tgy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tgy
Entrez Gene ID	32896
Full Name	twiggy
Synonyms	CG7440,Dmel\CG7440,Tgy,dC1GalT5
General protein information	Preferred Names twiggy Names CG7440 gene product from transcript CG7440-RE CG7440-PC CG7440-PE tgy-PC tgy-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18A3-18A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_133121.5	NP_573349.4	twiggy, isoform C
NM_001298496.1	NP_001285425.1	twiggy, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tgy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tgy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02082	NM_001298496.1 Latest version!	Drosophila melanogaster twiggy (tgy), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa02082	NM_133121.5 Latest version!	Drosophila melanogaster twiggy (tgy), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tgy ( NM_133121.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573349.4 Drosophila melanogaster twiggy (tgy), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02082
Clone ID Related Accession (Same CDS sequence)	NM_133121.5 , NM_001298496.1
Accession Version	NM_133121.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	twiggy, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_133121.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGGG AATATATCAT ACTGGAGAAC TCGTCGACTT ATCCGGGCTT CCATTCCAGT 
TCCAACGGAT TGGCGAATGG CATGTACCGC TCCGCCCAGC GCATCTCCAC CGATCGCCTG 
GTCCTGCTCA TTCTGCTGGT GAGCACCACG GTGATCGGGC TGTTTGCCTA CTGGGATGTA 
ATGGTGCTCA ACGCCGGCGC ACTGCCACTG TCCAGAGGCA CTAGCTCTGT TGATTACATG 
GAGCCTGGAC TGCGCAACGA AACCCTGGCC GAGAAGATGC ATCGGGAGGT GAGAGTCCTG 
TGCTGGGTGC TCACCACACC CAAATACCAC AAGACCCGGG CCATTCACGT TTTGCGAACG 
TGGGGCAAAC GCTGCAATAA GATCTACTTC ATGACCTCTG AGCCGGATGA CGAGCTGCCC 
ACCGTTGTGC TGACCAAGCC GGACCGATAC GAGATGCTGT GGGGCAAGAC CAAAGAGGCC 
TTTGTCCACA TCCACGAGCA GATGCGCCAC GAGGCGGATT GGTTCATAAA GGCGGACGAC 
GACACCTACC TCTTTTTGGA AAATCTGCGC TACATGCTCT ACCCGTATTC ACCGGAGACA 
CCTATTTATT TTGGATTTAA TTACAAGATG GTCGGCACTC ACCAGAAGAA CGAGTCATAC 
ATGTCCGGCG GCAGTGGCTA CGTACTGAGC CGCGAGGCTC TTAGGATCTT CGCCGAGGGC 
GTCAACGACA CCACCAAGTG TCGCCAGGAG GACGATCACG CCGAGGACGT CGAGATGGGC 
AAGTGCCTGT TCAACCTGGG CGTGAAGGCG GGTGATTCCA GGGACGAGCA GCTGCGCAAC 
CGCTTCTATC CCATAGCTCC TTATGGAGCC CTTCTCTCGG GCAACGTTGG CATGGACTTT 
TGGTTGTACA AGTACGCCTA CTACAATCCG AGATCGTGCA TGGACTGCCT GTCGGAGTAC 
CCGGTGGCCT TTCACTATGT GCACAGCAAG CAGCTGTACG TGTACGACTA CTTCAACTAC 
CAGTTCCAGC TCTCCGGAAG GGCGCAGGTG GCGGAACGGC TGCCCAAGAA GATCCGGGAG 
GAGGAGCTGG TCATCCCCGA GAGCGACAAT CCGGTGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573349.4
CDS	13..1134
Translation	MAGEYIILENSSTYPGFHSSSNGLANGMYRSAQRISTDRLVLLILLVSTTVIGLFAYWDVMVLNAGALPLSRGTSSVDYMEPGLRNETLAEKMHREVRVLCWVLTTPKYHKTRAIHVLRTWGKRCNKIYFMTSEPDDELPTVVLTKPDRYEMLWGKTKEAFVHIHEQMRHEADWFIKADDDTYLFLENLRYMLYPYSPETPIYFGFNYKMVGTHQKNESYMSGGSGYVLSREALRIFAEGVNDTTKCRQEDDHAEDVEMGKCLFNLGVKAGDSRDEQLRNRFYPIAPYGALLSGNVGMDFWLYKYAYYNPRSCMDCLSEYPVAFHYVHSKQLYVYDYFNYQFQLSGRAQVAERLPKKIREEELVIPESDNPVS

Target ORF information:

RefSeq Version	NM_133121.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twiggy (tgy), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133121.5

ORF Insert Sequence:

ATGGCCGGGG AATATATCAT ACTGGAGAAC TCGTCGACTT ATCCGGGCTT CCATTCCAGT 
TCCAACGGAT TGGCGAATGG CATGTACCGC TCCGCCCAGC GCATCTCCAC CGATCGCCTG 
GTCCTGCTCA TTCTGCTGGT GAGCACCACG GTGATCGGGC TGTTTGCCTA CTGGGATGTA 
ATGGTGCTCA ACGCCGGCGC ACTGCCACTG TCCAGAGGCA CTAGCTCTGT TGATTACATG 
GAGCCTGGAC TGCGCAACGA AACCCTGGCC GAGAAGATGC ATCGGGAGGT GAGAGTCCTG 
TGCTGGGTGC TCACCACACC CAAATACCAC AAGACCCGGG CCATTCACGT TTTGCGAACG 
TGGGGCAAAC GCTGCAATAA GATCTACTTC ATGACCTCTG AGCCGGATGA CGAGCTGCCC 
ACCGTTGTGC TGACCAAGCC GGACCGATAC GAGATGCTGT GGGGCAAGAC CAAAGAGGCC 
TTTGTCCACA TCCACGAGCA GATGCGCCAC GAGGCGGATT GGTTCATAAA GGCGGACGAC 
GACACCTACC TCTTTTTGGA AAATCTGCGC TACATGCTCT ACCCGTATTC ACCGGAGACA 
CCTATTTATT TTGGATTTAA TTACAAGATG GTCGGCACTC ACCAGAAGAA CGAGTCATAC 
ATGTCCGGCG GCAGTGGCTA CGTACTGAGC CGCGAGGCTC TTAGGATCTT CGCCGAGGGC 
GTCAACGACA CCACCAAGTG TCGCCAGGAG GACGATCACG CCGAGGACGT CGAGATGGGC 
AAGTGCCTGT TCAACCTGGG CGTGAAGGCG GGTGATTCCA GGGACGAGCA GCTGCGCAAC 
CGCTTCTATC CCATAGCTCC TTATGGAGCC CTTCTCTCGG GCAACGTTGG CATGGACTTT 
TGGTTGTACA AGTACGCCTA CTACAATCCG AGATCGTGCA TGGACTGCCT GTCGGAGTAC 
CCGGTGGCCT TTCACTATGT GCACAGCAAG CAGCTGTACG TGTACGACTA CTTCAACTAC 
CAGTTCCAGC TCTCCGGAAG GGCGCAGGTG GCGGAACGGC TGCCCAAGAA GATCCGGGAG 
GAGGAGCTGG TCATCCCCGA GAGCGACAAT CCGGTGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tgy ( NM_001298496.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285425.1 Drosophila melanogaster twiggy (tgy), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa02082
Clone ID Related Accession (Same CDS sequence)	NM_133121.5 , NM_001298496.1
Accession Version	NM_001298496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	twiggy, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGGGG AATATATCAT ACTGGAGAAC TCGTCGACTT ATCCGGGCTT CCATTCCAGT 
TCCAACGGAT TGGCGAATGG CATGTACCGC TCCGCCCAGC GCATCTCCAC CGATCGCCTG 
GTCCTGCTCA TTCTGCTGGT GAGCACCACG GTGATCGGGC TGTTTGCCTA CTGGGATGTA 
ATGGTGCTCA ACGCCGGCGC ACTGCCACTG TCCAGAGGCA CTAGCTCTGT TGATTACATG 
GAGCCTGGAC TGCGCAACGA AACCCTGGCC GAGAAGATGC ATCGGGAGGT GAGAGTCCTG 
TGCTGGGTGC TCACCACACC CAAATACCAC AAGACCCGGG CCATTCACGT TTTGCGAACG 
TGGGGCAAAC GCTGCAATAA GATCTACTTC ATGACCTCTG AGCCGGATGA CGAGCTGCCC 
ACCGTTGTGC TGACCAAGCC GGACCGATAC GAGATGCTGT GGGGCAAGAC CAAAGAGGCC 
TTTGTCCACA TCCACGAGCA GATGCGCCAC GAGGCGGATT GGTTCATAAA GGCGGACGAC 
GACACCTACC TCTTTTTGGA AAATCTGCGC TACATGCTCT ACCCGTATTC ACCGGAGACA 
CCTATTTATT TTGGATTTAA TTACAAGATG GTCGGCACTC ACCAGAAGAA CGAGTCATAC 
ATGTCCGGCG GCAGTGGCTA CGTACTGAGC CGCGAGGCTC TTAGGATCTT CGCCGAGGGC 
GTCAACGACA CCACCAAGTG TCGCCAGGAG GACGATCACG CCGAGGACGT CGAGATGGGC 
AAGTGCCTGT TCAACCTGGG CGTGAAGGCG GGTGATTCCA GGGACGAGCA GCTGCGCAAC 
CGCTTCTATC CCATAGCTCC TTATGGAGCC CTTCTCTCGG GCAACGTTGG CATGGACTTT 
TGGTTGTACA AGTACGCCTA CTACAATCCG AGATCGTGCA TGGACTGCCT GTCGGAGTAC 
CCGGTGGCCT TTCACTATGT GCACAGCAAG CAGCTGTACG TGTACGACTA CTTCAACTAC 
CAGTTCCAGC TCTCCGGAAG GGCGCAGGTG GCGGAACGGC TGCCCAAGAA GATCCGGGAG 
GAGGAGCTGG TCATCCCCGA GAGCGACAAT CCGGTGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285425.1
CDS	13..1134
Translation	MAGEYIILENSSTYPGFHSSSNGLANGMYRSAQRISTDRLVLLILLVSTTVIGLFAYWDVMVLNAGALPLSRGTSSVDYMEPGLRNETLAEKMHREVRVLCWVLTTPKYHKTRAIHVLRTWGKRCNKIYFMTSEPDDELPTVVLTKPDRYEMLWGKTKEAFVHIHEQMRHEADWFIKADDDTYLFLENLRYMLYPYSPETPIYFGFNYKMVGTHQKNESYMSGGSGYVLSREALRIFAEGVNDTTKCRQEDDHAEDVEMGKCLFNLGVKAGDSRDEQLRNRFYPIAPYGALLSGNVGMDFWLYKYAYYNPRSCMDCLSEYPVAFHYVHSKQLYVYDYFNYQFQLSGRAQVAERLPKKIREEELVIPESDNPVS

Target ORF information:

RefSeq Version	NM_001298496.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twiggy (tgy), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298496.1

ORF Insert Sequence:

ATGGCCGGGG AATATATCAT ACTGGAGAAC TCGTCGACTT ATCCGGGCTT CCATTCCAGT 
TCCAACGGAT TGGCGAATGG CATGTACCGC TCCGCCCAGC GCATCTCCAC CGATCGCCTG 
GTCCTGCTCA TTCTGCTGGT GAGCACCACG GTGATCGGGC TGTTTGCCTA CTGGGATGTA 
ATGGTGCTCA ACGCCGGCGC ACTGCCACTG TCCAGAGGCA CTAGCTCTGT TGATTACATG 
GAGCCTGGAC TGCGCAACGA AACCCTGGCC GAGAAGATGC ATCGGGAGGT GAGAGTCCTG 
TGCTGGGTGC TCACCACACC CAAATACCAC AAGACCCGGG CCATTCACGT TTTGCGAACG 
TGGGGCAAAC GCTGCAATAA GATCTACTTC ATGACCTCTG AGCCGGATGA CGAGCTGCCC 
ACCGTTGTGC TGACCAAGCC GGACCGATAC GAGATGCTGT GGGGCAAGAC CAAAGAGGCC 
TTTGTCCACA TCCACGAGCA GATGCGCCAC GAGGCGGATT GGTTCATAAA GGCGGACGAC 
GACACCTACC TCTTTTTGGA AAATCTGCGC TACATGCTCT ACCCGTATTC ACCGGAGACA 
CCTATTTATT TTGGATTTAA TTACAAGATG GTCGGCACTC ACCAGAAGAA CGAGTCATAC 
ATGTCCGGCG GCAGTGGCTA CGTACTGAGC CGCGAGGCTC TTAGGATCTT CGCCGAGGGC 
GTCAACGACA CCACCAAGTG TCGCCAGGAG GACGATCACG CCGAGGACGT CGAGATGGGC 
AAGTGCCTGT TCAACCTGGG CGTGAAGGCG GGTGATTCCA GGGACGAGCA GCTGCGCAAC 
CGCTTCTATC CCATAGCTCC TTATGGAGCC CTTCTCTCGG GCAACGTTGG CATGGACTTT 
TGGTTGTACA AGTACGCCTA CTACAATCCG AGATCGTGCA TGGACTGCCT GTCGGAGTAC 
CCGGTGGCCT TTCACTATGT GCACAGCAAG CAGCTGTACG TGTACGACTA CTTCAACTAC 
CAGTTCCAGC TCTCCGGAAG GGCGCAGGTG GCGGAACGGC TGCCCAAGAA GATCCGGGAG 
GAGGAGCTGG TCATCCCCGA GAGCGACAAT CCGGTGTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tgy cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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