nerfin-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nerfin-2
Entrez Gene ID	41235
Full Name	nervous fingers 2
Synonyms	CG12809,Dmel\CG12809,Nerfin
General protein information	Preferred Names nervous fingers 2 Names CG12809 gene product from transcript CG12809-RA CG12809-PA CG12809-PB nerfin-2-PA nerfin-2-PB nervous fingers
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85F9-85F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275517.1	NP_001262446.1	nervous fingers 2, isoform B
NM_079576.3	NP_524300.1	nervous fingers 2, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nerfin-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nerfin-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04429	NM_079576.3 Latest version!	Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa08244	NM_001275517.1 Latest version!	Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nerfin-2 ( NM_079576.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524300.1 Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04429
Clone ID Related Accession (Same CDS sequence)	NM_079576.3
Accession Version	NM_079576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	nervous fingers 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079576.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA TGAATCCGCC GACGCACCCG CACCCGCACC ATCACCCACA TCCTCATCCA 
CACCCACAAC CGCAGTCCCA TCTCCAGTCG GTGCAGCATC TTGGCCAGCA TCAGCCACTG 
CCCGCCCTGC TCCAGGGCCA CCCAGACACC ACTCACTTCG TCGAGTGTCG GGCATTACCA 
GCAATCAACG AGACATTTCC GCAATTTGGC CAGCATCGTC CGGCTATTTC GCTGCTCTCC 
CCCTTGGATC TTTCATTGCG CGCCGCGGCC AGTGTAGTGC CAATTACTCC ACCCTCAACG 
CCCTCGCCGC CTCGCAAGCG CCAGAGATTA ATGTCCGAGG AGAATTACCT GTGGCGTCCG 
CACATGGCGA GTCCTGCAGC TCCAGCTCCA GTTGTTCCGT CGAGCCTGGC AATGCAGCCG 
GGCTACTTTC ATCATCATCA GCAGCAGCAA CATCAACTAC CACACCAGCA ACATCATCAG 
CCATATGCCA CACGCAGCAG CTTGGAGGGC GCTTCCTTTA ATAAAAGTCA TTTTCACGGG 
ACGAAAAATA ACTTTGAGCA AATTCCGGCG GGTCAATCGA GCGTCAGCTC GCCCATCTAC 
GTGGAAGATG AGACCATTGT GCTGACCGAG GACGAGGATG AAACAGTTGT CAGTTCCCAT 
GACTACAATG AGTACGCCAC GGACACGGAG GAAGTCAACG AGGGCGACGA TGAGACGCAG 
CAGGTACAAG TCAGCGTTAA TGGCGACGAT GATGAGGAAG AGGTGTTTGT CGATGTCCTG 
GGCAGTGACG ATGACGAGGA TGAGGCGACG GACCTGGTGG CCACAAGATT GCAGGCCCAA 
CTAATAGAGA CCACGGCCCT GAAACGCAAT GAACTTCTGT ATGAGGATGA GGAGCTGCAC 
AACCAGGCGG TAAATGGTTT GGCCAGACTC TTTGACCGGG ATTTTCCAGA GCCAGATGTG 
GAGGTTTCCG GTTTGGCGGA ACTCCAGAGC AACAGCGGGA AGACATACAC GGATCTGAGT 
GGCGTGGCAA CGGCGGAGAT TAGGACTACC AGTTCCGACA ACCTTCCACC GCCTCCTCCA 
CCTCCTCCGC CTCCAGCGAC TTCTACAATC CCTGCTACAC CTCCACTTCC GCCTCCACCC 
ATATCTCGAC CCCACAAGGC GGAACGTAGG AGGTCGAAGC TACGAAAACA CATGGCCATC 
GATGAGGAGA CCATAAGTCC AGTTTCTGGC ACCATCATTC GCAAATTGCG CGACGACGAG 
GAGCTGGTGG TGCGCAAAGG CGACATTGAT CCGGCTTTTA ACGTGGTCGA AATCACCGAG 
GAGGCCAAGG CCATCCTGGC CAGCATTGAC AACAAGATTG GAGACTACAT GTGCCAGCTC 
TGTCGCACTG TCTACGATGA TGCCTTTATG TTGGCCCAGC ACCGTTGTCC CCGCATCGTT 
CACATCGAGT ACAAGTGCTC CGAGTGCGAA AAGGTCTTTA ATTGCCCGGC CAACTTGGCC 
TCCCATCGCA GATGGCACAA GCCCAAAGCG GAGGCGGCTG GAGTTAATCC CGCCAAGAAA 
CGAGTTGTGG AAACGGGCGA TCTGGTACAG GAGGCGACTA GGAGCGGCGA TGATGCCAGC 
GATGGCATTT ATCCTTGCCA TATATGCGGA AAGACTTTTC GACGTCAAGC TTACCTAAAG 
AAACACCAGG CCTCCCATCA GATGTTGGAT AATCTCAAGA ATCTGGATTT CTTCAAGTCC 
CAGCAGCAGC AGTTGACCAT CAATGGCCAC CAATTGGCCG ATCATGTCCA GCTGCAGCAT 
ACAACTGGAC AGGCGGTCAC ATCTGCCGCT CCCTATGCAT CCTTGCCACG CCCACCGCCT 
GGCATGCCCA TGTACCCGCC GCCAAGTGGT AAGAACTATC CGGGTGGACA ACGCTTTCCG 
GGATTTCCCT TTCAGTCATT CGATCAGCGT CGATTCTACT CTCTTGGGGA ATTTTACCTA 
TCGCAGCACT TGGAACGATC CTCGGCCTTC CAATACGTGC AGGCCAATCA CCTGAGGCAA 
CTGTCCAATG TGGCCCAAAC TTTGATGCCT CCTCTGCCCG TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524300.1
CDS	59..2146
Translation	MMNMNPPTHPHPHHHPHPHPHPQPQSHLQSVQHLGQHQPLPALLQGHPDTTHFVECRALPAINETFPQFGQHRPAISLLSPLDLSLRAAASVVPITPPSTPSPPRKRQRLMSEENYLWRPHMASPAAPAPVVPSSLAMQPGYFHHHQQQQHQLPHQQHHQPYATRSSLEGASFNKSHFHGTKNNFEQIPAGQSSVSSPIYVEDETIVLTEDEDETVVSSHDYNEYATDTEEVNEGDDETQQVQVSVNGDDDEEEVFVDVLGSDDDEDEATDLVATRLQAQLIETTALKRNELLYEDEELHNQAVNGLARLFDRDFPEPDVEVSGLAELQSNSGKTYTDLSGVATAEIRTTSSDNLPPPPPPPPPPATSTIPATPPLPPPPISRPHKAERRRSKLRKHMAIDEETISPVSGTIIRKLRDDEELVVRKGDIDPAFNVVEITEEAKAILASIDNKIGDYMCQLCRTVYDDAFMLAQHRCPRIVHIEYKCSECEKVFNCPANLASHRRWHKPKAEAAGVNPAKKRVVETGDLVQEATRSGDDASDGIYPCHICGKTFRRQAYLKKHQASHQMLDNLKNLDFFKSQQQQLTINGHQLADHVQLQHTTGQAVTSAAPYASLPRPPPGMPMYPPPSGKNYPGGQRFPGFPFQSFDQRRFYSLGEFYLSQHLERSSAFQYVQANHLRQLSNVAQTLMPPLPVK

Target ORF information:

RefSeq Version	NM_079576.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079576.3

ORF Insert Sequence:

ATGATGAACA TGAATCCGCC GACGCACCCG CACCCGCACC ATCACCCACA TCCTCATCCA 
CACCCACAAC CGCAGTCCCA TCTCCAGTCG GTGCAGCATC TTGGCCAGCA TCAGCCACTG 
CCCGCCCTGC TCCAGGGCCA CCCAGACACC ACTCACTTCG TCGAGTGTCG GGCATTACCA 
GCAATCAACG AGACATTTCC GCAATTTGGC CAGCATCGTC CGGCTATTTC GCTGCTCTCC 
CCCTTGGATC TTTCATTGCG CGCCGCGGCC AGTGTAGTGC CAATTACTCC ACCCTCAACG 
CCCTCGCCGC CTCGCAAGCG CCAGAGATTA ATGTCCGAGG AGAATTACCT GTGGCGTCCG 
CACATGGCGA GTCCTGCAGC TCCAGCTCCA GTTGTTCCGT CGAGCCTGGC AATGCAGCCG 
GGCTACTTTC ATCATCATCA GCAGCAGCAA CATCAACTAC CACACCAGCA ACATCATCAG 
CCATATGCCA CACGCAGCAG CTTGGAGGGC GCTTCCTTTA ATAAAAGTCA TTTTCACGGG 
ACGAAAAATA ACTTTGAGCA AATTCCGGCG GGTCAATCGA GCGTCAGCTC GCCCATCTAC 
GTGGAAGATG AGACCATTGT GCTGACCGAG GACGAGGATG AAACAGTTGT CAGTTCCCAT 
GACTACAATG AGTACGCCAC GGACACGGAG GAAGTCAACG AGGGCGACGA TGAGACGCAG 
CAGGTACAAG TCAGCGTTAA TGGCGACGAT GATGAGGAAG AGGTGTTTGT CGATGTCCTG 
GGCAGTGACG ATGACGAGGA TGAGGCGACG GACCTGGTGG CCACAAGATT GCAGGCCCAA 
CTAATAGAGA CCACGGCCCT GAAACGCAAT GAACTTCTGT ATGAGGATGA GGAGCTGCAC 
AACCAGGCGG TAAATGGTTT GGCCAGACTC TTTGACCGGG ATTTTCCAGA GCCAGATGTG 
GAGGTTTCCG GTTTGGCGGA ACTCCAGAGC AACAGCGGGA AGACATACAC GGATCTGAGT 
GGCGTGGCAA CGGCGGAGAT TAGGACTACC AGTTCCGACA ACCTTCCACC GCCTCCTCCA 
CCTCCTCCGC CTCCAGCGAC TTCTACAATC CCTGCTACAC CTCCACTTCC GCCTCCACCC 
ATATCTCGAC CCCACAAGGC GGAACGTAGG AGGTCGAAGC TACGAAAACA CATGGCCATC 
GATGAGGAGA CCATAAGTCC AGTTTCTGGC ACCATCATTC GCAAATTGCG CGACGACGAG 
GAGCTGGTGG TGCGCAAAGG CGACATTGAT CCGGCTTTTA ACGTGGTCGA AATCACCGAG 
GAGGCCAAGG CCATCCTGGC CAGCATTGAC AACAAGATTG GAGACTACAT GTGCCAGCTC 
TGTCGCACTG TCTACGATGA TGCCTTTATG TTGGCCCAGC ACCGTTGTCC CCGCATCGTT 
CACATCGAGT ACAAGTGCTC CGAGTGCGAA AAGGTCTTTA ATTGCCCGGC CAACTTGGCC 
TCCCATCGCA GATGGCACAA GCCCAAAGCG GAGGCGGCTG GAGTTAATCC CGCCAAGAAA 
CGAGTTGTGG AAACGGGCGA TCTGGTACAG GAGGCGACTA GGAGCGGCGA TGATGCCAGC 
GATGGCATTT ATCCTTGCCA TATATGCGGA AAGACTTTTC GACGTCAAGC TTACCTAAAG 
AAACACCAGG CCTCCCATCA GATGTTGGAT AATCTCAAGA ATCTGGATTT CTTCAAGTCC 
CAGCAGCAGC AGTTGACCAT CAATGGCCAC CAATTGGCCG ATCATGTCCA GCTGCAGCAT 
ACAACTGGAC AGGCGGTCAC ATCTGCCGCT CCCTATGCAT CCTTGCCACG CCCACCGCCT 
GGCATGCCCA TGTACCCGCC GCCAAGTGGT AAGAACTATC CGGGTGGACA ACGCTTTCCG 
GGATTTCCCT TTCAGTCATT CGATCAGCGT CGATTCTACT CTCTTGGGGA ATTTTACCTA 
TCGCAGCACT TGGAACGATC CTCGGCCTTC CAATACGTGC AGGCCAATCA CCTGAGGCAA 
CTGTCCAATG TGGCCCAAAC TTTGATGCCT CCTCTGCCCG TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nerfin-2 ( NM_001275517.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262446.1 Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08244
Clone ID Related Accession (Same CDS sequence)	NM_001275517.1
Accession Version	NM_001275517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	nervous fingers 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA TGAATCCGCC GACGCACCCG CACCCGCACC ATCACCCACA TCCTCATCCA 
CACCCACAAC CGCAGTCCCA TCTCCAGTCG GTGCAGCATC TTGGCCAGCA TCAGCCACTG 
CCCGCCCTGC TCCAGGGCCA CCCAGACACC ACTCACTTCG TCGAGTGTCG GGCATTACCA 
GCAATCAACG AGACATTTCC GCAATTTGGC CAGCATCGTC CGGCTATTTC GCTGCTCTCC 
CCCTTGGATC TTTCATTGCG CGCCGCGGCC AGTGTAGTGC CAATTACTCC ACCCTCAACG 
CCCTCGCCGC CTCGCAAGCG CCAGAGATTA ATGTCCGAGG AGAATTACCT GTGGCGTCCG 
CACATGGCGA GTCCTGCAGC TCCAGCTCCA GTTGTTCCGT CGAGCCTGGC AATGCAGCCG 
GGCTACTTTC ATCATCATCA GCAGCAGCAA CATCAACTAC CACACCAGCA ACATCATCAG 
CCATATGCCA CACGCAGCAG CTTGGAGGGC GCTTCCTTTA ATAAAAGTCA TTTTCACGGG 
ACGAAAAATA ACTTTGAGCA AATTCCGGCG GGTCAATCGA GCGTCAGCTC GCCCATCTAC 
GTGGAAGATG AGACCATTGT GCTGACCGAG GACGAGGATG AAACAGTTGT CAGTTCCCAT 
GACTACAATG AGTACGCCAC GGACACGGAG GAAGTCAACG AGGGCGACGA TGAGACGCAG 
CAGGTACAAG TCAGCGTTAA TGGCGACGAT GATGAGGAAG AGGTGTTTGT CGATGTCCTG 
GGCAGTGACG ATGACGAGGA TGAGGCGACG GACCTGGTGG CCACAAGATT GCAGGCCCAA 
CTAATAGAGA CCACGGCCCT GAAACGCAAT GAACTTCTGT ATGAGGATGA GGAGCTGCAC 
AACCAGGCGG TAAATGGTTT GGCCAGACTC TTTGACCGGG ATTTTCCAGA GCCAGATGTG 
GAGGTTTCCG GTTTGGCGGA ACTCCAGAGC AACAGCGGGA AGACATACAC GGATCTGAGT 
GGCGTGGCAA CGGCGGAGAT TAGGACTACC AGTTCCGACA ACCTTCCACC GCCTCCTCCA 
CCTCCTCCGC CTCCAGCGAC TTCTACAATC CCTGCTACAC CTCCACTTCC GCCTCCACCC 
ATATCTCGAC CCCACAAGGC GGAACGTAGG AGGTCGAAGC TACGAAAACA CATGGCCATC 
GATGAGGAGA CCATAAGTCC AGTTTCTGGC ACCATCATTC GCAAATTGCG CGACGACGAG 
GAGCTGGTGG TGCGCAAAGG CGACATTGAT CCGGCTTTTA ACGTGGTCGA AATCACCGAG 
GAGGCCAAGG CCATCCTGGC CAGCATTGAC AACAAGATTG GAGACTACAT GTGCCAGCTC 
TGTCGCACTG TCTACGATGA TGCCTTTATG TTGGCCCAGC ACCGTTGTCC CCGCATCGTT 
CACATCGAGT ACAAGTGCTC CGAGTGCGAA AAGGTCTTTA ATTGCCCGGC CAACTTGGCC 
TCCCATCGCA GATGGCACAA GCCCAAAGCG GAGGCGGCTG GAGTTAATCC CGCCAAGAAA 
CGAGTTGTGG AAACGGGCGA TCTGGTACAG GAGGCGACTA GGAGCGGCGA TGATGCCAGC 
GATGGCATTT ATCCTTGCCA TATATGCGGA AAGACTTTTC GACGTCAAGC TTACCTAAAG 
AAACACCAGG CCTCCCATCA GATGTTGGAT AATCTCAAGA ATCTGGATTT CTTCAAGTCC 
CAGCAGCAGC AGTTGACCAT CAATGGCCAC CAATTGGCCG ATCATGTCCA GCTGCAGCAT 
ACAACTGGAC AGGCGGTCAC ATCTGCCGCT CCCTATGCAT CCTTGCCACG CCCACCGCCT 
GGCATGCCCA TGTACCCGCC GCCAAGTGGT AAGAACTATC CGGGTGGACA ACGCTTTCCG 
GGATTTCCCT TTCAGTCATT CGATCAGCGT CGATTCTACT CTCTTGGGGA ATTTTACCTA 
TCGCAGCACT TGGAACGATC CTCGGCCTTC CAATACGTGC AGGCCAATCA CCTGAGGCAA 
CTGTCCAATG TGGCCCAAAC TTTGATGCCT CCTCTGCCCG TCAAATGACC CAATCCGAAT 
ACGAATGTCC TGCGGCCGGT GCCACGCATT GCGGCAACAG CGACGGCAAC GGGACCTCCA 
GCGGCAGCAA CAGCAACAAG GCAAGCGGCA ACTGCCGCAG GAGTGTCCTC CGTTGGCCAC 
GCCTTTAATT GGGTGGCTCC GTCGACCGCA GGTGCAACTG CAACGGGAAG TGCAACTGCA 
ACTGCCACGG CGACGACTGC AGAGTCCGGC GGAAATGTTA TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262446.1
CDS	59..2386
Translation	MMNMNPPTHPHPHHHPHPHPHPQPQSHLQSVQHLGQHQPLPALLQGHPDTTHFVECRALPAINETFPQFGQHRPAISLLSPLDLSLRAAASVVPITPPSTPSPPRKRQRLMSEENYLWRPHMASPAAPAPVVPSSLAMQPGYFHHHQQQQHQLPHQQHHQPYATRSSLEGASFNKSHFHGTKNNFEQIPAGQSSVSSPIYVEDETIVLTEDEDETVVSSHDYNEYATDTEEVNEGDDETQQVQVSVNGDDDEEEVFVDVLGSDDDEDEATDLVATRLQAQLIETTALKRNELLYEDEELHNQAVNGLARLFDRDFPEPDVEVSGLAELQSNSGKTYTDLSGVATAEIRTTSSDNLPPPPPPPPPPATSTIPATPPLPPPPISRPHKAERRRSKLRKHMAIDEETISPVSGTIIRKLRDDEELVVRKGDIDPAFNVVEITEEAKAILASIDNKIGDYMCQLCRTVYDDAFMLAQHRCPRIVHIEYKCSECEKVFNCPANLASHRRWHKPKAEAAGVNPAKKRVVETGDLVQEATRSGDDASDGIYPCHICGKTFRRQAYLKKHQASHQMLDNLKNLDFFKSQQQQLTINGHQLADHVQLQHTTGQAVTSAAPYASLPRPPPGMPMYPPPSGKNYPGGQRFPGFPFQSFDQRRFYSLGEFYLSQHLERSSAFQYVQANHLRQLSNVAQTLMPPLPVK*PNPNTNVLRPVPRIAATATATGPPAAATATRQAATAAGVSSVGHAFNWVAPSTAGATATGSATATATATTAESGGNVIM

Target ORF information:

RefSeq Version	NM_001275517.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nervous fingers 2 (nerfin-2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275517.1

ORF Insert Sequence:

ATGATGAACA TGAATCCGCC GACGCACCCG CACCCGCACC ATCACCCACA TCCTCATCCA 
CACCCACAAC CGCAGTCCCA TCTCCAGTCG GTGCAGCATC TTGGCCAGCA TCAGCCACTG 
CCCGCCCTGC TCCAGGGCCA CCCAGACACC ACTCACTTCG TCGAGTGTCG GGCATTACCA 
GCAATCAACG AGACATTTCC GCAATTTGGC CAGCATCGTC CGGCTATTTC GCTGCTCTCC 
CCCTTGGATC TTTCATTGCG CGCCGCGGCC AGTGTAGTGC CAATTACTCC ACCCTCAACG 
CCCTCGCCGC CTCGCAAGCG CCAGAGATTA ATGTCCGAGG AGAATTACCT GTGGCGTCCG 
CACATGGCGA GTCCTGCAGC TCCAGCTCCA GTTGTTCCGT CGAGCCTGGC AATGCAGCCG 
GGCTACTTTC ATCATCATCA GCAGCAGCAA CATCAACTAC CACACCAGCA ACATCATCAG 
CCATATGCCA CACGCAGCAG CTTGGAGGGC GCTTCCTTTA ATAAAAGTCA TTTTCACGGG 
ACGAAAAATA ACTTTGAGCA AATTCCGGCG GGTCAATCGA GCGTCAGCTC GCCCATCTAC 
GTGGAAGATG AGACCATTGT GCTGACCGAG GACGAGGATG AAACAGTTGT CAGTTCCCAT 
GACTACAATG AGTACGCCAC GGACACGGAG GAAGTCAACG AGGGCGACGA TGAGACGCAG 
CAGGTACAAG TCAGCGTTAA TGGCGACGAT GATGAGGAAG AGGTGTTTGT CGATGTCCTG 
GGCAGTGACG ATGACGAGGA TGAGGCGACG GACCTGGTGG CCACAAGATT GCAGGCCCAA 
CTAATAGAGA CCACGGCCCT GAAACGCAAT GAACTTCTGT ATGAGGATGA GGAGCTGCAC 
AACCAGGCGG TAAATGGTTT GGCCAGACTC TTTGACCGGG ATTTTCCAGA GCCAGATGTG 
GAGGTTTCCG GTTTGGCGGA ACTCCAGAGC AACAGCGGGA AGACATACAC GGATCTGAGT 
GGCGTGGCAA CGGCGGAGAT TAGGACTACC AGTTCCGACA ACCTTCCACC GCCTCCTCCA 
CCTCCTCCGC CTCCAGCGAC TTCTACAATC CCTGCTACAC CTCCACTTCC GCCTCCACCC 
ATATCTCGAC CCCACAAGGC GGAACGTAGG AGGTCGAAGC TACGAAAACA CATGGCCATC 
GATGAGGAGA CCATAAGTCC AGTTTCTGGC ACCATCATTC GCAAATTGCG CGACGACGAG 
GAGCTGGTGG TGCGCAAAGG CGACATTGAT CCGGCTTTTA ACGTGGTCGA AATCACCGAG 
GAGGCCAAGG CCATCCTGGC CAGCATTGAC AACAAGATTG GAGACTACAT GTGCCAGCTC 
TGTCGCACTG TCTACGATGA TGCCTTTATG TTGGCCCAGC ACCGTTGTCC CCGCATCGTT 
CACATCGAGT ACAAGTGCTC CGAGTGCGAA AAGGTCTTTA ATTGCCCGGC CAACTTGGCC 
TCCCATCGCA GATGGCACAA GCCCAAAGCG GAGGCGGCTG GAGTTAATCC CGCCAAGAAA 
CGAGTTGTGG AAACGGGCGA TCTGGTACAG GAGGCGACTA GGAGCGGCGA TGATGCCAGC 
GATGGCATTT ATCCTTGCCA TATATGCGGA AAGACTTTTC GACGTCAAGC TTACCTAAAG 
AAACACCAGG CCTCCCATCA GATGTTGGAT AATCTCAAGA ATCTGGATTT CTTCAAGTCC 
CAGCAGCAGC AGTTGACCAT CAATGGCCAC CAATTGGCCG ATCATGTCCA GCTGCAGCAT 
ACAACTGGAC AGGCGGTCAC ATCTGCCGCT CCCTATGCAT CCTTGCCACG CCCACCGCCT 
GGCATGCCCA TGTACCCGCC GCCAAGTGGT AAGAACTATC CGGGTGGACA ACGCTTTCCG 
GGATTTCCCT TTCAGTCATT CGATCAGCGT CGATTCTACT CTCTTGGGGA ATTTTACCTA 
TCGCAGCACT TGGAACGATC CTCGGCCTTC CAATACGTGC AGGCCAATCA CCTGAGGCAA 
CTGTCCAATG TGGCCCAAAC TTTGATGCCT CCTCTGCCCG TCAAATGACC CAATCCGAAT 
ACGAATGTCC TGCGGCCGGT GCCACGCATT GCGGCAACAG CGACGGCAAC GGGACCTCCA 
GCGGCAGCAA CAGCAACAAG GCAAGCGGCA ACTGCCGCAG GAGTGTCCTC CGTTGGCCAC 
GCCTTTAATT GGGTGGCTCC GTCGACCGCA GGTGCAACTG CAACGGGAAG TGCAACTGCA 
ACTGCCACGG CGACGACTGC AGAGTCCGGC GGAAATGTTA TCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nerfin-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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