Sardh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sardh
Entrez Gene ID	37026
Full Name	Sarcosine dehydrogenase
Synonyms	CG6385,Dmel\CG6385,sardh
General protein information	Preferred Names Sarcosine dehydrogenase Names CG6385 gene product from transcript CG6385-RA CG6385-PA Sardh-PA sarcosine dehydrogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54E8-54E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_137419.3	NP_611263.1	sarcosine dehydrogenase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SARDH	XP_548398.2
Danio rerio (zebrafish)	sardh	NP_957422.1
Magnaporthe oryzae (rice blast fungus)	MGG_12421	XP_003715612.1
Mus musculus (house mouse)	Sardh	NP_619606.1
Xenopus tropicalis (tropical clawed frog)	sardh	XP_002942323.2
Gallus gallus (chicken)	SARDH	XP_004946012.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007123	XP_308638.4
Macaca mulatta (Rhesus monkey)	SARDH	XP_002799978.1
Bos taurus (cattle)	SARDH	NP_001179970.1
Drosophila melanogaster (fruit fly)	CG6385	NP_611263.1
Homo sapiens (human)	SARDH	NP_001128179.1
Pan troglodytes (chimpanzee)	SARDH	XP_003312436.1
Rattus norvegicus (Norway rat)	Sardh	NP_446116.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sardh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sardh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10386	NM_137419.3 Latest version!	Drosophila melanogaster sarcosine dehydrogenase (Sardh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sardh ( NM_137419.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611263.1 Drosophila melanogaster sarcosine dehydrogenase (Sardh), mRNA.

Price & Availability↑

CloneID	OFa10386
Clone ID Related Accession (Same CDS sequence)	NM_137419.3
Accession Version	NM_137419.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	sarcosine dehydrogenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137419.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGCC ATCGCATCCT GCAGCAAGCT TCGCGCCGTG GAATCAGCAA ACAAGTGAGG 
GAAGATGGAG GAGCTCGAAG GGAACCAAGT GGATTCCTGC CAGGCGCAGC AGATGTTGTG 
GTTATTGGCG GTGGTTCGGC GGGCTGTCAC ACACTGTATC ACCTGGCACG ACGTGGTGTC 
AAGGCCGTGC TCCTGGAACG CGCCCAACTT ACGGCCGGAA CCACCTGGCA CACGGCTGGA 
TTGCTGTGGC GCCTGCGTCC CAACGATGTG GATATTCAGC TGCTGGCTAA TTCACGTCGC 
ATGCTGCAGC AATTGGAGGA GGAAACGGAG CTGGATCCGG GATGGATTCA AAATGGTGGC 
ATCTTCATTG CTCACAATGA AACGCGACTG GACGAGTATC GCAGACTGGC CACTGTGGGC 
TCAGCTTTGG GTATTGAAAA CCAAGTGCTG AGTCCGGAGG ACACCCAAAA GTTATTTCCC 
CTCCTCGATC CGTCGGCATT TGTGGGCGCT CTCTATTCTC CGGGTGATGG TGTCATGGAT 
CCGGCGATGC TGTGTGCGGC CCTCAAAAAG GCAGCCACTA ATCTCGGAGC ACAGGTAATC 
GAGAATTGCG GAGTGGATGA TCTACTGCTG GAGCAGACTG CGAGGGGTAA GAAGGTGGTG 
GGTGTTTCCA CGCCTTTCGG GGACATCAAA GCTGAGAAGG TAGTGAATGC TACAGGAGTG 
TGGGGTCGTG ATTTGGTGGC CCGGCACGGC ACCCATCTTC CCCTTGTGCC CATGAAGCAT 
GCTTATATTG TTTCTGAATC CATTCCGGGT GTTAGAGGTC TACCCAATAT CAGAGATCAC 
GATTACTCCA CCTACTTCCG CATCCAAGGG GATGCCATCT GCATGGGTGG CTATGAACCG 
AATCCCATTC TGCTGGAGCC CGTGCCCAAG GACTTCCATT TCGGACTCTA CGAACTGGAT 
TGGTCGGTGT TCGAGACCCA TGTCGAGGGA GCCCAGAAAT TGTGTCCAAG CTATGCGAAA 
TATGGCGTGA AGAGCACGGT TTGTGGCCCG GAATCCTTTA CACCCGACCA CAAACCCTTG 
ATGGGTCCGG ATCCCAACCT AGATGGGCTC TACCACAATT GTGGCTTCAA TTCAGCGGGC 
ATGATGTTTG GTGGTGGTTG CGGAGAGCAG ACCGCCTTGT GGGTCATCCA AGGTCAACCG 
GACCTGCCCA TGTTTGGCTT CGATCTGCGC AGATTCACCC AGGAGCAGGG CAAAGCCATT 
CAGTGGATTA GGGAGAAGTC GCACGAGAGC TACGTGAAGA ACTACAGCAT GGTCTTCAAG 
TATGATCAAC CACTGGCGGG TCGTGATTTC CAAAAGGATC CACTTCACGA CGAGATGATG 
AAGGCAGGCG CCGTAATGGA GGAGAAGCAG GGTTGGGAAC GGCCTGGTTT CTTCCTGCCC 
ACCGGTTCCA AGAAGGCGGT GGTTCAGCCC TATGACTGGT ACGGAAGCTA TGTCCACCAG 
AGGCACAAGG ACAGCGAGTA CGAGAGAGTT CTGGATGGGG ATCTTCACTA CAGCCGCTTC 
TCGGAGTACC ACGATCTCAT CGGTTCGGAG GCCCTGGCAT GTCGCAATAA CGCCGTGGTC 
TTTAACATGA GCTACTTCGC CAAGCTGCTT CTAGATGGTC CACAGGCCCA GGAGGCCGCT 
GACTGGCTAT TCTCCGCCAA TACCAACAGG GATCCCAGCA AAACTGTCTA CACCTGTGCT 
CTAAACGATG CCGGCGGAGT GGAGGCGGAT GTGACCATTT CCCGCCTTGC TCCCGGTTCT 
GGAGAGGTCT ACAATCCCAA GATCAATGGC CAGGGCTTTT ATATTGTGGC TGGCGGCGCT 
TCTGCCTTCT ACACTTACAG TGTTTTGCTG GCAGAGATCC GTCGAAAAGG ATTCAATGCG 
AGCCTCAAGG ATCTGACTGC CGAATTGGGT GTGATTTCTA TTCAAGGACC AAACAGCCGA 
AAGATCCTGC AGCCCCTCAT CGACTGTGAT CTGTCCGATG AGCACGTAGC TCCCAACAGC 
ACTCGATTAG CCAAATTCGG TGATGTGGGT CTCCGTTTGC TGCGGGTCAG CTTTGTGGGA 
GAACTGGGCT ACGAGCTTCA TGTGCCCAAG AAGGATTGTG CTGCCGTCTA TCGGAGTCTG 
ATGAAAGCGG GAGCTGGAGA GGATTTGCGC AATGCTGGCT ACCGTTCCCT TTACTCCCTC 
AGCAGCGAAA AAGGCTATCA TCTATGGAGC TTCGATCTGC GACCGGATGA CACGCCCCTG 
GAAGCTGGCT TGGGATTCAC ATGTCGCAAA ACGGGTGCAG ATTACCGTGG CAAGGCGGCC 
ATCGAAAATC AGCGAGCTGA GGGCCTAAAG AAGCGACTGG TCTACTTGAC GCTCCGGGAT 
CAGGTGCCCA TCTGGGGTTT GGAGGGAGTC TACCGAAATG GCGAGCCCGT GGGCATACTT 
AGACGAGCTG AATATGCCTA TACCCTTGGA AAGTCCCTCG GTCAGACATA CGTGTCCCGA 
CCAGATGGCA AGATCATCGA TGCTGATTAC ATTCGGAACG GGGAGTACGA GGTGGACATC 
TTGGGCAAGA AATACCGCGC CGACTGTCAC TTGCGCAGCC CATTCGATCC TACCGGCCAA 
CGGGTTCTCG GTAACTATGC ATCCGAGTCC AAGTCCAATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611263.1
CDS	45..2729
Translation	MWRHRILQQASRRGISKQVREDGGARREPSGFLPGAADVVVIGGGSAGCHTLYHLARRGVKAVLLERAQLTAGTTWHTAGLLWRLRPNDVDIQLLANSRRMLQQLEEETELDPGWIQNGGIFIAHNETRLDEYRRLATVGSALGIENQVLSPEDTQKLFPLLDPSAFVGALYSPGDGVMDPAMLCAALKKAATNLGAQVIENCGVDDLLLEQTARGKKVVGVSTPFGDIKAEKVVNATGVWGRDLVARHGTHLPLVPMKHAYIVSESIPGVRGLPNIRDHDYSTYFRIQGDAICMGGYEPNPILLEPVPKDFHFGLYELDWSVFETHVEGAQKLCPSYAKYGVKSTVCGPESFTPDHKPLMGPDPNLDGLYHNCGFNSAGMMFGGGCGEQTALWVIQGQPDLPMFGFDLRRFTQEQGKAIQWIREKSHESYVKNYSMVFKYDQPLAGRDFQKDPLHDEMMKAGAVMEEKQGWERPGFFLPTGSKKAVVQPYDWYGSYVHQRHKDSEYERVLDGDLHYSRFSEYHDLIGSEALACRNNAVVFNMSYFAKLLLDGPQAQEAADWLFSANTNRDPSKTVYTCALNDAGGVEADVTISRLAPGSGEVYNPKINGQGFYIVAGGASAFYTYSVLLAEIRRKGFNASLKDLTAELGVISIQGPNSRKILQPLIDCDLSDEHVAPNSTRLAKFGDVGLRLLRVSFVGELGYELHVPKKDCAAVYRSLMKAGAGEDLRNAGYRSLYSLSSEKGYHLWSFDLRPDDTPLEAGLGFTCRKTGADYRGKAAIENQRAEGLKKRLVYLTLRDQVPIWGLEGVYRNGEPVGILRRAEYAYTLGKSLGQTYVSRPDGKIIDADYIRNGEYEVDILGKKYRADCHLRSPFDPTGQRVLGNYASESKSNK

Target ORF information:

RefSeq Version	NM_137419.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sarcosine dehydrogenase (Sardh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137419.3

ORF Insert Sequence:

ATGTGGCGCC ATCGCATCCT GCAGCAAGCT TCGCGCCGTG GAATCAGCAA ACAAGTGAGG 
GAAGATGGAG GAGCTCGAAG GGAACCAAGT GGATTCCTGC CAGGCGCAGC AGATGTTGTG 
GTTATTGGCG GTGGTTCGGC GGGCTGTCAC ACACTGTATC ACCTGGCACG ACGTGGTGTC 
AAGGCCGTGC TCCTGGAACG CGCCCAACTT ACGGCCGGAA CCACCTGGCA CACGGCTGGA 
TTGCTGTGGC GCCTGCGTCC CAACGATGTG GATATTCAGC TGCTGGCTAA TTCACGTCGC 
ATGCTGCAGC AATTGGAGGA GGAAACGGAG CTGGATCCGG GATGGATTCA AAATGGTGGC 
ATCTTCATTG CTCACAATGA AACGCGACTG GACGAGTATC GCAGACTGGC CACTGTGGGC 
TCAGCTTTGG GTATTGAAAA CCAAGTGCTG AGTCCGGAGG ACACCCAAAA GTTATTTCCC 
CTCCTCGATC CGTCGGCATT TGTGGGCGCT CTCTATTCTC CGGGTGATGG TGTCATGGAT 
CCGGCGATGC TGTGTGCGGC CCTCAAAAAG GCAGCCACTA ATCTCGGAGC ACAGGTAATC 
GAGAATTGCG GAGTGGATGA TCTACTGCTG GAGCAGACTG CGAGGGGTAA GAAGGTGGTG 
GGTGTTTCCA CGCCTTTCGG GGACATCAAA GCTGAGAAGG TAGTGAATGC TACAGGAGTG 
TGGGGTCGTG ATTTGGTGGC CCGGCACGGC ACCCATCTTC CCCTTGTGCC CATGAAGCAT 
GCTTATATTG TTTCTGAATC CATTCCGGGT GTTAGAGGTC TACCCAATAT CAGAGATCAC 
GATTACTCCA CCTACTTCCG CATCCAAGGG GATGCCATCT GCATGGGTGG CTATGAACCG 
AATCCCATTC TGCTGGAGCC CGTGCCCAAG GACTTCCATT TCGGACTCTA CGAACTGGAT 
TGGTCGGTGT TCGAGACCCA TGTCGAGGGA GCCCAGAAAT TGTGTCCAAG CTATGCGAAA 
TATGGCGTGA AGAGCACGGT TTGTGGCCCG GAATCCTTTA CACCCGACCA CAAACCCTTG 
ATGGGTCCGG ATCCCAACCT AGATGGGCTC TACCACAATT GTGGCTTCAA TTCAGCGGGC 
ATGATGTTTG GTGGTGGTTG CGGAGAGCAG ACCGCCTTGT GGGTCATCCA AGGTCAACCG 
GACCTGCCCA TGTTTGGCTT CGATCTGCGC AGATTCACCC AGGAGCAGGG CAAAGCCATT 
CAGTGGATTA GGGAGAAGTC GCACGAGAGC TACGTGAAGA ACTACAGCAT GGTCTTCAAG 
TATGATCAAC CACTGGCGGG TCGTGATTTC CAAAAGGATC CACTTCACGA CGAGATGATG 
AAGGCAGGCG CCGTAATGGA GGAGAAGCAG GGTTGGGAAC GGCCTGGTTT CTTCCTGCCC 
ACCGGTTCCA AGAAGGCGGT GGTTCAGCCC TATGACTGGT ACGGAAGCTA TGTCCACCAG 
AGGCACAAGG ACAGCGAGTA CGAGAGAGTT CTGGATGGGG ATCTTCACTA CAGCCGCTTC 
TCGGAGTACC ACGATCTCAT CGGTTCGGAG GCCCTGGCAT GTCGCAATAA CGCCGTGGTC 
TTTAACATGA GCTACTTCGC CAAGCTGCTT CTAGATGGTC CACAGGCCCA GGAGGCCGCT 
GACTGGCTAT TCTCCGCCAA TACCAACAGG GATCCCAGCA AAACTGTCTA CACCTGTGCT 
CTAAACGATG CCGGCGGAGT GGAGGCGGAT GTGACCATTT CCCGCCTTGC TCCCGGTTCT 
GGAGAGGTCT ACAATCCCAA GATCAATGGC CAGGGCTTTT ATATTGTGGC TGGCGGCGCT 
TCTGCCTTCT ACACTTACAG TGTTTTGCTG GCAGAGATCC GTCGAAAAGG ATTCAATGCG 
AGCCTCAAGG ATCTGACTGC CGAATTGGGT GTGATTTCTA TTCAAGGACC AAACAGCCGA 
AAGATCCTGC AGCCCCTCAT CGACTGTGAT CTGTCCGATG AGCACGTAGC TCCCAACAGC 
ACTCGATTAG CCAAATTCGG TGATGTGGGT CTCCGTTTGC TGCGGGTCAG CTTTGTGGGA 
GAACTGGGCT ACGAGCTTCA TGTGCCCAAG AAGGATTGTG CTGCCGTCTA TCGGAGTCTG 
ATGAAAGCGG GAGCTGGAGA GGATTTGCGC AATGCTGGCT ACCGTTCCCT TTACTCCCTC 
AGCAGCGAAA AAGGCTATCA TCTATGGAGC TTCGATCTGC GACCGGATGA CACGCCCCTG 
GAAGCTGGCT TGGGATTCAC ATGTCGCAAA ACGGGTGCAG ATTACCGTGG CAAGGCGGCC 
ATCGAAAATC AGCGAGCTGA GGGCCTAAAG AAGCGACTGG TCTACTTGAC GCTCCGGGAT 
CAGGTGCCCA TCTGGGGTTT GGAGGGAGTC TACCGAAATG GCGAGCCCGT GGGCATACTT 
AGACGAGCTG AATATGCCTA TACCCTTGGA AAGTCCCTCG GTCAGACATA CGTGTCCCGA 
CCAGATGGCA AGATCATCGA TGCTGATTAC ATTCGGAACG GGGAGTACGA GGTGGACATC 
TTGGGCAAGA AATACCGCGC CGACTGTCAC TTGCGCAGCC CATTCGATCC TACCGGCCAA 
CGGGTTCTCG GTAACTATGC ATCCGAGTCC AAGTCCAATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sardh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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