cd40lg cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cd40lg
Entrez Gene ID	793819
Full Name	CD40 ligand
General protein information	Preferred Names CD40 ligand Names CD40 ligand CD154 TNFSF5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144809.1	NP_001138281.1	CD40 ligand

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model.
Liu GP, Xiang LX, Shao T, Lin AF, Shao JZ
Developmental and comparative immunology84353-360(2018 07)

Essential Roles of TIM-1 and TIM-4 Homologs in Adaptive Humoral Immunity in a Zebrafish Model.
Xu XG, Hu JF, Ma JX, Nie L, Shao T, Xiang LX, Shao JZ
Journal of immunology (Baltimore, Md. : 1950)196(4)1686-99(2016 Feb)

Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Wan F, Hu CB, Ma JX, Gao K, Xiang LX, Shao JZ
Frontiers in immunology7675(2016)

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Biswas G, Kinoshita S, Kono T, Hikima J, Sakai M
Marine genomics2225-36(2015 Aug)

B cells in teleost fish act as pivotal initiating APCs in priming adaptive immunity: an evolutionary perspective on the origin of the B-1 cell subset and B7 molecules.
Zhu LY, Lin AF, Shao T, Nie L, Dong WR, Xiang LX, Shao JZ
Journal of immunology (Baltimore, Md. : 1950)192(6)2699-714(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cd40lg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cd40lg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30666	NM_001144809.1 Latest version!	Danio rerio CD40 ligand (cd40lg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cd40lg ( NM_001144809.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001138281.1 Danio rerio CD40 ligand (cd40lg), mRNA.

Price & Availability↑

CloneID	ODa30666
Clone ID Related Accession (Same CDS sequence)	NM_001144809.1
Accession Version	NM_001144809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1536940800000
Organism	Danio rerio(zebrafish)
Product	CD40 ligand
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ534590.1. On Jan 25, 2009 this sequence version replaced XM_001333619.1. ##Evidence-Data-START## Transcript exon combination :: FJ534590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATAAACA CCTTTCACAG TAGCTACAAC CCCCCACCGG TGCCTCCACG CGCAGGATAC 
AAGAAAGCCC AGCCATCGGG AAACACACCT TTAGTCAAGT TTTTGTCAGT GATGCTACTG 
CTGTTAATGA CACTGACCTT CGGAGGCTTC CTCTACCTGT TCCAGAAAGT CAACGGGGAA 
CTGCAATGGG TCTATCAGGA AGACACGGCG ACTCTGAAGA AACTACAGCA ATGTGCGAAT 
GGCAACAGGG CACAAGAATG TCAAATTCTG ATAGAAAAAG TGAATACTGT AATGGGAAAG 
GTTTCACTGG AAAATGGAAC ATTTTCTAAG TTTACCGGAG GACCTTCGAG GAACGGACCT 
GCTGCACACA TGGTTTTCCT GAGTGAACAC AATGAGAAAT ATAAGGAATC AGAGGCCTTG 
AGGTCAAACA GTCTTCTTTG GGACAAAGAA CGTTCACTGC TACAAGGCGT CCAGATTACC 
GTCAAAAGGG ACAGGCTAAC CATTCAAACT CCCGGAGTCT ACTTCATCTA TTCCCAAGTC 
ACCTTTTCCA GACGGGCCAC TAAAAATCCA CTAAAGCAAA CCATAAACCG ATCAGGACCA 
AAAAATGAGA ACAAGGAATT ACTCAAGTCT TTCTGCACCT TGCATGAGAA CACAGAAGAA 
ATGTGTACGG CATCATCAGC AGGAGTGTTT AGACTGGAGA AAGACCAACA AGTCTATGTT 
ACAGTAACAG ACAGATCCCT GGTAAACAAG AAGTACTGCT GGTTTGGATT ATTCAAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138281.1
CDS	256..1038
Translation	MINTFHSSYNPPPVPPRAGYKKAQPSGNTPLVKFLSVMLLLLMTLTFGGFLYLFQKVNGELQWVYQEDTATLKKLQQCANGNRAQECQILIEKVNTVMGKVSLENGTFSKFTGGPSRNGPAAHMVFLSEHNEKYKESEALRSNSLLWDKERSLLQGVQITVKRDRLTIQTPGVYFIYSQVTFSRRATKNPLKQTINRSGPKNENKELLKSFCTLHENTEEMCTASSAGVFRLEKDQQVYVTVTDRSLVNKKYCWFGLFKL

Target ORF information:

RefSeq Version	NM_001144809.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CD40 ligand (cd40lg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144809.1

ORF Insert Sequence:

ATGATAAACA CCTTTCACAG TAGCTACAAC CCCCCACCGG TGCCTCCACG CGCAGGATAC 
AAGAAAGCCC AGCCATCGGG AAACACACCT TTAGTCAAGT TTTTGTCAGT GATGCTACTG 
CTGTTAATGA CACTGACCTT CGGAGGCTTC CTCTACCTGT TCCAGAAAGT CAACGGGGAA 
CTGCAATGGG TCTATCAGGA AGACACGGCG ACTCTGAAGA AACTACAGCA ATGTGCGAAT 
GGCAACAGGG CACAAGAATG TCAAATTCTG ATAGAAAAAG TGAATACTGT AATGGGAAAG 
GTTTCACTGG AAAATGGAAC ATTTTCTAAG TTTACCGGAG GACCTTCGAG GAACGGACCT 
GCTGCACACA TGGTTTTCCT GAGTGAACAC AATGAGAAAT ATAAGGAATC AGAGGCCTTG 
AGGTCAAACA GTCTTCTTTG GGACAAAGAA CGTTCACTGC TACAAGGCGT CCAGATTACC 
GTCAAAAGGG ACAGGCTAAC CATTCAAACT CCCGGAGTCT ACTTCATCTA TTCCCAAGTC 
ACCTTTTCCA GACGGGCCAC TAAAAATCCA CTAAAGCAAA CCATAAACCG ATCAGGACCA 
AAAAATGAGA ACAAGGAATT ACTCAAGTCT TTCTGCACCT TGCATGAGAA CACAGAAGAA 
ATGTGTACGG CATCATCAGC AGGAGTGTTT AGACTGGAGA AAGACCAACA AGTCTATGTT 
ACAGTAACAG ACAGATCCCT GGTAAACAAG AAGTACTGCT GGTTTGGATT ATTCAAGCTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model.
Developmental and comparative immunology84353-360(2018 07)
Liu GP,Xiang LX,Shao T,Lin AF,Shao JZ

Essential Roles of TIM-1 and TIM-4 Homologs in Adaptive Humoral Immunity in a Zebrafish Model.
Journal of immunology (Baltimore, Md. : 1950)196(4)1686-99(2016 Feb)
Xu XG,Hu JF,Ma JX,Nie L,Shao T,Xiang LX,Shao JZ

Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Frontiers in immunology7675(2016)
Wan F,Hu CB,Ma JX,Gao K,Xiang LX,Shao JZ

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Marine genomics2225-36(2015 Aug)
Biswas G,Kinoshita S,Kono T,Hikima J,Sakai M

B cells in teleost fish act as pivotal initiating APCs in priming adaptive immunity: an evolutionary perspective on the origin of the B-1 cell subset and B7 molecules.
Journal of immunology (Baltimore, Md. : 1950)192(6)2699-714(2014 Mar)
Zhu LY,Lin AF,Shao T,Nie L,Dong WR,Xiang LX,Shao JZ