man1b1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	man1b1a
Entrez Gene ID	562592
Full Name	mannosidase, alpha, class 1B, member 1a
Synonyms	si:ch211-181b18.1
General protein information	Preferred Names mannosidase, alpha, class 1B, member 1a Names endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469034.1	XP_021324709.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X2
XM_005161118.4	XP_005161175.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
XM_005161118.3	XP_005161175.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
XM_021473392.1	XP_021329067.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Man1b1	NP_001025154.1
Danio rerio (zebrafish)	si:ch211-181b18.1	XP_005161176.1
Caenorhabditis elegans (roundworm)	T03G11.4	NP_508877.1
Caenorhabditis elegans (roundworm)	ZC410.3	NP_001255362.1
Oryza sativa (rice)	Os05g0209100	NP_001054909.1
Xenopus tropicalis (tropical clawed frog)	man1b1	NP_001120156.1
Homo sapiens (human)	MAN1B1	NP_057303.2
Pan troglodytes (chimpanzee)	MAN1B1	XP_001135997.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003884	XP_003436647.1
Bos taurus (cattle)	MAN1B1	XP_002691756.3
Macaca mulatta (Rhesus monkey)	MAN1B1	XP_001091256.1
Canis lupus familiaris (dog)	MAN1B1	XP_537786.2
Gallus gallus (chicken)	MAN1B1	XP_415569.4
Drosophila melanogaster (fruit fly)	alpha-Man-Ib	NP_651667.1
Arabidopsis thaliana (thale cress)	MNS3	NP_564345.1
Eremothecium gossypii	AGOS_AER165W	NP_985023.1
Rattus norvegicus (Norway rat)	Man1b1	NP_001102666.1

Gene Ontology

The following man1b1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man1b1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146094	XM_021473392.1 Latest version!	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa25700	XM_005161118.4 Latest version!	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa25701	XM_021469034.1 Latest version!	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa25700	XM_005161118.3	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

man1b1a ( XM_021473392.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329067.1 Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146094
Clone ID Related Accession (Same CDS sequence)	XM_021473392.1
Accession Version	XM_021473392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395079.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGT CTTTGAGCGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTAGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGAGGTCTC 
AGGCCAATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAATTGGTGT TCAAGACGTC 
CAACCAGATG TTATACCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
AAGAATGCAG TGGAAGAAGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GTGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTAAAAC AGACTTCTAC TAGTAAACTA ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTAAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA AACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329067.1
CDS	364..2412
Translation	MKESLSGGLVEMYSPQKKDFVSLTVREQHNRGYTNGKHRRQSCWRKWKQLSRLQRSLILFLLAFLFICGILSYSSLTEQWRGISDRSLKEDWDEVDNRGLRPIPPGLIPKLSAGIGVQDVQPDVIPELQKPSIPLLPKPPAMRKPPSKRGPPALQKRMNVSDWQLKDGEEGLIKTDEDGAKEAKTVISWRGAMIEAEQGTELQSTIQGKDATVVDAEEAADLKTGTLKPVDRVEAVQEAFRHAWKGYKAFAWGHDELKPLSKTHGEWFGLGLTLIDALDTMWILGLKDEFAEARKWVETELSFSKNVDVNLFESTIRILGGLLSTYHLSGDTLFLDKAKDIGFRLMPAFKTPSKIPYSDVNIGKGTAHPPRWTTDSTVAEVTSIQLEFRELSRLTQDPQYKNAVEEVTKQVHRLEGKRDGLVPMFINTNSGKFTRRGAFTLGARADSYYEYLLKQWLQGGKKETELLEDYIQAIEGVKKNLLKQTSTSKLTFVGELSNGRLNPKMDHLVCFLPGTLALGAHNGLPADHMELAIQLIETCHQMYVQMETGLSPEIAHFNLQNHNGRDIDVKPADRHNLLRPETVESLFYMYRFTKDSKYRDWGWEILQSFNKHTRVPSGGYTSIGNVRDPLNPAPRDKMESFFLSETLKYLFLLFSDETELISLDKYVFNTEAHPLPIWPSAS

Target ORF information:

RefSeq Version	XM_021473392.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473392.1

ORF Insert Sequence:

ATGAAAGAGT CTTTGAGCGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTAGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGAGGTCTC 
AGGCCAATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAATTGGTGT TCAAGACGTC 
CAACCAGATG TTATACCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
AAGAATGCAG TGGAAGAAGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GTGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTAAAAC AGACTTCTAC TAGTAAACTA ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTAAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA AACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man1b1a ( XM_005161118.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161175.1 Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25700
Clone ID Related Accession (Same CDS sequence)	XM_005161118.4 , XM_005161118.3
Accession Version	XM_005161118.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161118.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGT CTTTGAGTGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTCGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGGGGTCTC 
AGGCCCATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAGTTGGTGT TCAAGACGTC 
CAACCAGATG TTATGCCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
CAGAATGCAG TGGAAGAGGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GAGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTGAAAC AGACTTCTAC TAGTAAACTG ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTTAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA CACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161175.1
CDS	337..2385
Translation	MKESLSGGLVEMYSPQKKDFVSLTVREQHNRGYTNGKHRRQSCWRKWKQLSRLQRSLILFLLAFLFICGILSYSSLTEQWRGISDRSLKEDWDEVDNRGLRPIPPGLIPKLSAGVGVQDVQPDVMPELQKPSIPLLPKPPAMRKPPSKRGPPALQKRMNVSDWQLKDGEEGLIKTDEDGAKEAKTVISWRGAMIEAEQGTELQSTIQGKDATVVDAEEAADLKTGTLKPVDRVEAVQEAFRHAWKGYKAFAWGHDELKPLSKTHGEWFGLGLTLIDALDTMWILGLKDEFAEARKWVETELSFSKNVDVNLFESTIRILGGLLSTYHLSGDTLFLDKAKDIGFRLMPAFKTPSKIPYSDVNIGKGTAHPPRWTTDSTVAEVTSIQLEFRELSRLTQDPQYQNAVEEVTKQVHRLEGKRDGLVPMFINTNSGKFTRRGAFTLGARADSYYEYLLKQWLQGGKKETELLEDYIQAIEGVKKNLLKQTSTSKLTFVGELSNGRLNPKMDHLVCFLPGTLALGAHNGLPADHMELAIQLIETCHQMYVQMETGLSPEIAHFNLQNHNGRDIDVKPADRHNLLRPETVESLFYMYRFTKDSKYRDWGWEILQSFNKHTRVPSGGYTSIGNVRDPLNPAPRDKMESFFLSETLKYLFLLFSDDTELISLDKYVFNTEAHPLPIWPSAS

Target ORF information:

RefSeq Version	XM_005161118.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161118.4

ORF Insert Sequence:

ATGAAAGAGT CTTTGAGTGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTCGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGGGGTCTC 
AGGCCCATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAGTTGGTGT TCAAGACGTC 
CAACCAGATG TTATGCCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
CAGAATGCAG TGGAAGAGGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GAGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTGAAAC AGACTTCTAC TAGTAAACTG ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTTAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA CACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man1b1a ( XM_005161118.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161175.1 Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), mRNA.

Price & Availability↑

CloneID	ODa25700
Clone ID Related Accession (Same CDS sequence)	XM_005161118.4 , XM_005161118.3
Accession Version	XM_005161118.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAGT CTTTGAGTGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTCGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGGGGTCTC 
AGGCCCATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAGTTGGTGT TCAAGACGTC 
CAACCAGATG TTATGCCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
CAGAATGCAG TGGAAGAGGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GAGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTGAAAC AGACTTCTAC TAGTAAACTG ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTTAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA CACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161175.1
CDS	323..2371
Translation	MKESLSGGLVEMYSPQKKDFVSLTVREQHNRGYTNGKHRRQSCWRKWKQLSRLQRSLILFLLAFLFICGILSYSSLTEQWRGISDRSLKEDWDEVDNRGLRPIPPGLIPKLSAGVGVQDVQPDVMPELQKPSIPLLPKPPAMRKPPSKRGPPALQKRMNVSDWQLKDGEEGLIKTDEDGAKEAKTVISWRGAMIEAEQGTELQSTIQGKDATVVDAEEAADLKTGTLKPVDRVEAVQEAFRHAWKGYKAFAWGHDELKPLSKTHGEWFGLGLTLIDALDTMWILGLKDEFAEARKWVETELSFSKNVDVNLFESTIRILGGLLSTYHLSGDTLFLDKAKDIGFRLMPAFKTPSKIPYSDVNIGKGTAHPPRWTTDSTVAEVTSIQLEFRELSRLTQDPQYQNAVEEVTKQVHRLEGKRDGLVPMFINTNSGKFTRRGAFTLGARADSYYEYLLKQWLQGGKKETELLEDYIQAIEGVKKNLLKQTSTSKLTFVGELSNGRLNPKMDHLVCFLPGTLALGAHNGLPADHMELAIQLIETCHQMYVQMETGLSPEIAHFNLQNHNGRDIDVKPADRHNLLRPETVESLFYMYRFTKDSKYRDWGWEILQSFNKHTRVPSGGYTSIGNVRDPLNPAPRDKMESFFLSETLKYLFLLFSDDTELISLDKYVFNTEAHPLPIWPSAS

Target ORF information:

RefSeq Version	XM_005161118.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161118.3

ORF Insert Sequence:

ATGAAAGAGT CTTTGAGTGG TGGTCTGGTA GAGATGTATT CACCACAGAA GAAGGATTTT 
GTGTCTTTGA CGGTTCGAGA ACAACACAAC CGTGGCTACA CCAACGGCAA ACACCGGAGA 
CAGTCCTGCT GGAGGAAATG GAAGCAGCTT TCTCGACTGC AGCGCAGCCT CATTCTCTTC 
CTGCTGGCCT TCCTCTTTAT ATGTGGGATT CTGTCCTATT CCAGTCTCAC GGAGCAGTGG 
AGAGGTATCT CTGATCGGTC ATTGAAGGAG GATTGGGATG AAGTGGATAA TCGGGGTCTC 
AGGCCCATTC CTCCTGGCCT CATCCCAAAG CTGTCTGCAG GAGTTGGTGT TCAAGACGTC 
CAACCAGATG TTATGCCAGA GCTTCAGAAA CCCAGCATTC CATTACTTCC TAAACCTCCA 
GCTATGAGAA AACCTCCTAG CAAGCGTGGT CCCCCAGCGC TACAGAAGCG AATGAACGTG 
TCCGACTGGC AGCTGAAAGA TGGAGAAGAA GGCTTGATCA AGACAGATGA AGACGGAGCA 
AAAGAAGCAA AAACAGTCAT TAGCTGGCGC GGGGCGATGA TTGAGGCAGA GCAGGGCACT 
GAGCTTCAGT CCACCATTCA GGGAAAGGAT GCAACTGTGG TTGATGCTGA GGAAGCTGCT 
GACTTAAAAA CAGGGACATT GAAGCCAGTG GATCGTGTGG AAGCGGTGCA GGAGGCCTTC 
AGGCATGCAT GGAAAGGTTA TAAGGCTTTT GCATGGGGTC ATGATGAGCT GAAGCCACTA 
TCTAAGACTC ATGGAGAGTG GTTTGGTCTG GGCCTCACGC TCATAGATGC ACTGGACACC 
ATGTGGATCC TTGGTCTGAA AGACGAGTTT GCAGAGGCCA GGAAGTGGGT GGAGACAGAA 
TTATCCTTCT CCAAGAATGT GGACGTCAAT CTTTTTGAGA GCACCATTCG CATTTTAGGA 
GGTCTCTTGA GCACATATCA CCTGAGTGGC GACACTCTCT TCCTGGATAA AGCTAAAGAC 
ATTGGTTTCA GATTAATGCC TGCCTTCAAA ACTCCATCTA AAATCCCCTA CTCTGACGTA 
AACATTGGTA AGGGAACGGC TCATCCTCCA CGCTGGACCA CAGACAGCAC AGTTGCAGAG 
GTGACCAGCA TTCAGCTGGA GTTCAGAGAG CTCAGCCGGC TCACACAGGA CCCGCAGTAT 
CAGAATGCAG TGGAAGAGGT GACCAAACAG GTCCACAGAT TGGAGGGGAA GCGTGATGGA 
CTGGTGCCAA TGTTCATCAA CACAAACAGT GGCAAATTCA CGCGGCGAGG GGCCTTTACC 
CTGGGGGCCC GAGCAGACAG TTACTACGAG TACCTGCTCA AACAGTGGCT TCAGGGCGGC 
AAGAAGGAGA CTGAGCTGCT GGAGGACTAT ATTCAGGCAA TAGAAGGTGT CAAGAAGAAT 
CTTTTGAAAC AGACTTCTAC TAGTAAACTG ACCTTTGTTG GAGAGCTTTC CAATGGCCGT 
CTTAATCCCA AAATGGATCA TTTGGTTTGT TTTCTTCCTG GAACACTGGC TCTCGGTGCC 
CACAACGGTC TGCCTGCAGA TCACATGGAG CTGGCCATTC AGCTGATCGA GACGTGCCAT 
CAGATGTATG TGCAGATGGA AACAGGTCTG AGTCCAGAGA TTGCTCACTT TAACCTGCAG 
AACCACAACG GACGAGACAT CGACGTGAAG CCTGCAGACA GACACAACTT GCTGAGACCC 
GAGACTGTTG AGAGTCTGTT CTACATGTAC AGATTCACCA AGGACAGCAA ATATAGGGAC 
TGGGGCTGGG AGATACTGCA GAGCTTCAAC AAGCACACCA GAGTTCCCAG TGGTGGCTAC 
ACTTCTATAG GTAACGTCCG GGACCCGTTA AACCCTGCAC CCAGAGACAA GATGGAGAGC 
TTCTTCCTGA GCGAGACGCT AAAGTATCTC TTCCTCCTGT TTTCTGATGA CACCGAGCTC 
ATCAGTCTGG ATAAATACGT GTTCAACACT GAAGCCCATC CGCTGCCCAT TTGGCCCTCA 
GCCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man1b1a ( XM_021469034.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324709.1 Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X2, mRNA.

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CloneID	ODa25701
Clone ID Related Accession (Same CDS sequence)	XM_021469034.1
Accession Version	XM_021469034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTCAC CACAGAAGAA GGATTTTGTG TCTTTGACGG TTCGAGAACA ACACAACCGT 
GGCTACACCA ACGGCAAACA CCGGAGACAG TCCTGCTGGA GGAAATGGAA GCAGCTTTCT 
CGACTGCAGC GCAGCCTCAT TCTCTTCCTG CTGGCCTTCC TCTTTATATG TGGGATTCTG 
TCCTATTCCA GTCTCACGGA GCAGTGGAGA GGTATCTCTG ATCGGTCATT GAAGGAGGAT 
TGGGATGAAG TGGATAATCG GGGTCTCAGG CCCATTCCTC CTGGCCTCAT CCCAAAGCTG 
TCTGCAGGAG TTGGTGTTCA AGACGTCCAA CCAGATGTTA TGCCAGAGCT TCAGAAACCC 
AGCATTCCAT TACTTCCTAA ACCTCCAGCT ATGAGAAAAC CTCCTAGCAA GCGTGGTCCC 
CCAGCGCTAC AGAAGCGAAT GAACGTGTCC GACTGGCAGC TGAAAGATGG AGAAGAAGGC 
TTGATCAAGA CAGATGAAGA CGGAGCAAAA GAAGCAAAAA CAGTCATTAG CTGGCGCGGG 
GCGATGATTG AGGCAGAGCA GGGCACTGAG CTTCAGTCCA CCATTCAGGG AAAGGATGCA 
ACTGTGGTTG ATGCTGAGGA AGCTGCTGAC TTAAAAACAG GGACATTGAA GCCAGTGGAT 
CGTGTGGAAG CGGTGCAGGA GGCCTTCAGG CATGCATGGA AAGGTTATAA GGCTTTTGCA 
TGGGGTCATG ATGAGCTGAA GCCACTATCT AAGACTCATG GAGAGTGGTT TGGTCTGGGC 
CTCACGCTCA TAGATGCACT GGACACCATG TGGATCCTTG GTCTGAAAGA CGAGTTTGCA 
GAGGCCAGGA AGTGGGTGGA GACAGAATTA TCCTTCTCCA AGAATGTGGA CGTCAATCTT 
TTTGAGAGCA CCATTCGCAT TTTAGGAGGT CTCTTGAGCA CATATCACCT GAGTGGCGAC 
ACTCTCTTCC TGGATAAAGC TAAAGACATT GGTTTCAGAT TAATGCCTGC CTTCAAAACT 
CCATCTAAAA TCCCCTACTC TGACGTAAAC ATTGGTAAGG GAACGGCTCA TCCTCCACGC 
TGGACCACAG ACAGCACAGT TGCAGAGGTG ACCAGCATTC AGCTGGAGTT CAGAGAGCTC 
AGCCGGCTCA CACAGGACCC GCAGTATCAG AATGCAGTGG AAGAGGTGAC CAAACAGGTC 
CACAGATTGG AGGGGAAGCG TGATGGACTG GTGCCAATGT TCATCAACAC AAACAGTGGC 
AAATTCACGC GGCGAGGGGC CTTTACCCTG GGGGCCCGAG CAGACAGTTA CTACGAGTAC 
CTGCTCAAAC AGTGGCTTCA GGGCGGCAAG AAGGAGACTG AGCTGCTGGA GGACTATATT 
CAGGCAATAG AAGGTGTCAA GAAGAATCTT TTGAAACAGA CTTCTACTAG TAAACTGACC 
TTTGTTGGAG AGCTTTCCAA TGGCCGTCTT AATCCCAAAA TGGATCATTT GGTTTGTTTT 
CTTCCTGGAA CACTGGCTCT CGGTGCCCAC AACGGTCTGC CTGCAGATCA CATGGAGCTG 
GCCATTCAGC TGATCGAGAC GTGCCATCAG ATGTATGTGC AGATGGAAAC AGGTCTGAGT 
CCAGAGATTG CTCACTTTAA CCTGCAGAAC CACAACGGAC GAGACATCGA CGTGAAGCCT 
GCAGACAGAC ACAACTTGCT GAGACCCGAG ACTGTTGAGA GTCTGTTCTA CATGTACAGA 
TTCACCAAGG ACAGCAAATA TAGGGACTGG GGCTGGGAGA TACTGCAGAG CTTCAACAAG 
CACACCAGAG TTCCCAGTGG TGGCTACACT TCTATAGGTA ACGTCCGGGA CCCGTTAAAC 
CCTGCACCCA GAGACAAGAT GGAGAGCTTC TTCCTGAGCG AGACGCTAAA GTATCTCTTC 
CTCCTGTTTT CTGATGACAC CGAGCTCATC AGTCTGGATA AATACGTGTT CAACACTGAA 
GCCCATCCGC TGCCCATTTG GCCCTCAGCC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324709.1
CDS	155..2170
Translation	MYSPQKKDFVSLTVREQHNRGYTNGKHRRQSCWRKWKQLSRLQRSLILFLLAFLFICGILSYSSLTEQWRGISDRSLKEDWDEVDNRGLRPIPPGLIPKLSAGVGVQDVQPDVMPELQKPSIPLLPKPPAMRKPPSKRGPPALQKRMNVSDWQLKDGEEGLIKTDEDGAKEAKTVISWRGAMIEAEQGTELQSTIQGKDATVVDAEEAADLKTGTLKPVDRVEAVQEAFRHAWKGYKAFAWGHDELKPLSKTHGEWFGLGLTLIDALDTMWILGLKDEFAEARKWVETELSFSKNVDVNLFESTIRILGGLLSTYHLSGDTLFLDKAKDIGFRLMPAFKTPSKIPYSDVNIGKGTAHPPRWTTDSTVAEVTSIQLEFRELSRLTQDPQYQNAVEEVTKQVHRLEGKRDGLVPMFINTNSGKFTRRGAFTLGARADSYYEYLLKQWLQGGKKETELLEDYIQAIEGVKKNLLKQTSTSKLTFVGELSNGRLNPKMDHLVCFLPGTLALGAHNGLPADHMELAIQLIETCHQMYVQMETGLSPEIAHFNLQNHNGRDIDVKPADRHNLLRPETVESLFYMYRFTKDSKYRDWGWEILQSFNKHTRVPSGGYTSIGNVRDPLNPAPRDKMESFFLSETLKYLFLLFSDDTELISLDKYVFNTEAHPLPIWPSAS

Target ORF information:

RefSeq Version	XM_021469034.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 1B, member 1a (man1b1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469034.1

ORF Insert Sequence:

ATGTATTCAC CACAGAAGAA GGATTTTGTG TCTTTGACGG TTCGAGAACA ACACAACCGT 
GGCTACACCA ACGGCAAACA CCGGAGACAG TCCTGCTGGA GGAAATGGAA GCAGCTTTCT 
CGACTGCAGC GCAGCCTCAT TCTCTTCCTG CTGGCCTTCC TCTTTATATG TGGGATTCTG 
TCCTATTCCA GTCTCACGGA GCAGTGGAGA GGTATCTCTG ATCGGTCATT GAAGGAGGAT 
TGGGATGAAG TGGATAATCG GGGTCTCAGG CCCATTCCTC CTGGCCTCAT CCCAAAGCTG 
TCTGCAGGAG TTGGTGTTCA AGACGTCCAA CCAGATGTTA TGCCAGAGCT TCAGAAACCC 
AGCATTCCAT TACTTCCTAA ACCTCCAGCT ATGAGAAAAC CTCCTAGCAA GCGTGGTCCC 
CCAGCGCTAC AGAAGCGAAT GAACGTGTCC GACTGGCAGC TGAAAGATGG AGAAGAAGGC 
TTGATCAAGA CAGATGAAGA CGGAGCAAAA GAAGCAAAAA CAGTCATTAG CTGGCGCGGG 
GCGATGATTG AGGCAGAGCA GGGCACTGAG CTTCAGTCCA CCATTCAGGG AAAGGATGCA 
ACTGTGGTTG ATGCTGAGGA AGCTGCTGAC TTAAAAACAG GGACATTGAA GCCAGTGGAT 
CGTGTGGAAG CGGTGCAGGA GGCCTTCAGG CATGCATGGA AAGGTTATAA GGCTTTTGCA 
TGGGGTCATG ATGAGCTGAA GCCACTATCT AAGACTCATG GAGAGTGGTT TGGTCTGGGC 
CTCACGCTCA TAGATGCACT GGACACCATG TGGATCCTTG GTCTGAAAGA CGAGTTTGCA 
GAGGCCAGGA AGTGGGTGGA GACAGAATTA TCCTTCTCCA AGAATGTGGA CGTCAATCTT 
TTTGAGAGCA CCATTCGCAT TTTAGGAGGT CTCTTGAGCA CATATCACCT GAGTGGCGAC 
ACTCTCTTCC TGGATAAAGC TAAAGACATT GGTTTCAGAT TAATGCCTGC CTTCAAAACT 
CCATCTAAAA TCCCCTACTC TGACGTAAAC ATTGGTAAGG GAACGGCTCA TCCTCCACGC 
TGGACCACAG ACAGCACAGT TGCAGAGGTG ACCAGCATTC AGCTGGAGTT CAGAGAGCTC 
AGCCGGCTCA CACAGGACCC GCAGTATCAG AATGCAGTGG AAGAGGTGAC CAAACAGGTC 
CACAGATTGG AGGGGAAGCG TGATGGACTG GTGCCAATGT TCATCAACAC AAACAGTGGC 
AAATTCACGC GGCGAGGGGC CTTTACCCTG GGGGCCCGAG CAGACAGTTA CTACGAGTAC 
CTGCTCAAAC AGTGGCTTCA GGGCGGCAAG AAGGAGACTG AGCTGCTGGA GGACTATATT 
CAGGCAATAG AAGGTGTCAA GAAGAATCTT TTGAAACAGA CTTCTACTAG TAAACTGACC 
TTTGTTGGAG AGCTTTCCAA TGGCCGTCTT AATCCCAAAA TGGATCATTT GGTTTGTTTT 
CTTCCTGGAA CACTGGCTCT CGGTGCCCAC AACGGTCTGC CTGCAGATCA CATGGAGCTG 
GCCATTCAGC TGATCGAGAC GTGCCATCAG ATGTATGTGC AGATGGAAAC AGGTCTGAGT 
CCAGAGATTG CTCACTTTAA CCTGCAGAAC CACAACGGAC GAGACATCGA CGTGAAGCCT 
GCAGACAGAC ACAACTTGCT GAGACCCGAG ACTGTTGAGA GTCTGTTCTA CATGTACAGA 
TTCACCAAGG ACAGCAAATA TAGGGACTGG GGCTGGGAGA TACTGCAGAG CTTCAACAAG 
CACACCAGAG TTCCCAGTGG TGGCTACACT TCTATAGGTA ACGTCCGGGA CCCGTTAAAC 
CCTGCACCCA GAGACAAGAT GGAGAGCTTC TTCCTGAGCG AGACGCTAAA GTATCTCTTC 
CTCCTGTTTT CTGATGACAC CGAGCTCATC AGTCTGGATA AATACGTGTT CAACACTGAA 
GCCCATCCGC TGCCCATTTG GCCCTCAGCC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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man1b1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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