mdlc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mdlc
Entrez Gene ID	42397
Full Name	midlife crisis
Synonyms	CG4973,Dmel\CG4973
General protein information	Preferred Names midlife crisis Names CG4973 gene product from transcript CG4973-RA CG4973-PA mdlc-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92C4-92C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142608.4	NP_650865.1	midlife crisis

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007068	XP_308695.4
Danio rerio (zebrafish)	rnf113a	NP_001004536.1
Caenorhabditis elegans (roundworm)	rnf-113	NP_499375.1
Arabidopsis thaliana (thale cress)	AT5G06420	NP_196260.1
Xenopus tropicalis (tropical clawed frog)	LOC548788	NP_001016034.1
Mus musculus (house mouse)	Rnf113a1	NP_705723.1
Gallus gallus (chicken)	RNF113A	NP_001004396.1
Arabidopsis thaliana (thale cress)	AT1G01350	NP_171642.2
Oryza sativa (rice)	Os02g0301000	NP_001046620.1
Bos taurus (cattle)	LOC518027	XP_005226829.1
Rattus norvegicus (Norway rat)	Rnf113a1	NP_001014791.1
Drosophila melanogaster (fruit fly)	CG4973	NP_650865.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mdlc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mdlc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08570	NM_142608.4 Latest version!	Drosophila melanogaster midlife crisis (mdlc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mdlc ( NM_142608.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650865.1 Drosophila melanogaster midlife crisis (mdlc), mRNA.

Price & Availability↑

CloneID	OFa08570
Clone ID Related Accession (Same CDS sequence)	NM_142608.4
Accession Version	NM_142608.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	midlife crisis
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142608.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAG AAGCGGCTAG CACCTCGTCC TTTGGCTTCA AGAAGCGGAA CCTCAAGAAG 
GGAGCTGGAA TTCGTCGCAA AAAGGAAACC AGCAGTGATG AATCTGCAAA AAGCAGCGAT 
GAAGAGACCA AAGGCAAAGC CAGTGCTCTG GTGCGAGCCG AGAACCGCCG AAAGCGCACG 
AATCCCAACT TCCAGAGCAC CAAGACAATG AGCAAAGCAA AGAGACAGGC GGGAGGAGTT 
GGTGATGATG GCTCATCCTC AGCCTCCGAC GATGATAAAC TGGGCATTGC GTACAAATCC 
AAACGCGAGG CGCTGCCCAG TGGACCCCAA GATCAGGGAG CTACATCGAT TAACGAAATG 
GACACAGAAT TGGATCGCGA TGCGCAGGCA ATTCATGCGC GAGCTCTGAA AATCAACGAA 
GAGCTGGAGG GCAAGGCGGA CGATAAGATC TATCGCGGAA TTAACAACTA TGCGCAGTAC 
TACAAGAAGC AGGATACAGC GGCAGGGAAT GCCAGCTCCG GAATGGTACG AAGTGGCCCA 
ATTAGGGCTC CTGCCCATCT TAGGGCCACT GTCAGGTGGG ATTATCAGCC GGATATATGC 
AAGGATTACA AGGAAACAGG ATATTGCGGC TTTGGCGACA GCTGTAAGTT TCTGCACGAT 
CGCAGTGATT ACAAAGCGGG CTGGCAATTG GAAATGGATC ACGAAAATCA GCGAACGGGT 
GATGTAGACT CCGATGGCGA TGATACCAAG TATGAGATTC ACTCCGATGA AGAAACACTG 
CCTTTCAAGT GCCACATCTG CCGACAGAGT TTTGTCAATC CCGTGGTAAC AAAGTGCAAG 
CACTATTTCT GTGAAAAGTG CGCCTTGGCC CAGTACAAAA AGTCACAGCG CTGCATCATC 
TGTTCCCAGC AAACAAATGG TATTTTCAAT CCCGCCAAAG AGCTGATAGC TCGCCTCAAG 
ACAAACCCAA TGGAAAACTC AGATAGTGAT GAGGAGGAGG AATTTGGGAA AAAGGAGGAT 
AAAGCGGAAG CAGGGGAGGA AGCTCAGGAG ATTTCTTCGG ATGATAGTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650865.1
CDS	107..1180
Translation	MSEEAASTSSFGFKKRNLKKGAGIRRKKETSSDESAKSSDEETKGKASALVRAENRRKRTNPNFQSTKTMSKAKRQAGGVGDDGSSSASDDDKLGIAYKSKREALPSGPQDQGATSINEMDTELDRDAQAIHARALKINEELEGKADDKIYRGINNYAQYYKKQDTAAGNASSGMVRSGPIRAPAHLRATVRWDYQPDICKDYKETGYCGFGDSCKFLHDRSDYKAGWQLEMDHENQRTGDVDSDGDDTKYEIHSDEETLPFKCHICRQSFVNPVVTKCKHYFCEKCALAQYKKSQRCIICSQQTNGIFNPAKELIARLKTNPMENSDSDEEEEFGKKEDKAEAGEEAQEISSDDSD

Target ORF information:

RefSeq Version	NM_142608.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster midlife crisis (mdlc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142608.4

ORF Insert Sequence:

ATGTCGGAAG AAGCGGCTAG CACCTCGTCC TTTGGCTTCA AGAAGCGGAA CCTCAAGAAG 
GGAGCTGGAA TTCGTCGCAA AAAGGAAACC AGCAGTGATG AATCTGCAAA AAGCAGCGAT 
GAAGAGACCA AAGGCAAAGC CAGTGCTCTG GTGCGAGCCG AGAACCGCCG AAAGCGCACG 
AATCCCAACT TCCAGAGCAC CAAGACAATG AGCAAAGCAA AGAGACAGGC GGGAGGAGTT 
GGTGATGATG GCTCATCCTC AGCCTCCGAC GATGATAAAC TGGGCATTGC GTACAAATCC 
AAACGCGAGG CGCTGCCCAG TGGACCCCAA GATCAGGGAG CTACATCGAT TAACGAAATG 
GACACAGAAT TGGATCGCGA TGCGCAGGCA ATTCATGCGC GAGCTCTGAA AATCAACGAA 
GAGCTGGAGG GCAAGGCGGA CGATAAGATC TATCGCGGAA TTAACAACTA TGCGCAGTAC 
TACAAGAAGC AGGATACAGC GGCAGGGAAT GCCAGCTCCG GAATGGTACG AAGTGGCCCA 
ATTAGGGCTC CTGCCCATCT TAGGGCCACT GTCAGGTGGG ATTATCAGCC GGATATATGC 
AAGGATTACA AGGAAACAGG ATATTGCGGC TTTGGCGACA GCTGTAAGTT TCTGCACGAT 
CGCAGTGATT ACAAAGCGGG CTGGCAATTG GAAATGGATC ACGAAAATCA GCGAACGGGT 
GATGTAGACT CCGATGGCGA TGATACCAAG TATGAGATTC ACTCCGATGA AGAAACACTG 
CCTTTCAAGT GCCACATCTG CCGACAGAGT TTTGTCAATC CCGTGGTAAC AAAGTGCAAG 
CACTATTTCT GTGAAAAGTG CGCCTTGGCC CAGTACAAAA AGTCACAGCG CTGCATCATC 
TGTTCCCAGC AAACAAATGG TATTTTCAAT CCCGCCAAAG AGCTGATAGC TCGCCTCAAG 
ACAAACCCAA TGGAAAACTC AGATAGTGAT GAGGAGGAGG AATTTGGGAA AAAGGAGGAT 
AAAGCGGAAG CAGGGGAGGA AGCTCAGGAG ATTTCTTCGG ATGATAGTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mdlc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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