gucy2d cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gucy2d
Entrez Gene ID	140426
Full Name	guanylate cyclase 2D, retinal
Synonyms	GUCY2d1,RETGC-3,gc3
General protein information	Preferred Names guanylate cyclase 2D, retinal Names guanylyl cyclase 3 zat zatoichi
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475989.1	XP_021331664.1	guanylyl cyclase 3 isoform X1
XM_005165262.2	XP_005165319.1	guanylyl cyclase 3 isoform X2
XM_005165261.3	XP_005165318.1	guanylyl cyclase 3 isoform X1
XM_005165261.2	XP_005165318.1	guanylyl cyclase 3 isoform X1
NM_131866.1	NP_571941.1	retinal guanylyl cyclase 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gucy2e	NP_077356.1
Xenopus tropicalis (tropical clawed frog)	gucy2d	XP_002942678.2
Macaca mulatta (Rhesus monkey)	GUCY2D	XP_001111670.1
Danio rerio (zebrafish)	gc3	NP_571941.1
Bos taurus (cattle)	GUCY2D	NP_776973.2
Homo sapiens (human)	GUCY2D	NP_000171.1
Pan troglodytes (chimpanzee)	GUCY2D	XP_003315414.1
Canis lupus familiaris (dog)	GUCY2D	NP_001003207.1
Mus musculus (house mouse)	Gucy2e	NP_032218.2

Gene Ontology
Bibliography

Related articles in PubMed

Transcripts within rod photoreceptors of the Zebrafish retina.
Sun C, Galicia C, Stenkamp DL
BMC genomics19(1)127(2018 02)

Aipl1 is required for cone photoreceptor function and survival through the stability of Pde6c and Gc3 in zebrafish.
Iribarne M, Nishiwaki Y, Nakamura S, Araragi M, Oguri E, Masai I
Scientific reports745962(2017 04)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array.
Tsai HY, Hamilton A, Tinch AE, Guy DR, Gharbi K, Stear MJ, Matika O, Bishop SC, Houston RD
BMC genomics16969(2015 Nov)

Molecular and neuronal homology between the olfactory systems of zebrafish and mouse.
Saraiva LR, Ahuja G, Ivandic I, Syed AS, Marioni JC, Korsching SI, Logan DW
Scientific reports511487(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gucy2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gucy2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02391	NM_131866.1 Latest version!	Danio rerio guanylate cyclase 2D, retinal (gucy2d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa02391	XM_005165262.2 Latest version!	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa10659	XM_021475989.1 Latest version!	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa10659	XM_005165261.3 Latest version!	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa10659	XM_005165261.2	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gucy2d ( NM_131866.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571941.1 Danio rerio guanylate cyclase 2D, retinal (gucy2d), mRNA.

Price & Availability↑

CloneID	ODa02391
Clone ID Related Accession (Same CDS sequence)	XM_005165262.2 , NM_131866.1
Accession Version	NM_131866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573833600000
Organism	Danio rerio(zebrafish)
Product	retinal guanylyl cyclase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC163620.1, EB933544.1 and BC142906.1. On Feb 4, 2011 this sequence version replaced XM_690001.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTTTCTCCA TTTTTTGGGA CCATATATAA CATGATCTAT 
TATACAGCCA TGGCTGCAGA GCAAAGCCGA GCTTCGAGTG GTGGGCGATG GGTATCGGGC 
GATTTACTGT CTCAAAACGA GGGTGGATTT GAATTTGATG GCTTTAACCA GCGGATATCG 
GGAGGTACTA ACGGAGAGGG AATGCAGGTC CGCTATGCTG TCCTGGATTC AGACGGCTCT 
GGGTCCAGCC TGTATCGCAC ACACGCCCTG GAAGCTTCAC ACACATATGG AAAATATGGA 
GGGTTAAAAT ACTTGGGGAA GTCAATTCAT TTTGCTGGAG CGTCTCCGAG CAGTGACGCG 
AACTGCTGGT TTAGTCCTTA TATCGCATGT AGTGGAGGAT TGGATGGAGT GACGCTTTTC 
TTCTTGCTCA TGTTTTTCAT AGTGTTGATA GCGGGTGCAG GCATCCTTTT TCTACAAGTT 
AGAAAATATG GGGCTGGAGG TTTTTTTGGT GCGTTTATTG GAGGAGGAGG AAGCCCCACT 
AAGATAGTGC TGACGCTGGA TGACTTGGTC TTCATCAACA CCACACTCAG CAGAAGGAAA 
CTGAATGACG AGAGTATGCT GAAGAGTCAG CTAGATGTTA AAACCCCGCG CCACTCAGTC 
TCTGGTCGCA GTTACATCAC ATCAACCCCA GAAACATCCA ATGTTGCTGT ATTTGAGGGT 
GACTGGGTCT GGTTAAAGAA ATGCCCCTGT GCAGATTCTA TTACAGAGAT CAGTGAAAGC 
ACACAGACCA TCTTTGTCAA GCTGAGAGAT ATGCGTCATG AGAATCTGAA CTTGTTCCTG 
GGTCTGTTTA TGGATACTGG TATATTCGGC ATTGTTACAG AGCACTGCAC CAGAGGCAGT 
CTGGAAGACC TGCTCAACAA TGAGGAAATG AGGCTTGACT GGATGTTCAA ATCCTCTCTT 
CTCCTCGACC TGATTAGGGG AATGAAGTAC TTGCATAATC GGGGCATTAT TCATGGACGC 
CTGAAGTCTC GGAACTGTGT GGTGGATGGT CGATTTGTTT TGAAGGTTAC AGATTATGGC 
TTCAATGAAA TCCTCAACTG CCAAAACATC ATCCTGGAGG ACAACGCACC AGAGGATCAG 
TTCTGGACAG CTCCGGAGAT CCTGAGGAAT CCTGATCTGA AGAAGAAGGG AACATATCCA 
GGAGATGTAT ACAGCTTTTC TATCATCATG CAGGAGGTGA TCTCTCGCTG CGCACCCTTC 
TGCATGTTGG ATATGCCTCC TGAAGAAATC ATAGAAAAGG TGCGCTCTCC TCCACCACTG 
TGCCGACCCA CTGTGTCTAT GGATGAAGCT CCGCTAGATG TGATTCAGCT CATGAAGCAG 
GCCTGGAGTG AGGAGCCTGA ACAGAGGCCT ACGTTTGAGG ATATATTCAG ACAGTTTAAG 
AGCATGAACA AAGGAAAGAA GACTAACATA ATAGACTCAA TGTTGAGGAT GCTGGAGCAG 
TACTCCTCAA ACCTGGAGGA TCTGATCCGA GAGAGGACCG AAGAACTGGA GGTAGAGCGA 
CAGAAAACTG ATGCTCTTCT AGCCCAGATG CTGCCCAAGT CGGTGGCCTT GGCATTAAAA 
ACAGGGAAGC CAGTGAAGCC AGAGCATTTC GCTGAAGTCA CACTGTATTT CAGTGATATC 
GTGGGTTTCA CCACCATCTC TGCCCTCAGT GAACCCATCG AAGTGGTCGA TCTGCTTAAT 
GACCTCTACA CACACTTTGA TGGAATCATC GCTATCCACG ATGTCTATAA GGTGGAGACT 
ATTGGTGATG CGTACATGGT GGCATCAGGA GTTCCCAATC GCAATGGTAC CCGCCACGCT 
GCAGAGATGG CCAACATGTC CCTGGACATT CTCCACTGCA TCGGTACATT TAAAATGAGG 
CACATGCCTG ACGTCAAAGT CAAAATTCGC ATTGGACTAC ATTCTGGTCC AGTGGTCGCA 
GGTGTAGTTG GCTTAAAGAT GCCTCGATAC TGTCTGTTCG GGGACACAGT AAACACAGCC 
TCCCGAATGG AATCCACAGG GTTGCCTTAC AGAATTCATG TCAACCAGAG TACAGTTGAT 
GTCCTAAACA GTCTCAAACT TGGCTACAAA ATAGATACTC GGGGCAGGAC CGAGCTCAAG 
GGTAAAGGTG TAGAAGAAAC ATTTTGGTTG GTTGGGAGGG ATGGATTTGA CAAGCCGTTA 
CCTATACCGC CAGACCTTAC TCCTGGGGCG AGCAACCATG GTATCAGCTT GGATGAGATC 
CCGACTGATA GGCGACAGAA ATTCTTGGAT CGACAGAAGA GGATGGGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571941.1
CDS	53..3466
Translation	MNDHTSFLCQSHHPRWQTQSSAGKRRRKQSSSQKSETPASPVSSSPVLRSHSWWGNCFLLPLVMLSYFPCEAWGTTFKIAIVGPWACDPMYSKALPDLAARLAMSRINKDPELNKGYWYDYTVINEDCSSSRGLARFAELEGYGSAFLGPANPGYCTAATLLAKNWNKGILSWGCLKAEMDEEMYPTFLRPLPLSSRVLFSVLRFFRWAHVGIVTAEGDMWEATGHELAASLRALGLPVGKVVTMEQDKEGPRQALQAIRDTDRVRVVIMCMHSALIGGEEQYQLLTTALEMRMIDRGYIFIPYDTLLYSLPYENVKYPILANDSKLRRAYDGVLTVTMDQGEQNFYEAFNAAQESFEIRGSTSAEEVSPFFGTIYNMIYYTAMAAEQSRASSGGRWVSGDLLSQNEGGFEFDGFNQRISGGTNGEGMQVRYAVLDSDGSGSSLYRTHALEASHTYGKYGGLKYLGKSIHFAGASPSSDANCWFSPYIACSGGLDGVTLFFLLMFFIVLIAGAGILFLQVRKYGAGGFFGAFIGGGGSPTKIVLTLDDLVFINTTLSRRKLNDESMLKSQLDVKTPRHSVSGRSYITSTPETSNVAVFEGDWVWLKKCPCADSITEISESTQTIFVKLRDMRHENLNLFLGLFMDTGIFGIVTEHCTRGSLEDLLNNEEMRLDWMFKSSLLLDLIRGMKYLHNRGIIHGRLKSRNCVVDGRFVLKVTDYGFNEILNCQNIILEDNAPEDQFWTAPEILRNPDLKKKGTYPGDVYSFSIIMQEVISRCAPFCMLDMPPEEIIEKVRSPPPLCRPTVSMDEAPLDVIQLMKQAWSEEPEQRPTFEDIFRQFKSMNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEVERQKTDALLAQMLPKSVALALKTGKPVKPEHFAEVTLYFSDIVGFTTISALSEPIEVVDLLNDLYTHFDGIIAIHDVYKVETIGDAYMVASGVPNRNGTRHAAEMANMSLDILHCIGTFKMRHMPDVKVKIRIGLHSGPVVAGVVGLKMPRYCLFGDTVNTASRMESTGLPYRIHVNQSTVDVLNSLKLGYKIDTRGRTELKGKGVEETFWLVGRDGFDKPLPIPPDLTPGASNHGISLDEIPTDRRQKFLDRQKRMGN

Target ORF information:

RefSeq Version	NM_131866.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylate cyclase 2D, retinal (gucy2d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131866.1

ORF Insert Sequence:

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTTTCTCCA TTTTTTGGGA CCATATATAA CATGATCTAT 
TATACAGCCA TGGCTGCAGA GCAAAGCCGA GCTTCGAGTG GTGGGCGATG GGTATCGGGC 
GATTTACTGT CTCAAAACGA GGGTGGATTT GAATTTGATG GCTTTAACCA GCGGATATCG 
GGAGGTACTA ACGGAGAGGG AATGCAGGTC CGCTATGCTG TCCTGGATTC AGACGGCTCT 
GGGTCCAGCC TGTATCGCAC ACACGCCCTG GAAGCTTCAC ACACATATGG AAAATATGGA 
GGGTTAAAAT ACTTGGGGAA GTCAATTCAT TTTGCTGGAG CGTCTCCGAG CAGTGACGCG 
AACTGCTGGT TTAGTCCTTA TATCGCATGT AGTGGAGGAT TGGATGGAGT GACGCTTTTC 
TTCTTGCTCA TGTTTTTCAT AGTGTTGATA GCGGGTGCAG GCATCCTTTT TCTACAAGTT 
AGAAAATATG GGGCTGGAGG TTTTTTTGGT GCGTTTATTG GAGGAGGAGG AAGCCCCACT 
AAGATAGTGC TGACGCTGGA TGACTTGGTC TTCATCAACA CCACACTCAG CAGAAGGAAA 
CTGAATGACG AGAGTATGCT GAAGAGTCAG CTAGATGTTA AAACCCCGCG CCACTCAGTC 
TCTGGTCGCA GTTACATCAC ATCAACCCCA GAAACATCCA ATGTTGCTGT ATTTGAGGGT 
GACTGGGTCT GGTTAAAGAA ATGCCCCTGT GCAGATTCTA TTACAGAGAT CAGTGAAAGC 
ACACAGACCA TCTTTGTCAA GCTGAGAGAT ATGCGTCATG AGAATCTGAA CTTGTTCCTG 
GGTCTGTTTA TGGATACTGG TATATTCGGC ATTGTTACAG AGCACTGCAC CAGAGGCAGT 
CTGGAAGACC TGCTCAACAA TGAGGAAATG AGGCTTGACT GGATGTTCAA ATCCTCTCTT 
CTCCTCGACC TGATTAGGGG AATGAAGTAC TTGCATAATC GGGGCATTAT TCATGGACGC 
CTGAAGTCTC GGAACTGTGT GGTGGATGGT CGATTTGTTT TGAAGGTTAC AGATTATGGC 
TTCAATGAAA TCCTCAACTG CCAAAACATC ATCCTGGAGG ACAACGCACC AGAGGATCAG 
TTCTGGACAG CTCCGGAGAT CCTGAGGAAT CCTGATCTGA AGAAGAAGGG AACATATCCA 
GGAGATGTAT ACAGCTTTTC TATCATCATG CAGGAGGTGA TCTCTCGCTG CGCACCCTTC 
TGCATGTTGG ATATGCCTCC TGAAGAAATC ATAGAAAAGG TGCGCTCTCC TCCACCACTG 
TGCCGACCCA CTGTGTCTAT GGATGAAGCT CCGCTAGATG TGATTCAGCT CATGAAGCAG 
GCCTGGAGTG AGGAGCCTGA ACAGAGGCCT ACGTTTGAGG ATATATTCAG ACAGTTTAAG 
AGCATGAACA AAGGAAAGAA GACTAACATA ATAGACTCAA TGTTGAGGAT GCTGGAGCAG 
TACTCCTCAA ACCTGGAGGA TCTGATCCGA GAGAGGACCG AAGAACTGGA GGTAGAGCGA 
CAGAAAACTG ATGCTCTTCT AGCCCAGATG CTGCCCAAGT CGGTGGCCTT GGCATTAAAA 
ACAGGGAAGC CAGTGAAGCC AGAGCATTTC GCTGAAGTCA CACTGTATTT CAGTGATATC 
GTGGGTTTCA CCACCATCTC TGCCCTCAGT GAACCCATCG AAGTGGTCGA TCTGCTTAAT 
GACCTCTACA CACACTTTGA TGGAATCATC GCTATCCACG ATGTCTATAA GGTGGAGACT 
ATTGGTGATG CGTACATGGT GGCATCAGGA GTTCCCAATC GCAATGGTAC CCGCCACGCT 
GCAGAGATGG CCAACATGTC CCTGGACATT CTCCACTGCA TCGGTACATT TAAAATGAGG 
CACATGCCTG ACGTCAAAGT CAAAATTCGC ATTGGACTAC ATTCTGGTCC AGTGGTCGCA 
GGTGTAGTTG GCTTAAAGAT GCCTCGATAC TGTCTGTTCG GGGACACAGT AAACACAGCC 
TCCCGAATGG AATCCACAGG GTTGCCTTAC AGAATTCATG TCAACCAGAG TACAGTTGAT 
GTCCTAAACA GTCTCAAACT TGGCTACAAA ATAGATACTC GGGGCAGGAC CGAGCTCAAG 
GGTAAAGGTG TAGAAGAAAC ATTTTGGTTG GTTGGGAGGG ATGGATTTGA CAAGCCGTTA 
CCTATACCGC CAGACCTTAC TCCTGGGGCG AGCAACCATG GTATCAGCTT GGATGAGATC 
CCGACTGATA GGCGACAGAA ATTCTTGGAT CGACAGAAGA GGATGGGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2d ( XM_005165262.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165319.1 Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa02391
Clone ID Related Accession (Same CDS sequence)	XM_005165262.2 , NM_131866.1
Accession Version	XM_005165262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	guanylyl cyclase 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005165262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTTTCTCCA TTTTTTGGGA CCATATATAA CATGATCTAT 
TATACAGCCA TGGCTGCAGA GCAAAGCCGA GCTTCGAGTG GTGGGCGATG GGTATCGGGC 
GATTTACTGT CTCAAAACGA GGGTGGATTT GAATTTGATG GCTTTAACCA GCGGATATCG 
GGAGGTACTA ACGGAGAGGG AATGCAGGTC CGCTATGCTG TCCTGGATTC AGACGGCTCT 
GGGTCCAGCC TGTATCGCAC ACACGCCCTG GAAGCTTCAC ACACATATGG AAAATATGGA 
GGGTTAAAAT ACTTGGGGAA GTCAATTCAT TTTGCTGGAG CGTCTCCGAG CAGTGACGCG 
AACTGCTGGT TTAGTCCTTA TATCGCATGT AGTGGAGGAT TGGATGGAGT GACGCTTTTC 
TTCTTGCTCA TGTTTTTCAT AGTGTTGATA GCGGGTGCAG GCATCCTTTT TCTACAAGTT 
AGAAAATATG GGGCTGGAGG TTTTTTTGGT GCGTTTATTG GAGGAGGAGG AAGCCCCACT 
AAGATAGTGC TGACGCTGGA TGACTTGGTC TTCATCAACA CCACACTCAG CAGAAGGAAA 
CTGAATGACG AGAGTATGCT GAAGAGTCAG CTAGATGTTA AAACCCCGCG CCACTCAGTC 
TCTGGTCGCA GTTACATCAC ATCAACCCCA GAAACATCCA ATGTTGCTGT ATTTGAGGGT 
GACTGGGTCT GGTTAAAGAA ATGCCCCTGT GCAGATTCTA TTACAGAGAT CAGTGAAAGC 
ACACAGACCA TCTTTGTCAA GCTGAGAGAT ATGCGTCATG AGAATCTGAA CTTGTTCCTG 
GGTCTGTTTA TGGATACTGG TATATTCGGC ATTGTTACAG AGCACTGCAC CAGAGGCAGT 
CTGGAAGACC TGCTCAACAA TGAGGAAATG AGGCTTGACT GGATGTTCAA ATCCTCTCTT 
CTCCTCGACC TGATTAGGGG AATGAAGTAC TTGCATAATC GGGGCATTAT TCATGGACGC 
CTGAAGTCTC GGAACTGTGT GGTGGATGGT CGATTTGTTT TGAAGGTTAC AGATTATGGC 
TTCAATGAAA TCCTCAACTG CCAAAACATC ATCCTGGAGG ACAACGCACC AGAGGATCAG 
TTCTGGACAG CTCCGGAGAT CCTGAGGAAT CCTGATCTGA AGAAGAAGGG AACATATCCA 
GGAGATGTAT ACAGCTTTTC TATCATCATG CAGGAGGTGA TCTCTCGCTG CGCACCCTTC 
TGCATGTTGG ATATGCCTCC TGAAGAAATC ATAGAAAAGG TGCGCTCTCC TCCACCACTG 
TGCCGACCCA CTGTGTCTAT GGATGAAGCT CCGCTAGATG TGATTCAGCT CATGAAGCAG 
GCCTGGAGTG AGGAGCCTGA ACAGAGGCCT ACGTTTGAGG ATATATTCAG ACAGTTTAAG 
AGCATGAACA AAGGAAAGAA GACTAACATA ATAGACTCAA TGTTGAGGAT GCTGGAGCAG 
TACTCCTCAA ACCTGGAGGA TCTGATCCGA GAGAGGACCG AAGAACTGGA GGTAGAGCGA 
CAGAAAACTG ATGCTCTTCT AGCCCAGATG CTGCCCAAGT CGGTGGCCTT GGCATTAAAA 
ACAGGGAAGC CAGTGAAGCC AGAGCATTTC GCTGAAGTCA CACTGTATTT CAGTGATATC 
GTGGGTTTCA CCACCATCTC TGCCCTCAGT GAACCCATCG AAGTGGTCGA TCTGCTTAAT 
GACCTCTACA CACACTTTGA TGGAATCATC GCTATCCACG ATGTCTATAA GGTGGAGACT 
ATTGGTGATG CGTACATGGT GGCATCAGGA GTTCCCAATC GCAATGGTAC CCGCCACGCT 
GCAGAGATGG CCAACATGTC CCTGGACATT CTCCACTGCA TCGGTACATT TAAAATGAGG 
CACATGCCTG ACGTCAAAGT CAAAATTCGC ATTGGACTAC ATTCTGGTCC AGTGGTCGCA 
GGTGTAGTTG GCTTAAAGAT GCCTCGATAC TGTCTGTTCG GGGACACAGT AAACACAGCC 
TCCCGAATGG AATCCACAGG GTTGCCTTAC AGAATTCATG TCAACCAGAG TACAGTTGAT 
GTCCTAAACA GTCTCAAACT TGGCTACAAA ATAGATACTC GGGGCAGGAC CGAGCTCAAG 
GGTAAAGGTG TAGAAGAAAC ATTTTGGTTG GTTGGGAGGG ATGGATTTGA CAAGCCGTTA 
CCTATACCGC CAGACCTTAC TCCTGGGGCG AGCAACCATG GTATCAGCTT GGATGAGATC 
CCGACTGATA GGCGACAGAA ATTCTTGGAT CGACAGAAGA GGATGGGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165319.1
CDS	645..4058
Translation	MNDHTSFLCQSHHPRWQTQSSAGKRRRKQSSSQKSETPASPVSSSPVLRSHSWWGNCFLLPLVMLSYFPCEAWGTTFKIAIVGPWACDPMYSKALPDLAARLAMSRINKDPELNKGYWYDYTVINEDCSSSRGLARFAELEGYGSAFLGPANPGYCTAATLLAKNWNKGILSWGCLKAEMDEEMYPTFLRPLPLSSRVLFSVLRFFRWAHVGIVTAEGDMWEATGHELAASLRALGLPVGKVVTMEQDKEGPRQALQAIRDTDRVRVVIMCMHSALIGGEEQYQLLTTALEMRMIDRGYIFIPYDTLLYSLPYENVKYPILANDSKLRRAYDGVLTVTMDQGEQNFYEAFNAAQESFEIRGSTSAEEVSPFFGTIYNMIYYTAMAAEQSRASSGGRWVSGDLLSQNEGGFEFDGFNQRISGGTNGEGMQVRYAVLDSDGSGSSLYRTHALEASHTYGKYGGLKYLGKSIHFAGASPSSDANCWFSPYIACSGGLDGVTLFFLLMFFIVLIAGAGILFLQVRKYGAGGFFGAFIGGGGSPTKIVLTLDDLVFINTTLSRRKLNDESMLKSQLDVKTPRHSVSGRSYITSTPETSNVAVFEGDWVWLKKCPCADSITEISESTQTIFVKLRDMRHENLNLFLGLFMDTGIFGIVTEHCTRGSLEDLLNNEEMRLDWMFKSSLLLDLIRGMKYLHNRGIIHGRLKSRNCVVDGRFVLKVTDYGFNEILNCQNIILEDNAPEDQFWTAPEILRNPDLKKKGTYPGDVYSFSIIMQEVISRCAPFCMLDMPPEEIIEKVRSPPPLCRPTVSMDEAPLDVIQLMKQAWSEEPEQRPTFEDIFRQFKSMNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEVERQKTDALLAQMLPKSVALALKTGKPVKPEHFAEVTLYFSDIVGFTTISALSEPIEVVDLLNDLYTHFDGIIAIHDVYKVETIGDAYMVASGVPNRNGTRHAAEMANMSLDILHCIGTFKMRHMPDVKVKIRIGLHSGPVVAGVVGLKMPRYCLFGDTVNTASRMESTGLPYRIHVNQSTVDVLNSLKLGYKIDTRGRTELKGKGVEETFWLVGRDGFDKPLPIPPDLTPGASNHGISLDEIPTDRRQKFLDRQKRMGN

Target ORF information:

RefSeq Version	XM_005165262.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165262.2

ORF Insert Sequence:

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTTTCTCCA TTTTTTGGGA CCATATATAA CATGATCTAT 
TATACAGCCA TGGCTGCAGA GCAAAGCCGA GCTTCGAGTG GTGGGCGATG GGTATCGGGC 
GATTTACTGT CTCAAAACGA GGGTGGATTT GAATTTGATG GCTTTAACCA GCGGATATCG 
GGAGGTACTA ACGGAGAGGG AATGCAGGTC CGCTATGCTG TCCTGGATTC AGACGGCTCT 
GGGTCCAGCC TGTATCGCAC ACACGCCCTG GAAGCTTCAC ACACATATGG AAAATATGGA 
GGGTTAAAAT ACTTGGGGAA GTCAATTCAT TTTGCTGGAG CGTCTCCGAG CAGTGACGCG 
AACTGCTGGT TTAGTCCTTA TATCGCATGT AGTGGAGGAT TGGATGGAGT GACGCTTTTC 
TTCTTGCTCA TGTTTTTCAT AGTGTTGATA GCGGGTGCAG GCATCCTTTT TCTACAAGTT 
AGAAAATATG GGGCTGGAGG TTTTTTTGGT GCGTTTATTG GAGGAGGAGG AAGCCCCACT 
AAGATAGTGC TGACGCTGGA TGACTTGGTC TTCATCAACA CCACACTCAG CAGAAGGAAA 
CTGAATGACG AGAGTATGCT GAAGAGTCAG CTAGATGTTA AAACCCCGCG CCACTCAGTC 
TCTGGTCGCA GTTACATCAC ATCAACCCCA GAAACATCCA ATGTTGCTGT ATTTGAGGGT 
GACTGGGTCT GGTTAAAGAA ATGCCCCTGT GCAGATTCTA TTACAGAGAT CAGTGAAAGC 
ACACAGACCA TCTTTGTCAA GCTGAGAGAT ATGCGTCATG AGAATCTGAA CTTGTTCCTG 
GGTCTGTTTA TGGATACTGG TATATTCGGC ATTGTTACAG AGCACTGCAC CAGAGGCAGT 
CTGGAAGACC TGCTCAACAA TGAGGAAATG AGGCTTGACT GGATGTTCAA ATCCTCTCTT 
CTCCTCGACC TGATTAGGGG AATGAAGTAC TTGCATAATC GGGGCATTAT TCATGGACGC 
CTGAAGTCTC GGAACTGTGT GGTGGATGGT CGATTTGTTT TGAAGGTTAC AGATTATGGC 
TTCAATGAAA TCCTCAACTG CCAAAACATC ATCCTGGAGG ACAACGCACC AGAGGATCAG 
TTCTGGACAG CTCCGGAGAT CCTGAGGAAT CCTGATCTGA AGAAGAAGGG AACATATCCA 
GGAGATGTAT ACAGCTTTTC TATCATCATG CAGGAGGTGA TCTCTCGCTG CGCACCCTTC 
TGCATGTTGG ATATGCCTCC TGAAGAAATC ATAGAAAAGG TGCGCTCTCC TCCACCACTG 
TGCCGACCCA CTGTGTCTAT GGATGAAGCT CCGCTAGATG TGATTCAGCT CATGAAGCAG 
GCCTGGAGTG AGGAGCCTGA ACAGAGGCCT ACGTTTGAGG ATATATTCAG ACAGTTTAAG 
AGCATGAACA AAGGAAAGAA GACTAACATA ATAGACTCAA TGTTGAGGAT GCTGGAGCAG 
TACTCCTCAA ACCTGGAGGA TCTGATCCGA GAGAGGACCG AAGAACTGGA GGTAGAGCGA 
CAGAAAACTG ATGCTCTTCT AGCCCAGATG CTGCCCAAGT CGGTGGCCTT GGCATTAAAA 
ACAGGGAAGC CAGTGAAGCC AGAGCATTTC GCTGAAGTCA CACTGTATTT CAGTGATATC 
GTGGGTTTCA CCACCATCTC TGCCCTCAGT GAACCCATCG AAGTGGTCGA TCTGCTTAAT 
GACCTCTACA CACACTTTGA TGGAATCATC GCTATCCACG ATGTCTATAA GGTGGAGACT 
ATTGGTGATG CGTACATGGT GGCATCAGGA GTTCCCAATC GCAATGGTAC CCGCCACGCT 
GCAGAGATGG CCAACATGTC CCTGGACATT CTCCACTGCA TCGGTACATT TAAAATGAGG 
CACATGCCTG ACGTCAAAGT CAAAATTCGC ATTGGACTAC ATTCTGGTCC AGTGGTCGCA 
GGTGTAGTTG GCTTAAAGAT GCCTCGATAC TGTCTGTTCG GGGACACAGT AAACACAGCC 
TCCCGAATGG AATCCACAGG GTTGCCTTAC AGAATTCATG TCAACCAGAG TACAGTTGAT 
GTCCTAAACA GTCTCAAACT TGGCTACAAA ATAGATACTC GGGGCAGGAC CGAGCTCAAG 
GGTAAAGGTG TAGAAGAAAC ATTTTGGTTG GTTGGGAGGG ATGGATTTGA CAAGCCGTTA 
CCTATACCGC CAGACCTTAC TCCTGGGGCG AGCAACCATG GTATCAGCTT GGATGAGATC 
CCGACTGATA GGCGACAGAA ATTCTTGGAT CGACAGAAGA GGATGGGCAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2d ( XM_021475989.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331664.1 Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.

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CloneID	ODa10659
Clone ID Related Accession (Same CDS sequence)	XM_021475989.1 , XM_005165261.3 , XM_005165261.2
Accession Version	XM_021475989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	guanylyl cyclase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331664.1
CDS	528..3953
Translation	MNDHTSFLCQSHHPRWQTQSSAGKRRRKQSSSQKSETPASPVSSSPVLRSHSWWGNCFLLPLVMLSYFPCEAWGTTFKIAIVGPWACDPMYSKALPDLAARLAMSRINKDPELNKGYWYDYTVINEDCSSSRGLARFAELEGYGSAFLGPANPGYCTAATLLAKNWNKGILSWGCLKAEMDEEMYPTFLRPLPLSSRVLFSVLRFFRWAHVGIVTAEGDMWEATGHELAASLRALGLPVGKVVTMEQDKEGPRQALQAIRDTDRVRVVIMCMHSALIGGEEQYQLLTTALEMRMIDRGYIFIPYDTLLYSLPYENVKYPILANDSKLRRAYDGVLTVTMDQGEQNFYEAFNAAQESFEIRGSTSAEEVTRWVSPFFGTIYNMIYYTAMAAEQSRASSGGRWVSGDLLSQNEGGFEFDGFNQRISGGTNGEGMQVRYAVLDSDGSGSSLYRTHALEASHTYGKYGGLKYLGKSIHFAGASPSSDANCWFSPYIACSGGLDGVTLFFLLMFFIVLIAGAGILFLQVRKYGAGGFFGAFIGGGGSPTKIVLTLDDLVFINTTLSRRKLNDESMLKSQLDVKTPRHSVSGRSYITSTPETSNVAVFEGDWVWLKKCPCADSITEISESTQTIFVKLRDMRHENLNLFLGLFMDTGIFGIVTEHCTRGSLEDLLNNEEMRLDWMFKSSLLLDLIRGMKYLHNRGIIHGRLKSRNCVVDGRFVLKVTDYGFNEILNCQNIILEDNAPEDQFWTAPEILRNPDLKKKGTYPGDVYSFSIIMQEVISRCAPFCMLDMPPEEIIEKVRSPPPLCRPTVSMDEAPLDVIQLMKQAWSEEPEQRPTFEDIFRQFKSMNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEVERQKTDALLAQMLPKSVALALKTGKPVKPEHFAEVTLYFSDIVGFTTISALSEPIEVVDLLNDLYTHFDGIIAIHDVYKVETIGDAYMVASGVPNRNGTRHAAEMANMSLDILHCIGTFKMRHMPDVKVKIRIGLHSGPVVAGVVGLKMPRYCLFGDTVNTASRMESTGLPYRIHVNQSTVDVLNSLKLGYKIDTRGRTELKGKGVEETFWLVGRDGFDKPLPIPPDLTPGASNHGISLDEIPTDRRQKFLDRQKRMGN

Target ORF information:

RefSeq Version	XM_021475989.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475989.1

ORF Insert Sequence:

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2d ( XM_005165261.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165318.1 Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10659
Clone ID Related Accession (Same CDS sequence)	XM_021475989.1 , XM_005165261.3 , XM_005165261.2
Accession Version	XM_005165261.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	guanylyl cyclase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165261.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165318.1
CDS	633..4058
Translation	MNDHTSFLCQSHHPRWQTQSSAGKRRRKQSSSQKSETPASPVSSSPVLRSHSWWGNCFLLPLVMLSYFPCEAWGTTFKIAIVGPWACDPMYSKALPDLAARLAMSRINKDPELNKGYWYDYTVINEDCSSSRGLARFAELEGYGSAFLGPANPGYCTAATLLAKNWNKGILSWGCLKAEMDEEMYPTFLRPLPLSSRVLFSVLRFFRWAHVGIVTAEGDMWEATGHELAASLRALGLPVGKVVTMEQDKEGPRQALQAIRDTDRVRVVIMCMHSALIGGEEQYQLLTTALEMRMIDRGYIFIPYDTLLYSLPYENVKYPILANDSKLRRAYDGVLTVTMDQGEQNFYEAFNAAQESFEIRGSTSAEEVTRWVSPFFGTIYNMIYYTAMAAEQSRASSGGRWVSGDLLSQNEGGFEFDGFNQRISGGTNGEGMQVRYAVLDSDGSGSSLYRTHALEASHTYGKYGGLKYLGKSIHFAGASPSSDANCWFSPYIACSGGLDGVTLFFLLMFFIVLIAGAGILFLQVRKYGAGGFFGAFIGGGGSPTKIVLTLDDLVFINTTLSRRKLNDESMLKSQLDVKTPRHSVSGRSYITSTPETSNVAVFEGDWVWLKKCPCADSITEISESTQTIFVKLRDMRHENLNLFLGLFMDTGIFGIVTEHCTRGSLEDLLNNEEMRLDWMFKSSLLLDLIRGMKYLHNRGIIHGRLKSRNCVVDGRFVLKVTDYGFNEILNCQNIILEDNAPEDQFWTAPEILRNPDLKKKGTYPGDVYSFSIIMQEVISRCAPFCMLDMPPEEIIEKVRSPPPLCRPTVSMDEAPLDVIQLMKQAWSEEPEQRPTFEDIFRQFKSMNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEVERQKTDALLAQMLPKSVALALKTGKPVKPEHFAEVTLYFSDIVGFTTISALSEPIEVVDLLNDLYTHFDGIIAIHDVYKVETIGDAYMVASGVPNRNGTRHAAEMANMSLDILHCIGTFKMRHMPDVKVKIRIGLHSGPVVAGVVGLKMPRYCLFGDTVNTASRMESTGLPYRIHVNQSTVDVLNSLKLGYKIDTRGRTELKGKGVEETFWLVGRDGFDKPLPIPPDLTPGASNHGISLDEIPTDRRQKFLDRQKRMGN

Target ORF information:

RefSeq Version	XM_005165261.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165261.3

ORF Insert Sequence:

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gucy2d ( XM_005165261.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165318.1 Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.

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CloneID	ODa10659
Clone ID Related Accession (Same CDS sequence)	XM_021475989.1 , XM_005165261.3 , XM_005165261.2
Accession Version	XM_005165261.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	guanylyl cyclase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005165261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165318.1
CDS	646..4071
Translation	MNDHTSFLCQSHHPRWQTQSSAGKRRRKQSSSQKSETPASPVSSSPVLRSHSWWGNCFLLPLVMLSYFPCEAWGTTFKIAIVGPWACDPMYSKALPDLAARLAMSRINKDPELNKGYWYDYTVINEDCSSSRGLARFAELEGYGSAFLGPANPGYCTAATLLAKNWNKGILSWGCLKAEMDEEMYPTFLRPLPLSSRVLFSVLRFFRWAHVGIVTAEGDMWEATGHELAASLRALGLPVGKVVTMEQDKEGPRQALQAIRDTDRVRVVIMCMHSALIGGEEQYQLLTTALEMRMIDRGYIFIPYDTLLYSLPYENVKYPILANDSKLRRAYDGVLTVTMDQGEQNFYEAFNAAQESFEIRGSTSAEEVTRWVSPFFGTIYNMIYYTAMAAEQSRASSGGRWVSGDLLSQNEGGFEFDGFNQRISGGTNGEGMQVRYAVLDSDGSGSSLYRTHALEASHTYGKYGGLKYLGKSIHFAGASPSSDANCWFSPYIACSGGLDGVTLFFLLMFFIVLIAGAGILFLQVRKYGAGGFFGAFIGGGGSPTKIVLTLDDLVFINTTLSRRKLNDESMLKSQLDVKTPRHSVSGRSYITSTPETSNVAVFEGDWVWLKKCPCADSITEISESTQTIFVKLRDMRHENLNLFLGLFMDTGIFGIVTEHCTRGSLEDLLNNEEMRLDWMFKSSLLLDLIRGMKYLHNRGIIHGRLKSRNCVVDGRFVLKVTDYGFNEILNCQNIILEDNAPEDQFWTAPEILRNPDLKKKGTYPGDVYSFSIIMQEVISRCAPFCMLDMPPEEIIEKVRSPPPLCRPTVSMDEAPLDVIQLMKQAWSEEPEQRPTFEDIFRQFKSMNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEVERQKTDALLAQMLPKSVALALKTGKPVKPEHFAEVTLYFSDIVGFTTISALSEPIEVVDLLNDLYTHFDGIIAIHDVYKVETIGDAYMVASGVPNRNGTRHAAEMANMSLDILHCIGTFKMRHMPDVKVKIRIGLHSGPVVAGVVGLKMPRYCLFGDTVNTASRMESTGLPYRIHVNQSTVDVLNSLKLGYKIDTRGRTELKGKGVEETFWLVGRDGFDKPLPIPPDLTPGASNHGISLDEIPTDRRQKFLDRQKRMGN

Target ORF information:

RefSeq Version	XM_005165261.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanylyl cyclase 3 (gc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165261.2

ORF Insert Sequence:

ATGAATGACC ACACAAGCTT TCTCTGCCAG TCCCACCACC CTCGATGGCA AACACAGTCT 
TCTGCTGGAA AGAGACGACG GAAACAATCC TCATCGCAAA AATCTGAAAC CCCAGCGAGC 
CCAGTGTCCT CGTCCCCAGT GCTACGCAGC CACAGCTGGT GGGGAAACTG CTTTCTCCTT 
CCTCTAGTCA TGCTTTCATA CTTTCCCTGT GAAGCCTGGG GCACCACCTT CAAAATTGCC 
ATTGTAGGAC CGTGGGCATG TGACCCCATG TACTCTAAAG CCCTGCCAGA TCTGGCTGCC 
CGATTAGCCA TGAGCCGCAT CAACAAAGAC CCCGAACTCA ATAAAGGCTA CTGGTATGAT 
TACACAGTCA TTAACGAGGA TTGCTCCAGC TCCAGAGGCC TGGCACGCTT TGCCGAGCTG 
GAGGGTTACG GTTCGGCTTT TCTAGGACCG GCAAACCCTG GCTACTGCAC GGCTGCCACA 
CTGCTGGCAA AGAACTGGAA CAAAGGCATC CTATCCTGGG GTTGCCTGAA GGCTGAGATG 
GATGAAGAGA TGTATCCCAC CTTTCTGCGG CCGCTGCCGT TGTCCTCCCG GGTGCTTTTC 
TCTGTGCTGC GGTTCTTCCG TTGGGCACAT GTTGGGATTG TAACTGCTGA GGGCGACATG 
TGGGAGGCAA CTGGGCATGA GCTTGCAGCA TCCCTGCGTG CTCTTGGCTT GCCCGTTGGT 
AAGGTTGTTA CGATGGAACA GGACAAAGAG GGGCCACGGC AAGCCCTACA GGCAATACGG 
GACACAGATC GAGTAAGGGT TGTCATCATG TGCATGCACT CTGCCCTGAT TGGTGGAGAG 
GAACAGTATC AGTTGCTGAC TACAGCATTG GAAATGAGAA TGATTGACCG AGGGTACATC 
TTCATTCCAT ACGACACCCT GCTCTATTCA CTGCCATATG AAAATGTAAA ATATCCAATC 
CTGGCCAATG ACAGCAAACT GCGACGAGCA TACGATGGGG TCTTAACAGT AACCATGGAC 
CAAGGGGAAC AAAACTTTTA TGAGGCCTTT AATGCAGCAC AGGAATCCTT TGAAATCCGC 
GGCAGCACAT CAGCTGAAGA GGTAACACGA TGGGTTTCTC CATTTTTTGG GACCATATAT 
AACATGATCT ATTATACAGC CATGGCTGCA GAGCAAAGCC GAGCTTCGAG TGGTGGGCGA 
TGGGTATCGG GCGATTTACT GTCTCAAAAC GAGGGTGGAT TTGAATTTGA TGGCTTTAAC 
CAGCGGATAT CGGGAGGTAC TAACGGAGAG GGAATGCAGG TCCGCTATGC TGTCCTGGAT 
TCAGACGGCT CTGGGTCCAG CCTGTATCGC ACACACGCCC TGGAAGCTTC ACACACATAT 
GGAAAATATG GAGGGTTAAA ATACTTGGGG AAGTCAATTC ATTTTGCTGG AGCGTCTCCG 
AGCAGTGACG CGAACTGCTG GTTTAGTCCT TATATCGCAT GTAGTGGAGG ATTGGATGGA 
GTGACGCTTT TCTTCTTGCT CATGTTTTTC ATAGTGTTGA TAGCGGGTGC AGGCATCCTT 
TTTCTACAAG TTAGAAAATA TGGGGCTGGA GGTTTTTTTG GTGCGTTTAT TGGAGGAGGA 
GGAAGCCCCA CTAAGATAGT GCTGACGCTG GATGACTTGG TCTTCATCAA CACCACACTC 
AGCAGAAGGA AACTGAATGA CGAGAGTATG CTGAAGAGTC AGCTAGATGT TAAAACCCCG 
CGCCACTCAG TCTCTGGTCG CAGTTACATC ACATCAACCC CAGAAACATC CAATGTTGCT 
GTATTTGAGG GTGACTGGGT CTGGTTAAAG AAATGCCCCT GTGCAGATTC TATTACAGAG 
ATCAGTGAAA GCACACAGAC CATCTTTGTC AAGCTGAGAG ATATGCGTCA TGAGAATCTG 
AACTTGTTCC TGGGTCTGTT TATGGATACT GGTATATTCG GCATTGTTAC AGAGCACTGC 
ACCAGAGGCA GTCTGGAAGA CCTGCTCAAC AATGAGGAAA TGAGGCTTGA CTGGATGTTC 
AAATCCTCTC TTCTCCTCGA CCTGATTAGG GGAATGAAGT ACTTGCATAA TCGGGGCATT 
ATTCATGGAC GCCTGAAGTC TCGGAACTGT GTGGTGGATG GTCGATTTGT TTTGAAGGTT 
ACAGATTATG GCTTCAATGA AATCCTCAAC TGCCAAAACA TCATCCTGGA GGACAACGCA 
CCAGAGGATC AGTTCTGGAC AGCTCCGGAG ATCCTGAGGA ATCCTGATCT GAAGAAGAAG 
GGAACATATC CAGGAGATGT ATACAGCTTT TCTATCATCA TGCAGGAGGT GATCTCTCGC 
TGCGCACCCT TCTGCATGTT GGATATGCCT CCTGAAGAAA TCATAGAAAA GGTGCGCTCT 
CCTCCACCAC TGTGCCGACC CACTGTGTCT ATGGATGAAG CTCCGCTAGA TGTGATTCAG 
CTCATGAAGC AGGCCTGGAG TGAGGAGCCT GAACAGAGGC CTACGTTTGA GGATATATTC 
AGACAGTTTA AGAGCATGAA CAAAGGAAAG AAGACTAACA TAATAGACTC AATGTTGAGG 
ATGCTGGAGC AGTACTCCTC AAACCTGGAG GATCTGATCC GAGAGAGGAC CGAAGAACTG 
GAGGTAGAGC GACAGAAAAC TGATGCTCTT CTAGCCCAGA TGCTGCCCAA GTCGGTGGCC 
TTGGCATTAA AAACAGGGAA GCCAGTGAAG CCAGAGCATT TCGCTGAAGT CACACTGTAT 
TTCAGTGATA TCGTGGGTTT CACCACCATC TCTGCCCTCA GTGAACCCAT CGAAGTGGTC 
GATCTGCTTA ATGACCTCTA CACACACTTT GATGGAATCA TCGCTATCCA CGATGTCTAT 
AAGGTGGAGA CTATTGGTGA TGCGTACATG GTGGCATCAG GAGTTCCCAA TCGCAATGGT 
ACCCGCCACG CTGCAGAGAT GGCCAACATG TCCCTGGACA TTCTCCACTG CATCGGTACA 
TTTAAAATGA GGCACATGCC TGACGTCAAA GTCAAAATTC GCATTGGACT ACATTCTGGT 
CCAGTGGTCG CAGGTGTAGT TGGCTTAAAG ATGCCTCGAT ACTGTCTGTT CGGGGACACA 
GTAAACACAG CCTCCCGAAT GGAATCCACA GGGTTGCCTT ACAGAATTCA TGTCAACCAG 
AGTACAGTTG ATGTCCTAAA CAGTCTCAAA CTTGGCTACA AAATAGATAC TCGGGGCAGG 
ACCGAGCTCA AGGGTAAAGG TGTAGAAGAA ACATTTTGGT TGGTTGGGAG GGATGGATTT 
GACAAGCCGT TACCTATACC GCCAGACCTT ACTCCTGGGG CGAGCAACCA TGGTATCAGC 
TTGGATGAGA TCCCGACTGA TAGGCGACAG AAATTCTTGG ATCGACAGAA GAGGATGGGC 
AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcripts within rod photoreceptors of the Zebrafish retina.
BMC genomics19(1)127(2018 02)
Sun C,Galicia C,Stenkamp DL

Aipl1 is required for cone photoreceptor function and survival through the stability of Pde6c and Gc3 in zebrafish.
Scientific reports745962(2017 04)
Iribarne M,Nishiwaki Y,Nakamura S,Araragi M,Oguri E,Masai I

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array.
BMC genomics16969(2015 Nov)
Tsai HY,Hamilton A,Tinch AE,Guy DR,Gharbi K,Stear MJ,Matika O,Bishop SC,Houston RD

Molecular and neuronal homology between the olfactory systems of zebrafish and mouse.
Scientific reports511487(2015 Jun)
Saraiva LR,Ahuja G,Ivandic I,Syed AS,Marioni JC,Korsching SI,Logan DW

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gucy2d cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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