGTTCC ACGACAAGAT CATTTCCAAC 
GAGATCGTCA AGTACTACAC CGCCAACAAT GTGCAGAAGT TCCGGTTCTC GCGACCCTTC 
CGCGACAGCA GCGGCGGCGG CGATGATGTG CGGAACCTTT GGCTCGAGCG CACGGAGCTG 
ATCACGCGCT TCCCCCTGCC CGGCATCCTG CGCTGGTTCC CCGTCGTCGA GTCCATTACG 
TTCAAGATTT CCCCTCTAGA GCGTGCAGTG GAGATCATGA AGGACACGAA CCGGGACATT 
AGGCAGCTGG TAATCCTGCA CAAAAGCGAC GAGACGCTGC ACATCAATCC GCTGAGCATG 
AAGCTCAATG GGATCGTGGA TCCGGCCGTG ATGGGAGGCT TCGCCAAGTA CGAGGAGGCC 
TTCCTGACCG ACGAGTATCT GGAGGAGCAT CCCGATGACA AGGATCTGAT CGAGGAGCTC 
AAAGAACTGA TTGCCACGCA GATACCGCTG CTGGAGATAG CCATTCTGCT GCACCGCCAG 
AAGGCGCCCG ATAGCCTAAA GGCTATGCAG GAGCACCTGG AGAGCTGCTT CTCGGACATG 
CAGCAGCACG TGGAGACGCG CTACGGCCGG AAGACCTGTG ACCTGAAGAT TGAACGCGAT 
TCTGTGGTGA TGCGACGCAC CAACTCCTTT CTGCCGGCCC TCTTCGATGG CGGCTGCAAT 
CGTTTGTCGG AGACCAGCAT GGGATCCTCA GACAGCGGAC TGTCAAAGTC AACCATTCTG 
CCCAGGCCGC CGACCAACTC CATCAAGTCC ACGCTGGCCA GCCTGAGTTT CAACACGAGG 
CCCAGCTTGG GACACTCGCC AAGCACCAAG AGCAGCAAGA ACAAGGACAA GACACCAGCC 
AAGCGGCGGT CTATGAAGAA GGATCGTGAG GCGCTTAGCT TTCCCAGCAG TCAATGGTAT 
ACGCCGCCAT TGACCACCAT TACCTCGACG CCCGAGAAGG AGATCAACAC GTCGATAGGC 
TCGCTGGCCA GCGCTTCCAA CAGCTCGTTG AGTGGCCCGA AAACACCAGA TCCTCATGTG 
CTGACCGAGG AGCTCACACC AAAGCGGCCT CTGCGCTCCG AAAAGGAGAA GGAACGTCGC 
CTCTCTAGGC CTGCCAGCAT TGCCACGCCC ACGGCCAGCA TCAAGAACTT CCCGAACGAC 
ACACGCTCCC TATCCGAGAG CAGTAATCGG AATTCAGTTG AAACCACAGA TTCCACGTCG 
GAGGAGGACA TTAGACCGCC GCCATTGCCG GCCAAGACAC GCGACTCGAC CGATTTCACA 
AGCCTGTCAC ACAACCTGGA CTGGACACCG AATAGCTATG CGATGCTCAG CACTCAGACC 
AGCTTCACCA GCAACAGCAG CAGCACGAGC ACGACAATGA CAAAGACCAG TATTACAAAC 
AGCACGTACG AGTATCTGGA GGCCACGAAC TTTAGCCTGG TGGGTGCCGG CGCCGCCTCA 
CCAGATGCCA GTAAGCCGCG TCCCCCAACG CCGCCGCCGA AGCCATCGCG GCACAGCAAA 
CACATTCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA10288 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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