atp5fa1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	atp5fa1
Entrez Gene ID	553755
Full Name	ATP synthase F1 subunit alpha
Synonyms	atp5a1,zgc:154103
General protein information	Preferred Names ATP synthase F1 subunit alpha Names ATP synthase subunit alpha, mitochondrial ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle mitochondrial ATP synthase alpha subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077355.1	NP_001070823.1	ATP synthase subunit alpha, mitochondrial

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07752	XP_003712965.1
Neurospora crassa	NCU02514	XP_965645.1
Schizosaccharomyces pombe (fission yeast)	atp1	NP_594919.1
Pan troglodytes (chimpanzee)	ATP5A1	NP_001129208.1
Mus musculus (house mouse)	Atp5a1	NP_031531.1
Saccharomyces cerevisiae (baker's yeast)	ATP1	NP_009453.2
Canis lupus familiaris (dog)	ATP5A1	XP_852877.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005134	XP_314018.2
Macaca mulatta (Rhesus monkey)	ATP5A1	XP_001089681.2
Bos taurus (cattle)	ATP5A1	NP_777109.1
Caenorhabditis elegans (roundworm)	H28O16.1	NP_001021526.1
Arabidopsis thaliana (thale cress)	AT2G07698	NP_178788.1
Arabidopsis thaliana (thale cress)	atp1	NP_085571.2
Kluyveromyces lactis	KLLA0E06667g	XP_454248.1
Homo sapiens (human)	ATP5A1	NP_004037.1
Rattus norvegicus (Norway rat)	Atp5a1	NP_075581.1
Danio rerio (zebrafish)	atp5a1	NP_001070823.1
Gallus gallus (chicken)	ATP5A1	XP_004949099.1
Gallus gallus (chicken)	ATP5A1W	XP_429118.3
Drosophila melanogaster (fruit fly)	blw	NP_726243.1
Xenopus tropicalis (tropical clawed frog)	atp5a1	NP_001025610.1
Eremothecium gossypii	AGOS_AGL272C	NP_986395.1

Gene Ontology
Bibliography

Related articles in PubMed

Mechanistic studies on ketamine-induced mitochondrial toxicity in zebrafish embryos.
Robinson BL, Dumas M, Ali SF, Paule MG, Gu Q, Kanungo J
Neurotoxicology and teratology6963-72(2018 Sep - Oct)

A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways.
Piragyte I, Clapes T, Polyzou A, Klein Geltink RI, Lefkopoulos S, Yin N, Cauchy P, Curtis JD, Klaeylé L, Langa X, Beckmann CCA, Wlodarski MW, Müller P, Van Essen D, Rambold A, Kapp FG, Mione M, Buescher JM, Pearce EL, Polyzos A, Trompouki E
Nature communications9(1)3090(2018 08)

Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos.
Parolini M, Bini L, Magni S, Rizzo A, Ghilardi A, Landi C, Armini A, Del Giacco L, Binelli A
Environmental pollution (Barking, Essex : 1987)232603-614(2018 Jan)

Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy.
Nagata Y, Yamagishi M, Konno T, Nakanishi C, Asano Y, Ito S, Nakajima Y, Seguchi O, Fujino N, Kawashiri MA, Takashima S, Kitakaze M, Hayashi K
Scientific reports7(1)17417(2017 12)

Altered glycometabolism in zebrafish exposed to thifluzamide.
Yang Y, Liu W, Li D, Qian L, Fu B, Wang C
Chemosphere18389-96(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following atp5fa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atp5fa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37262	NM_001077355.1 Latest version!	Danio rerio ATP synthase F1 subunit alpha (atp5fa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atp5fa1 ( NM_001077355.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070823.1 Danio rerio ATP synthase F1 subunit alpha (atp5fa1), mRNA.

Price & Availability↑

CloneID	ODa37262
Clone ID Related Accession (Same CDS sequence)	NM_001077355.1
Accession Version	NM_001077355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	ATP synthase subunit alpha, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124811.1. On Oct 22, 2006 this sequence version replaced XM_693400.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC124811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTTTCCG TTCGCGTCGC GGCGGCTCTG GCCCGCACTC TTCCACGACG GGCCGGATTC 
GTTTCCAAGA ATGTTGCTGC AGCATGCGTT GGAGCCAAGA ACCTGCACAC CGCCAGACCA 
TGGCTGCAGA AGACAGGCAC GGCGGAGGTG TCCAGCATTC TGGAGGAGAA GATTCTTGGA 
GCCGACACTG GAGCTGAACT GGAGGAGACC GGCCGCGTGC TGTCCATTGG TGATGGTATC 
GCTCGTGTTT ACGGGCTGAG GAACGTGCAG GCTGAAGAGA TGGTGGAGTT CTCCTCTGGT 
CTGAAGGGTA TGTCTCTGAA CTTGGAGCCT GATAACGTTG GTGTTGTGGT GTTCGGTAAT 
GACAAGCTGA TCAAGGAGGG AGACATCGTC AAGAGAACAG GGGCTATTGT GGACGTTCCT 
GTCGGAGAGG AGCTGCTCGG ACGTGTTGTG GATGCTCTGG GAAACCCCAT TGATGGCAAG 
GGACCTCTGG GCTCCAAAGA GCGCAGGCGT GTGGGTCTGA AGGCCCCTGG GATCATCCCT 
CGTATCTCTG TGAGGGAGCC CATGCAGACT GGCATCAAAG CTGTGGACAG TCTGGTGCCC 
ATCGGCCGTG GCCAGAGAGA GCTGATCATT GGTGACAGGC AGACTGGCAA AACCGCCATT 
GCCATCGACA CCATCATCAA CCAGAAGCGC TTCAATGAAG GCACTGAGGA GAAAAAGAAG 
CTGTACTGCA TCTACGTGGC CATCGGTCAG AAGAGATCCA CTGTGGCCCA GCTGGTGAAG 
AGGCTGACAG ATGCTGATGC CATGAAATAC ACCATCGTGG TGTCGGCCAC CGCATCTGAC 
GCCGCTCCAC TGCAGTACCT GGCTCCATAC TCCGGCTGCT CCATGGGGGA ATACTTCAGA 
GACAACGGCA AACACGCTCT CATCATCTAT GATGATCTGT CCAAACAGGC TGTTGCCTAC 
CGTCAGATGT CCCTGCTGCT GCGTCGTCCC CCAGGTCGTG AGGCCTACCC CGGTGACGTC 
TTCTACCTGC ACTCCCGTCT GCTGGAGAGA GCAGCCAAAA TGAACGACAA CTTCGGTGGT 
GGATCCCTGA CCGCCCTGCC CGTCATCGAG ACCCAGGCCG GAGACGTGTC CGCTTACATT 
CCCACCAACG TCATCTCCAT CACTGACGGA CAGATTTTCT TGGAGACTGA GCTGTTCTAC 
AAGGGTATCC GTCCCGCTAT CAATGTGGGT CTGTCTGTGT CCCGTGTCGG TTCTGCTGCT 
CAGACCAGGG CCATGAAGCA GGTGGCTGGT ACCATGAAGC TGGAGTTGGC TCAGTATCGT 
GAGGTTGCTG CTTTCGCTCA GTTTGGTTCA GATCTGGACG CTGCCACACA GCAACTGCTG 
AACAGAGGAG TGAGACTCAC TGAGCTGCTC AAGCAGGGAC AGTACTCTCC CATGGCCATT 
GAGGAGCAGG TGGCTGTCAT TTACGCTGGT GTGAGAGGAC ACTTGGACAA GATGGAGCCC 
AGCAAGATCA CCAAATTCGA GAAGGCCTTC CTCCAGCACG TTATCAGCCA GCACCAGGAT 
CTGCTCGCAG CCATCAGGAG CGACGGTAAG ATTTCAGAGG CCTCTGACGC AAAACTCAAG 
GAGATCGTGC TCAACTTCCT CTCGAGCTTC GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070823.1
CDS	52..1707
Translation	MLSVRVAAALARTLPRRAGFVSKNVAAACVGAKNLHTARPWLQKTGTAEVSSILEEKILGADTGAELEETGRVLSIGDGIARVYGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNPIDGKGPLGSKERRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTAIAIDTIINQKRFNEGTEEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDNFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLNRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGHLDKMEPSKITKFEKAFLQHVISQHQDLLAAIRSDGKISEASDAKLKEIVLNFLSSFE

Target ORF information:

RefSeq Version	NM_001077355.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATP synthase F1 subunit alpha (atp5fa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077355.1

ORF Insert Sequence:

ATGCTTTCCG TTCGCGTCGC GGCGGCTCTG GCCCGCACTC TTCCACGACG GGCCGGATTC 
GTTTCCAAGA ATGTTGCTGC AGCATGCGTT GGAGCCAAGA ACCTGCACAC CGCCAGACCA 
TGGCTGCAGA AGACAGGCAC GGCGGAGGTG TCCAGCATTC TGGAGGAGAA GATTCTTGGA 
GCCGACACTG GAGCTGAACT GGAGGAGACC GGCCGCGTGC TGTCCATTGG TGATGGTATC 
GCTCGTGTTT ACGGGCTGAG GAACGTGCAG GCTGAAGAGA TGGTGGAGTT CTCCTCTGGT 
CTGAAGGGTA TGTCTCTGAA CTTGGAGCCT GATAACGTTG GTGTTGTGGT GTTCGGTAAT 
GACAAGCTGA TCAAGGAGGG AGACATCGTC AAGAGAACAG GGGCTATTGT GGACGTTCCT 
GTCGGAGAGG AGCTGCTCGG ACGTGTTGTG GATGCTCTGG GAAACCCCAT TGATGGCAAG 
GGACCTCTGG GCTCCAAAGA GCGCAGGCGT GTGGGTCTGA AGGCCCCTGG GATCATCCCT 
CGTATCTCTG TGAGGGAGCC CATGCAGACT GGCATCAAAG CTGTGGACAG TCTGGTGCCC 
ATCGGCCGTG GCCAGAGAGA GCTGATCATT GGTGACAGGC AGACTGGCAA AACCGCCATT 
GCCATCGACA CCATCATCAA CCAGAAGCGC TTCAATGAAG GCACTGAGGA GAAAAAGAAG 
CTGTACTGCA TCTACGTGGC CATCGGTCAG AAGAGATCCA CTGTGGCCCA GCTGGTGAAG 
AGGCTGACAG ATGCTGATGC CATGAAATAC ACCATCGTGG TGTCGGCCAC CGCATCTGAC 
GCCGCTCCAC TGCAGTACCT GGCTCCATAC TCCGGCTGCT CCATGGGGGA ATACTTCAGA 
GACAACGGCA AACACGCTCT CATCATCTAT GATGATCTGT CCAAACAGGC TGTTGCCTAC 
CGTCAGATGT CCCTGCTGCT GCGTCGTCCC CCAGGTCGTG AGGCCTACCC CGGTGACGTC 
TTCTACCTGC ACTCCCGTCT GCTGGAGAGA GCAGCCAAAA TGAACGACAA CTTCGGTGGT 
GGATCCCTGA CCGCCCTGCC CGTCATCGAG ACCCAGGCCG GAGACGTGTC CGCTTACATT 
CCCACCAACG TCATCTCCAT CACTGACGGA CAGATTTTCT TGGAGACTGA GCTGTTCTAC 
AAGGGTATCC GTCCCGCTAT CAATGTGGGT CTGTCTGTGT CCCGTGTCGG TTCTGCTGCT 
CAGACCAGGG CCATGAAGCA GGTGGCTGGT ACCATGAAGC TGGAGTTGGC TCAGTATCGT 
GAGGTTGCTG CTTTCGCTCA GTTTGGTTCA GATCTGGACG CTGCCACACA GCAACTGCTG 
AACAGAGGAG TGAGACTCAC TGAGCTGCTC AAGCAGGGAC AGTACTCTCC CATGGCCATT 
GAGGAGCAGG TGGCTGTCAT TTACGCTGGT GTGAGAGGAC ACTTGGACAA GATGGAGCCC 
AGCAAGATCA CCAAATTCGA GAAGGCCTTC CTCCAGCACG TTATCAGCCA GCACCAGGAT 
CTGCTCGCAG CCATCAGGAG CGACGGTAAG ATTTCAGAGG CCTCTGACGC AAAACTCAAG 
GAGATCGTGC TCAACTTCCT CTCGAGCTTC GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanistic studies on ketamine-induced mitochondrial toxicity in zebrafish embryos.
Neurotoxicology and teratology6963-72(2018 Sep - Oct)
Robinson BL,Dumas M,Ali SF,Paule MG,Gu Q,Kanungo J

A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways.
Nature communications9(1)3090(2018 08)
Piragyte I,Clapes T,Polyzou A,Klein Geltink RI,Lefkopoulos S,Yin N,Cauchy P,Curtis JD,Klaeylé L,Langa X,Beckmann CCA,Wlodarski MW,Müller P,Van Essen D,Rambold A,Kapp FG,Mione M,Buescher JM,Pearce EL,Polyzos A,Trompouki E

Exposure to cocaine and its main metabolites altered the protein profile of zebrafish embryos.
Environmental pollution (Barking, Essex : 1987)232603-614(2018 Jan)
Parolini M,Bini L,Magni S,Rizzo A,Ghilardi A,Landi C,Armini A,Del Giacco L,Binelli A

Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy.
Scientific reports7(1)17417(2017 12)
Nagata Y,Yamagishi M,Konno T,Nakanishi C,Asano Y,Ito S,Nakajima Y,Seguchi O,Fujino N,Kawashiri MA,Takashima S,Kitakaze M,Hayashi K

Altered glycometabolism in zebrafish exposed to thifluzamide.
Chemosphere18389-96(2017 Sep)
Yang Y,Liu W,Li D,Qian L,Fu B,Wang C

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atp5fa1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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