acsl4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	acsl4a
Entrez Gene ID	393622
Full Name	acyl-CoA synthetase long chain family member 4a
Synonyms	acsl4,zgc:66186
General protein information	Preferred Names acyl-CoA synthetase long chain family member 4a Names long-chain-fatty-acid--CoA ligase 4 acsl4b acyl-CoA synthetase long-chain family member 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200649.1	NP_956943.1	long-chain-fatty-acid--CoA ligase 4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acsl4	NP_956943.1
Caenorhabditis elegans (roundworm)	acs-4	NP_498568.1
Canis lupus familiaris (dog)	ACSL4	XP_005641723.1
Caenorhabditis elegans (roundworm)	acs-17	NP_508993.2
Homo sapiens (human)	ACSL4	NP_075266.1
Rattus norvegicus (Norway rat)	Acsl4	NP_446075.1
Xenopus tropicalis (tropical clawed frog)	LOC100494759	XP_004916909.1
Oryza sativa (rice)	Os12g0168700	NP_001066256.1
Pan troglodytes (chimpanzee)	ACSL4	XP_003317677.1
Mus musculus (house mouse)	Acsl4	NP_997508.1
Gallus gallus (chicken)	ACSL4	XP_004940863.1
Arabidopsis thaliana (thale cress)	LACS9	NP_177882.1
Macaca mulatta (Rhesus monkey)	ACSL4	XP_001099465.1
Bos taurus (cattle)	ACSL4	XP_005227959.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Lipid Uptake, Metabolism, and Transport in the Larval Zebrafish.
Quinlivan VH, Farber SA
Frontiers in endocrinology8319(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
Ellis LD, Soo EC, Achenbach JC, Morash MG, Soanes KH
PloS one9(12)e115305(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acsl4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acsl4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01410	NM_200649.1 Latest version!	Danio rerio acyl-CoA synthetase long chain family member 4a (acsl4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acsl4a ( NM_200649.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956943.1 Danio rerio acyl-CoA synthetase long chain family member 4a (acsl4a), mRNA.

Price & Availability↑

CloneID	ODa01410
Clone ID Related Accession (Same CDS sequence)	NM_200649.1
Accession Version	NM_200649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548432000000
Organism	Danio rerio(zebrafish)
Product	long-chain-fatty-acid--CoA ligase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057476.1. ##Evidence-Data-START## Transcript exon combination :: BC057476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACCTCA GTGCCGTTCT CTTGTTTCCA GTACATGCAG TAGTGTGGCT CTACTCCCTC 
TTGAGCTTTC TGCCATGGTA CTTTCTGACA GGAGCCCAGG AAAAGAAAGC CTTGGCAAAG 
AGGCTCAAAT CCAAGTCAAC CAGCGATCAA CCGGATGGAC CATATCGCTC CACGGATCGC 
TTCGAGTCCC TGGTCACAGT GGACTTCCCT GGAAAAGACA CCCTTGATAA GCTGTTTGAC 
TATGCAGTAG AGCGTTTTGG TAAATTAGAC TGCCTTGGAA CCCGAGAAGT TCTGAGTGGA 
GACAATGAGA CTCAGCCAAA TGGAAAAGTC TTCAAAAAGT TGATTCTTGG TGAATATAAA 
TGGAAGTCCT ATGAGGAGTT AGACATTGAA GTCAGTCATT TTGGGAGCGG ACTGGCAGCA 
CTAGGACAAC AGCCAAAAAG CACCATTGCC ATTTTCTGTG AGACGCGGGC TGAATGGATG 
ATCACCGCTC AAGCCTGCTT CAGACGCAAC TTCCCCTTGG TCACCTTTTA TGCGACTCTT 
GGAGAAGACG CAGTGGCTTA TGGATTGAAT GAGTCTGGAG TTACACACCT GGTCACCAGT 
ATAGAATTGC TGGAAACAAA ACTAAAGAGT GTTCTTCTTC AAATTCCCAA TTTAAAGCAC 
ATCATCTATG TGGACAGTAA GAGCATGAAC ACAGCAGGAT TTCCACAGGG CATCCAGATC 
CACAGCATGC ACTCAGTTGA AGAGCTGGGA GCCAAGCCAG AAAACTTGAA TAGACCAATA 
GTGAAGCCCG TCCCATCAGA TCTTGCTGTT ATTATGTACA CCAGCGGCTC CACCGGGAGA 
CCTAAAGGGG TGATGATCAT CCACAGTAAC CTGATTGGAG GCATGGCAGG CCAGTGTGAG 
AGGATACCTG GGTTGGGACC GAAGGATACA TATATTGCTT ATCTTCCTCT TGCTCATGTG 
CTGGAAATGA CGGCTGAGAT CTCATGTGTG ACATACGGCT GCAGAATCGG CTATTCTTCA 
CCACAAACCC TGTCTGATCA ATCAACCAAA ATTAAAAAGG GAAGTAAAGG GGACTGCAGC 
GTACTGAAAC CTTCCCTTAT GGCCGCTGTA CCTGAAATAA TGGACCGCAT CAATAAAAAC 
GTGATGAGTA AAGTGCAAGA GATGAGCTGT GTGCAGAGAA CCTTGTTCAA GCCGGCCTAC 
GACTATAAGC TCGAGCAGAT CAAGAAGGGT TACGATGCGC CGCTCTGCAA TATCTTGTTT 
AAGAAAGTCC GAGCTCTGCT GGGTGGAAAT GTGCGAATGA TGTTGTCTGG AGGAGCTCCT 
CTGTCTCCTG CTACTCAGCG CTTCATGAAC ATCTGTTTCT GCTGTCCTGT TGGTCAGGGA 
TATGGTTTAA CGGAGACCTG TGGAGCAGGC ACTATCACCG AAGTTGCAGA CTACAGCACA 
GGACGAGTAG GAGCTCCTCT GATCTGCTGT GAGGTCAAAC TCAAAGACTG GGCAGAAGGT 
GGTTATACAA AGCGTGACAA GCCTCATCCG CGTGGCGAGA TCCTCATCGG TGGCCCTAAT 
GTGACTATGG GCTACTACAG GACTGAAGGC TGTAATAATG ACGACTTCTT TGTCGATCAG 
AATGGCCAGC GGTGGTTCTG CACTGGCGAT ATAGGAGAGA TGCATGCTGA CGGCTGTCTA 
CAGATTGTTG ACCGCAAGAA GGATCTTGTA AAACTTCAAG CTGGAGAGTA CGTCTCTTTG 
GGCAAAGTTG AGTCAGCGCT GAAGAACTGC TCTTTCGTTG ACAACATCTG CGCTTACGCA 
AACAGTGACC AGAACTACGT TATCAGCTTC ATTGTTCCTA ATCAGAAGAA ACTGACTGCT 
CTTGCCAATC AGAAAGGCAT TGAAGGCACG TGGGAAGAAA TCTGTAACCA CCCAGTTATG 
GAAAGGGAAG TTCTGAGAGA AATTAAAGAA GTCGCAAATT CAATTAAACT CCAGCGCTTC 
GAGATTCCTG TCATGGTCCA TTTGAGTCCG GAGCCGTGGA CACCTGAAAC CGGCCTGGTG 
ACAGATGCAT TCAAACTGAA GAGGAAAGAG CTAAAGAACC ACTATCTCAA CGACATCGAA 
AGAATGTATG GCCACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956943.1
CDS	211..2331
Translation	MDLSAVLLFPVHAVVWLYSLLSFLPWYFLTGAQEKKALAKRLKSKSTSDQPDGPYRSTDRFESLVTVDFPGKDTLDKLFDYAVERFGKLDCLGTREVLSGDNETQPNGKVFKKLILGEYKWKSYEELDIEVSHFGSGLAALGQQPKSTIAIFCETRAEWMITAQACFRRNFPLVTFYATLGEDAVAYGLNESGVTHLVTSIELLETKLKSVLLQIPNLKHIIYVDSKSMNTAGFPQGIQIHSMHSVEELGAKPENLNRPIVKPVPSDLAVIMYTSGSTGRPKGVMIIHSNLIGGMAGQCERIPGLGPKDTYIAYLPLAHVLEMTAEISCVTYGCRIGYSSPQTLSDQSTKIKKGSKGDCSVLKPSLMAAVPEIMDRINKNVMSKVQEMSCVQRTLFKPAYDYKLEQIKKGYDAPLCNILFKKVRALLGGNVRMMLSGGAPLSPATQRFMNICFCCPVGQGYGLTETCGAGTITEVADYSTGRVGAPLICCEVKLKDWAEGGYTKRDKPHPRGEILIGGPNVTMGYYRTEGCNNDDFFVDQNGQRWFCTGDIGEMHADGCLQIVDRKKDLVKLQAGEYVSLGKVESALKNCSFVDNICAYANSDQNYVISFIVPNQKKLTALANQKGIEGTWEEICNHPVMEREVLREIKEVANSIKLQRFEIPVMVHLSPEPWTPETGLVTDAFKLKRKELKNHYLNDIERMYGHK

Target ORF information:

RefSeq Version	NM_200649.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acyl-CoA synthetase long chain family member 4a (acsl4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200649.1

ORF Insert Sequence:

ATGGACCTCA GTGCCGTTCT CTTGTTTCCA GTACATGCAG TAGTGTGGCT CTACTCCCTC 
TTGAGCTTTC TGCCATGGTA CTTTCTGACA GGAGCCCAGG AAAAGAAAGC CTTGGCAAAG 
AGGCTCAAAT CCAAGTCAAC CAGCGATCAA CCGGATGGAC CATATCGCTC CACGGATCGC 
TTCGAGTCCC TGGTCACAGT GGACTTCCCT GGAAAAGACA CCCTTGATAA GCTGTTTGAC 
TATGCAGTAG AGCGTTTTGG TAAATTAGAC TGCCTTGGAA CCCGAGAAGT TCTGAGTGGA 
GACAATGAGA CTCAGCCAAA TGGAAAAGTC TTCAAAAAGT TGATTCTTGG TGAATATAAA 
TGGAAGTCCT ATGAGGAGTT AGACATTGAA GTCAGTCATT TTGGGAGCGG ACTGGCAGCA 
CTAGGACAAC AGCCAAAAAG CACCATTGCC ATTTTCTGTG AGACGCGGGC TGAATGGATG 
ATCACCGCTC AAGCCTGCTT CAGACGCAAC TTCCCCTTGG TCACCTTTTA TGCGACTCTT 
GGAGAAGACG CAGTGGCTTA TGGATTGAAT GAGTCTGGAG TTACACACCT GGTCACCAGT 
ATAGAATTGC TGGAAACAAA ACTAAAGAGT GTTCTTCTTC AAATTCCCAA TTTAAAGCAC 
ATCATCTATG TGGACAGTAA GAGCATGAAC ACAGCAGGAT TTCCACAGGG CATCCAGATC 
CACAGCATGC ACTCAGTTGA AGAGCTGGGA GCCAAGCCAG AAAACTTGAA TAGACCAATA 
GTGAAGCCCG TCCCATCAGA TCTTGCTGTT ATTATGTACA CCAGCGGCTC CACCGGGAGA 
CCTAAAGGGG TGATGATCAT CCACAGTAAC CTGATTGGAG GCATGGCAGG CCAGTGTGAG 
AGGATACCTG GGTTGGGACC GAAGGATACA TATATTGCTT ATCTTCCTCT TGCTCATGTG 
CTGGAAATGA CGGCTGAGAT CTCATGTGTG ACATACGGCT GCAGAATCGG CTATTCTTCA 
CCACAAACCC TGTCTGATCA ATCAACCAAA ATTAAAAAGG GAAGTAAAGG GGACTGCAGC 
GTACTGAAAC CTTCCCTTAT GGCCGCTGTA CCTGAAATAA TGGACCGCAT CAATAAAAAC 
GTGATGAGTA AAGTGCAAGA GATGAGCTGT GTGCAGAGAA CCTTGTTCAA GCCGGCCTAC 
GACTATAAGC TCGAGCAGAT CAAGAAGGGT TACGATGCGC CGCTCTGCAA TATCTTGTTT 
AAGAAAGTCC GAGCTCTGCT GGGTGGAAAT GTGCGAATGA TGTTGTCTGG AGGAGCTCCT 
CTGTCTCCTG CTACTCAGCG CTTCATGAAC ATCTGTTTCT GCTGTCCTGT TGGTCAGGGA 
TATGGTTTAA CGGAGACCTG TGGAGCAGGC ACTATCACCG AAGTTGCAGA CTACAGCACA 
GGACGAGTAG GAGCTCCTCT GATCTGCTGT GAGGTCAAAC TCAAAGACTG GGCAGAAGGT 
GGTTATACAA AGCGTGACAA GCCTCATCCG CGTGGCGAGA TCCTCATCGG TGGCCCTAAT 
GTGACTATGG GCTACTACAG GACTGAAGGC TGTAATAATG ACGACTTCTT TGTCGATCAG 
AATGGCCAGC GGTGGTTCTG CACTGGCGAT ATAGGAGAGA TGCATGCTGA CGGCTGTCTA 
CAGATTGTTG ACCGCAAGAA GGATCTTGTA AAACTTCAAG CTGGAGAGTA CGTCTCTTTG 
GGCAAAGTTG AGTCAGCGCT GAAGAACTGC TCTTTCGTTG ACAACATCTG CGCTTACGCA 
AACAGTGACC AGAACTACGT TATCAGCTTC ATTGTTCCTA ATCAGAAGAA ACTGACTGCT 
CTTGCCAATC AGAAAGGCAT TGAAGGCACG TGGGAAGAAA TCTGTAACCA CCCAGTTATG 
GAAAGGGAAG TTCTGAGAGA AATTAAAGAA GTCGCAAATT CAATTAAACT CCAGCGCTTC 
GAGATTCCTG TCATGGTCCA TTTGAGTCCG GAGCCGTGGA CACCTGAAAC CGGCCTGGTG 
ACAGATGCAT TCAAACTGAA GAGGAAAGAG CTAAAGAACC ACTATCTCAA CGACATCGAA 
AGAATGTATG GCCACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Lipid Uptake, Metabolism, and Transport in the Larval Zebrafish.
Frontiers in endocrinology8319(2017)
Quinlivan VH,Farber SA

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity.
PloS one9(12)e115305(2014)
Ellis LD,Soo EC,Achenbach JC,Morash MG,Soanes KH

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acsl4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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