sympk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sympk
Entrez Gene ID	570858
Full Name	symplekin
Synonyms	si:dkeyp-117b8.1
General protein information	Preferred Names symplekin Names symplekin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_694383.8	XP_699475.5	symplekin isoform X1
XM_694383.7	XP_699475.5	symplekin isoform X1
XM_005165573.3	XP_005165630.1	symplekin isoform X2
XM_005165573.4	XP_005165630.1	symplekin isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkeyp-117b8.1	XP_005165630.1
Bos taurus (cattle)	SYMPK	NP_001193842.1
Homo sapiens (human)	SYMPK	NP_004810.2
Caenorhabditis elegans (roundworm)	symk-1	NP_505210.2
Mus musculus (house mouse)	Sympk	NP_080881.2
Rattus norvegicus (Norway rat)	Sympk	NP_001094300.1
Canis lupus familiaris (dog)	SYMPK	XP_533638.2
Drosophila melanogaster (fruit fly)	Sym	NP_649580.1
Xenopus tropicalis (tropical clawed frog)	sympk	NP_989259.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002618	XP_312315.5
Pan troglodytes (chimpanzee)	SYMPK	XP_001166898.1

Gene Ontology

The following sympk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sympk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11112	XM_694383.8 Latest version!	Danio rerio symplekin (sympk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa11113	XM_005165573.4 Latest version!	Danio rerio symplekin (sympk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa11112	XM_694383.7	Danio rerio symplekin (sympk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa11113	XM_005165573.3	Danio rerio symplekin (sympk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sympk ( XM_694383.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_699475.5 Danio rerio symplekin (sympk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11112
Clone ID Related Accession (Same CDS sequence)	XM_694383.8 , XM_694383.7
Accession Version	XM_694383.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_694383.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGCAGAT GGATGTGTCC 
GAGGAGGCTC CACAGTCCAG CCCGGTAGCA GAAAAGGCAG ATGAAAGCAT GGACGAGTCG 
ACTGCAGGAC CTGCAGACGA GGCTGAAGAA CCCGTAGAAG AGCCAGAGGA AGAGCATGCA 
GAAACTGGAG AAAGCAGTGC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699475.5
CDS	160..3906
Translation	MMAGEQASGASSQIHSNEEDKAFDMTTSERVVDLLNQAALISTEEKLIVLKQVQELVINKDPSLLDNFLDEIIAFQTDKSIEVRKFVIGFIEEACKRDNELLLRLIANLNMLMRDESVNVVKKAILTLTQLYKVALQWLVRTKSVTDMQEACWDMVTQMKGDVLALLDSDNDGVRTHAIKFTESLIITLSPRTPDSDTPKKQEGDISLDKIPKDHTYIRYDTLCEEGKTALDQLLKFMVHPAISSINLTTALGSLATLARQRPMFMSEVVQAYEMLHANLPPTLAKSQVSSVRKNLKLHLVAVLKHPSSVDFHVQISTLLQDLGMSPSEITRCTPVAVQPRKRLHHEPYVEGKRIKMEAVEDDEDKEEPAPVVTPKPSSSIGTQSAIDLTAEFLLPLLTSDNVANLVLISMVYLPEVMPASFQATYTPVESAGTDAQIKHLARLMATQMTAAGLGPGLEQCKARDEEVKEEGAAGESSSKDPLIIRKLSAVSLGQAISVVGASAAYKSPIIEEAPQIKKLPEPILPSTQLKAPGASGRKKVFKLADVVQPFSEVQIEKLANMAIKRILYSEKAIAQTGMSHVRVKLLARLVTQFEGTKKNDLLQFVLEDMRSRSELAFSLLYHEYNEYLSQEPSGCLDSYEHCLFTLLSGLQERPEQRDGLFTKIVLEAPIITDSALEVIQRYCEDESRVYLGMSTLKELIFKRPSRQYQYLNVLLNLTSHEKEKVRSTALSFIKRMYEKEHLKDCIEKFALTYMQFLVHPNPPSLLFGAGEDTEVAAPWTEETVRQCLYLYLSLLPLNHRLVHELAAVYTEAIADIKRSVLRVIEQPIRGMGMNSPDLLLLVENCPKGAETLVTRCLHILTDKVPPSPELVERVRDLYHKRVPDVRFLIPVINGLEKNEVIQALPKLIKLNPIVVKEVFNRLLGTQHSEGSSSMSPLTPGELLIALHNIDSSKCDMKSIIKATNLCFGEKNVYTSEVLAVVMQQLMEQTPLPILLMRTVIQSLTMYPRLAGFVMNILARLILKQVWKYPKVWEGFVKCCQRTKPQSYNVLLQLPPPQLASVFERCPEMREPLLQHVHSFTPHQQAHIPTSIMTVLEATNRKPEPEPQPEIQHPEVMEEREVPLPKHISVHAPVQQAEPPAPKPIVFTEPMPPQEKHHHHHQVPSRREVAEVAEPMEQEETAHVPEEDTSEAPPQQMDVSEEAPQSSPVAEKADESMDESTAGPADEAEEPVEEPEEEHAETGESSAD

Target ORF information:

RefSeq Version	XM_694383.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio symplekin (sympk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694383.8

ORF Insert Sequence:

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGCAGAT GGATGTGTCC 
GAGGAGGCTC CACAGTCCAG CCCGGTAGCA GAAAAGGCAG ATGAAAGCAT GGACGAGTCG 
ACTGCAGGAC CTGCAGACGA GGCTGAAGAA CCCGTAGAAG AGCCAGAGGA AGAGCATGCA 
GAAACTGGAG AAAGCAGTGC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sympk ( XM_694383.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_699475.5 Danio rerio symplekin (sympk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11112
Clone ID Related Accession (Same CDS sequence)	XM_694383.8 , XM_694383.7
Accession Version	XM_694383.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	symplekin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_694383.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGCAGAT GGATGTGTCC 
GAGGAGGCTC CACAGTCCAG CCCGGTAGCA GAAAAGGCAG ATGAAAGCAT GGACGAGTCG 
ACTGCAGGAC CTGCAGACGA GGCTGAAGAA CCCGTAGAAG AGCCAGAGGA AGAGCATGCA 
GAAACTGGAG AAAGCAGTGC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_699475.5
CDS	145..3891
Translation	MMAGEQASGASSQIHSNEEDKAFDMTTSERVVDLLNQAALISTEEKLIVLKQVQELVINKDPSLLDNFLDEIIAFQTDKSIEVRKFVIGFIEEACKRDNELLLRLIANLNMLMRDESVNVVKKAILTLTQLYKVALQWLVRTKSVTDMQEACWDMVTQMKGDVLALLDSDNDGVRTHAIKFTESLIITLSPRTPDSDTPKKQEGDISLDKIPKDHTYIRYDTLCEEGKTALDQLLKFMVHPAISSINLTTALGSLATLARQRPMFMSEVVQAYEMLHANLPPTLAKSQVSSVRKNLKLHLVAVLKHPSSVDFHVQISTLLQDLGMSPSEITRCTPVAVQPRKRLHHEPYVEGKRIKMEAVEDDEDKEEPAPVVTPKPSSSIGTQSAIDLTAEFLLPLLTSDNVANLVLISMVYLPEVMPASFQATYTPVESAGTDAQIKHLARLMATQMTAAGLGPGLEQCKARDEEVKEEGAAGESSSKDPLIIRKLSAVSLGQAISVVGASAAYKSPIIEEAPQIKKLPEPILPSTQLKAPGASGRKKVFKLADVVQPFSEVQIEKLANMAIKRILYSEKAIAQTGMSHVRVKLLARLVTQFEGTKKNDLLQFVLEDMRSRSELAFSLLYHEYNEYLSQEPSGCLDSYEHCLFTLLSGLQERPEQRDGLFTKIVLEAPIITDSALEVIQRYCEDESRVYLGMSTLKELIFKRPSRQYQYLNVLLNLTSHEKEKVRSTALSFIKRMYEKEHLKDCIEKFALTYMQFLVHPNPPSLLFGAGEDTEVAAPWTEETVRQCLYLYLSLLPLNHRLVHELAAVYTEAIADIKRSVLRVIEQPIRGMGMNSPDLLLLVENCPKGAETLVTRCLHILTDKVPPSPELVERVRDLYHKRVPDVRFLIPVINGLEKNEVIQALPKLIKLNPIVVKEVFNRLLGTQHSEGSSSMSPLTPGELLIALHNIDSSKCDMKSIIKATNLCFGEKNVYTSEVLAVVMQQLMEQTPLPILLMRTVIQSLTMYPRLAGFVMNILARLILKQVWKYPKVWEGFVKCCQRTKPQSYNVLLQLPPPQLASVFERCPEMREPLLQHVHSFTPHQQAHIPTSIMTVLEATNRKPEPEPQPEIQHPEVMEEREVPLPKHISVHAPVQQAEPPAPKPIVFTEPMPPQEKHHHHHQVPSRREVAEVAEPMEQEETAHVPEEDTSEAPPQQMDVSEEAPQSSPVAEKADESMDESTAGPADEAEEPVEEPEEEHAETGESSAD

Target ORF information:

RefSeq Version	XM_694383.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio symplekin (sympk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_694383.7

ORF Insert Sequence:

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGCAGAT GGATGTGTCC 
GAGGAGGCTC CACAGTCCAG CCCGGTAGCA GAAAAGGCAG ATGAAAGCAT GGACGAGTCG 
ACTGCAGGAC CTGCAGACGA GGCTGAAGAA CCCGTAGAAG AGCCAGAGGA AGAGCATGCA 
GAAACTGGAG AAAGCAGTGC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sympk ( XM_005165573.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165630.1 Danio rerio symplekin (sympk), transcript variant X2, mRNA.

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CloneID	ODa11113
Clone ID Related Accession (Same CDS sequence)	XM_005165573.3 , XM_005165573.4
Accession Version	XM_005165573.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	symplekin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGATGGA TGTGTCCGAG 
GAGGCTCCAC AGTCCAGCCC GGTAGCAGAA AAGGCAGATG AAAGCATGGA CGAGTCGACT 
GCAGGACCTG CAGACGAGGC TGAAGAACCC GTAGAAGAGC CAGAGGAAGA GCATGCAGAA 
ACTGGAGAAA GCAGTGCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165630.1
CDS	146..3889
Translation	MMAGEQASGASSQIHSNEEDKAFDMTTSERVVDLLNQAALISTEEKLIVLKQVQELVINKDPSLLDNFLDEIIAFQTDKSIEVRKFVIGFIEEACKRDNELLLRLIANLNMLMRDESVNVVKKAILTLTQLYKVALQWLVRTKSVTDMQEACWDMVTQMKGDVLALLDSDNDGVRTHAIKFTESLIITLSPRTPDSDTPKKQEGDISLDKIPKDHTYIRYDTLCEEGKTALDQLLKFMVHPAISSINLTTALGSLATLARQRPMFMSEVVQAYEMLHANLPPTLAKSQVSSVRKNLKLHLVAVLKHPSSVDFHVQISTLLQDLGMSPSEITRCTPVAVQPRKRLHHEPYVEGKRIKMEAVEDDEDKEEPAPVVTPKPSSSIGTQSAIDLTAEFLLPLLTSDNVANLVLISMVYLPEVMPASFQATYTPVESAGTDAQIKHLARLMATQMTAAGLGPGLEQCKARDEEVKEEGAAGESSSKDPLIIRKLSAVSLGQAISVVGASAAYKSPIIEEAPQIKKLPEPILPSTQLKAPGASGRKKVFKLADVVQPFSEVQIEKLANMAIKRILYSEKAIAQTGMSHVRVKLLARLVTQFEGTKKNDLLQFVLEDMRSRSELAFSLLYHEYNEYLSQEPSGCLDSYEHCLFTLLSGLQERPEQRDGLFTKIVLEAPIITDSALEVIQRYCEDESRVYLGMSTLKELIFKRPSRQYQYLNVLLNLTSHEKEKVRSTALSFIKRMYEKEHLKDCIEKFALTYMQFLVHPNPPSLLFGAGEDTEVAAPWTEETVRQCLYLYLSLLPLNHRLVHELAAVYTEAIADIKRSVLRVIEQPIRGMGMNSPDLLLLVENCPKGAETLVTRCLHILTDKVPPSPELVERVRDLYHKRVPDVRFLIPVINGLEKNEVIQALPKLIKLNPIVVKEVFNRLLGTQHSEGSSSMSPLTPGELLIALHNIDSSKCDMKSIIKATNLCFGEKNVYTSEVLAVVMQQLMEQTPLPILLMRTVIQSLTMYPRLAGFVMNILARLILKQVWKYPKVWEGFVKCCQRTKPQSYNVLLQLPPPQLASVFERCPEMREPLLQHVHSFTPHQQAHIPTSIMTVLEATNRKPEPEPQPEIQHPEVMEEREVPLPKHISVHAPVQQAEPPAPKPIVFTEPMPPQEKHHHHHQVPSRREVAEVAEPMEQEETAHVPEEDTSEAPPQMDVSEEAPQSSPVAEKADESMDESTAGPADEAEEPVEEPEEEHAETGESSAD

Target ORF information:

RefSeq Version	XM_005165573.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio symplekin (sympk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165573.3

ORF Insert Sequence:

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGATGGA TGTGTCCGAG 
GAGGCTCCAC AGTCCAGCCC GGTAGCAGAA AAGGCAGATG AAAGCATGGA CGAGTCGACT 
GCAGGACCTG CAGACGAGGC TGAAGAACCC GTAGAAGAGC CAGAGGAAGA GCATGCAGAA 
ACTGGAGAAA GCAGTGCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sympk ( XM_005165573.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165630.1 Danio rerio symplekin (sympk), transcript variant X2, mRNA.

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CloneID	ODa11113
Clone ID Related Accession (Same CDS sequence)	XM_005165573.3 , XM_005165573.4
Accession Version	XM_005165573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	symplekin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165573.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGATGGA TGTGTCCGAG 
GAGGCTCCAC AGTCCAGCCC GGTAGCAGAA AAGGCAGATG AAAGCATGGA CGAGTCGACT 
GCAGGACCTG CAGACGAGGC TGAAGAACCC GTAGAAGAGC CAGAGGAAGA GCATGCAGAA 
ACTGGAGAAA GCAGTGCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165630.1
CDS	160..3903
Translation	MMAGEQASGASSQIHSNEEDKAFDMTTSERVVDLLNQAALISTEEKLIVLKQVQELVINKDPSLLDNFLDEIIAFQTDKSIEVRKFVIGFIEEACKRDNELLLRLIANLNMLMRDESVNVVKKAILTLTQLYKVALQWLVRTKSVTDMQEACWDMVTQMKGDVLALLDSDNDGVRTHAIKFTESLIITLSPRTPDSDTPKKQEGDISLDKIPKDHTYIRYDTLCEEGKTALDQLLKFMVHPAISSINLTTALGSLATLARQRPMFMSEVVQAYEMLHANLPPTLAKSQVSSVRKNLKLHLVAVLKHPSSVDFHVQISTLLQDLGMSPSEITRCTPVAVQPRKRLHHEPYVEGKRIKMEAVEDDEDKEEPAPVVTPKPSSSIGTQSAIDLTAEFLLPLLTSDNVANLVLISMVYLPEVMPASFQATYTPVESAGTDAQIKHLARLMATQMTAAGLGPGLEQCKARDEEVKEEGAAGESSSKDPLIIRKLSAVSLGQAISVVGASAAYKSPIIEEAPQIKKLPEPILPSTQLKAPGASGRKKVFKLADVVQPFSEVQIEKLANMAIKRILYSEKAIAQTGMSHVRVKLLARLVTQFEGTKKNDLLQFVLEDMRSRSELAFSLLYHEYNEYLSQEPSGCLDSYEHCLFTLLSGLQERPEQRDGLFTKIVLEAPIITDSALEVIQRYCEDESRVYLGMSTLKELIFKRPSRQYQYLNVLLNLTSHEKEKVRSTALSFIKRMYEKEHLKDCIEKFALTYMQFLVHPNPPSLLFGAGEDTEVAAPWTEETVRQCLYLYLSLLPLNHRLVHELAAVYTEAIADIKRSVLRVIEQPIRGMGMNSPDLLLLVENCPKGAETLVTRCLHILTDKVPPSPELVERVRDLYHKRVPDVRFLIPVINGLEKNEVIQALPKLIKLNPIVVKEVFNRLLGTQHSEGSSSMSPLTPGELLIALHNIDSSKCDMKSIIKATNLCFGEKNVYTSEVLAVVMQQLMEQTPLPILLMRTVIQSLTMYPRLAGFVMNILARLILKQVWKYPKVWEGFVKCCQRTKPQSYNVLLQLPPPQLASVFERCPEMREPLLQHVHSFTPHQQAHIPTSIMTVLEATNRKPEPEPQPEIQHPEVMEEREVPLPKHISVHAPVQQAEPPAPKPIVFTEPMPPQEKHHHHHQVPSRREVAEVAEPMEQEETAHVPEEDTSEAPPQMDVSEEAPQSSPVAEKADESMDESTAGPADEAEEPVEEPEEEHAETGESSAD

Target ORF information:

RefSeq Version	XM_005165573.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio symplekin (sympk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165573.4

ORF Insert Sequence:

ATGATGGCTG GAGAACAAGC TTCTGGTGCA TCTTCACAGA TCCACAGCAA TGAGGAAGAT 
AAAGCCTTTG ACATGACCAC CAGCGAAAGG GTGGTGGATC TGCTGAACCA GGCGGCTCTC 
ATCTCTACTG AAGAAAAGCT CATTGTTCTC AAACAGGTAC AGGAGTTGGT TATCAACAAA 
GACCCGTCGT TGCTTGATAA TTTCCTGGAT GAAATTATCG CATTTCAGAC GGATAAATCC 
ATTGAAGTGC GAAAGTTTGT GATTGGATTC ATTGAGGAGG CATGTAAAAG AGACAATGAG 
CTTCTGCTGC GGCTGATTGC CAACCTGAAC ATGCTGATGC GAGATGAGAG CGTGAACGTG 
GTGAAAAAGG CCATTCTGAC ACTCACTCAG CTCTACAAAG TGGCTTTACA GTGGCTGGTG 
CGCACTAAGA GTGTCACAGA CATGCAGGAG GCATGCTGGG ATATGGTAAC TCAAATGAAA 
GGGGATGTCC TGGCGTTGCT TGATTCAGAT AACGATGGAG TCCGCACTCA CGCCATCAAA 
TTCACAGAGT CCCTCATCAT CACTCTGTCA CCTCGAACGC CCGACTCTGA CACACCCAAG 
AAGCAGGAGG GCGACATAAG CCTGGATAAA ATCCCCAAAG ACCACACATA TATTCGATAC 
GACACATTAT GTGAGGAGGG TAAGACGGCT CTGGATCAGC TGTTAAAGTT CATGGTTCAC 
CCTGCCATCT CCAGCATTAA TCTCACCACT GCACTGGGAT CACTGGCCAC ACTGGCTAGA 
CAGCGGCCTA TGTTCATGTC CGAGGTGGTG CAGGCCTATG AGATGCTACA TGCTAACTTG 
CCTCCAACTC TGGCTAAGTC TCAAGTGAGC AGTGTCCGCA AGAACCTGAA GCTTCACCTG 
GTGGCAGTGC TGAAGCATCC CAGCAGTGTG GACTTCCATG TTCAGATCTC CACCCTGCTG 
CAGGACCTGG GCATGTCTCC GAGCGAAATC ACCCGCTGCA CGCCAGTCGC CGTACAGCCC 
CGTAAGAGAC TCCACCACGA GCCCTATGTG GAGGGCAAGA GGATCAAGAT GGAGGCTGTG 
GAGGATGATG AGGATAAGGA GGAACCGGCT CCAGTCGTCA CCCCAAAACC ATCCTCCTCC 
ATCGGCACCC AGTCAGCCAT TGATCTCACT GCTGAGTTCC TGTTACCTTT GCTGACATCA 
GACAATGTGG CCAACCTGGT GCTCATCAGT ATGGTTTACC TCCCTGAAGT GATGCCAGCC 
TCTTTCCAGG CCACTTACAC GCCAGTAGAA TCGGCTGGCA CTGATGCCCA GATTAAACAT 
TTAGCCAGGC TGATGGCCAC ACAGATGACT GCAGCTGGAC TGGGACCAGG GCTGGAGCAG 
TGTAAGGCTC GAGATGAGGA GGTGAAAGAA GAGGGAGCAG CAGGAGAGTC CAGCTCCAAA 
GACCCACTCA TTATCCGTAA GCTGTCTGCG GTGTCTCTCG GTCAAGCCAT CTCAGTGGTC 
GGAGCATCGG CAGCCTACAA GAGCCCCATC ATTGAAGAGG CTCCTCAGAT TAAGAAGCTT 
CCAGAGCCCA TCCTGCCCAG CACACAGCTC AAAGCCCCCG GTGCCAGTGG CAGAAAAAAA 
GTGTTCAAAT TGGCAGATGT GGTGCAGCCG TTTTCAGAAG TTCAGATTGA GAAACTCGCC 
AACATGGCCA TCAAGCGAAT CCTTTACTCT GAGAAGGCCA TTGCGCAGAC TGGGATGTCC 
CATGTCAGAG TGAAGCTGCT TGCACGGTTG GTGACCCAGT TTGAGGGTAC AAAGAAAAAC 
GATTTGCTGC AGTTTGTCCT GGAGGATATG CGGAGCAGAA GTGAGCTGGC CTTCTCTCTG 
TTGTACCACG AATACAATGA ATACTTGAGT CAGGAACCTT CAGGCTGTCT GGACAGCTAC 
GAGCACTGTC TGTTCACACT GCTGTCCGGC CTGCAGGAGA GACCTGAGCA GAGGGACGGG 
CTTTTCACCA AGATTGTGTT GGAGGCTCCG ATCATCACAG ATTCAGCACT GGAGGTCATT 
CAGCGATACT GTGAGGACGA GTCACGTGTT TACCTGGGCA TGTCCACATT AAAGGAGCTC 
ATCTTCAAAC GGCCCTCCAG ACAGTACCAG TACCTCAATG TCCTGCTGAA TCTCACCTCT 
CATGAAAAAG AAAAGGTGCG GTCGACTGCT CTTAGCTTCA TCAAGCGCAT GTACGAGAAG 
GAACATCTGA AGGACTGCAT TGAGAAATTC GCTCTCACCT ACATGCAGTT TCTGGTCCAT 
CCCAACCCCC CATCTCTTCT GTTCGGAGCT GGAGAAGATA CAGAGGTGGC AGCTCCCTGG 
ACGGAGGAGA CGGTGCGCCA GTGTCTTTAC CTCTACCTCT CCCTCCTGCC CCTCAACCAT 
CGTCTGGTGC ATGAACTGGC TGCCGTCTAC ACCGAGGCTA TCGCTGACAT CAAGCGCAGT 
GTGCTGCGGG TCATCGAGCA GCCTATAAGA GGAATGGGAA TGAACTCTCC CGACCTGCTG 
CTCCTGGTGG AGAACTGTCC TAAAGGAGCA GAAACACTGG TCACTCGCTG TCTGCACATA 
CTTACAGATA AAGTGCCGCC ATCTCCTGAA TTGGTGGAGA GGGTGAGGGA TCTTTATCAC 
AAACGAGTGC CAGATGTTCG TTTCCTCATC CCAGTCATCA ACGGTCTGGA GAAGAATGAA 
GTGATTCAGG CTCTGCCTAA ACTCATCAAG CTCAATCCTA TCGTGGTGAA GGAGGTGTTC 
AACAGACTGC TGGGCACTCA ACACAGTGAA GGCAGTTCCT CCATGTCTCC ACTCACTCCT 
GGAGAGCTGC TCATCGCCTT ACACAACATT GACTCCTCTA AGTGTGACAT GAAGTCAATC 
ATTAAAGCCA CCAACCTGTG TTTTGGGGAG AAGAACGTGT ACACGTCTGA GGTGCTGGCT 
GTGGTGATGC AGCAGCTGAT GGAGCAGACG CCTCTCCCCA TTCTGCTAAT GCGCACCGTC 
ATCCAGTCCC TCACCATGTA CCCTCGCCTG GCTGGCTTCG TCATGAACAT CTTAGCCCGA 
CTCATCCTCA AACAGGTGTG GAAGTATCCG AAAGTGTGGG AGGGTTTTGT TAAGTGCTGC 
CAGCGCACCA AACCCCAGTC CTACAACGTT CTCCTCCAGC TGCCGCCTCC ACAGCTGGCG 
AGTGTGTTCG AGCGCTGCCC GGAGATGAGA GAGCCTCTCC TGCAGCACGT CCACTCCTTC 
ACTCCTCACC AGCAAGCTCA CATTCCAACC TCCATAATGA CCGTCCTGGA AGCCACCAAC 
AGGAAACCAG AGCCTGAGCC ACAACCTGAA ATACAACATC CAGAAGTAAT GGAAGAGCGA 
GAGGTACCAT TACCAAAGCA TATATCAGTG CATGCACCAG TACAACAAGC AGAACCCCCA 
GCCCCAAAAC CCATAGTGTT TACTGAGCCA ATGCCACCAC AAGAAAAACA CCACCACCAC 
CACCAGGTCC CATCCAGACG GGAAGTAGCA GAAGTAGCAG AGCCCATGGA GCAGGAAGAG 
ACGGCCCATG TTCCAGAGGA GGACACATCT GAAGCACCAC CACAGATGGA TGTGTCCGAG 
GAGGCTCCAC AGTCCAGCCC GGTAGCAGAA AAGGCAGATG AAAGCATGGA CGAGTCGACT 
GCAGGACCTG CAGACGAGGC TGAAGAACCC GTAGAAGAGC CAGAGGAAGA GCATGCAGAA 
ACTGGAGAAA GCAGTGCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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sympk cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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