DENND4C cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND4C
Entrez Gene ID	558626
Full Name	DENN/MADD domain containing 4C
General protein information	Preferred Names DENN/MADD domain containing 4C Names DENN domain-containing protein 4C unm_sa1910
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001919955.7	XP_001919990.2	DENN domain-containing protein 4C isoform X2
XM_005166553.3	XP_005166610.1	DENN domain-containing protein 4C isoform X1
XM_005166553.4	XP_005166610.1	DENN domain-containing protein 4C isoform X1
XM_001919955.6	XP_001919990.2	DENN domain-containing protein 4C isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DENND4C	XP_005626722.1
Macaca mulatta (Rhesus monkey)	LOC712447	XP_001101451.2
Gallus gallus (chicken)	DENND4C	XP_004949183.1
Mus musculus (house mouse)	Dennd4c	NP_908976.1
Rattus norvegicus (Norway rat)	RGD1308489	XP_006238437.1
Xenopus tropicalis (tropical clawed frog)	dennd4c	XP_004910919.1
Pan troglodytes (chimpanzee)	DENND4C	XP_003312065.1
Bos taurus (cattle)	DENND4C	XP_002689611.3
Danio rerio (zebrafish)	dennd4c	XP_005166610.1
Homo sapiens (human)	DENND4C	NP_060395.5

Gene Ontology

The following DENND4C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND4C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13591	XM_005166553.4 Latest version!	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa13592	XM_001919955.7 Latest version!	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa13591	XM_005166553.3	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa13592	XM_001919955.6	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND4C ( XM_005166553.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166610.1 Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

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CloneID	ODa13591
Clone ID Related Accession (Same CDS sequence)	XM_005166553.3 , XM_005166553.4
Accession Version	XM_005166553.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 4C isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GCAGGTGTTT GCACTGGTAA GCTCTTCATG 
TCCCGTAAAA AGAGTGAAGA TGTGGCCTTG TCTGTGGAGG ATGCTACTGT AATCCCAGAT 
TCACAGCTTC CGCAGTCTGG ACAGCAGCAA AGGGTAAAGG CCTTCATGGA GCGCAGCTGC 
AGTTTTAGTG CAGAGAGCAA AGCTGGCATG CTACAGAAAA AAAGCAGTCT GGAGCTCAGT 
GCTGATCACA TGGGCGCAGA TGCAAAAATT CTTGCAGCAG TGTTCTCCGG CAGCCAGACT 
CCTCCTCCTT TTGCAAGCAT TGCTCCGTTT AAAGAGTTCA AGGTGGAGGC TACACTAAGC 
CCTGCCCTCC AGGAGGAAGA ACAAGAGGAA AAGCAGGAGA CCAAACATGA ATCCTTCACT 
TCTGCAGCAG AGGGAGTGGA GAAAAGTATA AAGCGGGATA ATGCAGCAGC AGAATCCAAT 
AGCAGTGAAC GAGTTGGGCG AACCACAGGG AGTGCCGTCT CCTCCTCTAA AGCTGTGGAG 
AGGGAGAATG TCGAAATGGG AGCAGATCCA CTCTCACTCT TTGCTTCAGA AACTGAAGAC 
ACAACCTCCA TTCAGAGCCA GGAGTCCACA CGGACCTTTC CACCTCTGGT GTCAAGGAAT 
CTTGCAGATG AGATTGAGAT GTACATGAGC CTCCAAAGCC CCCTGACTGC TAGATCTCCT 
AGCATGGAGT TGCAACAGGG TTCAAACCCT GAATCCATAA ACTGTCCAGA CTCTCCAGCA 
GTTAAGCAGT CTTTAGAAAG AAGGTCAAGC CTTCCGATTG GCCCAGTCAA AGTGGCCAGT 
TCAACGGAGC ATCCAAAACC TAGTCCTGCT GTCACTCGCT CAAAGACATT TGCTGTCAAA 
TCCCCCAAGA CCCCTGGCTC TGCAGGCCAG AGGTCGTCAT CGCTGACTGC ACTGGTAAAA 
TCGTCACAGA ACAGCTCACT AGGCTCTGTC ATCAACTCCA TCTCAGGCAT CAAGATGGAC 
TCTCTTCTGG TGGGCCCCAA AGTAGATATG CTTAAATCAG GCATGAAGCA GGCAGCCAAT 
GTGGCCAGTA AGATGTGGGA TGCAGTGGCT TCAGCCTACT CCTACTCAGA TGATGAGGAG 
GATAACTTAA AGCCCCAGTA CAGTTTCCCA GCTAGGCTAG AAGAACAGTT GCTATCTGGA 
GAGGGAGATA CGGAGAACAC TTTACCCAGA GGACTGATGC CTAGTCTGGG CTCAAACGGG 
CTCGCACACA GCAATACCAG CCTGGGTAGC AGCAGTGGAA GCAGCGATAC CGGCCAGGCA 
CACCTGTCAA CTCCTTTGAC TCCAGGTCGC TCAATGAGAG GAGCAGACTC CGAGAGGTCT 
GAACATAGTT CCTCTCATCA TGCCAGCACC TCCAGCATTT ACCAAAACTG TGCTTTAGAG 
GTTCTCATGT CCAGCTGCTC TCAGTGTCGC TCTTGTGAGG CACTAGTGTA TGATGAGGAG 
ATCATGGCGG GCTGGACAGC AGATGACTCC AATCTAAACT GCACCTGTCC CTTCTGTCAA 
GTCACTTTCC TTCCTTTGCT CCATGTTGAG TTCCAGGACC TGCGTGCTAC TCCAGGCTTC 
TACTTGAAGA CGAGTACATC AGGAGACAGT ATCCACAGCT CCACCACACA ACCAACCACT 
TCTAGCTCAT CAGAAGTGAA AGTGAGCTCT CCTAGTGACC CTTCAGACCT CATGACTTTC 
GCAGAATCTT CTGAGGACAG CAAGAGTCCT GTTGAGATGC CTGTTGCTCA AACTGGAACC 
CCAAAGATCC TTGTACCTGA GCCTGTACAT TCAGACCCCC TTGGTCTCAT GGAGAGACAA 
GCTGAGAAAC GGAACACATT AACACGGAGC AACAGTATGG GAGGACCACT GCAGAATTTG 
GACTTGATTC AAAGACCCAA TCATGGCATA TCCACTATTA GCCTGCCCAA CAGCTTACAA 
GAAGTGTCGG ACACGTTGGG TCAAAAGCGA CCCAACCCAA AACCAGTTTC TGTGCCGTAT 
CTCAGTCCAC TGGTTCTGCG CAAAGAATTG GAGTCGCTTT TAGAAAATGA AGGGGATCAG 
GTAATCTATA CACATAAGTT TCTTAGTCAG CACCCCATCA TTTTCTGGAA CCTGGTGTGG 
TATTTTCGTC GTTTAGACCT GCCCAGCATT CTGCCTGGAC TCATTCTCAC TTCTGAACAC 
TGCAACAATG GAGTAAAGCT GCCGCAGACA TCTTTGTCTC AGGACAGTAA GCAGGTGTAC 
GTGCAGCTGT TGTGGGATAA TGTCAACTTA CATCAGGAGC CCACCGAGCC CCTCTATCAG 
CTCTGGAGGA CCTTCTTGCA AAGGAAGGGA ACTCTAGCCC CAACTGACCA CCAGGAGACT 
CGGTCTTTGC TAAACAGCAT TGTACGCAGC ATTCAGATGA ACGATGTTTA CGGCCCCATC 
AATATGCTCA TCCGGGAGAT CAAGAAACAT CCAGAAGTTA AACGCCAGAG GAGTGTCTAC 
AGGGAAATTA TGTTTCTGTC ACTTGTTGCC TTAGGAAGGG AGAACATTGA TATTGAGGCC 
TTTAATCGTG AATACCGTCT TGCTTACAGC CAGCTCAGCG CAGAACAGCT TAAAGTCCTG 
TCCCCTATTG ACAGACCACC TAGCAACGGT GTGCAGTGGT GCCTCAATTC CTTTGGAGCC 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166610.1
CDS	484..6315
Translation	MIEDKGHRVTDYFVVAGLTDKSTPLEQDISEAKSSGPKAPITDLAVINKSSGETVPEGFTCIESTYSGQPANLNHGSLKSPELFLCYKRGRGKPPLIDIGVLYEGKERLIQGCEVIEATPFGRCANVNNSSATSQRIFITYRRAPPVQPQNSLAVTDICVIITNKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVSSSNSIAYKAGLIFRYPEEDYESFPLSESVPLFCLPMGAKIECWAPNTRYPLPVFSTFVLTNSNGEKVYGAGIQFYEPYPVDGLSEKQKIQLGLVSAVDKKVLPNRTVNTNKCICLLSRWPFFEAFRKFLMFLYKLSANGVSPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLILSQPVCTPLPLSGADYSTLLMNLGPENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKRHSNWKNLPKKPSKSLISFLGSLHHQLATVRRTATEGLAVEMTPIEADFTWHKKKTELEMEIQEVFLRFMASILKGYRTYLKPITQAPSEKATAADSLYDLQGFLKSRDRAHQKFYSQLTKTQIFIRFVEECTFVSDKDTGLAFFDDCIRKLFPSDKGTDKGTKVEGESSEDTRLMELDESQKSEHTVFVMPPEPPLEDGPDPPPKYSYKNFPSLRLELFDRPLELRPALSTRAAGSSVSSSPALLAKRTKQEIKMAYKLAKRFYSNPPQWAKCLCSHCYSLWFICLPARMRTAQSKPRAMQQAFNVLLKMRSSEVEVLDEVCYRVVMQLCGLWEMPVMAVRVLMEMKKAGVEPNAITYGYYNKAVLESPWPSRNRSGLFMWTKMRNLVRGVVQFKRALRGTTLQIGSSLKSTDLSASTAVGADGDRLSHGSVDGIVEEHILFSHSILVEDVIDSHYSTAAQSDQGYGSKDELPQEHTEEPVTSPPLSTNHTCSSKETNTKNGQDIASSVDSVVAVTEPPSPAEIAPCVASIVKPSTDIPVHFDSTAGRGETAGVCTGKLFMSRKKSEDVALSVEDATVIPDSQLPQSGQQQRVKAFMERSCSFSAESKAGMLQKKSSLELSADHMGADAKILAAVFSGSQTPPPFASIAPFKEFKVEATLSPALQEEEQEEKQETKHESFTSAAEGVEKSIKRDNAAAESNSSERVGRTTGSAVSSSKAVERENVEMGADPLSLFASETEDTTSIQSQESTRTFPPLVSRNLADEIEMYMSLQSPLTARSPSMELQQGSNPESINCPDSPAVKQSLERRSSLPIGPVKVASSTEHPKPSPAVTRSKTFAVKSPKTPGSAGQRSSSLTALVKSSQNSSLGSVINSISGIKMDSLLVGPKVDMLKSGMKQAANVASKMWDAVASAYSYSDDEEDNLKPQYSFPARLEEQLLSGEGDTENTLPRGLMPSLGSNGLAHSNTSLGSSSGSSDTGQAHLSTPLTPGRSMRGADSERSEHSSSHHASTSSIYQNCALEVLMSSCSQCRSCEALVYDEEIMAGWTADDSNLNCTCPFCQVTFLPLLHVEFQDLRATPGFYLKTSTSGDSIHSSTTQPTTSSSSEVKVSSPSDPSDLMTFAESSEDSKSPVEMPVAQTGTPKILVPEPVHSDPLGLMERQAEKRNTLTRSNSMGGPLQNLDLIQRPNHGISTISLPNSLQEVSDTLGQKRPNPKPVSVPYLSPLVLRKELESLLENEGDQVIYTHKFLSQHPIIFWNLVWYFRRLDLPSILPGLILTSEHCNNGVKLPQTSLSQDSKQVYVQLLWDNVNLHQEPTEPLYQLWRTFLQRKGTLAPTDHQETRSLLNSIVRSIQMNDVYGPINMLIREIKKHPEVKRQRSVYREIMFLSLVALGRENIDIEAFNREYRLAYSQLSAEQLKVLSPIDRPPSNGVQWCLNSFGAPVI

Target ORF information:

RefSeq Version	XM_005166553.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166553.3

ORF Insert Sequence:

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GCAGGTGTTT GCACTGGTAA GCTCTTCATG 
TCCCGTAAAA AGAGTGAAGA TGTGGCCTTG TCTGTGGAGG ATGCTACTGT AATCCCAGAT 
TCACAGCTTC CGCAGTCTGG ACAGCAGCAA AGGGTAAAGG CCTTCATGGA GCGCAGCTGC 
AGTTTTAGTG CAGAGAGCAA AGCTGGCATG CTACAGAAAA AAAGCAGTCT GGAGCTCAGT 
GCTGATCACA TGGGCGCAGA TGCAAAAATT CTTGCAGCAG TGTTCTCCGG CAGCCAGACT 
CCTCCTCCTT TTGCAAGCAT TGCTCCGTTT AAAGAGTTCA AGGTGGAGGC TACACTAAGC 
CCTGCCCTCC AGGAGGAAGA ACAAGAGGAA AAGCAGGAGA CCAAACATGA ATCCTTCACT 
TCTGCAGCAG AGGGAGTGGA GAAAAGTATA AAGCGGGATA ATGCAGCAGC AGAATCCAAT 
AGCAGTGAAC GAGTTGGGCG AACCACAGGG AGTGCCGTCT CCTCCTCTAA AGCTGTGGAG 
AGGGAGAATG TCGAAATGGG AGCAGATCCA CTCTCACTCT TTGCTTCAGA AACTGAAGAC 
ACAACCTCCA TTCAGAGCCA GGAGTCCACA CGGACCTTTC CACCTCTGGT GTCAAGGAAT 
CTTGCAGATG AGATTGAGAT GTACATGAGC CTCCAAAGCC CCCTGACTGC TAGATCTCCT 
AGCATGGAGT TGCAACAGGG TTCAAACCCT GAATCCATAA ACTGTCCAGA CTCTCCAGCA 
GTTAAGCAGT CTTTAGAAAG AAGGTCAAGC CTTCCGATTG GCCCAGTCAA AGTGGCCAGT 
TCAACGGAGC ATCCAAAACC TAGTCCTGCT GTCACTCGCT CAAAGACATT TGCTGTCAAA 
TCCCCCAAGA CCCCTGGCTC TGCAGGCCAG AGGTCGTCAT CGCTGACTGC ACTGGTAAAA 
TCGTCACAGA ACAGCTCACT AGGCTCTGTC ATCAACTCCA TCTCAGGCAT CAAGATGGAC 
TCTCTTCTGG TGGGCCCCAA AGTAGATATG CTTAAATCAG GCATGAAGCA GGCAGCCAAT 
GTGGCCAGTA AGATGTGGGA TGCAGTGGCT TCAGCCTACT CCTACTCAGA TGATGAGGAG 
GATAACTTAA AGCCCCAGTA CAGTTTCCCA GCTAGGCTAG AAGAACAGTT GCTATCTGGA 
GAGGGAGATA CGGAGAACAC TTTACCCAGA GGACTGATGC CTAGTCTGGG CTCAAACGGG 
CTCGCACACA GCAATACCAG CCTGGGTAGC AGCAGTGGAA GCAGCGATAC CGGCCAGGCA 
CACCTGTCAA CTCCTTTGAC TCCAGGTCGC TCAATGAGAG GAGCAGACTC CGAGAGGTCT 
GAACATAGTT CCTCTCATCA TGCCAGCACC TCCAGCATTT ACCAAAACTG TGCTTTAGAG 
GTTCTCATGT CCAGCTGCTC TCAGTGTCGC TCTTGTGAGG CACTAGTGTA TGATGAGGAG 
ATCATGGCGG GCTGGACAGC AGATGACTCC AATCTAAACT GCACCTGTCC CTTCTGTCAA 
GTCACTTTCC TTCCTTTGCT CCATGTTGAG TTCCAGGACC TGCGTGCTAC TCCAGGCTTC 
TACTTGAAGA CGAGTACATC AGGAGACAGT ATCCACAGCT CCACCACACA ACCAACCACT 
TCTAGCTCAT CAGAAGTGAA AGTGAGCTCT CCTAGTGACC CTTCAGACCT CATGACTTTC 
GCAGAATCTT CTGAGGACAG CAAGAGTCCT GTTGAGATGC CTGTTGCTCA AACTGGAACC 
CCAAAGATCC TTGTACCTGA GCCTGTACAT TCAGACCCCC TTGGTCTCAT GGAGAGACAA 
GCTGAGAAAC GGAACACATT AACACGGAGC AACAGTATGG GAGGACCACT GCAGAATTTG 
GACTTGATTC AAAGACCCAA TCATGGCATA TCCACTATTA GCCTGCCCAA CAGCTTACAA 
GAAGTGTCGG ACACGTTGGG TCAAAAGCGA CCCAACCCAA AACCAGTTTC TGTGCCGTAT 
CTCAGTCCAC TGGTTCTGCG CAAAGAATTG GAGTCGCTTT TAGAAAATGA AGGGGATCAG 
GTAATCTATA CACATAAGTT TCTTAGTCAG CACCCCATCA TTTTCTGGAA CCTGGTGTGG 
TATTTTCGTC GTTTAGACCT GCCCAGCATT CTGCCTGGAC TCATTCTCAC TTCTGAACAC 
TGCAACAATG GAGTAAAGCT GCCGCAGACA TCTTTGTCTC AGGACAGTAA GCAGGTGTAC 
GTGCAGCTGT TGTGGGATAA TGTCAACTTA CATCAGGAGC CCACCGAGCC CCTCTATCAG 
CTCTGGAGGA CCTTCTTGCA AAGGAAGGGA ACTCTAGCCC CAACTGACCA CCAGGAGACT 
CGGTCTTTGC TAAACAGCAT TGTACGCAGC ATTCAGATGA ACGATGTTTA CGGCCCCATC 
AATATGCTCA TCCGGGAGAT CAAGAAACAT CCAGAAGTTA AACGCCAGAG GAGTGTCTAC 
AGGGAAATTA TGTTTCTGTC ACTTGTTGCC TTAGGAAGGG AGAACATTGA TATTGAGGCC 
TTTAATCGTG AATACCGTCT TGCTTACAGC CAGCTCAGCG CAGAACAGCT TAAAGTCCTG 
TCCCCTATTG ACAGACCACC TAGCAACGGT GTGCAGTGGT GCCTCAATTC CTTTGGAGCC 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4C ( XM_005166553.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005166610.1 Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

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CloneID	ODa13591
Clone ID Related Accession (Same CDS sequence)	XM_005166553.3 , XM_005166553.4
Accession Version	XM_005166553.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 4C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166553.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GCAGGTGTTT GCACTGGTAA GCTCTTCATG 
TCCCGTAAAA AGAGTGAAGA TGTGGCCTTG TCTGTGGAGG ATGCTACTGT AATCCCAGAT 
TCACAGCTTC CGCAGTCTGG ACAGCAGCAA AGGGTAAAGG CCTTCATGGA GCGCAGCTGC 
AGTTTTAGTG CAGAGAGCAA AGCTGGCATG CTACAGAAAA AAAGCAGTCT GGAGCTCAGT 
GCTGATCACA TGGGCGCAGA TGCAAAAATT CTTGCAGCAG TGTTCTCCGG CAGCCAGACT 
CCTCCTCCTT TTGCAAGCAT TGCTCCGTTT AAAGAGTTCA AGGTGGAGGC TACACTAAGC 
CCTGCCCTCC AGGAGGAAGA ACAAGAGGAA AAGCAGGAGA CCAAACATGA ATCCTTCACT 
TCTGCAGCAG AGGGAGTGGA GAAAAGTATA AAGCGGGATA ATGCAGCAGC AGAATCCAAT 
AGCAGTGAAC GAGTTGGGCG AACCACAGGG AGTGCCGTCT CCTCCTCTAA AGCTGTGGAG 
AGGGAGAATG TCGAAATGGG AGCAGATCCA CTCTCACTCT TTGCTTCAGA AACTGAAGAC 
ACAACCTCCA TTCAGAGCCA GGAGTCCACA CGGACCTTTC CACCTCTGGT GTCAAGGAAT 
CTTGCAGATG AGATTGAGAT GTACATGAGC CTCCAAAGCC CCCTGACTGC TAGATCTCCT 
AGCATGGAGT TGCAACAGGG TTCAAACCCT GAATCCATAA ACTGTCCAGA CTCTCCAGCA 
GTTAAGCAGT CTTTAGAAAG AAGGTCAAGC CTTCCGATTG GCCCAGTCAA AGTGGCCAGT 
TCAACGGAGC ATCCAAAACC TAGTCCTGCT GTCACTCGCT CAAAGACATT TGCTGTCAAA 
TCCCCCAAGA CCCCTGGCTC TGCAGGCCAG AGGTCGTCAT CGCTGACTGC ACTGGTAAAA 
TCGTCACAGA ACAGCTCACT AGGCTCTGTC ATCAACTCCA TCTCAGGCAT CAAGATGGAC 
TCTCTTCTGG TGGGCCCCAA AGTAGATATG CTTAAATCAG GCATGAAGCA GGCAGCCAAT 
GTGGCCAGTA AGATGTGGGA TGCAGTGGCT TCAGCCTACT CCTACTCAGA TGATGAGGAG 
GATAACTTAA AGCCCCAGTA CAGTTTCCCA GCTAGGCTAG AAGAACAGTT GCTATCTGGA 
GAGGGAGATA CGGAGAACAC TTTACCCAGA GGACTGATGC CTAGTCTGGG CTCAAACGGG 
CTCGCACACA GCAATACCAG CCTGGGTAGC AGCAGTGGAA GCAGCGATAC CGGCCAGGCA 
CACCTGTCAA CTCCTTTGAC TCCAGGTCGC TCAATGAGAG GAGCAGACTC CGAGAGGTCT 
GAACATAGTT CCTCTCATCA TGCCAGCACC TCCAGCATTT ACCAAAACTG TGCTTTAGAG 
GTTCTCATGT CCAGCTGCTC TCAGTGTCGC TCTTGTGAGG CACTAGTGTA TGATGAGGAG 
ATCATGGCGG GCTGGACAGC AGATGACTCC AATCTAAACT GCACCTGTCC CTTCTGTCAA 
GTCACTTTCC TTCCTTTGCT CCATGTTGAG TTCCAGGACC TGCGTGCTAC TCCAGGCTTC 
TACTTGAAGA CGAGTACATC AGGAGACAGT ATCCACAGCT CCACCACACA ACCAACCACT 
TCTAGCTCAT CAGAAGTGAA AGTGAGCTCT CCTAGTGACC CTTCAGACCT CATGACTTTC 
GCAGAATCTT CTGAGGACAG CAAGAGTCCT GTTGAGATGC CTGTTGCTCA AACTGGAACC 
CCAAAGATCC TTGTACCTGA GCCTGTACAT TCAGACCCCC TTGGTCTCAT GGAGAGACAA 
GCTGAGAAAC GGAACACATT AACACGGAGC AACAGTATGG GAGGACCACT GCAGAATTTG 
GACTTGATTC AAAGACCCAA TCATGGCATA TCCACTATTA GCCTGCCCAA CAGCTTACAA 
GAAGTGTCGG ACACGTTGGG TCAAAAGCGA CCCAACCCAA AACCAGTTTC TGTGCCGTAT 
CTCAGTCCAC TGGTTCTGCG CAAAGAATTG GAGTCGCTTT TAGAAAATGA AGGGGATCAG 
GTAATCTATA CACATAAGTT TCTTAGTCAG CACCCCATCA TTTTCTGGAA CCTGGTGTGG 
TATTTTCGTC GTTTAGACCT GCCCAGCATT CTGCCTGGAC TCATTCTCAC TTCTGAACAC 
TGCAACAATG GAGTAAAGCT GCCGCAGACA TCTTTGTCTC AGGACAGTAA GCAGGTGTAC 
GTGCAGCTGT TGTGGGATAA TGTCAACTTA CATCAGGAGC CCACCGAGCC CCTCTATCAG 
CTCTGGAGGA CCTTCTTGCA AAGGAAGGGA ACTCTAGCCC CAACTGACCA CCAGGAGACT 
CGGTCTTTGC TAAACAGCAT TGTACGCAGC ATTCAGATGA ACGATGTTTA CGGCCCCATC 
AATATGCTCA TCCGGGAGAT CAAGAAACAT CCAGAAGTTA AACGCCAGAG GAGTGTCTAC 
AGGGAAATTA TGTTTCTGTC ACTTGTTGCC TTAGGAAGGG AGAACATTGA TATTGAGGCC 
TTTAATCGTG AATACCGTCT TGCTTACAGC CAGCTCAGCG CAGAACAGCT TAAAGTCCTG 
TCCCCTATTG ACAGACCACC TAGCAACGGT GTGCAGTGGT GCCTCAATTC CTTTGGAGCC 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166610.1
CDS	257..6088
Translation	MIEDKGHRVTDYFVVAGLTDKSTPLEQDISEAKSSGPKAPITDLAVINKSSGETVPEGFTCIESTYSGQPANLNHGSLKSPELFLCYKRGRGKPPLIDIGVLYEGKERLIQGCEVIEATPFGRCANVNNSSATSQRIFITYRRAPPVQPQNSLAVTDICVIITNKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVSSSNSIAYKAGLIFRYPEEDYESFPLSESVPLFCLPMGAKIECWAPNTRYPLPVFSTFVLTNSNGEKVYGAGIQFYEPYPVDGLSEKQKIQLGLVSAVDKKVLPNRTVNTNKCICLLSRWPFFEAFRKFLMFLYKLSANGVSPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLILSQPVCTPLPLSGADYSTLLMNLGPENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKRHSNWKNLPKKPSKSLISFLGSLHHQLATVRRTATEGLAVEMTPIEADFTWHKKKTELEMEIQEVFLRFMASILKGYRTYLKPITQAPSEKATAADSLYDLQGFLKSRDRAHQKFYSQLTKTQIFIRFVEECTFVSDKDTGLAFFDDCIRKLFPSDKGTDKGTKVEGESSEDTRLMELDESQKSEHTVFVMPPEPPLEDGPDPPPKYSYKNFPSLRLELFDRPLELRPALSTRAAGSSVSSSPALLAKRTKQEIKMAYKLAKRFYSNPPQWAKCLCSHCYSLWFICLPARMRTAQSKPRAMQQAFNVLLKMRSSEVEVLDEVCYRVVMQLCGLWEMPVMAVRVLMEMKKAGVEPNAITYGYYNKAVLESPWPSRNRSGLFMWTKMRNLVRGVVQFKRALRGTTLQIGSSLKSTDLSASTAVGADGDRLSHGSVDGIVEEHILFSHSILVEDVIDSHYSTAAQSDQGYGSKDELPQEHTEEPVTSPPLSTNHTCSSKETNTKNGQDIASSVDSVVAVTEPPSPAEIAPCVASIVKPSTDIPVHFDSTAGRGETAGVCTGKLFMSRKKSEDVALSVEDATVIPDSQLPQSGQQQRVKAFMERSCSFSAESKAGMLQKKSSLELSADHMGADAKILAAVFSGSQTPPPFASIAPFKEFKVEATLSPALQEEEQEEKQETKHESFTSAAEGVEKSIKRDNAAAESNSSERVGRTTGSAVSSSKAVERENVEMGADPLSLFASETEDTTSIQSQESTRTFPPLVSRNLADEIEMYMSLQSPLTARSPSMELQQGSNPESINCPDSPAVKQSLERRSSLPIGPVKVASSTEHPKPSPAVTRSKTFAVKSPKTPGSAGQRSSSLTALVKSSQNSSLGSVINSISGIKMDSLLVGPKVDMLKSGMKQAANVASKMWDAVASAYSYSDDEEDNLKPQYSFPARLEEQLLSGEGDTENTLPRGLMPSLGSNGLAHSNTSLGSSSGSSDTGQAHLSTPLTPGRSMRGADSERSEHSSSHHASTSSIYQNCALEVLMSSCSQCRSCEALVYDEEIMAGWTADDSNLNCTCPFCQVTFLPLLHVEFQDLRATPGFYLKTSTSGDSIHSSTTQPTTSSSSEVKVSSPSDPSDLMTFAESSEDSKSPVEMPVAQTGTPKILVPEPVHSDPLGLMERQAEKRNTLTRSNSMGGPLQNLDLIQRPNHGISTISLPNSLQEVSDTLGQKRPNPKPVSVPYLSPLVLRKELESLLENEGDQVIYTHKFLSQHPIIFWNLVWYFRRLDLPSILPGLILTSEHCNNGVKLPQTSLSQDSKQVYVQLLWDNVNLHQEPTEPLYQLWRTFLQRKGTLAPTDHQETRSLLNSIVRSIQMNDVYGPINMLIREIKKHPEVKRQRSVYREIMFLSLVALGRENIDIEAFNREYRLAYSQLSAEQLKVLSPIDRPPSNGVQWCLNSFGAPVI

Target ORF information:

RefSeq Version	XM_005166553.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166553.4

ORF Insert Sequence:

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GCAGGTGTTT GCACTGGTAA GCTCTTCATG 
TCCCGTAAAA AGAGTGAAGA TGTGGCCTTG TCTGTGGAGG ATGCTACTGT AATCCCAGAT 
TCACAGCTTC CGCAGTCTGG ACAGCAGCAA AGGGTAAAGG CCTTCATGGA GCGCAGCTGC 
AGTTTTAGTG CAGAGAGCAA AGCTGGCATG CTACAGAAAA AAAGCAGTCT GGAGCTCAGT 
GCTGATCACA TGGGCGCAGA TGCAAAAATT CTTGCAGCAG TGTTCTCCGG CAGCCAGACT 
CCTCCTCCTT TTGCAAGCAT TGCTCCGTTT AAAGAGTTCA AGGTGGAGGC TACACTAAGC 
CCTGCCCTCC AGGAGGAAGA ACAAGAGGAA AAGCAGGAGA CCAAACATGA ATCCTTCACT 
TCTGCAGCAG AGGGAGTGGA GAAAAGTATA AAGCGGGATA ATGCAGCAGC AGAATCCAAT 
AGCAGTGAAC GAGTTGGGCG AACCACAGGG AGTGCCGTCT CCTCCTCTAA AGCTGTGGAG 
AGGGAGAATG TCGAAATGGG AGCAGATCCA CTCTCACTCT TTGCTTCAGA AACTGAAGAC 
ACAACCTCCA TTCAGAGCCA GGAGTCCACA CGGACCTTTC CACCTCTGGT GTCAAGGAAT 
CTTGCAGATG AGATTGAGAT GTACATGAGC CTCCAAAGCC CCCTGACTGC TAGATCTCCT 
AGCATGGAGT TGCAACAGGG TTCAAACCCT GAATCCATAA ACTGTCCAGA CTCTCCAGCA 
GTTAAGCAGT CTTTAGAAAG AAGGTCAAGC CTTCCGATTG GCCCAGTCAA AGTGGCCAGT 
TCAACGGAGC ATCCAAAACC TAGTCCTGCT GTCACTCGCT CAAAGACATT TGCTGTCAAA 
TCCCCCAAGA CCCCTGGCTC TGCAGGCCAG AGGTCGTCAT CGCTGACTGC ACTGGTAAAA 
TCGTCACAGA ACAGCTCACT AGGCTCTGTC ATCAACTCCA TCTCAGGCAT CAAGATGGAC 
TCTCTTCTGG TGGGCCCCAA AGTAGATATG CTTAAATCAG GCATGAAGCA GGCAGCCAAT 
GTGGCCAGTA AGATGTGGGA TGCAGTGGCT TCAGCCTACT CCTACTCAGA TGATGAGGAG 
GATAACTTAA AGCCCCAGTA CAGTTTCCCA GCTAGGCTAG AAGAACAGTT GCTATCTGGA 
GAGGGAGATA CGGAGAACAC TTTACCCAGA GGACTGATGC CTAGTCTGGG CTCAAACGGG 
CTCGCACACA GCAATACCAG CCTGGGTAGC AGCAGTGGAA GCAGCGATAC CGGCCAGGCA 
CACCTGTCAA CTCCTTTGAC TCCAGGTCGC TCAATGAGAG GAGCAGACTC CGAGAGGTCT 
GAACATAGTT CCTCTCATCA TGCCAGCACC TCCAGCATTT ACCAAAACTG TGCTTTAGAG 
GTTCTCATGT CCAGCTGCTC TCAGTGTCGC TCTTGTGAGG CACTAGTGTA TGATGAGGAG 
ATCATGGCGG GCTGGACAGC AGATGACTCC AATCTAAACT GCACCTGTCC CTTCTGTCAA 
GTCACTTTCC TTCCTTTGCT CCATGTTGAG TTCCAGGACC TGCGTGCTAC TCCAGGCTTC 
TACTTGAAGA CGAGTACATC AGGAGACAGT ATCCACAGCT CCACCACACA ACCAACCACT 
TCTAGCTCAT CAGAAGTGAA AGTGAGCTCT CCTAGTGACC CTTCAGACCT CATGACTTTC 
GCAGAATCTT CTGAGGACAG CAAGAGTCCT GTTGAGATGC CTGTTGCTCA AACTGGAACC 
CCAAAGATCC TTGTACCTGA GCCTGTACAT TCAGACCCCC TTGGTCTCAT GGAGAGACAA 
GCTGAGAAAC GGAACACATT AACACGGAGC AACAGTATGG GAGGACCACT GCAGAATTTG 
GACTTGATTC AAAGACCCAA TCATGGCATA TCCACTATTA GCCTGCCCAA CAGCTTACAA 
GAAGTGTCGG ACACGTTGGG TCAAAAGCGA CCCAACCCAA AACCAGTTTC TGTGCCGTAT 
CTCAGTCCAC TGGTTCTGCG CAAAGAATTG GAGTCGCTTT TAGAAAATGA AGGGGATCAG 
GTAATCTATA CACATAAGTT TCTTAGTCAG CACCCCATCA TTTTCTGGAA CCTGGTGTGG 
TATTTTCGTC GTTTAGACCT GCCCAGCATT CTGCCTGGAC TCATTCTCAC TTCTGAACAC 
TGCAACAATG GAGTAAAGCT GCCGCAGACA TCTTTGTCTC AGGACAGTAA GCAGGTGTAC 
GTGCAGCTGT TGTGGGATAA TGTCAACTTA CATCAGGAGC CCACCGAGCC CCTCTATCAG 
CTCTGGAGGA CCTTCTTGCA AAGGAAGGGA ACTCTAGCCC CAACTGACCA CCAGGAGACT 
CGGTCTTTGC TAAACAGCAT TGTACGCAGC ATTCAGATGA ACGATGTTTA CGGCCCCATC 
AATATGCTCA TCCGGGAGAT CAAGAAACAT CCAGAAGTTA AACGCCAGAG GAGTGTCTAC 
AGGGAAATTA TGTTTCTGTC ACTTGTTGCC TTAGGAAGGG AGAACATTGA TATTGAGGCC 
TTTAATCGTG AATACCGTCT TGCTTACAGC CAGCTCAGCG CAGAACAGCT TAAAGTCCTG 
TCCCCTATTG ACAGACCACC TAGCAACGGT GTGCAGTGGT GCCTCAATTC CTTTGGAGCC 
CCAGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4C ( XM_001919955.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001919990.2 Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

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CloneID	ODa13592
Clone ID Related Accession (Same CDS sequence)	XM_001919955.7 , XM_001919955.6
Accession Version	XM_001919955.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 4C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919955.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GGTGTTTGCA CTGGTAAGCT CTTCATGTCC 
CGTAAAAAGA GTGAAGATGT GGCCTTGTCT GTGGAGGATG CTACTGTAAT CCCAGATTCA 
CAGCTTCCGC AGTCTGGACA GCAGCAAAGG GTAAAGGCCT TCATGGAGCG CAGCTGCAGT 
TTTAGTGCAG AGAGCAAAGC TGGCATGCTA CAGAAAAAAA GCAGTCTGGA GCTCAGTGCT 
GATCACATGG GCGCAGATGC AAAAATTCTT GCAGCAGTGT TCTCCGGCAG CCAGACTCCT 
CCTCCTTTTG CAAGCATTGC TCCGTTTAAA GAGTTCAAGG TGGAGGCTAC ACTAAGCCCT 
GCCCTCCAGG AGGAAGAACA AGAGGAAAAG CAGGAGACCA AACATGAATC CTTCACTTCT 
GCAGCAGAGG GAGTGGAGAA AAGTATAAAG CGGGATAATG CAGCAGCAGA ATCCAATAGC 
AGTGAACGAG TTGGGCGAAC CACAGGGAGT GCCGTCTCCT CCTCTAAAGC TGTGGAGAGG 
GAGAATGTCG AAATGGGAGC AGATCCACTC TCACTCTTTG CTTCAGAAAC TGAAGACACA 
ACCTCCATTC AGAGCCAGGA GTCCACACGG ACCTTTCCAC CTCTGGTGTC AAGGAATCTT 
GCAGATGAGA TTGAGATGTA CATGAGCCTC CAAAGCCCCC TGACTGCTAG ATCTCCTAGC 
ATGGAGTTGC AACAGGGTTC AAACCCTGAA TCCATAAACT GTCCAGACTC TCCAGCAGTT 
AAGCAGTCTT TAGAAAGAAG GTCAAGCCTT CCGATTGGCC CAGTCAAAGT GGCCAGTTCA 
ACGGAGCATC CAAAACCTAG TCCTGCTGTC ACTCGCTCAA AGACATTTGC TGTCAAATCC 
CCCAAGACCC CTGGCTCTGC AGGCCAGAGG TCGTCATCGC TGACTGCACT GGTAAAATCG 
TCACAGAACA GCTCACTAGG CTCTGTCATC AACTCCATCT CAGGCATCAA GATGGACTCT 
CTTCTGGTGG GCCCCAAAGT AGATATGCTT AAATCAGGCA TGAAGCAGGC AGCCAATGTG 
GCCAGTAAGA TGTGGGATGC AGTGGCTTCA GCCTACTCCT ACTCAGATGA TGAGGAGGAT 
AACTTAAAGC CCCAGTACAG TTTCCCAGCT AGGCTAGAAG AACAGTTGCT ATCTGGAGAG 
GGAGATACGG AGAACACTTT ACCCAGAGGA CTGATGCCTA GTCTGGGCTC AAACGGGCTC 
GCACACAGCA ATACCAGCCT GGGTAGCAGC AGTGGAAGCA GCGATACCGG CCAGGCACAC 
CTGTCAACTC CTTTGACTCC AGGTCGCTCA ATGAGAGGAG CAGACTCCGA GAGGTCTGAA 
CATAGTTCCT CTCATCATGC CAGCACCTCC AGCATTTACC AAAACTGTGC TTTAGAGGTT 
CTCATGTCCA GCTGCTCTCA GTGTCGCTCT TGTGAGGCAC TAGTGTATGA TGAGGAGATC 
ATGGCGGGCT GGACAGCAGA TGACTCCAAT CTAAACTGCA CCTGTCCCTT CTGTCAAGTC 
ACTTTCCTTC CTTTGCTCCA TGTTGAGTTC CAGGACCTGC GTGCTACTCC AGGCTTCTAC 
TTGAAGACGA GTACATCAGG AGACAGTATC CACAGCTCCA CCACACAACC AACCACTTCT 
AGCTCATCAG AAGTGAAAGT GAGCTCTCCT AGTGACCCTT CAGACCTCAT GACTTTCGCA 
GAATCTTCTG AGGACAGCAA GAGTCCTGTT GAGATGCCTG TTGCTCAAAC TGGAACCCCA 
AAGATCCTTG TACCTGAGCC TGTACATTCA GACCCCCTTG GTCTCATGGA GAGACAAGCT 
GAGAAACGGA ACACATTAAC ACGGAGCAAC AGTATGGGAG GACCACTGCA GAATTTGGAC 
TTGATTCAAA GACCCAATCA TGGCATATCC ACTATTAGCC TGCCCAACAG CTTACAAGAA 
GTGTCGGACA CGTTGGGTCA AAAGCGACCC AACCCAAAAC CAGTTTCTGT GCCGTATCTC 
AGTCCACTGG TTCTGCGCAA AGAATTGGAG TCGCTTTTAG AAAATGAAGG GGATCAGGTA 
ATCTATACAC ATAAGTTTCT TAGTCAGCAC CCCATCATTT TCTGGAACCT GGTGTGGTAT 
TTTCGTCGTT TAGACCTGCC CAGCATTCTG CCTGGACTCA TTCTCACTTC TGAACACTGC 
AACAATGGAG TAAAGCTGCC GCAGACATCT TTGTCTCAGG ACAGTAAGCA GGTGTACGTG 
CAGCTGTTGT GGGATAATGT CAACTTACAT CAGGAGCCCA CCGAGCCCCT CTATCAGCTC 
TGGAGGACCT TCTTGCAAAG GAAGGGAACT CTAGCCCCAA CTGACCACCA GGAGACTCGG 
TCTTTGCTAA ACAGCATTGT ACGCAGCATT CAGATGAACG ATGTTTACGG CCCCATCAAT 
ATGCTCATCC GGGAGATCAA GAAACATCCA GAAGTTAAAC GCCAGAGGAG TGTCTACAGG 
GAAATTATGT TTCTGTCACT TGTTGCCTTA GGAAGGGAGA ACATTGATAT TGAGGCCTTT 
AATCGTGAAT ACCGTCTTGC TTACAGCCAG CTCAGCGCAG AACAGCTTAA AGTCCTGTCC 
CCTATTGACA GACCACCTAG CAACGGTGTG CAGTGGTGCC TCAATTCCTT TGGAGCCCCA 
GTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919990.2
CDS	256..6084
Translation	MIEDKGHRVTDYFVVAGLTDKSTPLEQDISEAKSSGPKAPITDLAVINKSSGETVPEGFTCIESTYSGQPANLNHGSLKSPELFLCYKRGRGKPPLIDIGVLYEGKERLIQGCEVIEATPFGRCANVNNSSATSQRIFITYRRAPPVQPQNSLAVTDICVIITNKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVSSSNSIAYKAGLIFRYPEEDYESFPLSESVPLFCLPMGAKIECWAPNTRYPLPVFSTFVLTNSNGEKVYGAGIQFYEPYPVDGLSEKQKIQLGLVSAVDKKVLPNRTVNTNKCICLLSRWPFFEAFRKFLMFLYKLSANGVSPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLILSQPVCTPLPLSGADYSTLLMNLGPENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKRHSNWKNLPKKPSKSLISFLGSLHHQLATVRRTATEGLAVEMTPIEADFTWHKKKTELEMEIQEVFLRFMASILKGYRTYLKPITQAPSEKATAADSLYDLQGFLKSRDRAHQKFYSQLTKTQIFIRFVEECTFVSDKDTGLAFFDDCIRKLFPSDKGTDKGTKVEGESSEDTRLMELDESQKSEHTVFVMPPEPPLEDGPDPPPKYSYKNFPSLRLELFDRPLELRPALSTRAAGSSVSSSPALLAKRTKQEIKMAYKLAKRFYSNPPQWAKCLCSHCYSLWFICLPARMRTAQSKPRAMQQAFNVLLKMRSSEVEVLDEVCYRVVMQLCGLWEMPVMAVRVLMEMKKAGVEPNAITYGYYNKAVLESPWPSRNRSGLFMWTKMRNLVRGVVQFKRALRGTTLQIGSSLKSTDLSASTAVGADGDRLSHGSVDGIVEEHILFSHSILVEDVIDSHYSTAAQSDQGYGSKDELPQEHTEEPVTSPPLSTNHTCSSKETNTKNGQDIASSVDSVVAVTEPPSPAEIAPCVASIVKPSTDIPVHFDSTAGRGETGVCTGKLFMSRKKSEDVALSVEDATVIPDSQLPQSGQQQRVKAFMERSCSFSAESKAGMLQKKSSLELSADHMGADAKILAAVFSGSQTPPPFASIAPFKEFKVEATLSPALQEEEQEEKQETKHESFTSAAEGVEKSIKRDNAAAESNSSERVGRTTGSAVSSSKAVERENVEMGADPLSLFASETEDTTSIQSQESTRTFPPLVSRNLADEIEMYMSLQSPLTARSPSMELQQGSNPESINCPDSPAVKQSLERRSSLPIGPVKVASSTEHPKPSPAVTRSKTFAVKSPKTPGSAGQRSSSLTALVKSSQNSSLGSVINSISGIKMDSLLVGPKVDMLKSGMKQAANVASKMWDAVASAYSYSDDEEDNLKPQYSFPARLEEQLLSGEGDTENTLPRGLMPSLGSNGLAHSNTSLGSSSGSSDTGQAHLSTPLTPGRSMRGADSERSEHSSSHHASTSSIYQNCALEVLMSSCSQCRSCEALVYDEEIMAGWTADDSNLNCTCPFCQVTFLPLLHVEFQDLRATPGFYLKTSTSGDSIHSSTTQPTTSSSSEVKVSSPSDPSDLMTFAESSEDSKSPVEMPVAQTGTPKILVPEPVHSDPLGLMERQAEKRNTLTRSNSMGGPLQNLDLIQRPNHGISTISLPNSLQEVSDTLGQKRPNPKPVSVPYLSPLVLRKELESLLENEGDQVIYTHKFLSQHPIIFWNLVWYFRRLDLPSILPGLILTSEHCNNGVKLPQTSLSQDSKQVYVQLLWDNVNLHQEPTEPLYQLWRTFLQRKGTLAPTDHQETRSLLNSIVRSIQMNDVYGPINMLIREIKKHPEVKRQRSVYREIMFLSLVALGRENIDIEAFNREYRLAYSQLSAEQLKVLSPIDRPPSNGVQWCLNSFGAPVI

Target ORF information:

RefSeq Version	XM_001919955.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919955.7

ORF Insert Sequence:

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GGTGTTTGCA CTGGTAAGCT CTTCATGTCC 
CGTAAAAAGA GTGAAGATGT GGCCTTGTCT GTGGAGGATG CTACTGTAAT CCCAGATTCA 
CAGCTTCCGC AGTCTGGACA GCAGCAAAGG GTAAAGGCCT TCATGGAGCG CAGCTGCAGT 
TTTAGTGCAG AGAGCAAAGC TGGCATGCTA CAGAAAAAAA GCAGTCTGGA GCTCAGTGCT 
GATCACATGG GCGCAGATGC AAAAATTCTT GCAGCAGTGT TCTCCGGCAG CCAGACTCCT 
CCTCCTTTTG CAAGCATTGC TCCGTTTAAA GAGTTCAAGG TGGAGGCTAC ACTAAGCCCT 
GCCCTCCAGG AGGAAGAACA AGAGGAAAAG CAGGAGACCA AACATGAATC CTTCACTTCT 
GCAGCAGAGG GAGTGGAGAA AAGTATAAAG CGGGATAATG CAGCAGCAGA ATCCAATAGC 
AGTGAACGAG TTGGGCGAAC CACAGGGAGT GCCGTCTCCT CCTCTAAAGC TGTGGAGAGG 
GAGAATGTCG AAATGGGAGC AGATCCACTC TCACTCTTTG CTTCAGAAAC TGAAGACACA 
ACCTCCATTC AGAGCCAGGA GTCCACACGG ACCTTTCCAC CTCTGGTGTC AAGGAATCTT 
GCAGATGAGA TTGAGATGTA CATGAGCCTC CAAAGCCCCC TGACTGCTAG ATCTCCTAGC 
ATGGAGTTGC AACAGGGTTC AAACCCTGAA TCCATAAACT GTCCAGACTC TCCAGCAGTT 
AAGCAGTCTT TAGAAAGAAG GTCAAGCCTT CCGATTGGCC CAGTCAAAGT GGCCAGTTCA 
ACGGAGCATC CAAAACCTAG TCCTGCTGTC ACTCGCTCAA AGACATTTGC TGTCAAATCC 
CCCAAGACCC CTGGCTCTGC AGGCCAGAGG TCGTCATCGC TGACTGCACT GGTAAAATCG 
TCACAGAACA GCTCACTAGG CTCTGTCATC AACTCCATCT CAGGCATCAA GATGGACTCT 
CTTCTGGTGG GCCCCAAAGT AGATATGCTT AAATCAGGCA TGAAGCAGGC AGCCAATGTG 
GCCAGTAAGA TGTGGGATGC AGTGGCTTCA GCCTACTCCT ACTCAGATGA TGAGGAGGAT 
AACTTAAAGC CCCAGTACAG TTTCCCAGCT AGGCTAGAAG AACAGTTGCT ATCTGGAGAG 
GGAGATACGG AGAACACTTT ACCCAGAGGA CTGATGCCTA GTCTGGGCTC AAACGGGCTC 
GCACACAGCA ATACCAGCCT GGGTAGCAGC AGTGGAAGCA GCGATACCGG CCAGGCACAC 
CTGTCAACTC CTTTGACTCC AGGTCGCTCA ATGAGAGGAG CAGACTCCGA GAGGTCTGAA 
CATAGTTCCT CTCATCATGC CAGCACCTCC AGCATTTACC AAAACTGTGC TTTAGAGGTT 
CTCATGTCCA GCTGCTCTCA GTGTCGCTCT TGTGAGGCAC TAGTGTATGA TGAGGAGATC 
ATGGCGGGCT GGACAGCAGA TGACTCCAAT CTAAACTGCA CCTGTCCCTT CTGTCAAGTC 
ACTTTCCTTC CTTTGCTCCA TGTTGAGTTC CAGGACCTGC GTGCTACTCC AGGCTTCTAC 
TTGAAGACGA GTACATCAGG AGACAGTATC CACAGCTCCA CCACACAACC AACCACTTCT 
AGCTCATCAG AAGTGAAAGT GAGCTCTCCT AGTGACCCTT CAGACCTCAT GACTTTCGCA 
GAATCTTCTG AGGACAGCAA GAGTCCTGTT GAGATGCCTG TTGCTCAAAC TGGAACCCCA 
AAGATCCTTG TACCTGAGCC TGTACATTCA GACCCCCTTG GTCTCATGGA GAGACAAGCT 
GAGAAACGGA ACACATTAAC ACGGAGCAAC AGTATGGGAG GACCACTGCA GAATTTGGAC 
TTGATTCAAA GACCCAATCA TGGCATATCC ACTATTAGCC TGCCCAACAG CTTACAAGAA 
GTGTCGGACA CGTTGGGTCA AAAGCGACCC AACCCAAAAC CAGTTTCTGT GCCGTATCTC 
AGTCCACTGG TTCTGCGCAA AGAATTGGAG TCGCTTTTAG AAAATGAAGG GGATCAGGTA 
ATCTATACAC ATAAGTTTCT TAGTCAGCAC CCCATCATTT TCTGGAACCT GGTGTGGTAT 
TTTCGTCGTT TAGACCTGCC CAGCATTCTG CCTGGACTCA TTCTCACTTC TGAACACTGC 
AACAATGGAG TAAAGCTGCC GCAGACATCT TTGTCTCAGG ACAGTAAGCA GGTGTACGTG 
CAGCTGTTGT GGGATAATGT CAACTTACAT CAGGAGCCCA CCGAGCCCCT CTATCAGCTC 
TGGAGGACCT TCTTGCAAAG GAAGGGAACT CTAGCCCCAA CTGACCACCA GGAGACTCGG 
TCTTTGCTAA ACAGCATTGT ACGCAGCATT CAGATGAACG ATGTTTACGG CCCCATCAAT 
ATGCTCATCC GGGAGATCAA GAAACATCCA GAAGTTAAAC GCCAGAGGAG TGTCTACAGG 
GAAATTATGT TTCTGTCACT TGTTGCCTTA GGAAGGGAGA ACATTGATAT TGAGGCCTTT 
AATCGTGAAT ACCGTCTTGC TTACAGCCAG CTCAGCGCAG AACAGCTTAA AGTCCTGTCC 
CCTATTGACA GACCACCTAG CAACGGTGTG CAGTGGTGCC TCAATTCCTT TGGAGCCCCA 
GTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4C ( XM_001919955.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001919990.2 Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

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CloneID	ODa13592
Clone ID Related Accession (Same CDS sequence)	XM_001919955.7 , XM_001919955.6
Accession Version	XM_001919955.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 4C isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919955.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GGTGTTTGCA CTGGTAAGCT CTTCATGTCC 
CGTAAAAAGA GTGAAGATGT GGCCTTGTCT GTGGAGGATG CTACTGTAAT CCCAGATTCA 
CAGCTTCCGC AGTCTGGACA GCAGCAAAGG GTAAAGGCCT TCATGGAGCG CAGCTGCAGT 
TTTAGTGCAG AGAGCAAAGC TGGCATGCTA CAGAAAAAAA GCAGTCTGGA GCTCAGTGCT 
GATCACATGG GCGCAGATGC AAAAATTCTT GCAGCAGTGT TCTCCGGCAG CCAGACTCCT 
CCTCCTTTTG CAAGCATTGC TCCGTTTAAA GAGTTCAAGG TGGAGGCTAC ACTAAGCCCT 
GCCCTCCAGG AGGAAGAACA AGAGGAAAAG CAGGAGACCA AACATGAATC CTTCACTTCT 
GCAGCAGAGG GAGTGGAGAA AAGTATAAAG CGGGATAATG CAGCAGCAGA ATCCAATAGC 
AGTGAACGAG TTGGGCGAAC CACAGGGAGT GCCGTCTCCT CCTCTAAAGC TGTGGAGAGG 
GAGAATGTCG AAATGGGAGC AGATCCACTC TCACTCTTTG CTTCAGAAAC TGAAGACACA 
ACCTCCATTC AGAGCCAGGA GTCCACACGG ACCTTTCCAC CTCTGGTGTC AAGGAATCTT 
GCAGATGAGA TTGAGATGTA CATGAGCCTC CAAAGCCCCC TGACTGCTAG ATCTCCTAGC 
ATGGAGTTGC AACAGGGTTC AAACCCTGAA TCCATAAACT GTCCAGACTC TCCAGCAGTT 
AAGCAGTCTT TAGAAAGAAG GTCAAGCCTT CCGATTGGCC CAGTCAAAGT GGCCAGTTCA 
ACGGAGCATC CAAAACCTAG TCCTGCTGTC ACTCGCTCAA AGACATTTGC TGTCAAATCC 
CCCAAGACCC CTGGCTCTGC AGGCCAGAGG TCGTCATCGC TGACTGCACT GGTAAAATCG 
TCACAGAACA GCTCACTAGG CTCTGTCATC AACTCCATCT CAGGCATCAA GATGGACTCT 
CTTCTGGTGG GCCCCAAAGT AGATATGCTT AAATCAGGCA TGAAGCAGGC AGCCAATGTG 
GCCAGTAAGA TGTGGGATGC AGTGGCTTCA GCCTACTCCT ACTCAGATGA TGAGGAGGAT 
AACTTAAAGC CCCAGTACAG TTTCCCAGCT AGGCTAGAAG AACAGTTGCT ATCTGGAGAG 
GGAGATACGG AGAACACTTT ACCCAGAGGA CTGATGCCTA GTCTGGGCTC AAACGGGCTC 
GCACACAGCA ATACCAGCCT GGGTAGCAGC AGTGGAAGCA GCGATACCGG CCAGGCACAC 
CTGTCAACTC CTTTGACTCC AGGTCGCTCA ATGAGAGGAG CAGACTCCGA GAGGTCTGAA 
CATAGTTCCT CTCATCATGC CAGCACCTCC AGCATTTACC AAAACTGTGC TTTAGAGGTT 
CTCATGTCCA GCTGCTCTCA GTGTCGCTCT TGTGAGGCAC TAGTGTATGA TGAGGAGATC 
ATGGCGGGCT GGACAGCAGA TGACTCCAAT CTAAACTGCA CCTGTCCCTT CTGTCAAGTC 
ACTTTCCTTC CTTTGCTCCA TGTTGAGTTC CAGGACCTGC GTGCTACTCC AGGCTTCTAC 
TTGAAGACGA GTACATCAGG AGACAGTATC CACAGCTCCA CCACACAACC AACCACTTCT 
AGCTCATCAG AAGTGAAAGT GAGCTCTCCT AGTGACCCTT CAGACCTCAT GACTTTCGCA 
GAATCTTCTG AGGACAGCAA GAGTCCTGTT GAGATGCCTG TTGCTCAAAC TGGAACCCCA 
AAGATCCTTG TACCTGAGCC TGTACATTCA GACCCCCTTG GTCTCATGGA GAGACAAGCT 
GAGAAACGGA ACACATTAAC ACGGAGCAAC AGTATGGGAG GACCACTGCA GAATTTGGAC 
TTGATTCAAA GACCCAATCA TGGCATATCC ACTATTAGCC TGCCCAACAG CTTACAAGAA 
GTGTCGGACA CGTTGGGTCA AAAGCGACCC AACCCAAAAC CAGTTTCTGT GCCGTATCTC 
AGTCCACTGG TTCTGCGCAA AGAATTGGAG TCGCTTTTAG AAAATGAAGG GGATCAGGTA 
ATCTATACAC ATAAGTTTCT TAGTCAGCAC CCCATCATTT TCTGGAACCT GGTGTGGTAT 
TTTCGTCGTT TAGACCTGCC CAGCATTCTG CCTGGACTCA TTCTCACTTC TGAACACTGC 
AACAATGGAG TAAAGCTGCC GCAGACATCT TTGTCTCAGG ACAGTAAGCA GGTGTACGTG 
CAGCTGTTGT GGGATAATGT CAACTTACAT CAGGAGCCCA CCGAGCCCCT CTATCAGCTC 
TGGAGGACCT TCTTGCAAAG GAAGGGAACT CTAGCCCCAA CTGACCACCA GGAGACTCGG 
TCTTTGCTAA ACAGCATTGT ACGCAGCATT CAGATGAACG ATGTTTACGG CCCCATCAAT 
ATGCTCATCC GGGAGATCAA GAAACATCCA GAAGTTAAAC GCCAGAGGAG TGTCTACAGG 
GAAATTATGT TTCTGTCACT TGTTGCCTTA GGAAGGGAGA ACATTGATAT TGAGGCCTTT 
AATCGTGAAT ACCGTCTTGC TTACAGCCAG CTCAGCGCAG AACAGCTTAA AGTCCTGTCC 
CCTATTGACA GACCACCTAG CAACGGTGTG CAGTGGTGCC TCAATTCCTT TGGAGCCCCA 
GTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919990.2
CDS	484..6312
Translation	MIEDKGHRVTDYFVVAGLTDKSTPLEQDISEAKSSGPKAPITDLAVINKSSGETVPEGFTCIESTYSGQPANLNHGSLKSPELFLCYKRGRGKPPLIDIGVLYEGKERLIQGCEVIEATPFGRCANVNNSSATSQRIFITYRRAPPVQPQNSLAVTDICVIITNKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVSSSNSIAYKAGLIFRYPEEDYESFPLSESVPLFCLPMGAKIECWAPNTRYPLPVFSTFVLTNSNGEKVYGAGIQFYEPYPVDGLSEKQKIQLGLVSAVDKKVLPNRTVNTNKCICLLSRWPFFEAFRKFLMFLYKLSANGVSPLPIEKHISHFMHNVSFPSPQRPRILVQLSAHDTLILSQPVCTPLPLSGADYSTLLMNLGPENCATLLHFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLNAPCPFIVGVDSRYFDLYDPPPDVVCVDLDTNTIYLSDEKRHSNWKNLPKKPSKSLISFLGSLHHQLATVRRTATEGLAVEMTPIEADFTWHKKKTELEMEIQEVFLRFMASILKGYRTYLKPITQAPSEKATAADSLYDLQGFLKSRDRAHQKFYSQLTKTQIFIRFVEECTFVSDKDTGLAFFDDCIRKLFPSDKGTDKGTKVEGESSEDTRLMELDESQKSEHTVFVMPPEPPLEDGPDPPPKYSYKNFPSLRLELFDRPLELRPALSTRAAGSSVSSSPALLAKRTKQEIKMAYKLAKRFYSNPPQWAKCLCSHCYSLWFICLPARMRTAQSKPRAMQQAFNVLLKMRSSEVEVLDEVCYRVVMQLCGLWEMPVMAVRVLMEMKKAGVEPNAITYGYYNKAVLESPWPSRNRSGLFMWTKMRNLVRGVVQFKRALRGTTLQIGSSLKSTDLSASTAVGADGDRLSHGSVDGIVEEHILFSHSILVEDVIDSHYSTAAQSDQGYGSKDELPQEHTEEPVTSPPLSTNHTCSSKETNTKNGQDIASSVDSVVAVTEPPSPAEIAPCVASIVKPSTDIPVHFDSTAGRGETGVCTGKLFMSRKKSEDVALSVEDATVIPDSQLPQSGQQQRVKAFMERSCSFSAESKAGMLQKKSSLELSADHMGADAKILAAVFSGSQTPPPFASIAPFKEFKVEATLSPALQEEEQEEKQETKHESFTSAAEGVEKSIKRDNAAAESNSSERVGRTTGSAVSSSKAVERENVEMGADPLSLFASETEDTTSIQSQESTRTFPPLVSRNLADEIEMYMSLQSPLTARSPSMELQQGSNPESINCPDSPAVKQSLERRSSLPIGPVKVASSTEHPKPSPAVTRSKTFAVKSPKTPGSAGQRSSSLTALVKSSQNSSLGSVINSISGIKMDSLLVGPKVDMLKSGMKQAANVASKMWDAVASAYSYSDDEEDNLKPQYSFPARLEEQLLSGEGDTENTLPRGLMPSLGSNGLAHSNTSLGSSSGSSDTGQAHLSTPLTPGRSMRGADSERSEHSSSHHASTSSIYQNCALEVLMSSCSQCRSCEALVYDEEIMAGWTADDSNLNCTCPFCQVTFLPLLHVEFQDLRATPGFYLKTSTSGDSIHSSTTQPTTSSSSEVKVSSPSDPSDLMTFAESSEDSKSPVEMPVAQTGTPKILVPEPVHSDPLGLMERQAEKRNTLTRSNSMGGPLQNLDLIQRPNHGISTISLPNSLQEVSDTLGQKRPNPKPVSVPYLSPLVLRKELESLLENEGDQVIYTHKFLSQHPIIFWNLVWYFRRLDLPSILPGLILTSEHCNNGVKLPQTSLSQDSKQVYVQLLWDNVNLHQEPTEPLYQLWRTFLQRKGTLAPTDHQETRSLLNSIVRSIQMNDVYGPINMLIREIKKHPEVKRQRSVYREIMFLSLVALGRENIDIEAFNREYRLAYSQLSAEQLKVLSPIDRPPSNGVQWCLNSFGAPVI

Target ORF information:

RefSeq Version	XM_001919955.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 4C (dennd4c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919955.6

ORF Insert Sequence:

ATGATTGAGG ATAAGGGTCA CCGCGTGACG GACTACTTCG TTGTGGCTGG CCTGACAGAC 
AAGTCTACAC CACTAGAGCA GGATATATCT GAAGCCAAGT CCAGTGGGCC CAAGGCACCA 
ATCACAGACC TTGCTGTCAT CAACAAGTCT TCTGGAGAAA CTGTGCCTGA AGGTTTCACA 
TGCATCGAGA GCACCTACAG CGGCCAGCCG GCCAATCTCA ACCATGGCAG CCTCAAGAGC 
CCAGAACTCT TCCTATGTTA CAAGAGGGGT CGAGGGAAGC CCCCGCTCAT TGACATTGGG 
GTCCTGTATG AAGGTAAGGA GCGCCTCATT CAGGGTTGTG AGGTCATAGA GGCCACACCG 
TTTGGCCGCT GTGCAAATGT CAATAACAGC TCTGCCACAT CCCAGCGCAT ATTCATCACT 
TACCGCCGAG CGCCTCCTGT CCAACCCCAG AACTCACTGG CTGTGACCGA CATCTGCGTC 
ATTATCACAA ACAAGGGCGA GACTCCTCCT CATACCTTCT GCAAGGTAGA CAAGAACCTT 
AACTGTGGCA TGTGGGGATC AAGTGTGTTT TTATGCTACA AGAAGTCTGT GTCTTCTTCT 
AATTCCATCG CTTATAAGGC TGGTCTGATC TTTCGATATC CTGAGGAGGA CTACGAGTCA 
TTTCCTCTCT CTGAGTCTGT ACCCCTCTTT TGCCTGCCTA TGGGGGCCAA GATTGAATGC 
TGGGCTCCAA ACACACGCTA CCCTTTACCC GTTTTCTCCA CATTTGTCCT CACCAACTCC 
AATGGAGAGA AGGTATATGG AGCTGGAATA CAGTTTTACG AGCCTTATCC TGTGGATGGC 
CTCAGCGAGA AACAGAAGAT CCAGCTGGGT CTTGTCTCTG CAGTAGACAA GAAAGTCCTT 
CCCAACCGCA CAGTCAACAC CAACAAGTGC ATCTGCCTGC TGTCCCGCTG GCCTTTCTTT 
GAGGCCTTTA GAAAGTTCCT CATGTTTCTT TATAAGCTGT CGGCCAATGG AGTCAGCCCT 
CTACCTATTG AGAAACATAT ATCCCATTTC ATGCACAATG TATCCTTCCC GTCTCCTCAG 
AGGCCAAGAA TACTGGTTCA GCTGTCTGCA CATGACACCT TAATCCTGTC CCAACCTGTT 
TGTACACCTT TACCACTGAG TGGAGCAGAC TACAGTACAC TGCTTATGAA TCTAGGCCCA 
GAAAACTGTG CCACACTGCT TCACTTTGTC CTGCTGGAAA GTAAGATCCT GTTGCACTCC 
CTTCGACCAG CTGTTCTGAC TGGAGTGGCT GAGGCTGTTG TGGCAATGAT CTTCCCCTTC 
CAGTGGCAAT GTCCATATAT TCCTCTTTGC CCTCTGTCTT TGGCGGGGGT TCTCAATGCA 
CCATGTCCTT TCATTGTGGG CGTTGACTCA AGATACTTTG ACCTCTACGA TCCCCCTCCT 
GACGTAGTGT GTGTGGACTT GGACACCAAC ACTATATATT TGTCTGATGA GAAACGACAC 
AGTAACTGGA AGAATCTCCC AAAGAAGCCC TCCAAGAGTC TTATCAGTTT CTTAGGAAGC 
TTGCACCACC AGCTTGCCAC TGTTCGGCGT ACAGCCACAG AAGGCTTGGC AGTGGAGATG 
ACCCCTATCG AAGCTGACTT CACCTGGCAT AAGAAAAAGA CTGAGCTGGA GATGGAGATT 
CAAGAAGTGT TCCTGCGCTT TATGGCCTCC ATACTGAAGG GCTACCGAAC CTACCTCAAA 
CCCATCACAC AAGCACCTTC TGAAAAAGCT ACTGCTGCAG ATTCGCTCTA CGACCTGCAA 
GGATTCTTGA AGAGCAGAGA TCGTGCTCAC CAGAAGTTCT ACTCTCAGCT CACCAAGACT 
CAGATCTTCA TCCGCTTTGT TGAGGAATGT ACTTTTGTCA GTGACAAAGA CACCGGCCTG 
GCTTTCTTTG ATGACTGCAT TCGAAAACTT TTTCCTTCTG ACAAAGGGAC AGACAAGGGT 
ACAAAGGTTG AAGGCGAGTC TTCTGAGGAC ACCAGGCTGA TGGAACTGGA CGAGTCCCAG 
AAGAGTGAGC ACACAGTTTT TGTCATGCCT CCTGAACCCC CTTTGGAAGA TGGTCCTGAC 
CCCCCTCCCA AGTACAGTTA TAAGAATTTC CCTAGTTTAC GGTTGGAGTT GTTTGATCGT 
CCACTAGAAC TGAGGCCAGC ATTAAGCACA AGAGCTGCAG GGTCGAGTGT GTCCAGCAGT 
CCTGCCCTTC TGGCCAAAAG AACTAAACAG GAAATCAAAA TGGCCTACAA ATTGGCGAAG 
CGTTTCTACT CCAATCCACC CCAGTGGGCA AAGTGTCTGT GTAGTCACTG CTATAGTCTG 
TGGTTCATCT GTCTTCCTGC TCGCATGCGT ACTGCACAGT CCAAGCCCCG CGCCATGCAG 
CAGGCCTTTA ATGTTCTGCT CAAGATGAGG AGCTCTGAGG TGGAAGTGCT TGACGAGGTG 
TGTTACCGTG TGGTGATGCA GTTATGTGGT CTGTGGGAGA TGCCCGTCAT GGCTGTGCGT 
GTCTTAATGG AGATGAAAAA GGCTGGAGTT GAACCCAATG CTATTACTTA TGGATACTAC 
AACAAGGCTG TTCTGGAAAG TCCTTGGCCA AGCCGCAACC GTAGCGGTCT CTTCATGTGG 
ACCAAAATGA GGAACCTTGT GCGAGGAGTG GTGCAGTTTA AACGGGCATT ACGTGGGACC 
ACTCTTCAAA TAGGATCTTC TCTGAAAAGC ACAGATCTTT CAGCAAGTAC AGCAGTAGGT 
GCTGATGGAG ACAGACTGAG TCATGGAAGC GTAGACGGAA TTGTCGAGGA ACACATTCTC 
TTCTCCCACA GCATTTTAGT TGAGGATGTT ATTGACAGTC ACTACAGTAC AGCTGCTCAG 
TCTGATCAGG GTTATGGATC TAAAGATGAA TTACCACAGG AACATACAGA AGAACCGGTA 
ACCTCACCAC CTCTGTCTAC CAACCACACC TGTAGTAGCA AAGAAACCAA CACCAAAAAT 
GGACAAGATA TTGCCAGTTC TGTGGACAGT GTTGTAGCTG TAACAGAGCC CCCTAGCCCT 
GCTGAGATAG CACCATGTGT AGCCAGCATT GTAAAACCCT CTACTGATAT CCCTGTCCAC 
TTTGACAGCA CTGCAGGCAG GGGAGAAACT GGTGTTTGCA CTGGTAAGCT CTTCATGTCC 
CGTAAAAAGA GTGAAGATGT GGCCTTGTCT GTGGAGGATG CTACTGTAAT CCCAGATTCA 
CAGCTTCCGC AGTCTGGACA GCAGCAAAGG GTAAAGGCCT TCATGGAGCG CAGCTGCAGT 
TTTAGTGCAG AGAGCAAAGC TGGCATGCTA CAGAAAAAAA GCAGTCTGGA GCTCAGTGCT 
GATCACATGG GCGCAGATGC AAAAATTCTT GCAGCAGTGT TCTCCGGCAG CCAGACTCCT 
CCTCCTTTTG CAAGCATTGC TCCGTTTAAA GAGTTCAAGG TGGAGGCTAC ACTAAGCCCT 
GCCCTCCAGG AGGAAGAACA AGAGGAAAAG CAGGAGACCA AACATGAATC CTTCACTTCT 
GCAGCAGAGG GAGTGGAGAA AAGTATAAAG CGGGATAATG CAGCAGCAGA ATCCAATAGC 
AGTGAACGAG TTGGGCGAAC CACAGGGAGT GCCGTCTCCT CCTCTAAAGC TGTGGAGAGG 
GAGAATGTCG AAATGGGAGC AGATCCACTC TCACTCTTTG CTTCAGAAAC TGAAGACACA 
ACCTCCATTC AGAGCCAGGA GTCCACACGG ACCTTTCCAC CTCTGGTGTC AAGGAATCTT 
GCAGATGAGA TTGAGATGTA CATGAGCCTC CAAAGCCCCC TGACTGCTAG ATCTCCTAGC 
ATGGAGTTGC AACAGGGTTC AAACCCTGAA TCCATAAACT GTCCAGACTC TCCAGCAGTT 
AAGCAGTCTT TAGAAAGAAG GTCAAGCCTT CCGATTGGCC CAGTCAAAGT GGCCAGTTCA 
ACGGAGCATC CAAAACCTAG TCCTGCTGTC ACTCGCTCAA AGACATTTGC TGTCAAATCC 
CCCAAGACCC CTGGCTCTGC AGGCCAGAGG TCGTCATCGC TGACTGCACT GGTAAAATCG 
TCACAGAACA GCTCACTAGG CTCTGTCATC AACTCCATCT CAGGCATCAA GATGGACTCT 
CTTCTGGTGG GCCCCAAAGT AGATATGCTT AAATCAGGCA TGAAGCAGGC AGCCAATGTG 
GCCAGTAAGA TGTGGGATGC AGTGGCTTCA GCCTACTCCT ACTCAGATGA TGAGGAGGAT 
AACTTAAAGC CCCAGTACAG TTTCCCAGCT AGGCTAGAAG AACAGTTGCT ATCTGGAGAG 
GGAGATACGG AGAACACTTT ACCCAGAGGA CTGATGCCTA GTCTGGGCTC AAACGGGCTC 
GCACACAGCA ATACCAGCCT GGGTAGCAGC AGTGGAAGCA GCGATACCGG CCAGGCACAC 
CTGTCAACTC CTTTGACTCC AGGTCGCTCA ATGAGAGGAG CAGACTCCGA GAGGTCTGAA 
CATAGTTCCT CTCATCATGC CAGCACCTCC AGCATTTACC AAAACTGTGC TTTAGAGGTT 
CTCATGTCCA GCTGCTCTCA GTGTCGCTCT TGTGAGGCAC TAGTGTATGA TGAGGAGATC 
ATGGCGGGCT GGACAGCAGA TGACTCCAAT CTAAACTGCA CCTGTCCCTT CTGTCAAGTC 
ACTTTCCTTC CTTTGCTCCA TGTTGAGTTC CAGGACCTGC GTGCTACTCC AGGCTTCTAC 
TTGAAGACGA GTACATCAGG AGACAGTATC CACAGCTCCA CCACACAACC AACCACTTCT 
AGCTCATCAG AAGTGAAAGT GAGCTCTCCT AGTGACCCTT CAGACCTCAT GACTTTCGCA 
GAATCTTCTG AGGACAGCAA GAGTCCTGTT GAGATGCCTG TTGCTCAAAC TGGAACCCCA 
AAGATCCTTG TACCTGAGCC TGTACATTCA GACCCCCTTG GTCTCATGGA GAGACAAGCT 
GAGAAACGGA ACACATTAAC ACGGAGCAAC AGTATGGGAG GACCACTGCA GAATTTGGAC 
TTGATTCAAA GACCCAATCA TGGCATATCC ACTATTAGCC TGCCCAACAG CTTACAAGAA 
GTGTCGGACA CGTTGGGTCA AAAGCGACCC AACCCAAAAC CAGTTTCTGT GCCGTATCTC 
AGTCCACTGG TTCTGCGCAA AGAATTGGAG TCGCTTTTAG AAAATGAAGG GGATCAGGTA 
ATCTATACAC ATAAGTTTCT TAGTCAGCAC CCCATCATTT TCTGGAACCT GGTGTGGTAT 
TTTCGTCGTT TAGACCTGCC CAGCATTCTG CCTGGACTCA TTCTCACTTC TGAACACTGC 
AACAATGGAG TAAAGCTGCC GCAGACATCT TTGTCTCAGG ACAGTAAGCA GGTGTACGTG 
CAGCTGTTGT GGGATAATGT CAACTTACAT CAGGAGCCCA CCGAGCCCCT CTATCAGCTC 
TGGAGGACCT TCTTGCAAAG GAAGGGAACT CTAGCCCCAA CTGACCACCA GGAGACTCGG 
TCTTTGCTAA ACAGCATTGT ACGCAGCATT CAGATGAACG ATGTTTACGG CCCCATCAAT 
ATGCTCATCC GGGAGATCAA GAAACATCCA GAAGTTAAAC GCCAGAGGAG TGTCTACAGG 
GAAATTATGT TTCTGTCACT TGTTGCCTTA GGAAGGGAGA ACATTGATAT TGAGGCCTTT 
AATCGTGAAT ACCGTCTTGC TTACAGCCAG CTCAGCGCAG AACAGCTTAA AGTCCTGTCC 
CCTATTGACA GACCACCTAG CAACGGTGTG CAGTGGTGCC TCAATTCCTT TGGAGCCCCA 
GTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DENND4C cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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