gli2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gli2b
Entrez Gene ID	548610
Full Name	GLI family zinc finger 2b
General protein information	Preferred Names GLI family zinc finger 2b Names GLI family zinc finger 2b GLI-Kruppel family member 2b GLI-Kruppel family member GLI2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001015069.1	NP_001015069.1	GLI family zinc finger 2b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.
Hu Z, Chen B, Zhao Q
Biology open8(5)(2019 May)

DNA methylation and gene expression alterations in zebrafish early-life stages exposed to the antibacterial agent triclosan.
Falisse E, Ducos B, Stockwell PA, Morison IM, Chatterjee A, Silvestre F
Environmental pollution (Barking, Essex : 1987)243(Pt B)1867-1877(2018 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling.
Wang J, Cao J, Dickson AL, Poss KD
Nature522(7555)226-230(2015 Jun)

Cis-regulatory control of human GLI2 expression in the developing neural tube and limb bud.
Minhas R, Pauls S, Ali S, Doglio L, Khan MR, Elgar G, Abbasi AA
Developmental dynamics : an official publication of the American Association of Anatomists244(5)681-92(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gli2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gli2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02698	NM_001015069.1 Latest version!	Danio rerio GLI family zinc finger 2b (gli2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gli2b ( NM_001015069.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001015069.1 Danio rerio GLI family zinc finger 2b (gli2b), mRNA.

Price & Availability↑

CloneID	ODa02698
Clone ID Related Accession (Same CDS sequence)	NM_001015069.1
Accession Version	NM_001015069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570204800000
Organism	Danio rerio(zebrafish)
Product	GLI family zinc finger 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY928397.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY928397.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505382, SAMEA3505390 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGCCG GTGTTCCAGC TCCCGCAGAG AAGAAGGACT GTAAATCTTC TCCTCTGGAA 
GGAAACGTCT TCAGCGAAAT GCCCAAAAAA CCTTCTCCTT CATCCCTGAG CAGAGGGCCT 
CATCACATCT TCCCGACTTT CCATTCTCCG ATTCCCATCG ACGTGCGTCA CCATGAGGGG 
AGATACCACT ACGATCCCCA CGCGCTTCAC GCTTTGCACG GTCCTCCAGG TTTAGCGGGC 
AGTCCAGTGA TCTCCGACAT CTCTCTGATC CGTCTGTCTC CGGGAGCCGC CGGCGAGGCT 
TTTCTCCCTC CGCACTCATA CGTCTCTCCG CATGTCGAGC ATTACCTGCG ATCGGCTCAC 
AGCAGCCCAA CGCTGTCCAT GATCTCAGCG GCCCGCGGGC TCAGTCCAGC AGAAGTGGCC 
CACGAGCACC TGAAGGAGCG AGGGTTGTTT GGTCTTCCTC CTCCTCCTGG TGCGTCTCCA 
GCGGATTATT ACCACCTGAT GGCCGGCCAT CGGAACCCGT ATGGAGAACT ACTGATGCAG 
GGAGCTGGAG CCGCAGCGGC CGCCGCTCAC CTGCCAGAAT ACTTCACATC AGTGGACGTG 
TCCCGGTTTC CCAGTCCTCG TATGACGCCC AGGTTGAGCC GTAAGCGAGC GCTGTCCATC 
TCTCCGCTCT CAGACGCCAG CATTGACTTG CAGACCATGA TCCGAACGTC CCCCAACTCT 
CTGGTGGCCT ACATCAACAA CTCACGCTCC AGTTCGGCCG CCAGCAGCTC CTATGGACAC 
CTGTCAGTCG GAGGAATCAG CCCTTCGTTC CCGTTCCCAC ACTCCATTAA TCCAGTGGCG 
TATCAGCAGC TGTTGACCCA GCAGCGAGGC CTCAGTGCGT TCGGACACAC GCCGCCGCTT 
ATTCAGCCAT CACCAACATT CTCCAGCAGA CACCCGCTGA GTCTGTCCAC TCTGCCCACG 
CCAACCACCA ATGACACGGA GACCAAGAAC TCAAATGGAG AGTCTGCGGT CAGCAGCACA 
GTCAACCCAC TCGGCAACAA GAGGTCAAAG GTCAAGACGG AGTTGGGCGG GCCGCGACCT 
GTGTCACCCT ACTCACCGGA GCACCTGAGC AGTGCACAGG ACCTGAAGGA GGACATGGAC 
GACTGTAAGC AGGAGGCGGA GCTTGTGTAC GAGACCAACT GCCACTGGGA GGGCTGCAGC 
AAAGAGTACG ACACGCAGGA GCAGCTGGTG CATCACATCA ACAATGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGGAC GAGTGCTCAC GCGAGCAGAA GCCCTTCAAA 
GCGCAGTACA TGCTGGTGGT CCACATGCGC AGACACACCG GGGAGAAACC GCACAAATGC 
ACGTTCGAGG GCTGCTCAAA GGCGTATTCT CGTCTGGAGA ACCTGAAGAC TCACCTGCGT 
TCACACACTG GAGAGAAACC GTACGTCTGT GAACACGAGG GCTGCAACAA GGCCTTCTCC 
AACGCCTCCG ACCGAGCCAA ACACCAGAAC CGCACACACT CCAACGAGAA ACCCTACGTG 
TGTAAGATCC CGGGCTGTAC CAAGCGCTAT ACTGATCCCA GTTCGCTTCG GAAACACGTT 
AAAACCGTGC ACGGGCCCGA AGCACACGTC ACCAAGAAAC AACGCGGAGA TCTGCCGTCC 
AGACCACATC CGCCGAAGGA GAACGGAGAA AATGAAGCCG GAGCCAAGAT GGCGGAGGAG 
AAAATTGAAG CCAACAGCAC TACTAGAGGA GTGGAGGACT ACCTGCAGGT CAAGTCTATC 
AAGACTGAGA ACTCAATGAT GTATCAGTCC AGCCCTGGTG GTCAGTCATC ATGCAGCAGC 
GAGCCGTCAC CGCTTGGCAG TGCCAGCCTC CATGGCGGTG GAGTAGAGCC GGCGGGACTC 
AGCGGGGGCA GCGTGGGAGA TCTGAGTGCC CTGGATGACG TGCCCATTGT GGATTCCACC 
GTCTCCACAG GAATCCTGGG CCTTCATTTG CGCAAGAACG CCGGCCCGGC CCACAGACTC 
ACACACCTGA AGCAGGAGAA GCTGAAGTCG GTGCGGGACT CGTGCTCCTG GGCAAACACA 
GCTCCTCCTG CCCCCTGCAC CAAACTACCA CCCATCAATA CTGGTTCTTT ATTGGACGGT 
CTGTGTGATC CAGGGCTTTC CATCTCCAGC CCGCGTCTGG GCGACCTCTG TCCTGGCGAG 
ACTACAGTGT TAAGTCAGTT GGTGGAGCGT CGGGACAGCC TGGCCAGCAC TGTGAGCTCT 
GCGTACACCT TCAGCCGGCG CTCATCCGGA ATATCACCAT GTTATTCGAG CCGTCGGTCC 
AGCCAAACAT CCCAACCTGG CGGCCGTCAC AACAACATCA GCTCTGCTGA CTCCTATGAC 
CCCATTTCAA CCGATCTGTC TCGAAGGTCC AGCGAGGCCA GCCTATATGG GGGAATCTTC 
AGCTTGACTC CTGCACAACA CTACCGACTT AAAGCCAAAT ATGCTGCGGC CACAGGCGGA 
GCACCACCCA CGCCTCTGCC TAACATAGAC TGCAAGACTC TGTTAGGAGA TTCGCATGAA 
ATCCCCATAC ATCCCCCTTT GGTTCCCAGA AGGTGCAGTG ACACCGGCTA TGCCAACCGT 
AGCGTTTTAC CCCATGAGGT GACAGCAAAC ATTACTCGAA GAGCAAGCGA CCCAGTTAGA 
CGAATAGCTG CTGAGCGGCA TTCGCTCTAC AACAGTGTAA ACCCGTATCC AACATTACAC 
CCTCTTGCGT CAAGCCGACA CTTTTACAGC ACGTCTGAGA GTAACGTCAA CCGCCAGCAA 
TATCCACCTC GAGCTCCGAG CATTTCTGAG AACGTGATGA TGGAAACTCT GCCGGATGAT 
ATGGAGAGTT GCAATGGTGT TGAGGATGGT TTGATGATGG CTGATGATGC TGTTCAGTGT 
ATCACAACTC AAAACGCAGA TTCCCCTCAA CATACAGGCG TAGCGCACCG GAACCAAAGC 
TTTGGTTCTC CACAACATTG TCATCTCCAG AGACGTCTGG CTTTGGTGGA GGGGAATATG 
AATGCTGTAG CTAGACAAAC AGCCAGCTCT CAGCAGACAT TTCCAGGTTC GGCCAATGCC 
ACAAAGATCC CGATGCCGGT GCAGTGGAAT GAGGTCAGTT CAGGGACGGT GGATTCATCT 
CGCAGCCAAA CCAAGCAGGG ATCCGGGCGG GGAAACCTCC AACAAAAACA AAACCTCAGC 
TCATTTCTGA ATGTCAACTC CAACCAGCAA GTCGGTCTGA TGAGCCACAA CCTTGCCTTG 
AGTCCCGTAC AGCGCAGTGT TGAGTTTAAT TCCCTGAGGA TCAATCAGAG GCAGGCTAAC 
GCAGAGTTTC TGCATGGTTA CCTTCAGGGT TCTGCTTCGA ATGACATCAG TCTTGGTTTG 
CTAAACCCGT CTGGTAAATC GCTGACTGTA CAACCTGGTG GCGTGCAGAA TAATAGGGTA 
TGCGCTCAAG CCCAAAGCCA TGCCACTTTT CCAAATAATC AAGGAAATTA CTCCCAGATG 
AGCAGCAACC AGCAAGGCTA CATCGTAGCG TCCCAGAATG TGAACGGAAA CCTGCTCCTA 
CAGCCAAGGC CGCCCACAGA ACCCAAACCC TACAGCACGC AGCATTCAGG GCCAACAACA 
ACTGCAGCAC AATCCAACTT TAGCCCTGCA TACGACACCT CAGAGGCAAG CCCAAAAAAA 
TCCAGCTCCA TGTTTTACAC CGGCCAGATT CACATGTTTG ATTCGAGCGG CGCTATGCCC 
TGCCCATCTT CAGTGGGCTC CCCTGAAACT AATCAAGTCT CCAGTACGGT GGATTCAAGT 
GGAGCCGATC CCCCGCAGAT TGATTTTGAC GCTATGCTAG ACGACGGCGA TCATTCCAGC 
CTCATGTCAG GAACTTTAAG CCCCGCCCTC CTGCGCAGCC TATCGCAGAA TTCCTCTCGT 
CTCACCACGC CCAGAAATTC GGTGACGTTG CCGCCGGTGG CCGCAGGCAT CAGTAACATG 
GCCATCGGAG ACATGAGCTC CATGTTAACT GCGCTAGCAG AGGAGAGCAA ATTCCTCAAT 
ATGATGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015069.1
CDS	329..4420
Translation	MEAGVPAPAEKKDCKSSPLEGNVFSEMPKKPSPSSLSRGPHHIFPTFHSPIPIDVRHHEGRYHYDPHALHALHGPPGLAGSPVISDISLIRLSPGAAGEAFLPPHSYVSPHVEHYLRSAHSSPTLSMISAARGLSPAEVAHEHLKERGLFGLPPPPGASPADYYHLMAGHRNPYGELLMQGAGAAAAAAHLPEYFTSVDVSRFPSPRMTPRLSRKRALSISPLSDASIDLQTMIRTSPNSLVAYINNSRSSSAASSSYGHLSVGGISPSFPFPHSINPVAYQQLLTQQRGLSAFGHTPPLIQPSPTFSSRHPLSLSTLPTPTTNDTETKNSNGESAVSSTVNPLGNKRSKVKTELGGPRPVSPYSPEHLSSAQDLKEDMDDCKQEAELVYETNCHWEGCSKEYDTQEQLVHHINNEHIHGEKKEFVCRWDECSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDLPSRPHPPKENGENEAGAKMAEEKIEANSTTRGVEDYLQVKSIKTENSMMYQSSPGGQSSCSSEPSPLGSASLHGGGVEPAGLSGGSVGDLSALDDVPIVDSTVSTGILGLHLRKNAGPAHRLTHLKQEKLKSVRDSCSWANTAPPAPCTKLPPINTGSLLDGLCDPGLSISSPRLGDLCPGETTVLSQLVERRDSLASTVSSAYTFSRRSSGISPCYSSRRSSQTSQPGGRHNNISSADSYDPISTDLSRRSSEASLYGGIFSLTPAQHYRLKAKYAAATGGAPPTPLPNIDCKTLLGDSHEIPIHPPLVPRRCSDTGYANRSVLPHEVTANITRRASDPVRRIAAERHSLYNSVNPYPTLHPLASSRHFYSTSESNVNRQQYPPRAPSISENVMMETLPDDMESCNGVEDGLMMADDAVQCITTQNADSPQHTGVAHRNQSFGSPQHCHLQRRLALVEGNMNAVARQTASSQQTFPGSANATKIPMPVQWNEVSSGTVDSSRSQTKQGSGRGNLQQKQNLSSFLNVNSNQQVGLMSHNLALSPVQRSVEFNSLRINQRQANAEFLHGYLQGSASNDISLGLLNPSGKSLTVQPGGVQNNRVCAQAQSHATFPNNQGNYSQMSSNQQGYIVASQNVNGNLLLQPRPPTEPKPYSTQHSGPTTTAAQSNFSPAYDTSEASPKKSSSMFYTGQIHMFDSSGAMPCPSSVGSPETNQVSSTVDSSGADPPQIDFDAMLDDGDHSSLMSGTLSPALLRSLSQNSSRLTTPRNSVTLPPVAAGISNMAIGDMSSMLTALAEESKFLNMMS

Target ORF information:

RefSeq Version	NM_001015069.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLI family zinc finger 2b (gli2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015069.1

ORF Insert Sequence:

ATGGAGGCCG GTGTTCCAGC TCCCGCAGAG AAGAAGGACT GTAAATCTTC TCCTCTGGAA 
GGAAACGTCT TCAGCGAAAT GCCCAAAAAA CCTTCTCCTT CATCCCTGAG CAGAGGGCCT 
CATCACATCT TCCCGACTTT CCATTCTCCG ATTCCCATCG ACGTGCGTCA CCATGAGGGG 
AGATACCACT ACGATCCCCA CGCGCTTCAC GCTTTGCACG GTCCTCCAGG TTTAGCGGGC 
AGTCCAGTGA TCTCCGACAT CTCTCTGATC CGTCTGTCTC CGGGAGCCGC CGGCGAGGCT 
TTTCTCCCTC CGCACTCATA CGTCTCTCCG CATGTCGAGC ATTACCTGCG ATCGGCTCAC 
AGCAGCCCAA CGCTGTCCAT GATCTCAGCG GCCCGCGGGC TCAGTCCAGC AGAAGTGGCC 
CACGAGCACC TGAAGGAGCG AGGGTTGTTT GGTCTTCCTC CTCCTCCTGG TGCGTCTCCA 
GCGGATTATT ACCACCTGAT GGCCGGCCAT CGGAACCCGT ATGGAGAACT ACTGATGCAG 
GGAGCTGGAG CCGCAGCGGC CGCCGCTCAC CTGCCAGAAT ACTTCACATC AGTGGACGTG 
TCCCGGTTTC CCAGTCCTCG TATGACGCCC AGGTTGAGCC GTAAGCGAGC GCTGTCCATC 
TCTCCGCTCT CAGACGCCAG CATTGACTTG CAGACCATGA TCCGAACGTC CCCCAACTCT 
CTGGTGGCCT ACATCAACAA CTCACGCTCC AGTTCGGCCG CCAGCAGCTC CTATGGACAC 
CTGTCAGTCG GAGGAATCAG CCCTTCGTTC CCGTTCCCAC ACTCCATTAA TCCAGTGGCG 
TATCAGCAGC TGTTGACCCA GCAGCGAGGC CTCAGTGCGT TCGGACACAC GCCGCCGCTT 
ATTCAGCCAT CACCAACATT CTCCAGCAGA CACCCGCTGA GTCTGTCCAC TCTGCCCACG 
CCAACCACCA ATGACACGGA GACCAAGAAC TCAAATGGAG AGTCTGCGGT CAGCAGCACA 
GTCAACCCAC TCGGCAACAA GAGGTCAAAG GTCAAGACGG AGTTGGGCGG GCCGCGACCT 
GTGTCACCCT ACTCACCGGA GCACCTGAGC AGTGCACAGG ACCTGAAGGA GGACATGGAC 
GACTGTAAGC AGGAGGCGGA GCTTGTGTAC GAGACCAACT GCCACTGGGA GGGCTGCAGC 
AAAGAGTACG ACACGCAGGA GCAGCTGGTG CATCACATCA ACAATGAGCA CATCCACGGG 
GAGAAGAAGG AGTTTGTGTG CCGCTGGGAC GAGTGCTCAC GCGAGCAGAA GCCCTTCAAA 
GCGCAGTACA TGCTGGTGGT CCACATGCGC AGACACACCG GGGAGAAACC GCACAAATGC 
ACGTTCGAGG GCTGCTCAAA GGCGTATTCT CGTCTGGAGA ACCTGAAGAC TCACCTGCGT 
TCACACACTG GAGAGAAACC GTACGTCTGT GAACACGAGG GCTGCAACAA GGCCTTCTCC 
AACGCCTCCG ACCGAGCCAA ACACCAGAAC CGCACACACT CCAACGAGAA ACCCTACGTG 
TGTAAGATCC CGGGCTGTAC CAAGCGCTAT ACTGATCCCA GTTCGCTTCG GAAACACGTT 
AAAACCGTGC ACGGGCCCGA AGCACACGTC ACCAAGAAAC AACGCGGAGA TCTGCCGTCC 
AGACCACATC CGCCGAAGGA GAACGGAGAA AATGAAGCCG GAGCCAAGAT GGCGGAGGAG 
AAAATTGAAG CCAACAGCAC TACTAGAGGA GTGGAGGACT ACCTGCAGGT CAAGTCTATC 
AAGACTGAGA ACTCAATGAT GTATCAGTCC AGCCCTGGTG GTCAGTCATC ATGCAGCAGC 
GAGCCGTCAC CGCTTGGCAG TGCCAGCCTC CATGGCGGTG GAGTAGAGCC GGCGGGACTC 
AGCGGGGGCA GCGTGGGAGA TCTGAGTGCC CTGGATGACG TGCCCATTGT GGATTCCACC 
GTCTCCACAG GAATCCTGGG CCTTCATTTG CGCAAGAACG CCGGCCCGGC CCACAGACTC 
ACACACCTGA AGCAGGAGAA GCTGAAGTCG GTGCGGGACT CGTGCTCCTG GGCAAACACA 
GCTCCTCCTG CCCCCTGCAC CAAACTACCA CCCATCAATA CTGGTTCTTT ATTGGACGGT 
CTGTGTGATC CAGGGCTTTC CATCTCCAGC CCGCGTCTGG GCGACCTCTG TCCTGGCGAG 
ACTACAGTGT TAAGTCAGTT GGTGGAGCGT CGGGACAGCC TGGCCAGCAC TGTGAGCTCT 
GCGTACACCT TCAGCCGGCG CTCATCCGGA ATATCACCAT GTTATTCGAG CCGTCGGTCC 
AGCCAAACAT CCCAACCTGG CGGCCGTCAC AACAACATCA GCTCTGCTGA CTCCTATGAC 
CCCATTTCAA CCGATCTGTC TCGAAGGTCC AGCGAGGCCA GCCTATATGG GGGAATCTTC 
AGCTTGACTC CTGCACAACA CTACCGACTT AAAGCCAAAT ATGCTGCGGC CACAGGCGGA 
GCACCACCCA CGCCTCTGCC TAACATAGAC TGCAAGACTC TGTTAGGAGA TTCGCATGAA 
ATCCCCATAC ATCCCCCTTT GGTTCCCAGA AGGTGCAGTG ACACCGGCTA TGCCAACCGT 
AGCGTTTTAC CCCATGAGGT GACAGCAAAC ATTACTCGAA GAGCAAGCGA CCCAGTTAGA 
CGAATAGCTG CTGAGCGGCA TTCGCTCTAC AACAGTGTAA ACCCGTATCC AACATTACAC 
CCTCTTGCGT CAAGCCGACA CTTTTACAGC ACGTCTGAGA GTAACGTCAA CCGCCAGCAA 
TATCCACCTC GAGCTCCGAG CATTTCTGAG AACGTGATGA TGGAAACTCT GCCGGATGAT 
ATGGAGAGTT GCAATGGTGT TGAGGATGGT TTGATGATGG CTGATGATGC TGTTCAGTGT 
ATCACAACTC AAAACGCAGA TTCCCCTCAA CATACAGGCG TAGCGCACCG GAACCAAAGC 
TTTGGTTCTC CACAACATTG TCATCTCCAG AGACGTCTGG CTTTGGTGGA GGGGAATATG 
AATGCTGTAG CTAGACAAAC AGCCAGCTCT CAGCAGACAT TTCCAGGTTC GGCCAATGCC 
ACAAAGATCC CGATGCCGGT GCAGTGGAAT GAGGTCAGTT CAGGGACGGT GGATTCATCT 
CGCAGCCAAA CCAAGCAGGG ATCCGGGCGG GGAAACCTCC AACAAAAACA AAACCTCAGC 
TCATTTCTGA ATGTCAACTC CAACCAGCAA GTCGGTCTGA TGAGCCACAA CCTTGCCTTG 
AGTCCCGTAC AGCGCAGTGT TGAGTTTAAT TCCCTGAGGA TCAATCAGAG GCAGGCTAAC 
GCAGAGTTTC TGCATGGTTA CCTTCAGGGT TCTGCTTCGA ATGACATCAG TCTTGGTTTG 
CTAAACCCGT CTGGTAAATC GCTGACTGTA CAACCTGGTG GCGTGCAGAA TAATAGGGTA 
TGCGCTCAAG CCCAAAGCCA TGCCACTTTT CCAAATAATC AAGGAAATTA CTCCCAGATG 
AGCAGCAACC AGCAAGGCTA CATCGTAGCG TCCCAGAATG TGAACGGAAA CCTGCTCCTA 
CAGCCAAGGC CGCCCACAGA ACCCAAACCC TACAGCACGC AGCATTCAGG GCCAACAACA 
ACTGCAGCAC AATCCAACTT TAGCCCTGCA TACGACACCT CAGAGGCAAG CCCAAAAAAA 
TCCAGCTCCA TGTTTTACAC CGGCCAGATT CACATGTTTG ATTCGAGCGG CGCTATGCCC 
TGCCCATCTT CAGTGGGCTC CCCTGAAACT AATCAAGTCT CCAGTACGGT GGATTCAAGT 
GGAGCCGATC CCCCGCAGAT TGATTTTGAC GCTATGCTAG ACGACGGCGA TCATTCCAGC 
CTCATGTCAG GAACTTTAAG CCCCGCCCTC CTGCGCAGCC TATCGCAGAA TTCCTCTCGT 
CTCACCACGC CCAGAAATTC GGTGACGTTG CCGCCGGTGG CCGCAGGCAT CAGTAACATG 
GCCATCGGAG ACATGAGCTC CATGTTAACT GCGCTAGCAG AGGAGAGCAA ATTCCTCAAT 
ATGATGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.
Biology open8(5)(2019 May)
Hu Z,Chen B,Zhao Q

DNA methylation and gene expression alterations in zebrafish early-life stages exposed to the antibacterial agent triclosan.
Environmental pollution (Barking, Essex : 1987)243(Pt B)1867-1877(2018 Dec)
Falisse E,Ducos B,Stockwell PA,Morison IM,Chatterjee A,Silvestre F

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling.
Nature522(7555)226-230(2015 Jun)
Wang J,Cao J,Dickson AL,Poss KD

Cis-regulatory control of human GLI2 expression in the developing neural tube and limb bud.
Developmental dynamics : an official publication of the American Association of Anatomists244(5)681-92(2015 May)
Minhas R,Pauls S,Ali S,Doglio L,Khan MR,Elgar G,Abbasi AA

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gli2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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