DDB_G0273829 cDNA ORF clone, Dictyostelium discoideum AX4

Gene
Clones
gRNAs

Summary

Gene Symbol	DDB_G0273829
Entrez Gene ID	8619157
Full Name	hypothetical protein
Synonyms	DDBDRAFT_0217328,DDB_0217328
Gene Type	protein-coding
Organism	Dictyostelium discoideum AX4

mRNA and Protein(s)

mRNA	Protein	Name
XM_639439.1	XP_644531.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the social amoeba Dictyostelium discoideum.
Eichinger L, Pachebat JA, Glöckner G, Rajandream MA, Sucgang R, Berriman M, Song J, Olsen R, Szafranski K, Xu Q, Tunggal B, Kummerfeld S, Madera M, Konfortov BA, Rivero F, Bankier AT, Lehmann R, Hamlin N, Davies R, Gaudet P, Fey P, Pilcher K, Chen G, Saunders D, Sodergren E, Davis P, Kerhornou A, Nie X, Hall N, Anjard C, Hemphill L, Bason N, Farbrother P, Desany B, Just E, Morio T, Rost R, Churcher C, Cooper J, Haydock S, van Driessche N, Cronin A, Goodhead I, Muzny D, Mourier T, Pain A, Lu M, Harper D, Lindsay R, Hauser H, James K, Quiles M, Madan Babu M, Saito T, Buchrieser C, Wardroper A, Felder M, Thangavelu M, Johnson D, Knights A, Loulseged H, Mungall K, Oliver K, Price C, Quail MA, Urushihara H, Hernandez J, Rabbinowitsch E, Steffen D, Sanders M, Ma J, Kohara Y, Sharp S, Simmonds M, Spiegler S, Tivey A, Sugano S, White B, Walker D, Woodward J, Winckler T, Tanaka Y, Shaulsky G, Schleicher M, Weinstock G, Rosenthal A, Cox EC, Chisholm RL, Gibbs R, Loomis WF, Platzer M, Kay RR, Williams J, Dear PH, Noegel AA, Barrell B, Kuspa A
Nature435(7038)43-57(2005 May)

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Glöckner G, Eichinger L, Szafranski K, Pachebat JA, Bankier AT, Dear PH, Lehmann R, Baumgart C, Parra G, Abril JF, Guigó R, Kumpf K, Tunggal B, Cox E, Quail MA, Platzer M, Rosenthal A, Noegel AA,
Nature418(6893)79-85(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDB_G0273829 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDB_G0273829 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODm02980	XM_639439.1 Latest version!	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0273829) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDB_G0273829 ( XM_639439.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_644531.1 Dictyostelium discoideum AX4 hypothetical protein (DDB_G0273829) mRNA, complete cds.

Price & Availability↑

CloneID	ODm02980
Clone ID Related Accession (Same CDS sequence)	XM_639439.1
Accession Version	XM_639439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1264608000000
Organism	Dictyostelium discoideum AX4
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007088). COMPLETENESS: incomplete on both ends.

ATGAAAATTT CAATTTTATT AATTATCTGT ATAATTTATT TATCAATTTC AAATGTTAAA 
TCATATGGAG AATCTGATAA TTCTGGTTAT CCAAATTATC AAGAAAGAGA AGCTCACACA 
ATGATGAATG TTGTTCGTCA ATTGCCAACA AAATATAGAG ATACTTTTAT GACTGGATAT 
TCTGGTTACC AAAATTTATT TACCACATCA ACAGCTTTAC CACCAACTAC ACCAGTTTAT 
TATAATCAAA CATTAGGTTA TCTTGCAAGA TCACACTCTT CAGAAATGGG CATTAAAGGT 
TATTTTAGTC ATAACAGCTA TGATGGAACT TCTTGGAGTA CTCGTTTCGA TTCCTTTGTT 
GCCGGTTGTC AATCTGCAGG AAATTCAATT GCTGAGAATA TTGCAGCAGG AGGGGTATCA 
GGAATCGGAA CAACTAGTCA ATTACTTTGT GATGCTGTTT CTGGTGTTTG TGTAGTTGAT 
ACAAGCTCTG ATGCTGGACA CCGTAAGAAT ATAATGGGAA ACTATAAAAT CGTTGGTATT 
GGTTTTTACT ATAACTCAAG TAGTCAATAT GGAAATTATT GGACCCAAGA TTTTGATAAT 
GGTATTTGTG GATTACCAAC TACTCCAATT TATTCAGGAT TCCATACTTT CTTCTTTGGA 
GCAAATGCAC CAAATTATTA TTTATCATAT TATAGTTCAA CAGATGCAGC AAGTAAAGTA 
TCATTTATTT TTGAAAATGG AACATCAATT GATATGACAT TATTTGGTGG TACAGCCAAT 
TCTGGTATTT ATACAACTTC AACAGTTTAT CAAGTGTGTG CTAAATATTA TTTCTCTGTC 
ACAAGTGCAA GTGGTAAAAC ATATAGGTAT CCAGATACTG GTTATTTACA ATTAACTAAA 
GCCAGTACAT GTGCTTCTTG GGTTGCCTCT TCATCTAGTT CAACCACATC TACTACATCA 
ACCTCATCAA CTACCTCCAA ACCAACTACT ACATCAACCA CATCAACTAC ATCAACAACA 
TCAACAACAT CAACTACCTC TAAACCAACA ACTACATCAA CAACATCAAC TACATCAACT 
ACCTCTAAAC CAACAACCAC ATCAACTACA TCAACTACAT CAACTACATC AACTACTTCC 
AAACCAACTA CTACATCAAC CACATCAACT ACTTCCAAAA CAACTACTGG TTCATCAACA 
ACAACAACTG GTTCATCAAC AACAACTGGT TCATCAACAA CCACATCATC ACCATCAACT 
ACAGATACTC AAACAGAATC TCTTGAGGAT GGATCAACAC CATCAAGCTC TAACACTGTA 
AAATCATCAT TATTAATTGT ATTGGTATCA CTTGTATTAT TAATTGCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_644531.1
CDS	1..1374
Translation	MKISILLIICIIYLSISNVKSYGESDNSGYPNYQEREAHTMMNVVRQLPTKYRDTFMTGYSGYQNLFTTSTALPPTTPVYYNQTLGYLARSHSSEMGIKGYFSHNSYDGTSWSTRFDSFVAGCQSAGNSIAENIAAGGVSGIGTTSQLLCDAVSGVCVVDTSSDAGHRKNIMGNYKIVGIGFYYNSSSQYGNYWTQDFDNGICGLPTTPIYSGFHTFFFGANAPNYYLSYYSSTDAASKVSFIFENGTSIDMTLFGGTANSGIYTTSTVYQVCAKYYFSVTSASGKTYRYPDTGYLQLTKASTCASWVASSSSSTTSTTSTSSTTSKPTTTSTTSTTSTTSTTSTTSKPTTTSTTSTTSTTSKPTTTSTTSTTSTTSTTSKPTTTSTTSTTSKTTTGSSTTTTGSSTTTGSSTTTSSPSTTDTQTESLEDGSTPSSSNTVKSSLLIVLVSLVLLIAF

Target ORF information:

RefSeq Version	XM_639439.1
Organism	Dictyostelium discoideum AX4
Definition	Dictyostelium discoideum AX4 hypothetical protein (DDB_G0273829) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_639439.1

ORF Insert Sequence:

ATGAAAATTT CAATTTTATT AATTATCTGT ATAATTTATT TATCAATTTC AAATGTTAAA 
TCATATGGAG AATCTGATAA TTCTGGTTAT CCAAATTATC AAGAAAGAGA AGCTCACACA 
ATGATGAATG TTGTTCGTCA ATTGCCAACA AAATATAGAG ATACTTTTAT GACTGGATAT 
TCTGGTTACC AAAATTTATT TACCACATCA ACAGCTTTAC CACCAACTAC ACCAGTTTAT 
TATAATCAAA CATTAGGTTA TCTTGCAAGA TCACACTCTT CAGAAATGGG CATTAAAGGT 
TATTTTAGTC ATAACAGCTA TGATGGAACT TCTTGGAGTA CTCGTTTCGA TTCCTTTGTT 
GCCGGTTGTC AATCTGCAGG AAATTCAATT GCTGAGAATA TTGCAGCAGG AGGGGTATCA 
GGAATCGGAA CAACTAGTCA ATTACTTTGT GATGCTGTTT CTGGTGTTTG TGTAGTTGAT 
ACAAGCTCTG ATGCTGGACA CCGTAAGAAT ATAATGGGAA ACTATAAAAT CGTTGGTATT 
GGTTTTTACT ATAACTCAAG TAGTCAATAT GGAAATTATT GGACCCAAGA TTTTGATAAT 
GGTATTTGTG GATTACCAAC TACTCCAATT TATTCAGGAT TCCATACTTT CTTCTTTGGA 
GCAAATGCAC CAAATTATTA TTTATCATAT TATAGTTCAA CAGATGCAGC AAGTAAAGTA 
TCATTTATTT TTGAAAATGG AACATCAATT GATATGACAT TATTTGGTGG TACAGCCAAT 
TCTGGTATTT ATACAACTTC AACAGTTTAT CAAGTGTGTG CTAAATATTA TTTCTCTGTC 
ACAAGTGCAA GTGGTAAAAC ATATAGGTAT CCAGATACTG GTTATTTACA ATTAACTAAA 
GCCAGTACAT GTGCTTCTTG GGTTGCCTCT TCATCTAGTT CAACCACATC TACTACATCA 
ACCTCATCAA CTACCTCCAA ACCAACTACT ACATCAACCA CATCAACTAC ATCAACAACA 
TCAACAACAT CAACTACCTC TAAACCAACA ACTACATCAA CAACATCAAC TACATCAACT 
ACCTCTAAAC CAACAACCAC ATCAACTACA TCAACTACAT CAACTACATC AACTACTTCC 
AAACCAACTA CTACATCAAC CACATCAACT ACTTCCAAAA CAACTACTGG TTCATCAACA 
ACAACAACTG GTTCATCAAC AACAACTGGT TCATCAACAA CCACATCATC ACCATCAACT 
ACAGATACTC AAACAGAATC TCTTGAGGAT GGATCAACAC CATCAAGCTC TAACACTGTA 
AAATCATCAT TATTAATTGT ATTGGTATCA CTTGTATTAT TAATTGCTTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the social amoeba Dictyostelium discoideum.
Nature435(7038)43-57(2005 May)
Eichinger L,Pachebat JA,Glöckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.
Nature418(6893)79-85(2002 Jul)
Glöckner G,Eichinger L,Szafranski K,Pachebat JA,Bankier AT,Dear PH,Lehmann R,Baumgart C,Parra G,Abril JF,Guigó R,Kumpf K,Tunggal B,Cox E,Quail MA,Platzer M,Rosenthal A,Noegel AA,

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DDB_G0273829 cDNA ORF clone, Dictyostelium discoideum AX4

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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