arhgef5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef5
Entrez Gene ID	100537160
Full Name	Rho guanine nucleotide exchange factor (GEF) 5
General protein information	Preferred Names Rho guanine nucleotide exchange factor (GEF) 5 Names uncharacterized protein arhgef5 zinc finger CCCH domain-containing protein 13 zmp:0000000797
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161483.3	XP_005161540.1	uncharacterized protein arhgef5 isoform X2
XM_003200169.5	XP_003200217.3	zinc finger CCCH domain-containing protein 13 isoform X1
XM_003200169.4	XP_003200217.2	zinc finger CCCH domain-containing protein 13-like isoform X1
XM_005161483.4	XP_005161540.2	uncharacterized protein arhgef5 isoform X2

Pathways from BioSystems
Gene Ontology

The following arhgef5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149371	XM_003200169.5 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa149372	XM_005161483.4 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa21305	XM_003200169.4 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa21306	XM_005161483.3 Latest version!	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef5 ( XM_003200169.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200217.3 Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149371
Clone ID Related Accession (Same CDS sequence)	XM_003200169.5
Accession Version	XM_003200169.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	zinc finger CCCH domain-containing protein 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200169.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGCATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCTAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCAAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGGTAGTGTG 
ACACACATAG ACATACAAAT AAACACACAA ACAGAAACTG ACAGAGAAAT TAACATATCG 
AGGGAACAGG AAGAACTAAT ACAGGGAGAA GAGAAACAAC AAGGAGAGAT TGAAAAGGAG 
TCAGATGAGG AAACTATCTT GTTGGATCCA AGTTGCATTG AACAGACAGT TTCCTCAAAA 
AGAGAGGCAG ATAGGACAGT AGAAGGACAC GTTTCTAAGA GGAAAGATAG CATAGAGAAT 
GTCATTCTTG GTTCAAGTAG CTTTCGAGAA TTGGGGAATG AGGCCCGCAT AAGGAGGAGG 
GGTATCCGAA AAACAACAGA GGGAACCAAA GAAGAGGAAG AGGATGAGGA AGAGGGAGGA 
GTTGGAAGGG ACCGCAGGAC CAGAGTATTC AACAATTCAG ATGCTGAGGA GGATGAATTA 
TGTTTGACCT GGAGTGAAGG GGATCTAAGG AAACTTGGGA GGACAAAAAA GAGGAGTTCT 
AAATACTTTA ACTCTCAACT GTATCAGGAG TACAGTGAGG TCGTCCAGAA CAGAGAAATT 
CTGCTGACTA ATTATGATAC TACATCGCTA AGTGGAGATA ATATCACCAT CCGTTCATCT 
TCCCCTCTGA ATCATTCGCC AAAGCTATCT CACAGACCTC TCCCAGCGCT TCCTCAAGTC 
CACCTGCATC CCCACTCACT GTCCTTGAAT AATTCCCTAA AAAGTCTTAA TTTACCTCAG 
CAATCTATGA ACAGACCCCC TTCTCCTCGC CTGTCTATCT CCGCTTCCTC ACCCACACTG 
TGGCAGGATC TCCCTGGAGT CAGGAACAGC CCAGAAATTG TGGAACTTAC TGAAGATGAG 
AGACGCCTGC AGGAGGTGAG GTTTGAGGTT GTGACATCTG AGGCATCATA CTGTCGTAGT 
TTGGACATCG TAGTGGAGAA CTTTGTCCTG TCTAAACAGC TCAATGCTAT CCTGTCATCA 
CAAGACAAAA ATTGGCTGTT CTCTAGGCTA AATGATGTTA GAGCCATCAG TCACAGTTTT 
ATGTCTCACC TTGAAGAAAA CGTGGAAAGA GATATGTTGC ACTTTACAGT CTGTGACATC 
ATTATTAAAT ACTGTCCCCG ATTCCGAAAT GTGTATGTGC CATATCTTAC CAACCAATCA 
TATCAGGACA AGACGTATCA AAGATTAATG GATGAGAGTC AGGAATTTCG CAGAGTTGTG 
GAAAAGTTAG AGCGTAATCC AGTGTGCCAG CGGTTGCCTT TACGGTCATT TCTCATTCTT 
CCGTTCCAAC GAATTACACG CCTCAAACTA TTAGTGCAGA ATATTGTAAA GAGAACTGCT 
CCAAAAACCA AAGATGAAGT GCAGGCCATT AAGGCCATGA AACTACTTGA GAAGATGATC 
CTGGATAGTA ATGAAAGTAT TTCTCAAATG AAAAACATTG AGTCACTTGT GACTCTTAAT 
GCCAAAGTTG ACTTTGAGTG CAGGACTCTT CCATTGATAA GTCAGTCCCG CAGATTAGTA 
CGAGAAGGGC CTGTGACTGA ACTGAGAGAC TTTTCCTTGA AAGACAGAGA AGAAGAGAGA 
AGTGTTTATA TGCACCTTTT TAATGACTAC CTCTTGCTCT CTATACGGAA AGAAGGGGGT 
AGGTTTACAG TTATCGACCA TGCTCCTGTA TCAGAGCTGC GGGCCGAGAA CTGCAGGTTC 
AAACTACATT CCTTACAGAA AAACCTGTTC CGTCTGCACA TGCCACAAAA AGCCTTGCTT 
TTGCGAACAG ATACCCAAGC TGATAATCTG CGCTGGATAT CAGCGCTCTC ACGTCCTTAT 
CCTGAGATTG ACTTCGGTGC AGTTCAGGAA ACTCCACAAA TGCAGTGCAT CAGAGCATAT 
GTTGCCCAGC AACCAGATGA ACTCAGTTTA GAGAAAGCTG ATGTCCTTGT AGTTCACCAG 
CAAAGTAGTG ATGGCTGGGT GGAAGGTACT CGTTTGTCAG ACAGGCAGCG TGGCTGGGCT 
CCTGAGCTTC ACCTGGAGAC AATAACCAGT GACAAAGCAA GGAAGCGCAA CCTGCTGGAT 
ACCATGAAAA TTGCTACTGC TGCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200217.3
CDS	779..5248
Translation	MDRERAMMQDSCDGTRERDRLPAIRRREMDWRERERWRDGDQYANGKSSLFEHTVHGERDNESKKGDTFPRMTKANRERDRRIPLSENERQRRLGRPKDTTYEDEKEREKRRYRDMDTRRHMKEHSDKLREQEFELGIRGRRREGHTGTNDPTGMREKETDRERDRYRQRDREVRYYPRPIERETESFSDKEWRTERQNGAERSRKRDTKSEGDSDGDRGKEMNREAVTKPGHKLYKSEGDSKADQRVDRVREKDRRRDQEAWRYKEMVRPREKDYRETGYYDKNGMRKDKRPDRDAYSNTPERGRYRNAELEKDGYSEREIEDRERWRRQNREPYRDKMEKSGQRTGGKISGVEIEKEHNRSVGENLEDRNRKNTSESEKEKLKGRTDGEQEVRIAPREEEGRQIVVEETVTKKFTGRIMNRWLEPRREKNPSLLEEDYRDRERARERYNQRFKESTKYEEDPARNNESKRTSAKDKYRAGEFMGDIDVESNEKLDKQEDMVNDKEAVEPEWRAKREIGGNNMEVNEREEERSIDRHDYSGIEEGSEGESRAEKDRIISADDGFVTVSSGGDIEDCMDFRNGEVPTDISGCSEATQGEEREPLRVFCVIGLTPIESQTKQNPYIDTASEEATDQHSDQNIDIQGVMFIEDANEAFEKFEHKESDIEGAMDDLALSANEEKVEQTGDNDQGTDDITSLKGQMIPSKEGAEEEPAEPSVTLDPCNDDGPETIVAPSEDALHRREMEATGNTEEHFDSDDTWSTCEERKRCSTAPHLTWAKNVVKEILGPNSEDTEHMRGSVTHIDIQINTQTETDREINISREQEELIQGEEKQQGEIEKESDEETILLDPSCIEQTVSSKREADRTVEGHVSKRKDSIENVILGSSSFRELGNEARIRRRGIRKTTEGTKEEEEDEEEGGVGRDRRTRVFNNSDAEEDELCLTWSEGDLRKLGRTKKRSSKYFNSQLYQEYSEVVQNREILLTNYDTTSLSGDNITIRSSSPLNHSPKLSHRPLPALPQVHLHPHSLSLNNSLKSLNLPQQSMNRPPSPRLSISASSPTLWQDLPGVRNSPEIVELTEDERRLQEVRFEVVTSEASYCRSLDIVVENFVLSKQLNAILSSQDKNWLFSRLNDVRAISHSFMSHLEENVERDMLHFTVCDIIIKYCPRFRNVYVPYLTNQSYQDKTYQRLMDESQEFRRVVEKLERNPVCQRLPLRSFLILPFQRITRLKLLVQNIVKRTAPKTKDEVQAIKAMKLLEKMILDSNESISQMKNIESLVTLNAKVDFECRTLPLISQSRRLVREGPVTELRDFSLKDREEERSVYMHLFNDYLLLSIRKEGGRFTVIDHAPVSELRAENCRFKLHSLQKNLFRLHMPQKALLLRTDTQADNLRWISALSRPYPEIDFGAVQETPQMQCIRAYVAQQPDELSLEKADVLVVHQQSSDGWVEGTRLSDRQRGWAPELHLETITSDKARKRNLLDTMKIATAAM

Target ORF information:

RefSeq Version	XM_003200169.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200169.5

ORF Insert Sequence:

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGCATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCTAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCAAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGGTAGTGTG 
ACACACATAG ACATACAAAT AAACACACAA ACAGAAACTG ACAGAGAAAT TAACATATCG 
AGGGAACAGG AAGAACTAAT ACAGGGAGAA GAGAAACAAC AAGGAGAGAT TGAAAAGGAG 
TCAGATGAGG AAACTATCTT GTTGGATCCA AGTTGCATTG AACAGACAGT TTCCTCAAAA 
AGAGAGGCAG ATAGGACAGT AGAAGGACAC GTTTCTAAGA GGAAAGATAG CATAGAGAAT 
GTCATTCTTG GTTCAAGTAG CTTTCGAGAA TTGGGGAATG AGGCCCGCAT AAGGAGGAGG 
GGTATCCGAA AAACAACAGA GGGAACCAAA GAAGAGGAAG AGGATGAGGA AGAGGGAGGA 
GTTGGAAGGG ACCGCAGGAC CAGAGTATTC AACAATTCAG ATGCTGAGGA GGATGAATTA 
TGTTTGACCT GGAGTGAAGG GGATCTAAGG AAACTTGGGA GGACAAAAAA GAGGAGTTCT 
AAATACTTTA ACTCTCAACT GTATCAGGAG TACAGTGAGG TCGTCCAGAA CAGAGAAATT 
CTGCTGACTA ATTATGATAC TACATCGCTA AGTGGAGATA ATATCACCAT CCGTTCATCT 
TCCCCTCTGA ATCATTCGCC AAAGCTATCT CACAGACCTC TCCCAGCGCT TCCTCAAGTC 
CACCTGCATC CCCACTCACT GTCCTTGAAT AATTCCCTAA AAAGTCTTAA TTTACCTCAG 
CAATCTATGA ACAGACCCCC TTCTCCTCGC CTGTCTATCT CCGCTTCCTC ACCCACACTG 
TGGCAGGATC TCCCTGGAGT CAGGAACAGC CCAGAAATTG TGGAACTTAC TGAAGATGAG 
AGACGCCTGC AGGAGGTGAG GTTTGAGGTT GTGACATCTG AGGCATCATA CTGTCGTAGT 
TTGGACATCG TAGTGGAGAA CTTTGTCCTG TCTAAACAGC TCAATGCTAT CCTGTCATCA 
CAAGACAAAA ATTGGCTGTT CTCTAGGCTA AATGATGTTA GAGCCATCAG TCACAGTTTT 
ATGTCTCACC TTGAAGAAAA CGTGGAAAGA GATATGTTGC ACTTTACAGT CTGTGACATC 
ATTATTAAAT ACTGTCCCCG ATTCCGAAAT GTGTATGTGC CATATCTTAC CAACCAATCA 
TATCAGGACA AGACGTATCA AAGATTAATG GATGAGAGTC AGGAATTTCG CAGAGTTGTG 
GAAAAGTTAG AGCGTAATCC AGTGTGCCAG CGGTTGCCTT TACGGTCATT TCTCATTCTT 
CCGTTCCAAC GAATTACACG CCTCAAACTA TTAGTGCAGA ATATTGTAAA GAGAACTGCT 
CCAAAAACCA AAGATGAAGT GCAGGCCATT AAGGCCATGA AACTACTTGA GAAGATGATC 
CTGGATAGTA ATGAAAGTAT TTCTCAAATG AAAAACATTG AGTCACTTGT GACTCTTAAT 
GCCAAAGTTG ACTTTGAGTG CAGGACTCTT CCATTGATAA GTCAGTCCCG CAGATTAGTA 
CGAGAAGGGC CTGTGACTGA ACTGAGAGAC TTTTCCTTGA AAGACAGAGA AGAAGAGAGA 
AGTGTTTATA TGCACCTTTT TAATGACTAC CTCTTGCTCT CTATACGGAA AGAAGGGGGT 
AGGTTTACAG TTATCGACCA TGCTCCTGTA TCAGAGCTGC GGGCCGAGAA CTGCAGGTTC 
AAACTACATT CCTTACAGAA AAACCTGTTC CGTCTGCACA TGCCACAAAA AGCCTTGCTT 
TTGCGAACAG ATACCCAAGC TGATAATCTG CGCTGGATAT CAGCGCTCTC ACGTCCTTAT 
CCTGAGATTG ACTTCGGTGC AGTTCAGGAA ACTCCACAAA TGCAGTGCAT CAGAGCATAT 
GTTGCCCAGC AACCAGATGA ACTCAGTTTA GAGAAAGCTG ATGTCCTTGT AGTTCACCAG 
CAAAGTAGTG ATGGCTGGGT GGAAGGTACT CGTTTGTCAG ACAGGCAGCG TGGCTGGGCT 
CCTGAGCTTC ACCTGGAGAC AATAACCAGT GACAAAGCAA GGAAGCGCAA CCTGCTGGAT 
ACCATGAAAA TTGCTACTGC TGCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef5 ( XM_005161483.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161540.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa149372
Clone ID Related Accession (Same CDS sequence)	XM_005161483.4
Accession Version	XM_005161483.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein arhgef5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161483.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGCATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCTAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCAAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGATGCTGAG 
GAGGATGAAT TATGTTTGAC CTGGAGTGAA GGGGATCTAA GGAAACTTGG GAGGACAAAA 
AAGAGGAGTT CTAAATACTT TAACTCTCAA CTGTATCAGG AGTACAGTGA GGTCGTCCAG 
AACAGAGAAA TTCTGCTGAC TAATTATGAT ACTACATCGC TAAGTGGAGA TAATATCACC 
ATCCGTTCAT CTTCCCCTCT GAATCATTCG CCAAAGCTAT CTCACAGACC TCTCCCAGCG 
CTTCCTCAAG TCCACCTGCA TCCCCACTCA CTGTCCTTGA ATAATTCCCT AAAAAGTCTT 
AATTTACCTC AGCAATCTAT GAACAGACCC CCTTCTCCTC GCCTGTCTAT CTCCGCTTCC 
TCACCCACAC TGTGGCAGGA TCTCCCTGGA GTCAGGAACA GCCCAGAAAT TGTGGAACTT 
ACTGAAGATG AGAGACGCCT GCAGGAGGTG AGGTTTGAGG TTGTGACATC TGAGGCATCA 
TACTGTCGTA GTTTGGACAT CGTAGTGGAG AACTTTGTCC TGTCTAAACA GCTCAATGCT 
ATCCTGTCAT CACAAGACAA AAATTGGCTG TTCTCTAGGC TAAATGATGT TAGAGCCATC 
AGTCACAGTT TTATGTCTCA CCTTGAAGAA AACGTGGAAA GAGATATGTT GCACTTTACA 
GTCTGTGACA TCATTATTAA ATACTGTCCC CGATTCCGAA ATGTGTATGT GCCATATCTT 
ACCAACCAAT CATATCAGGA CAAGACGTAT CAAAGATTAA TGGATGAGAG TCAGGAATTT 
CGCAGAGTTG TGGAAAAGTT AGAGCGTAAT CCAGTGTGCC AGCGGTTGCC TTTACGGTCA 
TTTCTCATTC TTCCGTTCCA ACGAATTACA CGCCTCAAAC TATTAGTGCA GAATATTGTA 
AAGAGAACTG CTCCAAAAAC CAAAGATGAA GTGCAGGCCA TTAAGGCCAT GAAACTACTT 
GAGAAGATGA TCCTGGATAG TAATGAAAGT ATTTCTCAAA TGAAAAACAT TGAGTCACTT 
GTGACTCTTA ATGCCAAAGT TGACTTTGAG TGCAGGACTC TTCCATTGAT AAGTCAGTCC 
CGCAGATTAG TACGAGAAGG GCCTGTGACT GAACTGAGAG ACTTTTCCTT GAAAGACAGA 
GAAGAAGAGA GAAGTGTTTA TATGCACCTT TTTAATGACT ACCTCTTGCT CTCTATACGG 
AAAGAAGGGG GTAGGTTTAC AGTTATCGAC CATGCTCCTG TATCAGAGCT GCGGGCCGAG 
AACTGCAGGT TCAAACTACA TTCCTTACAG AAAAACCTGT TCCGTCTGCA CATGCCACAA 
AAAGCCTTGC TTTTGCGAAC AGATACCCAA GCTGATAATC TGCGCTGGAT ATCAGCGCTC 
TCACGTCCTT ATCCTGAGAT TGACTTCGGT GCAGTTCAGG AAACTCCACA AATGCAGTGC 
ATCAGAGCAT ATGTTGCCCA GCAACCAGAT GAACTCAGTT TAGAGAAAGC TGATGTCCTT 
GTAGTTCACC AGCAAAGTAG TGATGGCTGG GTGGAAGGTA CTCGTTTGTC AGACAGGCAG 
CGTGGCTGGG CTCCTGAGCT TCACCTGGAG ACAATAACCA GTGACAAAGC AAGGAAGCGC 
AACCTGCTGG ATACCATGAA AATTGCTACT GCTGCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161540.2
CDS	773..4834
Translation	MDRERAMMQDSCDGTRERDRLPAIRRREMDWRERERWRDGDQYANGKSSLFEHTVHGERDNESKKGDTFPRMTKANRERDRRIPLSENERQRRLGRPKDTTYEDEKEREKRRYRDMDTRRHMKEHSDKLREQEFELGIRGRRREGHTGTNDPTGMREKETDRERDRYRQRDREVRYYPRPIERETESFSDKEWRTERQNGAERSRKRDTKSEGDSDGDRGKEMNREAVTKPGHKLYKSEGDSKADQRVDRVREKDRRRDQEAWRYKEMVRPREKDYRETGYYDKNGMRKDKRPDRDAYSNTPERGRYRNAELEKDGYSEREIEDRERWRRQNREPYRDKMEKSGQRTGGKISGVEIEKEHNRSVGENLEDRNRKNTSESEKEKLKGRTDGEQEVRIAPREEEGRQIVVEETVTKKFTGRIMNRWLEPRREKNPSLLEEDYRDRERARERYNQRFKESTKYEEDPARNNESKRTSAKDKYRAGEFMGDIDVESNEKLDKQEDMVNDKEAVEPEWRAKREIGGNNMEVNEREEERSIDRHDYSGIEEGSEGESRAEKDRIISADDGFVTVSSGGDIEDCMDFRNGEVPTDISGCSEATQGEEREPLRVFCVIGLTPIESQTKQNPYIDTASEEATDQHSDQNIDIQGVMFIEDANEAFEKFEHKESDIEGAMDDLALSANEEKVEQTGDNDQGTDDITSLKGQMIPSKEGAEEEPAEPSVTLDPCNDDGPETIVAPSEDALHRREMEATGNTEEHFDSDDTWSTCEERKRCSTAPHLTWAKNVVKEILGPNSEDTEHMRDAEEDELCLTWSEGDLRKLGRTKKRSSKYFNSQLYQEYSEVVQNREILLTNYDTTSLSGDNITIRSSSPLNHSPKLSHRPLPALPQVHLHPHSLSLNNSLKSLNLPQQSMNRPPSPRLSISASSPTLWQDLPGVRNSPEIVELTEDERRLQEVRFEVVTSEASYCRSLDIVVENFVLSKQLNAILSSQDKNWLFSRLNDVRAISHSFMSHLEENVERDMLHFTVCDIIIKYCPRFRNVYVPYLTNQSYQDKTYQRLMDESQEFRRVVEKLERNPVCQRLPLRSFLILPFQRITRLKLLVQNIVKRTAPKTKDEVQAIKAMKLLEKMILDSNESISQMKNIESLVTLNAKVDFECRTLPLISQSRRLVREGPVTELRDFSLKDREEERSVYMHLFNDYLLLSIRKEGGRFTVIDHAPVSELRAENCRFKLHSLQKNLFRLHMPQKALLLRTDTQADNLRWISALSRPYPEIDFGAVQETPQMQCIRAYVAQQPDELSLEKADVLVVHQQSSDGWVEGTRLSDRQRGWAPELHLETITSDKARKRNLLDTMKIATAAM

Target ORF information:

RefSeq Version	XM_005161483.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161483.4

ORF Insert Sequence:

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGCATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCTAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCAAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGATGCTGAG 
GAGGATGAAT TATGTTTGAC CTGGAGTGAA GGGGATCTAA GGAAACTTGG GAGGACAAAA 
AAGAGGAGTT CTAAATACTT TAACTCTCAA CTGTATCAGG AGTACAGTGA GGTCGTCCAG 
AACAGAGAAA TTCTGCTGAC TAATTATGAT ACTACATCGC TAAGTGGAGA TAATATCACC 
ATCCGTTCAT CTTCCCCTCT GAATCATTCG CCAAAGCTAT CTCACAGACC TCTCCCAGCG 
CTTCCTCAAG TCCACCTGCA TCCCCACTCA CTGTCCTTGA ATAATTCCCT AAAAAGTCTT 
AATTTACCTC AGCAATCTAT GAACAGACCC CCTTCTCCTC GCCTGTCTAT CTCCGCTTCC 
TCACCCACAC TGTGGCAGGA TCTCCCTGGA GTCAGGAACA GCCCAGAAAT TGTGGAACTT 
ACTGAAGATG AGAGACGCCT GCAGGAGGTG AGGTTTGAGG TTGTGACATC TGAGGCATCA 
TACTGTCGTA GTTTGGACAT CGTAGTGGAG AACTTTGTCC TGTCTAAACA GCTCAATGCT 
ATCCTGTCAT CACAAGACAA AAATTGGCTG TTCTCTAGGC TAAATGATGT TAGAGCCATC 
AGTCACAGTT TTATGTCTCA CCTTGAAGAA AACGTGGAAA GAGATATGTT GCACTTTACA 
GTCTGTGACA TCATTATTAA ATACTGTCCC CGATTCCGAA ATGTGTATGT GCCATATCTT 
ACCAACCAAT CATATCAGGA CAAGACGTAT CAAAGATTAA TGGATGAGAG TCAGGAATTT 
CGCAGAGTTG TGGAAAAGTT AGAGCGTAAT CCAGTGTGCC AGCGGTTGCC TTTACGGTCA 
TTTCTCATTC TTCCGTTCCA ACGAATTACA CGCCTCAAAC TATTAGTGCA GAATATTGTA 
AAGAGAACTG CTCCAAAAAC CAAAGATGAA GTGCAGGCCA TTAAGGCCAT GAAACTACTT 
GAGAAGATGA TCCTGGATAG TAATGAAAGT ATTTCTCAAA TGAAAAACAT TGAGTCACTT 
GTGACTCTTA ATGCCAAAGT TGACTTTGAG TGCAGGACTC TTCCATTGAT AAGTCAGTCC 
CGCAGATTAG TACGAGAAGG GCCTGTGACT GAACTGAGAG ACTTTTCCTT GAAAGACAGA 
GAAGAAGAGA GAAGTGTTTA TATGCACCTT TTTAATGACT ACCTCTTGCT CTCTATACGG 
AAAGAAGGGG GTAGGTTTAC AGTTATCGAC CATGCTCCTG TATCAGAGCT GCGGGCCGAG 
AACTGCAGGT TCAAACTACA TTCCTTACAG AAAAACCTGT TCCGTCTGCA CATGCCACAA 
AAAGCCTTGC TTTTGCGAAC AGATACCCAA GCTGATAATC TGCGCTGGAT ATCAGCGCTC 
TCACGTCCTT ATCCTGAGAT TGACTTCGGT GCAGTTCAGG AAACTCCACA AATGCAGTGC 
ATCAGAGCAT ATGTTGCCCA GCAACCAGAT GAACTCAGTT TAGAGAAAGC TGATGTCCTT 
GTAGTTCACC AGCAAAGTAG TGATGGCTGG GTGGAAGGTA CTCGTTTGTC AGACAGGCAG 
CGTGGCTGGG CTCCTGAGCT TCACCTGGAG ACAATAACCA GTGACAAAGC AAGGAAGCGC 
AACCTGCTGG ATACCATGAA AATTGCTACT GCTGCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef5 ( XM_003200169.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200217.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.

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CloneID	ODa21305
Clone ID Related Accession (Same CDS sequence)	XM_003200169.4
Accession Version	XM_003200169.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	zinc finger CCCH domain-containing protein 13-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200169.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGTATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCAAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCGAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGGTAGTGTG 
ACACACATAG ACATACAAAT AAACACACAA ACAGAAACTG ACAGAGAAAT TAACATATCG 
AGGGAACATG AAGAACTAAT ACAGGGAGAA GAGAAAGAAC AAGGAGAGAT TGAAAAGGAG 
TCAGATGAGG AAACTATCTT GTTGGATCCA AGTTGCATTG AACAGACAGT TTCCTCAAAA 
AGAGAGGCAG ATAGGACAGT AGAAGGACAC GTTTCTAAGA GGAAAGATAG CATAGAGAAT 
GTCATTCTTG GTTCAAGTAG CTTTCGAGAA TTGGGGAATG AGGCCCGCAT AAGGAGGAGG 
GGTATCCGAA AAACAACAGA GGGAACCAAA GAAGAGGAAG AGGATGAGGA AGAGGGAGGA 
GTTGGAAGGG ACCGCAGGAC CAGAGTATTC AACAATTCAG ATGCTGAGGA GGATGAATTA 
TGTTTGACCT GGAGTGAAGG GGATCTAAGG AAACTTGGGA GGACAAAAAA GAGGAGTTCT 
AAATACTTTA ACTCTCAACT GTATCAGGAG TACAGTGAGG TCGTCCAGAA CAGAGAAATT 
CTGCTGACTA ATTATGATAC TACATCGCTA AGTGGAGATA ATATCACCAT CCGTTCATCT 
TCCCCTCTGA ATCATTCGCC AAAGCTATCT CACAGACCTC TCCCAGCGCT TCCTCAAGTC 
CACCTGCATC CCCACTCACT GTCCTTGAAT AATTCCCTAA AAAGTCTTAA TTTACCTCAG 
CAATCTATGA ACAGACCCCC TTCTCCTCGC CTGTCTATCT CCGCTTCCTC ACCCACACTG 
TGGCAGGATC TCCCTGGAGT CAGGAACAGC CCAGAAATTG TGGAACTTAC TGAAGATGAG 
AGACGCCTGC AGGAGGTGAG GTTTGAGGTT GTGACATCTG AGGCATCATA CTGTCGTAGT 
TTGGACATCG TAGTGGAGAA CTTTGTCCTG TCTAAACAGC TCAATGCTAT CCTGTCATCA 
CAAGACAAAA ATTGGCTGTT CTCTAGGCTA AATGATGTTA GAGCCATCAG TCACAGTTTT 
ATGTCTCACC TTGAAGAAAA CGTGGAAAGA GATATGTTGC ACTTTACAGT CTGTGACATC 
ATTATTAAAT ACTGTCCCCG ATTCCGAAAT GTGTATGTGC CATATCTTAC CAACCAATCA 
TATCAGGACA AGACGTATCA AAGATTAATG GATGAGAGTC AGGAATTTCG CAGAGTTGTG 
GAAAAGTTAG AGCGTAATCC AGTGTGCCAG CGGTTGCCTT TACGGTCATT TCTCATTCTT 
CCGTTCCAAC GAATTACACG CCTCAAACTA TTAGTGCAGA ATATTGTAAA GAGAACTGCT 
CCAAAAACCA AAGATGAAGT GCAGGCCATT AAGGCCATGA AACTACTTGA GAAGATGATC 
CTGGATAGTA ATGAAAGTAT TTCTCAAATG AAAAACATTG AGTCACTTGT GACTCTTAAT 
GCCAAAGTTG ACTTTGAGTG CAGGACTCTT CCATTGATAA GTCAGTCCCG CAGATTAGTA 
CGAGAAGGGC CTGTGACTGA ACTGAGAGAC TTTTCCTTGA AAGACAGAGA AGAAGAGAGA 
AGTGTTTATA TGCACCTTTT TAATGACTAC CTCTTGCTCT CTATACGGAA AGAAGGGGGT 
AGGTTTACAG TTATCGACCA TGCTCCTGTA TCAGAGCTGC GGGCCGAGAA CTGCAGGTTC 
AAACTACATT CCTTACAGAA AAACCTGTTC CGTCTGCACA TGCCACAAAA AGCCTTGCTT 
TTGCGAACAG ATACCCAAGC TGATAATCTG CGCTGGATAT CAGCGCTCTC ACGTCCTTAT 
CCTGAGATTG ACTTCGGTGC AGTTCAGGAA ACTCCACAAA TGCAGTGCAT CAGAGCATAT 
GTTGCCCAGC AACCAGATGA ACTCAGTTTA GAGAAAGCTG ATGTCCTTGT AGTTCACCAG 
CAAAGTAGTG ATGGCTGGGT GGAAGGTACT CGTTTGTCAG ACAGGCAGCG TGGCTGGGCT 
CCTGAGCTTC ACCTGGAGAC AATAACCAGT GACAAAGCAA GGAAGCGCAA CCTGCTGGAT 
ACCATGAAAA TTGCTACTGC TGCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200217.2
CDS	663..5132
Translation	MDRERAMMQDSCDGTRERDRLPAIRRREMDWRERERWRDGDQYANGKSSLFEHTVHGERDNESKKGDTFPRMTKANRERDRRIPLSENERQRRLGRPKDTTYEDEKEREKRRYRDMDTRRHMKEHSDKLREQEFELGIRGRRREGHTGTNDPTGMREKETDRERDRYRQRDREVRYYPRPIERETESFSDKEWRTERQNGAERSRKRDTKSEGDSDGDRGKEMNREAVTKPGHKLYKSEGDSKADQRVDRVREKDRRRDQEVWRYKEMVRPREKDYRETGYYDKNGMRKDKRPDRDAYSNTPERGRYRNAELEKDGYSEREIEDRERWRRQNREPYRDKMEKSGQRTGGKISGVEIEKEHNRSVGENLEDRNRKNTSESEKEKLKGRTDGEQEVRIAPREEEGRQIVVEETVTKKFTGRIMNRWLEPRREKNPSLLEEDYRDRERARERYNQRFKESTKYEEDPARNNESKRTSAKDKYRAGEFMGDIDVESNEKLDKQEDMVNDKEAVEPEWRAKREIGGNNMEVNEREEERSIDRHDYSGIEEGSEGESRAEKDRIISADDGFVTVSSGGDIEDCMDFRNGEVPTDISGCSEATQGEEREPLRVFCVIGQTPIESQTKQNPYIDTASEEATDQHSDQNIDIQGVMFIEDANEAFEKFEHKESDIEGAMDDLALSANEEKVEQTGDNDQGTDDITSLKGQMIPSKEGAEEEPAEPSVTLDPCNDDGPETIVAPSEDALHRREMEATGNTEEHFDSDDTWSTCEERKRCSTAPHLTWAKNVVKEILGPNSEDTEHMRGSVTHIDIQINTQTETDREINISREHEELIQGEEKEQGEIEKESDEETILLDPSCIEQTVSSKREADRTVEGHVSKRKDSIENVILGSSSFRELGNEARIRRRGIRKTTEGTKEEEEDEEEGGVGRDRRTRVFNNSDAEEDELCLTWSEGDLRKLGRTKKRSSKYFNSQLYQEYSEVVQNREILLTNYDTTSLSGDNITIRSSSPLNHSPKLSHRPLPALPQVHLHPHSLSLNNSLKSLNLPQQSMNRPPSPRLSISASSPTLWQDLPGVRNSPEIVELTEDERRLQEVRFEVVTSEASYCRSLDIVVENFVLSKQLNAILSSQDKNWLFSRLNDVRAISHSFMSHLEENVERDMLHFTVCDIIIKYCPRFRNVYVPYLTNQSYQDKTYQRLMDESQEFRRVVEKLERNPVCQRLPLRSFLILPFQRITRLKLLVQNIVKRTAPKTKDEVQAIKAMKLLEKMILDSNESISQMKNIESLVTLNAKVDFECRTLPLISQSRRLVREGPVTELRDFSLKDREEERSVYMHLFNDYLLLSIRKEGGRFTVIDHAPVSELRAENCRFKLHSLQKNLFRLHMPQKALLLRTDTQADNLRWISALSRPYPEIDFGAVQETPQMQCIRAYVAQQPDELSLEKADVLVVHQQSSDGWVEGTRLSDRQRGWAPELHLETITSDKARKRNLLDTMKIATAAM

Target ORF information:

RefSeq Version	XM_003200169.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200169.4

ORF Insert Sequence:

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGTATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCAAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCGAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGGTAGTGTG 
ACACACATAG ACATACAAAT AAACACACAA ACAGAAACTG ACAGAGAAAT TAACATATCG 
AGGGAACATG AAGAACTAAT ACAGGGAGAA GAGAAAGAAC AAGGAGAGAT TGAAAAGGAG 
TCAGATGAGG AAACTATCTT GTTGGATCCA AGTTGCATTG AACAGACAGT TTCCTCAAAA 
AGAGAGGCAG ATAGGACAGT AGAAGGACAC GTTTCTAAGA GGAAAGATAG CATAGAGAAT 
GTCATTCTTG GTTCAAGTAG CTTTCGAGAA TTGGGGAATG AGGCCCGCAT AAGGAGGAGG 
GGTATCCGAA AAACAACAGA GGGAACCAAA GAAGAGGAAG AGGATGAGGA AGAGGGAGGA 
GTTGGAAGGG ACCGCAGGAC CAGAGTATTC AACAATTCAG ATGCTGAGGA GGATGAATTA 
TGTTTGACCT GGAGTGAAGG GGATCTAAGG AAACTTGGGA GGACAAAAAA GAGGAGTTCT 
AAATACTTTA ACTCTCAACT GTATCAGGAG TACAGTGAGG TCGTCCAGAA CAGAGAAATT 
CTGCTGACTA ATTATGATAC TACATCGCTA AGTGGAGATA ATATCACCAT CCGTTCATCT 
TCCCCTCTGA ATCATTCGCC AAAGCTATCT CACAGACCTC TCCCAGCGCT TCCTCAAGTC 
CACCTGCATC CCCACTCACT GTCCTTGAAT AATTCCCTAA AAAGTCTTAA TTTACCTCAG 
CAATCTATGA ACAGACCCCC TTCTCCTCGC CTGTCTATCT CCGCTTCCTC ACCCACACTG 
TGGCAGGATC TCCCTGGAGT CAGGAACAGC CCAGAAATTG TGGAACTTAC TGAAGATGAG 
AGACGCCTGC AGGAGGTGAG GTTTGAGGTT GTGACATCTG AGGCATCATA CTGTCGTAGT 
TTGGACATCG TAGTGGAGAA CTTTGTCCTG TCTAAACAGC TCAATGCTAT CCTGTCATCA 
CAAGACAAAA ATTGGCTGTT CTCTAGGCTA AATGATGTTA GAGCCATCAG TCACAGTTTT 
ATGTCTCACC TTGAAGAAAA CGTGGAAAGA GATATGTTGC ACTTTACAGT CTGTGACATC 
ATTATTAAAT ACTGTCCCCG ATTCCGAAAT GTGTATGTGC CATATCTTAC CAACCAATCA 
TATCAGGACA AGACGTATCA AAGATTAATG GATGAGAGTC AGGAATTTCG CAGAGTTGTG 
GAAAAGTTAG AGCGTAATCC AGTGTGCCAG CGGTTGCCTT TACGGTCATT TCTCATTCTT 
CCGTTCCAAC GAATTACACG CCTCAAACTA TTAGTGCAGA ATATTGTAAA GAGAACTGCT 
CCAAAAACCA AAGATGAAGT GCAGGCCATT AAGGCCATGA AACTACTTGA GAAGATGATC 
CTGGATAGTA ATGAAAGTAT TTCTCAAATG AAAAACATTG AGTCACTTGT GACTCTTAAT 
GCCAAAGTTG ACTTTGAGTG CAGGACTCTT CCATTGATAA GTCAGTCCCG CAGATTAGTA 
CGAGAAGGGC CTGTGACTGA ACTGAGAGAC TTTTCCTTGA AAGACAGAGA AGAAGAGAGA 
AGTGTTTATA TGCACCTTTT TAATGACTAC CTCTTGCTCT CTATACGGAA AGAAGGGGGT 
AGGTTTACAG TTATCGACCA TGCTCCTGTA TCAGAGCTGC GGGCCGAGAA CTGCAGGTTC 
AAACTACATT CCTTACAGAA AAACCTGTTC CGTCTGCACA TGCCACAAAA AGCCTTGCTT 
TTGCGAACAG ATACCCAAGC TGATAATCTG CGCTGGATAT CAGCGCTCTC ACGTCCTTAT 
CCTGAGATTG ACTTCGGTGC AGTTCAGGAA ACTCCACAAA TGCAGTGCAT CAGAGCATAT 
GTTGCCCAGC AACCAGATGA ACTCAGTTTA GAGAAAGCTG ATGTCCTTGT AGTTCACCAG 
CAAAGTAGTG ATGGCTGGGT GGAAGGTACT CGTTTGTCAG ACAGGCAGCG TGGCTGGGCT 
CCTGAGCTTC ACCTGGAGAC AATAACCAGT GACAAAGCAA GGAAGCGCAA CCTGCTGGAT 
ACCATGAAAA TTGCTACTGC TGCAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef5 ( XM_005161483.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161540.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.

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CloneID	ODa21306
Clone ID Related Accession (Same CDS sequence)	XM_005161483.3
Accession Version	XM_005161483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein arhgef5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGTATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCAAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCGAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGATGCTGAG 
GAGGATGAAT TATGTTTGAC CTGGAGTGAA GGGGATCTAA GGAAACTTGG GAGGACAAAA 
AAGAGGAGTT CTAAATACTT TAACTCTCAA CTGTATCAGG AGTACAGTGA GGTCGTCCAG 
AACAGAGAAA TTCTGCTGAC TAATTATGAT ACTACATCGC TAAGTGGAGA TAATATCACC 
ATCCGTTCAT CTTCCCCTCT GAATCATTCG CCAAAGCTAT CTCACAGACC TCTCCCAGCG 
CTTCCTCAAG TCCACCTGCA TCCCCACTCA CTGTCCTTGA ATAATTCCCT AAAAAGTCTT 
AATTTACCTC AGCAATCTAT GAACAGACCC CCTTCTCCTC GCCTGTCTAT CTCCGCTTCC 
TCACCCACAC TGTGGCAGGA TCTCCCTGGA GTCAGGAACA GCCCAGAAAT TGTGGAACTT 
ACTGAAGATG AGAGACGCCT GCAGGAGGTG AGGTTTGAGG TTGTGACATC TGAGGCATCA 
TACTGTCGTA GTTTGGACAT CGTAGTGGAG AACTTTGTCC TGTCTAAACA GCTCAATGCT 
ATCCTGTCAT CACAAGACAA AAATTGGCTG TTCTCTAGGC TAAATGATGT TAGAGCCATC 
AGTCACAGTT TTATGTCTCA CCTTGAAGAA AACGTGGAAA GAGATATGTT GCACTTTACA 
GTCTGTGACA TCATTATTAA ATACTGTCCC CGATTCCGAA ATGTGTATGT GCCATATCTT 
ACCAACCAAT CATATCAGGA CAAGACGTAT CAAAGATTAA TGGATGAGAG TCAGGAATTT 
CGCAGAGTTG TGGAAAAGTT AGAGCGTAAT CCAGTGTGCC AGCGGTTGCC TTTACGGTCA 
TTTCTCATTC TTCCGTTCCA ACGAATTACA CGCCTCAAAC TATTAGTGCA GAATATTGTA 
AAGAGAACTG CTCCAAAAAC CAAAGATGAA GTGCAGGCCA TTAAGGCCAT GAAACTACTT 
GAGAAGATGA TCCTGGATAG TAATGAAAGT ATTTCTCAAA TGAAAAACAT TGAGTCACTT 
GTGACTCTTA ATGCCAAAGT TGACTTTGAG TGCAGGACTC TTCCATTGAT AAGTCAGTCC 
CGCAGATTAG TACGAGAAGG GCCTGTGACT GAACTGAGAG ACTTTTCCTT GAAAGACAGA 
GAAGAAGAGA GAAGTGTTTA TATGCACCTT TTTAATGACT ACCTCTTGCT CTCTATACGG 
AAAGAAGGGG GTAGGTTTAC AGTTATCGAC CATGCTCCTG TATCAGAGCT GCGGGCCGAG 
AACTGCAGGT TCAAACTACA TTCCTTACAG AAAAACCTGT TCCGTCTGCA CATGCCACAA 
AAAGCCTTGC TTTTGCGAAC AGATACCCAA GCTGATAATC TGCGCTGGAT ATCAGCGCTC 
TCACGTCCTT ATCCTGAGAT TGACTTCGGT GCAGTTCAGG AAACTCCACA AATGCAGTGC 
ATCAGAGCAT ATGTTGCCCA GCAACCAGAT GAACTCAGTT TAGAGAAAGC TGATGTCCTT 
GTAGTTCACC AGCAAAGTAG TGATGGCTGG GTGGAAGGTA CTCGTTTGTC AGACAGGCAG 
CGTGGCTGGG CTCCTGAGCT TCACCTGGAG ACAATAACCA GTGACAAAGC AAGGAAGCGC 
AACCTGCTGG ATACCATGAA AATTGCTACT GCTGCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161540.1
CDS	662..4723
Translation	MDRERAMMQDSCDGTRERDRLPAIRRREMDWRERERWRDGDQYANGKSSLFEHTVHGERDNESKKGDTFPRMTKANRERDRRIPLSENERQRRLGRPKDTTYEDEKEREKRRYRDMDTRRHMKEHSDKLREQEFELGIRGRRREGHTGTNDPTGMREKETDRERDRYRQRDREVRYYPRPIERETESFSDKEWRTERQNGAERSRKRDTKSEGDSDGDRGKEMNREAVTKPGHKLYKSEGDSKADQRVDRVREKDRRRDQEVWRYKEMVRPREKDYRETGYYDKNGMRKDKRPDRDAYSNTPERGRYRNAELEKDGYSEREIEDRERWRRQNREPYRDKMEKSGQRTGGKISGVEIEKEHNRSVGENLEDRNRKNTSESEKEKLKGRTDGEQEVRIAPREEEGRQIVVEETVTKKFTGRIMNRWLEPRREKNPSLLEEDYRDRERARERYNQRFKESTKYEEDPARNNESKRTSAKDKYRAGEFMGDIDVESNEKLDKQEDMVNDKEAVEPEWRAKREIGGNNMEVNEREEERSIDRHDYSGIEEGSEGESRAEKDRIISADDGFVTVSSGGDIEDCMDFRNGEVPTDISGCSEATQGEEREPLRVFCVIGQTPIESQTKQNPYIDTASEEATDQHSDQNIDIQGVMFIEDANEAFEKFEHKESDIEGAMDDLALSANEEKVEQTGDNDQGTDDITSLKGQMIPSKEGAEEEPAEPSVTLDPCNDDGPETIVAPSEDALHRREMEATGNTEEHFDSDDTWSTCEERKRCSTAPHLTWAKNVVKEILGPNSEDTEHMRDAEEDELCLTWSEGDLRKLGRTKKRSSKYFNSQLYQEYSEVVQNREILLTNYDTTSLSGDNITIRSSSPLNHSPKLSHRPLPALPQVHLHPHSLSLNNSLKSLNLPQQSMNRPPSPRLSISASSPTLWQDLPGVRNSPEIVELTEDERRLQEVRFEVVTSEASYCRSLDIVVENFVLSKQLNAILSSQDKNWLFSRLNDVRAISHSFMSHLEENVERDMLHFTVCDIIIKYCPRFRNVYVPYLTNQSYQDKTYQRLMDESQEFRRVVEKLERNPVCQRLPLRSFLILPFQRITRLKLLVQNIVKRTAPKTKDEVQAIKAMKLLEKMILDSNESISQMKNIESLVTLNAKVDFECRTLPLISQSRRLVREGPVTELRDFSLKDREEERSVYMHLFNDYLLLSIRKEGGRFTVIDHAPVSELRAENCRFKLHSLQKNLFRLHMPQKALLLRTDTQADNLRWISALSRPYPEIDFGAVQETPQMQCIRAYVAQQPDELSLEKADVLVVHQQSSDGWVEGTRLSDRQRGWAPELHLETITSDKARKRNLLDTMKIATAAM

Target ORF information:

RefSeq Version	XM_005161483.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho guanine nucleotide exchange factor (GEF) 5 (arhgef5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161483.3

ORF Insert Sequence:

ATGGACAGAG AAAGAGCAAT GATGCAAGAT TCATGTGATG GGACTAGGGA GAGGGACAGA 
CTCCCTGCAA TTAGAAGAAG AGAAATGGAC TGGAGAGAAA GAGAGAGATG GAGAGATGGA 
GATCAATATG CAAATGGGAA GTCATCATTG TTTGAGCACA CTGTACATGG AGAACGAGAC 
AACGAAAGCA AAAAAGGGGA CACTTTTCCG AGGATGACAA AAGCAAACAG AGAGAGAGAT 
AGAAGAATTC CACTTTCAGA GAATGAAAGG CAACGTCGTT TGGGAAGACC TAAGGACACA 
ACCTATGAGG ATGAAAAAGA GAGGGAAAAA CGAAGATACA GAGATATGGA CACAAGAAGA 
CACATGAAGG AGCATTCAGA TAAACTAAGA GAACAAGAGT TTGAATTAGG AATAAGAGGG 
AGAAGGAGAG AAGGTCATAC AGGGACCAAT GACCCTACAG GAATGAGGGA AAAAGAGACA 
GACAGAGAGA GAGACAGATA TAGACAAAGA GACAGAGAGG TCAGGTATTA TCCACGACCC 
ATAGAAAGAG AGACAGAGAG TTTTTCAGAC AAGGAATGGA GGACTGAGAG ACAGAATGGG 
GCAGAAAGAT CTAGGAAAAG AGATACAAAA AGTGAAGGGG ACAGTGATGG AGATAGAGGT 
AAGGAAATGA ATAGAGAAGC GGTTACTAAA CCTGGACACA AACTCTATAA GAGTGAAGGA 
GATAGTAAAG CAGACCAAAG AGTAGACCGA GTTAGAGAAA AAGACAGACG TAGAGACCAA 
GAAGTATGGA GATATAAAGA AATGGTGAGA CCTAGAGAAA AAGACTACAG GGAGACAGGC 
TACTATGATA AAAATGGAAT GAGAAAAGAT AAAAGGCCTG ACAGAGATGC ATACTCAAAT 
ACACCTGAGA GAGGAAGATA CAGAAATGCA GAATTGGAGA AGGATGGATA TTCTGAAAGA 
GAGATTGAAG ACAGGGAGAG GTGGAGAAGA CAGAACAGAG AGCCATATCG AGACAAGATG 
GAGAAAAGTG GACAACGTAC TGGAGGGAAG ATTTCAGGAG TTGAAATTGA GAAAGAACAT 
AATAGGTCAG TGGGGGAGAA CCTTGAGGAC AGGAATAGAA AAAACACTTC TGAGAGTGAA 
AAGGAGAAGT TGAAAGGAAG AACAGATGGA GAGCAGGAAG TACGGATTGC ACCAAGGGAA 
GAAGAGGGTA GACAAATTGT TGTTGAGGAA ACAGTTACAA AAAAATTTAC AGGCAGAATA 
ATGAACAGGT GGCTGGAACC CCGAAGAGAG AAAAACCCAT CTCTTTTAGA GGAGGACTAC 
AGAGACAGAG AAAGAGCACG GGAGAGGTAC AACCAAAGAT TCAAGGAAAG TACGAAGTAT 
GAAGAAGATC CTGCAAGAAA TAATGAAAGC AAGAGAACGA GTGCTAAGGA CAAGTATAGG 
GCTGGGGAAT TCATGGGGGA CATAGATGTA GAAAGCAATG AAAAACTAGA TAAACAAGAG 
GACATGGTTA ATGACAAAGA AGCCGTGGAA CCAGAATGGC GGGCAAAAAG AGAAATTGGG 
GGTAATAACA TGGAAGTTAA TGAAAGAGAG GAAGAGAGAA GCATTGATCG ACACGACTAC 
TCAGGAATTG AGGAAGGAAG TGAGGGAGAA TCAAGAGCAG AGAAAGACAG AATAATATCA 
GCAGATGATG GATTTGTGAC AGTCTCTAGT GGAGGAGATA TTGAAGACTG TATGGATTTC 
CGGAATGGTG AAGTTCCCAC TGATATCTCT GGATGCTCAG AAGCTACACA GGGTGAAGAG 
AGAGAACCCC TTAGGGTTTT TTGTGTGATT GGTCAAACCC CTATTGAGTC ACAAACCAAG 
CAGAATCCAT ATATAGATAC TGCTTCTGAG GAAGCCACCG ATCAGCATTC AGATCAAAAC 
ATAGACATCC AAGGTGTTAT GTTTATTGAG GATGCAAATG AAGCATTTGA AAAGTTTGAA 
CACAAGGAAT CTGACATAGA AGGAGCAATG GATGACCTCG CTCTTTCTGC TAATGAGGAA 
AAAGTGGAGC AAACAGGAGA TAATGACCAG GGAACAGATG ACATAACATC ACTGAAAGGT 
CAAATGATTC CTTCCAAAGA GGGAGCAGAA GAAGAGCCTG CAGAGCCTTC TGTAACTTTA 
GACCCCTGCA ATGATGATGG ACCTGAGACT ATAGTGGCTC CTAGTGAAGA TGCATTACAC 
AGAAGAGAAA TGGAAGCTAC TGGAAACACA GAGGAGCATT TTGATTCAGA TGACACATGG 
AGCACATGTG AAGAACGAAA GCGATGCTCT ACTGCTCCTC ACCTTACATG GGCGAAGAAT 
GTTGTGAAAG AGATCCTGGG TCCGAATTCA GAAGACACAG AACATATGAG AGATGCTGAG 
GAGGATGAAT TATGTTTGAC CTGGAGTGAA GGGGATCTAA GGAAACTTGG GAGGACAAAA 
AAGAGGAGTT CTAAATACTT TAACTCTCAA CTGTATCAGG AGTACAGTGA GGTCGTCCAG 
AACAGAGAAA TTCTGCTGAC TAATTATGAT ACTACATCGC TAAGTGGAGA TAATATCACC 
ATCCGTTCAT CTTCCCCTCT GAATCATTCG CCAAAGCTAT CTCACAGACC TCTCCCAGCG 
CTTCCTCAAG TCCACCTGCA TCCCCACTCA CTGTCCTTGA ATAATTCCCT AAAAAGTCTT 
AATTTACCTC AGCAATCTAT GAACAGACCC CCTTCTCCTC GCCTGTCTAT CTCCGCTTCC 
TCACCCACAC TGTGGCAGGA TCTCCCTGGA GTCAGGAACA GCCCAGAAAT TGTGGAACTT 
ACTGAAGATG AGAGACGCCT GCAGGAGGTG AGGTTTGAGG TTGTGACATC TGAGGCATCA 
TACTGTCGTA GTTTGGACAT CGTAGTGGAG AACTTTGTCC TGTCTAAACA GCTCAATGCT 
ATCCTGTCAT CACAAGACAA AAATTGGCTG TTCTCTAGGC TAAATGATGT TAGAGCCATC 
AGTCACAGTT TTATGTCTCA CCTTGAAGAA AACGTGGAAA GAGATATGTT GCACTTTACA 
GTCTGTGACA TCATTATTAA ATACTGTCCC CGATTCCGAA ATGTGTATGT GCCATATCTT 
ACCAACCAAT CATATCAGGA CAAGACGTAT CAAAGATTAA TGGATGAGAG TCAGGAATTT 
CGCAGAGTTG TGGAAAAGTT AGAGCGTAAT CCAGTGTGCC AGCGGTTGCC TTTACGGTCA 
TTTCTCATTC TTCCGTTCCA ACGAATTACA CGCCTCAAAC TATTAGTGCA GAATATTGTA 
AAGAGAACTG CTCCAAAAAC CAAAGATGAA GTGCAGGCCA TTAAGGCCAT GAAACTACTT 
GAGAAGATGA TCCTGGATAG TAATGAAAGT ATTTCTCAAA TGAAAAACAT TGAGTCACTT 
GTGACTCTTA ATGCCAAAGT TGACTTTGAG TGCAGGACTC TTCCATTGAT AAGTCAGTCC 
CGCAGATTAG TACGAGAAGG GCCTGTGACT GAACTGAGAG ACTTTTCCTT GAAAGACAGA 
GAAGAAGAGA GAAGTGTTTA TATGCACCTT TTTAATGACT ACCTCTTGCT CTCTATACGG 
AAAGAAGGGG GTAGGTTTAC AGTTATCGAC CATGCTCCTG TATCAGAGCT GCGGGCCGAG 
AACTGCAGGT TCAAACTACA TTCCTTACAG AAAAACCTGT TCCGTCTGCA CATGCCACAA 
AAAGCCTTGC TTTTGCGAAC AGATACCCAA GCTGATAATC TGCGCTGGAT ATCAGCGCTC 
TCACGTCCTT ATCCTGAGAT TGACTTCGGT GCAGTTCAGG AAACTCCACA AATGCAGTGC 
ATCAGAGCAT ATGTTGCCCA GCAACCAGAT GAACTCAGTT TAGAGAAAGC TGATGTCCTT 
GTAGTTCACC AGCAAAGTAG TGATGGCTGG GTGGAAGGTA CTCGTTTGTC AGACAGGCAG 
CGTGGCTGGG CTCCTGAGCT TCACCTGGAG ACAATAACCA GTGACAAAGC AAGGAAGCGC 
AACCTGCTGG ATACCATGAA AATTGCTACT GCTGCAATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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arhgef5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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