DDB_G0277109 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DDB_G0277109
Entrez Gene ID	8620842
Full Name	hypothetical protein
Synonyms	DDBDRAFT_0217914,DDB_0217914
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_637878.2	XP_642970.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Glöckner G, Eichinger L, Szafranski K, Pachebat JA, Bankier AT, Dear PH, Lehmann R, Baumgart C, Parra G, Abril JF, Guigó R, Kumpf K, Tunggal B, Cox E, Quail MA, Platzer M, Rosenthal A, Noegel AA,
Nature418(6893)79-85(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB_G0277109 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB_G0277109 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm04606	XM_637878.2 Latest version!	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0277109) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDB_G0277109 ( XM_637878.2 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_642970.2 Dictyostelium discoideum AX4 hypothetical protein (DDB_G0277109) mRNA, complete cds.

Price & Availability↑

CloneID	ODm04606
Clone ID Related Accession (Same CDS sequence)	XM_637878.2
Accession Version	XM_637878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007088). On Aug 7, 2006 this sequence version replaced XM_637878.1. COMPLETENESS: incomplete on both ends.

ATGAAGATAT TTCTATTTTT ATTTTTATTT TTATTATTAA ACTCTGTATT ATTAATAAAA 
GCTCAATCAA TAAAAATATA TGATCTATCA TCATCATTTG ACGAACAAAA TAAATATCCA 
AATTCATCGG AATTATCAAC TTGTAATTTC ATTTTTAATT TCCTATCAGT TGATGATTTA 
AAAACTGGTA TTCCTTTCAA TTATAGTTCA AATACACCAC TTGCAGTATT CAAAGGATCA 
AAAATAAATA ATAGTAGTTC AGAAATCAAT AGCTTTGAAT TTTTAGGAGC AGATTATGGT 
TATTATGAAT TTACAATAAT TGTAACAGCA GGAAGTAAAA GTAGTACTTT TGTTTATAAA 
TTTTCATGTT TAGTAGTTGG TAGTAATCAA GAATTTAATC CATATAATAC TTTTCCATGG 
ATATTAAAAT TTGAAAGTTT AAATGTTTCA TTAAATAAAA TTAAGTTTAT TGAATTAGAT 
ATAAAGTATT CAAAGATTGG TAGAAATACA TTTGCTATCT CAAGTTCATT AGTACAAACA 
AATCCTAAAA GCATTACTAT TGATTTTGCA TCAGGACAAT TACATATAAA ATTAGAATCA 
ATTTTTTTAA ATTATAATAT AAATAGACAT ATAAATATAG CTTTATACCC GAATTCAAAT 
TCAATAATTG AAGAAAAAGG GATAGGGTAT GGACCATTAT TTAAAATTAA TTCAAGTAGT 
TTATTTCCAT ATTATAATAT ACACAATCCA AATTCCCCAA ATTTTCAAAA TACTATATCA 
CCAATATATG GATATCCTGG ACAATATACA TTCATTGGTC AAATAAGGAA TTCGGATAAT 
GGTGAAAACC TTACTTTAAA AACTTTAAAT GGTACTTCTT TTATTGATAT TTCAACTATA 
AAATTAAATT TTAAAGAAAA AAAACAAGAT TCATTTGGAA CACCATCAAG TATTGGTTAT 
TCTCAAATAA ATCCAACATT TTTTACAATT TCATTTAGAA GTATTTCAAA ATTTAAACAT 
TCAACAAATG ATTATTATTT AAATGGTTTA AAAGTAATTT CTTCATCATT CCCATTTGGC 
TTTGCAAATG GATCAAATGA ATCTTTTAAT TATGAAGCTT CATTTTCATT TCCACCATTA 
ACTTTATCAC CATTACAATT TCAAATTAAT TCTGAACCTA TAAAGTTTAT TAATACAATT 
GGTAATTCAG GTTCAAATTT TCAAAAAATT TTATCATATG AAATAAATCA TTTATTTGAA 
TTTAAATATT TAATCAAAAT AACTACAGAT GCATCATTGA ATAATCTTCC AGGTTATATT 
AAAATTAATG AAGTTTATTA TTTTGGATTA GAAAAATGTA CATCTAATGA AATTAATAAT 
ACAACTTTTG AAATTATTGT AGATTTTTTA TTTGGATCTT TAGATGAAGA TGGTGGTGGT 
GGTGGTATTG AAATTTCTGG TACAAATGGA AAGAAATCAT TATATACAAC ATCATCATTT 
TATTCAATTT CTCCACCTTT AAAAATCAAT TATCCTTTTA ATTTTGATAT TGATATTCAA 
AATATTCGAA ATATTAAATT TTTATTTAAT AATATTAATG TTAAAAATAA AAAAAGAGCA 
AATGTAATGT ATTTCAATTA TACAGGTTCA AATATAATAA CGAATAATCA AATAACATTT 
GCATTACATT TAAGTGATAG AACATCATAT ATTCCAGGTG AACCATTAAA AGATAATAAA 
TTTTATTATT CTAAATGGAA TTCAACTATT AAAATGTTTC AAATTGAATT TTTTATAAAT 
GCAAATTCAA TGAGTGGAAT TCTTCCTTGG TTCCTAACAA ATGGTAATCA AAATAGTTTA 
CAAATTAGTA ATTCATTTTT TCCTTTAGAA AATCAATTAA TAATTTTAAA TACAAGTTAT 
GATGGATATG GTCCAATTAT TACATCAATT CAACAAAGGA ATATTTCACA TTCACTAATT 
GTAATATTTG AAATTGAAGA TTTAATTAAT GGATTTAGTC ATGGTGATTT TATAATAAGA 
GGAGAAGTTG ATAATTCAAT TTATAAATTT CAATTAACAG AATTTAATAG AGTATTTGGT 
ACAAATAAGA TTGGTATATA TGAACATACG TTATTAATAA GGAGTAATAA TTTGAAATGT 
TTTAACCAAA CATATGTAAT TAGTGATGTT ATTTTATATG ATAGTCATGG TAATAGAGCC 
GTATTTCAAG AATGGAATAA AGATGGTGGT TATTATTCAA CTACAAATCC ATTTATAAAT 
TATTTATCAA ATCCAAATGT ATTTAAATTT TCATATGATT GTGGTGACTA TATCGATTTA 
ACACCACCTG AACTTTTATC ATTCTCAATT GAGAAATCAT CCTCACAACA AAATCAAAAA 
ACTATTACAA TATTATTTGA AGCAAGAGAT TTAGATAGTG GTTTAAAACC AAACCAACTC 
CCAACACTTT ATGGTATTAC ACCAATGCAA TCAATTCAAA GTATACCAAC TATTGTTTTT 
ATGAATGATA CAAATGTTAA ATATAAATCA ACTATTGAAT TTCCAATTAG CTTTGCTAAT 
ATTTCAACTA TATATTTCTC AATTTATGGT TTCATTAATA ATGGTGGTCA TTTTAGTGGT 
TTCTCATCAG ACTATATCTC TAGACATTTC ATAACATCAG GTGTTGATAT TGGTTTCACT 
AGATATTTAG CAATAGAATC ATCAAGTCAG ATAACATCTG AAGGTGGTGA ACTTTGGGTT 
ATTGGCAGAG AATTTCATTC AACTCTTTCA TTAAAAATAA ATTATTTAGA TGAAAATTTA 
ATAGGTTTCT CTCAACAACA TTTAACACCA ATTAAATCTT ATTCAACAGC TTTATTATTT 
GATGGTATAA AAAAAACAAA TCAAAATTTT ATTATAAAAT TAATAGATTT AAATGGTATT 
GAATCAAATG AATATTTAAT AATACCAATA ATTTTTAATT ATTATGATGA TGATGATAAT 
AATAATAATG ATAATAATAA TACAAGTAGT GAATCACCAA CCACAACACC ATCACCAACA 
ACTCCAATCA ATGAAACTAC ACCAACACCA TCACTACCAA CAACAACACC ATTAGTAACA 
AATCAACCAC AACAATGTAA AGGTACACCA TTATGTGGTT CACCTAAAAA TGGATATTGT 
ACATCTAATG GATGTGTTTG TTTTTCACCA TATACTGGAT TAGATTGCTT ATCAAAGACA 
ATCGTATTGG ATCAACCAAA ATTTAATACA ACTTATCCAA CTTCAGAAAT TCAAGTTAGT 
TTTGAACAAC AACAACAATC ATCATCATCA TCATCGAATT CACTTAATGA AAATTCAGTA 
TTTTTTAAAA CTTTGATTGC AGTTTCATCA TTAAGAGAAA TGGATATTAA TAATAAACCA 
ATTAAAACTT TTAAATTTGG TAAATGGAGT TATTCAAAAA TTGATTCATC AAGTAGTAGA 
TACACAACAA CAATCAATGT TAAATCATTG GATGGCAATA CAACAACACA AACTCAAATA 
GCTACAGTAT TACAATGGTT TGAAAATGAA ACTACCATTC AATTTGCTAA TCAAAACATT 
TCAATGAATC CATCCACTAC TAAATTCACA ATTTCAATCT CAAGTTATCA ATTTGAAAAT 
CCATTACATA ATTTACAATT AGTTATGGAA GCTTCAATTT CAACCACTCA AGATGATGAT 
AAAGTTTGTT CACTTAGAGA ATATGGTGAA ACTAGTTCAG GTGATAATTC AAATTATTTA 
AAAATTAAAG TTGATGATGT CTCGCTATAT GGTAGATTCA TTAGAAGAGC GATCGTTGAT 
ACTTTTGTTA AAAGTGTTGA TAATATTTTA TTAGATTCTT CTTTAAATGC TCTTTCAAAT 
CCAAATGAAT TACAATCTTT TATTGGTATT ACAATTCCAT ATTTCAATGA TAATGTTATA 
ATTGATCCTG TTGATGATAA TTCAGTTTCA GATGAAGAAG ATGCAATTTG TAAAAATAAA 
GAACCATCAA TATCATCAAC CCAACTAGCT GGTATTATTA TAGGTTGTGT TCTATTTGCA 
ATTATAATTG TTGTTATTTC AACCTATTTT ATAATTAAAA AAAGAAAAGA ATTAAAAGTT 
AAATCTGAAA TTAATAACAA AATGAAGGAT TTTGTAAGTG AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_642970.2
CDS	1..4191
Translation	MKIFLFLFLFLLLNSVLLIKAQSIKIYDLSSSFDEQNKYPNSSELSTCNFIFNFLSVDDLKTGIPFNYSSNTPLAVFKGSKINNSSSEINSFEFLGADYGYYEFTIIVTAGSKSSTFVYKFSCLVVGSNQEFNPYNTFPWILKFESLNVSLNKIKFIELDIKYSKIGRNTFAISSSLVQTNPKSITIDFASGQLHIKLESIFLNYNINRHINIALYPNSNSIIEEKGIGYGPLFKINSSSLFPYYNIHNPNSPNFQNTISPIYGYPGQYTFIGQIRNSDNGENLTLKTLNGTSFIDISTIKLNFKEKKQDSFGTPSSIGYSQINPTFFTISFRSISKFKHSTNDYYLNGLKVISSSFPFGFANGSNESFNYEASFSFPPLTLSPLQFQINSEPIKFINTIGNSGSNFQKILSYEINHLFEFKYLIKITTDASLNNLPGYIKINEVYYFGLEKCTSNEINNTTFEIIVDFLFGSLDEDGGGGGIEISGTNGKKSLYTTSSFYSISPPLKINYPFNFDIDIQNIRNIKFLFNNINVKNKKRANVMYFNYTGSNIITNNQITFALHLSDRTSYIPGEPLKDNKFYYSKWNSTIKMFQIEFFINANSMSGILPWFLTNGNQNSLQISNSFFPLENQLIILNTSYDGYGPIITSIQQRNISHSLIVIFEIEDLINGFSHGDFIIRGEVDNSIYKFQLTEFNRVFGTNKIGIYEHTLLIRSNNLKCFNQTYVISDVILYDSHGNRAVFQEWNKDGGYYSTTNPFINYLSNPNVFKFSYDCGDYIDLTPPELLSFSIEKSSSQQNQKTITILFEARDLDSGLKPNQLPTLYGITPMQSIQSIPTIVFMNDTNVKYKSTIEFPISFANISTIYFSIYGFINNGGHFSGFSSDYISRHFITSGVDIGFTRYLAIESSSQITSEGGELWVIGREFHSTLSLKINYLDENLIGFSQQHLTPIKSYSTALLFDGIKKTNQNFIIKLIDLNGIESNEYLIIPIIFNYYDDDDNNNNDNNNTSSESPTTTPSPTTPINETTPTPSLPTTTPLVTNQPQQCKGTPLCGSPKNGYCTSNGCVCFSPYTGLDCLSKTIVLDQPKFNTTYPTSEIQVSFEQQQQSSSSSSNSLNENSVFFKTLIAVSSLREMDINNKPIKTFKFGKWSYSKIDSSSSRYTTTINVKSLDGNTTTQTQIATVLQWFENETTIQFANQNISMNPSTTKFTISISSYQFENPLHNLQLVMEASISTTQDDDKVCSLREYGETSSGDNSNYLKIKVDDVSLYGRFIRRAIVDTFVKSVDNILLDSSLNALSNPNELQSFIGITIPYFNDNVIIDPVDDNSVSDEEDAICKNKEPSISSTQLAGIIIGCVLFAIIIVVISTYFIIKKRKELKVKSEINNKMKDFVSENK

Target ORF information:

RefSeq Version	XM_637878.2
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0277109) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_637878.2

ORF Insert Sequence:

ATGAAGATAT TTCTATTTTT ATTTTTATTT TTATTATTAA ACTCTGTATT ATTAATAAAA 
GCTCAATCAA TAAAAATATA TGATCTATCA TCATCATTTG ACGAACAAAA TAAATATCCA 
AATTCATCGG AATTATCAAC TTGTAATTTC ATTTTTAATT TCCTATCAGT TGATGATTTA 
AAAACTGGTA TTCCTTTCAA TTATAGTTCA AATACACCAC TTGCAGTATT CAAAGGATCA 
AAAATAAATA ATAGTAGTTC AGAAATCAAT AGCTTTGAAT TTTTAGGAGC AGATTATGGT 
TATTATGAAT TTACAATAAT TGTAACAGCA GGAAGTAAAA GTAGTACTTT TGTTTATAAA 
TTTTCATGTT TAGTAGTTGG TAGTAATCAA GAATTTAATC CATATAATAC TTTTCCATGG 
ATATTAAAAT TTGAAAGTTT AAATGTTTCA TTAAATAAAA TTAAGTTTAT TGAATTAGAT 
ATAAAGTATT CAAAGATTGG TAGAAATACA TTTGCTATCT CAAGTTCATT AGTACAAACA 
AATCCTAAAA GCATTACTAT TGATTTTGCA TCAGGACAAT TACATATAAA ATTAGAATCA 
ATTTTTTTAA ATTATAATAT AAATAGACAT ATAAATATAG CTTTATACCC GAATTCAAAT 
TCAATAATTG AAGAAAAAGG GATAGGGTAT GGACCATTAT TTAAAATTAA TTCAAGTAGT 
TTATTTCCAT ATTATAATAT ACACAATCCA AATTCCCCAA ATTTTCAAAA TACTATATCA 
CCAATATATG GATATCCTGG ACAATATACA TTCATTGGTC AAATAAGGAA TTCGGATAAT 
GGTGAAAACC TTACTTTAAA AACTTTAAAT GGTACTTCTT TTATTGATAT TTCAACTATA 
AAATTAAATT TTAAAGAAAA AAAACAAGAT TCATTTGGAA CACCATCAAG TATTGGTTAT 
TCTCAAATAA ATCCAACATT TTTTACAATT TCATTTAGAA GTATTTCAAA ATTTAAACAT 
TCAACAAATG ATTATTATTT AAATGGTTTA AAAGTAATTT CTTCATCATT CCCATTTGGC 
TTTGCAAATG GATCAAATGA ATCTTTTAAT TATGAAGCTT CATTTTCATT TCCACCATTA 
ACTTTATCAC CATTACAATT TCAAATTAAT TCTGAACCTA TAAAGTTTAT TAATACAATT 
GGTAATTCAG GTTCAAATTT TCAAAAAATT TTATCATATG AAATAAATCA TTTATTTGAA 
TTTAAATATT TAATCAAAAT AACTACAGAT GCATCATTGA ATAATCTTCC AGGTTATATT 
AAAATTAATG AAGTTTATTA TTTTGGATTA GAAAAATGTA CATCTAATGA AATTAATAAT 
ACAACTTTTG AAATTATTGT AGATTTTTTA TTTGGATCTT TAGATGAAGA TGGTGGTGGT 
GGTGGTATTG AAATTTCTGG TACAAATGGA AAGAAATCAT TATATACAAC ATCATCATTT 
TATTCAATTT CTCCACCTTT AAAAATCAAT TATCCTTTTA ATTTTGATAT TGATATTCAA 
AATATTCGAA ATATTAAATT TTTATTTAAT AATATTAATG TTAAAAATAA AAAAAGAGCA 
AATGTAATGT ATTTCAATTA TACAGGTTCA AATATAATAA CGAATAATCA AATAACATTT 
GCATTACATT TAAGTGATAG AACATCATAT ATTCCAGGTG AACCATTAAA AGATAATAAA 
TTTTATTATT CTAAATGGAA TTCAACTATT AAAATGTTTC AAATTGAATT TTTTATAAAT 
GCAAATTCAA TGAGTGGAAT TCTTCCTTGG TTCCTAACAA ATGGTAATCA AAATAGTTTA 
CAAATTAGTA ATTCATTTTT TCCTTTAGAA AATCAATTAA TAATTTTAAA TACAAGTTAT 
GATGGATATG GTCCAATTAT TACATCAATT CAACAAAGGA ATATTTCACA TTCACTAATT 
GTAATATTTG AAATTGAAGA TTTAATTAAT GGATTTAGTC ATGGTGATTT TATAATAAGA 
GGAGAAGTTG ATAATTCAAT TTATAAATTT CAATTAACAG AATTTAATAG AGTATTTGGT 
ACAAATAAGA TTGGTATATA TGAACATACG TTATTAATAA GGAGTAATAA TTTGAAATGT 
TTTAACCAAA CATATGTAAT TAGTGATGTT ATTTTATATG ATAGTCATGG TAATAGAGCC 
GTATTTCAAG AATGGAATAA AGATGGTGGT TATTATTCAA CTACAAATCC ATTTATAAAT 
TATTTATCAA ATCCAAATGT ATTTAAATTT TCATATGATT GTGGTGACTA TATCGATTTA 
ACACCACCTG AACTTTTATC ATTCTCAATT GAGAAATCAT CCTCACAACA AAATCAAAAA 
ACTATTACAA TATTATTTGA AGCAAGAGAT TTAGATAGTG GTTTAAAACC AAACCAACTC 
CCAACACTTT ATGGTATTAC ACCAATGCAA TCAATTCAAA GTATACCAAC TATTGTTTTT 
ATGAATGATA CAAATGTTAA ATATAAATCA ACTATTGAAT TTCCAATTAG CTTTGCTAAT 
ATTTCAACTA TATATTTCTC AATTTATGGT TTCATTAATA ATGGTGGTCA TTTTAGTGGT 
TTCTCATCAG ACTATATCTC TAGACATTTC ATAACATCAG GTGTTGATAT TGGTTTCACT 
AGATATTTAG CAATAGAATC ATCAAGTCAG ATAACATCTG AAGGTGGTGA ACTTTGGGTT 
ATTGGCAGAG AATTTCATTC AACTCTTTCA TTAAAAATAA ATTATTTAGA TGAAAATTTA 
ATAGGTTTCT CTCAACAACA TTTAACACCA ATTAAATCTT ATTCAACAGC TTTATTATTT 
GATGGTATAA AAAAAACAAA TCAAAATTTT ATTATAAAAT TAATAGATTT AAATGGTATT 
GAATCAAATG AATATTTAAT AATACCAATA ATTTTTAATT ATTATGATGA TGATGATAAT 
AATAATAATG ATAATAATAA TACAAGTAGT GAATCACCAA CCACAACACC ATCACCAACA 
ACTCCAATCA ATGAAACTAC ACCAACACCA TCACTACCAA CAACAACACC ATTAGTAACA 
AATCAACCAC AACAATGTAA AGGTACACCA TTATGTGGTT CACCTAAAAA TGGATATTGT 
ACATCTAATG GATGTGTTTG TTTTTCACCA TATACTGGAT TAGATTGCTT ATCAAAGACA 
ATCGTATTGG ATCAACCAAA ATTTAATACA ACTTATCCAA CTTCAGAAAT TCAAGTTAGT 
TTTGAACAAC AACAACAATC ATCATCATCA TCATCGAATT CACTTAATGA AAATTCAGTA 
TTTTTTAAAA CTTTGATTGC AGTTTCATCA TTAAGAGAAA TGGATATTAA TAATAAACCA 
ATTAAAACTT TTAAATTTGG TAAATGGAGT TATTCAAAAA TTGATTCATC AAGTAGTAGA 
TACACAACAA CAATCAATGT TAAATCATTG GATGGCAATA CAACAACACA AACTCAAATA 
GCTACAGTAT TACAATGGTT TGAAAATGAA ACTACCATTC AATTTGCTAA TCAAAACATT 
TCAATGAATC CATCCACTAC TAAATTCACA ATTTCAATCT CAAGTTATCA ATTTGAAAAT 
CCATTACATA ATTTACAATT AGTTATGGAA GCTTCAATTT CAACCACTCA AGATGATGAT 
AAAGTTTGTT CACTTAGAGA ATATGGTGAA ACTAGTTCAG GTGATAATTC AAATTATTTA 
AAAATTAAAG TTGATGATGT CTCGCTATAT GGTAGATTCA TTAGAAGAGC GATCGTTGAT 
ACTTTTGTTA AAAGTGTTGA TAATATTTTA TTAGATTCTT CTTTAAATGC TCTTTCAAAT 
CCAAATGAAT TACAATCTTT TATTGGTATT ACAATTCCAT ATTTCAATGA TAATGTTATA 
ATTGATCCTG TTGATGATAA TTCAGTTTCA GATGAAGAAG ATGCAATTTG TAAAAATAAA 
GAACCATCAA TATCATCAAC CCAACTAGCT GGTATTATTA TAGGTTGTGT TCTATTTGCA 
ATTATAATTG TTGTTATTTC AACCTATTTT ATAATTAAAA AAAGAAAAGA ATTAAAAGTT 
AAATCTGAAA TTAATAACAA AATGAAGGAT TTTGTAAGTG AAAATAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Nature418(6893)79-85(2002 Jul)
Glöckner G,Eichinger L,Szafranski K,Pachebat JA,Bankier AT,Dear PH,Lehmann R,Baumgart C,Parra G,Abril JF,Guigó R,Kumpf K,Tunggal B,Cox E,Quail MA,Platzer M,Rosenthal A,Noegel AA,

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DDB_G0277109 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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