dvl3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dvl3a
Entrez Gene ID	80972
Full Name	dishevelled segment polarity protein 3a
Synonyms	dvl3,wu:fb75f04,wu:fi22h02,wu:fp61b09
General protein information	Preferred Names dishevelled segment polarity protein 3a Names dishevelled segment polarity protein 3a dishevelled 3 dishevelled, dsh homolog 3 dishevelled, dsh homolog 3a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131757.2	NP_571832.2	dishevelled segment polarity protein 3a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mutational analysis of dishevelled genes in zebrafish reveals distinct functions in embryonic patterning and gastrulation cell movements.
Xing YY, Cheng XN, Li YL, Zhang C, Saquet A, Liu YY, Shao M, Shi DL
PLoS genetics14(8)e1007551(2018 08)

CFTR mutation enhances Dishevelled degradation and results in impairment of Wnt-dependent hematopoiesis.
Sun H, Wang Y, Zhang J, Chen Y, Liu Y, Lin Z, Liu M, Sheng K, Liao H, Tsang KS, Zhang X, Jiang X, Xu W, Mao M, Chan HC
Cell death & disease9(3)275(2018 02)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Hsu CH, Lin JS, Po Lai K, Li JW, Chan TF, You MS, Tse WK, Jiang YJ
Scientific reports510673(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dvl3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dvl3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39439	NM_131757.2 Latest version!	Danio rerio dishevelled segment polarity protein 3a (dvl3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dvl3a ( NM_131757.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571832.2 Danio rerio dishevelled segment polarity protein 3a (dvl3a), mRNA.

Price & Availability↑

CloneID	ODa39439
Clone ID Related Accession (Same CDS sequence)	NM_131757.2
Accession Version	NM_131757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548345600000
Organism	Danio rerio(zebrafish)
Product	dishevelled segment polarity protein 3a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU638697.5, EH447762.1, EB922958.1 and CU633992.6. On Jun 17, 2015 this sequence version replaced NM_131757.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01009520.1, GFIL01009066.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA CTAAAGTTAT CTACCATCTC GACGACCAGG AAACTCCATA CCTGGTCAAA 
CTGCCCATCC CTGCCGAAAG GGTCACCCTT TTGGACCTGA AAAATGCTCT CAAGAAACCA 
AATTACAAGT TTTTCTTTAA ATCAATGGAC GATGACTTTG GGGTGGTCAA GGAAGAAATC 
ACAGACGACA ATGCAAAGCT TCCCTGTTAC AATGGCCGTG TGATTTGTTG GCTGGTGTCA 
TCAGACGGCT CCCACTCAGA TGGATGTTCA GTAGCAGAGA GTCAGTCTGA GCGGCCGCCA 
TCTCACGAGA GAAGCCAAGG CATTGGAGAT TCCAGACCCC CATCCTTCCA TCCAAAGGCT 
GCAGGCAGCC GGCATGGTGT GGATGATGAG ACGGAGACCG ATTCGGTTGT GTCACACAGG 
AGAGACAGAG ACAGGGACAG ATCGCGGAGG AAACACTCAC ATGAGCACTC TGGAGGAAAG 
CAGAATGGCT ACTCGCGGGG AGGCAGAGGC ATGGATCTGG GAGGCTATGA CATCTGTGAC 
AGCCGTTCCT CCCTCATGAG CAGTGAACTG GAATCGAGCA GCTGTTTTGA CTCGGATGAC 
GGCTCCACCA GCCGTTTCAG CAGTGTTACA GAGCAAAGTG GCTCATCCAA ACTAATGACA 
AAAAAACCCC GGCGGCGGAG GCGCAAGACC AGAGCCCAGC AAATGGAAAG GTCTTCATCA 
TTCAGTAGCA TCACTGACTC CACTATGTCC CTGAATATCA TCACTGTCAC TTTAAATATG 
GAGAAGTATA ACTTTCTGGG CATCAGCATC GTGGGTCAGA GTAATGAGCG GGGTGATGGA 
GGAATCTACA TCGGCTCCAT AATGAAGGGT GGAGCAGTGG CGGCTGATGG GCGGATCGAG 
CCCGGTGACA TGCTGCTGCA GGTGAACGAC ATCAACTTTG AGAACATGTG CAACGATGAT 
GCCGTGCGAG TGCTGAGGGA GATTGTGCAC AAGCCTGGAC CTGTATCGCT TACTGTGGCC 
AAATGCTGGG ACCCAAACCC TAACAGTTGC TTTGCTTTGC CGAGGAGTGA ACCCATCCGG 
CCTATTGACC CTGCAGCCTG GGTGTCCCAT ACAGCAGCCA TGACGGGTGT CTATCCTCCC 
TACGGCATGA GTCCATCCAT GAGCACAGTC ACCTCCACCA GCTCCTCCAT TTCCAGCTCC 
ATCCCCGAGA CCGAGAGATT TGATGACTAC CACCTGTCCG TCCACAGCGA CATGGCAACA 
GTTGCAAAAG CAATGGCCTG TCCAGACTCG GGATTGGAAG TTCGAGACCG AATGTGGCTG 
AAGATCACTA TAGCCAATGC TTTCATAGGT TCGGATGTGG TGGACTGGCT TTTTCATCAT 
GTGGAGGGGT TCACAGATCG CCGTGAAGCA CGAAAGTATG CCAGCAACCT GTTGAAGGCT 
GGCTTCATCC GCCACACGGT CAACAAAATC ACCTTCTCAG AGCAGTGCTA CTACATCTTT 
GGAGATCTTT CTGGCAACAT GGCCCATCTC TCTCTGCAAG ACCACGACGG GTCAAGTGGA 
GCATCTGATC AGGACACTCT TGCTCCTTTG CTTCACCCTG CTGAAGCTCC CTGGCCCGCA 
GCGTTCCCGT ACCAGTACCC AGCAGCCCAT TCACACCTGG AGGAAGCTGG AGGAGGCAGC 
GCAGGCAGTC AGCAAAGTGA AGGGAGCAGC GGTTCAAACT GCAGCTGGAG CAGGACTGAG 
GGCAAAACCG CAGCCGGAGA CTTCAGGTTA GGAGGCGGAA GCGAAATGGG TGACGCTCAC 
GAGTTTGACT TCCGCCGAGA CCGAGCACCC AGCGAACGCT CGGGCGTGCC CCCCAGCGAG 
GGCAGCGTGC GGAGCTCTCG GTCTCACAGC TACAGCCTGA AGCAGGGGTC TGCACGAGGT 
GGCCCGGGGT CACCATCCGG CCGACATCTT GCCCACATTC CCCCTGAACT CACTGGCTCT 
CGCCGCTCCT GCAACACAGC CAACGGGGAG TTCTTCGTCG ACATCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571832.2
CDS	369..2399
Translation	MGETKVIYHLDDQETPYLVKLPIPAERVTLLDLKNALKKPNYKFFFKSMDDDFGVVKEEITDDNAKLPCYNGRVICWLVSSDGSHSDGCSVAESQSERPPSHERSQGIGDSRPPSFHPKAAGSRHGVDDETETDSVVSHRRDRDRDRSRRKHSHEHSGGKQNGYSRGGRGMDLGGYDICDSRSSLMSSELESSSCFDSDDGSTSRFSSVTEQSGSSKLMTKKPRRRRRKTRAQQMERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDINFENMCNDDAVRVLREIVHKPGPVSLTVAKCWDPNPNSCFALPRSEPIRPIDPAAWVSHTAAMTGVYPPYGMSPSMSTVTSTSSSISSSIPETERFDDYHLSVHSDMATVAKAMACPDSGLEVRDRMWLKITIANAFIGSDVVDWLFHHVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLSGNMAHLSLQDHDGSSGASDQDTLAPLLHPAEAPWPAAFPYQYPAAHSHLEEAGGGSAGSQQSEGSSGSNCSWSRTEGKTAAGDFRLGGGSEMGDAHEFDFRRDRAPSERSGVPPSEGSVRSSRSHSYSLKQGSARGGPGSPSGRHLAHIPPELTGSRRSCNTANGEFFVDIM

Target ORF information:

RefSeq Version	NM_131757.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled segment polarity protein 3a (dvl3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131757.2

ORF Insert Sequence:

ATGGGGGAGA CTAAAGTTAT CTACCATCTC GACGACCAGG AAACTCCATA CCTGGTCAAA 
CTGCCCATCC CTGCCGAAAG GGTCACCCTT TTGGACCTGA AAAATGCTCT CAAGAAACCA 
AATTACAAGT TTTTCTTTAA ATCAATGGAC GATGACTTTG GGGTGGTCAA GGAAGAAATC 
ACAGACGACA ATGCAAAGCT TCCCTGTTAC AATGGCCGTG TGATTTGTTG GCTGGTGTCA 
TCAGACGGCT CCCACTCAGA TGGATGTTCA GTAGCAGAGA GTCAGTCTGA GCGGCCGCCA 
TCTCACGAGA GAAGCCAAGG CATTGGAGAT TCCAGACCCC CATCCTTCCA TCCAAAGGCT 
GCAGGCAGCC GGCATGGTGT GGATGATGAG ACGGAGACCG ATTCGGTTGT GTCACACAGG 
AGAGACAGAG ACAGGGACAG ATCGCGGAGG AAACACTCAC ATGAGCACTC TGGAGGAAAG 
CAGAATGGCT ACTCGCGGGG AGGCAGAGGC ATGGATCTGG GAGGCTATGA CATCTGTGAC 
AGCCGTTCCT CCCTCATGAG CAGTGAACTG GAATCGAGCA GCTGTTTTGA CTCGGATGAC 
GGCTCCACCA GCCGTTTCAG CAGTGTTACA GAGCAAAGTG GCTCATCCAA ACTAATGACA 
AAAAAACCCC GGCGGCGGAG GCGCAAGACC AGAGCCCAGC AAATGGAAAG GTCTTCATCA 
TTCAGTAGCA TCACTGACTC CACTATGTCC CTGAATATCA TCACTGTCAC TTTAAATATG 
GAGAAGTATA ACTTTCTGGG CATCAGCATC GTGGGTCAGA GTAATGAGCG GGGTGATGGA 
GGAATCTACA TCGGCTCCAT AATGAAGGGT GGAGCAGTGG CGGCTGATGG GCGGATCGAG 
CCCGGTGACA TGCTGCTGCA GGTGAACGAC ATCAACTTTG AGAACATGTG CAACGATGAT 
GCCGTGCGAG TGCTGAGGGA GATTGTGCAC AAGCCTGGAC CTGTATCGCT TACTGTGGCC 
AAATGCTGGG ACCCAAACCC TAACAGTTGC TTTGCTTTGC CGAGGAGTGA ACCCATCCGG 
CCTATTGACC CTGCAGCCTG GGTGTCCCAT ACAGCAGCCA TGACGGGTGT CTATCCTCCC 
TACGGCATGA GTCCATCCAT GAGCACAGTC ACCTCCACCA GCTCCTCCAT TTCCAGCTCC 
ATCCCCGAGA CCGAGAGATT TGATGACTAC CACCTGTCCG TCCACAGCGA CATGGCAACA 
GTTGCAAAAG CAATGGCCTG TCCAGACTCG GGATTGGAAG TTCGAGACCG AATGTGGCTG 
AAGATCACTA TAGCCAATGC TTTCATAGGT TCGGATGTGG TGGACTGGCT TTTTCATCAT 
GTGGAGGGGT TCACAGATCG CCGTGAAGCA CGAAAGTATG CCAGCAACCT GTTGAAGGCT 
GGCTTCATCC GCCACACGGT CAACAAAATC ACCTTCTCAG AGCAGTGCTA CTACATCTTT 
GGAGATCTTT CTGGCAACAT GGCCCATCTC TCTCTGCAAG ACCACGACGG GTCAAGTGGA 
GCATCTGATC AGGACACTCT TGCTCCTTTG CTTCACCCTG CTGAAGCTCC CTGGCCCGCA 
GCGTTCCCGT ACCAGTACCC AGCAGCCCAT TCACACCTGG AGGAAGCTGG AGGAGGCAGC 
GCAGGCAGTC AGCAAAGTGA AGGGAGCAGC GGTTCAAACT GCAGCTGGAG CAGGACTGAG 
GGCAAAACCG CAGCCGGAGA CTTCAGGTTA GGAGGCGGAA GCGAAATGGG TGACGCTCAC 
GAGTTTGACT TCCGCCGAGA CCGAGCACCC AGCGAACGCT CGGGCGTGCC CCCCAGCGAG 
GGCAGCGTGC GGAGCTCTCG GTCTCACAGC TACAGCCTGA AGCAGGGGTC TGCACGAGGT 
GGCCCGGGGT CACCATCCGG CCGACATCTT GCCCACATTC CCCCTGAACT CACTGGCTCT 
CGCCGCTCCT GCAACACAGC CAACGGGGAG TTCTTCGTCG ACATCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutational analysis of dishevelled genes in zebrafish reveals distinct functions in embryonic patterning and gastrulation cell movements.
PLoS genetics14(8)e1007551(2018 08)
Xing YY,Cheng XN,Li YL,Zhang C,Saquet A,Liu YY,Shao M,Shi DL

CFTR mutation enhances Dishevelled degradation and results in impairment of Wnt-dependent hematopoiesis.
Cell death & disease9(3)275(2018 02)
Sun H,Wang Y,Zhang J,Chen Y,Liu Y,Lin Z,Liu M,Sheng K,Liao H,Tsang KS,Zhang X,Jiang X,Xu W,Mao M,Chan HC

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.
Scientific reports510673(2015 Jun)
Hsu CH,Lin JS,Po Lai K,Li JW,Chan TF,You MS,Tse WK,Jiang YJ

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dvl3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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