POLE4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLE4
Entrez Gene ID	553610
Full Name	polymerase (DNA-directed), epsilon 4, accessory subunit
Synonyms	zgc:110337
General protein information	Preferred Names polymerase (DNA-directed), epsilon 4, accessory subunit Names DNA polymerase epsilon subunit 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001351798.1	NP_001338727.1	DNA polymerase epsilon subunit 4
XM_017358271.2	XP_017213760.1	DNA polymerase epsilon subunit 4 isoform X1
NM_001020584.1	NP_001018420.1	polymerase (DNA-directed), epsilon 4, accessory subunit
XM_005155711.3	XP_005155768.1	DNA polymerase epsilon subunit 4 isoform X2
XM_017358271.1	XP_017213760.1	DNA polymerase epsilon subunit 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	POLE4	XP_003949865.1
Macaca mulatta (Rhesus monkey)	POLE4	XP_001111739.1
Bos taurus (cattle)	POLE4	NP_001094695.1
Danio rerio (zebrafish)	zgc:110337	XP_005155767.1
Homo sapiens (human)	POLE4	NP_063949.2
Canis lupus familiaris (dog)	POLE4	XP_540212.3
Mus musculus (house mouse)	Pole4	NP_080158.1
Rattus norvegicus (Norway rat)	Pole4	NP_001102104.1
Xenopus tropicalis (tropical clawed frog)	pole4	NP_001016605.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLE4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa16724	NM_001351798.1 Latest version!	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa35669	NM_001020584.1 Latest version!	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa16724	XM_005155711.3 Latest version!	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
ODa54301	XM_017358271.2 Latest version!	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
ODa54301	XM_017358271.1	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLE4 ( NM_001351798.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001338727.1 Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.

Price & Availability↑

CloneID	ODa16724
Clone ID Related Accession (Same CDS sequence)	NM_001351798.1 , XM_005155711.3
Accession Version	NM_001351798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542902400000
Organism	Danio rerio(zebrafish)
Product	DNA polymerase epsilon subunit 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH448501.1 and EH461577.1. On May 31, 2017 this sequence version replaced XM_005155711.3. ##Evidence-Data-START## Transcript exon combination :: EH591945.1, GDQH01004056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476754, SAMEA4476799 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTACATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338727.1
CDS	122..505
Translation	MAATASAAPAESELDRSGAEEEPRGTEPEEDAGSGQTGPTAGAQQHRLARLPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDEFAFLEGTLD

Target ORF information:

RefSeq Version	NM_001351798.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351798.1

ORF Insert Sequence:

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTACATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE4 ( NM_001020584.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001018420.1 Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.

Price & Availability↑

CloneID	ODa35669
Clone ID Related Accession (Same CDS sequence)	NM_001020584.1
Accession Version	NM_001020584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1422028800000
Organism	Danio rerio(Zebrafish)
Product	polymerase (DNA-directed), epsilon 4, accessory subunit
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC095224.1. On or before Oct 5, 2014 this sequence version replaced XM_009305808.1, XM_005155710.1, XM_704656.1, XM_704657.1, XM_678158.1. ##Evidence-Data-START## Transcript exon combination :: BC095224.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTGCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GACAGTTTTA TGGTTCATTC TGATTTCCTC TATTTCGTTA CTCCCTTCAA AGCCTCGGTG 
GTCAGGAATG TCGAACCTAA AAACAAAAGA AATTCTGCCA CGGTTTCAGG TTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018420.1
CDS	89..628
Translation	MAATASAAPAESELDRSGAEEEPRGTEPEEDAGSGQTGPTAGAQQHRLARLPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDEFAFLEGKRFNVISCWQSSKGAGDSFMVHSDFLYFVTPFKASVVRNVEPKNKRNSATVSGYV

Target ORF information:

RefSeq Version	NM_001020584.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020584.1

ORF Insert Sequence:

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTGCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GACAGTTTTA TGGTTCATTC TGATTTCCTC TATTTCGTTA CTCCCTTCAA AGCCTCGGTG 
GTCAGGAATG TCGAACCTAA AAACAAAAGA AATTCTGCCA CGGTTTCAGG TTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE4 ( XM_005155711.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155768.1 Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16724
Clone ID Related Accession (Same CDS sequence)	NM_001351798.1 , XM_005155711.3
Accession Version	XM_005155711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase epsilon subunit 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTACATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155768.1
CDS	164..547
Translation	MAATASAAPAESELDRSGAEEEPRGTEPEEDAGSGQTGPTAGAQQHRLARLPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDEFAFLEGTLD

Target ORF information:

RefSeq Version	XM_005155711.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155711.3

ORF Insert Sequence:

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTACATTGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE4 ( XM_017358271.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213760.1 Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54301
Clone ID Related Accession (Same CDS sequence)	XM_017358271.2 , XM_017358271.1
Accession Version	XM_017358271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	DNA polymerase epsilon subunit 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GAAAGTTTTA TGGTTCATTC TGATTTCCTA TATTTCGTTA CTCCCTTCAA AGCCTCGTTT 
TCCAGGAATG TCGAACTTAA AAACAAAAGA AATTCTGCCA CGGTGTCTGG CTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213760.1
CDS	137..676
Translation	MAATASAAPAESELDRSGAEEEPRGTEPEEDAGSGQTGPTAGAQQHRLARLPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDEFAFLEGKRFNVISCWQSSKGAGESFMVHSDFLYFVTPFKASFSRNVELKNKRNSATVSGYV

Target ORF information:

RefSeq Version	XM_017358271.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358271.2

ORF Insert Sequence:

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GAAAGTTTTA TGGTTCATTC TGATTTCCTA TATTTCGTTA CTCCCTTCAA AGCCTCGTTT 
TCCAGGAATG TCGAACTTAA AAACAAAAGA AATTCTGCCA CGGTGTCTGG CTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE4 ( XM_017358271.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213760.1 Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54301
Clone ID Related Accession (Same CDS sequence)	XM_017358271.2 , XM_017358271.1
Accession Version	XM_017358271.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase epsilon subunit 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GAAAGTTTTA TGGTTCATTC TGATTTCCTA TATTTCGTTA CTCCCTTCAA AGCCTCGTTT 
TCCAGGAATG TCGAACTTAA AAACAAAAGA AATTCTGCCA CGGTGTCTGG CTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213760.1
CDS	165..704
Translation	MAATASAAPAESELDRSGAEEEPRGTEPEEDAGSGQTGPTAGAQQHRLARLPLSRIKTLMKADPDVTLASQESVFIIAKATELFVEMIAKDALVYAQQGKRKTLQRKDLDNAIEAIDEFAFLEGKRFNVISCWQSSKGAGESFMVHSDFLYFVTPFKASFSRNVELKNKRNSATVSGYV

Target ORF information:

RefSeq Version	XM_017358271.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA-directed), epsilon 4, accessory subunit (pole4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358271.1

ORF Insert Sequence:

ATGGCGGCGA CCGCGTCTGC AGCTCCCGCG GAATCGGAGT TGGACCGCAG CGGGGCTGAA 
GAAGAGCCCC GGGGCACCGA GCCGGAGGAA GACGCGGGGA GCGGGCAAAC CGGGCCCACC 
GCGGGCGCAC AGCAACACCG GCTCGCCAGG CTTCCGCTGT CCCGCATTAA AACCCTGATG 
AAAGCAGATC CGGATGTGAC TCTGGCGAGT CAAGAGTCCG TGTTCATCAT AGCTAAAGCT 
ACGGAGCTTT TTGTTGAAAT GATTGCGAAG GACGCTCTCG TTTATGCACA ACAGGGAAAG 
AGAAAAACCC TTCAACGCAA GGACTTGGAC AATGCTATTG AAGCAATAGA CGAATTTGCA 
TTTTTGGAAG GTAAGAGATT TAATGTTATT TCCTGCTGGC AAAGCTCTAA AGGAGCGGGA 
GAAAGTTTTA TGGTTCATTC TGATTTCCTA TATTTCGTTA CTCCCTTCAA AGCCTCGTTT 
TCCAGGAATG TCGAACTTAA AAACAAAAGA AATTCTGCCA CGGTGTCTGG CTATGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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POLE4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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