HELZ2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELZ2
Entrez Gene ID	562461
Full Name	helicase with zinc finger 2, transcriptional coactivator
Synonyms	gb:eb955855
General protein information	Preferred Names helicase with zinc finger 2, transcriptional coactivator Names helicase with zinc finger domain 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198847.4	XP_003198895.2	helicase with zinc finger domain 2
XM_003198847.3	XP_003198895.2	helicase with zinc finger domain 2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PRIC285	XP_002798110.1
Canis lupus familiaris (dog)	HELZ2	XP_005635349.1
Mus musculus (house mouse)	Helz2	NP_898985.2
Gallus gallus (chicken)	HELZ2	XP_004947338.1
Danio rerio (zebrafish)	LOC562461	XP_003198895.2
Xenopus tropicalis (tropical clawed frog)	LOC101733680	XP_004920654.1
Rattus norvegicus (Norway rat)	Helz2	XP_230961.5
Homo sapiens (human)	HELZ2	NP_001032412.2
Pan troglodytes (chimpanzee)	HELZ2	XP_003317092.1

Gene Ontology

The following HELZ2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELZ2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12360	XM_003198847.4 Latest version!	Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa12360	XM_003198847.3	Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELZ2 ( XM_003198847.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003198895.2 Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.

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CloneID	ODa12360
Clone ID Related Accession (Same CDS sequence)	XM_003198847.4 , XM_003198847.3
Accession Version	XM_003198847.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	helicase with zinc finger domain 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198847.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG CAAAGAACCA CTATAAACTT CAAGCATTAC TACAGACGTA CACACTGTGT 
GTGAGATGCG GGAGGTGCTG TAATAAACAC AATAAAATTT CATACTCCCT CAACAATATC 
AAGCACAGCT GCTCAGAAAA CATTGTACTG ATCAGACGGA AACAAGACGG CTCATACTGG 
AGGCCTGTTG CACCCTTGCC AAACTTCCCC AGACCAAAGA AGTATGTTGC TTGCTGGTAC 
TACACTAAAG ATCGTGGATG CACAAGGCAT GGAAATAATT GTCAATTTGC GAGCAGCTCG 
GAGGAAGCAG ACTTGTGGAA TGTCATGAAA GATGAAAAAC TGACCATTCC TCAGATTGTG 
AATGCGATCA AACAAAACCA AAGGACACCG CAGATAACAT TTCAGGAGAA ACGAGGACCG 
CATGACTGCA CGTTATGTCA GGTCCACTTC CCAACGGACG AGGACTTCAT GAACCACTGT 
TTCACCGTAA AGCACAGAAG ACGGATCTTT GAAGATGGCA ATAAGACCTG GAAGCACCGC 
GAGCCGCCAC CAACACACAA GGACCTGAAA CTGTGTGAAA GATCCCTGAT ATGTGAGTAT 
GGAGATAACT GCACAAAGGC GCACAGTCAA GAGGAACTTC AAGAATGGCA AACAAGAGTC 
AGAGCTGTAC GAAATAAAGC CCGAGATGCA GCCAGTCAGG GTCTACTCTC CTACCAGGAC 
AATCTGCTGG ACGAATACAG GCACAGCAAA GACAAGAAGA TGATTATGAC TGACACCATT 
CCTGATGTGT TCATCACCTG TGACCGAGAT TTAGATGCTG TCTATGTAAG GAAAAGGGGA 
ATACAGTGTA CATGGAATTT TACCATCATA TCTAAGGAGC CTCTTGAAGT TATTGCTTTA 
CTCAGGCGGG AACAGGGGGC AAAATTCAGC CTCAGCAAAA AACCAGATGA GGAACGGTCG 
TATTCCACGA GCGCTTGGTT TGAAAAGAGG ACAGAGCAAA CTGGGCATGA AATCCCAGTG 
TCATTCAGAT CTGACCAGCC TGGCCTGTAT GAGCAGTGGG TAGTGTTTGA CTTCAATACG 
AGACCTGTAC TTCGACAAAA ACTAAAGGTC AGAGTTGGAG AGGATGATTG TCAGGCAGCT 
CCAGCACCGG AGGCACTATT AGAAAACCCA GAAGCTGCCT GCAATCTTGA GCCTTGGAAT 
GAGGAAAGCA TGACAATCAT TCCTTTTGTT GAAACAGAAA AAGCACAGAG AGACCTACTA 
AACAGATACC AACTCATCAT GATGAACCAG CAGATGAACG GTTTGATTAA CCGTGAGAAT 
TATAAATGGA GCATGCACAA TTTTTTATAC CAGGAGGAGA AGGTAGTCGG TGAACTGCTT 
TCCAGATTAA GTCTACGAGG GACTGTGCAG CTGAAAAATG AGTTGTCTGA TGACAGGTTT 
GGAATGAAAT GGGCTCACCC GGGTGAACTT TTTGCTGCTG TGCCTCTGTC TCAGCCACTC 
ACACCTGATG CTCCTCAAGG TTTCATTGTG AAAAGACATG CCATCAGATC TCTTGTTCGT 
GTTGTGAAGA ACAATGAATA TTGTGGGCCT ATTTATGAAG CTGAGTTTCT GAAAGATGCT 
GCCTCCGAGA CACAAGTACA TCTCCAGCTT TCCAAACGCT GCTGTGCTGC CCTTAACCTG 
CAGAATGGAG AGGAATATGA GATGGAGGTC CAGTTTCAAG TCAATCGTCT GTGGTTCTGC 
GAGATGCACA AGGCAATTGA TGATCTCCCG AGGATGGACA AAGTGTTGCC TGATCTGAAA 
AATGCTCACC TCAGTATTTC TTTCCAGTCA GATGAAGGAG AGTTCAATGA GAAGCAGCAA 
GCAGCAATGG ACTTTGTCTT GGGTGTGTCA GACAACAGAA GCAGCATCCC ACCCTTGTTG 
ATTTATGGAC CTTTTGGGAC TGGCAAAACT CGAACCCTTG CAAAGATGGC ACAGGCCCTT 
GTACAGCAGC CACACAACAA AATCTTAATT TGCACACACA CAAACAGTTC TGCTGATCTT 
TATATAAAAG CTCATTTCCA CGAATATGTA AGGAATGGCC AGCTTCACGC CAGACCTCTG 
CGAATAAAGG CAATGGAAAT AAAGCTCGCA TCCACCGATT TCATCACTCT ACAGTACTGC 
CATTTATCAA GAGAAGCCAA TTGCTTTGAG TTTCCTGATA AAGCAGTTTT AGATTCCACA 
AGAATCGTCA TAACAACAAC ATCTCTGGCT CGTTTCTTCA ACGACATGAA GCTTCCTGAA 
AACTACTTCA GCCATATTCT GATTGATGAA GCGTCTCAGA TGTTGGAGTG TGAGGCTCTT 
ATGGCACTTA GCTTAGCGGG TAACAATACA CGTGTGGTTT TAGCAGGAGA TCACATGCAG 
ATGGGACCCA AGCTGTTCTC TGTGAGGCCA GACAAATGTT CTGAGCACAC CCTGCTCAAT 
CGCCTCTTCT ATCACTACCA AGCCGAAAAC AGCGATGTGG CCAAACAAAG CCGGATCATT 
TTCAATGAAA ACTACCGCTC CACAAAAGAC ATTGTGGATT TTGTATCAAC ACATTTTTAT 
GTTGGAAGGA GTGATGTGAT CAAGGCTAGA GGAAATGTGC CGTCTCATCC CCAACAACAT 
GCTTTGCAGT TTCATCATGT AAGAGGAGAA TGCTGTCTGG ACCCAATGAG CATGTCCTGG 
TTCAATGCAG AACAAATTGT AAGAGTCGTT CACATTCTGC AAGAAATAAT GAAGGAATGG 
CCTCAAGAGT GGGGTGCTGC AGATCCAGAA TCAGTCTGTG TCCTATCTCA AGGCAGACAG 
GTGTTGGAAA TCAGGTATAG ACTGCGTTTG CTTGGACTGC CTCGTTTCAC TGTAGAGAAT 
GCTGAAAATG TGCAAGGAAA GCAGTTCAGA GTAATTGTGA TTTCCACTGT GCACACCAAG 
GACAGCCTAT TGGAGACGGA CAGGACCTGT CTTGAGTTTT TCAATGACAT GCGAGTCCTG 
AACACCATCA TGACAAGAGC CCAATCACAA ATATTTGTTG TTGGAGATGC CGCTGCACTT 
TCATGCCATC AGTTCGGTAT GTGCTGGAGA CTTTGGAGAG CATACATAGA GGAATGCATC 
AACAAGGAAA GTTGCCACGA CCTTACTCTG GATTCTTTAA AGCAAGATCT CCTGGAGATT 
TCTAAACTCT GCAGGAGCGA GGATGAAGAG AACAGCGATA GTGAATCAAC TACATCTGAA 
CTACCGGACA CTGAAGACCT AATACTTCAA GAGCTTCTTG ATGAGAGCAA AGAAATCAAT 
GTTGCTTTGA CAGAGGAAGG ATTATTTCCA GTTTTTCAAA ATCAGGAAGC ATTCAGTAGT 
GCAAATAACC AAGTTGCAGA AGTATATTAT GAAGAGCCTA TGTTTACTAG CTCTACTGTC 
CACAAACAGT GTGAGATAGT CAAAGAAGGT TATGACAGAG GATATGCAAA GCCACTTGAC 
GAGTCCACTC TGAGAATAGA CATCAAGGGT AGAGCGAACA TCGGAAGGGC ATTCCCTGGA 
GATAAAGTAA CGGTGGAGAT CCTGAGTGCA ACATCCCATC CTCCAATGGG AAGAGTGATC 
AACATCCTTG AAAGTGCAGA TACTGTCAAG GAATTTGTCT GCGCACTTGA AAGACATGAC 
AATCAAGTAA TGACGCCTAT AAACAAATGC ATCACCAAGA TCTTCACACC CTTTTGGAAA 
GACAAACCAA ACCACATTGC TGTAAGGAAT CCTGAGAGCC TCAAAGTAGA GAAGTTTATC 
AAAATCAACG AAGAAGCTCA CAGGAAATCT CTCTTTATTG TCAAAGTCCT TAGTTGGAGA 
AACGTTTTCA GATATCCTTT GGGGATTGTC GTAGGTGTGC TTCCTAAAAT AGCAACTTTA 
GATGATGGAT TGCATGTACT CAGCATTGAG TACCAGTTGA GAAGGACACT TTCCTCATCT 
CTGAGAAAGC AGATGCAATA CTTACAACGT CTTGATATTT TCGAAAAAGG ACGAAGAGAT 
TTTCGCATGC TCCCAACCTT CACAGTTGAT CCCGCACATT CGCAAGATCT CGATGATGCA 
ATAAGTTTGC AGGATTTTGG TGAGTACCTT GAAATAGGAG TTCACATTTC TGATGTTGCA 
AGCTTTGTGG CTAAAGATAG CGAACTGGAC AAATTTGCCA GACAGCTGTG TACTACATTC 
TACCCTCGTG AGAAGGAGCC AATATACATG TTTCCCAAAG AACTGAGCAC CAATTTGTTA 
AGTTTAATCC CTAACCAGGA CAGACGAGCA ATCTCATTGA TTATAGAAGT GGATGCACAG 
ACTAATCGCA TAAAGAGAAG ATTTATCTGC AAGTCTGTGA TTCACTCCAA CAGGAAATTT 
TCCTACGATG AAGTTGAGGA CATTCTTGAA AATGGTGAGC CGCTTTCCGA CCTGCTTGAA 
ATCTGTCTTG TAAAAGTGTC GAACTTTGCT GAGGTTCACC GAAGATACAG AAAGCAGGAT 
GACTGGTGCT ACAAGATGCC AGATGAGGAT GTGACCCTTG GAAGAAGGAG ATCTCACCAA 
ATGGTGGAAG AGCTGATGGT CATGTTTAAC CACATGGTTG CCGAACGTCT TTTATCTGAT 
GGTGCAACAA GAAGCAAAAC TCCATTGAGA TGCCAGGACA GGCCTCAAAC AGACAAACTC 
ATTGAGATTT TTGACAAAAA CGCAGCCTTG ATTCCGCTGT CTGTTCACTT CTCAAGTCAA 
GCTCATGAAC TGAATAAGCA AGGTTTGATC CAGCACAACC CTCAGACTGA CTCAGACACG 
TTCCCTGTGT TAAAATCAAT CCTCAGATGC CTGAAGTCAG CAGCAGAGCA GGAAAACATC 
TACAAAATAA TTGACTTTGT TGCAACTGAT GACATTCATC CTCAGCTTCT GCCTTTTGCT 
GTTGCATTTA GGCGGTTGTT TCACAAGGCG TGCATTCAGC GCTCAAACTC AACGCATGTC 
TCAAGAATAG GGCACTATGA TTTAGATCTT GACAGCTATA CATGGGCCTC ATCTCCAGTT 
CGCCGATACT TAGATGTAAT TGTGCAGCGC CTTCTTCATT CTGTGATTGA CAAGACAGAT 
GTTAAGTACA CAACTCTAGA CATTAACCTG TCTTGTTTGG AATTCTCCAG GAAAAATGAT 
CAACAGTCGG CCTATGAGAG GAAGGCAAAT GCTCTGCATT TCGCATTAAA ACTGTCAAGC 
CAAAGTGAAA GGAAGGTGGC TTATATTGTG GAGCTCAATC CATTAGGGAC AAACTTCAAA 
GTGTCCTTTC CACTTAACAG ATCCTCTATA TCAGAAAACT TAGACATTAT GAGCAGGGAT 
CTTCTGCTGG CTGTTCAGCC AGAGTTTGAT GTAACAAACA ACAGCATGAT CCTGAAATGG 
GAGCGAAGGA TATATTCATT CTCCAATTCT CACATCCATG CTGAAGTGAA GCAACAAAAC 
CCAAACTCAG TTATGGCCTA TGTCCCTGCA AAGACCTGGA AATGCATTGC AGATGCTATC 
AGAGAACAAA ACTGGAATGC CATTTTTCCT CTTATTGAGG AACTTCAACG CAACTCTGGC 
ATCAATGCAA GACAATTTCA CGAAACACTG AGTGCTTCAA AATGCACGAA AAATTATGAC 
CCCTTGCATG CTGAGCATTA TGCTGAACTG TATCTGAGAA TTAAGCAAGG TGAGGCGGTC 
GAAGTGCAGC TTGGTGCATC CACAGCTCGT GGTTTAATGA CTCCATCGAT TCAGCTGTTT 
GTTGTGCATC CAAAGTTTGA GATTTGCGTG GAGCATGTAA GAGATCCAGT CAAGTGTTTT 
TCTGAATACG CACTAAGCTC ATCAAGGAGT TCATACAACT TGTACAAGGA TTATCAGGCA 
ATATGGAGAC CTATGTGCGA GATGGAGTCA GCCTCCAGCG CTGTGGCTGA AAATGAGAGC 
ATTCTTATTG TAGATGTGCC CTTGAAATGG GAAAACTCCA ATCCGAAGAG GCTACAGGGG 
TTTTTTCTGC TCCCGCTTGA CAAGAAATCT CAGTGGTCAA TAGACTGTGA TCTCAGAAAC 
AGCTTCCTAT GCATTCGAAC GAGGATTCAG AAACAGAGTA GCCCATTTCT GGAGAGCGCT 
CAGAACACAG CGCTCATGGA TATTCCTTCT TTCACTTGGG TAGTTCATGG TGTGATTACA 
GAAGCTGAAG AAACAGAGTC CAAAGAATCA ACGAATCAAG AACCAAAGAG TTTGCGAATT 
GATTTTCTGA TTAATCACAT GCCCATGACA AACATTCCAG AACAAATATT CCACGAGAGG 
ACGAGATTTA CGCTGGAGCT GATTCCAAAG CTTCTGCCAG ACGTGCGCAA AGAAAATGCA 
ATTGACAGTC TAACCAAAGC CAACCAGCTT GTAAAAAACA TTGCCATGGG CAGGAGAATC 
ACTTCGGACA GAGGATGTAA AATACCAAAT CAGAATACAG GCAGGTTTGA GATCAACAGT 
TATCATCCTT CTGGGTTATC CCATCTAAAC AACAGCCAAA GTAAAGCAAT AAGAGAAGCA 
CTGACTAACC CATTCACACT TATCCAAGGA CCACCGGGGA CTGGAAAAAC TGTTGTTGGT 
GTCCATATTT TGTACTGGCT GCACAAGAAT ATCCAACAGC TCCAAGCTTC TGGTTCAAAG 
AAGAGAGCTA TTCTGTACTG TGGCCCGTCA AATAAATCTG TTGATGTTGT TGCAGGTCAT 
CTCCTACATC TCCGAGACCA TCTTAAACCT CTCCGGGTCT ACAGTGATCA GATGGAGATG 
TTGGAGTTTC CGTACCCAGG CTGCAATCTG AAGCTGTCGC GTAACTCTAA AAGAGGAGAA 
AAACCCAACA CTGAGCTCAG TTCCATTGCC TTACACCATC TGATTCGAAA GGAGGGCAAC 
CCATTCTCCA CACAAATACG TGCTTTTGAT ATGAAAGTCC AAAGAGGAGA TCAACTCACT 
GATGAAGAAA GAAAACTCTT CAGAGAAATC CTCAAAAAGG CTCGAAAGCA TGAATTATTG 
CACCATGACG TAATCTTATG CACGTGCACC GCAGCTTCAC ATCCAGCACT GGCGGAAACC 
CTTGACTTTA AGCAGATCAT CATTGATGAA TGTGCCATGG CCACTGAACC TGAAGCCTTT 
ATTCCTCTCG TGGCTCATAA ACCTGAGCAG ATTGTCCTTC TTGGCGATCA CAAACAGCTT 
CAACCAGTGG TCCACTGCGA GGTGGCGGAG CGATTAGGAA TGAGCAGATC TCTTTTTGAA 
CGTTACATGG AGAACGCACT CATGCTTGAC ACCCAGTACA GAATGCAAGA AGACATTTGT 
GCATTTCCAT CTAATGAGTT CTATGGGGGG AGATTACAAA CTGGAATACC AGCCAAATCA 
AAACTATTCC TTACTCAAAC AAAGCAAACC TGCATCGTTT TTGGTCATGT TGAAGGAAAG 
GAGAAAAGTC TGGTGGTTCG AACTGAACAA GGCAATGAAA ACTCAAAGGC AAATATGGAA 
GAGGCAGAGG AAGTGGTGCG GGTCGCCATT CTTTTGACTG AGGCTGGGAT CAAGACAAAG 
GACATTGCCA TTCTCACACC ATATAATGCT CAAGTGGCCA AAATTAGTGA GTCTCTGCAT 
AATAACCATA TACGAGACAT CACCGTCAAC ACCATCATGA GGAGTCAAGG TAGTGAATGG 
AGGTACGTCA TCATGTCAAC AGTGCGCTCT TGTCCCAAGT CTGAAATAGA GACACAACCA 
CCCAAATCCT GGATTATGAA GCGGCTTGGA TTCATCACCG ACCCTCATCA AGTAAACGTT 
GGCATTACCA GAGCACAAGA AGGGCTGTGC ATCATCGGAA ATGAGAACTT GCTGCGCTGT 
AGTTCCTTAT GGAGGAAACT TCTGAATCAT TATCAGGAGA AAGGATGTGT GGTGAATCCT 
GCCCGGCTCA TCCAAGTCCG ACAACCCCCA AGAAGAACTG CTAGAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198895.2
CDS	414..8384
Translation	MALAKNHYKLQALLQTYTLCVRCGRCCNKHNKISYSLNNIKHSCSENIVLIRRKQDGSYWRPVAPLPNFPRPKKYVACWYYTKDRGCTRHGNNCQFASSSEEADLWNVMKDEKLTIPQIVNAIKQNQRTPQITFQEKRGPHDCTLCQVHFPTDEDFMNHCFTVKHRRRIFEDGNKTWKHREPPPTHKDLKLCERSLICEYGDNCTKAHSQEELQEWQTRVRAVRNKARDAASQGLLSYQDNLLDEYRHSKDKKMIMTDTIPDVFITCDRDLDAVYVRKRGIQCTWNFTIISKEPLEVIALLRREQGAKFSLSKKPDEERSYSTSAWFEKRTEQTGHEIPVSFRSDQPGLYEQWVVFDFNTRPVLRQKLKVRVGEDDCQAAPAPEALLENPEAACNLEPWNEESMTIIPFVETEKAQRDLLNRYQLIMMNQQMNGLINRENYKWSMHNFLYQEEKVVGELLSRLSLRGTVQLKNELSDDRFGMKWAHPGELFAAVPLSQPLTPDAPQGFIVKRHAIRSLVRVVKNNEYCGPIYEAEFLKDAASETQVHLQLSKRCCAALNLQNGEEYEMEVQFQVNRLWFCEMHKAIDDLPRMDKVLPDLKNAHLSISFQSDEGEFNEKQQAAMDFVLGVSDNRSSIPPLLIYGPFGTGKTRTLAKMAQALVQQPHNKILICTHTNSSADLYIKAHFHEYVRNGQLHARPLRIKAMEIKLASTDFITLQYCHLSREANCFEFPDKAVLDSTRIVITTTSLARFFNDMKLPENYFSHILIDEASQMLECEALMALSLAGNNTRVVLAGDHMQMGPKLFSVRPDKCSEHTLLNRLFYHYQAENSDVAKQSRIIFNENYRSTKDIVDFVSTHFYVGRSDVIKARGNVPSHPQQHALQFHHVRGECCLDPMSMSWFNAEQIVRVVHILQEIMKEWPQEWGAADPESVCVLSQGRQVLEIRYRLRLLGLPRFTVENAENVQGKQFRVIVISTVHTKDSLLETDRTCLEFFNDMRVLNTIMTRAQSQIFVVGDAAALSCHQFGMCWRLWRAYIEECINKESCHDLTLDSLKQDLLEISKLCRSEDEENSDSESTTSELPDTEDLILQELLDESKEINVALTEEGLFPVFQNQEAFSSANNQVAEVYYEEPMFTSSTVHKQCEIVKEGYDRGYAKPLDESTLRIDIKGRANIGRAFPGDKVTVEILSATSHPPMGRVINILESADTVKEFVCALERHDNQVMTPINKCITKIFTPFWKDKPNHIAVRNPESLKVEKFIKINEEAHRKSLFIVKVLSWRNVFRYPLGIVVGVLPKIATLDDGLHVLSIEYQLRRTLSSSLRKQMQYLQRLDIFEKGRRDFRMLPTFTVDPAHSQDLDDAISLQDFGEYLEIGVHISDVASFVAKDSELDKFARQLCTTFYPREKEPIYMFPKELSTNLLSLIPNQDRRAISLIIEVDAQTNRIKRRFICKSVIHSNRKFSYDEVEDILENGEPLSDLLEICLVKVSNFAEVHRRYRKQDDWCYKMPDEDVTLGRRRSHQMVEELMVMFNHMVAERLLSDGATRSKTPLRCQDRPQTDKLIEIFDKNAALIPLSVHFSSQAHELNKQGLIQHNPQTDSDTFPVLKSILRCLKSAAEQENIYKIIDFVATDDIHPQLLPFAVAFRRLFHKACIQRSNSTHVSRIGHYDLDLDSYTWASSPVRRYLDVIVQRLLHSVIDKTDVKYTTLDINLSCLEFSRKNDQQSAYERKANALHFALKLSSQSERKVAYIVELNPLGTNFKVSFPLNRSSISENLDIMSRDLLLAVQPEFDVTNNSMILKWERRIYSFSNSHIHAEVKQQNPNSVMAYVPAKTWKCIADAIREQNWNAIFPLIEELQRNSGINARQFHETLSASKCTKNYDPLHAEHYAELYLRIKQGEAVEVQLGASTARGLMTPSIQLFVVHPKFEICVEHVRDPVKCFSEYALSSSRSSYNLYKDYQAIWRPMCEMESASSAVAENESILIVDVPLKWENSNPKRLQGFFLLPLDKKSQWSIDCDLRNSFLCIRTRIQKQSSPFLESAQNTALMDIPSFTWVVHGVITEAEETESKESTNQEPKSLRIDFLINHMPMTNIPEQIFHERTRFTLELIPKLLPDVRKENAIDSLTKANQLVKNIAMGRRITSDRGCKIPNQNTGRFEINSYHPSGLSHLNNSQSKAIREALTNPFTLIQGPPGTGKTVVGVHILYWLHKNIQQLQASGSKKRAILYCGPSNKSVDVVAGHLLHLRDHLKPLRVYSDQMEMLEFPYPGCNLKLSRNSKRGEKPNTELSSIALHHLIRKEGNPFSTQIRAFDMKVQRGDQLTDEERKLFREILKKARKHELLHHDVILCTCTAASHPALAETLDFKQIIIDECAMATEPEAFIPLVAHKPEQIVLLGDHKQLQPVVHCEVAERLGMSRSLFERYMENALMLDTQYRMQEDICAFPSNEFYGGRLQTGIPAKSKLFLTQTKQTCIVFGHVEGKEKSLVVRTEQGNENSKANMEEAEEVVRVAILLTEAGIKTKDIAILTPYNAQVAKISESLHNNHIRDITVNTIMRSQGSEWRYVIMSTVRSCPKSEIETQPPKSWIMKRLGFITDPHQVNVGITRAQEGLCIIGNENLLRCSSLWRKLLNHYQEKGCVVNPARLIQVRQPPRRTARR

Target ORF information:

RefSeq Version	XM_003198847.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198847.4

ORF Insert Sequence:

ATGGCTTTAG CAAAGAACCA CTATAAACTT CAAGCATTAC TACAGACGTA CACACTGTGT 
GTGAGATGCG GGAGGTGCTG TAATAAACAC AATAAAATTT CATACTCCCT CAACAATATC 
AAGCACAGCT GCTCAGAAAA CATTGTACTG ATCAGACGGA AACAAGACGG CTCATACTGG 
AGGCCTGTTG CACCCTTGCC AAACTTCCCC AGACCAAAGA AGTATGTTGC TTGCTGGTAC 
TACACTAAAG ATCGTGGATG CACAAGGCAT GGAAATAATT GTCAATTTGC GAGCAGCTCG 
GAGGAAGCAG ACTTGTGGAA TGTCATGAAA GATGAAAAAC TGACCATTCC TCAGATTGTG 
AATGCGATCA AACAAAACCA AAGGACACCG CAGATAACAT TTCAGGAGAA ACGAGGACCG 
CATGACTGCA CGTTATGTCA GGTCCACTTC CCAACGGACG AGGACTTCAT GAACCACTGT 
TTCACCGTAA AGCACAGAAG ACGGATCTTT GAAGATGGCA ATAAGACCTG GAAGCACCGC 
GAGCCGCCAC CAACACACAA GGACCTGAAA CTGTGTGAAA GATCCCTGAT ATGTGAGTAT 
GGAGATAACT GCACAAAGGC GCACAGTCAA GAGGAACTTC AAGAATGGCA AACAAGAGTC 
AGAGCTGTAC GAAATAAAGC CCGAGATGCA GCCAGTCAGG GTCTACTCTC CTACCAGGAC 
AATCTGCTGG ACGAATACAG GCACAGCAAA GACAAGAAGA TGATTATGAC TGACACCATT 
CCTGATGTGT TCATCACCTG TGACCGAGAT TTAGATGCTG TCTATGTAAG GAAAAGGGGA 
ATACAGTGTA CATGGAATTT TACCATCATA TCTAAGGAGC CTCTTGAAGT TATTGCTTTA 
CTCAGGCGGG AACAGGGGGC AAAATTCAGC CTCAGCAAAA AACCAGATGA GGAACGGTCG 
TATTCCACGA GCGCTTGGTT TGAAAAGAGG ACAGAGCAAA CTGGGCATGA AATCCCAGTG 
TCATTCAGAT CTGACCAGCC TGGCCTGTAT GAGCAGTGGG TAGTGTTTGA CTTCAATACG 
AGACCTGTAC TTCGACAAAA ACTAAAGGTC AGAGTTGGAG AGGATGATTG TCAGGCAGCT 
CCAGCACCGG AGGCACTATT AGAAAACCCA GAAGCTGCCT GCAATCTTGA GCCTTGGAAT 
GAGGAAAGCA TGACAATCAT TCCTTTTGTT GAAACAGAAA AAGCACAGAG AGACCTACTA 
AACAGATACC AACTCATCAT GATGAACCAG CAGATGAACG GTTTGATTAA CCGTGAGAAT 
TATAAATGGA GCATGCACAA TTTTTTATAC CAGGAGGAGA AGGTAGTCGG TGAACTGCTT 
TCCAGATTAA GTCTACGAGG GACTGTGCAG CTGAAAAATG AGTTGTCTGA TGACAGGTTT 
GGAATGAAAT GGGCTCACCC GGGTGAACTT TTTGCTGCTG TGCCTCTGTC TCAGCCACTC 
ACACCTGATG CTCCTCAAGG TTTCATTGTG AAAAGACATG CCATCAGATC TCTTGTTCGT 
GTTGTGAAGA ACAATGAATA TTGTGGGCCT ATTTATGAAG CTGAGTTTCT GAAAGATGCT 
GCCTCCGAGA CACAAGTACA TCTCCAGCTT TCCAAACGCT GCTGTGCTGC CCTTAACCTG 
CAGAATGGAG AGGAATATGA GATGGAGGTC CAGTTTCAAG TCAATCGTCT GTGGTTCTGC 
GAGATGCACA AGGCAATTGA TGATCTCCCG AGGATGGACA AAGTGTTGCC TGATCTGAAA 
AATGCTCACC TCAGTATTTC TTTCCAGTCA GATGAAGGAG AGTTCAATGA GAAGCAGCAA 
GCAGCAATGG ACTTTGTCTT GGGTGTGTCA GACAACAGAA GCAGCATCCC ACCCTTGTTG 
ATTTATGGAC CTTTTGGGAC TGGCAAAACT CGAACCCTTG CAAAGATGGC ACAGGCCCTT 
GTACAGCAGC CACACAACAA AATCTTAATT TGCACACACA CAAACAGTTC TGCTGATCTT 
TATATAAAAG CTCATTTCCA CGAATATGTA AGGAATGGCC AGCTTCACGC CAGACCTCTG 
CGAATAAAGG CAATGGAAAT AAAGCTCGCA TCCACCGATT TCATCACTCT ACAGTACTGC 
CATTTATCAA GAGAAGCCAA TTGCTTTGAG TTTCCTGATA AAGCAGTTTT AGATTCCACA 
AGAATCGTCA TAACAACAAC ATCTCTGGCT CGTTTCTTCA ACGACATGAA GCTTCCTGAA 
AACTACTTCA GCCATATTCT GATTGATGAA GCGTCTCAGA TGTTGGAGTG TGAGGCTCTT 
ATGGCACTTA GCTTAGCGGG TAACAATACA CGTGTGGTTT TAGCAGGAGA TCACATGCAG 
ATGGGACCCA AGCTGTTCTC TGTGAGGCCA GACAAATGTT CTGAGCACAC CCTGCTCAAT 
CGCCTCTTCT ATCACTACCA AGCCGAAAAC AGCGATGTGG CCAAACAAAG CCGGATCATT 
TTCAATGAAA ACTACCGCTC CACAAAAGAC ATTGTGGATT TTGTATCAAC ACATTTTTAT 
GTTGGAAGGA GTGATGTGAT CAAGGCTAGA GGAAATGTGC CGTCTCATCC CCAACAACAT 
GCTTTGCAGT TTCATCATGT AAGAGGAGAA TGCTGTCTGG ACCCAATGAG CATGTCCTGG 
TTCAATGCAG AACAAATTGT AAGAGTCGTT CACATTCTGC AAGAAATAAT GAAGGAATGG 
CCTCAAGAGT GGGGTGCTGC AGATCCAGAA TCAGTCTGTG TCCTATCTCA AGGCAGACAG 
GTGTTGGAAA TCAGGTATAG ACTGCGTTTG CTTGGACTGC CTCGTTTCAC TGTAGAGAAT 
GCTGAAAATG TGCAAGGAAA GCAGTTCAGA GTAATTGTGA TTTCCACTGT GCACACCAAG 
GACAGCCTAT TGGAGACGGA CAGGACCTGT CTTGAGTTTT TCAATGACAT GCGAGTCCTG 
AACACCATCA TGACAAGAGC CCAATCACAA ATATTTGTTG TTGGAGATGC CGCTGCACTT 
TCATGCCATC AGTTCGGTAT GTGCTGGAGA CTTTGGAGAG CATACATAGA GGAATGCATC 
AACAAGGAAA GTTGCCACGA CCTTACTCTG GATTCTTTAA AGCAAGATCT CCTGGAGATT 
TCTAAACTCT GCAGGAGCGA GGATGAAGAG AACAGCGATA GTGAATCAAC TACATCTGAA 
CTACCGGACA CTGAAGACCT AATACTTCAA GAGCTTCTTG ATGAGAGCAA AGAAATCAAT 
GTTGCTTTGA CAGAGGAAGG ATTATTTCCA GTTTTTCAAA ATCAGGAAGC ATTCAGTAGT 
GCAAATAACC AAGTTGCAGA AGTATATTAT GAAGAGCCTA TGTTTACTAG CTCTACTGTC 
CACAAACAGT GTGAGATAGT CAAAGAAGGT TATGACAGAG GATATGCAAA GCCACTTGAC 
GAGTCCACTC TGAGAATAGA CATCAAGGGT AGAGCGAACA TCGGAAGGGC ATTCCCTGGA 
GATAAAGTAA CGGTGGAGAT CCTGAGTGCA ACATCCCATC CTCCAATGGG AAGAGTGATC 
AACATCCTTG AAAGTGCAGA TACTGTCAAG GAATTTGTCT GCGCACTTGA AAGACATGAC 
AATCAAGTAA TGACGCCTAT AAACAAATGC ATCACCAAGA TCTTCACACC CTTTTGGAAA 
GACAAACCAA ACCACATTGC TGTAAGGAAT CCTGAGAGCC TCAAAGTAGA GAAGTTTATC 
AAAATCAACG AAGAAGCTCA CAGGAAATCT CTCTTTATTG TCAAAGTCCT TAGTTGGAGA 
AACGTTTTCA GATATCCTTT GGGGATTGTC GTAGGTGTGC TTCCTAAAAT AGCAACTTTA 
GATGATGGAT TGCATGTACT CAGCATTGAG TACCAGTTGA GAAGGACACT TTCCTCATCT 
CTGAGAAAGC AGATGCAATA CTTACAACGT CTTGATATTT TCGAAAAAGG ACGAAGAGAT 
TTTCGCATGC TCCCAACCTT CACAGTTGAT CCCGCACATT CGCAAGATCT CGATGATGCA 
ATAAGTTTGC AGGATTTTGG TGAGTACCTT GAAATAGGAG TTCACATTTC TGATGTTGCA 
AGCTTTGTGG CTAAAGATAG CGAACTGGAC AAATTTGCCA GACAGCTGTG TACTACATTC 
TACCCTCGTG AGAAGGAGCC AATATACATG TTTCCCAAAG AACTGAGCAC CAATTTGTTA 
AGTTTAATCC CTAACCAGGA CAGACGAGCA ATCTCATTGA TTATAGAAGT GGATGCACAG 
ACTAATCGCA TAAAGAGAAG ATTTATCTGC AAGTCTGTGA TTCACTCCAA CAGGAAATTT 
TCCTACGATG AAGTTGAGGA CATTCTTGAA AATGGTGAGC CGCTTTCCGA CCTGCTTGAA 
ATCTGTCTTG TAAAAGTGTC GAACTTTGCT GAGGTTCACC GAAGATACAG AAAGCAGGAT 
GACTGGTGCT ACAAGATGCC AGATGAGGAT GTGACCCTTG GAAGAAGGAG ATCTCACCAA 
ATGGTGGAAG AGCTGATGGT CATGTTTAAC CACATGGTTG CCGAACGTCT TTTATCTGAT 
GGTGCAACAA GAAGCAAAAC TCCATTGAGA TGCCAGGACA GGCCTCAAAC AGACAAACTC 
ATTGAGATTT TTGACAAAAA CGCAGCCTTG ATTCCGCTGT CTGTTCACTT CTCAAGTCAA 
GCTCATGAAC TGAATAAGCA AGGTTTGATC CAGCACAACC CTCAGACTGA CTCAGACACG 
TTCCCTGTGT TAAAATCAAT CCTCAGATGC CTGAAGTCAG CAGCAGAGCA GGAAAACATC 
TACAAAATAA TTGACTTTGT TGCAACTGAT GACATTCATC CTCAGCTTCT GCCTTTTGCT 
GTTGCATTTA GGCGGTTGTT TCACAAGGCG TGCATTCAGC GCTCAAACTC AACGCATGTC 
TCAAGAATAG GGCACTATGA TTTAGATCTT GACAGCTATA CATGGGCCTC ATCTCCAGTT 
CGCCGATACT TAGATGTAAT TGTGCAGCGC CTTCTTCATT CTGTGATTGA CAAGACAGAT 
GTTAAGTACA CAACTCTAGA CATTAACCTG TCTTGTTTGG AATTCTCCAG GAAAAATGAT 
CAACAGTCGG CCTATGAGAG GAAGGCAAAT GCTCTGCATT TCGCATTAAA ACTGTCAAGC 
CAAAGTGAAA GGAAGGTGGC TTATATTGTG GAGCTCAATC CATTAGGGAC AAACTTCAAA 
GTGTCCTTTC CACTTAACAG ATCCTCTATA TCAGAAAACT TAGACATTAT GAGCAGGGAT 
CTTCTGCTGG CTGTTCAGCC AGAGTTTGAT GTAACAAACA ACAGCATGAT CCTGAAATGG 
GAGCGAAGGA TATATTCATT CTCCAATTCT CACATCCATG CTGAAGTGAA GCAACAAAAC 
CCAAACTCAG TTATGGCCTA TGTCCCTGCA AAGACCTGGA AATGCATTGC AGATGCTATC 
AGAGAACAAA ACTGGAATGC CATTTTTCCT CTTATTGAGG AACTTCAACG CAACTCTGGC 
ATCAATGCAA GACAATTTCA CGAAACACTG AGTGCTTCAA AATGCACGAA AAATTATGAC 
CCCTTGCATG CTGAGCATTA TGCTGAACTG TATCTGAGAA TTAAGCAAGG TGAGGCGGTC 
GAAGTGCAGC TTGGTGCATC CACAGCTCGT GGTTTAATGA CTCCATCGAT TCAGCTGTTT 
GTTGTGCATC CAAAGTTTGA GATTTGCGTG GAGCATGTAA GAGATCCAGT CAAGTGTTTT 
TCTGAATACG CACTAAGCTC ATCAAGGAGT TCATACAACT TGTACAAGGA TTATCAGGCA 
ATATGGAGAC CTATGTGCGA GATGGAGTCA GCCTCCAGCG CTGTGGCTGA AAATGAGAGC 
ATTCTTATTG TAGATGTGCC CTTGAAATGG GAAAACTCCA ATCCGAAGAG GCTACAGGGG 
TTTTTTCTGC TCCCGCTTGA CAAGAAATCT CAGTGGTCAA TAGACTGTGA TCTCAGAAAC 
AGCTTCCTAT GCATTCGAAC GAGGATTCAG AAACAGAGTA GCCCATTTCT GGAGAGCGCT 
CAGAACACAG CGCTCATGGA TATTCCTTCT TTCACTTGGG TAGTTCATGG TGTGATTACA 
GAAGCTGAAG AAACAGAGTC CAAAGAATCA ACGAATCAAG AACCAAAGAG TTTGCGAATT 
GATTTTCTGA TTAATCACAT GCCCATGACA AACATTCCAG AACAAATATT CCACGAGAGG 
ACGAGATTTA CGCTGGAGCT GATTCCAAAG CTTCTGCCAG ACGTGCGCAA AGAAAATGCA 
ATTGACAGTC TAACCAAAGC CAACCAGCTT GTAAAAAACA TTGCCATGGG CAGGAGAATC 
ACTTCGGACA GAGGATGTAA AATACCAAAT CAGAATACAG GCAGGTTTGA GATCAACAGT 
TATCATCCTT CTGGGTTATC CCATCTAAAC AACAGCCAAA GTAAAGCAAT AAGAGAAGCA 
CTGACTAACC CATTCACACT TATCCAAGGA CCACCGGGGA CTGGAAAAAC TGTTGTTGGT 
GTCCATATTT TGTACTGGCT GCACAAGAAT ATCCAACAGC TCCAAGCTTC TGGTTCAAAG 
AAGAGAGCTA TTCTGTACTG TGGCCCGTCA AATAAATCTG TTGATGTTGT TGCAGGTCAT 
CTCCTACATC TCCGAGACCA TCTTAAACCT CTCCGGGTCT ACAGTGATCA GATGGAGATG 
TTGGAGTTTC CGTACCCAGG CTGCAATCTG AAGCTGTCGC GTAACTCTAA AAGAGGAGAA 
AAACCCAACA CTGAGCTCAG TTCCATTGCC TTACACCATC TGATTCGAAA GGAGGGCAAC 
CCATTCTCCA CACAAATACG TGCTTTTGAT ATGAAAGTCC AAAGAGGAGA TCAACTCACT 
GATGAAGAAA GAAAACTCTT CAGAGAAATC CTCAAAAAGG CTCGAAAGCA TGAATTATTG 
CACCATGACG TAATCTTATG CACGTGCACC GCAGCTTCAC ATCCAGCACT GGCGGAAACC 
CTTGACTTTA AGCAGATCAT CATTGATGAA TGTGCCATGG CCACTGAACC TGAAGCCTTT 
ATTCCTCTCG TGGCTCATAA ACCTGAGCAG ATTGTCCTTC TTGGCGATCA CAAACAGCTT 
CAACCAGTGG TCCACTGCGA GGTGGCGGAG CGATTAGGAA TGAGCAGATC TCTTTTTGAA 
CGTTACATGG AGAACGCACT CATGCTTGAC ACCCAGTACA GAATGCAAGA AGACATTTGT 
GCATTTCCAT CTAATGAGTT CTATGGGGGG AGATTACAAA CTGGAATACC AGCCAAATCA 
AAACTATTCC TTACTCAAAC AAAGCAAACC TGCATCGTTT TTGGTCATGT TGAAGGAAAG 
GAGAAAAGTC TGGTGGTTCG AACTGAACAA GGCAATGAAA ACTCAAAGGC AAATATGGAA 
GAGGCAGAGG AAGTGGTGCG GGTCGCCATT CTTTTGACTG AGGCTGGGAT CAAGACAAAG 
GACATTGCCA TTCTCACACC ATATAATGCT CAAGTGGCCA AAATTAGTGA GTCTCTGCAT 
AATAACCATA TACGAGACAT CACCGTCAAC ACCATCATGA GGAGTCAAGG TAGTGAATGG 
AGGTACGTCA TCATGTCAAC AGTGCGCTCT TGTCCCAAGT CTGAAATAGA GACACAACCA 
CCCAAATCCT GGATTATGAA GCGGCTTGGA TTCATCACCG ACCCTCATCA AGTAAACGTT 
GGCATTACCA GAGCACAAGA AGGGCTGTGC ATCATCGGAA ATGAGAACTT GCTGCGCTGT 
AGTTCCTTAT GGAGGAAACT TCTGAATCAT TATCAGGAGA AAGGATGTGT GGTGAATCCT 
GCCCGGCTCA TCCAAGTCCG ACAACCCCCA AGAAGAACTG CTAGAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELZ2 ( XM_003198847.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003198895.2 Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.

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CloneID	ODa12360
Clone ID Related Accession (Same CDS sequence)	XM_003198847.4 , XM_003198847.3
Accession Version	XM_003198847.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	helicase with zinc finger domain 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_003198847.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG CAAAGAACCA CTATAAACTT CAAGCATTAC TACAGACGTA CACACTGTGT 
GTGAGATGCG GGAGGTGCTG TAATAAACAC AATAAAATTT CATACTCCCT CAACAATATC 
AAGCACAGCT GCTCAGAAAA CATTGTACTG ATCAGACGGA AACAAGACGG CTCATACTGG 
AGGCCTGTTG CACCCTTGCC AAACTTCCCC AGACCAAAGA AGTATGTTGC TTGCTGGTAC 
TACACTAAAG ATCGTGGATG CACAAGGCAT GGAAATAATT GTCAATTTGC GAGCAGCTCG 
GAGGAAGCAG ACTTGTGGAA TGTCATGAAA GATGAAAAAC TGACCATTCC TCAGATTGTG 
AATGCGATCA AACAAAACCA AAGGACACCG CAGATAACAT TTCAGGAGAA ACGAGGACCG 
CATGACTGCA CGTTATGTCA GGTCCACTTC CCAACGGACG AGGACTTCAT GAACCACTGT 
TTCACCGTAA AGCACAGAAG ACGGATCTTT GAAGATGGCA ATAAGACCTG GAAGCACCGC 
GAGCCGCCAC CAACACACAA GGACCTGAAA CTGTGTGAAA GATCCCTGAT ATGTGAGTAT 
GGAGATAACT GCACAAAGGC GCACAGTCAA GAGGAACTTC AAGAATGGCA AACAAGAGTC 
AGAGCTGTAC GAAATAAAGC CCGAGATGCA GCCAGTCAGG GTCTACTCTC CTACCAGGAC 
AATCTGCTGG ACGAATACAG GCACAGCAAA GACAAGAAGA TGATTATGAC TGACACCATT 
CCTGATGTGT TCATCACCTG TGACCGAGAT TTAGATGCTG TCTATGTAAG GAAAAGGGGA 
ATACAGTGTA CATGGAATTT TACCATCATA TCTAAGGAGC CTCTTGAAGT TATTGCTTTA 
CTCAGGCGGG AACAGGGGGC AAAATTCAGC CTCAGCAAAA AACCAGATGA GGAACGGTCG 
TATTCCACGA GCGCTTGGTT TGAAAAGAGG ACAGAGCAAA CTGGGCATGA AATCCCAGTG 
TCATTCAGAT CTGACCAGCC TGGCCTGTAT GAGCAGTGGG TAGTGTTTGA CTTCAATACG 
AGACCTGTAC TTCGACAAAA ACTAAAGGTC AGAGTTGGAG AGGATGATTG TCAGGCAGCT 
CCAGCACCGG AGGCACTATT AGAAAACCCA GAAGCTGCCT GCAATCTTGA GCCTTGGAAT 
GAGGAAAGCA TGACAATCAT TCCTTTTGTT GAAACAGAAA AAGCACAGAG AGACCTACTA 
AACAGATACC AACTCATCAT GATGAACCAG CAGATGAACG GTTTGATTAA CCGTGAGAAT 
TATAAATGGA GCATGCACAA TTTTTTATAC CAGGAGGAGA AGGTAGTCGG TGAACTGCTT 
TCCAGATTAA GTCTACGAGG GACTGTGCAG CTGAAAAATG AGTTGTCTGA TGACAGGTTT 
GGAATGAAAT GGGCTCACCC GGGTGAACTT TTTGCTGCTG TGCCTCTGTC TCAGCCACTC 
ACACCTGATG CTCCTCAAGG TTTCATTGTG AAAAGACATG CCATCAGATC TCTTGTTCGT 
GTTGTGAAGA ACAATGAATA TTGTGGGCCT ATTTATGAAG CTGAGTTTCT GAAAGATGCT 
GCCTCCGAGA CACAAGTACA TCTCCAGCTT TCCAAACGCT GCTGTGCTGC CCTTAACCTG 
CAGAATGGAG AGGAATATGA GATGGAGGTC CAGTTTCAAG TCAATCGTCT GTGGTTCTGC 
GAGATGCACA AGGCAATTGA TGATCTCCCG AGGATGGACA AAGTGTTGCC TGATCTGAAA 
AATGCTCACC TCAGTATTTC TTTCCAGTCA GATGAAGGAG AGTTCAATGA GAAGCAGCAA 
GCAGCAATGG ACTTTGTCTT GGGTGTGTCA GACAACAGAA GCAGCATCCC ACCCTTGTTG 
ATTTATGGAC CTTTTGGGAC TGGCAAAACT CGAACCCTTG CAAAGATGGC ACAGGCCCTT 
GTACAGCAGC CACACAACAA AATCTTAATT TGCACACACA CAAACAGTTC TGCTGATCTT 
TATATAAAAG CTCATTTCCA CGAATATGTA AGGAATGGCC AGCTTCACGC CAGACCTCTG 
CGAATAAAGG CAATGGAAAT AAAGCTCGCA TCCACCGATT TCATCACTCT ACAGTACTGC 
CATTTATCAA GAGAAGCCAA TTGCTTTGAG TTTCCTGATA AAGCAGTTTT AGATTCCACA 
AGAATCGTCA TAACAACAAC ATCTCTGGCT CGTTTCTTCA ACGACATGAA GCTTCCTGAA 
AACTACTTCA GCCATATTCT GATTGATGAA GCGTCTCAGA TGTTGGAGTG TGAGGCTCTT 
ATGGCACTTA GCTTAGCGGG TAACAATACA CGTGTGGTTT TAGCAGGAGA TCACATGCAG 
ATGGGACCCA AGCTGTTCTC TGTGAGGCCA GACAAATGTT CTGAGCACAC CCTGCTCAAT 
CGCCTCTTCT ATCACTACCA AGCCGAAAAC AGCGATGTGG CCAAACAAAG CCGGATCATT 
TTCAATGAAA ACTACCGCTC CACAAAAGAC ATTGTGGATT TTGTATCAAC ACATTTTTAT 
GTTGGAAGGA GTGATGTGAT CAAGGCTAGA GGAAATGTGC CGTCTCATCC CCAACAACAT 
GCTTTGCAGT TTCATCATGT AAGAGGAGAA TGCTGTCTGG ACCCAATGAG CATGTCCTGG 
TTCAATGCAG AACAAATTGT AAGAGTCGTT CACATTCTGC AAGAAATAAT GAAGGAATGG 
CCTCAAGAGT GGGGTGCTGC AGATCCAGAA TCAGTCTGTG TCCTATCTCA AGGCAGACAG 
GTGTTGGAAA TCAGGTATAG ACTGCGTTTG CTTGGACTGC CTCGTTTCAC TGTAGAGAAT 
GCTGAAAATG TGCAAGGAAA GCAGTTCAGA GTAATTGTGA TTTCCACTGT GCACACCAAG 
GACAGCCTAT TGGAGACGGA CAGGACCTGT CTTGAGTTTT TCAATGACAT GCGAGTCCTG 
AACACCATCA TGACAAGAGC CCAATCACAA ATATTTGTTG TTGGAGATGC CGCTGCACTT 
TCATGCCATC AGTTCGGTAT GTGCTGGAGA CTTTGGAGAG CATACATAGA GGAATGCATC 
AACAAGGAAA GTTGCCACGA CCTTACTCTG GATTCTTTAA AGCAAGATCT CCTGGAGATT 
TCTAAACTCT GCAGGAGCGA GGATGAAGAG AACAGCGATA GTGAATCAAC TACATCTGAA 
CTACCGGACA CTGAAGACCT AATACTTCAA GAGCTTCTTG ATGAGAGCAA AGAAATCAAT 
GTTGCTTTGA CAGAGGAAGG ATTATTTCCA GTTTTTCAAA ATCAGGAAGC ATTCAGTAGT 
GCAAATAACC AAGTTGCAGA AGTATATTAT GAAGAGCCTA TGTTTACTAG CTCTACTGTC 
CACAAACAGT GTGAGATAGT CAAAGAAGGT TATGACAGAG GATATGCAAA GCCACTTGAC 
GAGTCCACTC TGAGAATAGA CATCAAGGGT AGAGCGAACA TCGGAAGGGC ATTCCCTGGA 
GATAAAGTAA CGGTGGAGAT CCTGAGTGCA ACATCCCATC CTCCAATGGG AAGAGTGATC 
AACATCCTTG AAAGTGCAGA TACTGTCAAG GAATTTGTCT GCGCACTTGA AAGACATGAC 
AATCAAGTAA TGACGCCTAT AAACAAATGC ATCACCAAGA TCTTCACACC CTTTTGGAAA 
GACAAACCAA ACCACATTGC TGTAAGGAAT CCTGAGAGCC TCAAAGTAGA GAAGTTTATC 
AAAATCAACG AAGAAGCTCA CAGGAAATCT CTCTTTATTG TCAAAGTCCT TAGTTGGAGA 
AACGTTTTCA GATATCCTTT GGGGATTGTC GTAGGTGTGC TTCCTAAAAT AGCAACTTTA 
GATGATGGAT TGCATGTACT CAGCATTGAG TACCAGTTGA GAAGGACACT TTCCTCATCT 
CTGAGAAAGC AGATGCAATA CTTACAACGT CTTGATATTT TCGAAAAAGG ACGAAGAGAT 
TTTCGCATGC TCCCAACCTT CACAGTTGAT CCCGCACATT CGCAAGATCT CGATGATGCA 
ATAAGTTTGC AGGATTTTGG TGAGTACCTT GAAATAGGAG TTCACATTTC TGATGTTGCA 
AGCTTTGTGG CTAAAGATAG CGAACTGGAC AAATTTGCCA GACAGCTGTG TACTACATTC 
TACCCTCGTG AGAAGGAGCC AATATACATG TTTCCCAAAG AACTGAGCAC CAATTTGTTA 
AGTTTAATCC CTAACCAGGA CAGACGAGCA ATCTCATTGA TTATAGAAGT GGATGCACAG 
ACTAATCGCA TAAAGAGAAG ATTTATCTGC AAGTCTGTGA TTCACTCCAA CAGGAAATTT 
TCCTACGATG AAGTTGAGGA CATTCTTGAA AATGGTGAGC CGCTTTCCGA CCTGCTTGAA 
ATCTGTCTTG TAAAAGTGTC GAACTTTGCT GAGGTTCACC GAAGATACAG AAAGCAGGAT 
GACTGGTGCT ACAAGATGCC AGATGAGGAT GTGACCCTTG GAAGAAGGAG ATCTCACCAA 
ATGGTGGAAG AGCTGATGGT CATGTTTAAC CACATGGTTG CCGAACGTCT TTTATCTGAT 
GGTGCAACAA GAAGCAAAAC TCCATTGAGA TGCCAGGACA GGCCTCAAAC AGACAAACTC 
ATTGAGATTT TTGACAAAAA CGCAGCCTTG ATTCCGCTGT CTGTTCACTT CTCAAGTCAA 
GCTCATGAAC TGAATAAGCA AGGTTTGATC CAGCACAACC CTCAGACTGA CTCAGACACG 
TTCCCTGTGT TAAAATCAAT CCTCAGATGC CTGAAGTCAG CAGCAGAGCA GGAAAACATC 
TACAAAATAA TTGACTTTGT TGCAACTGAT GACATTCATC CTCAGCTTCT GCCTTTTGCT 
GTTGCATTTA GGCGGTTGTT TCACAAGGCG TGCATTCAGC GCTCAAACTC AACGCATGTC 
TCAAGAATAG GGCACTATGA TTTAGATCTT GACAGCTATA CATGGGCCTC ATCTCCAGTT 
CGCCGATACT TAGATGTAAT TGTGCAGCGC CTTCTTCATT CTGTGATTGA CAAGACAGAT 
GTTAAGTACA CAACTCTAGA CATTAACCTG TCTTGTTTGG AATTCTCCAG GAAAAATGAT 
CAACAGTCGG CCTATGAGAG GAAGGCAAAT GCTCTGCATT TCGCATTAAA ACTGTCAAGC 
CAAAGTGAAA GGAAGGTGGC TTATATTGTG GAGCTCAATC CATTAGGGAC AAACTTCAAA 
GTGTCCTTTC CACTTAACAG ATCCTCTATA TCAGAAAACT TAGACATTAT GAGCAGGGAT 
CTTCTGCTGG CTGTTCAGCC AGAGTTTGAT GTAACAAACA ACAGCATGAT CCTGAAATGG 
GAGCGAAGGA TATATTCATT CTCCAATTCT CACATCCATG CTGAAGTGAA GCAACAAAAC 
CCAAACTCAG TTATGGCCTA TGTCCCTGCA AAGACCTGGA AATGCATTGC AGATGCTATC 
AGAGAACAAA ACTGGAATGC CATTTTTCCT CTTATTGAGG AACTTCAACG CAACTCTGGC 
ATCAATGCAA GACAATTTCA CGAAACACTG AGTGCTTCAA AATGCACGAA AAATTATGAC 
CCCTTGCATG CTGAGCATTA TGCTGAACTG TATCTGAGAA TTAAGCAAGG TGAGGCGGTC 
GAAGTGCAGC TTGGTGCATC CACAGCTCGT GGTTTAATGA CTCCATCGAT TCAGCTGTTT 
GTTGTGCATC CAAAGTTTGA GATTTGCGTG GAGCATGTAA GAGATCCAGT CAAGTGTTTT 
TCTGAATACG CACTAAGCTC ATCAAGGAGT TCATACAACT TGTACAAGGA TTATCAGGCA 
ATATGGAGAC CTATGTGCGA GATGGAGTCA GCCTCCAGCG CTGTGGCTGA AAATGAGAGC 
ATTCTTATTG TAGATGTGCC CTTGAAATGG GAAAACTCCA ATCCGAAGAG GCTACAGGGG 
TTTTTTCTGC TCCCGCTTGA CAAGAAATCT CAGTGGTCAA TAGACTGTGA TCTCAGAAAC 
AGCTTCCTAT GCATTCGAAC GAGGATTCAG AAACAGAGTA GCCCATTTCT GGAGAGCGCT 
CAGAACACAG CGCTCATGGA TATTCCTTCT TTCACTTGGG TAGTTCATGG TGTGATTACA 
GAAGCTGAAG AAACAGAGTC CAAAGAATCA ACGAATCAAG AACCAAAGAG TTTGCGAATT 
GATTTTCTGA TTAATCACAT GCCCATGACA AACATTCCAG AACAAATATT CCACGAGAGG 
ACGAGATTTA CGCTGGAGCT GATTCCAAAG CTTCTGCCAG ACGTGCGCAA AGAAAATGCA 
ATTGACAGTC TAACCAAAGC CAACCAGCTT GTAAAAAACA TTGCCATGGG CAGGAGAATC 
ACTTCGGACA GAGGATGTAA AATACCAAAT CAGAATACAG GCAGGTTTGA GATCAACAGT 
TATCATCCTT CTGGGTTATC CCATCTAAAC AACAGCCAAA GTAAAGCAAT AAGAGAAGCA 
CTGACTAACC CATTCACACT TATCCAAGGA CCACCGGGGA CTGGAAAAAC TGTTGTTGGT 
GTCCATATTT TGTACTGGCT GCACAAGAAT ATCCAACAGC TCCAAGCTTC TGGTTCAAAG 
AAGAGAGCTA TTCTGTACTG TGGCCCGTCA AATAAATCTG TTGATGTTGT TGCAGGTCAT 
CTCCTACATC TCCGAGACCA TCTTAAACCT CTCCGGGTCT ACAGTGATCA GATGGAGATG 
TTGGAGTTTC CGTACCCAGG CTGCAATCTG AAGCTGTCGC GTAACTCTAA AAGAGGAGAA 
AAACCCAACA CTGAGCTCAG TTCCATTGCC TTACACCATC TGATTCGAAA GGAGGGCAAC 
CCATTCTCCA CACAAATACG TGCTTTTGAT ATGAAAGTCC AAAGAGGAGA TCAACTCACT 
GATGAAGAAA GAAAACTCTT CAGAGAAATC CTCAAAAAGG CTCGAAAGCA TGAATTATTG 
CACCATGACG TAATCTTATG CACGTGCACC GCAGCTTCAC ATCCAGCACT GGCGGAAACC 
CTTGACTTTA AGCAGATCAT CATTGATGAA TGTGCCATGG CCACTGAACC TGAAGCCTTT 
ATTCCTCTCG TGGCTCATAA ACCTGAGCAG ATTGTCCTTC TTGGCGATCA CAAACAGCTT 
CAACCAGTGG TCCACTGCGA GGTGGCGGAG CGATTAGGAA TGAGCAGATC TCTTTTTGAA 
CGTTACATGG AGAACGCACT CATGCTTGAC ACCCAGTACA GAATGCAAGA AGACATTTGT 
GCATTTCCAT CTAATGAGTT CTATGGGGGG AGATTACAAA CTGGAATACC AGCCAAATCA 
AAACTATTCC TTACTCAAAC AAAGCAAACC TGCATCGTTT TTGGTCATGT TGAAGGAAAG 
GAGAAAAGTC TGGTGGTTCG AACTGAACAA GGCAATGAAA ACTCAAAGGC AAATATGGAA 
GAGGCAGAGG AAGTGGTGCG GGTCGCCATT CTTTTGACTG AGGCTGGGAT CAAGACAAAG 
GACATTGCCA TTCTCACACC ATATAATGCT CAAGTGGCCA AAATTAGTGA GTCTCTGCAT 
AATAACCATA TACGAGACAT CACCGTCAAC ACCATCATGA GGAGTCAAGG TAGTGAATGG 
AGGTACGTCA TCATGTCAAC AGTGCGCTCT TGTCCCAAGT CTGAAATAGA GACACAACCA 
CCCAAATCCT GGATTATGAA GCGGCTTGGA TTCATCACCG ACCCTCATCA AGTAAACGTT 
GGCATTACCA GAGCACAAGA AGGGCTGTGC ATCATCGGAA ATGAGAACTT GCTGCGCTGT 
AGTTCCTTAT GGAGGAAACT TCTGAATCAT TATCAGGAGA AAGGATGTGT GGTGAATCCT 
GCCCGGCTCA TCCAAGTCCG ACAACCCCCA AGAAGAACTG CTAGAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198895.2
CDS	103..8073
Translation	MALAKNHYKLQALLQTYTLCVRCGRCCNKHNKISYSLNNIKHSCSENIVLIRRKQDGSYWRPVAPLPNFPRPKKYVACWYYTKDRGCTRHGNNCQFASSSEEADLWNVMKDEKLTIPQIVNAIKQNQRTPQITFQEKRGPHDCTLCQVHFPTDEDFMNHCFTVKHRRRIFEDGNKTWKHREPPPTHKDLKLCERSLICEYGDNCTKAHSQEELQEWQTRVRAVRNKARDAASQGLLSYQDNLLDEYRHSKDKKMIMTDTIPDVFITCDRDLDAVYVRKRGIQCTWNFTIISKEPLEVIALLRREQGAKFSLSKKPDEERSYSTSAWFEKRTEQTGHEIPVSFRSDQPGLYEQWVVFDFNTRPVLRQKLKVRVGEDDCQAAPAPEALLENPEAACNLEPWNEESMTIIPFVETEKAQRDLLNRYQLIMMNQQMNGLINRENYKWSMHNFLYQEEKVVGELLSRLSLRGTVQLKNELSDDRFGMKWAHPGELFAAVPLSQPLTPDAPQGFIVKRHAIRSLVRVVKNNEYCGPIYEAEFLKDAASETQVHLQLSKRCCAALNLQNGEEYEMEVQFQVNRLWFCEMHKAIDDLPRMDKVLPDLKNAHLSISFQSDEGEFNEKQQAAMDFVLGVSDNRSSIPPLLIYGPFGTGKTRTLAKMAQALVQQPHNKILICTHTNSSADLYIKAHFHEYVRNGQLHARPLRIKAMEIKLASTDFITLQYCHLSREANCFEFPDKAVLDSTRIVITTTSLARFFNDMKLPENYFSHILIDEASQMLECEALMALSLAGNNTRVVLAGDHMQMGPKLFSVRPDKCSEHTLLNRLFYHYQAENSDVAKQSRIIFNENYRSTKDIVDFVSTHFYVGRSDVIKARGNVPSHPQQHALQFHHVRGECCLDPMSMSWFNAEQIVRVVHILQEIMKEWPQEWGAADPESVCVLSQGRQVLEIRYRLRLLGLPRFTVENAENVQGKQFRVIVISTVHTKDSLLETDRTCLEFFNDMRVLNTIMTRAQSQIFVVGDAAALSCHQFGMCWRLWRAYIEECINKESCHDLTLDSLKQDLLEISKLCRSEDEENSDSESTTSELPDTEDLILQELLDESKEINVALTEEGLFPVFQNQEAFSSANNQVAEVYYEEPMFTSSTVHKQCEIVKEGYDRGYAKPLDESTLRIDIKGRANIGRAFPGDKVTVEILSATSHPPMGRVINILESADTVKEFVCALERHDNQVMTPINKCITKIFTPFWKDKPNHIAVRNPESLKVEKFIKINEEAHRKSLFIVKVLSWRNVFRYPLGIVVGVLPKIATLDDGLHVLSIEYQLRRTLSSSLRKQMQYLQRLDIFEKGRRDFRMLPTFTVDPAHSQDLDDAISLQDFGEYLEIGVHISDVASFVAKDSELDKFARQLCTTFYPREKEPIYMFPKELSTNLLSLIPNQDRRAISLIIEVDAQTNRIKRRFICKSVIHSNRKFSYDEVEDILENGEPLSDLLEICLVKVSNFAEVHRRYRKQDDWCYKMPDEDVTLGRRRSHQMVEELMVMFNHMVAERLLSDGATRSKTPLRCQDRPQTDKLIEIFDKNAALIPLSVHFSSQAHELNKQGLIQHNPQTDSDTFPVLKSILRCLKSAAEQENIYKIIDFVATDDIHPQLLPFAVAFRRLFHKACIQRSNSTHVSRIGHYDLDLDSYTWASSPVRRYLDVIVQRLLHSVIDKTDVKYTTLDINLSCLEFSRKNDQQSAYERKANALHFALKLSSQSERKVAYIVELNPLGTNFKVSFPLNRSSISENLDIMSRDLLLAVQPEFDVTNNSMILKWERRIYSFSNSHIHAEVKQQNPNSVMAYVPAKTWKCIADAIREQNWNAIFPLIEELQRNSGINARQFHETLSASKCTKNYDPLHAEHYAELYLRIKQGEAVEVQLGASTARGLMTPSIQLFVVHPKFEICVEHVRDPVKCFSEYALSSSRSSYNLYKDYQAIWRPMCEMESASSAVAENESILIVDVPLKWENSNPKRLQGFFLLPLDKKSQWSIDCDLRNSFLCIRTRIQKQSSPFLESAQNTALMDIPSFTWVVHGVITEAEETESKESTNQEPKSLRIDFLINHMPMTNIPEQIFHERTRFTLELIPKLLPDVRKENAIDSLTKANQLVKNIAMGRRITSDRGCKIPNQNTGRFEINSYHPSGLSHLNNSQSKAIREALTNPFTLIQGPPGTGKTVVGVHILYWLHKNIQQLQASGSKKRAILYCGPSNKSVDVVAGHLLHLRDHLKPLRVYSDQMEMLEFPYPGCNLKLSRNSKRGEKPNTELSSIALHHLIRKEGNPFSTQIRAFDMKVQRGDQLTDEERKLFREILKKARKHELLHHDVILCTCTAASHPALAETLDFKQIIIDECAMATEPEAFIPLVAHKPEQIVLLGDHKQLQPVVHCEVAERLGMSRSLFERYMENALMLDTQYRMQEDICAFPSNEFYGGRLQTGIPAKSKLFLTQTKQTCIVFGHVEGKEKSLVVRTEQGNENSKANMEEAEEVVRVAILLTEAGIKTKDIAILTPYNAQVAKISESLHNNHIRDITVNTIMRSQGSEWRYVIMSTVRSCPKSEIETQPPKSWIMKRLGFITDPHQVNVGITRAQEGLCIIGNENLLRCSSLWRKLLNHYQEKGCVVNPARLIQVRQPPRRTARR

Target ORF information:

RefSeq Version	XM_003198847.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio helicase with zinc finger 2, transcriptional coactivator (helz2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198847.3

ORF Insert Sequence:

ATGGCTTTAG CAAAGAACCA CTATAAACTT CAAGCATTAC TACAGACGTA CACACTGTGT 
GTGAGATGCG GGAGGTGCTG TAATAAACAC AATAAAATTT CATACTCCCT CAACAATATC 
AAGCACAGCT GCTCAGAAAA CATTGTACTG ATCAGACGGA AACAAGACGG CTCATACTGG 
AGGCCTGTTG CACCCTTGCC AAACTTCCCC AGACCAAAGA AGTATGTTGC TTGCTGGTAC 
TACACTAAAG ATCGTGGATG CACAAGGCAT GGAAATAATT GTCAATTTGC GAGCAGCTCG 
GAGGAAGCAG ACTTGTGGAA TGTCATGAAA GATGAAAAAC TGACCATTCC TCAGATTGTG 
AATGCGATCA AACAAAACCA AAGGACACCG CAGATAACAT TTCAGGAGAA ACGAGGACCG 
CATGACTGCA CGTTATGTCA GGTCCACTTC CCAACGGACG AGGACTTCAT GAACCACTGT 
TTCACCGTAA AGCACAGAAG ACGGATCTTT GAAGATGGCA ATAAGACCTG GAAGCACCGC 
GAGCCGCCAC CAACACACAA GGACCTGAAA CTGTGTGAAA GATCCCTGAT ATGTGAGTAT 
GGAGATAACT GCACAAAGGC GCACAGTCAA GAGGAACTTC AAGAATGGCA AACAAGAGTC 
AGAGCTGTAC GAAATAAAGC CCGAGATGCA GCCAGTCAGG GTCTACTCTC CTACCAGGAC 
AATCTGCTGG ACGAATACAG GCACAGCAAA GACAAGAAGA TGATTATGAC TGACACCATT 
CCTGATGTGT TCATCACCTG TGACCGAGAT TTAGATGCTG TCTATGTAAG GAAAAGGGGA 
ATACAGTGTA CATGGAATTT TACCATCATA TCTAAGGAGC CTCTTGAAGT TATTGCTTTA 
CTCAGGCGGG AACAGGGGGC AAAATTCAGC CTCAGCAAAA AACCAGATGA GGAACGGTCG 
TATTCCACGA GCGCTTGGTT TGAAAAGAGG ACAGAGCAAA CTGGGCATGA AATCCCAGTG 
TCATTCAGAT CTGACCAGCC TGGCCTGTAT GAGCAGTGGG TAGTGTTTGA CTTCAATACG 
AGACCTGTAC TTCGACAAAA ACTAAAGGTC AGAGTTGGAG AGGATGATTG TCAGGCAGCT 
CCAGCACCGG AGGCACTATT AGAAAACCCA GAAGCTGCCT GCAATCTTGA GCCTTGGAAT 
GAGGAAAGCA TGACAATCAT TCCTTTTGTT GAAACAGAAA AAGCACAGAG AGACCTACTA 
AACAGATACC AACTCATCAT GATGAACCAG CAGATGAACG GTTTGATTAA CCGTGAGAAT 
TATAAATGGA GCATGCACAA TTTTTTATAC CAGGAGGAGA AGGTAGTCGG TGAACTGCTT 
TCCAGATTAA GTCTACGAGG GACTGTGCAG CTGAAAAATG AGTTGTCTGA TGACAGGTTT 
GGAATGAAAT GGGCTCACCC GGGTGAACTT TTTGCTGCTG TGCCTCTGTC TCAGCCACTC 
ACACCTGATG CTCCTCAAGG TTTCATTGTG AAAAGACATG CCATCAGATC TCTTGTTCGT 
GTTGTGAAGA ACAATGAATA TTGTGGGCCT ATTTATGAAG CTGAGTTTCT GAAAGATGCT 
GCCTCCGAGA CACAAGTACA TCTCCAGCTT TCCAAACGCT GCTGTGCTGC CCTTAACCTG 
CAGAATGGAG AGGAATATGA GATGGAGGTC CAGTTTCAAG TCAATCGTCT GTGGTTCTGC 
GAGATGCACA AGGCAATTGA TGATCTCCCG AGGATGGACA AAGTGTTGCC TGATCTGAAA 
AATGCTCACC TCAGTATTTC TTTCCAGTCA GATGAAGGAG AGTTCAATGA GAAGCAGCAA 
GCAGCAATGG ACTTTGTCTT GGGTGTGTCA GACAACAGAA GCAGCATCCC ACCCTTGTTG 
ATTTATGGAC CTTTTGGGAC TGGCAAAACT CGAACCCTTG CAAAGATGGC ACAGGCCCTT 
GTACAGCAGC CACACAACAA AATCTTAATT TGCACACACA CAAACAGTTC TGCTGATCTT 
TATATAAAAG CTCATTTCCA CGAATATGTA AGGAATGGCC AGCTTCACGC CAGACCTCTG 
CGAATAAAGG CAATGGAAAT AAAGCTCGCA TCCACCGATT TCATCACTCT ACAGTACTGC 
CATTTATCAA GAGAAGCCAA TTGCTTTGAG TTTCCTGATA AAGCAGTTTT AGATTCCACA 
AGAATCGTCA TAACAACAAC ATCTCTGGCT CGTTTCTTCA ACGACATGAA GCTTCCTGAA 
AACTACTTCA GCCATATTCT GATTGATGAA GCGTCTCAGA TGTTGGAGTG TGAGGCTCTT 
ATGGCACTTA GCTTAGCGGG TAACAATACA CGTGTGGTTT TAGCAGGAGA TCACATGCAG 
ATGGGACCCA AGCTGTTCTC TGTGAGGCCA GACAAATGTT CTGAGCACAC CCTGCTCAAT 
CGCCTCTTCT ATCACTACCA AGCCGAAAAC AGCGATGTGG CCAAACAAAG CCGGATCATT 
TTCAATGAAA ACTACCGCTC CACAAAAGAC ATTGTGGATT TTGTATCAAC ACATTTTTAT 
GTTGGAAGGA GTGATGTGAT CAAGGCTAGA GGAAATGTGC CGTCTCATCC CCAACAACAT 
GCTTTGCAGT TTCATCATGT AAGAGGAGAA TGCTGTCTGG ACCCAATGAG CATGTCCTGG 
TTCAATGCAG AACAAATTGT AAGAGTCGTT CACATTCTGC AAGAAATAAT GAAGGAATGG 
CCTCAAGAGT GGGGTGCTGC AGATCCAGAA TCAGTCTGTG TCCTATCTCA AGGCAGACAG 
GTGTTGGAAA TCAGGTATAG ACTGCGTTTG CTTGGACTGC CTCGTTTCAC TGTAGAGAAT 
GCTGAAAATG TGCAAGGAAA GCAGTTCAGA GTAATTGTGA TTTCCACTGT GCACACCAAG 
GACAGCCTAT TGGAGACGGA CAGGACCTGT CTTGAGTTTT TCAATGACAT GCGAGTCCTG 
AACACCATCA TGACAAGAGC CCAATCACAA ATATTTGTTG TTGGAGATGC CGCTGCACTT 
TCATGCCATC AGTTCGGTAT GTGCTGGAGA CTTTGGAGAG CATACATAGA GGAATGCATC 
AACAAGGAAA GTTGCCACGA CCTTACTCTG GATTCTTTAA AGCAAGATCT CCTGGAGATT 
TCTAAACTCT GCAGGAGCGA GGATGAAGAG AACAGCGATA GTGAATCAAC TACATCTGAA 
CTACCGGACA CTGAAGACCT AATACTTCAA GAGCTTCTTG ATGAGAGCAA AGAAATCAAT 
GTTGCTTTGA CAGAGGAAGG ATTATTTCCA GTTTTTCAAA ATCAGGAAGC ATTCAGTAGT 
GCAAATAACC AAGTTGCAGA AGTATATTAT GAAGAGCCTA TGTTTACTAG CTCTACTGTC 
CACAAACAGT GTGAGATAGT CAAAGAAGGT TATGACAGAG GATATGCAAA GCCACTTGAC 
GAGTCCACTC TGAGAATAGA CATCAAGGGT AGAGCGAACA TCGGAAGGGC ATTCCCTGGA 
GATAAAGTAA CGGTGGAGAT CCTGAGTGCA ACATCCCATC CTCCAATGGG AAGAGTGATC 
AACATCCTTG AAAGTGCAGA TACTGTCAAG GAATTTGTCT GCGCACTTGA AAGACATGAC 
AATCAAGTAA TGACGCCTAT AAACAAATGC ATCACCAAGA TCTTCACACC CTTTTGGAAA 
GACAAACCAA ACCACATTGC TGTAAGGAAT CCTGAGAGCC TCAAAGTAGA GAAGTTTATC 
AAAATCAACG AAGAAGCTCA CAGGAAATCT CTCTTTATTG TCAAAGTCCT TAGTTGGAGA 
AACGTTTTCA GATATCCTTT GGGGATTGTC GTAGGTGTGC TTCCTAAAAT AGCAACTTTA 
GATGATGGAT TGCATGTACT CAGCATTGAG TACCAGTTGA GAAGGACACT TTCCTCATCT 
CTGAGAAAGC AGATGCAATA CTTACAACGT CTTGATATTT TCGAAAAAGG ACGAAGAGAT 
TTTCGCATGC TCCCAACCTT CACAGTTGAT CCCGCACATT CGCAAGATCT CGATGATGCA 
ATAAGTTTGC AGGATTTTGG TGAGTACCTT GAAATAGGAG TTCACATTTC TGATGTTGCA 
AGCTTTGTGG CTAAAGATAG CGAACTGGAC AAATTTGCCA GACAGCTGTG TACTACATTC 
TACCCTCGTG AGAAGGAGCC AATATACATG TTTCCCAAAG AACTGAGCAC CAATTTGTTA 
AGTTTAATCC CTAACCAGGA CAGACGAGCA ATCTCATTGA TTATAGAAGT GGATGCACAG 
ACTAATCGCA TAAAGAGAAG ATTTATCTGC AAGTCTGTGA TTCACTCCAA CAGGAAATTT 
TCCTACGATG AAGTTGAGGA CATTCTTGAA AATGGTGAGC CGCTTTCCGA CCTGCTTGAA 
ATCTGTCTTG TAAAAGTGTC GAACTTTGCT GAGGTTCACC GAAGATACAG AAAGCAGGAT 
GACTGGTGCT ACAAGATGCC AGATGAGGAT GTGACCCTTG GAAGAAGGAG ATCTCACCAA 
ATGGTGGAAG AGCTGATGGT CATGTTTAAC CACATGGTTG CCGAACGTCT TTTATCTGAT 
GGTGCAACAA GAAGCAAAAC TCCATTGAGA TGCCAGGACA GGCCTCAAAC AGACAAACTC 
ATTGAGATTT TTGACAAAAA CGCAGCCTTG ATTCCGCTGT CTGTTCACTT CTCAAGTCAA 
GCTCATGAAC TGAATAAGCA AGGTTTGATC CAGCACAACC CTCAGACTGA CTCAGACACG 
TTCCCTGTGT TAAAATCAAT CCTCAGATGC CTGAAGTCAG CAGCAGAGCA GGAAAACATC 
TACAAAATAA TTGACTTTGT TGCAACTGAT GACATTCATC CTCAGCTTCT GCCTTTTGCT 
GTTGCATTTA GGCGGTTGTT TCACAAGGCG TGCATTCAGC GCTCAAACTC AACGCATGTC 
TCAAGAATAG GGCACTATGA TTTAGATCTT GACAGCTATA CATGGGCCTC ATCTCCAGTT 
CGCCGATACT TAGATGTAAT TGTGCAGCGC CTTCTTCATT CTGTGATTGA CAAGACAGAT 
GTTAAGTACA CAACTCTAGA CATTAACCTG TCTTGTTTGG AATTCTCCAG GAAAAATGAT 
CAACAGTCGG CCTATGAGAG GAAGGCAAAT GCTCTGCATT TCGCATTAAA ACTGTCAAGC 
CAAAGTGAAA GGAAGGTGGC TTATATTGTG GAGCTCAATC CATTAGGGAC AAACTTCAAA 
GTGTCCTTTC CACTTAACAG ATCCTCTATA TCAGAAAACT TAGACATTAT GAGCAGGGAT 
CTTCTGCTGG CTGTTCAGCC AGAGTTTGAT GTAACAAACA ACAGCATGAT CCTGAAATGG 
GAGCGAAGGA TATATTCATT CTCCAATTCT CACATCCATG CTGAAGTGAA GCAACAAAAC 
CCAAACTCAG TTATGGCCTA TGTCCCTGCA AAGACCTGGA AATGCATTGC AGATGCTATC 
AGAGAACAAA ACTGGAATGC CATTTTTCCT CTTATTGAGG AACTTCAACG CAACTCTGGC 
ATCAATGCAA GACAATTTCA CGAAACACTG AGTGCTTCAA AATGCACGAA AAATTATGAC 
CCCTTGCATG CTGAGCATTA TGCTGAACTG TATCTGAGAA TTAAGCAAGG TGAGGCGGTC 
GAAGTGCAGC TTGGTGCATC CACAGCTCGT GGTTTAATGA CTCCATCGAT TCAGCTGTTT 
GTTGTGCATC CAAAGTTTGA GATTTGCGTG GAGCATGTAA GAGATCCAGT CAAGTGTTTT 
TCTGAATACG CACTAAGCTC ATCAAGGAGT TCATACAACT TGTACAAGGA TTATCAGGCA 
ATATGGAGAC CTATGTGCGA GATGGAGTCA GCCTCCAGCG CTGTGGCTGA AAATGAGAGC 
ATTCTTATTG TAGATGTGCC CTTGAAATGG GAAAACTCCA ATCCGAAGAG GCTACAGGGG 
TTTTTTCTGC TCCCGCTTGA CAAGAAATCT CAGTGGTCAA TAGACTGTGA TCTCAGAAAC 
AGCTTCCTAT GCATTCGAAC GAGGATTCAG AAACAGAGTA GCCCATTTCT GGAGAGCGCT 
CAGAACACAG CGCTCATGGA TATTCCTTCT TTCACTTGGG TAGTTCATGG TGTGATTACA 
GAAGCTGAAG AAACAGAGTC CAAAGAATCA ACGAATCAAG AACCAAAGAG TTTGCGAATT 
GATTTTCTGA TTAATCACAT GCCCATGACA AACATTCCAG AACAAATATT CCACGAGAGG 
ACGAGATTTA CGCTGGAGCT GATTCCAAAG CTTCTGCCAG ACGTGCGCAA AGAAAATGCA 
ATTGACAGTC TAACCAAAGC CAACCAGCTT GTAAAAAACA TTGCCATGGG CAGGAGAATC 
ACTTCGGACA GAGGATGTAA AATACCAAAT CAGAATACAG GCAGGTTTGA GATCAACAGT 
TATCATCCTT CTGGGTTATC CCATCTAAAC AACAGCCAAA GTAAAGCAAT AAGAGAAGCA 
CTGACTAACC CATTCACACT TATCCAAGGA CCACCGGGGA CTGGAAAAAC TGTTGTTGGT 
GTCCATATTT TGTACTGGCT GCACAAGAAT ATCCAACAGC TCCAAGCTTC TGGTTCAAAG 
AAGAGAGCTA TTCTGTACTG TGGCCCGTCA AATAAATCTG TTGATGTTGT TGCAGGTCAT 
CTCCTACATC TCCGAGACCA TCTTAAACCT CTCCGGGTCT ACAGTGATCA GATGGAGATG 
TTGGAGTTTC CGTACCCAGG CTGCAATCTG AAGCTGTCGC GTAACTCTAA AAGAGGAGAA 
AAACCCAACA CTGAGCTCAG TTCCATTGCC TTACACCATC TGATTCGAAA GGAGGGCAAC 
CCATTCTCCA CACAAATACG TGCTTTTGAT ATGAAAGTCC AAAGAGGAGA TCAACTCACT 
GATGAAGAAA GAAAACTCTT CAGAGAAATC CTCAAAAAGG CTCGAAAGCA TGAATTATTG 
CACCATGACG TAATCTTATG CACGTGCACC GCAGCTTCAC ATCCAGCACT GGCGGAAACC 
CTTGACTTTA AGCAGATCAT CATTGATGAA TGTGCCATGG CCACTGAACC TGAAGCCTTT 
ATTCCTCTCG TGGCTCATAA ACCTGAGCAG ATTGTCCTTC TTGGCGATCA CAAACAGCTT 
CAACCAGTGG TCCACTGCGA GGTGGCGGAG CGATTAGGAA TGAGCAGATC TCTTTTTGAA 
CGTTACATGG AGAACGCACT CATGCTTGAC ACCCAGTACA GAATGCAAGA AGACATTTGT 
GCATTTCCAT CTAATGAGTT CTATGGGGGG AGATTACAAA CTGGAATACC AGCCAAATCA 
AAACTATTCC TTACTCAAAC AAAGCAAACC TGCATCGTTT TTGGTCATGT TGAAGGAAAG 
GAGAAAAGTC TGGTGGTTCG AACTGAACAA GGCAATGAAA ACTCAAAGGC AAATATGGAA 
GAGGCAGAGG AAGTGGTGCG GGTCGCCATT CTTTTGACTG AGGCTGGGAT CAAGACAAAG 
GACATTGCCA TTCTCACACC ATATAATGCT CAAGTGGCCA AAATTAGTGA GTCTCTGCAT 
AATAACCATA TACGAGACAT CACCGTCAAC ACCATCATGA GGAGTCAAGG TAGTGAATGG 
AGGTACGTCA TCATGTCAAC AGTGCGCTCT TGTCCCAAGT CTGAAATAGA GACACAACCA 
CCCAAATCCT GGATTATGAA GCGGCTTGGA TTCATCACCG ACCCTCATCA AGTAAACGTT 
GGCATTACCA GAGCACAAGA AGGGCTGTGC ATCATCGGAA ATGAGAACTT GCTGCGCTGT 
AGTTCCTTAT GGAGGAAACT TCTGAATCAT TATCAGGAGA AAGGATGTGT GGTGAATCCT 
GCCCGGCTCA TCCAAGTCCG ACAACCCCCA AGAAGAACTG CTAGAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HELZ2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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