psmb8f cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	psmb8f
Entrez Gene ID	550488
Full Name	proteasome subunit beta 8f
Synonyms	beta5if,zgc:110346
General protein information	Preferred Names proteasome subunit beta 8f Names proteasome subunit beta 8f proteasome (prosome, macropain) subunit, beta type, 8f proteasome subunit beta type 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017791.1	NP_001017791.1	proteasome 20S subunit beta 8f

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Whole genome duplications have provided teleosts with many roads to peptide loaded MHC class I molecules.
Grimholt U
BMC evolutionary biology18(1)25(2018 02)

Alternative haplotypes of antigen processing genes in zebrafish diverged early in vertebrate evolution.
McConnell SC, Hernandez KM, Wcisel DJ, Kettleborough RN, Stemple DL, Yoder JA, Andrade J, de Jong JL
Proceedings of the National Academy of Sciences of the United States of America113(34)E5014-23(2016 08)

Evolution of dimorphisms of the proteasome subunit beta type 8 gene (PSMB8) in basal ray-finned fish.
Noro M, Nonaka M
Immunogenetics66(5)325-34(2014 May)

Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish.
McConnell SC, Restaino AC, de Jong JL
Immunogenetics66(3)199-213(2014 Mar)

Long-lived dichotomous lineages of the proteasome subunit beta type 8 (PSMB8) gene surviving more than 500 million years as alleles or paralogs.
Tsukamoto K, Miura F, Fujito NT, Yoshizaki G, Nonaka M
Molecular biology and evolution29(10)3071-9(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following psmb8f gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the psmb8f cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01820	NM_001017791.1 Latest version!	Danio rerio proteasome 20S subunit beta 8f (psmb8f), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

psmb8f ( NM_001017791.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017791.1 Danio rerio proteasome 20S subunit beta 8f (psmb8f), mRNA.

Price & Availability↑

CloneID	ODa01820
Clone ID Related Accession (Same CDS sequence)	NM_001017791.1
Accession Version	NM_001017791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Danio rerio(zebrafish)
Product	proteasome 20S subunit beta 8f
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092889.1. ##Evidence-Data-START## Transcript exon combination :: BC092889.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGCTTA TGGACGTATG CTTGTCATCT GAGCTGAAAC AATCATGTTT TACAGCTAAT 
GCTTGGCAAT CTGAGCCGAC CACCTGCGGT CCGAGTCATT ACACGTTCGG ACTAAACACC 
AATGAACTGG CCGTCCCGAC TGGAATGGAC CCGGAGCAGT TTCTCGGGCC ACTCACAGAG 
GGAGATTCGG ACATAAAAAT TGAATTCCTT CATGGCACTA CCACCTTAGC CTTCAAATTT 
CAGCACGGTG TGATGGTGGC TGTGGACTCG AGAGCATCTG CAGGGTCTTA CATAGACACA 
AAAGACTTCA AGAAAGTAAT AGAGATCAAC CCTTATCTCC TTGGCACAAT GTCTGGAAAT 
GCAGCAGACT GTGTTTATTG GGAGAGAAGA CTGGCCAAAG AGTGCAGGAT TTACAAGCTC 
AGAAACAAGA CGCGGATTAG TGTGGCTGCC GCCTCCAAGC TTCTAGCCAA CATGGTGGCT 
GAATATCGTG GCATGGGCCT CTCCATGGGC ACTATGGTTT GTGGCTGGGA CCAGAGAGGT 
CCGGGTCTTT ATTATGTGAG CAGCAGTGGT ACGAGGTTAG CTGGAGATAT GTTCTCCACA 
GGCTCAGGAT GTAACTATGC CTATGGAGTG ATGGACTCAG GGCACAGGTG GGATCTCACC 
GTACCAGAGG CTTATGACTT GGCAGAGAGA GCCATTTATC ATGCTACACA CCGTGATGCC 
TACTCTGGAG GAGTGGTTAA CATGTACCAC ATGCAAAAAG AAGGCTGGAT CAAGGTTTCC 
CAGAATGATG TCGGTGATCT TCATCATAAA TATGTTGCTG AGCAGAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017791.1
CDS	19..852
Translation	MALMDVCLSSELKQSCFTANAWQSEPTTCGPSHYTFGLNTNELAVPTGMDPEQFLGPLTEGDSDIKIEFLHGTTTLAFKFQHGVMVAVDSRASAGSYIDTKDFKKVIEINPYLLGTMSGNAADCVYWERRLAKECRIYKLRNKTRISVAAASKLLANMVAEYRGMGLSMGTMVCGWDQRGPGLYYVSSSGTRLAGDMFSTGSGCNYAYGVMDSGHRWDLTVPEAYDLAERAIYHATHRDAYSGGVVNMYHMQKEGWIKVSQNDVGDLHHKYVAEQKK

Target ORF information:

RefSeq Version	NM_001017791.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proteasome 20S subunit beta 8f (psmb8f), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017791.1

ORF Insert Sequence:

ATGGCGCTTA TGGACGTATG CTTGTCATCT GAGCTGAAAC AATCATGTTT TACAGCTAAT 
GCTTGGCAAT CTGAGCCGAC CACCTGCGGT CCGAGTCATT ACACGTTCGG ACTAAACACC 
AATGAACTGG CCGTCCCGAC TGGAATGGAC CCGGAGCAGT TTCTCGGGCC ACTCACAGAG 
GGAGATTCGG ACATAAAAAT TGAATTCCTT CATGGCACTA CCACCTTAGC CTTCAAATTT 
CAGCACGGTG TGATGGTGGC TGTGGACTCG AGAGCATCTG CAGGGTCTTA CATAGACACA 
AAAGACTTCA AGAAAGTAAT AGAGATCAAC CCTTATCTCC TTGGCACAAT GTCTGGAAAT 
GCAGCAGACT GTGTTTATTG GGAGAGAAGA CTGGCCAAAG AGTGCAGGAT TTACAAGCTC 
AGAAACAAGA CGCGGATTAG TGTGGCTGCC GCCTCCAAGC TTCTAGCCAA CATGGTGGCT 
GAATATCGTG GCATGGGCCT CTCCATGGGC ACTATGGTTT GTGGCTGGGA CCAGAGAGGT 
CCGGGTCTTT ATTATGTGAG CAGCAGTGGT ACGAGGTTAG CTGGAGATAT GTTCTCCACA 
GGCTCAGGAT GTAACTATGC CTATGGAGTG ATGGACTCAG GGCACAGGTG GGATCTCACC 
GTACCAGAGG CTTATGACTT GGCAGAGAGA GCCATTTATC ATGCTACACA CCGTGATGCC 
TACTCTGGAG GAGTGGTTAA CATGTACCAC ATGCAAAAAG AAGGCTGGAT CAAGGTTTCC 
CAGAATGATG TCGGTGATCT TCATCATAAA TATGTTGCTG AGCAGAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole genome duplications have provided teleosts with many roads to peptide loaded MHC class I molecules.
BMC evolutionary biology18(1)25(2018 02)
Grimholt U

Alternative haplotypes of antigen processing genes in zebrafish diverged early in vertebrate evolution.
Proceedings of the National Academy of Sciences of the United States of America113(34)E5014-23(2016 08)
McConnell SC,Hernandez KM,Wcisel DJ,Kettleborough RN,Stemple DL,Yoder JA,Andrade J,de Jong JL

Evolution of dimorphisms of the proteasome subunit beta type 8 gene (PSMB8) in basal ray-finned fish.
Immunogenetics66(5)325-34(2014 May)
Noro M,Nonaka M

Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish.
Immunogenetics66(3)199-213(2014 Mar)
McConnell SC,Restaino AC,de Jong JL

Long-lived dichotomous lineages of the proteasome subunit beta type 8 (PSMB8) gene surviving more than 500 million years as alleles or paralogs.
Molecular biology and evolution29(10)3071-9(2012 Oct)
Tsukamoto K,Miura F,Fujito NT,Yoshizaki G,Nonaka M