DDB_G0290283 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DDB_G0290283
Entrez Gene ID	8627568
Full Name	hypothetical protein
Synonyms	DDBDRAFT_0188806,DDB_0188806
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_630672.1	XP_635764.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB_G0290283 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB_G0290283 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm10922	XM_630672.1 Latest version!	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0290283) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDB_G0290283 ( XM_630672.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_635764.1 Dictyostelium discoideum AX4 hypothetical protein (DDB_G0290283) mRNA, complete cds.

Price & Availability↑

CloneID	ODm10922
Clone ID Related Accession (Same CDS sequence)	XM_630672.1
Accession Version	XM_630672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007091). COMPLETENESS: incomplete on both ends.

ATGATAAAAT TATTTTTAAA ATTATTAATT ATAATTTTAT GTTCGATATG TTTAAATAAT 
ATAATTTTTA ATAATTATTT TGTAAGTGGC CATATATTAA CAACACAAAA TGATACTACC 
AACAATAATA ATAATATAAC TTCATCATCA TCATCTTTAA ATGAATCATC AATTTCTTGT 
GGTGATTTAT CTTTAATGTC AAATCAAACA TATAAAAAAT TTAATTTAAC TTCAACATTG 
GTTCAATCAT CATCATCGTC TTCATCAGTT TCGTCAACAG TTACCAATAC CATTTTGCCA 
CAAAAACTAA ATTCGACAAC ACCATCACTT TCAAAAAATA ATGGTACAAT GATAAGTTCA 
ACAAATACAA CTAAAACATC ATCATCATTT CATTCGTCAT CATCTTCAAC AACAACAACA 
ACATCATCAT CATCATCAGC ATCTGGTTCA ACGACATCTT CGTCTGGTAC AATAGCATCT 
GGTCAATCTT CAAATTGTGT ATATTATAAT AGTAAAACAA CAATAATTAA TTCACCAGAA 
ACAGTAATTG GTGATGTAAG TAAAAAAACA TCAAATACTT TAAATGTTGC ATTCACATTA 
CAATTTAATG GATCATTATC ATTATCAAAT GGTTCAAGTA TGAATATTGG ATGTAATAAT 
AATAGTGGTG AATGTTTACG TACAACAAGT CATTTTCAAA TTGATGGAAT TGTAAATATT 
TTAGATAGTA ATAGTATTTT AGATATTAAT TCAGATTTAA ATATATCAAA AACTGGTATT 
TTACGAACAA ATATATCAGC AGCATCATCA TCATCATCAG CATCAGCATC AAATCAATTT 
GTACCAACAA TGAATGTATT AACAACATCT TCATCATCAT CTTCTTCCAC TTCAAAAGAT 
AGTAATAATA ATAATAATAA TTTACAAGTT AATTCAAAAA CAACATCAAT TAATTCAAAT 
AATTTTTATA ATAATGGTGG ATTATTTAAT ATTGATTTAC CAATATCAAT AAATACAAAC 
CTGTTTAATA TAAATAGTAA AGGTATTATT AATGTAAATT CACCATTAAC TATAAACTCA 
ACACAATCAA TTGTTAAAGG TGGTTCAATT TTAAATATTG ATAATTTATT GAACACTCAA 
GATTCAAAAG GTGGTATGTC AATTTCAGAT GGTTCAAAAG TGAATTTAAA TTCACCATTA 
TCAAATTTAA AAGGATTGTT ATTAGAATTA AGTGACTCAA ACTCAACGTT GAATGCAAAT 
AATCAATCGT TAGTCCAAAT GGATCAGTTC ATAAGTAATG GTGGTAAAAC GATTATAAAT 
AATTCACTAT TAGAGATTAA ATCTCAATTT TTGTTGGGTG ATAAATCAAC AATAAATTTA 
AATAATGAGG GTGGATTACA TGTGAATGGT AATTTCAAAT TGAATGGTAA TGGTACTCTA 
TCTATCAATT CCAATGGGTA TATAGTGAGT GATTCTGGTG ATTTTTATTT AAATGATGAT 
TCTAAAATAA TTGTAAATCA AAGTTCTATT GGAATTCGCT CAAATATTCA TTCAATTGGT 
AATTCAATAT TCCAGAATGA AAATAGTGTT TTTCAATTCA TGGGGTCATC AATTAATTTA 
TCAGATAAGT CTATATTCCT TTTAAAGAAT TGTACAGATG TAAATATGGA ATCTAAAATT 
ACAATGCTAT CTGATAATGC TCAATTCCAA TTATCATCAA ACTCATTCCT AAATACGAAT 
AATCCATTCA ATTTATTAAA TTCATCAATG TTATTAATTA GTGATAATTC AATTCATTCA 
CATAATAATA ATTTAATTCA GTTAAATCAT AATTCTCAAT TGAAAATATC AAGTAATTCA 
AAATTCTCTT TAAATGGATT AGGTTCAAAT TTATTATTAA ATGAAAATTC AATCGTAACA 
ATTGATCAAG GTTCAACTAT TAATTTTAAT AGAGGTTCTT CTTTAAAATT AAATGGTAAT 
ACAATTTTAA ATTTATCAAA TAATTCAAAT ATTTATTTTG AAAATTCAAA TTTACATTTA 
AGTGATAATT CAATAATTTC AATTGATTTT TCAAATTTTT ATTTATCAAA AACTATAATT 
AAAACTACAT CATCAACAAC ATCAAATAAT AATCATTTAT CACAAATTAA TAATCAAAGT 
TTAGTTAATT TAAATAATTC AAATTTTATA AATAATCAAA ATTTTGAAAT TAATGATGGT 
GGTAAATTTC AATTATTTGG TAATTCTTAT TTTGAAAATA ATGGTGATGT AACTTTAAAA 
TCCAATAGTT TATTTCATTC ACCTGATAAT GGTAATCATG TTATAAATAA TTATAAATCT 
TGGATGTTTT CTTCTTCTTC AGATTCTTCT TCTTCTTCCT CCTCCTCTTC ATCATCTTCT 
TCAAATTCTT CTTCAAATAC AACCAATTCA ACAGATTCAT ACTATCCTAC AACAAAACTT 
AATAAATTTA ATAATTTTGG TGATTTAATA TTAAAAGATA TGAAATTAAT ATTAAATCAA 
TTTAATACTT CAGCTAATAG TATTATTAAT TTATCAAATT CAATTTTATC AATTGATAAT 
AATAATAATA ATAATAAAAC AAATAATAGT TCAAAAACTA TATTAATTCA TAATGGTAAT 
ATTAATGGTA ATGGTATTAT TGAAAATGAT ATTAAATCAT CATTCAACTC TTCAATTGGT 
ACAAATTCAT CAATTACAAA GCTAATTGTT AATGGTTCAT TATCTCAAAA TTCAAACTCT 
CATTTCGATA TTTTAATCAA TGGTAATGAT ACTGCTGGTA TTGATTTTAC TCATATTCTT 
ATACTTGGTG AATATATAGC ATCCAATGAA GAAATTAATG ATAATGATAA TGATAGTAAT 
AATAATAATA ATTTAAAAAG ATCGATATTA TCAATTGATG GATCATTATC ATCATCAGCA 
GCAACAACCA CTAGTAATAC AAATAAAACT ATTAATGTTT ATTTAAATAA TACTAATACA 
AGTGGTAATA TTACATTTTT AAATTATTCA AATTCAAATT TAACCAATTC ATTCACATTG 
AAATTAAATT CATACAATAA AACATCAAAC TCTTATGAAA CTATTAACGA TCCCTGTAAA 
TACCAATTAA CACAAACTAA ATCATCACTT AGTTTATTAA TTTCACCAAC TAGTACTTGT 
AGAGTAACTG ATGATGGTAA AGTAATCATT ACTGATGATA ATAATAATGG TAATCTCATC 
TTTAGGAATA AAGTTATTAT CGCTGTAGTT TTATGTGTTG TCTTTAGTAC TGCAATCGTT 
GGTGTTATAA TTTGGAAAAA AAGAAAAATT AATAATTTCA TCAAAATTAA AAGAGAATAT 
TTTTTAACAA AAAGAAATAG TAGAAAGGGG ATCAAAGAGA ATCAAGAGTG TACACAAGCA 
GAAAACCTAT TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_635764.1
CDS	1..3438
Translation	MIKLFLKLLIIILCSICLNNIIFNNYFVSGHILTTQNDTTNNNNNITSSSSSLNESSISCGDLSLMSNQTYKKFNLTSTLVQSSSSSSSVSSTVTNTILPQKLNSTTPSLSKNNGTMISSTNTTKTSSSFHSSSSSTTTTTSSSSSASGSTTSSSGTIASGQSSNCVYYNSKTTIINSPETVIGDVSKKTSNTLNVAFTLQFNGSLSLSNGSSMNIGCNNNSGECLRTTSHFQIDGIVNILDSNSILDINSDLNISKTGILRTNISAASSSSSASASNQFVPTMNVLTTSSSSSSSTSKDSNNNNNNLQVNSKTTSINSNNFYNNGGLFNIDLPISINTNLFNINSKGIINVNSPLTINSTQSIVKGGSILNIDNLLNTQDSKGGMSISDGSKVNLNSPLSNLKGLLLELSDSNSTLNANNQSLVQMDQFISNGGKTIINNSLLEIKSQFLLGDKSTINLNNEGGLHVNGNFKLNGNGTLSINSNGYIVSDSGDFYLNDDSKIIVNQSSIGIRSNIHSIGNSIFQNENSVFQFMGSSINLSDKSIFLLKNCTDVNMESKITMLSDNAQFQLSSNSFLNTNNPFNLLNSSMLLISDNSIHSHNNNLIQLNHNSQLKISSNSKFSLNGLGSNLLLNENSIVTIDQGSTINFNRGSSLKLNGNTILNLSNNSNIYFENSNLHLSDNSIISIDFSNFYLSKTIIKTTSSTTSNNNHLSQINNQSLVNLNNSNFINNQNFEINDGGKFQLFGNSYFENNGDVTLKSNSLFHSPDNGNHVINNYKSWMFSSSSDSSSSSSSSSSSSSNSSSNTTNSTDSYYPTTKLNKFNNFGDLILKDMKLILNQFNTSANSIINLSNSILSIDNNNNNNKTNNSSKTILIHNGNINGNGIIENDIKSSFNSSIGTNSSITKLIVNGSLSQNSNSHFDILINGNDTAGIDFTHILILGEYIASNEEINDNDNDSNNNNNLKRSILSIDGSLSSSAATTTSNTNKTINVYLNNTNTSGNITFLNYSNSNLTNSFTLKLNSYNKTSNSYETINDPCKYQLTQTKSSLSLLISPTSTCRVTDDGKVIITDDNNNGNLIFRNKVIIAVVLCVVFSTAIVGVIIWKKRKINNFIKIKREYFLTKRNSRKGIKENQECTQAENLLD

Target ORF information:

RefSeq Version	XM_630672.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0290283) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_630672.1

ORF Insert Sequence:

ATGATAAAAT TATTTTTAAA ATTATTAATT ATAATTTTAT GTTCGATATG TTTAAATAAT 
ATAATTTTTA ATAATTATTT TGTAAGTGGC CATATATTAA CAACACAAAA TGATACTACC 
AACAATAATA ATAATATAAC TTCATCATCA TCATCTTTAA ATGAATCATC AATTTCTTGT 
GGTGATTTAT CTTTAATGTC AAATCAAACA TATAAAAAAT TTAATTTAAC TTCAACATTG 
GTTCAATCAT CATCATCGTC TTCATCAGTT TCGTCAACAG TTACCAATAC CATTTTGCCA 
CAAAAACTAA ATTCGACAAC ACCATCACTT TCAAAAAATA ATGGTACAAT GATAAGTTCA 
ACAAATACAA CTAAAACATC ATCATCATTT CATTCGTCAT CATCTTCAAC AACAACAACA 
ACATCATCAT CATCATCAGC ATCTGGTTCA ACGACATCTT CGTCTGGTAC AATAGCATCT 
GGTCAATCTT CAAATTGTGT ATATTATAAT AGTAAAACAA CAATAATTAA TTCACCAGAA 
ACAGTAATTG GTGATGTAAG TAAAAAAACA TCAAATACTT TAAATGTTGC ATTCACATTA 
CAATTTAATG GATCATTATC ATTATCAAAT GGTTCAAGTA TGAATATTGG ATGTAATAAT 
AATAGTGGTG AATGTTTACG TACAACAAGT CATTTTCAAA TTGATGGAAT TGTAAATATT 
TTAGATAGTA ATAGTATTTT AGATATTAAT TCAGATTTAA ATATATCAAA AACTGGTATT 
TTACGAACAA ATATATCAGC AGCATCATCA TCATCATCAG CATCAGCATC AAATCAATTT 
GTACCAACAA TGAATGTATT AACAACATCT TCATCATCAT CTTCTTCCAC TTCAAAAGAT 
AGTAATAATA ATAATAATAA TTTACAAGTT AATTCAAAAA CAACATCAAT TAATTCAAAT 
AATTTTTATA ATAATGGTGG ATTATTTAAT ATTGATTTAC CAATATCAAT AAATACAAAC 
CTGTTTAATA TAAATAGTAA AGGTATTATT AATGTAAATT CACCATTAAC TATAAACTCA 
ACACAATCAA TTGTTAAAGG TGGTTCAATT TTAAATATTG ATAATTTATT GAACACTCAA 
GATTCAAAAG GTGGTATGTC AATTTCAGAT GGTTCAAAAG TGAATTTAAA TTCACCATTA 
TCAAATTTAA AAGGATTGTT ATTAGAATTA AGTGACTCAA ACTCAACGTT GAATGCAAAT 
AATCAATCGT TAGTCCAAAT GGATCAGTTC ATAAGTAATG GTGGTAAAAC GATTATAAAT 
AATTCACTAT TAGAGATTAA ATCTCAATTT TTGTTGGGTG ATAAATCAAC AATAAATTTA 
AATAATGAGG GTGGATTACA TGTGAATGGT AATTTCAAAT TGAATGGTAA TGGTACTCTA 
TCTATCAATT CCAATGGGTA TATAGTGAGT GATTCTGGTG ATTTTTATTT AAATGATGAT 
TCTAAAATAA TTGTAAATCA AAGTTCTATT GGAATTCGCT CAAATATTCA TTCAATTGGT 
AATTCAATAT TCCAGAATGA AAATAGTGTT TTTCAATTCA TGGGGTCATC AATTAATTTA 
TCAGATAAGT CTATATTCCT TTTAAAGAAT TGTACAGATG TAAATATGGA ATCTAAAATT 
ACAATGCTAT CTGATAATGC TCAATTCCAA TTATCATCAA ACTCATTCCT AAATACGAAT 
AATCCATTCA ATTTATTAAA TTCATCAATG TTATTAATTA GTGATAATTC AATTCATTCA 
CATAATAATA ATTTAATTCA GTTAAATCAT AATTCTCAAT TGAAAATATC AAGTAATTCA 
AAATTCTCTT TAAATGGATT AGGTTCAAAT TTATTATTAA ATGAAAATTC AATCGTAACA 
ATTGATCAAG GTTCAACTAT TAATTTTAAT AGAGGTTCTT CTTTAAAATT AAATGGTAAT 
ACAATTTTAA ATTTATCAAA TAATTCAAAT ATTTATTTTG AAAATTCAAA TTTACATTTA 
AGTGATAATT CAATAATTTC AATTGATTTT TCAAATTTTT ATTTATCAAA AACTATAATT 
AAAACTACAT CATCAACAAC ATCAAATAAT AATCATTTAT CACAAATTAA TAATCAAAGT 
TTAGTTAATT TAAATAATTC AAATTTTATA AATAATCAAA ATTTTGAAAT TAATGATGGT 
GGTAAATTTC AATTATTTGG TAATTCTTAT TTTGAAAATA ATGGTGATGT AACTTTAAAA 
TCCAATAGTT TATTTCATTC ACCTGATAAT GGTAATCATG TTATAAATAA TTATAAATCT 
TGGATGTTTT CTTCTTCTTC AGATTCTTCT TCTTCTTCCT CCTCCTCTTC ATCATCTTCT 
TCAAATTCTT CTTCAAATAC AACCAATTCA ACAGATTCAT ACTATCCTAC AACAAAACTT 
AATAAATTTA ATAATTTTGG TGATTTAATA TTAAAAGATA TGAAATTAAT ATTAAATCAA 
TTTAATACTT CAGCTAATAG TATTATTAAT TTATCAAATT CAATTTTATC AATTGATAAT 
AATAATAATA ATAATAAAAC AAATAATAGT TCAAAAACTA TATTAATTCA TAATGGTAAT 
ATTAATGGTA ATGGTATTAT TGAAAATGAT ATTAAATCAT CATTCAACTC TTCAATTGGT 
ACAAATTCAT CAATTACAAA GCTAATTGTT AATGGTTCAT TATCTCAAAA TTCAAACTCT 
CATTTCGATA TTTTAATCAA TGGTAATGAT ACTGCTGGTA TTGATTTTAC TCATATTCTT 
ATACTTGGTG AATATATAGC ATCCAATGAA GAAATTAATG ATAATGATAA TGATAGTAAT 
AATAATAATA ATTTAAAAAG ATCGATATTA TCAATTGATG GATCATTATC ATCATCAGCA 
GCAACAACCA CTAGTAATAC AAATAAAACT ATTAATGTTT ATTTAAATAA TACTAATACA 
AGTGGTAATA TTACATTTTT AAATTATTCA AATTCAAATT TAACCAATTC ATTCACATTG 
AAATTAAATT CATACAATAA AACATCAAAC TCTTATGAAA CTATTAACGA TCCCTGTAAA 
TACCAATTAA CACAAACTAA ATCATCACTT AGTTTATTAA TTTCACCAAC TAGTACTTGT 
AGAGTAACTG ATGATGGTAA AGTAATCATT ACTGATGATA ATAATAATGG TAATCTCATC 
TTTAGGAATA AAGTTATTAT CGCTGTAGTT TTATGTGTTG TCTTTAGTAC TGCAATCGTT 
GGTGTTATAA TTTGGAAAAA AAGAAAAATT AATAATTTCA TCAAAATTAA AAGAGAATAT 
TTTTTAACAA AAAGAAATAG TAGAAAGGGG ATCAAAGAGA ATCAAGAGTG TACACAAGCA 
GAAAACCTAT TAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

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DDB_G0290283 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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