DDB_G0280311 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DDB_G0280311
Entrez Gene ID	8622502
Full Name	hypothetical protein
Synonyms	DDBDRAFT_0206508,DDB_0206508
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_636276.1	XP_641368.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB_G0280311 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB_G0280311 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm06190	XM_636276.1 Latest version!	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0280311) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDB_G0280311 ( XM_636276.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_641368.1 Dictyostelium discoideum AX4 hypothetical protein (DDB_G0280311) mRNA, complete cds.

Price & Availability↑

CloneID	ODm06190
Clone ID Related Accession (Same CDS sequence)	XM_636276.1
Accession Version	XM_636276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007089). COMPLETENESS: incomplete on both ends.

ATGTTGACAA ACGAGGATTT GGAACTCCTC AACAACATGA ACACGGGGTC AACAAATATT 
ACCAGTGTAA ATAACAGTAA AAATATTACC AGTAAAAATA ATAATAACAA CAACAATAAC 
AACAACAACA ACAACAACAA CAACAACAAT AATAACAATA TTAAAAACAA TAATAAAAAT 
AATATACTAT ATCGACAATT CCCAATGAAT AGTGGTAGTG GTGAGTACCC AAAAAAAAGA 
AATTTAAAAA GTAACCCAAT GTCATATGAT GACTATGATG ATGAAGAAGA TCCAATTGAA 
TTAGAATTAA AAAAGAAAAT GTTAACAATG TTAAATCAAA ATTCAATACC TCAACAACAC 
CATAATAATG AACACTATAA TGATAATACA TTTTCAGATC AAGATGTAGT ATTTAGTGAT 
AGCGATGGTG GTGGTGGTAA TAACAATAAT AGTTATTATA GTCGTGGTAG TGGAAGTGGT 
CATAGTGATA ATATAACTGA ACTATATAGT AATTTTAATA ATGATCAAAT TTATAATCAA 
ACTTTACAAC AATATAATAA TAAAAGTTTA TTAGCAAAAC AACATAATAG ATCAATCTCT 
GTAAATAATA ATCAACAACA ACAAAATCAA AATCAAAATC AAAATCAAAA TCAAAATCAA 
AATCAAAATC AAAATCAAAA ATCATTTTTA AATACAAGTA ATCCTGGTAT ATCGTTACCA 
TTTGGTAGTC CAAAATTATC TCATATTTCA TCATCAAATA AAGGAGACAA TATGAATTTA 
AATAATAGCG TAAATAGTAC AAATTTATTG AATAGTTATG GTAGTAGTAA TAGTAATAAT 
AATAATAATA ATAATAATAA TAATAGTTAT GAAGAATATT TTAATTTAAA TGATAAATAT 
TTAATATGTG AAAGAGATAG AATTAAATCA TTAACTAAGG AATATAAATT CGATATTATT 
CAAGAGAGAT TAACAATTAA AGAATATAAA TTATATGCAG TAGAGGAATG GATATTTAAA 
TCGAATCATA TGTGGAGTGC GATTTCTTAT ACTGGTAATC CTGATGATAA GGTATTGGTG 
ACATCAATTA GACCAACTCA ACAGTCAAAG GCATTCTTTA ACTTGATTTC AAAACCACCC 
AATTCCACAT TCTACTCTAT CAACACAAGA GAGGGTCAAT TGCTATTGGC AAACTATGGT 
GACTCTTCAC AATATGGTGG CTTGACATTG ATCGATATAG CCGATGGCGA TTTCGATCAG 
CATATATCAG ATTTGAAAAC TAATTTATCT TTGAAAAGAT TAGGTTGTAC AGGTCCAAAA 
TATCAAATAT CTCCAATGAA ACTAACCACT AATTGTAGTG ATAAGTTTAG AAAATTATCT 
GGTTTTCCAA AAATATCAAA CAACTATGTT TGTGATTTCG TTAAAGAATT ACAAGCTGCT 
CTATCTCATT TAAATTATTT ACCTTTATTA ACTAAAATCG ATGGTTGTTA TGATCAAAAA 
ACTATAAATG CATTAAAAGC TTTTCAACTT GATACAAATA AGAAGAAATT ATTAGAAATT 
AATAGTAATA GTGGTACCAC TACTACTACA AATACAAATA CAAATACAAA TACAACTAGT 
TCATTATTAC CAACAGAAGG ATTTTTAGAT CAAAATACAT TTGATGAATT ACAAGAGGAA 
ATTTTGAAAT TAGCAAATAA ATTATATAGT TTAGGTTTTT CACCACCTGA TAGTCCAATC 
AAAAACTATC AAGAATTTAA TGTTGTCATT AAACAATTTC AAGAAAATTT TAGAATTTGT 
CCAGATGCTC CTGGTAAAAA CATTTTAAAT TTTATTGAAA AATTAAATTT AAAAGTACCA 
ATCAATGCAA ATAATTTAAC AAATTCAACA ACTGCTACAA ATACTTTAAA TTCAAATAAT 
ACAAGAAATA GAAGTAAATC TGATTCTGCT GATGATAATA ATAATAATAA TAATTCTTCA 
ACAACTTCCA ATACAACACA TAGAGTTAAT CATTCAAAAC AATCATCTTC AGTTGGTAAT 
AAAATTATAG TTAATCGTAA TACTCGTAAT GGTTCTAATA ATACTACTAA TGCAGCTAGT 
GGTAATATAA TATCAACTGT AAATAATAGT AGAAGTAGTA CAAGTGTTAA TAATAATAGT 
AATAATAATA ATAGTAATAA TAATCAATAT GGTAGTTTTG ATGATATGGA AAGTTTTAAA 
AGAGGTCAAG AGAAAACTGA TGCTGAAATA TTTAGATATT ATGATGATGA TGACGATGAA 
GATGAGGATG AAGATTATTA TGAAGATGTT GAAGAGGATG AATATTATAC TCATGATGAA 
TCTTATGATA AAGATGTTAA ATCTGATACA ACAACCGGTG CTGATGTTGA AGATGATTCA 
AAGAGTGAAG GTTCTTCTTA TTTCAATAGA AATAAATCAT CATCGTCAAC TGGTAATTTA 
CCAGGTGTAT CAACAACAAC AACAACAACA ACATCAACAA CAGCAACAGC AACAAATAAT 
AATACAAAAT CATCAACAGA TGTAACTACA AAAACTACTA CAACCAATAC AAATACAAAT 
TCAAATCCAG CAACAACAAC AACAACAGGT AAAAATATAT TATCACAAAC TCAAAATGAT 
ATAAATAATT TAGAAACTAT TACAGAAGCA GAAGAAAAGG AAAGATTAAA ACAAACAATT 
ATTAAATTAG AAGGATTGGT AGAGAAACAA CAACAACAAT ATTCAAAATT AAAAGATGAT 
TTTAATCAAC TCTATGAAAA TTATTTTGAA TTAAAAGAGA AATCTTTGAA ATCTATTGCA 
GTCATTCAAA CAAATGAAAA AATGTTTGGT GAAATTAATA ATCGTTGGAA TAAATTAACA 
AAAAAAGAAT TACCAGAGAT TGAAGATAAA ATTAATACAA ATGTTGATAT AATTCAGTCC 
TATTCAAACA AAATGAAAAG ATTAGATGAT ATTGTAACAT CAATTCAAAG AAAGAATGAT 
AGAAATATAT TTATCTCATT CTTAATGAGT ATTGGTTCAT TATTGGCAAT TATGTTTGCA 
TATTTGTTAT TATTTGCCAC AAAGATTAAA AGAAATGTTG CTCTTGCTTT GGATCCAATT 
ATTAATCAAA ATAATCATCA AAATGCCAAT AAACCTAAAA CTTCAACTGA TGAGAATATT 
GAAGATTTTG TAATTAAACA AAAGTTAAGA ATAACTCAAG TTATGGATGA TTATGATGAT 
GTAGGAAATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_641368.1
CDS	1..3255
Translation	MLTNEDLELLNNMNTGSTNITSVNNSKNITSKNNNNNNNNNNNNNNNNNNNNNIKNNNKNNILYRQFPMNSGSGEYPKKRNLKSNPMSYDDYDDEEDPIELELKKKMLTMLNQNSIPQQHHNNEHYNDNTFSDQDVVFSDSDGGGGNNNNSYYSRGSGSGHSDNITELYSNFNNDQIYNQTLQQYNNKSLLAKQHNRSISVNNNQQQQNQNQNQNQNQNQNQNQNQKSFLNTSNPGISLPFGSPKLSHISSSNKGDNMNLNNSVNSTNLLNSYGSSNSNNNNNNNNNNSYEEYFNLNDKYLICERDRIKSLTKEYKFDIIQERLTIKEYKLYAVEEWIFKSNHMWSAISYTGNPDDKVLVTSIRPTQQSKAFFNLISKPPNSTFYSINTREGQLLLANYGDSSQYGGLTLIDIADGDFDQHISDLKTNLSLKRLGCTGPKYQISPMKLTTNCSDKFRKLSGFPKISNNYVCDFVKELQAALSHLNYLPLLTKIDGCYDQKTINALKAFQLDTNKKKLLEINSNSGTTTTTNTNTNTNTTSSLLPTEGFLDQNTFDELQEEILKLANKLYSLGFSPPDSPIKNYQEFNVVIKQFQENFRICPDAPGKNILNFIEKLNLKVPINANNLTNSTTATNTLNSNNTRNRSKSDSADDNNNNNNSSTTSNTTHRVNHSKQSSSVGNKIIVNRNTRNGSNNTTNAASGNIISTVNNSRSSTSVNNNSNNNNSNNNQYGSFDDMESFKRGQEKTDAEIFRYYDDDDDEDEDEDYYEDVEEDEYYTHDESYDKDVKSDTTTGADVEDDSKSEGSSYFNRNKSSSSTGNLPGVSTTTTTTTSTTATATNNNTKSSTDVTTKTTTTNTNTNSNPATTTTTGKNILSQTQNDINNLETITEAEEKERLKQTIIKLEGLVEKQQQQYSKLKDDFNQLYENYFELKEKSLKSIAVIQTNEKMFGEINNRWNKLTKKELPEIEDKINTNVDIIQSYSNKMKRLDDIVTSIQRKNDRNIFISFLMSIGSLLAIMFAYLLLFATKIKRNVALALDPIINQNNHQNANKPKTSTDENIEDFVIKQKLRITQVMDDYDDVGNK

Target ORF information:

RefSeq Version	XM_636276.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0280311) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_636276.1

ORF Insert Sequence:

ATGTTGACAA ACGAGGATTT GGAACTCCTC AACAACATGA ACACGGGGTC AACAAATATT 
ACCAGTGTAA ATAACAGTAA AAATATTACC AGTAAAAATA ATAATAACAA CAACAATAAC 
AACAACAACA ACAACAACAA CAACAACAAT AATAACAATA TTAAAAACAA TAATAAAAAT 
AATATACTAT ATCGACAATT CCCAATGAAT AGTGGTAGTG GTGAGTACCC AAAAAAAAGA 
AATTTAAAAA GTAACCCAAT GTCATATGAT GACTATGATG ATGAAGAAGA TCCAATTGAA 
TTAGAATTAA AAAAGAAAAT GTTAACAATG TTAAATCAAA ATTCAATACC TCAACAACAC 
CATAATAATG AACACTATAA TGATAATACA TTTTCAGATC AAGATGTAGT ATTTAGTGAT 
AGCGATGGTG GTGGTGGTAA TAACAATAAT AGTTATTATA GTCGTGGTAG TGGAAGTGGT 
CATAGTGATA ATATAACTGA ACTATATAGT AATTTTAATA ATGATCAAAT TTATAATCAA 
ACTTTACAAC AATATAATAA TAAAAGTTTA TTAGCAAAAC AACATAATAG ATCAATCTCT 
GTAAATAATA ATCAACAACA ACAAAATCAA AATCAAAATC AAAATCAAAA TCAAAATCAA 
AATCAAAATC AAAATCAAAA ATCATTTTTA AATACAAGTA ATCCTGGTAT ATCGTTACCA 
TTTGGTAGTC CAAAATTATC TCATATTTCA TCATCAAATA AAGGAGACAA TATGAATTTA 
AATAATAGCG TAAATAGTAC AAATTTATTG AATAGTTATG GTAGTAGTAA TAGTAATAAT 
AATAATAATA ATAATAATAA TAATAGTTAT GAAGAATATT TTAATTTAAA TGATAAATAT 
TTAATATGTG AAAGAGATAG AATTAAATCA TTAACTAAGG AATATAAATT CGATATTATT 
CAAGAGAGAT TAACAATTAA AGAATATAAA TTATATGCAG TAGAGGAATG GATATTTAAA 
TCGAATCATA TGTGGAGTGC GATTTCTTAT ACTGGTAATC CTGATGATAA GGTATTGGTG 
ACATCAATTA GACCAACTCA ACAGTCAAAG GCATTCTTTA ACTTGATTTC AAAACCACCC 
AATTCCACAT TCTACTCTAT CAACACAAGA GAGGGTCAAT TGCTATTGGC AAACTATGGT 
GACTCTTCAC AATATGGTGG CTTGACATTG ATCGATATAG CCGATGGCGA TTTCGATCAG 
CATATATCAG ATTTGAAAAC TAATTTATCT TTGAAAAGAT TAGGTTGTAC AGGTCCAAAA 
TATCAAATAT CTCCAATGAA ACTAACCACT AATTGTAGTG ATAAGTTTAG AAAATTATCT 
GGTTTTCCAA AAATATCAAA CAACTATGTT TGTGATTTCG TTAAAGAATT ACAAGCTGCT 
CTATCTCATT TAAATTATTT ACCTTTATTA ACTAAAATCG ATGGTTGTTA TGATCAAAAA 
ACTATAAATG CATTAAAAGC TTTTCAACTT GATACAAATA AGAAGAAATT ATTAGAAATT 
AATAGTAATA GTGGTACCAC TACTACTACA AATACAAATA CAAATACAAA TACAACTAGT 
TCATTATTAC CAACAGAAGG ATTTTTAGAT CAAAATACAT TTGATGAATT ACAAGAGGAA 
ATTTTGAAAT TAGCAAATAA ATTATATAGT TTAGGTTTTT CACCACCTGA TAGTCCAATC 
AAAAACTATC AAGAATTTAA TGTTGTCATT AAACAATTTC AAGAAAATTT TAGAATTTGT 
CCAGATGCTC CTGGTAAAAA CATTTTAAAT TTTATTGAAA AATTAAATTT AAAAGTACCA 
ATCAATGCAA ATAATTTAAC AAATTCAACA ACTGCTACAA ATACTTTAAA TTCAAATAAT 
ACAAGAAATA GAAGTAAATC TGATTCTGCT GATGATAATA ATAATAATAA TAATTCTTCA 
ACAACTTCCA ATACAACACA TAGAGTTAAT CATTCAAAAC AATCATCTTC AGTTGGTAAT 
AAAATTATAG TTAATCGTAA TACTCGTAAT GGTTCTAATA ATACTACTAA TGCAGCTAGT 
GGTAATATAA TATCAACTGT AAATAATAGT AGAAGTAGTA CAAGTGTTAA TAATAATAGT 
AATAATAATA ATAGTAATAA TAATCAATAT GGTAGTTTTG ATGATATGGA AAGTTTTAAA 
AGAGGTCAAG AGAAAACTGA TGCTGAAATA TTTAGATATT ATGATGATGA TGACGATGAA 
GATGAGGATG AAGATTATTA TGAAGATGTT GAAGAGGATG AATATTATAC TCATGATGAA 
TCTTATGATA AAGATGTTAA ATCTGATACA ACAACCGGTG CTGATGTTGA AGATGATTCA 
AAGAGTGAAG GTTCTTCTTA TTTCAATAGA AATAAATCAT CATCGTCAAC TGGTAATTTA 
CCAGGTGTAT CAACAACAAC AACAACAACA ACATCAACAA CAGCAACAGC AACAAATAAT 
AATACAAAAT CATCAACAGA TGTAACTACA AAAACTACTA CAACCAATAC AAATACAAAT 
TCAAATCCAG CAACAACAAC AACAACAGGT AAAAATATAT TATCACAAAC TCAAAATGAT 
ATAAATAATT TAGAAACTAT TACAGAAGCA GAAGAAAAGG AAAGATTAAA ACAAACAATT 
ATTAAATTAG AAGGATTGGT AGAGAAACAA CAACAACAAT ATTCAAAATT AAAAGATGAT 
TTTAATCAAC TCTATGAAAA TTATTTTGAA TTAAAAGAGA AATCTTTGAA ATCTATTGCA 
GTCATTCAAA CAAATGAAAA AATGTTTGGT GAAATTAATA ATCGTTGGAA TAAATTAACA 
AAAAAAGAAT TACCAGAGAT TGAAGATAAA ATTAATACAA ATGTTGATAT AATTCAGTCC 
TATTCAAACA AAATGAAAAG ATTAGATGAT ATTGTAACAT CAATTCAAAG AAAGAATGAT 
AGAAATATAT TTATCTCATT CTTAATGAGT ATTGGTTCAT TATTGGCAAT TATGTTTGCA 
TATTTGTTAT TATTTGCCAC AAAGATTAAA AGAAATGTTG CTCTTGCTTT GGATCCAATT 
ATTAATCAAA ATAATCATCA AAATGCCAAT AAACCTAAAA CTTCAACTGA TGAGAATATT 
GAAGATTTTG TAATTAAACA AAAGTTAAGA ATAACTCAAG TTATGGATGA TTATGATGAT 
GTAGGAAATA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

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DDB_G0280311 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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