flnb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	flnb
Entrez Gene ID	563023
Full Name	filamin B, beta (actin binding protein 278)
Synonyms	si:ch211-222g5.4,si:ch211-222g5.6,si:ch211-222g5.7
General protein information	Preferred Names filamin B, beta (actin binding protein 278) Names filamin-A filamin-B filamin-C si:ch211-222g5.6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469934.1	XP_021325609.1	filamin-C isoform X2
XM_021474535.1	XP_021330210.1	filamin-A isoform X3
XM_021474536.1	XP_021330211.1	filamin-A isoform X4
XM_686387.7	XP_691479.5	filamin-A
XM_021469933.1	XP_021325608.1	filamin-A isoform X1
XM_021474537.1	XP_021330212.1	filamin-B isoform X5
XM_021474538.1	XP_021330213.1	filamin-B isoform X6

Pathways from BioSystems
Gene Ontology

The following flnb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flnb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146814	XM_021474538.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa151550	XM_021469934.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146811	XM_021474535.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa146812	XM_021474536.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa146813	XM_021474537.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa27643	XM_021469933.1 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ODa27643	XM_686387.7 Latest version!	Danio rerio filamin B, beta (actin binding protein 278) (flnb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

flnb ( XM_021474538.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330213.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X6, mRNA.

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CloneID	ODa146814
Clone ID Related Accession (Same CDS sequence)	XM_021474538.1
Accession Version	XM_021474538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA 
AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC 
ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC 
GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA 
GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA 
GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC 
AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG 
ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC 
ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT 
CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA 
ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC 
ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT 
GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA 
GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT 
TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT 
GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA 
GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT 
GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA 
ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT 
AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT 
GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA 
ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT 
GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG 
CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGTTT TTATAAACCC AATACAGATG 
GACATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330213.1
CDS	996..6224
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGREVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCLVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEMCYSDEELDLDEDFPENVHPGGRGELVMNVFSPSNTPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLAVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGESCIVNIDCAKVGSEDLSVQAVSDSGVVVETEVKENDDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCLNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPLLDPQSLYGSSSPNQFQALPKYGRDKELSSAHDLNMTDIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVQFPNEESFNRQFQVLATDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVSYTPAMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGSHSIMVKYTNDDCFRSIHRFNAIPPHSARKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRGEPEANAKANGSIKVADIMDNRDKKCEMPCTPKMSGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTTEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSETTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITVFINPIQMDI

Target ORF information:

RefSeq Version	XM_021474538.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474538.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA 
AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC 
ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC 
GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA 
GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA 
GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC 
AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG 
ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC 
ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT 
CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA 
ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC 
ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT 
GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA 
GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT 
TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT 
GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA 
GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT 
GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA 
ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT 
AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT 
GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA 
ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT 
GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG 
CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGTTT TTATAAACCC AATACAGATG 
GACATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_021469934.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325609.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X2, mRNA.

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CloneID	ODa151550
Clone ID Related Accession (Same CDS sequence)	XM_021469934.1
Accession Version	XM_021469934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGT TTTTATAAAC CCAATACAGA TGGACATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325609.1
CDS	559..5769
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGSEVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCQVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEICYSDEDFPENVHPGGRGELVMNVISPSNMPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLDVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGEPCIVNIDCAKVGSEDLSVQAISDSGVVVETEVKENEDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCVNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPVLDPQSLYGSSSPNQFQALPKYGRDKELSLAHDLNMADIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVKFPNEESFNRQFQVLPTDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVNYTPTMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGPHSIMAKYTNDDCFRSIHRFNAIPPHTVRKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRVEPEANAKANGSIKLADILDNRDKKCEMSCTPKMPGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTNEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSATTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITVFINPIQMDI

Target ORF information:

RefSeq Version	XM_021469934.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469934.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGT TTTTATAAAC CCAATACAGA TGGACATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_021474535.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330210.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa146811
Clone ID Related Accession (Same CDS sequence)	XM_021474535.1
Accession Version	XM_021474535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA 
AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC 
ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC 
GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA 
GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA 
GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC 
AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG 
ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC 
ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT 
CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA 
ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC 
ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT 
GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA 
GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT 
TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT 
GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA 
GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT 
GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA 
ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT 
AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT 
GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA 
ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT 
GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG 
CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGATG GACATATGAG AAAGTCAGAG 
GTCAAACTGG GCAGTGCGGT CGATTTTACC CTGGACATCA CAGAAGAAGA CATTAGCCAG 
CTCTCCGCTA GCATTATGTC GCCCACAGGC AATGATGTAC CCTGCTTACT GAAAACACAA 
GCTGACAGCC ATCTCGGCGT CTCCTTCATC CCCAGAGAGG CTGGTGAGCA TCTGGTTAGC 
ATCATGAAGG ATGGGGAGCA TGTGAGCAAC AGTCCCATCT CTCTGTCCAT CAGTCAGGCA 
GAGATTGGAG ATGCCAGTAA GGTGAAGGCT TTCGGTCCAG GCTTACACAC CGGGCACACC 
TTCTGCAAGT CTGAATTTGT GGTGGACACT TGGGATGCAG GATATGGTGG GTTAACAGTT 
GTAATTGAAG GTCCCAGTAA AGTAGACGTC CACACAGAAG AGCTGGAGAA TGATGGAACT 
TGCAATATAT CATACTGCCC AATCAAGCCT GGCAGCTATA AAATCTCCAT CAAATTCTCA 
GATGAGCACA TTCCAGGGAG TCCGTTTACA GCTGTGGTGA AAGACCAAGG GCTTATGAGA 
GAGAACATCA CATACAACCA GAAAGCTGCT CCTACTGCCA GCATTGGGAG TGAATGCAGT 
TTAGCCTTCA AACTCCCAGA TGTTGATCCT GGGACTATGT CTGCTCATGT ATATGAACCA 
TCAGGAAGTT TTGGAGAGGC TGTTATCCTT CCTAGAACAG GCCGTGACAT CTATGTAGTG 
AGCTTTCTAG CTAAAGAGAT GGGAGTTTAT AATGTGAGCA TACAGAACAA AGGCCAGACG 
ATTTCCGGCT GCCCACTTCA ATACACCGTC GGCCCGCTGG GACGAGGAGG ATCTGAGAAG 
GTGCAGGTTT GGGGACAAAG CCTTCAGACA GCTCTGGCAA CTGTTCCAGC TGACTTTAAT 
ATTTGGTCGC GTGAGGCTGG AGCAGGGGCT CTGTCTGTCT CCGTAGAGGG TCCTGGAGAG 
ATTGAGCTCC ACTTTGATGA GCAGATGGAC GGGTCTTGCA TTGTGACCTA TACTGCACAA 
CAGCCTGGTG ACTATGAGGT TTCAGTCATG TTTAATGAGG ATCATGTTTC CCAAAGCCCT 
TTTCTTGTGT CTGTCATTGC CGCCACAGAC ACTGCTGTCT CTGATCAAAC TCAGCTGTGT 
AACGGTCATG GAGAAGCCTC TCTGTCAGGA CCGCAGATCA ACACAACATC TGACAGCAGT 
GCTGAGCCAG TTTTGCTGTC TGACGCTAGT AAAGTCATCT GCCACGGCCC AGGCCTGAGT 
AAGGGCTTTC TGGGACGCAA AAACACTTTT TATGTGGACT GCAGTAAAGC TGGACGAAAC 
CTTCTGTTCG TGGGCATGCA TGGCCCTACA GTTCCCTGTG AGCGGGTGTC CATCATTCAC 
ATGGGCCAGT GTCAGTACAG GATCAACTAC GCTGTAAAGG AAAGAGGAAA ATATGTCTTA 
GCAGTGAAGT GGGGAGATGA GCACATTCCT GGATCGCCGT TTTACATTAG TGTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330210.1
CDS	996..7715
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGREVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCLVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEMCYSDEELDLDEDFPENVHPGGRGELVMNVFSPSNTPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLAVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGESCIVNIDCAKVGSEDLSVQAVSDSGVVVETEVKENDDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCLNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPLLDPQSLYGSSSPNQFQALPKYGRDKELSSAHDLNMTDIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVQFPNEESFNRQFQVLATDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVSYTPAMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGSHSIMVKYTNDDCFRSIHRFNAIPPHSARKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRGEPEANAKANGSIKVADIMDNRDKKCEMPCTPKMSGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTTEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSETTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITDGHMRKSEVKLGSAVDFTLDITEEDISQLSASIMSPTGNDVPCLLKTQADSHLGVSFIPREAGEHLVSIMKDGEHVSNSPISLSISQAEIGDASKVKAFGPGLHTGHTFCKSEFVVDTWDAGYGGLTVVIEGPSKVDVHTEELENDGTCNISYCPIKPGSYKISIKFSDEHIPGSPFTAVVKDQGLMRENITYNQKAAPTASIGSECSLAFKLPDVDPGTMSAHVYEPSGSFGEAVILPRTGRDIYVVSFLAKEMGVYNVSIQNKGQTISGCPLQYTVGPLGRGGSEKVQVWGQSLQTALATVPADFNIWSREAGAGALSVSVEGPGEIELHFDEQMDGSCIVTYTAQQPGDYEVSVMFNEDHVSQSPFLVSVIAATDTAVSDQTQLCNGHGEASLSGPQINTTSDSSAEPVLLSDASKVICHGPGLSKGFLGRKNTFYVDCSKAGRNLLFVGMHGPTVPCERVSIIHMGQCQYRINYAVKERGKYVLAVKWGDEHIPGSPFYISVV

Target ORF information:

RefSeq Version	XM_021474535.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474535.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGTATTCACA GGTTTAATGC CATTCCTCCT CACTCTGCCA GAAAGGATAA AGACATTGGA 
AATAAAAAGG AAAATTCGAG TCAGGAGGGA AATGTCCACT CTAAGCCTCC ACATACATTC 
ACTAACGGCT GCCGTGGGGA ACCAGAAGCT AATGCTAAAG CTAATGGCTC AATAAAAGTC 
GCAGATATAA TGGACAACAG GGATAAAAAA TGTGAGATGC CTTGCACGCC AAAAATGTCA 
GGCTGTAAGT CTGTAATGGA CAAATACAAC AGCAAAGAAG CTTTCAGAAG CCTCCAGACA 
GATCCGACTG TAAATACAGA GCAATCAGTC TGTGCTCAAA GTCCTCAGTT GTTCAGGATC 
AGCTGTAGTA AATCCGGGAA GCCCCCTGAC TGTGTGCTGA TGATCAGACC TGATGGAGAG 
ACTGAACTGA CAGAGGTCAA AGAAAACCCT GACGGGACTT ACTCTGTCAA ATACTGTCCC 
ACTAATGAAG GTCTTCATTC CTTGATGGTC AAATATGTTG ATGATGATTC ATTTTACAAT 
CCTTTGAGGT TTCATGTCCT TCCTCACGCT GATAGTCATG ACAAACATGT GATTAATACA 
ACTGAAGCTT GTAGCAGACA ATTAGAGGAA CGTGTCAGCG CTCAAATTCC TCAGACTTTC 
ACAACTTGCT GCAACACTAA CGAGGCCTCA GGGACCGAGA TGTTGAAAGC TGATGATCAT 
GTTGAAGTGT TAAATAATGA GAACACAACC AGTGAAACAA CACACACACC TGAACCCTCA 
GCGGTCAGAA ATGCCACACT GGAGGATTTT CAAAGGCACC AGCCACCATC CTCTCACACT 
TCAAGTGGGT ACAGGGGTTT TCAAAACAAG GTTGAGGGCA GGAATGCAAT CCCTCCTGCT 
GCCATTAATC AAGCAGGGTT TATATCCTGT GATATAGTCC TGCCAGGAGA CTTAAGTGAA 
GGTGAAATTA CAGGAGAGGT GATCACGCCG TCTGGAAAGA GAGCACAGCC TGACATGATT 
GATAATAAAG ATGGGACTGT CGTTATGAAG TTTGATCCTT CAGAGGAGGG TCTTCATCAA 
ATCCTTATTA AATCAGCAGA AAATGATATA CCAGAACTTC CACTGCAATA TTATGCAAAT 
AGTTTGGCTA ATCGCAGCCC TGTGGCCTAT GGGCGTGGCC TTGTGTGTGG AATTGCAAAT 
GAGACTGCAA CATTCACCAT CTGTCAGGAG GATTCAGCCT CGAGTGAACT GGACATCACA 
ATTGAGGGTC CGTCTGAGGC TGATGTGCAC TGCTTGGACA ATGAAGATGG CACTTGTTCT 
GTGAGTTACC TTCCAACTGA GCCTGGAGAT TATGAGATAC AAGTTCAACA TAATGACGTG 
CCTATTCCAG GCAGTCCTTT CAAGGCCAAA ATTACAGATG GACATATGAG AAAGTCAGAG 
GTCAAACTGG GCAGTGCGGT CGATTTTACC CTGGACATCA CAGAAGAAGA CATTAGCCAG 
CTCTCCGCTA GCATTATGTC GCCCACAGGC AATGATGTAC CCTGCTTACT GAAAACACAA 
GCTGACAGCC ATCTCGGCGT CTCCTTCATC CCCAGAGAGG CTGGTGAGCA TCTGGTTAGC 
ATCATGAAGG ATGGGGAGCA TGTGAGCAAC AGTCCCATCT CTCTGTCCAT CAGTCAGGCA 
GAGATTGGAG ATGCCAGTAA GGTGAAGGCT TTCGGTCCAG GCTTACACAC CGGGCACACC 
TTCTGCAAGT CTGAATTTGT GGTGGACACT TGGGATGCAG GATATGGTGG GTTAACAGTT 
GTAATTGAAG GTCCCAGTAA AGTAGACGTC CACACAGAAG AGCTGGAGAA TGATGGAACT 
TGCAATATAT CATACTGCCC AATCAAGCCT GGCAGCTATA AAATCTCCAT CAAATTCTCA 
GATGAGCACA TTCCAGGGAG TCCGTTTACA GCTGTGGTGA AAGACCAAGG GCTTATGAGA 
GAGAACATCA CATACAACCA GAAAGCTGCT CCTACTGCCA GCATTGGGAG TGAATGCAGT 
TTAGCCTTCA AACTCCCAGA TGTTGATCCT GGGACTATGT CTGCTCATGT ATATGAACCA 
TCAGGAAGTT TTGGAGAGGC TGTTATCCTT CCTAGAACAG GCCGTGACAT CTATGTAGTG 
AGCTTTCTAG CTAAAGAGAT GGGAGTTTAT AATGTGAGCA TACAGAACAA AGGCCAGACG 
ATTTCCGGCT GCCCACTTCA ATACACCGTC GGCCCGCTGG GACGAGGAGG ATCTGAGAAG 
GTGCAGGTTT GGGGACAAAG CCTTCAGACA GCTCTGGCAA CTGTTCCAGC TGACTTTAAT 
ATTTGGTCGC GTGAGGCTGG AGCAGGGGCT CTGTCTGTCT CCGTAGAGGG TCCTGGAGAG 
ATTGAGCTCC ACTTTGATGA GCAGATGGAC GGGTCTTGCA TTGTGACCTA TACTGCACAA 
CAGCCTGGTG ACTATGAGGT TTCAGTCATG TTTAATGAGG ATCATGTTTC CCAAAGCCCT 
TTTCTTGTGT CTGTCATTGC CGCCACAGAC ACTGCTGTCT CTGATCAAAC TCAGCTGTGT 
AACGGTCATG GAGAAGCCTC TCTGTCAGGA CCGCAGATCA ACACAACATC TGACAGCAGT 
GCTGAGCCAG TTTTGCTGTC TGACGCTAGT AAAGTCATCT GCCACGGCCC AGGCCTGAGT 
AAGGGCTTTC TGGGACGCAA AAACACTTTT TATGTGGACT GCAGTAAAGC TGGACGAAAC 
CTTCTGTTCG TGGGCATGCA TGGCCCTACA GTTCCCTGTG AGCGGGTGTC CATCATTCAC 
ATGGGCCAGT GTCAGTACAG GATCAACTAC GCTGTAAAGG AAAGAGGAAA ATATGTCTTA 
GCAGTGAAGT GGGGAGATGA GCACATTCCT GGATCGCCGT TTTACATTAG TGTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_021474536.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330211.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X4, mRNA.

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CloneID	ODa146812
Clone ID Related Accession (Same CDS sequence)	XM_021474536.1
Accession Version	XM_021474536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGGTTTAATG CCATTCCTCC TCACTCTGCC AGAAAGGATA AAGACATTGG AAATAAAAAG 
GAAAATTCGA GTCAGGAGGG AAATGTCCAC TCTAAGCCTC CACATACATT CACTAACGGC 
TGCCGTGGGG AACCAGAAGC TAATGCTAAA GCTAATGGCT CAATAAAAGT CGCAGATATA 
ATGGACAACA GGGATAAAAA ATGTGAGATG CCTTGCACGC CAAAAATGTC AGGCTGTAAG 
TCTGTAATGG ACAAATACAA CAGCAAAGAA GCTTTCAGAA GCCTCCAGAC AGATCCGACT 
GTAAATACAG AGCAATCAGT CTGTGCTCAA AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT 
AAATCCGGGA AGCCCCCTGA CTGTGTGCTG ATGATCAGAC CTGATGGAGA GACTGAACTG 
ACAGAGGTCA AAGAAAACCC TGACGGGACT TACTCTGTCA AATACTGTCC CACTAATGAA 
GGTCTTCATT CCTTGATGGT CAAATATGTT GATGATGATT CATTTTACAA TCCTTTGAGG 
TTTCATGTCC TTCCTCACGC TGATAGTCAT GACAAACATG TGATTAATAC AACTGAAGCT 
TGTAGCAGAC AATTAGAGGA ACGTGTCAGC GCTCAAATTC CTCAGACTTT CACAACTTGC 
TGCAACACTA ACGAGGCCTC AGGGACCGAG ATGTTGAAAG CTGATGATCA TGTTGAAGTG 
TTAAATAATG AGAACACAAC CAGTGAAACA ACACACACAC CTGAACCCTC AGCGGTCAGA 
AATGCCACAC TGGAGGATTT TCAAAGGCAC CAGCCACCAT CCTCTCACAC TTCAAGTGGG 
TACAGGGGTT TTCAAAACAA GGTTGAGGGC AGGAATGCAA TCCCTCCTGC TGCCATTAAT 
CAAGCAGGGT TTATATCCTG TGATATAGTC CTGCCAGGAG ACTTAAGTGA AGGTGAAATT 
ACAGGAGAGG TGATCACGCC GTCTGGAAAG AGAGCACAGC CTGACATGAT TGATAATAAA 
GATGGGACTG TCGTTATGAA GTTTGATCCT TCAGAGGAGG GTCTTCATCA AATCCTTATT 
AAATCAGCAG AAAATGATAT ACCAGAACTT CCACTGCAAT ATTATGCAAA TAGTTTGGCT 
AATCGCAGCC CTGTGGCCTA TGGGCGTGGC CTTGTGTGTG GAATTGCAAA TGAGACTGCA 
ACATTCACCA TCTGTCAGGA GGATTCAGCC TCGAGTGAAC TGGACATCAC AATTGAGGGT 
CCGTCTGAGG CTGATGTGCA CTGCTTGGAC AATGAAGATG GCACTTGTTC TGTGAGTTAC 
CTTCCAACTG AGCCTGGAGA TTATGAGATA CAAGTTCAAC ATAATGACGT GCCTATTCCA 
GGCAGTCCTT TCAAGGCCAA AATTACAGAT GGACATATGA GAAAGTCAGA GGTCAAACTG 
GGCAGTGCGG TCGATTTTAC CCTGGACATC ACAGAAGAAG ACATTAGCCA GCTCTCCGCT 
AGCATTATGT CGCCCACAGG CAATGATGTA CCCTGCTTAC TGAAAACACA AGCTGACAGC 
CATCTCGGCG TCTCCTTCAT CCCCAGAGAG GCTGGTGAGC ATCTGGTTAG CATCATGAAG 
GATGGGGAGC ATGTGAGCAA CAGTCCCATC TCTCTGTCCA TCAGTCAGGC AGAGATTGGA 
GATGCCAGTA AGGTGAAGGC TTTCGGTCCA GGCTTACACA CCGGGCACAC CTTCTGCAAG 
TCTGAATTTG TGGTGGACAC TTGGGATGCA GGATATGGTG GGTTAACAGT TGTAATTGAA 
GGTCCCAGTA AAGTAGACGT CCACACAGAA GAGCTGGAGA ATGATGGAAC TTGCAATATA 
TCATACTGCC CAATCAAGCC TGGCAGCTAT AAAATCTCCA TCAAATTCTC AGATGAGCAC 
ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG AAAGACCAAG GGCTTATGAG AGAGAACATC 
ACATACAACC AGAAAGCTGC TCCTACTGCC AGCATTGGGA GTGAATGCAG TTTAGCCTTC 
AAACTCCCAG ATGTTGATCC TGGGACTATG TCTGCTCATG TATATGAACC ATCAGGAAGT 
TTTGGAGAGG CTGTTATCCT TCCTAGAACA GGCCGTGACA TCTATGTAGT GAGCTTTCTA 
GCTAAAGAGA TGGGAGTTTA TAATGTGAGC ATACAGAACA AAGGCCAGAC GATTTCCGGC 
TGCCCACTTC AATACACCGT CGGCCCGCTG GGACGAGGAG GATCTGAGAA GGTGCAGGTT 
TGGGGACAAA GCCTTCAGAC AGCTCTGGCA ACTGTTCCAG CTGACTTTAA TATTTGGTCG 
CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC TCCGTAGAGG GTCCTGGAGA GATTGAGCTC 
CACTTTGATG AGCAGATGGA CGGGTCTTGC ATTGTGACCT ATACTGCACA ACAGCCTGGT 
GACTATGAGG TTTCAGTCAT GTTTAATGAG GATCATGTTT CCCAAAGCCC TTTTCTTGTG 
TCTGTCATTG CCGCCACAGA CACTGCTGTC TCTGATCAAA CTCAGCTGTG TAACGGTCAT 
GGAGAAGCCT CTCTGTCAGG ACCGCAGATC AACACAACAT CTGACAGCAG TGCTGAGCCA 
GTTTTGCTGT CTGACGCTAG TAAAGTCATC TGCCACGGCC CAGGCCTGAG TAAGGGCTTT 
CTGGGACGCA AAAACACTTT TTATGTGGAC TGCAGTAAAG CTGGACGAAA CCTTCTGTTC 
GTGGGCATGC ATGGCCCTAC AGTTCCCTGT GAGCGGGTGT CCATCATTCA CATGGGCCAG 
TGTCAGTACA GGATCAACTA CGCTGTAAAG GAAAGAGGAA AATATGTCTT AGCAGTGAAG 
TGGGGAGATG AGCACATTCC TGGATCGCCG TTTTACATTA GTGTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330211.1
CDS	996..7706
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGREVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCLVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEMCYSDEELDLDEDFPENVHPGGRGELVMNVFSPSNTPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLAVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGESCIVNIDCAKVGSEDLSVQAVSDSGVVVETEVKENDDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCLNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPLLDPQSLYGSSSPNQFQALPKYGRDKELSSAHDLNMTDIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVQFPNEESFNRQFQVLATDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVSYTPAMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGSHSIMVKYTNDDCFRRFNAIPPHSARKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRGEPEANAKANGSIKVADIMDNRDKKCEMPCTPKMSGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTTEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSETTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITDGHMRKSEVKLGSAVDFTLDITEEDISQLSASIMSPTGNDVPCLLKTQADSHLGVSFIPREAGEHLVSIMKDGEHVSNSPISLSISQAEIGDASKVKAFGPGLHTGHTFCKSEFVVDTWDAGYGGLTVVIEGPSKVDVHTEELENDGTCNISYCPIKPGSYKISIKFSDEHIPGSPFTAVVKDQGLMRENITYNQKAAPTASIGSECSLAFKLPDVDPGTMSAHVYEPSGSFGEAVILPRTGRDIYVVSFLAKEMGVYNVSIQNKGQTISGCPLQYTVGPLGRGGSEKVQVWGQSLQTALATVPADFNIWSREAGAGALSVSVEGPGEIELHFDEQMDGSCIVTYTAQQPGDYEVSVMFNEDHVSQSPFLVSVIAATDTAVSDQTQLCNGHGEASLSGPQINTTSDSSAEPVLLSDASKVICHGPGLSKGFLGRKNTFYVDCSKAGRNLLFVGMHGPTVPCERVSIIHMGQCQYRINYAVKERGKYVLAVKWGDEHIPGSPFYISVV

Target ORF information:

RefSeq Version	XM_021474536.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474536.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGGTTTAATG CCATTCCTCC TCACTCTGCC AGAAAGGATA AAGACATTGG AAATAAAAAG 
GAAAATTCGA GTCAGGAGGG AAATGTCCAC TCTAAGCCTC CACATACATT CACTAACGGC 
TGCCGTGGGG AACCAGAAGC TAATGCTAAA GCTAATGGCT CAATAAAAGT CGCAGATATA 
ATGGACAACA GGGATAAAAA ATGTGAGATG CCTTGCACGC CAAAAATGTC AGGCTGTAAG 
TCTGTAATGG ACAAATACAA CAGCAAAGAA GCTTTCAGAA GCCTCCAGAC AGATCCGACT 
GTAAATACAG AGCAATCAGT CTGTGCTCAA AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT 
AAATCCGGGA AGCCCCCTGA CTGTGTGCTG ATGATCAGAC CTGATGGAGA GACTGAACTG 
ACAGAGGTCA AAGAAAACCC TGACGGGACT TACTCTGTCA AATACTGTCC CACTAATGAA 
GGTCTTCATT CCTTGATGGT CAAATATGTT GATGATGATT CATTTTACAA TCCTTTGAGG 
TTTCATGTCC TTCCTCACGC TGATAGTCAT GACAAACATG TGATTAATAC AACTGAAGCT 
TGTAGCAGAC AATTAGAGGA ACGTGTCAGC GCTCAAATTC CTCAGACTTT CACAACTTGC 
TGCAACACTA ACGAGGCCTC AGGGACCGAG ATGTTGAAAG CTGATGATCA TGTTGAAGTG 
TTAAATAATG AGAACACAAC CAGTGAAACA ACACACACAC CTGAACCCTC AGCGGTCAGA 
AATGCCACAC TGGAGGATTT TCAAAGGCAC CAGCCACCAT CCTCTCACAC TTCAAGTGGG 
TACAGGGGTT TTCAAAACAA GGTTGAGGGC AGGAATGCAA TCCCTCCTGC TGCCATTAAT 
CAAGCAGGGT TTATATCCTG TGATATAGTC CTGCCAGGAG ACTTAAGTGA AGGTGAAATT 
ACAGGAGAGG TGATCACGCC GTCTGGAAAG AGAGCACAGC CTGACATGAT TGATAATAAA 
GATGGGACTG TCGTTATGAA GTTTGATCCT TCAGAGGAGG GTCTTCATCA AATCCTTATT 
AAATCAGCAG AAAATGATAT ACCAGAACTT CCACTGCAAT ATTATGCAAA TAGTTTGGCT 
AATCGCAGCC CTGTGGCCTA TGGGCGTGGC CTTGTGTGTG GAATTGCAAA TGAGACTGCA 
ACATTCACCA TCTGTCAGGA GGATTCAGCC TCGAGTGAAC TGGACATCAC AATTGAGGGT 
CCGTCTGAGG CTGATGTGCA CTGCTTGGAC AATGAAGATG GCACTTGTTC TGTGAGTTAC 
CTTCCAACTG AGCCTGGAGA TTATGAGATA CAAGTTCAAC ATAATGACGT GCCTATTCCA 
GGCAGTCCTT TCAAGGCCAA AATTACAGAT GGACATATGA GAAAGTCAGA GGTCAAACTG 
GGCAGTGCGG TCGATTTTAC CCTGGACATC ACAGAAGAAG ACATTAGCCA GCTCTCCGCT 
AGCATTATGT CGCCCACAGG CAATGATGTA CCCTGCTTAC TGAAAACACA AGCTGACAGC 
CATCTCGGCG TCTCCTTCAT CCCCAGAGAG GCTGGTGAGC ATCTGGTTAG CATCATGAAG 
GATGGGGAGC ATGTGAGCAA CAGTCCCATC TCTCTGTCCA TCAGTCAGGC AGAGATTGGA 
GATGCCAGTA AGGTGAAGGC TTTCGGTCCA GGCTTACACA CCGGGCACAC CTTCTGCAAG 
TCTGAATTTG TGGTGGACAC TTGGGATGCA GGATATGGTG GGTTAACAGT TGTAATTGAA 
GGTCCCAGTA AAGTAGACGT CCACACAGAA GAGCTGGAGA ATGATGGAAC TTGCAATATA 
TCATACTGCC CAATCAAGCC TGGCAGCTAT AAAATCTCCA TCAAATTCTC AGATGAGCAC 
ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG AAAGACCAAG GGCTTATGAG AGAGAACATC 
ACATACAACC AGAAAGCTGC TCCTACTGCC AGCATTGGGA GTGAATGCAG TTTAGCCTTC 
AAACTCCCAG ATGTTGATCC TGGGACTATG TCTGCTCATG TATATGAACC ATCAGGAAGT 
TTTGGAGAGG CTGTTATCCT TCCTAGAACA GGCCGTGACA TCTATGTAGT GAGCTTTCTA 
GCTAAAGAGA TGGGAGTTTA TAATGTGAGC ATACAGAACA AAGGCCAGAC GATTTCCGGC 
TGCCCACTTC AATACACCGT CGGCCCGCTG GGACGAGGAG GATCTGAGAA GGTGCAGGTT 
TGGGGACAAA GCCTTCAGAC AGCTCTGGCA ACTGTTCCAG CTGACTTTAA TATTTGGTCG 
CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC TCCGTAGAGG GTCCTGGAGA GATTGAGCTC 
CACTTTGATG AGCAGATGGA CGGGTCTTGC ATTGTGACCT ATACTGCACA ACAGCCTGGT 
GACTATGAGG TTTCAGTCAT GTTTAATGAG GATCATGTTT CCCAAAGCCC TTTTCTTGTG 
TCTGTCATTG CCGCCACAGA CACTGCTGTC TCTGATCAAA CTCAGCTGTG TAACGGTCAT 
GGAGAAGCCT CTCTGTCAGG ACCGCAGATC AACACAACAT CTGACAGCAG TGCTGAGCCA 
GTTTTGCTGT CTGACGCTAG TAAAGTCATC TGCCACGGCC CAGGCCTGAG TAAGGGCTTT 
CTGGGACGCA AAAACACTTT TTATGTGGAC TGCAGTAAAG CTGGACGAAA CCTTCTGTTC 
GTGGGCATGC ATGGCCCTAC AGTTCCCTGT GAGCGGGTGT CCATCATTCA CATGGGCCAG 
TGTCAGTACA GGATCAACTA CGCTGTAAAG GAAAGAGGAA AATATGTCTT AGCAGTGAAG 
TGGGGAGATG AGCACATTCC TGGATCGCCG TTTTACATTA GTGTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_021474537.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330212.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X5, mRNA.

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CloneID	ODa146813
Clone ID Related Accession (Same CDS sequence)	XM_021474537.1
Accession Version	XM_021474537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGAAAGGATA AAGACATTGG AAATAAAAAG GAAAATTCGA GTCAGGAGGG AAATGTCCAC 
TCTAAGCCTC CACATACATT CACTAACGGC TGCCGTGGGG AACCAGAAGC TAATGCTAAA 
GCTAATGGCT CAATAAAAGT CGCAGATATA ATGGACAACA GGGATAAAAA ATGTGAGATG 
CCTTGCACGC CAAAAATGTC AGGCTGTAAG TCTGTAATGG ACAAATACAA CAGCAAAGAA 
GCTTTCAGAA GCCTCCAGAC AGATCCGACT GTAAATACAG AGCAATCAGT CTGTGCTCAA 
AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT AAATCCGGGA AGCCCCCTGA CTGTGTGCTG 
ATGATCAGAC CTGATGGAGA GACTGAACTG ACAGAGGTCA AAGAAAACCC TGACGGGACT 
TACTCTGTCA AATACTGTCC CACTAATGAA GGTCTTCATT CCTTGATGGT CAAATATGTT 
GATGATGATT CATTTTACAA TCCTTTGAGG TTTCATGTCC TTCCTCACGC TGATAGTCAT 
GACAAACATG TGATTAATAC AACTGAAGCT TGTAGCAGAC AATTAGAGGA ACGTGTCAGC 
GCTCAAATTC CTCAGACTTT CACAACTTGC TGCAACACTA ACGAGGCCTC AGGGACCGAG 
ATGTTGAAAG CTGATGATCA TGTTGAAGTG TTAAATAATG AGAACACAAC CAGTGAAACA 
ACACACACAC CTGAACCCTC AGCGGTCAGA AATGCCACAC TGGAGGATTT TCAAAGGCAC 
CAGCCACCAT CCTCTCACAC TTCAAGTGGG TACAGGGGTT TTCAAAACAA GGTTGAGGGC 
AGGAATGCAA TCCCTCCTGC TGCCATTAAT CAAGCAGGGT TTATATCCTG TGATATAGTC 
CTGCCAGGAG ACTTAAGTGA AGGTGAAATT ACAGGAGAGG TGATCACGCC GTCTGGAAAG 
AGAGCACAGC CTGACATGAT TGATAATAAA GATGGGACTG TCGTTATGAA GTTTGATCCT 
TCAGAGGAGG GTCTTCATCA AATCCTTATT AAATCAGCAG AAAATGATAT ACCAGAACTT 
CCACTGCAAT ATTATGCAAA TAGTTTGGCT AATCGCAGCC CTGTGGCCTA TGGGCGTGGC 
CTTGTGTGTG GAATTGCAAA TGAGACTGCA ACATTCACCA TCTGTCAGGA GGATTCAGCC 
TCGAGTGAAC TGGACATCAC AATTGAGGGT CCGTCTGAGG CTGATGTGCA CTGCTTGGAC 
AATGAAGATG GCACTTGTTC TGTGAGTTAC CTTCCAACTG AGCCTGGAGA TTATGAGATA 
CAAGTTCAAC ATAATGACGT GCCTATTCCA GGCAGTCCTT TCAAGGCCAA AATTACAGAT 
GGACATATGA GAAAGTCAGA GGTCAAACTG GGCAGTGCGG TCGATTTTAC CCTGGACATC 
ACAGAAGAAG ACATTAGCCA GCTCTCCGCT AGCATTATGT CGCCCACAGG CAATGATGTA 
CCCTGCTTAC TGAAAACACA AGCTGACAGC CATCTCGGCG TCTCCTTCAT CCCCAGAGAG 
GCTGGTGAGC ATCTGGTTAG CATCATGAAG GATGGGGAGC ATGTGAGCAA CAGTCCCATC 
TCTCTGTCCA TCAGTCAGGC AGAGATTGGA GATGCCAGTA AGGTGAAGGC TTTCGGTCCA 
GGCTTACACA CCGGGCACAC CTTCTGCAAG TCTGAATTTG TGGTGGACAC TTGGGATGCA 
GGATATGGTG GGTTAACAGT TGTAATTGAA GGTCCCAGTA AAGTAGACGT CCACACAGAA 
GAGCTGGAGA ATGATGGAAC TTGCAATATA TCATACTGCC CAATCAAGCC TGGCAGCTAT 
AAAATCTCCA TCAAATTCTC AGATGAGCAC ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG 
AAAGACCAAG GGCTTATGAG AGAGAACATC ACATACAACC AGAAAGCTGC TCCTACTGCC 
AGCATTGGGA GTGAATGCAG TTTAGCCTTC AAACTCCCAG ATGTTGATCC TGGGACTATG 
TCTGCTCATG TATATGAACC ATCAGGAAGT TTTGGAGAGG CTGTTATCCT TCCTAGAACA 
GGCCGTGACA TCTATGTAGT GAGCTTTCTA GCTAAAGAGA TGGGAGTTTA TAATGTGAGC 
ATACAGAACA AAGGCCAGAC GATTTCCGGC TGCCCACTTC AATACACCGT CGGCCCGCTG 
GGACGAGGAG GATCTGAGAA GGTGCAGGTT TGGGGACAAA GCCTTCAGAC AGCTCTGGCA 
ACTGTTCCAG CTGACTTTAA TATTTGGTCG CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC 
TCCGTAGAGG GTCCTGGAGA GATTGAGCTC CACTTTGATG AGCAGATGGA CGGGTCTTGC 
ATTGTGACCT ATACTGCACA ACAGCCTGGT GACTATGAGG TTTCAGTCAT GTTTAATGAG 
GATCATGTTT CCCAAAGCCC TTTTCTTGTG TCTGTCATTG CCGCCACAGA CACTGCTGTC 
TCTGATCAAA CTCAGCTGTG TAACGGTCAT GGAGAAGCCT CTCTGTCAGG ACCGCAGATC 
AACACAACAT CTGACAGCAG TGCTGAGCCA GTTTTGCTGT CTGACGCTAG TAAAGTCATC 
TGCCACGGCC CAGGCCTGAG TAAGGGCTTT CTGGGACGCA AAAACACTTT TTATGTGGAC 
TGCAGTAAAG CTGGACGAAA CCTTCTGTTC GTGGGCATGC ATGGCCCTAC AGTTCCCTGT 
GAGCGGGTGT CCATCATTCA CATGGGCCAG TGTCAGTACA GGATCAACTA CGCTGTAAAG 
GAAAGAGGAA AATATGTCTT AGCAGTGAAG TGGGGAGATG AGCACATTCC TGGATCGCCG 
TTTTACATTA GTGTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330212.1
CDS	996..7676
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGREVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCLVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEMCYSDEELDLDEDFPENVHPGGRGELVMNVFSPSNTPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLAVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGESCIVNIDCAKVGSEDLSVQAVSDSGVVVETEVKENDDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCLNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPLLDPQSLYGSSSPNQFQALPKYGRDKELSSAHDLNMTDIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVQFPNEESFNRQFQVLATDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVSYTPAMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGSHSIMVKYTNDDCFRRKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRGEPEANAKANGSIKVADIMDNRDKKCEMPCTPKMSGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTTEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSETTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITDGHMRKSEVKLGSAVDFTLDITEEDISQLSASIMSPTGNDVPCLLKTQADSHLGVSFIPREAGEHLVSIMKDGEHVSNSPISLSISQAEIGDASKVKAFGPGLHTGHTFCKSEFVVDTWDAGYGGLTVVIEGPSKVDVHTEELENDGTCNISYCPIKPGSYKISIKFSDEHIPGSPFTAVVKDQGLMRENITYNQKAAPTASIGSECSLAFKLPDVDPGTMSAHVYEPSGSFGEAVILPRTGRDIYVVSFLAKEMGVYNVSIQNKGQTISGCPLQYTVGPLGRGGSEKVQVWGQSLQTALATVPADFNIWSREAGAGALSVSVEGPGEIELHFDEQMDGSCIVTYTAQQPGDYEVSVMFNEDHVSQSPFLVSVIAATDTAVSDQTQLCNGHGEASLSGPQINTTSDSSAEPVLLSDASKVICHGPGLSKGFLGRKNTFYVDCSKAGRNLLFVGMHGPTVPCERVSIIHMGQCQYRINYAVKERGKYVLAVKWGDEHIPGSPFYISVV

Target ORF information:

RefSeq Version	XM_021474537.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474537.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTCGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCAACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGAGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTCGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCGTG TGATGACCAT AATGACGGCT CCTGTCTGGT GCGCTTCTGG 
CCGACCGAGG CCGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGTGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT GTGTTACTCT GATGAAGAGC TTGACCTCGA TGAAGACTTT 
CCAGAGAACG TCCATCCTGG TGGTCGAGGA GAACTGGTCA TGAATGTTTT CAGCCCGTCA 
AATACGCCGG CCGATGCCAC AAAGGTTAAT GCTCATGGAC CTGGACTGAC AGAAGGTGTT 
GTTGGAGAAC AAGCAGAGTT CATCATTGAC ACAAGCAGAG CAGGAAGTGG AAGACTTGCT 
GTAAGAGTAG ACGGACCCAG TGAGGTTACA CTACAATGTC TGGACGATCA AGATGGCACT 
TGCACTGTGC TTTACCTACC TACAGAGCAT GGAATGTACC AAATCAATGT GCTTTTTGAC 
AACTCTCACA TTCCTGGATC TCCGTTTGGG GCTAACATAC ATCATCCAGT TGACCCATCG 
AAAGTTTTGG TAACTAACTC TGCTTTGCAA CACGGAATAG TGGGTGAATC TTGCATTGTA 
AATATAGACT GTGCAAAGGT AGGATCTGAG GATCTATCAG TGCAGGCCGT TTCTGATTCA 
GGAGTTGTCG TTGAAACTGA AGTGAAAGAG AATGATGATG GCACATACAC TGTTGTTTAT 
GTGCCCCGAA CTGGAGGTGT ATATACACTG CTTCTCAAAT ATGGAGGAAA GGTGCTGCCT 
GTTCATAAAG TGATGGTGGA ACAGAAGGTC TACAAAAGCC ATGTAAAAGT GAGCAGAAGA 
GGAGGCGTTG TGGTTGGCGA AAGAGAAACT GATGCAAGCC AAGTTGTTGC AAGTGGTTTG 
GGTCTCCAGA AAGGCCTCAT CGGACATCCT AACATGTTTT GTTTAAACAC CAGATCTCCA 
GTTGATGATC TGGGTGTAAC AGTGGAGGGT CCGTCTGACT GTCAGGTGAC GTGTAAAGAT 
GGTGGTCAGG GCGTGTATGG TGTGGAATAT ACTCCAACAG TGCCTGGTGA ATATAAGATA 
TCCATAACCT CTGGAGAGAA CAGTATTCCA GGAAGCCCAT TTGTTGTGTC AGTGGATGAT 
GTTTCAAATA CTGCAGGCTC TGGAGATTGT CTACAGGCTG GGATTTGCAT ATCTTCAAAT 
GCTGTTACTT CTCAAGAAAT AGTCAAACAC AACGGATCCA GGATGCACAG AGCGAGTCCA 
GCTTCATTAG TGAAAGACAG AAAGCCCCTC TTAGATCCTC AAAGTCTTTA TGGAAGCAGC 
AGTCCCAACC AGTTTCAAGC ACTACCTAAG TATGGCCGTG ATAAAGAGCT TTCCTCAGCT 
CATGATCTGA ACATGACCGA TATTATTGCT CCTGGTATTA AAGGTGGAAT CTGTGGTAAA 
ACCCCTCAAG GTTTCACAAT TAGCTGCAGC AGCAGTGGAG AATCTCCAAA CGCAGTGGCT 
GTGGTAAGAC CTGATGGATC TAAGGAGTTC CTGGAGCTCA CAGACAACAG AGATGGCAGT 
TGTAGTGGTG TCTATTTACC CACCATGGAC GGTTGTCATT CATTGATAGT GCAATTCCCC 
AATGAAGAGA GTTTTAATAG ACAGTTCCAG GTACTGGCGA CAGATGAATT AGCGGAGGTT 
AATGTATCAG GTCCAGGTCT AACATCAGGA ATGTGTGCTC AGTGTCCTCA GACATTCATT 
ATCGACTGCA CTTCCTCAAG AAAAACTCTG CAGTTTGTTA CCATGATGAC ACCAAATGGC 
ATAAGTGAGA TAATGGAGGT CAGAGATAAT GGTGACTGCA CATACTCTGT GAGCTACACA 
CCTGCAATGG AAGGCCTTTA TTCTTTGATG GTCAAATATA CTGATGATGA TTCATTTTGC 
AGTCCCTTCA AGTTTCAAGT GCTCCCTGTG CCCGGTGTTG ATTATAAACA TCCTGAAACT 
CCAGTTTTTG AATCAGGCTT CAAGGCAGAT GTGTGTGTGA ACACTCCGCA GGCCTTCATT 
GTTGATGGCA GTAGCATTGG AGAGCCACCA GAGACTGTAG CTCTCCTTAC TCCTGATGGC 
AAAATCAAAT TATTAAGTTC AAAAGACAAT GGAGATGGGT CATATCTAGT TGAGTACTCC 
GCTTCTGCTG AAGGATCTCA CTCGATCATG GTCAAATATA CCAATGATGA TTGCTTCAGA 
AGAAAGGATA AAGACATTGG AAATAAAAAG GAAAATTCGA GTCAGGAGGG AAATGTCCAC 
TCTAAGCCTC CACATACATT CACTAACGGC TGCCGTGGGG AACCAGAAGC TAATGCTAAA 
GCTAATGGCT CAATAAAAGT CGCAGATATA ATGGACAACA GGGATAAAAA ATGTGAGATG 
CCTTGCACGC CAAAAATGTC AGGCTGTAAG TCTGTAATGG ACAAATACAA CAGCAAAGAA 
GCTTTCAGAA GCCTCCAGAC AGATCCGACT GTAAATACAG AGCAATCAGT CTGTGCTCAA 
AGTCCTCAGT TGTTCAGGAT CAGCTGTAGT AAATCCGGGA AGCCCCCTGA CTGTGTGCTG 
ATGATCAGAC CTGATGGAGA GACTGAACTG ACAGAGGTCA AAGAAAACCC TGACGGGACT 
TACTCTGTCA AATACTGTCC CACTAATGAA GGTCTTCATT CCTTGATGGT CAAATATGTT 
GATGATGATT CATTTTACAA TCCTTTGAGG TTTCATGTCC TTCCTCACGC TGATAGTCAT 
GACAAACATG TGATTAATAC AACTGAAGCT TGTAGCAGAC AATTAGAGGA ACGTGTCAGC 
GCTCAAATTC CTCAGACTTT CACAACTTGC TGCAACACTA ACGAGGCCTC AGGGACCGAG 
ATGTTGAAAG CTGATGATCA TGTTGAAGTG TTAAATAATG AGAACACAAC CAGTGAAACA 
ACACACACAC CTGAACCCTC AGCGGTCAGA AATGCCACAC TGGAGGATTT TCAAAGGCAC 
CAGCCACCAT CCTCTCACAC TTCAAGTGGG TACAGGGGTT TTCAAAACAA GGTTGAGGGC 
AGGAATGCAA TCCCTCCTGC TGCCATTAAT CAAGCAGGGT TTATATCCTG TGATATAGTC 
CTGCCAGGAG ACTTAAGTGA AGGTGAAATT ACAGGAGAGG TGATCACGCC GTCTGGAAAG 
AGAGCACAGC CTGACATGAT TGATAATAAA GATGGGACTG TCGTTATGAA GTTTGATCCT 
TCAGAGGAGG GTCTTCATCA AATCCTTATT AAATCAGCAG AAAATGATAT ACCAGAACTT 
CCACTGCAAT ATTATGCAAA TAGTTTGGCT AATCGCAGCC CTGTGGCCTA TGGGCGTGGC 
CTTGTGTGTG GAATTGCAAA TGAGACTGCA ACATTCACCA TCTGTCAGGA GGATTCAGCC 
TCGAGTGAAC TGGACATCAC AATTGAGGGT CCGTCTGAGG CTGATGTGCA CTGCTTGGAC 
AATGAAGATG GCACTTGTTC TGTGAGTTAC CTTCCAACTG AGCCTGGAGA TTATGAGATA 
CAAGTTCAAC ATAATGACGT GCCTATTCCA GGCAGTCCTT TCAAGGCCAA AATTACAGAT 
GGACATATGA GAAAGTCAGA GGTCAAACTG GGCAGTGCGG TCGATTTTAC CCTGGACATC 
ACAGAAGAAG ACATTAGCCA GCTCTCCGCT AGCATTATGT CGCCCACAGG CAATGATGTA 
CCCTGCTTAC TGAAAACACA AGCTGACAGC CATCTCGGCG TCTCCTTCAT CCCCAGAGAG 
GCTGGTGAGC ATCTGGTTAG CATCATGAAG GATGGGGAGC ATGTGAGCAA CAGTCCCATC 
TCTCTGTCCA TCAGTCAGGC AGAGATTGGA GATGCCAGTA AGGTGAAGGC TTTCGGTCCA 
GGCTTACACA CCGGGCACAC CTTCTGCAAG TCTGAATTTG TGGTGGACAC TTGGGATGCA 
GGATATGGTG GGTTAACAGT TGTAATTGAA GGTCCCAGTA AAGTAGACGT CCACACAGAA 
GAGCTGGAGA ATGATGGAAC TTGCAATATA TCATACTGCC CAATCAAGCC TGGCAGCTAT 
AAAATCTCCA TCAAATTCTC AGATGAGCAC ATTCCAGGGA GTCCGTTTAC AGCTGTGGTG 
AAAGACCAAG GGCTTATGAG AGAGAACATC ACATACAACC AGAAAGCTGC TCCTACTGCC 
AGCATTGGGA GTGAATGCAG TTTAGCCTTC AAACTCCCAG ATGTTGATCC TGGGACTATG 
TCTGCTCATG TATATGAACC ATCAGGAAGT TTTGGAGAGG CTGTTATCCT TCCTAGAACA 
GGCCGTGACA TCTATGTAGT GAGCTTTCTA GCTAAAGAGA TGGGAGTTTA TAATGTGAGC 
ATACAGAACA AAGGCCAGAC GATTTCCGGC TGCCCACTTC AATACACCGT CGGCCCGCTG 
GGACGAGGAG GATCTGAGAA GGTGCAGGTT TGGGGACAAA GCCTTCAGAC AGCTCTGGCA 
ACTGTTCCAG CTGACTTTAA TATTTGGTCG CGTGAGGCTG GAGCAGGGGC TCTGTCTGTC 
TCCGTAGAGG GTCCTGGAGA GATTGAGCTC CACTTTGATG AGCAGATGGA CGGGTCTTGC 
ATTGTGACCT ATACTGCACA ACAGCCTGGT GACTATGAGG TTTCAGTCAT GTTTAATGAG 
GATCATGTTT CCCAAAGCCC TTTTCTTGTG TCTGTCATTG CCGCCACAGA CACTGCTGTC 
TCTGATCAAA CTCAGCTGTG TAACGGTCAT GGAGAAGCCT CTCTGTCAGG ACCGCAGATC 
AACACAACAT CTGACAGCAG TGCTGAGCCA GTTTTGCTGT CTGACGCTAG TAAAGTCATC 
TGCCACGGCC CAGGCCTGAG TAAGGGCTTT CTGGGACGCA AAAACACTTT TTATGTGGAC 
TGCAGTAAAG CTGGACGAAA CCTTCTGTTC GTGGGCATGC ATGGCCCTAC AGTTCCCTGT 
GAGCGGGTGT CCATCATTCA CATGGGCCAG TGTCAGTACA GGATCAACTA CGCTGTAAAG 
GAAAGAGGAA AATATGTCTT AGCAGTGAAG TGGGGAGATG AGCACATTCC TGGATCGCCG 
TTTTACATTA GTGTTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_686387.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_691479.5 Danio rerio filamin B, beta (actin binding protein 278) (flnb), mRNA.

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CloneID	ODa27643
Clone ID Related Accession (Same CDS sequence)	XM_686387.7 , XM_021469933.1
Accession Version	XM_686387.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	filamin-A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_686387.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG 
GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG 
TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC 
ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG 
CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT 
AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT 
GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT 
AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC 
CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG 
AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC 
CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA 
GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG 
GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG 
ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT 
CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA 
AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT 
GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT 
GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG 
GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT 
GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC 
TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG 
TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC 
AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG 
CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC 
AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT 
GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_691479.5
CDS	344..7045
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGSEVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCQVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEICYSDEDFPENVHPGGRGELVMNVISPSNMPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLDVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGEPCIVNIDCAKVGSEDLSVQAISDSGVVVETEVKENEDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCVNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPVLDPQSLYGSSSPNQFQALPKYGRDKELSLAHDLNMADIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVKFPNEESFNRQFQVLPTDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVNYTPTMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGPHSIMAKYTNDDCFRSIHRFNAIPPHTVRKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRVEPEANAKANGSIKLADILDNRDKKCEMSCTPKMPGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTNEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSATTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITDGHMRKSEVKLGSAVDFTLDITEEDISQLSASIMSPTGNDVPCLLKTQADSHLGISFIPREAGEHLVSIMKDGEHVSNSPISLSISQAEIGDASKVKAFGPGLHTGHTFCKSEFVVDTWDAGYGGLTVVIEGPSKVDVHTKELENDGTCNISYCPIKPGSYKISIKFSDEHIPGSPFTAVVKDQGLMRENITYNQKAAPTASIGSECSLAFKLQDVDPGTMSAHVYEPSGSFGEAVILPSTGRDIYVVSFLAKEMGVYNVSIQNKGQTISGCPLQYTVGPLGRGGSEKVQVWGQSLQTALATVPADFNIWSREAGAGALSVSVEGPGEIELLFDEQMDGSCIVTYTAQQPGDYEVSVMFDEDHVSQSPFLVSVIAATDTAVSDQTQLCNGHGEASLSGPQINTTSDSSAEPVLLSDASKVICHGPGLSKGFLGRKNTFYVDCSKAGRNLLFVGMHGPTVPCERVSIIHMGQCQYRINYAVKERGKYVLAVKWGDEHIPGSPFYISVV

Target ORF information:

RefSeq Version	XM_686387.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_686387.7

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG 
GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG 
TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC 
ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG 
CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT 
AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT 
GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT 
AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC 
CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG 
AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC 
CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA 
GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG 
GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG 
ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT 
CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA 
AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT 
GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT 
GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG 
GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT 
GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC 
TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG 
TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC 
AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG 
CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC 
AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT 
GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flnb ( XM_021469933.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325608.1 Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X1, mRNA.

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CloneID	ODa27643
Clone ID Related Accession (Same CDS sequence)	XM_686387.7 , XM_021469933.1
Accession Version	XM_021469933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG 
GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG 
TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC 
ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG 
CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT 
AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT 
GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT 
AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC 
CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG 
AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC 
CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA 
GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG 
GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG 
ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT 
CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA 
AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT 
GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT 
GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG 
GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT 
GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC 
TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG 
TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC 
AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG 
CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC 
AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT 
GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325608.1
CDS	559..7260
Translation	MADFKCSVTSAAINLADDAPWKKIQKNTFTRWCNEHLRSVNMRISDLKTDLSDGLVLISLLEVLSHKRMFRKYHTRPTFRQLKLDNVSVALEFLDHEKVKLVSIDSKAIVDGNLKLILGLVWTLIQHYSISTPVWEDEANDSVSKLTPEMRLLGWIQNKVPELPITNFSQDWQDGKALGALVDGLAPGLCPDWESWDSVHRVSNTKEAMQQADDWLGVPQLIAPEEILDPAVDEQSVMTYLSLFPKARLKPGAPLKPKKVPKPKACRATGRGLQSKGIRVEQLAEFKVDTCRAGPGNLDVHIRDPSGSEVSVTQKELFKGVFGFQYTPSAEGDHTVDITWVGQHISKSPFKVNVGPEAGPQKIRVWGPGLEGGTVGSPAQFMVESVGADSGMLGFAIEGPSQAKIACDDHNDGSCQVRFWPTEAGEYAVHVMCDDEEIQDSPFMASIRPKSKGFHPDKVKVEGPGVSQTGCVINLQNEFIVDTRQAGEGELAVFAQRADGECVDVTISSEQSGVFVCSYTPSSLSKHTIAVTWGGVMVPGSPFIVTPRRIKITPCSPVWFKPDGSEICYSDEDFPENVHPGGRGELVMNVISPSNMPADATKVNAHGPGLTEGVVGEQAEFIIDTSRAGSGRLDVRVDGPSEVTLQCLDDQDGTCTVLYLPTEHGMYQINVLFDNSHIPGSPFGANIHHPVDPSKVLVTNSALQHGIVGEPCIVNIDCAKVGSEDLSVQAISDSGVVVETEVKENEDGTYTVVYVPRTGGVYTLLLKYGGKVLPVHKVMVEQKVYKSHVKVSRRGGVVVGERETDASQVVASGLGLQKGLIGHPNMFCVNTRSPVDDLGVTVEGPSDCQVTCKDGGQGVYGVEYTPTVPGEYKISITSGENSIPGSPFVVSVDDVSNTAGSGDCLQAGICISSNAVTSQEIVKHNGSRMHRASPASLVKDRKPVLDPQSLYGSSSPNQFQALPKYGRDKELSLAHDLNMADIIAPGIKGGICGKTPQGFTISCSSSGESPNAVAVVRPDGSKEFLELTDNRDGSCSGVYLPTMDGCHSLIVKFPNEESFNRQFQVLPTDELAEVNVSGPGLTSGMCAQCPQTFIIDCTSSRKTLQFVTMMTPNGISEIMEVRDNGDCTYSVNYTPTMEGLYSLMVKYTDDDSFCSPFKFQVLPVPGVDYKHPETPVFESGFKADVCVNTPQAFIVDGSSIGEPPETVALLTPDGKIKLLSSKDNGDGSYLVEYSASAEGPHSIMAKYTNDDCFRSIHRFNAIPPHTVRKDKDIGNKKENSSQEGNVHSKPPHTFTNGCRVEPEANAKANGSIKLADILDNRDKKCEMSCTPKMPGCKSVMDKYNSKEAFRSLQTDPTVNTEQSVCAQSPQLFRISCSKSGKPPDCVLMIRPDGETELTEVKENPDGTYSVKYCPTNEGLHSLMVKYVDDDSFYNPLRFHVLPHADSHDKHVINTNEACSRQLEERVSAQIPQTFTTCCNTNEASGTEMLKADDHVEVLNNENTTSATTHTPEPSAVRNATLEDFQRHQPPSSHTSSGYRGFQNKVEGRNAIPPAAINQAGFISCDIVLPGDLSEGEITGEVITPSGKRAQPDMIDNKDGTVVMKFDPSEEGLHQILIKSAENDIPELPLQYYANSLANRSPVAYGRGLVCGIANETATFTICQEDSASSELDITIEGPSEADVHCLDNEDGTCSVSYLPTEPGDYEIQVQHNDVPIPGSPFKAKITDGHMRKSEVKLGSAVDFTLDITEEDISQLSASIMSPTGNDVPCLLKTQADSHLGISFIPREAGEHLVSIMKDGEHVSNSPISLSISQAEIGDASKVKAFGPGLHTGHTFCKSEFVVDTWDAGYGGLTVVIEGPSKVDVHTKELENDGTCNISYCPIKPGSYKISIKFSDEHIPGSPFTAVVKDQGLMRENITYNQKAAPTASIGSECSLAFKLQDVDPGTMSAHVYEPSGSFGEAVILPSTGRDIYVVSFLAKEMGVYNVSIQNKGQTISGCPLQYTVGPLGRGGSEKVQVWGQSLQTALATVPADFNIWSREAGAGALSVSVEGPGEIELLFDEQMDGSCIVTYTAQQPGDYEVSVMFDEDHVSQSPFLVSVIAATDTAVSDQTQLCNGHGEASLSGPQINTTSDSSAEPVLLSDASKVICHGPGLSKGFLGRKNTFYVDCSKAGRNLLFVGMHGPTVPCERVSIIHMGQCQYRINYAVKERGKYVLAVKWGDEHIPGSPFYISVV

Target ORF information:

RefSeq Version	XM_021469933.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin B, beta (actin binding protein 278) (flnb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469933.1

ORF Insert Sequence:

ATGGCTGACT TTAAATGTAG CGTTACTTCA GCTGCAATCA ACCTGGCAGA TGATGCACCA 
TGGAAGAAGA TCCAGAAGAA CACCTTTACA CGCTGGTGCA ATGAACACCT GAGGTCTGTG 
AACATGCGGA TCAGCGATCT GAAGACCGAT CTGAGCGATG GCCTTGTTCT CATCTCTTTG 
CTGGAGGTCC TGAGCCACAA GAGGATGTTT AGAAAGTACC ACACGAGACC AACCTTCAGG 
CAACTCAAGC TGGACAATGT TTCGGTGGCT CTGGAGTTTT TGGATCATGA AAAGGTCAAG 
CTGGTGTCTA TTGACAGCAA AGCAATAGTG GATGGTAATT TGAAGCTGAT CCTGGGTCTG 
GTGTGGACCC TGATACAGCA TTACTCCATC TCCACCCCAG TGTGGGAGGA TGAGGCAAAC 
GATTCTGTCT CCAAACTGAC TCCAGAGATG AGGCTTCTGG GATGGATCCA AAACAAAGTT 
CCTGAACTGC CGATCACCAA CTTTAGCCAG GACTGGCAGG ATGGCAAGGC CCTCGGGGCA 
TTAGTGGATG GACTTGCACC TGGCTTGTGT CCTGACTGGG AGAGCTGGGA CTCTGTGCAT 
CGTGTGAGCA ACACTAAAGA AGCAATGCAG CAAGCTGATG ACTGGCTTGG GGTTCCTCAG 
CTCATAGCCC CAGAGGAGAT TCTCGATCCA GCTGTGGATG AGCAGTCGGT GATGACATAT 
CTCTCGCTTT TCCCGAAAGC CAGACTGAAA CCTGGAGCAC CGCTCAAGCC AAAGAAAGTG 
CCTAAACCAA AAGCCTGTCG TGCCACAGGA CGGGGATTGC AGTCCAAAGG CATAAGGGTA 
GAACAGCTGG CTGAATTTAA GGTGGACACA TGTCGAGCTG GGCCTGGAAA TCTGGATGTC 
CATATCAGAG ACCCCAGTGG AAGTGAAGTG TCGGTCACAC AAAAGGAGCT GTTCAAGGGT 
GTCTTTGGCT TCCAATATAC ACCCAGTGCT GAAGGAGATC ACACAGTGGA CATTACATGG 
GTTGGTCAGC ACATTTCCAA GAGCCCTTTT AAAGTCAATG TTGGTCCAGA AGCAGGACCA 
CAGAAGATCC GAGTGTGGGG ACCGGGACTA GAGGGAGGAA CTGTGGGCTC ACCGGCTCAG 
TTCATGGTGG AATCTGTTGG TGCTGATTCT GGAATGCTGG GTTTTGCCAT TGAAGGTCCA 
TCTCAGGCAA AGATCGCTTG TGATGACCAT AATGACGGAT CCTGTCAGGT GCGTTTCTGG 
CCGACCGAGG CAGGTGAGTA TGCAGTTCAC GTCATGTGTG ATGATGAAGA AATCCAGGAC 
AGTCCATTCA TGGCCTCCAT CAGACCCAAA AGCAAAGGCT TCCACCCTGA CAAGGTGAAA 
GTAGAAGGAC CAGGTGTTTC CCAAACTGGC TGCGTAATAA ACCTCCAAAA TGAATTCATC 
GTGGATACCA GACAGGCTGG TGAAGGAGAA CTTGCAGTTT TTGCTCAGAG AGCAGATGGA 
GAGTGTGTCG ATGTTACAAT CTCTTCTGAA CAATCTGGTG TGTTTGTCTG CTCCTACACG 
CCTTCATCTC TCTCCAAACA CACCATTGCT GTGACTTGGG GAGGCGTCAT GGTTCCTGGC 
AGTCCTTTCA TTGTGACACC TCGCAGGATT AAGATTACAC CTTGTAGCCC AGTCTGGTTT 
AAACCAGATG GATCAGAAAT TTGTTACTCT GATGAAGACT TTCCAGAGAA CGTCCATCCT 
GGTGGTCGAG GAGAACTGGT CATGAATGTT ATCAGCCCAT CAAATATGCC AGCCGATGCC 
ACAAAGGTAA ATGCTCATGG TCCTGGACTG ACAGAAGGTG TTGTTGGAGA ACAAGCAGAG 
TTCATTATTG ACACAAGCAG AGCAGGAAGT GGAAGACTTG ATGTAAGAGT AGACGGACCC 
AGTGAGGTTA CACTACAATG TCTGGACGAT CAAGATGGCA CTTGCACTGT GCTTTACCTC 
CCTACGGAGC ATGGAATGTA CCAAATCAAT GTGCTTTTTG ACAACTCTCA CATTCCTGGA 
TCTCCGTTTG GGGCTAACAT ACATCATCCA GTTGACCCAT CGAAAGTTTT GGTAACTAAC 
TCTGCTTTGC AACACGGAAT AGTGGGTGAA CCTTGCATTG TAAATATAGA CTGTGCAAAG 
GTAGGATCTG AGGATCTATC AGTGCAGGCC ATCTCTGATT CAGGAGTTGT CGTTGAAACC 
GAAGTGAAAG AGAACGAGGA TGGCACATAC ACTGTTGTTT ATGTGCCCCG AACTGGAGGT 
GTATATACAC TGCTTCTCAA ATATGGAGGA AAGGTGCTGC CTGTTCATAA AGTGATGGTG 
GAACAGAAGG TCTACAAAAG CCATGTAAAA GTGAGCAGAA GAGGAGGCGT TGTGGTTGGC 
GAAAGAGAAA CTGATGCAAG CCAAGTTGTT GCAAGTGGTT TGGGTCTCCA GAAAGGCCTC 
ATCGGACATC CTAACATGTT TTGTGTAAAC ACCAGATCTC CAGTTGATGA TCTTGGTGTA 
ACAGTGGAGG GTCCGTCTGA CTGTCAGGTG ACGTGTAAAG ATGGTGGTCA GGGCGTGTAT 
GGTGTGGAAT ATACTCCAAC AGTGCCTGGT GAATATAAGA TATCCATAAC CTCTGGAGAG 
AACAGTATTC CAGGAAGCCC ATTTGTTGTG TCAGTGGATG ATGTTTCAAA TACTGCAGGC 
TCTGGAGATT GTCTACAGGC TGGGATTTGC ATATCTTCAA ATGCTGTTAC TTCTCAAGAA 
ATAGTCAAAC ACAACGGATC CAGGATGCAC AGAGCGAGTC CAGCTTCATT AGTGAAAGAC 
AGAAAGCCCG TCTTAGATCC TCAAAGTCTT TATGGAAGCA GCAGTCCCAA CCAGTTTCAA 
GCACTACCTA AGTATGGCCG TGATAAAGAG CTTTCCTTAG CTCATGATCT GAACATGGCC 
GATATTATTG CTCCTGGTAT TAAAGGTGGA ATCTGTGGTA AAACCCCTCA AGGCTTCACA 
ATTAGCTGCA GCAGCAGTGG AGAATCTCCA AACGCAGTGG CTGTGGTGAG ACCTGATGGA 
TCTAAGGAGT TCCTGGAGCT CACAGACAAC AGAGATGGCA GTTGTAGTGG TGTCTATTTA 
CCCACCATGG ACGGTTGTCA TTCATTGATA GTGAAATTCC CCAATGAAGA GAGTTTTAAT 
AGACAGTTCC AGGTACTGCC GACAGATGAA TTAGCCGAGG TTAATGTATC AGGTCCAGGT 
CTAACATCAG GAATGTGTGC TCAGTGTCCT CAGACATTCA TTATCGACTG CACTTCCTCA 
AGAAAAACTC TGCAGTTTGT TACCATGATG ACACCAAATG GCATAAGTGA GATAATGGAG 
GTCAGAGATA ATGGTGACTG CACATACTCT GTGAACTACA CACCTACAAT GGAAGGCCTT 
TATTCTTTGA TGGTCAAATA TACTGATGAT GATTCATTTT GCAGTCCCTT CAAGTTTCAA 
GTGCTCCCTG TGCCCGGTGT TGATTATAAA CATCCTGAAA CTCCAGTTTT TGAATCAGGC 
TTCAAGGCAG ATGTGTGTGT GAACACTCCA CAGGCCTTCA TTGTTGATGG CAGTAGCATT 
GGAGAGCCAC CAGAGACTGT AGCTCTCCTT ACTCCTGATG GCAAAATCAA ATTATTAAGT 
TCAAAAGACA ATGGAGATGG GTCATATCTA GTTGAGTACT CCGCTTCTGC TGAAGGACCT 
CACTCGATCA TGGCCAAATA TACCAATGAT GATTGCTTCA GAAGTATTCA CAGGTTTAAT 
GCCATTCCTC CTCACACTGT CAGAAAGGAT AAAGACATTG GAAATAAAAA GGAAAATTCG 
AGTCAGGAGG GAAATGTCCA CTCTAAGCCT CCACATACAT TCACTAACGG CTGCCGTGTG 
GAACCAGAAG CTAATGCTAA AGCTAATGGC TCAATAAAAC TCGCAGATAT ATTGGACAAC 
AGGGATAAAA AATGTGAGAT GTCTTGCACA CCAAAAATGC CAGGCTGTAA GTCTGTAATG 
GACAAATACA ACAGCAAAGA AGCTTTCAGA AGCCTCCAGA CAGATCCGAC TGTAAATACA 
GAGCAATCAG TCTGTGCTCA AAGTCCTCAG TTGTTCAGGA TCAGCTGTAG TAAATCCGGG 
AAGCCCCCTG ACTGTGTGCT GATGATCAGA CCTGATGGAG AGACTGAACT GACAGAGGTC 
AAAGAAAACC CTGACGGGAC TTACTCTGTC AAATACTGTC CCACTAATGA AGGTCTTCAT 
TCCTTAATGG TCAAATATGT TGATGATGAT TCATTTTACA ATCCTTTGAG GTTTCATGTC 
CTTCCTCACG CTGATAGTCA TGACAAACAT GTGATTAATA CAAATGAAGC TTGTAGCAGA 
CAATTAGAGG AACGTGTCAG CGCTCAAATT CCTCAGACTT TCACAACTTG CTGCAACACT 
AACGAGGCCT CAGGGACAGA GATGTTGAAA GCTGATGATC ATGTTGAAGT GTTAAATAAT 
GAGAACACAA CCAGTGCAAC AACACACACA CCTGAACCCT CAGCGGTCAG AAATGCCACA 
CTGGAGGATT TTCAAAGGCA CCAGCCACCA TCCTCTCACA CTTCAAGTGG GTACAGGGGT 
TTTCAAAACA AGGTTGAGGG CAGGAATGCA ATCCCTCCTG CTGCCATTAA TCAAGCAGGG 
TTTATATCCT GTGATATAGT CCTGCCAGGA GACTTAAGTG AAGGTGAAAT TACAGGAGAG 
GTGATCACGC CGTCTGGAAA GAGAGCACAG CCTGACATGA TTGATAATAA AGATGGGACT 
GTCGTTATGA AGTTTGATCC TTCAGAGGAG GGTCTTCATC AAATCCTTAT TAAATCAGCA 
GAAAATGATA TACCAGAACT TCCACTGCAA TATTATGCAA ATAGTCTTGC TAATCGCAGC 
CCTGTGGCCT ATGGGCGTGG CCTTGTGTGT GGAATTGCAA ATGAGACTGC AACATTCACC 
ATCTGTCAGG AGGATTCAGC CTCGAGTGAA CTGGACATCA CAATTGAGGG TCCGTCTGAG 
GCTGATGTGC ACTGCTTGGA CAATGAAGAT GGCACTTGTT CTGTGAGTTA CCTTCCAACT 
GAGCCTGGAG ATTATGAGAT ACAAGTTCAA CATAATGACG TGCCTATTCC AGGCAGTCCT 
TTCAAGGCCA AAATTACAGA TGGACATATG AGAAAGTCAG AGGTCAAACT GGGCAGTGCG 
GTCGATTTTA CCCTGGACAT CACCGAAGAA GACATTAGCC AGCTCTCCGC TAGCATTATG 
TCGCCCACAG GCAATGATGT ACCCTGCTTA CTGAAAACAC AAGCTGACAG CCATCTCGGC 
ATCTCCTTCA TCCCCAGAGA GGCTGGTGAG CATCTGGTTA GCATCATGAA GGATGGGGAG 
CATGTGAGCA ACAGTCCCAT CTCTCTGTCC ATCAGTCAGG CAGAGATTGG AGATGCCAGT 
AAGGTGAAGG CCTTCGGTCC AGGCTTACAC ACCGGGCACA CCTTCTGCAA GTCTGAATTT 
GTGGTGGACA CTTGGGATGC AGGATATGGT GGCTTAACAG TTGTAATTGA AGGTCCCAGT 
AAAGTAGACG TCCACACAAA AGAGCTGGAG AATGATGGAA CTTGCAATAT ATCATACTGC 
CCAATCAAGC CTGGCAGCTA TAAAATCTCC ATCAAATTCT CAGATGAGCA CATTCCAGGG 
AGTCCGTTTA CAGCTGTGGT GAAAGACCAA GGGCTTATGA GAGAGAACAT CACATACAAC 
CAGAAAGCTG CTCCTACTGC CAGCATTGGG AGTGAATGCA GTTTAGCCTT CAAACTCCAA 
GATGTTGATC CTGGGACTAT GTCTGCTCAT GTATATGAAC CATCAGGAAG TTTTGGAGAG 
GCTGTTATCC TTCCTAGTAC AGGCCGTGAC ATCTATGTAG TGAGCTTTCT AGCTAAAGAG 
ATGGGAGTTT ATAATGTGAG CATACAGAAC AAAGGCCAGA CGATTTCCGG CTGCCCACTT 
CAATACACCG TCGGCCCGCT GGGACGAGGA GGATCTGAGA AGGTGCAGGT TTGGGGACAA 
AGCCTTCAGA CGGCTCTGGC AACTGTTCCA GCTGACTTTA ATATTTGGTC GCGTGAGGCT 
GGAGCAGGGG CTCTTTCTGT CTCCGTAGAG GGTCCTGGAG AGATTGAGCT CCTCTTTGAT 
GAGCAGATGG ACGGGTCTTG CATTGTGACC TATACTGCAC AACAGCCTGG TGACTATGAG 
GTTTCAGTCA TGTTTGATGA GGATCATGTT TCCCAAAGCC CTTTTCTTGT GTCTGTCATT 
GCCGCCACAG ACACTGCTGT CTCTGATCAA ACTCAGCTGT GTAACGGTCA TGGAGAAGCC 
TCTCTGTCAG GACCGCAGAT CAACACAACA TCTGACAGCA GTGCTGAGCC AGTTTTGCTG 
TCTGACGCTA GTAAAGTCAT CTGCCACGGC CCAGGCCTGA GTAAGGGCTT TCTGGGACGC 
AAAAACACTT TTTATGTGGA CTGCAGTAAA GCTGGACGAA ACCTTCTGTT CGTGGGCATG 
CATGGCCCTA CAGTTCCCTG TGAGCGGGTG TCCATCATTC ACATGGGCCA GTGTCAGTAC 
AGGATCAACT ACGCTGTAAA GGAAAGAGGA AAATATGTCT TAGCAGTGAA GTGGGGAGAT 
GAGCACATTC CTGGATCGCC GTTTTATATT AGTGTTGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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flnb cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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