hspa4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hspa4b
Entrez Gene ID	334086
Full Name	heat shock protein 4b
Synonyms	hspa4,wu:fi30e11,zgc:55743,zgc:77413
General protein information	Preferred Names heat shock protein 4b Names heat shock protein 4b heat shock protein 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199857.1	NP_956151.1	heat shock protein 4b

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SSE1	NP_015219.1
Arabidopsis thaliana (thale cress)	AT1G79920	NP_850984.4
Gallus gallus (chicken)	HSPA4	XP_414655.2
Caenorhabditis elegans (roundworm)	C30C11.4	NP_498868.1
Magnaporthe oryzae (rice blast fungus)	MGG_06065	XP_003711919.1
Eremothecium gossypii	AGOS_AGR212W	NP_986878.1
Oryza sativa (rice)	Os05g0181000	NP_001054812.1
Kluyveromyces lactis	KLLA0E24597g	XP_455059.1
Homo sapiens (human)	HSPA4	NP_002145.3
Mus musculus (house mouse)	Hspa4	NP_032326.3
Danio rerio (zebrafish)	hspa4a	NP_999881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010331	XP_315285.3
Rattus norvegicus (Norway rat)	Hspa4	NP_705893.1
Macaca mulatta (Rhesus monkey)	HSPA4	XP_001106968.1
Danio rerio (zebrafish)	hspa4b	NP_956151.1
Drosophila melanogaster (fruit fly)	Hsc70Cb	NP_001189103.1
Xenopus tropicalis (tropical clawed frog)	hspa4	NP_989252.1
Schizosaccharomyces pombe (fission yeast)	pss1	XP_001713060.1
Pan troglodytes (chimpanzee)	HSPA4	XP_517930.2
Canis lupus familiaris (dog)	HSPA4	NP_001041481.1
Bos taurus (cattle)	HSPA4	NP_001107664.1
Arabidopsis thaliana (thale cress)	HSP91	NP_178111.1
Oryza sativa (rice)	Os01g0180800	NP_001042210.1
Neurospora crassa	NCU05269	XP_961759.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress.
Xu K, Xu H, Han Z
Genes9(12)(2018 Nov)

Fate and effects of silver nanoparticles on early life-stage development of zebrafish (Danio rerio) in comparison to silver nitrate.
Cambier S, Røgeberg M, Georgantzopoulou A, Serchi T, Karlsson C, Verhaegen S, Iversen TG, Guignard C, Kruszewski M, Hoffmann L, Audinot JN, Ropstad E, Gutleb AC
The Science of the total environment610-611972-982(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
Yin X, Wang H, Zhang Y, Dahlgren RA, Zhang H, Shi M, Gao M, Wang X
PloS one9(7)e102731(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hspa4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hspa4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01003	NM_199857.1 Latest version!	Danio rerio heat shock protein 4b (hspa4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hspa4b ( NM_199857.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956151.1 Danio rerio heat shock protein 4b (hspa4b), mRNA.

Price & Availability↑

CloneID	ODa01003
Clone ID Related Accession (Same CDS sequence)	NM_199857.1
Accession Version	NM_199857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555862400000
Organism	Danio rerio(zebrafish)
Product	heat shock protein 4b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC048063.1. On Jul 6, 2005 this sequence version replaced XM_697053.1. ##Evidence-Data-START## Transcript exon combination :: BC048063.1, BC065970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGTGG TGGGTTTTGA CGTGGGTTTT CTCAACTGCT ATGTTGCGGT AGCTCGAGCC 
GGAGGAATCG AGACTGTGGC CAATGAATAC AGCGACCGAT GCACACCAGC ATGTGTGTCT 
TTTGGACCAC GAAATCGGTC TATTGGAGCT GCAGCAAAAA GCCAGATGGT CACCAACTGC 
AAAAACACTG TCCATGGATT CAAGCGTTTT CATGGTCGGG CTTTCTCAGA TCCATTCGTC 
CAGAACTTGA AGCCTAGTCT CGTTTATGAT CTGGCACAGA TGCCATCGGG CACGACGGGC 
TTAAAGGTGA TGTACATGGA GGAGGAGAAG GTGTTCAGCA TTGAACAAGT CACTGCGATG 
CTCCTCACTA AACTCAAGGA GACTGCTGAG AGCGCCCTCA AGAAGCCTGT GGCTGACTGT 
GTCATCTCTG TCCCCTGCTT CTACACCGAT GCTGAAAGGA GATCCGTCAT CGATGCAGCT 
CAGATTGCAG GACTCAACTG TCTGAGACTC ATGAACGAGA CCACTGCAGT GGCTCTGGCA 
TATGGCATCT ACAAGCAGGA TCTACCTGCT CCTGAGGAAA AGCCAAGGAA TGTGGTGTTT 
GTGGATATTG GCCATTCAGG CTACCAGGTT TCTGTGTGTG CCTTTAATAA AGGCAAGCTT 
AAGATTCTTG CTACAGCCTT TGACCCTGAA ATGGGAGGAA AATACTTCGA TGAGAGGCTG 
GTCAAATATT TTTGTGAGGA GTTTGTCGTG AAGTACAAAC TAGATGCGAA GACCAAACCC 
AGAGCTCTGA TCCGGCTCTT CCAGGAATGT GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCGACC TGCCGCTCAA CATCGAGTGT TTCATGAATG ACGTTGATGT CTCAAGCAGA 
CTTAACAGGG CTCAGTTTGA AGAGATGTGT GCTGATATTT TAGCCCGGGT GGAGCCTCCA 
TTGCGCAGCC TCCTGGAACA AGCTCATCTG AAGAAAGATG ACATCCATGC AGTAGAAATA 
GTGGGCGGTG CTTCTAGAAT GCCGGCCATC AAAGAGCGAA TCAGCAAGTT CTTTGGGAAA 
GAGCCGAGCA CAACGCTGAA TGCAGATGAA GCTGTGGCCA GAGGATGTGC TCTCCAGTGT 
GCAATCCTCT CTCCGGCTTT TAAAGTCCGT GAGTTCTCGA TAACAGAAGT GGTTCCGTTT 
CCCATCTCTC TTAAATGGAA TTCTGCAGCT GAAGATGGAG TCAGTGACTG TGAGGTTTTC 
CCAAAGAACC ATGCAGCACC TTTCTCTAAA GTGTTGACCT TTTACCGGAG AGAGCCATTT 
ACCTTGGATG CATACTACAA CTCCCCTAAA GAGCTCCCTT ATCCAGATCC CACAATAGGC 
CAGTATGTTG TCCAGAAAGT GGTTCCCCAG GCCTCCGGTG AGAGTTCAAA GGTTAAAGTG 
AAGGTGCGAG TGAACATTCA TGGCATCTTT AGCGTTTCCA GTGCTTCACT AGTTGAGGTG 
CAGAAATCTG AGGAGGAAGA GGAGAGCATG GAAACCGAGC AGAGCACAGA AAAAGAAAAT 
GAGAACAAAA TGCAGGTTGA TCAAGAGGAA CAAAAGACTC CAGAGACAGA ACAGGAAAAT 
GGCGAGAAAA AACCTGGAAC TGAGGAAATG GAGACCTCAG CAGAGGAAGG CAAACAGGAG 
AAGAAATCAG ATCAGCCTCC CCAAGCCAAG AAGCCCAAGG TGAAGACAAA GGTGCTGGAT 
TTGCCCATTG AAAACAACCC GCAGTGGCAG CTGGCCAACG ACATGCTGAA TCTGTTCGTG 
GAGAGTGAGG GTAAAATGAT CATGCAGGAC AAGCTGGAGA AGGAGCGCAA TGATGCCAAG 
AATTATGTTG AGGAGTATGT GTACGAGATG AGGGACAAAC TGCATGGCAT ATTCGAGAAG 
TTCGTCACTG AGAGTGACAG AGATGTTCTC TCATTAAAAC TAGAAGACAC TGAGGTCTGG 
CTGTATGAGG ATGGAGAAGA CCAGCCCAAA CAAATATACA TCGACAAACT TGCTGAACTG 
AAGAACCTTG GCCAACCCAT TCAAGACAGA TACAGAGAGT TTGAGGAGAG ACCCAAAGCT 
TTTGAAGAGC TGGGCAGACA GTTGCAGCAA TACATGAAGA TTGTTGAGGC CTACAAAACC 
AAGGAAGAGC AGTATGATCA TCTGGAGGAG GCAGAAATTC AGAAGGTGGA CAAGATGGTG 
AATGATGTCA TGATCTGGAT GAACAGTAAA ATGAACCAGC AGAGTAAACA GAGTCTTGCC 
ATCGAGCCAG TGGTGAAAAC GACAGAGATC CAGGCCAAAA CACGGGAGCT GTTTTCTACT 
TGCAATCCAG TCGTGACCAA ACCCAAGCCC AAAGTGGACT TGCCAAAAGA AGAGAACCCA 
TCAGAACCCA ACGGGCCCGT CAACACACAG GAAAACCCTG AAGCTCAGCC CGGCGGCACA 
GAACCAGCTG CTGCAGACAG TGCAGGAAAC ACAGAAAACA AGCCTGACAT GGACCTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956151.1
CDS	117..2639
Translation	MSVVGFDVGFLNCYVAVARAGGIETVANEYSDRCTPACVSFGPRNRSIGAAAKSQMVTNCKNTVHGFKRFHGRAFSDPFVQNLKPSLVYDLAQMPSGTTGLKVMYMEEEKVFSIEQVTAMLLTKLKETAESALKKPVADCVISVPCFYTDAERRSVIDAAQIAGLNCLRLMNETTAVALAYGIYKQDLPAPEEKPRNVVFVDIGHSGYQVSVCAFNKGKLKILATAFDPEMGGKYFDERLVKYFCEEFVVKYKLDAKTKPRALIRLFQECEKLKKLMSANSSDLPLNIECFMNDVDVSSRLNRAQFEEMCADILARVEPPLRSLLEQAHLKKDDIHAVEIVGGASRMPAIKERISKFFGKEPSTTLNADEAVARGCALQCAILSPAFKVREFSITEVVPFPISLKWNSAAEDGVSDCEVFPKNHAAPFSKVLTFYRREPFTLDAYYNSPKELPYPDPTIGQYVVQKVVPQASGESSKVKVKVRVNIHGIFSVSSASLVEVQKSEEEEESMETEQSTEKENENKMQVDQEEQKTPETEQENGEKKPGTEEMETSAEEGKQEKKSDQPPQAKKPKVKTKVLDLPIENNPQWQLANDMLNLFVESEGKMIMQDKLEKERNDAKNYVEEYVYEMRDKLHGIFEKFVTESDRDVLSLKLEDTEVWLYEDGEDQPKQIYIDKLAELKNLGQPIQDRYREFEERPKAFEELGRQLQQYMKIVEAYKTKEEQYDHLEEAEIQKVDKMVNDVMIWMNSKMNQQSKQSLAIEPVVKTTEIQAKTRELFSTCNPVVTKPKPKVDLPKEENPSEPNGPVNTQENPEAQPGGTEPAAADSAGNTENKPDMDLD

Target ORF information:

RefSeq Version	NM_199857.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio heat shock protein 4b (hspa4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199857.1

ORF Insert Sequence:

ATGTCGGTGG TGGGTTTTGA CGTGGGTTTT CTCAACTGCT ATGTTGCGGT AGCTCGAGCC 
GGAGGAATCG AGACTGTGGC CAATGAATAC AGCGACCGAT GCACACCAGC ATGTGTGTCT 
TTTGGACCAC GAAATCGGTC TATTGGAGCT GCAGCAAAAA GCCAGATGGT CACCAACTGC 
AAAAACACTG TCCATGGATT CAAGCGTTTT CATGGTCGGG CTTTCTCAGA TCCATTCGTC 
CAGAACTTGA AGCCTAGTCT CGTTTATGAT CTGGCACAGA TGCCATCGGG CACGACGGGC 
TTAAAGGTGA TGTACATGGA GGAGGAGAAG GTGTTCAGCA TTGAACAAGT CACTGCGATG 
CTCCTCACTA AACTCAAGGA GACTGCTGAG AGCGCCCTCA AGAAGCCTGT GGCTGACTGT 
GTCATCTCTG TCCCCTGCTT CTACACCGAT GCTGAAAGGA GATCCGTCAT CGATGCAGCT 
CAGATTGCAG GACTCAACTG TCTGAGACTC ATGAACGAGA CCACTGCAGT GGCTCTGGCA 
TATGGCATCT ACAAGCAGGA TCTACCTGCT CCTGAGGAAA AGCCAAGGAA TGTGGTGTTT 
GTGGATATTG GCCATTCAGG CTACCAGGTT TCTGTGTGTG CCTTTAATAA AGGCAAGCTT 
AAGATTCTTG CTACAGCCTT TGACCCTGAA ATGGGAGGAA AATACTTCGA TGAGAGGCTG 
GTCAAATATT TTTGTGAGGA GTTTGTCGTG AAGTACAAAC TAGATGCGAA GACCAAACCC 
AGAGCTCTGA TCCGGCTCTT CCAGGAATGT GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCGACC TGCCGCTCAA CATCGAGTGT TTCATGAATG ACGTTGATGT CTCAAGCAGA 
CTTAACAGGG CTCAGTTTGA AGAGATGTGT GCTGATATTT TAGCCCGGGT GGAGCCTCCA 
TTGCGCAGCC TCCTGGAACA AGCTCATCTG AAGAAAGATG ACATCCATGC AGTAGAAATA 
GTGGGCGGTG CTTCTAGAAT GCCGGCCATC AAAGAGCGAA TCAGCAAGTT CTTTGGGAAA 
GAGCCGAGCA CAACGCTGAA TGCAGATGAA GCTGTGGCCA GAGGATGTGC TCTCCAGTGT 
GCAATCCTCT CTCCGGCTTT TAAAGTCCGT GAGTTCTCGA TAACAGAAGT GGTTCCGTTT 
CCCATCTCTC TTAAATGGAA TTCTGCAGCT GAAGATGGAG TCAGTGACTG TGAGGTTTTC 
CCAAAGAACC ATGCAGCACC TTTCTCTAAA GTGTTGACCT TTTACCGGAG AGAGCCATTT 
ACCTTGGATG CATACTACAA CTCCCCTAAA GAGCTCCCTT ATCCAGATCC CACAATAGGC 
CAGTATGTTG TCCAGAAAGT GGTTCCCCAG GCCTCCGGTG AGAGTTCAAA GGTTAAAGTG 
AAGGTGCGAG TGAACATTCA TGGCATCTTT AGCGTTTCCA GTGCTTCACT AGTTGAGGTG 
CAGAAATCTG AGGAGGAAGA GGAGAGCATG GAAACCGAGC AGAGCACAGA AAAAGAAAAT 
GAGAACAAAA TGCAGGTTGA TCAAGAGGAA CAAAAGACTC CAGAGACAGA ACAGGAAAAT 
GGCGAGAAAA AACCTGGAAC TGAGGAAATG GAGACCTCAG CAGAGGAAGG CAAACAGGAG 
AAGAAATCAG ATCAGCCTCC CCAAGCCAAG AAGCCCAAGG TGAAGACAAA GGTGCTGGAT 
TTGCCCATTG AAAACAACCC GCAGTGGCAG CTGGCCAACG ACATGCTGAA TCTGTTCGTG 
GAGAGTGAGG GTAAAATGAT CATGCAGGAC AAGCTGGAGA AGGAGCGCAA TGATGCCAAG 
AATTATGTTG AGGAGTATGT GTACGAGATG AGGGACAAAC TGCATGGCAT ATTCGAGAAG 
TTCGTCACTG AGAGTGACAG AGATGTTCTC TCATTAAAAC TAGAAGACAC TGAGGTCTGG 
CTGTATGAGG ATGGAGAAGA CCAGCCCAAA CAAATATACA TCGACAAACT TGCTGAACTG 
AAGAACCTTG GCCAACCCAT TCAAGACAGA TACAGAGAGT TTGAGGAGAG ACCCAAAGCT 
TTTGAAGAGC TGGGCAGACA GTTGCAGCAA TACATGAAGA TTGTTGAGGC CTACAAAACC 
AAGGAAGAGC AGTATGATCA TCTGGAGGAG GCAGAAATTC AGAAGGTGGA CAAGATGGTG 
AATGATGTCA TGATCTGGAT GAACAGTAAA ATGAACCAGC AGAGTAAACA GAGTCTTGCC 
ATCGAGCCAG TGGTGAAAAC GACAGAGATC CAGGCCAAAA CACGGGAGCT GTTTTCTACT 
TGCAATCCAG TCGTGACCAA ACCCAAGCCC AAAGTGGACT TGCCAAAAGA AGAGAACCCA 
TCAGAACCCA ACGGGCCCGT CAACACACAG GAAAACCCTG AAGCTCAGCC CGGCGGCACA 
GAACCAGCTG CTGCAGACAG TGCAGGAAAC ACAGAAAACA AGCCTGACAT GGACCTTGAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress.
Genes9(12)(2018 Nov)
Xu K,Xu H,Han Z

Fate and effects of silver nanoparticles on early life-stage development of zebrafish (Danio rerio) in comparison to silver nitrate.
The Science of the total environment610-611972-982(2018 Jan)
Cambier S,Røgeberg M,Georgantzopoulou A,Serchi T,Karlsson C,Verhaegen S,Iversen TG,Guignard C,Kruszewski M,Hoffmann L,Audinot JN,Ropstad E,Gutleb AC

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis.
PloS one9(7)e102731(2014)
Yin X,Wang H,Zhang Y,Dahlgren RA,Zhang H,Shi M,Gao M,Wang X

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hspa4b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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