galca cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	galca
Entrez Gene ID	449649
Full Name	galactosylceramidase a
Synonyms	galc,galcl,si:ch211-199l3.4,zgc:92561
General protein information	Preferred Names galactosylceramidase a Names galactocerebrosidase GALCERase galactosylceramidase like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005921.1	NP_001005921.1	galactocerebrosidase precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Molecular cloning and knockdown of galactocerebrosidase in zebrafish: new insights into the pathogenesis of Krabbe's disease.
Zizioli D, Guarienti M, Tobia C, Gariano G, Borsani G, Bresciani R, Ronca R, Giacopuzzi E, Preti A, Gaudenzi G, Belleri M, Di Salle E, Fabrias G, Casas J, Ribatti D, Monti E, Presta M
Biochimica et biophysica acta1842(4)665-75(2014 Apr)

Early life stage trimethyltin exposure induces ADP-ribosylation factor expression and perturbs the vascular system in zebrafish.
Chen J, Huang C, Truong L, La Du J, Tilton SC, Waters KM, Lin K, Tanguay RL, Dong Q
Toxicology302(2-3)129-39(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following galca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the galca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01394	NM_001005921.1 Latest version!	Danio rerio galactosylceramidase a (galca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

galca ( NM_001005921.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001005921.1 Danio rerio galactosylceramidase a (galca), mRNA.

Price & Availability↑

CloneID	ODa01394
Clone ID Related Accession (Same CDS sequence)	NM_001005921.1
Accession Version	NM_001005921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Danio rerio(zebrafish)
Product	galactocerebrosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083517.1. ##Evidence-Data-START## Transcript exon combination :: BC083517.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAAACGC ACAATTTTCT GTGCATAATC TTCGTCATTC TCGGATGTTC GGCTCAGTTT 
TATGTTATAG ATGATCGGAT TGGTTTAGGG CGGGAGTTTG ATGGAATAGG AGGCTTAAGT 
GGTGGAGGAG CCACTTCTCG ACTTCTTGTC AATTATGAAG AACCCTATCG GAGTCAGATA 
TTGGATTACT TATTCAAGCC CAACTTTGGA GCTTCACTTC ACATCCTCAA GGTAGAAATA 
GGAGGCGATG CTCAAACCAC AGATGGCACA GAACCCTCTC ACATGCACAG TAAGGATGAG 
GGGAATTTTT TCCGTGGATA CGAGTGGTGG CTTATGAAGG AAGCCAAGAA AAGAAATCCA 
AACATTAAGC TGATCGGTCT TCCCTGGGCA TTTCCCGGAT GGGTTGGATA CGGAACCCAA 
TGGCCTTACT TTTTTCCAAA TGTTACAGCC AACTATGTTA TCACATGGGT CATGGGAGCA 
AAGCAGCATC ATAACCTTGA CATTGATTAT ATCGGGATTT GGAATGAGAA AGCATTTGAT 
CCGACATACA TTAAGGTGCT GAGAGATGTT TTGGACAGAG CTGGCTTCAC GAATATCGGC 
ATCATTGCTG CAGATGGAGA CTGGTCTATT GCGTCTGCCA TGCTGGATGA TCCATATCTG 
AATGATGCTG TCGAGGTCAT AGGTGTCCAC TACCCAGGAA CCAACACAGT AAAGGAAGCT 
CTGTTGACGG AGAGAAAGCT CTGGTCATCT GAAGACTACA GCACCTACAA TGACGACATC 
GGTGCCGGAT GTTGGGCTCG CATTCTCAAC CAGAACTACG TGAATGGCAA AATGACCTCG 
ACTATATCCT GGAATGTAAT AGCAAGTTAT TATGAGAATC TGTCATTTGG TAGAGATGGT 
CTGATGACGG CAGAGGAGCC CTGGAGTGGA CATTATATAG TGGAATCTCC AATATGGATG 
ACTGCTCACA CTACTCAATT CACACAGCCA GGCTGGTTTT ATCTGCAGAC TGTGGGCAAA 
CTCAACCACG GAGGAAGTTA TGTGGCTTTG ACAGACCGCA AAGGGAACTT AACAATTATT 
ATAGAAACCA TGACACACGA GCACTCTCAG TGCATTCGTC CACCATTGCC GCACTTTGAC 
GTCTCTCCTC AGATCGCCAC ATTTGAGCTC AAAGGCTCCT TTGCACATCT CGCTGACCTT 
CAAGTGTGGT ACTCAAAACT GGATTTTAAG TCTGGAAATG GTACACTGTT TAGGCAACTG 
AGGCCTATTA GGGCCCACAA TGGCTTGTTA AGCTTAAAAC TAGATGTCGA TGAGGTCTTT 
ACTATCACAA CGGTCACCAC TGCGCAAAGA GGCTTCTACC CAGAGCCCCC CAAATCATGC 
CCGTTCCCTA AGAATTACAC CGATGATTTT ACAATAGATA ACCCTCCATT CTCTGAGGCC 
CCGTACTTTG CTGATCAAAC CGGTGTGTTT GAGTACTTCA GAAACACTAC AGATAACAGC 
TCCCATGCCT TTACTCTTCG CCAAGTGGTC ATTGAGCGGC CGGTGGCCTG GGCTAAAGAT 
GCCGACCAAA CCATCAGCAT CATAGGAGAC TACAGCTGGT CAGACGTAAA TGTGTCATGT 
GATGTCTTTA TTGAAACCCC CAAGACTGGA GGAGTGTTCC TGGCGGCCAG GGTCGATCAA 
GGCGGAGAAT CAGTCCGACA AGCAAAGGGG GTCTTTTACT GGATTTATGC AAATGGGACC 
TATAGAGTGA CAAATGACAT AGGTGGGAAA GGAGTTCTTG CTGAAGGGCT GTCAGGAACA 
AAGGCAGGAG TATGGTACAC CTTAACTCTT AGCGTCAAGG GATATTTTGC CACAGGGTCA 
CTGAACGGCT TTCCGTTGTG GAAAAATGCT GCTGTTCTGG AGCCCAAGAG TGGTTGGGCT 
GCACTAGGAA CTTTATCTTT TGAATACGCA CAGTTTGATA ATTTTAATGT GATTGCAGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005921.1
CDS	72..2054
Translation	MQTHNFLCIIFVILGCSAQFYVIDDRIGLGREFDGIGGLSGGGATSRLLVNYEEPYRSQILDYLFKPNFGASLHILKVEIGGDAQTTDGTEPSHMHSKDEGNFFRGYEWWLMKEAKKRNPNIKLIGLPWAFPGWVGYGTQWPYFFPNVTANYVITWVMGAKQHHNLDIDYIGIWNEKAFDPTYIKVLRDVLDRAGFTNIGIIAADGDWSIASAMLDDPYLNDAVEVIGVHYPGTNTVKEALLTERKLWSSEDYSTYNDDIGAGCWARILNQNYVNGKMTSTISWNVIASYYENLSFGRDGLMTAEEPWSGHYIVESPIWMTAHTTQFTQPGWFYLQTVGKLNHGGSYVALTDRKGNLTIIIETMTHEHSQCIRPPLPHFDVSPQIATFELKGSFAHLADLQVWYSKLDFKSGNGTLFRQLRPIRAHNGLLSLKLDVDEVFTITTVTTAQRGFYPEPPKSCPFPKNYTDDFTIDNPPFSEAPYFADQTGVFEYFRNTTDNSSHAFTLRQVVIERPVAWAKDADQTISIIGDYSWSDVNVSCDVFIETPKTGGVFLAARVDQGGESVRQAKGVFYWIYANGTYRVTNDIGGKGVLAEGLSGTKAGVWYTLTLSVKGYFATGSLNGFPLWKNAAVLEPKSGWAALGTLSFEYAQFDNFNVIAG

Target ORF information:

RefSeq Version	NM_001005921.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio galactosylceramidase a (galca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005921.1

ORF Insert Sequence:

ATGCAAACGC ACAATTTTCT GTGCATAATC TTCGTCATTC TCGGATGTTC GGCTCAGTTT 
TATGTTATAG ATGATCGGAT TGGTTTAGGG CGGGAGTTTG ATGGAATAGG AGGCTTAAGT 
GGTGGAGGAG CCACTTCTCG ACTTCTTGTC AATTATGAAG AACCCTATCG GAGTCAGATA 
TTGGATTACT TATTCAAGCC CAACTTTGGA GCTTCACTTC ACATCCTCAA GGTAGAAATA 
GGAGGCGATG CTCAAACCAC AGATGGCACA GAACCCTCTC ACATGCACAG TAAGGATGAG 
GGGAATTTTT TCCGTGGATA CGAGTGGTGG CTTATGAAGG AAGCCAAGAA AAGAAATCCA 
AACATTAAGC TGATCGGTCT TCCCTGGGCA TTTCCCGGAT GGGTTGGATA CGGAACCCAA 
TGGCCTTACT TTTTTCCAAA TGTTACAGCC AACTATGTTA TCACATGGGT CATGGGAGCA 
AAGCAGCATC ATAACCTTGA CATTGATTAT ATCGGGATTT GGAATGAGAA AGCATTTGAT 
CCGACATACA TTAAGGTGCT GAGAGATGTT TTGGACAGAG CTGGCTTCAC GAATATCGGC 
ATCATTGCTG CAGATGGAGA CTGGTCTATT GCGTCTGCCA TGCTGGATGA TCCATATCTG 
AATGATGCTG TCGAGGTCAT AGGTGTCCAC TACCCAGGAA CCAACACAGT AAAGGAAGCT 
CTGTTGACGG AGAGAAAGCT CTGGTCATCT GAAGACTACA GCACCTACAA TGACGACATC 
GGTGCCGGAT GTTGGGCTCG CATTCTCAAC CAGAACTACG TGAATGGCAA AATGACCTCG 
ACTATATCCT GGAATGTAAT AGCAAGTTAT TATGAGAATC TGTCATTTGG TAGAGATGGT 
CTGATGACGG CAGAGGAGCC CTGGAGTGGA CATTATATAG TGGAATCTCC AATATGGATG 
ACTGCTCACA CTACTCAATT CACACAGCCA GGCTGGTTTT ATCTGCAGAC TGTGGGCAAA 
CTCAACCACG GAGGAAGTTA TGTGGCTTTG ACAGACCGCA AAGGGAACTT AACAATTATT 
ATAGAAACCA TGACACACGA GCACTCTCAG TGCATTCGTC CACCATTGCC GCACTTTGAC 
GTCTCTCCTC AGATCGCCAC ATTTGAGCTC AAAGGCTCCT TTGCACATCT CGCTGACCTT 
CAAGTGTGGT ACTCAAAACT GGATTTTAAG TCTGGAAATG GTACACTGTT TAGGCAACTG 
AGGCCTATTA GGGCCCACAA TGGCTTGTTA AGCTTAAAAC TAGATGTCGA TGAGGTCTTT 
ACTATCACAA CGGTCACCAC TGCGCAAAGA GGCTTCTACC CAGAGCCCCC CAAATCATGC 
CCGTTCCCTA AGAATTACAC CGATGATTTT ACAATAGATA ACCCTCCATT CTCTGAGGCC 
CCGTACTTTG CTGATCAAAC CGGTGTGTTT GAGTACTTCA GAAACACTAC AGATAACAGC 
TCCCATGCCT TTACTCTTCG CCAAGTGGTC ATTGAGCGGC CGGTGGCCTG GGCTAAAGAT 
GCCGACCAAA CCATCAGCAT CATAGGAGAC TACAGCTGGT CAGACGTAAA TGTGTCATGT 
GATGTCTTTA TTGAAACCCC CAAGACTGGA GGAGTGTTCC TGGCGGCCAG GGTCGATCAA 
GGCGGAGAAT CAGTCCGACA AGCAAAGGGG GTCTTTTACT GGATTTATGC AAATGGGACC 
TATAGAGTGA CAAATGACAT AGGTGGGAAA GGAGTTCTTG CTGAAGGGCT GTCAGGAACA 
AAGGCAGGAG TATGGTACAC CTTAACTCTT AGCGTCAAGG GATATTTTGC CACAGGGTCA 
CTGAACGGCT TTCCGTTGTG GAAAAATGCT GCTGTTCTGG AGCCCAAGAG TGGTTGGGCT 
GCACTAGGAA CTTTATCTTT TGAATACGCA CAGTTTGATA ATTTTAATGT GATTGCAGGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Molecular cloning and knockdown of galactocerebrosidase in zebrafish: new insights into the pathogenesis of Krabbe's disease.
Biochimica et biophysica acta1842(4)665-75(2014 Apr)
Zizioli D,Guarienti M,Tobia C,Gariano G,Borsani G,Bresciani R,Ronca R,Giacopuzzi E,Preti A,Gaudenzi G,Belleri M,Di Salle E,Fabrias G,Casas J,Ribatti D,Monti E,Presta M

Early life stage trimethyltin exposure induces ADP-ribosylation factor expression and perturbs the vascular system in zebrafish.
Toxicology302(2-3)129-39(2012 Dec)
Chen J,Huang C,Truong L,La Du J,Tilton SC,Waters KM,Lin K,Tanguay RL,Dong Q

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galca cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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