NIPBLA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIPBLA
Entrez Gene ID	570900
Full Name	nipped-B homolog a (Drosophila)
Synonyms	si:ch211-234b6.2,si:ch73-311f13.1
General protein information	Preferred Names nipped-B homolog a (Drosophila) Names nipped-B-like protein A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301188.3	XP_009299463.1	nipped-B-like protein A isoform X1
NM_001246284.1	NP_001233213.1	nipped-B-like protein A
XM_009301188.2	XP_009299463.1	nipped-B-like protein A isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

L-leucine partially rescues translational and developmental defects associated with zebrafish models of Cornelia de Lange syndrome.
Xu B, Sowa N, Cardenas ME, Gerton JL
Human molecular genetics24(6)1540-55(2015 Mar)

Nipbl and mediator cooperatively regulate gene expression to control limb development.
Muto A, Ikeda S, Lopez-Burks ME, Kikuchi Y, Calof AL, Lander AD, Schilling TF
PLoS genetics10(9)e1004671(2014 Sep)

From zebrafish heart jogging genes to mouse and human orthologs: using Gene Ontology to investigate mammalian heart development.
Khodiyar VK, Howe D, Talmud PJ, Breckenridge R, Lovering RC
F1000Research2242(2013)

Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome.
Muto A, Calof AL, Lander AD, Schilling TF
PLoS biology9(10)e1001181(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NIPBLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03093	NM_001246284.1 Latest version!	Danio rerio NIPBL cohesin loading factor a (nipbla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa46051	XM_009301188.3 Latest version!	Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa46051	XM_009301188.2	Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIPBLA ( NM_001246284.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001233213.1 Danio rerio NIPBL cohesin loading factor a (nipbla), mRNA.

Price & Availability↑

CloneID	ODa03093
Clone ID Related Accession (Same CDS sequence)	NM_001246284.1
Accession Version	NM_001246284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Danio rerio(zebrafish)
Product	nipped-B-like protein A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB630366.1. On Oct 10, 2011 this sequence version replaced XM_001920168.3. ##Evidence-Data-START## Transcript exon combination :: AB630366.1 [ECO:0000332] ##Evidence-Data-END##

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTGCCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCCAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCACGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGAAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTGAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATC 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCGGA GGATGATCAC AAGAAGGTTG ACGATGATGT TTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCTGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCACT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCTGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTCAGG 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGTGTGAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAAGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATCGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC AAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ACATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAGC AGCAGCAGAA CGGCTCAGAG 
GAGGAGAGAG GAGCACAAGA TGAGGAGAGA GAAAGACATA GTGGAGATGA GGAGTATGAC 
GATGATGATT ATGAGGAGGA TGAGGACGGC CATCGGGTGA GGAAGCCCAA GCCCACGGAG 
GACATCAGAC AGTCGGAGTC GGACTCAGAC TCGGACCTCG ACGATGTTGA TGCTGTAATG 
GAGCGTCTTC CTGATGACTC AACATCGCTT GTGGACTTTG CCAGAGCGTC GCAGGGCATA 
CTGTTACTGC TGGTGCTCAA ACAACACCTC AAGAACCTCT ACGGCTTCTC TGACGGTAAA 
ATTCAGAAGT ACTCTCCCTC TGAGTCGGCC AAGGTGTACG ATAAAGCCGT AAACAGGAAG 
ACGTTGGCCA ACTTCAACCC ACAGCAGACC ATCGACTTCC TCCGTCATCA TGACGTTCAT 
GGCGAGCTGA CCTACGAGCT GAAGAGGAAG ATCGTCAAAC AGTTCCTCGA CTTTAAGCTG 
TTGATGGAGC ACCTGGACCC TGATGAGGAG GATGAGGATG GAGACACGAG TGCAAACGTG 
AGGAATAAAG CCATCACTGC ACTGCTGGGT GGGGCGGCTG CAAGTCCCCG AAACCACCAC 
ACAGGCGACT CTGAGGAGGA CGACGAGAGG AGTGAAGGAG AGGAGAGGAC CCCCGGGGCA 
TCTCGACGTG GGAGACGAAC TGGAGACTCA GCAGACCTGC TGTCAGCGAA CATGAACGAA 
TCCGTCAGCG CGCTTGATAT CATCGCCATC CACTGCCCCA AATACCGCGA CCGGCCGCAG 
ATCGCCCGCG TCATCCAGAA GAACAGCGAC GGCTACAGCA TCCACTGGAT GGCCGGCTCA 
TACTCCAGCA CGTGGGCCGA GGCCAAAAAG CGTGACGGAC GCAAGCTGGT GCCTTGGGTG 
GACAGCATCA AAGAGACCGA CATCATCTAC AAAAAAATCA CGCTCACCAG TGGCAACAAG 
CTCAACCACA AAGTGGCGCA GACTCTACGT TCACTCTACG CTGCCAAGGA CAGGAACTCC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001233213.1
CDS	429..7574
Translation	MNGDMPHVPITTLAGIAGLTDLLNQLPLPSPLPGTTTKSLLYNGRVAEDVGHLLGCRDETLVSQLANSLSQVSTEHIELKDSLGSDELEGDVPVLLQLLMSRNPNIFRNKTAPNTPQYPAQAGISQQSMAPPYKITHGSMQGSPASANYQQASMSHSPSGHFVPGQSGPGGRFLPQQGSPVPSPYAPQSPATGYRQYPHPPAYSQHQHLQQGSVASPMIPGAMRNVHENKDQMRMGFTSHLLQSSPPYTPPCDGTKDLHLGSQDKQRGQKSSEGEQDSPDKATVYDIVGSPAKDHTKLILRPSRARPAEVELGGMYPGSDPEGELVEALAAIERMESEAAMETERSAKEVQDKDKPLKKRKQDSHPQEPGAAGTAGSGSGAPGGGGGANAGHRLAPQEASAAGTSASRPGLQVSLEQAGRVEDDCMGMPIPASEAQRWPQEPQEGVTPKAVKHEHDHDPEHPHYDDKQPDTPRQKHRPEGRHGDGGAQRAAVQSGSKQVELPPYMLGENTGVLKNFTIPKIRKGELGGGDIPEGWKQPCVRLERLEADVDVKKSVKPVVVLQKLSIDEVQRLMRERDSRASKSGKNRLSSGRSGKGGIDPSVLKDLPPELLAEIESTMPLCERVKMNKRKRSTVNERPKYAEDSSEDEEFSSRKRQRKDRDRTWEAEERDRRSSGEHRRGNFDARRGSGSRYDDSDQDSPPPSLNEVARRLKMKQKKRKVYEPKLTPEEMMDSSTFKRFTLSIDNILENLEDVDFTAQDDDEIPQELLLGKQQLNELGSESAKIKAMGITSRIPSDKLVKLLNILEKNILDGASLSTLMNLDNEGEDEERLWRDLIMERVTKSADACLTALNIMTSTHMPKAVYIEDVIERVLQYTKFHLQNTLYPQYDPVYRVNPKGGSMLSSRAKRAKCSTAKQKVIIMLYNKVCDVVSNISELLEIQLMTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDDEGEPIYIQMVSALVLQLIQCVVHLPADRDSEDDHKKVDDDVFITNSYETARRTAQNFLSVFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPDWPASELLLSLLGRLLVHQFSNKQTEMALRVASLDYLGTVAARLRKDSVTSRMDQKAIERIIRENTEGDETQRLQKALLDYMDENAETDPALAFARKFYIAQWFRDCTTETEKAMRSQNQKEDDSDGAQHAKELQATGDIMQRAETRKKFLHSVVKSTPNQFTTLRMNSDTVDYDDACLIVRYLASTRPFSQSFDIYLTQILRVLGESAIAVRTKAMKCLSEVVAVDPSILARSDMQRGVHGRLMDNSTSVREAAVELLGRFVLSRPQLTEQYYDMLIERILDTGISVRKRVIKILRDICLEQPNFSKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPNHDKETMNRKILNITDVVSACKDTGYDWFEQLLQNLLKSEEDSSYKPTRKACVQLVDNLVEHILKYEEALAEHKSVNSTRLVACITTLYLFSKIRAQLMVKHAMTMQPYLTTKCSSQSDFMVICNVAKILELVVPLMDHPSESFLTTIEEDLMKLILKYGMTVVQYCVSCLGAIVNKVTHNYKFVWACFNRYYGALTKLKVQHQEGTNSMALAATKAALLRSLFTAGALCRHFDFDLEQFKGTTKVVIKEKVLELLLYFTNHEDEEVKCKAIIGLGFLFIMHPSQMFVPEVKTLYNGLLSDKRSSITLKIQVLKNLQMYLQEEDTRMQEADREWQKLSKQEDLKEMGDISSGMSSSIMQLYLKQVLESFFHAQSSVRHFALNVIALTLSQGLIHPVQCVPYLIAMGTDAEPTMRNKADQQLVEIDKKYTGFIHMKAVAGMKMSYQVQQAVFGSAGSVIRGFRQDESNSAQCSHLYSMVRANRQHRRAFLISLLNLFDDSSKMEVNMLLFIADNLAYFPYQSQEEPLFIMHHIDITLSVSGSNLLQTFKESLVKIPGRKSRKRRRRRRRPQRQQPPPPPPQQQQQQNGSEEERGAQDEERERHSGDEEYDDDDYEEDEDGHRVRKPKPTEDIRQSESDSDSDLDDVDAVMERLPDDSTSLVDFARASQGILLLLVLKQHLKNLYGFSDGKIQKYSPSESAKVYDKAVNRKTLANFNPQQTIDFLRHHDVHGELTYELKRKIVKQFLDFKLLMEHLDPDEEDEDGDTSANVRNKAITALLGGAAASPRNHHTGDSEEDDERSEGEERTPGASRRGRRTGDSADLLSANMNESVSALDIIAIHCPKYRDRPQIARVIQKNSDGYSIHWMAGSYSSTWAEAKKRDGRKLVPWVDSIKETDIIYKKITLTSGNKLNHKVAQTLRSLYAAKDRNSS

Target ORF information:

RefSeq Version	NM_001246284.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NIPBL cohesin loading factor a (nipbla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001246284.1

ORF Insert Sequence:

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTGCCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCCAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCACGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGAAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTGAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATC 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCGGA GGATGATCAC AAGAAGGTTG ACGATGATGT TTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCTGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCACT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCTGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTCAGG 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGTGTGAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAAGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATCGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC AAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ACATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAGC AGCAGCAGAA CGGCTCAGAG 
GAGGAGAGAG GAGCACAAGA TGAGGAGAGA GAAAGACATA GTGGAGATGA GGAGTATGAC 
GATGATGATT ATGAGGAGGA TGAGGACGGC CATCGGGTGA GGAAGCCCAA GCCCACGGAG 
GACATCAGAC AGTCGGAGTC GGACTCAGAC TCGGACCTCG ACGATGTTGA TGCTGTAATG 
GAGCGTCTTC CTGATGACTC AACATCGCTT GTGGACTTTG CCAGAGCGTC GCAGGGCATA 
CTGTTACTGC TGGTGCTCAA ACAACACCTC AAGAACCTCT ACGGCTTCTC TGACGGTAAA 
ATTCAGAAGT ACTCTCCCTC TGAGTCGGCC AAGGTGTACG ATAAAGCCGT AAACAGGAAG 
ACGTTGGCCA ACTTCAACCC ACAGCAGACC ATCGACTTCC TCCGTCATCA TGACGTTCAT 
GGCGAGCTGA CCTACGAGCT GAAGAGGAAG ATCGTCAAAC AGTTCCTCGA CTTTAAGCTG 
TTGATGGAGC ACCTGGACCC TGATGAGGAG GATGAGGATG GAGACACGAG TGCAAACGTG 
AGGAATAAAG CCATCACTGC ACTGCTGGGT GGGGCGGCTG CAAGTCCCCG AAACCACCAC 
ACAGGCGACT CTGAGGAGGA CGACGAGAGG AGTGAAGGAG AGGAGAGGAC CCCCGGGGCA 
TCTCGACGTG GGAGACGAAC TGGAGACTCA GCAGACCTGC TGTCAGCGAA CATGAACGAA 
TCCGTCAGCG CGCTTGATAT CATCGCCATC CACTGCCCCA AATACCGCGA CCGGCCGCAG 
ATCGCCCGCG TCATCCAGAA GAACAGCGAC GGCTACAGCA TCCACTGGAT GGCCGGCTCA 
TACTCCAGCA CGTGGGCCGA GGCCAAAAAG CGTGACGGAC GCAAGCTGGT GCCTTGGGTG 
GACAGCATCA AAGAGACCGA CATCATCTAC AAAAAAATCA CGCTCACCAG TGGCAACAAG 
CTCAACCACA AAGTGGCGCA GACTCTACGT TCACTCTACG CTGCCAAGGA CAGGAACTCC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBLA ( XM_009301188.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299463.1 Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.

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CloneID	ODa46051
Clone ID Related Accession (Same CDS sequence)	XM_009301188.3 , XM_009301188.2
Accession Version	XM_009301188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nipped-B-like protein A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTACCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCAAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCATGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGGAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTAAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATT 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCTGA GGATGATCAC AAGAAGGTTG ACGATGATGT GTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCCGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCTCT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCCGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTGAGA 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGCGTAAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAGGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATTGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC TAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ATATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAAC AGCAGCAGCA GAACGGCTCA 
GAGGAGGAGA GAGGAGCACA AGATGAGGAG AGAGAAAGAC ATAGTGGAGA TGAGGAGTAT 
GACGATGATG ATTATGAGGA GGATGAGGAC GGCCATCGGG TGAGGAAGCC CAAGCCCACG 
GAGGACATCA GACAGTCGGA GTCGGACTCA GACTCGGACC TCGACGATGT TGATGCTGTA 
ATGGAGCGTC TTCCTGATGA CTCAACATCG CTTGTGGACT TTGCCAGAGC GTCGCAGGGC 
ATACTGTTAC TGCTGGTGCT CAAACAACAC CTCAAGAACC TCTACGGCTT CTCTGACGGT 
AAAATTCAGA AGTACTCTCC CTCTGAGTCG GCCAAGGTGT ACGATAAAGC CGTAAACAGG 
AAGACGTTGG CCAACTTCAA CCCACAGCAG ACCATCGACT TCCTCCGTCA TCATGACGTT 
CATGGCGAGC TGACCTACGA GCTGAAGAGG AAGATCGTCA AACAGTTCCT CGACTTTAAG 
CTGTTGATGG AGCACCTGGA CCCTGATGAG GAGGATGAAG ATGGAGACAC GAGTGCAAAC 
GTGAGGAATA AAGCCATCAC TGCACTGCTG GGTGGAGCGG CTGCAAGTCC CCGAAACCAC 
CACACAGGCG ACTCTGAGGA GGACGATGAG AGGAGTGAAG GAGAGGAGAG GACCCCCGGG 
GCATCTCGAC GTGGGAGACG AACTGGAGAC TCAGCAGACC TGCTGTCCGC GAACATGAAT 
GAATCCGTCA GCGCGCTTGA TATCATCGCC ATCCACTGCC CCAAATACCG CGACCGGCCG 
CAGATCGCCC GCGTCATCCA GAAGAACAGC GACGGCTACA GCATCCACTG GATGGCCGGC 
TCATACTCCA GCACGTGGGC CGAGGCCAAA AAGCGTGACG GACGCAAGCT GGTGCCTTGG 
GTGGACAGCA TCAAAGAGAC CGACATCATC TACAAAAAAA TCACGCTCAC CAGTGGCAAC 
AAGCTCAACC ACAAAGTGGC GCAGACTCTA CGTTCACTCT ACGCTGCCAA GGACAGGAAC 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299463.1
CDS	566..7714
Translation	MNGDMPHVPITTLAGIAGLTDLLNQLPLPSPLPGTTTKSLLYNGRVAEDVGHLLGCRDETLVSQLANSLSQVSTEHIELKDSLGSDELEGDVPVLLQLLMSRNPNIFRNKTAPNTPQYPAQAGISQQSMAPPYKITHGSMQGSPASANYQQASMSHSPSGHFVPGQSGPGGRFLPQQGSPVPSPYAPQSPATGYRQYPHPPAYSQHQHLQQGSVASPMIPGAMRNVHENKDQMRMGFTSHLLQSSPPYTPPCDGTKDLHLGSQDKQRGQKSSEGEQDSPDKATVYDIVGSPAKDHTKLILRPSRARPAEVELGGMYPGSDPEGELVEALAAIERMESEAAMETERSAKEVQDKDKPLKKRKQDSHPQEPGAAGTAGSGSGAQGGGGGANAGHRLAPQEASAAGTSASRPGLQVSLEQAGRVEDDCMGMPIPASEAQRWPQEPQEGVTPKAVKHEHDHDPEHPHYDDKQPDTPRQKHRPEGRHGDGGAQRAAVQSGSKQVELPPYMLGENTGVLKNFTIPKIRKGELGGGDIPEGWKQPCVRLERLEADVDVKKSVKPVVVLQKLSIDEVQRLMRERDSRASKSGKNRLSSGRSGKGGIDPSVLKDLPPELLAEIESTMPLCERVKMNKRKRSTVNERPKYAEDSSEDEEFSSRKRQRKDRDRTWEAEERDRRSSGEHRRGNFDARRGSGSRYDDSDQDSPPPSLNEVARRLKMKQKKRKVYEPKLTPEEMMDSSTFKRFTLSIDNILENLEDVDFTAQDDDEIPQELLLGKQQLNELGSESAKIKAMGITSRIPSDKLVKLLNILEKNILDGASLSTLMNLDNEGEDEERLWRDLIMERVTKSADACLTALNIMTSTHMPKAVYIEDVIERVLQYTKFHLQNTLYPQYDPVYRVNPKGGSMLSSRAKRAKCSTAKQKVIIMLYNKVCDVVSNISELLEIQLMTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDDEGEPIYIQMVSALVLQLIQCVVHLPADRDSEDDHKKVDDDVFITNSYETARRTAQNFLSVFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPDWPASELLLSLLGRLLVHQFSNKQTEMALRVASLDYLGTVAARLRKDSVTSRMDQKAIERIIRENTEGDETQRLQKALLDYMDENAETDPALAFARKFYIAQWFRDCTTETEKAMRSQNQKEDDSDGAQHAKELQATGDIMQRAETRKKFLHSVVKSTPNQFTTLRMNSDTVDYDDACLIVRYLASTRPFSQSFDIYLTQILRVLGESAIAVRTKAMKCLSEVVAVDPSILARSDMQRGVHGRLMDNSTSVREAAVELLGRFVLSRPQLTEQYYDMLIERILDTGISVRKRVIKILRDICLEQPNFSKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPNHDKETMNRKILNITDVVSACKDTGYDWFEQLLQNLLKSEEDSSYKPTRKACVQLVDNLVEHILKYEEALAEHKSVNSTRLVACITTLYLFSKIRAQLMVKHAMTMQPYLTTKCSSQSDFMVICNVAKILELVVPLMDHPSESFLTTIEEDLMKLILKYGMTVVQYCVSCLGAIVNKVTHNYKFVWACFNRYYGALTKLKVQHQEGTNSMALAATKAALLRSLFTAGALCRHFDFDLEQFKGTTKVVIKEKVLELLLYFTNHEDEEVKCKAIIGLGFLFIMHPSQMFVPEVKTLYNGLLSDKRSSITLKIQVLKNLQMYLQEEDTRMQEADREWQKLSKQEDLKEMGDISSGMSSSIMQLYLKQVLESFFHAQSSVRHFALNVIALTLSQGLIHPVQCVPYLIAMGTDAEPTMRNKADQQLVEIDKKYTGFIHMKAVAGMKMSYQVQQAVFGSAGSVIRGFRQDESNSAQCSHLYSMVRANRQHRRAFLISLLNLFDDSSKMEVNMLLFIADNLAYFPYQSQEEPLFIMHHIDITLSVSGSNLLQTFKESLVKIPGRKSRKRRRRRRRPQRQQPPPPPPQQQQQQQNGSEEERGAQDEERERHSGDEEYDDDDYEEDEDGHRVRKPKPTEDIRQSESDSDSDLDDVDAVMERLPDDSTSLVDFARASQGILLLLVLKQHLKNLYGFSDGKIQKYSPSESAKVYDKAVNRKTLANFNPQQTIDFLRHHDVHGELTYELKRKIVKQFLDFKLLMEHLDPDEEDEDGDTSANVRNKAITALLGGAAASPRNHHTGDSEEDDERSEGEERTPGASRRGRRTGDSADLLSANMNESVSALDIIAIHCPKYRDRPQIARVIQKNSDGYSIHWMAGSYSSTWAEAKKRDGRKLVPWVDSIKETDIIYKKITLTSGNKLNHKVAQTLRSLYAAKDRNSS

Target ORF information:

RefSeq Version	XM_009301188.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301188.3

ORF Insert Sequence:

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTACCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCAAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCATGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGGAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTAAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATT 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCTGA GGATGATCAC AAGAAGGTTG ACGATGATGT GTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCCGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCTCT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCCGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTGAGA 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGCGTAAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAGGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATTGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC TAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ATATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAAC AGCAGCAGCA GAACGGCTCA 
GAGGAGGAGA GAGGAGCACA AGATGAGGAG AGAGAAAGAC ATAGTGGAGA TGAGGAGTAT 
GACGATGATG ATTATGAGGA GGATGAGGAC GGCCATCGGG TGAGGAAGCC CAAGCCCACG 
GAGGACATCA GACAGTCGGA GTCGGACTCA GACTCGGACC TCGACGATGT TGATGCTGTA 
ATGGAGCGTC TTCCTGATGA CTCAACATCG CTTGTGGACT TTGCCAGAGC GTCGCAGGGC 
ATACTGTTAC TGCTGGTGCT CAAACAACAC CTCAAGAACC TCTACGGCTT CTCTGACGGT 
AAAATTCAGA AGTACTCTCC CTCTGAGTCG GCCAAGGTGT ACGATAAAGC CGTAAACAGG 
AAGACGTTGG CCAACTTCAA CCCACAGCAG ACCATCGACT TCCTCCGTCA TCATGACGTT 
CATGGCGAGC TGACCTACGA GCTGAAGAGG AAGATCGTCA AACAGTTCCT CGACTTTAAG 
CTGTTGATGG AGCACCTGGA CCCTGATGAG GAGGATGAAG ATGGAGACAC GAGTGCAAAC 
GTGAGGAATA AAGCCATCAC TGCACTGCTG GGTGGAGCGG CTGCAAGTCC CCGAAACCAC 
CACACAGGCG ACTCTGAGGA GGACGATGAG AGGAGTGAAG GAGAGGAGAG GACCCCCGGG 
GCATCTCGAC GTGGGAGACG AACTGGAGAC TCAGCAGACC TGCTGTCCGC GAACATGAAT 
GAATCCGTCA GCGCGCTTGA TATCATCGCC ATCCACTGCC CCAAATACCG CGACCGGCCG 
CAGATCGCCC GCGTCATCCA GAAGAACAGC GACGGCTACA GCATCCACTG GATGGCCGGC 
TCATACTCCA GCACGTGGGC CGAGGCCAAA AAGCGTGACG GACGCAAGCT GGTGCCTTGG 
GTGGACAGCA TCAAAGAGAC CGACATCATC TACAAAAAAA TCACGCTCAC CAGTGGCAAC 
AAGCTCAACC ACAAAGTGGC GCAGACTCTA CGTTCACTCT ACGCTGCCAA GGACAGGAAC 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBLA ( XM_009301188.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299463.1 Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.

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CloneID	ODa46051
Clone ID Related Accession (Same CDS sequence)	XM_009301188.3 , XM_009301188.2
Accession Version	XM_009301188.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nipped-B-like protein A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTACCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCAAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCATGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGGAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTAAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATT 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCTGA GGATGATCAC AAGAAGGTTG ACGATGATGT GTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCCGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCTCT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCCGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTGAGA 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGCGTAAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAGGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATTGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC TAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ATATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAAC AGCAGCAGCA GAACGGCTCA 
GAGGAGGAGA GAGGAGCACA AGATGAGGAG AGAGAAAGAC ATAGTGGAGA TGAGGAGTAT 
GACGATGATG ATTATGAGGA GGATGAGGAC GGCCATCGGG TGAGGAAGCC CAAGCCCACG 
GAGGACATCA GACAGTCGGA GTCGGACTCA GACTCGGACC TCGACGATGT TGATGCTGTA 
ATGGAGCGTC TTCCTGATGA CTCAACATCG CTTGTGGACT TTGCCAGAGC GTCGCAGGGC 
ATACTGTTAC TGCTGGTGCT CAAACAACAC CTCAAGAACC TCTACGGCTT CTCTGACGGT 
AAAATTCAGA AGTACTCTCC CTCTGAGTCG GCCAAGGTGT ACGATAAAGC CGTAAACAGG 
AAGACGTTGG CCAACTTCAA CCCACAGCAG ACCATCGACT TCCTCCGTCA TCATGACGTT 
CATGGCGAGC TGACCTACGA GCTGAAGAGG AAGATCGTCA AACAGTTCCT CGACTTTAAG 
CTGTTGATGG AGCACCTGGA CCCTGATGAG GAGGATGAAG ATGGAGACAC GAGTGCAAAC 
GTGAGGAATA AAGCCATCAC TGCACTGCTG GGTGGAGCGG CTGCAAGTCC CCGAAACCAC 
CACACAGGCG ACTCTGAGGA GGACGATGAG AGGAGTGAAG GAGAGGAGAG GACCCCCGGG 
GCATCTCGAC GTGGGAGACG AACTGGAGAC TCAGCAGACC TGCTGTCCGC GAACATGAAT 
GAATCCGTCA GCGCGCTTGA TATCATCGCC ATCCACTGCC CCAAATACCG CGACCGGCCG 
CAGATCGCCC GCGTCATCCA GAAGAACAGC GACGGCTACA GCATCCACTG GATGGCCGGC 
TCATACTCCA GCACGTGGGC CGAGGCCAAA AAGCGTGACG GACGCAAGCT GGTGCCTTGG 
GTGGACAGCA TCAAAGAGAC CGACATCATC TACAAAAAAA TCACGCTCAC CAGTGGCAAC 
AAGCTCAACC ACAAAGTGGC GCAGACTCTA CGTTCACTCT ACGCTGCCAA GGACAGGAAC 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299463.1
CDS	580..7728
Translation	MNGDMPHVPITTLAGIAGLTDLLNQLPLPSPLPGTTTKSLLYNGRVAEDVGHLLGCRDETLVSQLANSLSQVSTEHIELKDSLGSDELEGDVPVLLQLLMSRNPNIFRNKTAPNTPQYPAQAGISQQSMAPPYKITHGSMQGSPASANYQQASMSHSPSGHFVPGQSGPGGRFLPQQGSPVPSPYAPQSPATGYRQYPHPPAYSQHQHLQQGSVASPMIPGAMRNVHENKDQMRMGFTSHLLQSSPPYTPPCDGTKDLHLGSQDKQRGQKSSEGEQDSPDKATVYDIVGSPAKDHTKLILRPSRARPAEVELGGMYPGSDPEGELVEALAAIERMESEAAMETERSAKEVQDKDKPLKKRKQDSHPQEPGAAGTAGSGSGAQGGGGGANAGHRLAPQEASAAGTSASRPGLQVSLEQAGRVEDDCMGMPIPASEAQRWPQEPQEGVTPKAVKHEHDHDPEHPHYDDKQPDTPRQKHRPEGRHGDGGAQRAAVQSGSKQVELPPYMLGENTGVLKNFTIPKIRKGELGGGDIPEGWKQPCVRLERLEADVDVKKSVKPVVVLQKLSIDEVQRLMRERDSRASKSGKNRLSSGRSGKGGIDPSVLKDLPPELLAEIESTMPLCERVKMNKRKRSTVNERPKYAEDSSEDEEFSSRKRQRKDRDRTWEAEERDRRSSGEHRRGNFDARRGSGSRYDDSDQDSPPPSLNEVARRLKMKQKKRKVYEPKLTPEEMMDSSTFKRFTLSIDNILENLEDVDFTAQDDDEIPQELLLGKQQLNELGSESAKIKAMGITSRIPSDKLVKLLNILEKNILDGASLSTLMNLDNEGEDEERLWRDLIMERVTKSADACLTALNIMTSTHMPKAVYIEDVIERVLQYTKFHLQNTLYPQYDPVYRVNPKGGSMLSSRAKRAKCSTAKQKVIIMLYNKVCDVVSNISELLEIQLMTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDDEGEPIYIQMVSALVLQLIQCVVHLPADRDSEDDHKKVDDDVFITNSYETARRTAQNFLSVFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPDWPASELLLSLLGRLLVHQFSNKQTEMALRVASLDYLGTVAARLRKDSVTSRMDQKAIERIIRENTEGDETQRLQKALLDYMDENAETDPALAFARKFYIAQWFRDCTTETEKAMRSQNQKEDDSDGAQHAKELQATGDIMQRAETRKKFLHSVVKSTPNQFTTLRMNSDTVDYDDACLIVRYLASTRPFSQSFDIYLTQILRVLGESAIAVRTKAMKCLSEVVAVDPSILARSDMQRGVHGRLMDNSTSVREAAVELLGRFVLSRPQLTEQYYDMLIERILDTGISVRKRVIKILRDICLEQPNFSKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPNHDKETMNRKILNITDVVSACKDTGYDWFEQLLQNLLKSEEDSSYKPTRKACVQLVDNLVEHILKYEEALAEHKSVNSTRLVACITTLYLFSKIRAQLMVKHAMTMQPYLTTKCSSQSDFMVICNVAKILELVVPLMDHPSESFLTTIEEDLMKLILKYGMTVVQYCVSCLGAIVNKVTHNYKFVWACFNRYYGALTKLKVQHQEGTNSMALAATKAALLRSLFTAGALCRHFDFDLEQFKGTTKVVIKEKVLELLLYFTNHEDEEVKCKAIIGLGFLFIMHPSQMFVPEVKTLYNGLLSDKRSSITLKIQVLKNLQMYLQEEDTRMQEADREWQKLSKQEDLKEMGDISSGMSSSIMQLYLKQVLESFFHAQSSVRHFALNVIALTLSQGLIHPVQCVPYLIAMGTDAEPTMRNKADQQLVEIDKKYTGFIHMKAVAGMKMSYQVQQAVFGSAGSVIRGFRQDESNSAQCSHLYSMVRANRQHRRAFLISLLNLFDDSSKMEVNMLLFIADNLAYFPYQSQEEPLFIMHHIDITLSVSGSNLLQTFKESLVKIPGRKSRKRRRRRRRPQRQQPPPPPPQQQQQQQNGSEEERGAQDEERERHSGDEEYDDDDYEEDEDGHRVRKPKPTEDIRQSESDSDSDLDDVDAVMERLPDDSTSLVDFARASQGILLLLVLKQHLKNLYGFSDGKIQKYSPSESAKVYDKAVNRKTLANFNPQQTIDFLRHHDVHGELTYELKRKIVKQFLDFKLLMEHLDPDEEDEDGDTSANVRNKAITALLGGAAASPRNHHTGDSEEDDERSEGEERTPGASRRGRRTGDSADLLSANMNESVSALDIIAIHCPKYRDRPQIARVIQKNSDGYSIHWMAGSYSSTWAEAKKRDGRKLVPWVDSIKETDIIYKKITLTSGNKLNHKVAQTLRSLYAAKDRNSS

Target ORF information:

RefSeq Version	XM_009301188.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nipped-B homolog a (Drosophila) (nipbla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301188.2

ORF Insert Sequence:

ATGAATGGGG ACATGCCACA TGTGCCCATT ACAACCCTTG CTGGGATCGC TGGTCTCACT 
GACTTGTTGA ACCAGCTGCC CCTGCCCTCC CCTTTACCGG GCACCACCAC TAAGAGCCTG 
CTGTACAATG GGCGAGTAGC TGAGGACGTG GGGCATCTGT TGGGCTGCAG GGATGAAACT 
CTGGTCTCTC AGCTCGCCAA CAGCCTCAGC CAGGTCTCCA CGGAGCACAT AGAGCTGAAG 
GACAGCCTGG GCAGTGATGA GCTTGAGGGA GATGTACCAG TGCTGCTGCA GCTTTTGATG 
TCCAGAAACC CTAACATCTT CAGGAATAAG ACTGCACCAA ACACTCCACA GTATCCGGCC 
CAAGCGGGTA TAAGTCAGCA GTCAATGGCA CCGCCGTATA AAATCACACA TGGCTCCATG 
CAGGGAAGCC CAGCCTCTGC CAATTATCAG CAGGCCTCCA TGTCCCACAG TCCCTCTGGG 
CATTTTGTAC CGGGCCAGTC TGGTCCTGGT GGACGGTTTC TTCCTCAGCA GGGCAGTCCA 
GTGCCCAGCC CATATGCCCC CCAGAGCCCT GCTACTGGTT ACAGACAGTA CCCTCACCCT 
CCAGCCTATA GTCAACACCA GCACCTACAG CAAGGTTCAG TAGCAAGTCC CATGATCCCA 
GGAGCCATGA GAAATGTGCA TGAAAATAAG GATCAAATGA GGATGGGTTT CACGTCTCAC 
TTACTGCAGT CATCTCCACC GTACACCCCT CCCTGCGACG GGACTAAAGA CCTGCACCTG 
GGCTCCCAGG ACAAACAACG AGGTCAGAAG TCCAGCGAGG GAGAGCAGGA CTCCCCTGAT 
AAGGCCACCG TGTACGATAT TGTGGGCTCG CCAGCGAAAG ACCACACTAA GCTGATCCTG 
AGGCCGTCAC GGGCGAGGCC AGCCGAGGTG GAGCTTGGGG GAATGTACCC AGGCTCAGAC 
CCAGAAGGAG AGCTCGTGGA GGCTCTTGCT GCTATAGAGA GGATGGAGAG TGAAGCAGCG 
ATGGAGACTG AACGCAGTGC TAAAGAGGTG CAGGACAAAG ATAAGCCTTT GAAGAAGCGC 
AAGCAGGACT CTCACCCCCA GGAGCCTGGC GCAGCTGGCA CAGCAGGTTC GGGCTCTGGG 
GCTCAAGGTG GAGGAGGAGG AGCTAATGCA GGCCACAGGC TGGCTCCTCA GGAGGCCAGT 
GCTGCAGGCA CCAGCGCCAG CCGCCCGGGT CTGCAGGTGA GCTTGGAGCA AGCAGGCAGA 
GTGGAGGACG ACTGCATGGG TATGCCCATC CCTGCAAGCG AGGCGCAGCG CTGGCCTCAG 
GAGCCACAGG AGGGCGTCAC CCCAAAGGCC GTCAAACACG AGCACGACCA TGATCCAGAA 
CACCCGCATT ATGATGACAA ACAGCCCGAT ACCCCACGGC AAAAACATCG ACCCGAGGGC 
CGGCATGGCG ATGGAGGTGC ACAGCGAGCG GCGGTTCAAT CAGGAAGTAA ACAGGTCGAG 
CTTCCTCCGT ACATGCTGGG TGAAAACACG GGAGTGCTTA AGAATTTCAC CATTCCCAAA 
ATCAGGAAAG GGGAGCTCGG GGGCGGTGAT ATTCCTGAAG GATGGAAGCA GCCTTGCGTG 
CGGCTGGAGA GACTAGAAGC GGACGTGGAC GTGAAGAAGA GCGTCAAGCC TGTGGTGGTT 
CTGCAGAAAC TCTCCATCGA TGAGGTGCAG AGGCTGATGA GGGAGAGAGA CTCACGGGCC 
TCCAAGTCCG GCAAGAACAG GCTCTCCTCT GGGAGATCTG GGAAAGGTGG AATAGACCCG 
TCTGTGCTGA AGGATCTTCC TCCTGAACTG CTGGCAGAGA TCGAGTCCAC CATGCCTCTG 
TGTGAGCGGG TGAAGATGAA CAAACGGAAA CGCAGCACTG TTAATGAGAG GCCTAAATAT 
GCAGAGGACA GCTCAGAGGA TGAGGAGTTC TCTTCTCGTA AACGTCAGAG GAAGGACCGG 
GATCGAACAT GGGAGGCTGA AGAGCGAGAC CGGAGGAGTT CAGGAGAGCA TCGAAGAGGA 
AACTTTGATG CACGAAGAGG CTCCGGCAGC CGATACGACG ACTCGGACCA AGATTCACCT 
CCACCCAGCC TTAACGAAGT TGCAAGACGT TTAAAGATGA AGCAGAAGAA GAGGAAAGTG 
TATGAGCCCA AGCTGACTCC TGAAGAAATG ATGGACTCTT CCACCTTTAA GAGGTTCACG 
TTAAGTATTG ACAACATCTT GGAGAATCTC GAGGATGTGG ACTTTACAGC TCAGGATGAT 
GATGAGATCC CTCAGGAGCT GTTGTTGGGC AAGCAACAGC TAAATGAATT GGGCAGTGAA 
TCTGCTAAGA TAAAAGCCAT GGGCATCACG AGCAGGATTC CTTCTGATAA ACTCGTGAAA 
CTGTTGAACA TCCTGGAGAA GAACATTCTG GATGGAGCCA GTCTTTCCAC ACTCATGAAC 
CTTGATAATG AGGGTGAGGA TGAGGAGCGT CTCTGGCGTG ATCTCATCAT GGAGCGAGTG 
ACCAAATCAG CAGACGCGTG TCTGACAGCA CTGAACATCA TGACATCGAC ACACATGCCC 
AAAGCCGTGT ACATTGAGGA CGTGATCGAG AGGGTTTTAC AGTACACTAA GTTCCACTTG 
CAGAACACGC TTTATCCGCA GTATGATCCA GTCTACAGAG TCAACCCTAA AGGAGGTTCC 
ATGCTCAGCT CCAGAGCGAA GCGAGCGAAG TGTTCCACTG CCAAGCAGAA GGTCATCATT 
ATGCTCTATA ACAAAGTGTG TGACGTGGTC AGCAACATCT CAGAGCTGCT GGAAATTCAG 
CTGATGACTG ATACAACAAT ATTGCAGGTC TCTTCGATGG GCATCACTCC GTTCTTTGTG 
GAGAATGTCA GTGAGCTGCA GCTGTGTGCC ATAAAGCTGG TGACGGCGGT CTTCTCTCGT 
TACGAGAAGC ACAGGCAGTT AATCCTAGAG GAGATCTTTA CCTCTTTGGC TAGATTGCCC 
ACCAGCAAAC GCAGCCTCAG GAACTTCAGG TTGAACAGCA GCGATGATGA GGGTGAACCC 
ATATACATAC AGATGGTTAG TGCTCTAGTG CTGCAGTTAA TCCAGTGTGT GGTCCATTTG 
CCGGCTGACA GAGACTCTGA GGATGATCAC AAGAAGGTTG ACGATGATGT GTTCATTACC 
AACTCGTATG AAACAGCCAG GCGGACTGCT CAGAACTTCC TCTCCGTCTT CCTCAAGAAG 
TGTGGCAGTA AACAGGGTGA GGAAGACTAC AGGCCGCTGT TTGAGAACTT TGTGCAGGAT 
CTGCTGTCAA CTGTCAACAA ACCCGACTGG CCCGCTTCAG AATTACTGCT GAGCCTTCTG 
GGAAGATTAC TGGTGCATCA GTTCAGTAAT AAGCAGACAG AGATGGCTCT GCGAGTGGCA 
TCGCTGGATT ATCTCGGTAC CGTAGCAGCC AGATTGAGAA AAGATTCCGT CACCAGCAGG 
ATGGACCAGA AAGCCATAGA ACGCATCATC AGAGAGAACA CCGAGGGAGA TGAGACTCAG 
CGGTTACAGA AAGCTCTGCT GGATTATATG GATGAGAATG CAGAAACAGA CCCTGCTCTT 
GCATTTGCAC GTAAATTTTA CATCGCTCAG TGGTTCCGAG ACTGCACTAC GGAGACCGAG 
AAGGCCATGA GGAGTCAGAA TCAGAAGGAG GATGATTCAG ACGGGGCACA GCACGCCAAA 
GAGCTCCAGG CCACAGGAGA CATCATGCAG CGCGCTGAAA CCCGCAAGAA ATTCCTCCAC 
TCCGTTGTTA AATCAACTCC TAATCAATTT ACTACTCTCA GGATGAATTC AGACACTGTG 
GACTATGACG ACGCCTGCCT TATTGTTCGC TACCTGGCCT CCACCAGACC CTTCTCTCAG 
AGCTTTGACA TCTACCTGAC ACAGATTCTA AGAGTGTTAG GGGAGAGCGC CATAGCTGTC 
AGGACCAAAG CCATGAAGTG TCTGTCCGAG GTTGTGGCTG TTGACCCAAG TATACTGGCC 
AGGTCTGATA TGCAGCGTGG TGTTCACGGG CGATTGATGG ATAACTCCAC AAGTGTGAGA 
GAAGCAGCGG TCGAGCTGCT GGGACGCTTT GTGTTGAGTC GACCTCAGCT GACGGAGCAG 
TACTACGACA TGCTCATCGA GAGGATACTG GACACCGGTA TCAGCGTAAG GAAGAGGGTG 
ATCAAGATTC TGAGGGACAT CTGTTTAGAG CAGCCCAACT TCAGCAAGAT CACAGAGATG 
TGTGTAAAGA TGATCCGCAG GGTCAATGAC GAGGAAGGCA TCAAGAAACT GGTGAATGAA 
ACATTCCAGA AGCTGTGGTT TACTCCCACA CCCAACCACG ACAAAGAGAC GATGAATAGG 
AAGATTCTCA ACATCACTGA TGTGGTCTCT GCCTGCAAGG ACACTGGCTA TGACTGGTTT 
GAGCAGCTCC TGCAGAACCT TTTAAAGTCA GAGGAGGATT CGTCCTATAA GCCAACCAGG 
AAGGCCTGCG TGCAGTTGGT TGACAATCTT GTGGAGCACA TCCTCAAATA TGAGGAGGCA 
CTTGCAGAGC ATAAAAGTGT GAACTCGACG CGGTTGGTGG CGTGCATCAC CACGCTGTAT 
CTGTTCAGTA AGATCAGAGC GCAGCTGATG GTCAAGCATG CTATGACTAT GCAGCCCTAC 
CTCACCACCA AGTGCAGTTC TCAGAGTGAC TTCATGGTGA TTTGTAATGT TGCAAAAATC 
CTAGAGCTGG TGGTTCCTCT GATGGATCAT CCCAGCGAGA GCTTCCTGAC CACCATTGAA 
GAAGACCTGA TGAAGCTCAT CCTAAAATAT GGCATGACCG TTGTCCAGTA CTGTGTGAGC 
TGTTTGGGTG CGATTGTGAA TAAAGTCACC CATAACTATA AGTTTGTGTG GGCCTGTTTT 
AACCGTTATT ATGGGGCTCT TACTAAGTTA AAGGTCCAGC ATCAGGAGGG AACGAACAGC 
ATGGCGTTGG CTGCTACCAA AGCGGCCCTG CTCAGATCTC TGTTCACCGC CGGCGCGCTC 
TGCAGACACT TCGACTTTGA TCTGGAGCAG TTCAAGGGCA CTACTAAGGT TGTAATAAAG 
GAGAAGGTTC TGGAATTATT ACTGTATTTC ACCAATCATG AGGACGAGGA AGTTAAATGC 
AAAGCCATCA TCGGGTTGGG CTTCCTATTC ATCATGCACC CGAGTCAGAT GTTCGTTCCT 
GAGGTGAAGA CCCTGTATAA CGGGCTTCTC TCTGATAAAC GGAGCTCCAT CACACTGAAG 
ATCCAGGTGT TGAAGAACCT TCAGATGTAC CTTCAGGAAG AGGATACTCG CATGCAGGAA 
GCAGACAGAG AATGGCAGAA ACTGTCGAAG CAGGAGGACC TGAAAGAGAT GGGTGACATC 
TCCTCTGGTA TGAGCAGCTC CATCATGCAG CTTTATTTGA AGCAGGTGCT GGAGTCGTTT 
TTCCATGCAC AGTCCAGTGT ACGACACTTT GCCCTCAATG TCATCGCTCT GACACTCAGC 
CAGGGACTCA TACACCCTGT ACAGTGTGTG CCATATCTGA TCGCGATGGG CACAGACGCA 
GAGCCCACCA TGAGGAACAA AGCAGACCAG CAGCTGGTGG AGATTGATAA GAAGTACACT 
GGATTCATCC ATATGAAAGC TGTAGCAGGG ATGAAGATGT CGTACCAGGT GCAGCAGGCC 
GTCTTTGGAT CAGCCGGGTC TGTGATCCGA GGTTTCCGTC AGGATGAGTC TAATTCAGCT 
CAGTGTTCGC ACCTCTACAG CATGGTCCGC GCAAACAGAC AACACCGGCG AGCGTTTCTC 
ATCTCGCTGC TCAATCTCTT CGACGACAGC TCGAAGATGG AGGTGAACAT GCTGTTGTTC 
ATCGCTGATA ATCTGGCCTA CTTCCCCTAT CAGAGTCAGG AGGAGCCGCT GTTCATCATG 
CATCATATAG ATATCACACT GTCTGTATCG GGGAGCAACC TGCTGCAGAC CTTTAAAGAG 
TCTCTGGTTA AGATACCAGG CAGGAAAAGC AGGAAAAGGA GACGACGGCG GAGGCGACCG 
CAACGCCAGC AACCACCACC ACCACCACCA CAACAACAAC AGCAGCAGCA GAACGGCTCA 
GAGGAGGAGA GAGGAGCACA AGATGAGGAG AGAGAAAGAC ATAGTGGAGA TGAGGAGTAT 
GACGATGATG ATTATGAGGA GGATGAGGAC GGCCATCGGG TGAGGAAGCC CAAGCCCACG 
GAGGACATCA GACAGTCGGA GTCGGACTCA GACTCGGACC TCGACGATGT TGATGCTGTA 
ATGGAGCGTC TTCCTGATGA CTCAACATCG CTTGTGGACT TTGCCAGAGC GTCGCAGGGC 
ATACTGTTAC TGCTGGTGCT CAAACAACAC CTCAAGAACC TCTACGGCTT CTCTGACGGT 
AAAATTCAGA AGTACTCTCC CTCTGAGTCG GCCAAGGTGT ACGATAAAGC CGTAAACAGG 
AAGACGTTGG CCAACTTCAA CCCACAGCAG ACCATCGACT TCCTCCGTCA TCATGACGTT 
CATGGCGAGC TGACCTACGA GCTGAAGAGG AAGATCGTCA AACAGTTCCT CGACTTTAAG 
CTGTTGATGG AGCACCTGGA CCCTGATGAG GAGGATGAAG ATGGAGACAC GAGTGCAAAC 
GTGAGGAATA AAGCCATCAC TGCACTGCTG GGTGGAGCGG CTGCAAGTCC CCGAAACCAC 
CACACAGGCG ACTCTGAGGA GGACGATGAG AGGAGTGAAG GAGAGGAGAG GACCCCCGGG 
GCATCTCGAC GTGGGAGACG AACTGGAGAC TCAGCAGACC TGCTGTCCGC GAACATGAAT 
GAATCCGTCA GCGCGCTTGA TATCATCGCC ATCCACTGCC CCAAATACCG CGACCGGCCG 
CAGATCGCCC GCGTCATCCA GAAGAACAGC GACGGCTACA GCATCCACTG GATGGCCGGC 
TCATACTCCA GCACGTGGGC CGAGGCCAAA AAGCGTGACG GACGCAAGCT GGTGCCTTGG 
GTGGACAGCA TCAAAGAGAC CGACATCATC TACAAAAAAA TCACGCTCAC CAGTGGCAAC 
AAGCTCAACC ACAAAGTGGC GCAGACTCTA CGTTCACTCT ACGCTGCCAA GGACAGGAAC 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

L-leucine partially rescues translational and developmental defects associated with zebrafish models of Cornelia de Lange syndrome.
Human molecular genetics24(6)1540-55(2015 Mar)
Xu B,Sowa N,Cardenas ME,Gerton JL

Nipbl and mediator cooperatively regulate gene expression to control limb development.
PLoS genetics10(9)e1004671(2014 Sep)
Muto A,Ikeda S,Lopez-Burks ME,Kikuchi Y,Calof AL,Lander AD,Schilling TF

From zebrafish heart jogging genes to mouse and human orthologs: using Gene Ontology to investigate mammalian heart development.
F1000Research2242(2013)
Khodiyar VK,Howe D,Talmud PJ,Breckenridge R,Lovering RC

Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome.
PLoS biology9(10)e1001181(2011 Oct)
Muto A,Calof AL,Lander AD,Schilling TF

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NIPBLA cDNA ORF clone, Danio rerio(Zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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