map4k6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	map4k6
Entrez Gene ID	692287
Full Name	mitogen-activated protein kinase kinase kinase kinase 6
Synonyms	zgc:136354
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase kinase 6 Names mitogen-activated protein kinase kinase kinase kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161421.3	XP_005161478.1	mitogen-activated protein kinase kinase kinase kinase 2 isoform X1
XM_021468905.1	XP_021324580.1	mitogen-activated protein kinase kinase kinase kinase 2 isoform X2
XM_005161421.4	XP_005161478.1	mitogen-activated protein kinase kinase kinase kinase 2 isoform X1
NM_001045260.2	NP_001038725.1	mitogen-activated protein kinase kinase kinase kinase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MAP4K2	XP_005631567.1
Homo sapiens (human)	MAP4K2	NP_004570.2
Pan troglodytes (chimpanzee)	MAP4K2	XP_001166072.1
Macaca mulatta (Rhesus monkey)	LOC699331	XP_001085106.2
Bos taurus (cattle)	MAP4K2	NP_001193204.1
Mus musculus (house mouse)	Map4k2	NP_033032.1
Rattus norvegicus (Norway rat)	Map4k2	NP_001099799.1
Danio rerio (zebrafish)	map4k2	XP_003198970.1
Danio rerio (zebrafish)	zgc:136354	NP_001038725.1
Danio rerio (zebrafish)	map4k2l	NP_001035448.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following map4k6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map4k6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02284	NM_001045260.2 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa150944	XM_021468905.1 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa26104	XM_005161421.4 Latest version!	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa26104	XM_005161421.3	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map4k6 ( NM_001045260.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038725.1 Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), mRNA.

Price & Availability↑

CloneID	ODa02284
Clone ID Related Accession (Same CDS sequence)	NM_001045260.2
Accession Version	NM_001045260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC116514.1. On Aug 31, 2012 this sequence version replaced NM_001045260.1. ##Evidence-Data-START## Transcript exon combination :: BC116514.1, BC165706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG ACCCAGGAGG AACAAGGACT 
TATTCCACCC CGCCGGACTC TCCTGTCTCT CCAGAATGGA GCACTCTCAG AAGGAAACAG 
GATGACTCTA AAGCTGTTGT CAATGGACTG CCACCTACAC CACAAGTACA TATGGGGGCC 
TGCTTCTCCA AAGTATTCAA CGGCTGTCCA TTAAAGACTC ACTGTGCTGT TACATGGGTT 
CTACCTAAAA CCAGAGATCA ATATCTTATG CTGGGTGCTG AGGAAGGGGT CTACACCCTC 
AACCTCAATG AATTACATGA GGACACTATG GAAAAGTTGC TTCCACAGAG ATGCATTTGG 
TTATATGTGA TGAACAATGT CCTTATGTCC ATCTCAGGAA AGTCATCACA GTTGACTTCT 
CATAGCCTGC CAGCACTGTT TGAGTATCGC AGAAATATGC AACGTAGACA GGGACATCTC 
GCAATTAATG CACACCGCCT GAGCACAAAG ATAAATGCAA GGAAGTATGC CATGTCAGTA 
AAAATCCCAG ACACCAAGGG CTGTAGAAGA TGCAGTGTGG TACGAAACCC CTACAATGAC 
AGTGTTTTCT TGTGTGCCGC TGTGCCCACT GGATTGGTTC TTCTCATGTG GTATGAACCT 
TTGCAGAAGT TCATGCAGCT TAAGCACATT GCTTTGAGTC TGCCAGAGTT CCTGCCTATC 
TTTGAACTAC TTGTACAGGA AACTAAGGAG CTGCCACAGG TCTGTGTCGG GGTGAGAAGT 
TATCCCAACC AAAAAGACAC CTCTGGAGAG ATGCACTTTG ACATCATCCA CCTGGATGAC 
ACACCACAGG GCCTGCCAGA TGGTGAGACT CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC 
AGGGACACGA TTCTTGTCGC ACTGGAAAAC ATCGTGAAGG TTGTTAATTT GCAGGCATGC 
ATGAGCCAAC AGAAGACCCC TGAACTAGAT TTTGAGTTTT CCATTCACAC GCTGGTGTGC 
CTGCAGGACA GTGTTCTTGT CTTCTGGAAG CATGGCCTAA AAGGAAAGAG TTTACAAACT 
AATGAGGTTA CCCAGGAGAT CACAGATAGA AGTCGAGTTT TTCAAGTTTT GGGAACATCT 
AGGGATATTA TTTTGCAAAG TACTCCTACA GATGATCCGT CGGCCATGAG CAACCTATAC 
ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038725.1
CDS	138..2807
Translation	MDTAGVSHTDPLDDYELIHRIGSGTYGDVFKARSIKTSVIAAIKVVKLDPGDDISSIQNEITMMKDCTHKNIVAYFGSYLRNNKLWICMEFCGGGSLQDIYHVTGPLKERQIAYVSRETLQGLHHLHQTGKMHRDIKGANILLTERGDVKLADFGVAAEINASVAKRKSFIGTPYWMAPEVAAVEKKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALMLMSKSSFQPPKLKEKNKWSTEFHSFIKMALTKNPRKRPTAEKILQHAFVMQLLTRNLVIELLDTVNNPDLQQTQTMDESDLETCSVLPDKIHSLAKLPVQRTLSEQQFDHVKFGLPMRKETDPCPVLEAYDDWSSISADKDSPSLLECVEEALMERSLTIKRVSSVEHSPNEEDKYGTVKRMASSPQSTALCHQEKKAPTPSSIPSPIIGSSSLKNPSPNTNPSLLSESALLIGSTLSHMSLITNSCLFTDSSFLSHSTSLGSLCLTPTEDPGGTRTYSTPPDSPVSPEWSTLRRKQDDSKAVVNGLPPTPQVHMGACFSKVFNGCPLKTHCAVTWVLPKTRDQYLMLGAEEGVYTLNLNELHEDTMEKLLPQRCIWLYVMNNVLMSISGKSSQLTSHSLPALFEYRRNMQRRQGHLAINAHRLSTKINARKYAMSVKIPDTKGCRRCSVVRNPYNDSVFLCAAVPTGLVLLMWYEPLQKFMQLKHIALSLPEFLPIFELLVQETKELPQVCVGVRSYPNQKDTSGEMHFDIIHLDDTPQGLPDGETLEVKQVIQLDRDTILVALENIVKVVNLQACMSQQKTPELDFEFSIHTLVCLQDSVLVFWKHGLKGKSLQTNEVTQEITDRSRVFQVLGTSRDIILQSTPTDDPSAMSNLYILTGHESSY

Target ORF information:

RefSeq Version	NM_001045260.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045260.2

ORF Insert Sequence:

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG ACCCAGGAGG AACAAGGACT 
TATTCCACCC CGCCGGACTC TCCTGTCTCT CCAGAATGGA GCACTCTCAG AAGGAAACAG 
GATGACTCTA AAGCTGTTGT CAATGGACTG CCACCTACAC CACAAGTACA TATGGGGGCC 
TGCTTCTCCA AAGTATTCAA CGGCTGTCCA TTAAAGACTC ACTGTGCTGT TACATGGGTT 
CTACCTAAAA CCAGAGATCA ATATCTTATG CTGGGTGCTG AGGAAGGGGT CTACACCCTC 
AACCTCAATG AATTACATGA GGACACTATG GAAAAGTTGC TTCCACAGAG ATGCATTTGG 
TTATATGTGA TGAACAATGT CCTTATGTCC ATCTCAGGAA AGTCATCACA GTTGACTTCT 
CATAGCCTGC CAGCACTGTT TGAGTATCGC AGAAATATGC AACGTAGACA GGGACATCTC 
GCAATTAATG CACACCGCCT GAGCACAAAG ATAAATGCAA GGAAGTATGC CATGTCAGTA 
AAAATCCCAG ACACCAAGGG CTGTAGAAGA TGCAGTGTGG TACGAAACCC CTACAATGAC 
AGTGTTTTCT TGTGTGCCGC TGTGCCCACT GGATTGGTTC TTCTCATGTG GTATGAACCT 
TTGCAGAAGT TCATGCAGCT TAAGCACATT GCTTTGAGTC TGCCAGAGTT CCTGCCTATC 
TTTGAACTAC TTGTACAGGA AACTAAGGAG CTGCCACAGG TCTGTGTCGG GGTGAGAAGT 
TATCCCAACC AAAAAGACAC CTCTGGAGAG ATGCACTTTG ACATCATCCA CCTGGATGAC 
ACACCACAGG GCCTGCCAGA TGGTGAGACT CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC 
AGGGACACGA TTCTTGTCGC ACTGGAAAAC ATCGTGAAGG TTGTTAATTT GCAGGCATGC 
ATGAGCCAAC AGAAGACCCC TGAACTAGAT TTTGAGTTTT CCATTCACAC GCTGGTGTGC 
CTGCAGGACA GTGTTCTTGT CTTCTGGAAG CATGGCCTAA AAGGAAAGAG TTTACAAACT 
AATGAGGTTA CCCAGGAGAT CACAGATAGA AGTCGAGTTT TTCAAGTTTT GGGAACATCT 
AGGGATATTA TTTTGCAAAG TACTCCTACA GATGATCCGT CGGCCATGAG CAACCTATAC 
ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map4k6 ( XM_021468905.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324580.1 Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150944
Clone ID Related Accession (Same CDS sequence)	XM_021468905.1
Accession Version	XM_021468905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTAA GGCCCGTAGC ATCAAGACTT CAGTCATTGC AGCCATTAAA GTGGTCAAAC 
TGGACCCTGG GGCTACACCA CCTCCATCAG ACTGGAAAAA TGCATAGAGA CATAAAAGGT 
GCCAACATTC TTTTGACAGA GCGAGGAGAT GTCAAACTAG CGGATTTTGG AGTGGCAGCT 
GAGATCAACG CATCTGTTGC TAAAAGAAAA TCCTTTATCG GGACACCATA CTGGATGGCT 
CCAGAAGTTG CAGCCGTGGA AAAGAAAGGT GGTTACAACC AGTTGTGTGA TATCTGGGCA 
GTGGGCATCA CAGCTATTGA GCTAGCAGAG CTACAGCCAC CTATGTTTGA CCTACACCCT 
ATGAGAGCTT TAATGCTGAT GTCCAAGAGC AGTTTTCAGC CTCCGAAACT GAAGGAAAAG 
AACAAATGGT CCACAGAATT TCACAGTTTC ATCAAAATGG CTCTCACCAA GAACCCTCGG 
AAAAGGCCAA CAGCAGAAAA GATCCTACAA CATGCTTTTG TCATGCAGCT CCTCACACGC 
AACCTTGTTA TAGAGCTGCT GGACACAGTC AATAACCCTG ATCTGCAACA GACACAGACT 
ATGGATGAAA GCGATCTAGA GACTTGCAGC GTCCTACCAG ATAAGATTCA TTCTTTAGCA 
AAACTTCCTG TTCAGAGGAC TCTGTCAGAA CAGCAGTTTG ACCATGTGAA GTTTGGACTA 
CCTATGAGAA AGGAGACAGA TCCCTGTCCA GTTCTGGAGG CATATGATGA CTGGAGCTCT 
ATCAGCGCAG ACAAGGACTC ACCGAGTTTG TTAGAATGTG TGGAAGAGGC CTTGATGGAA 
AGGAGTTTAA CTATAAAGAG GGTCTCTTCA GTTGAGCACT CACCAAATGA AGAGGATAAA 
TATGGGACTG TGAAGAGGAT GGCCTCTTCT CCCCAATCAA CAGCCCTCTG CCATCAAGAA 
AAAAAAGCTC CCACTCCCTC TTCTATCCCC TCGCCTATTA TTGGCTCTAG CTCTCTGAAG 
AACCCTTCCC CCAACACTAA CCCTAGCCTA CTCAGTGAAT CGGCTTTGCT TATTGGTTCA 
ACATTGTCCC ACATGTCGCT CATCACAAAC TCCTGCCTAT TCACAGACTC CTCCTTCCTC 
AGTCATTCCA CCAGTCTAGG TTCCCTCTGT TTAACTCCAA CTGAAGGTTT AATACCAGAG 
AGCTCTGTCG CTCCAGACCC AGGAGGAACA AGGACTTATT CCACCCCGCC GGACTCTCCT 
GTCTCTCCAG AATGGAGCAC TCTCAGAAGG AAACAGGATG ACTCTAAAGC TGTTGTCAAT 
GGACTGCCAC CTACACCACA AGTACATATG GGGGCCTGCT TCTCCAAAGT ATTCAACGGC 
TGTCCATTAA AGACTCACTG TGCTGTTACA TGGGTTCTAC CTAAAACCAG AGATCAATAT 
CTTATGCTGG GTGCTGAGGA AGGGGTCTAC ACCCTCAACC TCAATGAATT ACATGAGGAC 
ACTATGGAAA AGTTGCTTCC ACAGAGATGC ATTTGGTTAT ATGTGATGAA CAATGTCCTT 
ATGTCCATCT CAGGAAAGTC ATCACAGTTG ACTTCTCATA GCCTGCCAGC ACTGTTTGAG 
TATCGCAGAA ATATGCAACG TAGACAGGGA CATCTCGCAA TTAATGCACA CCGCCTGAGC 
ACAAAGATAA ATGCAAGGAA GTATGCCATG TCAGTAAAAA TCCCAGACAC CAAGGGCTGT 
AGAAGATGCA GTGTGGTACG AAACCCCTAC AATGACAGTG TTTTCTTGTG TGCCGCTGTG 
CCCACTGGAT TGGTTCTTCT CATGTGGTAT GAACCTTTGC AGAAGTTCAT GCAGCTTAAG 
CACATTGCTT TGAGTCTGCC AGAGTTCCTG CCTATCTTTG AACTACTTGT ACAGGAAACT 
AAGGAGCTGC CACAGGTCTG TGTCGGGGTG AGAAGTTATC CCAACCAAAA AGACACCTCT 
GGAGAGATGC ACTTTGACAT CATCCACCTG GATGACACAC CACAGGGCCT GCCAGATGGT 
GAGACTCTGG AGGTCAAGCA AGTGATTCAG CTTGACAGGG ACACGATTCT TGTCGCACTG 
GAAAACATCG TGAAGGTTGT TAATTTGCAG GCATGCATGA GCCAACAGAA GACCCCTGAA 
CTAGATTTTG AGTTTTCCAT TCACACGCTG GTGTGCCTGC AGGACAGTGT TCTTGTCTTC 
TGGAAGCATG GCCTAAAAGG AAAGAGTTTA CAAACTAATG AGGTTACCCA GGAGATCACA 
GATAGAAGTC GAGTTTTTCA AGTTTTGGGA ACATCTAGGG ATATTATTTT GCAAAGTACT 
CCTACAGATG ATCCGTCGGC CATGAGCAAC CTATACATAC TCACAGGGCA CGAAAGCAGC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324580.1
CDS	133..2538
Translation	MFLRPVASRLQSLQPLKWSNWTLGLHHLHQTGKMHRDIKGANILLTERGDVKLADFGVAAEINASVAKRKSFIGTPYWMAPEVAAVEKKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALMLMSKSSFQPPKLKEKNKWSTEFHSFIKMALTKNPRKRPTAEKILQHAFVMQLLTRNLVIELLDTVNNPDLQQTQTMDESDLETCSVLPDKIHSLAKLPVQRTLSEQQFDHVKFGLPMRKETDPCPVLEAYDDWSSISADKDSPSLLECVEEALMERSLTIKRVSSVEHSPNEEDKYGTVKRMASSPQSTALCHQEKKAPTPSSIPSPIIGSSSLKNPSPNTNPSLLSESALLIGSTLSHMSLITNSCLFTDSSFLSHSTSLGSLCLTPTEGLIPESSVAPDPGGTRTYSTPPDSPVSPEWSTLRRKQDDSKAVVNGLPPTPQVHMGACFSKVFNGCPLKTHCAVTWVLPKTRDQYLMLGAEEGVYTLNLNELHEDTMEKLLPQRCIWLYVMNNVLMSISGKSSQLTSHSLPALFEYRRNMQRRQGHLAINAHRLSTKINARKYAMSVKIPDTKGCRRCSVVRNPYNDSVFLCAAVPTGLVLLMWYEPLQKFMQLKHIALSLPEFLPIFELLVQETKELPQVCVGVRSYPNQKDTSGEMHFDIIHLDDTPQGLPDGETLEVKQVIQLDRDTILVALENIVKVVNLQACMSQQKTPELDFEFSIHTLVCLQDSVLVFWKHGLKGKSLQTNEVTQEITDRSRVFQVLGTSRDIILQSTPTDDPSAMSNLYILTGHESSY

Target ORF information:

RefSeq Version	XM_021468905.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468905.1

ORF Insert Sequence:

ATGTTTTTAA GGCCCGTAGC ATCAAGACTT CAGTCATTGC AGCCATTAAA GTGGTCAAAC 
TGGACCCTGG GGCTACACCA CCTCCATCAG ACTGGAAAAA TGCATAGAGA CATAAAAGGT 
GCCAACATTC TTTTGACAGA GCGAGGAGAT GTCAAACTAG CGGATTTTGG AGTGGCAGCT 
GAGATCAACG CATCTGTTGC TAAAAGAAAA TCCTTTATCG GGACACCATA CTGGATGGCT 
CCAGAAGTTG CAGCCGTGGA AAAGAAAGGT GGTTACAACC AGTTGTGTGA TATCTGGGCA 
GTGGGCATCA CAGCTATTGA GCTAGCAGAG CTACAGCCAC CTATGTTTGA CCTACACCCT 
ATGAGAGCTT TAATGCTGAT GTCCAAGAGC AGTTTTCAGC CTCCGAAACT GAAGGAAAAG 
AACAAATGGT CCACAGAATT TCACAGTTTC ATCAAAATGG CTCTCACCAA GAACCCTCGG 
AAAAGGCCAA CAGCAGAAAA GATCCTACAA CATGCTTTTG TCATGCAGCT CCTCACACGC 
AACCTTGTTA TAGAGCTGCT GGACACAGTC AATAACCCTG ATCTGCAACA GACACAGACT 
ATGGATGAAA GCGATCTAGA GACTTGCAGC GTCCTACCAG ATAAGATTCA TTCTTTAGCA 
AAACTTCCTG TTCAGAGGAC TCTGTCAGAA CAGCAGTTTG ACCATGTGAA GTTTGGACTA 
CCTATGAGAA AGGAGACAGA TCCCTGTCCA GTTCTGGAGG CATATGATGA CTGGAGCTCT 
ATCAGCGCAG ACAAGGACTC ACCGAGTTTG TTAGAATGTG TGGAAGAGGC CTTGATGGAA 
AGGAGTTTAA CTATAAAGAG GGTCTCTTCA GTTGAGCACT CACCAAATGA AGAGGATAAA 
TATGGGACTG TGAAGAGGAT GGCCTCTTCT CCCCAATCAA CAGCCCTCTG CCATCAAGAA 
AAAAAAGCTC CCACTCCCTC TTCTATCCCC TCGCCTATTA TTGGCTCTAG CTCTCTGAAG 
AACCCTTCCC CCAACACTAA CCCTAGCCTA CTCAGTGAAT CGGCTTTGCT TATTGGTTCA 
ACATTGTCCC ACATGTCGCT CATCACAAAC TCCTGCCTAT TCACAGACTC CTCCTTCCTC 
AGTCATTCCA CCAGTCTAGG TTCCCTCTGT TTAACTCCAA CTGAAGGTTT AATACCAGAG 
AGCTCTGTCG CTCCAGACCC AGGAGGAACA AGGACTTATT CCACCCCGCC GGACTCTCCT 
GTCTCTCCAG AATGGAGCAC TCTCAGAAGG AAACAGGATG ACTCTAAAGC TGTTGTCAAT 
GGACTGCCAC CTACACCACA AGTACATATG GGGGCCTGCT TCTCCAAAGT ATTCAACGGC 
TGTCCATTAA AGACTCACTG TGCTGTTACA TGGGTTCTAC CTAAAACCAG AGATCAATAT 
CTTATGCTGG GTGCTGAGGA AGGGGTCTAC ACCCTCAACC TCAATGAATT ACATGAGGAC 
ACTATGGAAA AGTTGCTTCC ACAGAGATGC ATTTGGTTAT ATGTGATGAA CAATGTCCTT 
ATGTCCATCT CAGGAAAGTC ATCACAGTTG ACTTCTCATA GCCTGCCAGC ACTGTTTGAG 
TATCGCAGAA ATATGCAACG TAGACAGGGA CATCTCGCAA TTAATGCACA CCGCCTGAGC 
ACAAAGATAA ATGCAAGGAA GTATGCCATG TCAGTAAAAA TCCCAGACAC CAAGGGCTGT 
AGAAGATGCA GTGTGGTACG AAACCCCTAC AATGACAGTG TTTTCTTGTG TGCCGCTGTG 
CCCACTGGAT TGGTTCTTCT CATGTGGTAT GAACCTTTGC AGAAGTTCAT GCAGCTTAAG 
CACATTGCTT TGAGTCTGCC AGAGTTCCTG CCTATCTTTG AACTACTTGT ACAGGAAACT 
AAGGAGCTGC CACAGGTCTG TGTCGGGGTG AGAAGTTATC CCAACCAAAA AGACACCTCT 
GGAGAGATGC ACTTTGACAT CATCCACCTG GATGACACAC CACAGGGCCT GCCAGATGGT 
GAGACTCTGG AGGTCAAGCA AGTGATTCAG CTTGACAGGG ACACGATTCT TGTCGCACTG 
GAAAACATCG TGAAGGTTGT TAATTTGCAG GCATGCATGA GCCAACAGAA GACCCCTGAA 
CTAGATTTTG AGTTTTCCAT TCACACGCTG GTGTGCCTGC AGGACAGTGT TCTTGTCTTC 
TGGAAGCATG GCCTAAAAGG AAAGAGTTTA CAAACTAATG AGGTTACCCA GGAGATCACA 
GATAGAAGTC GAGTTTTTCA AGTTTTGGGA ACATCTAGGG ATATTATTTT GCAAAGTACT 
CCTACAGATG ATCCGTCGGC CATGAGCAAC CTATACATAC TCACAGGGCA CGAAAGCAGC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map4k6 ( XM_005161421.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161478.1 Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26104
Clone ID Related Accession (Same CDS sequence)	XM_005161421.3 , XM_005161421.4
Accession Version	XM_005161421.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	mitogen-activated protein kinase kinase kinase kinase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG GTTTAATACC AGAGAGCTCT 
GTCGCTCCAG ACCCAGGAGG AACAAGGACT TATTCCACCC CGCCGGACTC TCCTGTCTCT 
CCAGAATGGA GCACTCTCAG AAGGAAACAG GATGACTCTA AAGCTGTTGT CAATGGACTG 
CCACCTACAC CACAAGTACA TATGGGGGCC TGCTTCTCCA AAGTATTCAA CGGCTGTCCA 
TTAAAGACTC ACTGTGCTGT TACATGGGTT CTACCTAAAA CCAGAGATCA ATATCTTATG 
CTGGGTGCTG AGGAAGGGGT CTACACCCTC AACCTCAATG AATTACATGA GGACACTATG 
GAAAAGTTGC TTCCACAGAG ATGCATTTGG TTATATGTGA TGAACAATGT CCTTATGTCC 
ATCTCAGGAA AGTCATCACA GTTGACTTCT CATAGCCTGC CAGCACTGTT TGAGTATCGC 
AGAAATATGC AACGTAGACA GGGACATCTC GCAATTAATG CACACCGCCT GAGCACAAAG 
ATAAATGCAA GGAAGTATGC CATGTCAGTA AAAATCCCAG ACACCAAGGG CTGTAGAAGA 
TGCAGTGTGG TACGAAACCC CTACAATGAC AGTGTTTTCT TGTGTGCCGC TGTGCCCACT 
GGATTGGTTC TTCTCATGTG GTATGAACCT TTGCAGAAGT TCATGCAGCT TAAGCACATT 
GCTTTGAGTC TGCCAGAGTT CCTGCCTATC TTTGAACTAC TTGTACAGGA AACTAAGGAG 
CTGCCACAGG TCTGTGTCGG GGTGAGAAGT TATCCCAACC AAAAAGACAC CTCTGGAGAG 
ATGCACTTTG ACATCATCCA CCTGGATGAC ACACCACAGG GCCTGCCAGA TGGTGAGACT 
CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC AGGGACACGA TTCTTGTCGC ACTGGAAAAC 
ATCGTGAAGG TTGTTAATTT GCAGGCATGC ATGAGCCAAC AGAAGACCCC TGAACTAGAT 
TTTGAGTTTT CCATTCACAC GCTGGTGTGC CTGCAGGACA GTGTTCTTGT CTTCTGGAAG 
CATGGCCTAA AAGGAAAGAG TTTACAAACT AATGAGGTTA CCCAGGAGAT CACAGATAGA 
AGTCGAGTTT TTCAAGTTTT GGGAACATCT AGGGATATTA TTTTGCAAAG TACTCCTACA 
GATGATCCGT CGGCCATGAG CAACCTATAC ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161478.1
CDS	194..2893
Translation	MDTAGVSHTDPLDDYELIHRIGSGTYGDVFKARSIKTSVIAAIKVVKLDPGDDISSIQNEITMMKDCTHKNIVAYFGSYLRNNKLWICMEFCGGGSLQDIYHVTGPLKERQIAYVSRETLQGLHHLHQTGKMHRDIKGANILLTERGDVKLADFGVAAEINASVAKRKSFIGTPYWMAPEVAAVEKKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALMLMSKSSFQPPKLKEKNKWSTEFHSFIKMALTKNPRKRPTAEKILQHAFVMQLLTRNLVIELLDTVNNPDLQQTQTMDESDLETCSVLPDKIHSLAKLPVQRTLSEQQFDHVKFGLPMRKETDPCPVLEAYDDWSSISADKDSPSLLECVEEALMERSLTIKRVSSVEHSPNEEDKYGTVKRMASSPQSTALCHQEKKAPTPSSIPSPIIGSSSLKNPSPNTNPSLLSESALLIGSTLSHMSLITNSCLFTDSSFLSHSTSLGSLCLTPTEGLIPESSVAPDPGGTRTYSTPPDSPVSPEWSTLRRKQDDSKAVVNGLPPTPQVHMGACFSKVFNGCPLKTHCAVTWVLPKTRDQYLMLGAEEGVYTLNLNELHEDTMEKLLPQRCIWLYVMNNVLMSISGKSSQLTSHSLPALFEYRRNMQRRQGHLAINAHRLSTKINARKYAMSVKIPDTKGCRRCSVVRNPYNDSVFLCAAVPTGLVLLMWYEPLQKFMQLKHIALSLPEFLPIFELLVQETKELPQVCVGVRSYPNQKDTSGEMHFDIIHLDDTPQGLPDGETLEVKQVIQLDRDTILVALENIVKVVNLQACMSQQKTPELDFEFSIHTLVCLQDSVLVFWKHGLKGKSLQTNEVTQEITDRSRVFQVLGTSRDIILQSTPTDDPSAMSNLYILTGHESSY

Target ORF information:

RefSeq Version	XM_005161421.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161421.3

ORF Insert Sequence:

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG GTTTAATACC AGAGAGCTCT 
GTCGCTCCAG ACCCAGGAGG AACAAGGACT TATTCCACCC CGCCGGACTC TCCTGTCTCT 
CCAGAATGGA GCACTCTCAG AAGGAAACAG GATGACTCTA AAGCTGTTGT CAATGGACTG 
CCACCTACAC CACAAGTACA TATGGGGGCC TGCTTCTCCA AAGTATTCAA CGGCTGTCCA 
TTAAAGACTC ACTGTGCTGT TACATGGGTT CTACCTAAAA CCAGAGATCA ATATCTTATG 
CTGGGTGCTG AGGAAGGGGT CTACACCCTC AACCTCAATG AATTACATGA GGACACTATG 
GAAAAGTTGC TTCCACAGAG ATGCATTTGG TTATATGTGA TGAACAATGT CCTTATGTCC 
ATCTCAGGAA AGTCATCACA GTTGACTTCT CATAGCCTGC CAGCACTGTT TGAGTATCGC 
AGAAATATGC AACGTAGACA GGGACATCTC GCAATTAATG CACACCGCCT GAGCACAAAG 
ATAAATGCAA GGAAGTATGC CATGTCAGTA AAAATCCCAG ACACCAAGGG CTGTAGAAGA 
TGCAGTGTGG TACGAAACCC CTACAATGAC AGTGTTTTCT TGTGTGCCGC TGTGCCCACT 
GGATTGGTTC TTCTCATGTG GTATGAACCT TTGCAGAAGT TCATGCAGCT TAAGCACATT 
GCTTTGAGTC TGCCAGAGTT CCTGCCTATC TTTGAACTAC TTGTACAGGA AACTAAGGAG 
CTGCCACAGG TCTGTGTCGG GGTGAGAAGT TATCCCAACC AAAAAGACAC CTCTGGAGAG 
ATGCACTTTG ACATCATCCA CCTGGATGAC ACACCACAGG GCCTGCCAGA TGGTGAGACT 
CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC AGGGACACGA TTCTTGTCGC ACTGGAAAAC 
ATCGTGAAGG TTGTTAATTT GCAGGCATGC ATGAGCCAAC AGAAGACCCC TGAACTAGAT 
TTTGAGTTTT CCATTCACAC GCTGGTGTGC CTGCAGGACA GTGTTCTTGT CTTCTGGAAG 
CATGGCCTAA AAGGAAAGAG TTTACAAACT AATGAGGTTA CCCAGGAGAT CACAGATAGA 
AGTCGAGTTT TTCAAGTTTT GGGAACATCT AGGGATATTA TTTTGCAAAG TACTCCTACA 
GATGATCCGT CGGCCATGAG CAACCTATAC ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map4k6 ( XM_005161421.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161478.1 Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26104
Clone ID Related Accession (Same CDS sequence)	XM_005161421.3 , XM_005161421.4
Accession Version	XM_005161421.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	mitogen-activated protein kinase kinase kinase kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161421.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG GTTTAATACC AGAGAGCTCT 
GTCGCTCCAG ACCCAGGAGG AACAAGGACT TATTCCACCC CGCCGGACTC TCCTGTCTCT 
CCAGAATGGA GCACTCTCAG AAGGAAACAG GATGACTCTA AAGCTGTTGT CAATGGACTG 
CCACCTACAC CACAAGTACA TATGGGGGCC TGCTTCTCCA AAGTATTCAA CGGCTGTCCA 
TTAAAGACTC ACTGTGCTGT TACATGGGTT CTACCTAAAA CCAGAGATCA ATATCTTATG 
CTGGGTGCTG AGGAAGGGGT CTACACCCTC AACCTCAATG AATTACATGA GGACACTATG 
GAAAAGTTGC TTCCACAGAG ATGCATTTGG TTATATGTGA TGAACAATGT CCTTATGTCC 
ATCTCAGGAA AGTCATCACA GTTGACTTCT CATAGCCTGC CAGCACTGTT TGAGTATCGC 
AGAAATATGC AACGTAGACA GGGACATCTC GCAATTAATG CACACCGCCT GAGCACAAAG 
ATAAATGCAA GGAAGTATGC CATGTCAGTA AAAATCCCAG ACACCAAGGG CTGTAGAAGA 
TGCAGTGTGG TACGAAACCC CTACAATGAC AGTGTTTTCT TGTGTGCCGC TGTGCCCACT 
GGATTGGTTC TTCTCATGTG GTATGAACCT TTGCAGAAGT TCATGCAGCT TAAGCACATT 
GCTTTGAGTC TGCCAGAGTT CCTGCCTATC TTTGAACTAC TTGTACAGGA AACTAAGGAG 
CTGCCACAGG TCTGTGTCGG GGTGAGAAGT TATCCCAACC AAAAAGACAC CTCTGGAGAG 
ATGCACTTTG ACATCATCCA CCTGGATGAC ACACCACAGG GCCTGCCAGA TGGTGAGACT 
CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC AGGGACACGA TTCTTGTCGC ACTGGAAAAC 
ATCGTGAAGG TTGTTAATTT GCAGGCATGC ATGAGCCAAC AGAAGACCCC TGAACTAGAT 
TTTGAGTTTT CCATTCACAC GCTGGTGTGC CTGCAGGACA GTGTTCTTGT CTTCTGGAAG 
CATGGCCTAA AAGGAAAGAG TTTACAAACT AATGAGGTTA CCCAGGAGAT CACAGATAGA 
AGTCGAGTTT TTCAAGTTTT GGGAACATCT AGGGATATTA TTTTGCAAAG TACTCCTACA 
GATGATCCGT CGGCCATGAG CAACCTATAC ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161478.1
CDS	194..2893
Translation	MDTAGVSHTDPLDDYELIHRIGSGTYGDVFKARSIKTSVIAAIKVVKLDPGDDISSIQNEITMMKDCTHKNIVAYFGSYLRNNKLWICMEFCGGGSLQDIYHVTGPLKERQIAYVSRETLQGLHHLHQTGKMHRDIKGANILLTERGDVKLADFGVAAEINASVAKRKSFIGTPYWMAPEVAAVEKKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALMLMSKSSFQPPKLKEKNKWSTEFHSFIKMALTKNPRKRPTAEKILQHAFVMQLLTRNLVIELLDTVNNPDLQQTQTMDESDLETCSVLPDKIHSLAKLPVQRTLSEQQFDHVKFGLPMRKETDPCPVLEAYDDWSSISADKDSPSLLECVEEALMERSLTIKRVSSVEHSPNEEDKYGTVKRMASSPQSTALCHQEKKAPTPSSIPSPIIGSSSLKNPSPNTNPSLLSESALLIGSTLSHMSLITNSCLFTDSSFLSHSTSLGSLCLTPTEGLIPESSVAPDPGGTRTYSTPPDSPVSPEWSTLRRKQDDSKAVVNGLPPTPQVHMGACFSKVFNGCPLKTHCAVTWVLPKTRDQYLMLGAEEGVYTLNLNELHEDTMEKLLPQRCIWLYVMNNVLMSISGKSSQLTSHSLPALFEYRRNMQRRQGHLAINAHRLSTKINARKYAMSVKIPDTKGCRRCSVVRNPYNDSVFLCAAVPTGLVLLMWYEPLQKFMQLKHIALSLPEFLPIFELLVQETKELPQVCVGVRSYPNQKDTSGEMHFDIIHLDDTPQGLPDGETLEVKQVIQLDRDTILVALENIVKVVNLQACMSQQKTPELDFEFSIHTLVCLQDSVLVFWKHGLKGKSLQTNEVTQEITDRSRVFQVLGTSRDIILQSTPTDDPSAMSNLYILTGHESSY

Target ORF information:

RefSeq Version	XM_005161421.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitogen-activated protein kinase kinase kinase kinase 6 (map4k6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161421.4

ORF Insert Sequence:

ATGGACACAG CAGGCGTATC GCACACGGAT CCGCTCGATG ACTATGAGCT AATACATCGC 
ATAGGCAGCG GGACATACGG AGATGTTTTT AAGGCCCGTA GCATCAAGAC TTCAGTCATT 
GCAGCCATTA AAGTGGTCAA ACTGGACCCT GGTGATGATA TTTCAAGCAT TCAAAATGAG 
ATAACCATGA TGAAAGACTG CACTCACAAA AACATTGTTG CCTATTTTGG CAGCTACCTA 
AGAAATAACA AGTTATGGAT CTGCATGGAA TTCTGTGGAG GAGGGTCATT GCAAGATATT 
TACCATGTCA CTGGTCCTCT GAAAGAGAGA CAGATAGCTT ATGTTAGCAG AGAGACACTT 
CAGGGGCTAC ACCACCTCCA TCAGACTGGA AAAATGCATA GAGACATAAA AGGTGCCAAC 
ATTCTTTTGA CAGAGCGAGG AGATGTCAAA CTAGCGGATT TTGGAGTGGC AGCTGAGATC 
AACGCATCTG TTGCTAAAAG AAAATCCTTT ATCGGGACAC CATACTGGAT GGCTCCAGAA 
GTTGCAGCCG TGGAAAAGAA AGGTGGTTAC AACCAGTTGT GTGATATCTG GGCAGTGGGC 
ATCACAGCTA TTGAGCTAGC AGAGCTACAG CCACCTATGT TTGACCTACA CCCTATGAGA 
GCTTTAATGC TGATGTCCAA GAGCAGTTTT CAGCCTCCGA AACTGAAGGA AAAGAACAAA 
TGGTCCACAG AATTTCACAG TTTCATCAAA ATGGCTCTCA CCAAGAACCC TCGGAAAAGG 
CCAACAGCAG AAAAGATCCT ACAACATGCT TTTGTCATGC AGCTCCTCAC ACGCAACCTT 
GTTATAGAGC TGCTGGACAC AGTCAATAAC CCTGATCTGC AACAGACACA GACTATGGAT 
GAAAGCGATC TAGAGACTTG CAGCGTCCTA CCAGATAAGA TTCATTCTTT AGCAAAACTT 
CCTGTTCAGA GGACTCTGTC AGAACAGCAG TTTGACCATG TGAAGTTTGG ACTACCTATG 
AGAAAGGAGA CAGATCCCTG TCCAGTTCTG GAGGCATATG ATGACTGGAG CTCTATCAGC 
GCAGACAAGG ACTCACCGAG TTTGTTAGAA TGTGTGGAAG AGGCCTTGAT GGAAAGGAGT 
TTAACTATAA AGAGGGTCTC TTCAGTTGAG CACTCACCAA ATGAAGAGGA TAAATATGGG 
ACTGTGAAGA GGATGGCCTC TTCTCCCCAA TCAACAGCCC TCTGCCATCA AGAAAAAAAA 
GCTCCCACTC CCTCTTCTAT CCCCTCGCCT ATTATTGGCT CTAGCTCTCT GAAGAACCCT 
TCCCCCAACA CTAACCCTAG CCTACTCAGT GAATCGGCTT TGCTTATTGG TTCAACATTG 
TCCCACATGT CGCTCATCAC AAACTCCTGC CTATTCACAG ACTCCTCCTT CCTCAGTCAT 
TCCACCAGTC TAGGTTCCCT CTGTTTAACT CCAACTGAAG GTTTAATACC AGAGAGCTCT 
GTCGCTCCAG ACCCAGGAGG AACAAGGACT TATTCCACCC CGCCGGACTC TCCTGTCTCT 
CCAGAATGGA GCACTCTCAG AAGGAAACAG GATGACTCTA AAGCTGTTGT CAATGGACTG 
CCACCTACAC CACAAGTACA TATGGGGGCC TGCTTCTCCA AAGTATTCAA CGGCTGTCCA 
TTAAAGACTC ACTGTGCTGT TACATGGGTT CTACCTAAAA CCAGAGATCA ATATCTTATG 
CTGGGTGCTG AGGAAGGGGT CTACACCCTC AACCTCAATG AATTACATGA GGACACTATG 
GAAAAGTTGC TTCCACAGAG ATGCATTTGG TTATATGTGA TGAACAATGT CCTTATGTCC 
ATCTCAGGAA AGTCATCACA GTTGACTTCT CATAGCCTGC CAGCACTGTT TGAGTATCGC 
AGAAATATGC AACGTAGACA GGGACATCTC GCAATTAATG CACACCGCCT GAGCACAAAG 
ATAAATGCAA GGAAGTATGC CATGTCAGTA AAAATCCCAG ACACCAAGGG CTGTAGAAGA 
TGCAGTGTGG TACGAAACCC CTACAATGAC AGTGTTTTCT TGTGTGCCGC TGTGCCCACT 
GGATTGGTTC TTCTCATGTG GTATGAACCT TTGCAGAAGT TCATGCAGCT TAAGCACATT 
GCTTTGAGTC TGCCAGAGTT CCTGCCTATC TTTGAACTAC TTGTACAGGA AACTAAGGAG 
CTGCCACAGG TCTGTGTCGG GGTGAGAAGT TATCCCAACC AAAAAGACAC CTCTGGAGAG 
ATGCACTTTG ACATCATCCA CCTGGATGAC ACACCACAGG GCCTGCCAGA TGGTGAGACT 
CTGGAGGTCA AGCAAGTGAT TCAGCTTGAC AGGGACACGA TTCTTGTCGC ACTGGAAAAC 
ATCGTGAAGG TTGTTAATTT GCAGGCATGC ATGAGCCAAC AGAAGACCCC TGAACTAGAT 
TTTGAGTTTT CCATTCACAC GCTGGTGTGC CTGCAGGACA GTGTTCTTGT CTTCTGGAAG 
CATGGCCTAA AAGGAAAGAG TTTACAAACT AATGAGGTTA CCCAGGAGAT CACAGATAGA 
AGTCGAGTTT TTCAAGTTTT GGGAACATCT AGGGATATTA TTTTGCAAAG TACTCCTACA 
GATGATCCGT CGGCCATGAG CAACCTATAC ATACTCACAG GGCACGAAAG CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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map4k6 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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