O-fut1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	O-fut1
Entrez Gene ID	36564
Full Name	O-fucosyltransferase 1
Synonyms	4R6,AAF58290.1,CG12366,Dmel\CG12366,O-FUT1,O-fut,OFUT1,OFut1,Ofut-1,Ofut1,l(2)SH2 2260,l(2)SH2260,ntc,nti,o-fut1
General protein information	Preferred Names O-fucosyltransferase 1 Names CG12366 gene product from transcript CG12366-RB CG12366-PA CG12366-PB O-fucosyl transferase O-fucosyltransferase O-fut1-PA O-fut1-PB neurotic
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50E1-50E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299477.1	NP_001286406.1	O-fucosyltransferase 1, isoform B
NM_137087.3	NP_610931.1	O-fucosyltransferase 1, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	POFUT1	NP_056167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011338	XP_309312.4
Pan troglodytes (chimpanzee)	POFUT1	NP_001008982.1
Danio rerio (zebrafish)	pofut1	NP_991281.2
Bos taurus (cattle)	POFUT1	NP_892031.1
Gallus gallus (chicken)	POFUT1	NP_989758.1
Drosophila melanogaster (fruit fly)	O-fut1	NP_610931.1
Caenorhabditis elegans (roundworm)	C15C7.7	NP_741744.2
Canis lupus familiaris (dog)	POFUT1	NP_001070825.1
Rattus norvegicus (Norway rat)	Pofut1	NP_001002278.1
Mus musculus (house mouse)	Pofut1	NP_536711.3
Xenopus tropicalis (tropical clawed frog)	pofut1	NP_001004774.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following O-fut1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the O-fut1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12542	NM_001299477.1 Latest version!	Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa12542	NM_137087.3 Latest version!	Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

O-fut1 ( NM_001299477.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286406.1 Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12542
Clone ID Related Accession (Same CDS sequence)	NM_001299477.1 , NM_137087.3
Accession Version	NM_001299477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	O-fucosyltransferase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGC TCAAAATGAA GCTTCGCTTC GTAAACTTAA TTTTACTATT AATAAGCTCA 
ACATGTGCCC AATTGGGGGG CGATCCCAAT GGCTACCTCA CCTACTGTCC GTGTATGGGA 
CGCTTTGGCA ACCAGGCCGA CCACTTCCTG GGATCATTGG CCTTCGCCAA GGCGCTTAAT 
CGCACCCTGA TCCTGCCGCC GTGGGTGGAG TATCGTAGGG GTGAACTGCG ATCCCGTCAG 
GTACCGTTCA ACACATATTT TGAAGTGGAG CCCCTGAAGG AATACCATCG CGTCATCACC 
ATGGCAGATT TCATGTGGCA CCTGGCCGAC GACATTTGGC CAGAATCGGA GCGAGTGTCA 
TTTTGCTACA AGGAACGATA TAGCCTTCAG CAGGAGAAGA ACGATCCAGA CAAGCCCAAT 
TGCCACGCCA AGGATGGCAA TCCTTTTGGT CCCTTTTGGG ACACTTTTCA CATAGACTTT 
GTGCGGTCAG AGTTCTATGC GCCACTTCAT TTTGATGTGC ATCATAGCAA CGAGGCTGCC 
AAGTGGCAGA CCAAATATCC TGCAGAATCA TATCCCGTAC TCGCGTTCAC CGGAGCTCCG 
GCTAGTTTTC CTGTTCAGCT AGAGAACTGC AAGCTGCAGC GCTACTTGCA GTGGAGTCAA 
CGGTATAGGG AAGCATCTAA GGATTTCATC CGAGAGCAGT TGCCTCGGGG TGCCTTTTTG 
GGCATTCATC TGCGCAACGG TATCGATTGG GTGAGAGCCT GTGAGCACGT CAAGGATAGC 
CAGCATCTGT TTGCCTCGCC GCAGTGCTTG GGCTATAAAA ATGAACGTGG TGCACTCTAC 
CCGGAGCTCT GCATGCCCTC CAAGGAGGCG ATCATCCGCC AGCTAAAGAG AACCATTAAG 
AACGTGCGCC AAACTCAGCC GGACAACGAA ATCAAATCAG TTTTCGTGGC GTCAGACTCC 
AATCACATGA TTGGTGAACT AAACACGGCC CTTAGTCGCA TGGGCATCAG TGTGCACAAG 
CTGCCGGAGG ATGATCCTTA CCTGGACTTG GCCATTCTCG GACAGTCGAA CCACTTTATC 
GGCAACTGTA TATCCTCTTA CTCGGCATTC GAAAAAAGGG AACGAGATGT GCACGGTTTT 
CCATCGTACT TCTGGGGATT CCCCAAGGAA AAGGATCGTA AGCATACAAA CGTGCACGAG 
GAGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286406.1
CDS	34..1242
Translation	MQWLKMKLRFVNLILLLISSTCAQLGGDPNGYLTYCPCMGRFGNQADHFLGSLAFAKALNRTLILPPWVEYRRGELRSRQVPFNTYFEVEPLKEYHRVITMADFMWHLADDIWPESERVSFCYKERYSLQQEKNDPDKPNCHAKDGNPFGPFWDTFHIDFVRSEFYAPLHFDVHHSNEAAKWQTKYPAESYPVLAFTGAPASFPVQLENCKLQRYLQWSQRYREASKDFIREQLPRGAFLGIHLRNGIDWVRACEHVKDSQHLFASPQCLGYKNERGALYPELCMPSKEAIIRQLKRTIKNVRQTQPDNEIKSVFVASDSNHMIGELNTALSRMGISVHKLPEDDPYLDLAILGQSNHFIGNCISSYSAFEKRERDVHGFPSYFWGFPKEKDRKHTNVHEEL

Target ORF information:

RefSeq Version	NM_001299477.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299477.1

ORF Insert Sequence:

ATGCAGTGGC TCAAAATGAA GCTTCGCTTC GTAAACTTAA TTTTACTATT AATAAGCTCA 
ACATGTGCCC AATTGGGGGG CGATCCCAAT GGCTACCTCA CCTACTGTCC GTGTATGGGA 
CGCTTTGGCA ACCAGGCCGA CCACTTCCTG GGATCATTGG CCTTCGCCAA GGCGCTTAAT 
CGCACCCTGA TCCTGCCGCC GTGGGTGGAG TATCGTAGGG GTGAACTGCG ATCCCGTCAG 
GTACCGTTCA ACACATATTT TGAAGTGGAG CCCCTGAAGG AATACCATCG CGTCATCACC 
ATGGCAGATT TCATGTGGCA CCTGGCCGAC GACATTTGGC CAGAATCGGA GCGAGTGTCA 
TTTTGCTACA AGGAACGATA TAGCCTTCAG CAGGAGAAGA ACGATCCAGA CAAGCCCAAT 
TGCCACGCCA AGGATGGCAA TCCTTTTGGT CCCTTTTGGG ACACTTTTCA CATAGACTTT 
GTGCGGTCAG AGTTCTATGC GCCACTTCAT TTTGATGTGC ATCATAGCAA CGAGGCTGCC 
AAGTGGCAGA CCAAATATCC TGCAGAATCA TATCCCGTAC TCGCGTTCAC CGGAGCTCCG 
GCTAGTTTTC CTGTTCAGCT AGAGAACTGC AAGCTGCAGC GCTACTTGCA GTGGAGTCAA 
CGGTATAGGG AAGCATCTAA GGATTTCATC CGAGAGCAGT TGCCTCGGGG TGCCTTTTTG 
GGCATTCATC TGCGCAACGG TATCGATTGG GTGAGAGCCT GTGAGCACGT CAAGGATAGC 
CAGCATCTGT TTGCCTCGCC GCAGTGCTTG GGCTATAAAA ATGAACGTGG TGCACTCTAC 
CCGGAGCTCT GCATGCCCTC CAAGGAGGCG ATCATCCGCC AGCTAAAGAG AACCATTAAG 
AACGTGCGCC AAACTCAGCC GGACAACGAA ATCAAATCAG TTTTCGTGGC GTCAGACTCC 
AATCACATGA TTGGTGAACT AAACACGGCC CTTAGTCGCA TGGGCATCAG TGTGCACAAG 
CTGCCGGAGG ATGATCCTTA CCTGGACTTG GCCATTCTCG GACAGTCGAA CCACTTTATC 
GGCAACTGTA TATCCTCTTA CTCGGCATTC GAAAAAAGGG AACGAGATGT GCACGGTTTT 
CCATCGTACT TCTGGGGATT CCCCAAGGAA AAGGATCGTA AGCATACAAA CGTGCACGAG 
GAGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

O-fut1 ( NM_137087.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610931.1 Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12542
Clone ID Related Accession (Same CDS sequence)	NM_001299477.1 , NM_137087.3
Accession Version	NM_137087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	O-fucosyltransferase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137087.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGGC TCAAAATGAA GCTTCGCTTC GTAAACTTAA TTTTACTATT AATAAGCTCA 
ACATGTGCCC AATTGGGGGG CGATCCCAAT GGCTACCTCA CCTACTGTCC GTGTATGGGA 
CGCTTTGGCA ACCAGGCCGA CCACTTCCTG GGATCATTGG CCTTCGCCAA GGCGCTTAAT 
CGCACCCTGA TCCTGCCGCC GTGGGTGGAG TATCGTAGGG GTGAACTGCG ATCCCGTCAG 
GTACCGTTCA ACACATATTT TGAAGTGGAG CCCCTGAAGG AATACCATCG CGTCATCACC 
ATGGCAGATT TCATGTGGCA CCTGGCCGAC GACATTTGGC CAGAATCGGA GCGAGTGTCA 
TTTTGCTACA AGGAACGATA TAGCCTTCAG CAGGAGAAGA ACGATCCAGA CAAGCCCAAT 
TGCCACGCCA AGGATGGCAA TCCTTTTGGT CCCTTTTGGG ACACTTTTCA CATAGACTTT 
GTGCGGTCAG AGTTCTATGC GCCACTTCAT TTTGATGTGC ATCATAGCAA CGAGGCTGCC 
AAGTGGCAGA CCAAATATCC TGCAGAATCA TATCCCGTAC TCGCGTTCAC CGGAGCTCCG 
GCTAGTTTTC CTGTTCAGCT AGAGAACTGC AAGCTGCAGC GCTACTTGCA GTGGAGTCAA 
CGGTATAGGG AAGCATCTAA GGATTTCATC CGAGAGCAGT TGCCTCGGGG TGCCTTTTTG 
GGCATTCATC TGCGCAACGG TATCGATTGG GTGAGAGCCT GTGAGCACGT CAAGGATAGC 
CAGCATCTGT TTGCCTCGCC GCAGTGCTTG GGCTATAAAA ATGAACGTGG TGCACTCTAC 
CCGGAGCTCT GCATGCCCTC CAAGGAGGCG ATCATCCGCC AGCTAAAGAG AACCATTAAG 
AACGTGCGCC AAACTCAGCC GGACAACGAA ATCAAATCAG TTTTCGTGGC GTCAGACTCC 
AATCACATGA TTGGTGAACT AAACACGGCC CTTAGTCGCA TGGGCATCAG TGTGCACAAG 
CTGCCGGAGG ATGATCCTTA CCTGGACTTG GCCATTCTCG GACAGTCGAA CCACTTTATC 
GGCAACTGTA TATCCTCTTA CTCGGCATTC GAAAAAAGGG AACGAGATGT GCACGGTTTT 
CCATCGTACT TCTGGGGATT CCCCAAGGAA AAGGATCGTA AGCATACAAA CGTGCACGAG 
GAGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610931.1
CDS	34..1242
Translation	MQWLKMKLRFVNLILLLISSTCAQLGGDPNGYLTYCPCMGRFGNQADHFLGSLAFAKALNRTLILPPWVEYRRGELRSRQVPFNTYFEVEPLKEYHRVITMADFMWHLADDIWPESERVSFCYKERYSLQQEKNDPDKPNCHAKDGNPFGPFWDTFHIDFVRSEFYAPLHFDVHHSNEAAKWQTKYPAESYPVLAFTGAPASFPVQLENCKLQRYLQWSQRYREASKDFIREQLPRGAFLGIHLRNGIDWVRACEHVKDSQHLFASPQCLGYKNERGALYPELCMPSKEAIIRQLKRTIKNVRQTQPDNEIKSVFVASDSNHMIGELNTALSRMGISVHKLPEDDPYLDLAILGQSNHFIGNCISSYSAFEKRERDVHGFPSYFWGFPKEKDRKHTNVHEEL

Target ORF information:

RefSeq Version	NM_137087.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster O-fucosyltransferase 1 (O-fut1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137087.3

ORF Insert Sequence:

ATGCAGTGGC TCAAAATGAA GCTTCGCTTC GTAAACTTAA TTTTACTATT AATAAGCTCA 
ACATGTGCCC AATTGGGGGG CGATCCCAAT GGCTACCTCA CCTACTGTCC GTGTATGGGA 
CGCTTTGGCA ACCAGGCCGA CCACTTCCTG GGATCATTGG CCTTCGCCAA GGCGCTTAAT 
CGCACCCTGA TCCTGCCGCC GTGGGTGGAG TATCGTAGGG GTGAACTGCG ATCCCGTCAG 
GTACCGTTCA ACACATATTT TGAAGTGGAG CCCCTGAAGG AATACCATCG CGTCATCACC 
ATGGCAGATT TCATGTGGCA CCTGGCCGAC GACATTTGGC CAGAATCGGA GCGAGTGTCA 
TTTTGCTACA AGGAACGATA TAGCCTTCAG CAGGAGAAGA ACGATCCAGA CAAGCCCAAT 
TGCCACGCCA AGGATGGCAA TCCTTTTGGT CCCTTTTGGG ACACTTTTCA CATAGACTTT 
GTGCGGTCAG AGTTCTATGC GCCACTTCAT TTTGATGTGC ATCATAGCAA CGAGGCTGCC 
AAGTGGCAGA CCAAATATCC TGCAGAATCA TATCCCGTAC TCGCGTTCAC CGGAGCTCCG 
GCTAGTTTTC CTGTTCAGCT AGAGAACTGC AAGCTGCAGC GCTACTTGCA GTGGAGTCAA 
CGGTATAGGG AAGCATCTAA GGATTTCATC CGAGAGCAGT TGCCTCGGGG TGCCTTTTTG 
GGCATTCATC TGCGCAACGG TATCGATTGG GTGAGAGCCT GTGAGCACGT CAAGGATAGC 
CAGCATCTGT TTGCCTCGCC GCAGTGCTTG GGCTATAAAA ATGAACGTGG TGCACTCTAC 
CCGGAGCTCT GCATGCCCTC CAAGGAGGCG ATCATCCGCC AGCTAAAGAG AACCATTAAG 
AACGTGCGCC AAACTCAGCC GGACAACGAA ATCAAATCAG TTTTCGTGGC GTCAGACTCC 
AATCACATGA TTGGTGAACT AAACACGGCC CTTAGTCGCA TGGGCATCAG TGTGCACAAG 
CTGCCGGAGG ATGATCCTTA CCTGGACTTG GCCATTCTCG GACAGTCGAA CCACTTTATC 
GGCAACTGTA TATCCTCTTA CTCGGCATTC GAAAAAAGGG AACGAGATGT GCACGGTTTT 
CCATCGTACT TCTGGGGATT CCCCAAGGAA AAGGATCGTA AGCATACAAA CGTGCACGAG 
GAGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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O-fut1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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