chata cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chata
Entrez Gene ID	100170938
Full Name	choline O-acetyltransferase a
Synonyms	chat
General protein information	Preferred Names choline O-acetyltransferase a Names choline O-acetyltransferase CHOACTase baj choline acetylase choline acetyltransferase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001130719.1	NP_001124191.1	choline O-acetyltransferase
XM_005156729.4	XP_005156786.1	choline O-acetyltransferase isoform X1
XM_021480489.1	XP_021336164.1	choline O-acetyltransferase isoform X2
XM_005156729.3	XP_005156786.1	choline O-acetyltransferase isoform X1
XM_021480488.1	XP_021336163.1	choline O-acetyltransferase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chat	NP_034021.1
Drosophila melanogaster (fruit fly)	Cha	NP_477004.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002370	XP_312586.5
Caenorhabditis elegans (roundworm)	cha-1	NP_001023603.1
Homo sapiens (human)	CHAT	NP_001136405.1
Pan troglodytes (chimpanzee)	CHAT	XP_003339420.1
Xenopus tropicalis (tropical clawed frog)	chat	XP_002933959.1
Macaca mulatta (Rhesus monkey)	CHAT	XP_002805679.1
Bos taurus (cattle)	CHAT	XP_002698999.2
Canis lupus familiaris (dog)	CHAT	XP_005637542.1
Rattus norvegicus (Norway rat)	Chat	NP_001164064.1
Gallus gallus (chicken)	CHAT	NP_989941.1
Danio rerio (zebrafish)	chat	NP_001124191.1

Gene Ontology
Bibliography

Related articles in PubMed

Nucleus Isthmi Is Required to Sustain Target Pursuit during Visually Guided Prey-Catching.
Henriques PM, Rahman N, Jackson SE, Bianco IH
Current biology : CB29(11)1771-1786.e5(2019 Jun)

Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene.
Nikaido M, Izumi S, Ohnuki H, Takigawa Y, Yamasu K, Hatta K
Gene expression patterns : GEP2812-21(2018 06)

Mutation of a serine near the catalytic site of the choline acetyltransferase a gene almost completely abolishes motility of the zebrafish embryo.
Joshi S, Virdi S, Etard C, Geisler R, Strähle U
PloS one13(11)e0207747(2018)

Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health.
Heid J, Cencioni C, Ripa R, Baumgart M, Atlante S, Milano G, Scopece A, Kuenne C, Guenther S, Azzimato V, Farsetti A, Rossi G, Braun T, Pompilio G, Martelli F, Zeiher AM, Cellerino A, Gaetano C, Spallotta F
Scientific reports7(1)16839(2017 12)

Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.
Förster D, Arnold-Ammer I, Laurell E, Barker AJ, Fernandes AM, Finger-Baier K, Filosa A, Helmbrecht TO, Kölsch Y, Kühn E, Robles E, Slanchev K, Thiele TR, Baier H, Kubo F
Scientific reports7(1)5230(2017 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chata gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chata cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148461	XM_021480489.1 Latest version!	Danio rerio choline O-acetyltransferase a (chata), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODa01581	NM_001130719.1 Latest version!	Danio rerio choline O-acetyltransferase a (chata), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa18688	XM_021480488.1 Latest version!	Danio rerio choline O-acetyltransferase a (chata), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa18688	XM_005156729.4 Latest version!	Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ODa18688	XM_005156729.3	Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chata ( XM_021480489.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336164.1 Danio rerio choline O-acetyltransferase a (chata), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148461
Clone ID Related Accession (Same CDS sequence)	XM_021480489.1
Accession Version	XM_021480489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	choline O-acetyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336164.1
CDS	584..1759
Translation	MPVSKREQSKDTGDPCALPKLPIPPLKQTLDMYLTCMGHLVPEDQFRKTKAVVEKFGAPGGVGETLQKKLLERSEQKANWVYDYWLEDMYLNNRLALPVNSSPVMVFHKQNFKGQSDVLRFAANLISGVLEYKALIDGRALPVEHARGQLAGTPLCMDQYNKVFTSYRLPGTKTDTLVAQKSTVMPEPEHIIVACKNQFFVLDVMVNFRRLNEKDLYTQLERIRKMADIEEERQPPIGLLTSDGRTQWAEARNILIKDSTNRDSLDMIERCLCLVCLDEETATELNDSNRALLMLHGGGTDKNGGNRWYDKPMQFVIGADGCCGVVCEHSPFEGIVLVQCSEYLLRYMRGSPSKLVRAASMSELPAPRRLRWKCSPDIQTFLSASADRLQK

Target ORF information:

RefSeq Version	XM_021480489.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline O-acetyltransferase a (chata), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480489.1

ORF Insert Sequence:

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chata ( NM_001130719.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001124191.1 Danio rerio choline O-acetyltransferase a (chata), mRNA.

Price & Availability↑

CloneID	ODa01581
Clone ID Related Accession (Same CDS sequence)	NM_001130719.1
Accession Version	NM_001130719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572624000000
Organism	Danio rerio(zebrafish)
Product	choline O-acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU660883.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU660883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCG GGAACAAAGA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124191.1
CDS	1..1914
Translation	MPVSKREQSKDTGDPCALPKLPIPPLKQTLDMYLTCMGHLVPEDQFRKTKAVVEKFGAPGGVGETLQKKLLERSEQKANWVYDYWLEDMYLNNRLALPVNSSPVMVFHKQNFKGQSDVLRFAANLISGVLEYKALIDGRALPVEHARGQLAGTPLCMDQYNKVFTSYRLPGTKTDTLVAQKSTVMPEPEHIIVACKNQFFVLDVMVNFRRLNEKDLYTQLERIRKMADIEEERQPPIGLLTSDGRTQWAEARNILIKDSTNRDSLDMIERCLCLVCLDEETATELNDSNRALLMLHGGGTDKNGGNRWYDKPMQFVIGADGCCGVVCEHSPFEGIVLVQCSEYLLRYMRGSPSKLVRAASMSELPAPRRLRWKCSPDIQTFLSASADRLQKLVKNLDMNVHKFTGYGKEFIKRQKMSPDAYVQVALQFTFYRCHGRLVPTYESASIRRFQEGRVDNIRSSTPEALAFVKAMASGSKITDAEKMELLWTAIKAQTNYTILAITGMAIDNHLLGLREIAKELKLEKPELFSDTTYATSIHFTLSTSQVPTTEEMFCCYGPVVPNGYGACYNPQTDHILFCVSSFRECAETSSDLFVKTLEGCLKEMQDLCRKCNTEVKPADSTQRMEGNPKVMKNGSKS

Target ORF information:

RefSeq Version	NM_001130719.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline O-acetyltransferase a (chata), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130719.1

ORF Insert Sequence:

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCG GGAACAAAGA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chata ( XM_005156729.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156786.1 Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18688
Clone ID Related Accession (Same CDS sequence)	XM_005156729.4 , XM_005156729.3 , XM_021480488.1
Accession Version	XM_005156729.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156729.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156786.1
CDS	548..2461
Translation	MPVSKREQSKDTGDPCALPKLPIPPLKQTLDMYLTCMGHLVPEDQFRKTKAVVEKFGAPGGVGETLQKKLLERSEQKANWVYDYWLEDMYLNNRLALPVNSSPVMVFHKQNFKGQSDVLRFAANLISGVLEYKALIDGRALPVEHARGQLAGTPLCMDQYNKVFTSYRLPGTKTDTLVAQKSTVMPEPEHIIVACKNQFFVLDVMVNFRRLNEKDLYTQLERIRKMADIEEERQPPIGLLTSDGRTQWAEARNILIKDSTNRDSLDMIERCLCLVCLDEETATELNDSNRALLMLHGGGTDKNGGNRWYDKPMQFVIGADGCCGVVCEHSPFEGIVLVQCSEYLLRYMRGSPSKLVRAASMSELPAPRRLRWKCSPDIQTFLSASADRLQKLVKNLDMNVHKFTGYGKEFIKRQKMSPDAYVQVALQFTFYRCHGRLVPTYESASIRRFQEGRVDNIRSSTPEALAFVKAMASGSKITDAEKMELLWTAIKAQTNYTILAITGMAIDNHLLGLREIAKELKLEKPELFSDTTYATSIHFTLSTSQVPTTEEMFCCYGPVVPNGYGACYNPQTDHILFCVSSFRECAETSSDLFVKTLEGCLKEMQDLCRKCNTEVKPADSTQRMEGNPKVMKNGSKS

Target ORF information:

RefSeq Version	XM_005156729.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156729.4

ORF Insert Sequence:

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chata ( XM_005156729.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156786.1 Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18688
Clone ID Related Accession (Same CDS sequence)	XM_005156729.4 , XM_005156729.3 , XM_021480488.1
Accession Version	XM_005156729.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	choline O-acetyltransferase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156786.1
CDS	380..2293
Translation	MPVSKREQSKDTGDPCALPKLPIPPLKQTLDMYLTCMGHLVPEDQFRKTKAVVEKFGAPGGVGETLQKKLLERSEQKANWVYDYWLEDMYLNNRLALPVNSSPVMVFHKQNFKGQSDVLRFAANLISGVLEYKALIDGRALPVEHARGQLAGTPLCMDQYNKVFTSYRLPGTKTDTLVAQKSTVMPEPEHIIVACKNQFFVLDVMVNFRRLNEKDLYTQLERIRKMADIEEERQPPIGLLTSDGRTQWAEARNILIKDSTNRDSLDMIERCLCLVCLDEETATELNDSNRALLMLHGGGTDKNGGNRWYDKPMQFVIGADGCCGVVCEHSPFEGIVLVQCSEYLLRYMRGSPSKLVRAASMSELPAPRRLRWKCSPDIQTFLSASADRLQKLVKNLDMNVHKFTGYGKEFIKRQKMSPDAYVQVALQFTFYRCHGRLVPTYESASIRRFQEGRVDNIRSSTPEALAFVKAMASGSKITDAEKMELLWTAIKAQTNYTILAITGMAIDNHLLGLREIAKELKLEKPELFSDTTYATSIHFTLSTSQVPTTEEMFCCYGPVVPNGYGACYNPQTDHILFCVSSFRECAETSSDLFVKTLEGCLKEMQDLCRKCNTEVKPADSTQRMEGNPKVMKNGSKS

Target ORF information:

RefSeq Version	XM_005156729.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio choline O-acetyltransferase a (chata), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156729.3

ORF Insert Sequence:

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chata ( XM_021480488.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336163.1 Danio rerio choline O-acetyltransferase a (chata), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18688
Clone ID Related Accession (Same CDS sequence)	XM_005156729.4 , XM_005156729.3 , XM_021480488.1
Accession Version	XM_021480488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	choline O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336163.1
CDS	590..2503
Translation	MPVSKREQSKDTGDPCALPKLPIPPLKQTLDMYLTCMGHLVPEDQFRKTKAVVEKFGAPGGVGETLQKKLLERSEQKANWVYDYWLEDMYLNNRLALPVNSSPVMVFHKQNFKGQSDVLRFAANLISGVLEYKALIDGRALPVEHARGQLAGTPLCMDQYNKVFTSYRLPGTKTDTLVAQKSTVMPEPEHIIVACKNQFFVLDVMVNFRRLNEKDLYTQLERIRKMADIEEERQPPIGLLTSDGRTQWAEARNILIKDSTNRDSLDMIERCLCLVCLDEETATELNDSNRALLMLHGGGTDKNGGNRWYDKPMQFVIGADGCCGVVCEHSPFEGIVLVQCSEYLLRYMRGSPSKLVRAASMSELPAPRRLRWKCSPDIQTFLSASADRLQKLVKNLDMNVHKFTGYGKEFIKRQKMSPDAYVQVALQFTFYRCHGRLVPTYESASIRRFQEGRVDNIRSSTPEALAFVKAMASGSKITDAEKMELLWTAIKAQTNYTILAITGMAIDNHLLGLREIAKELKLEKPELFSDTTYATSIHFTLSTSQVPTTEEMFCCYGPVVPNGYGACYNPQTDHILFCVSSFRECAETSSDLFVKTLEGCLKEMQDLCRKCNTEVKPADSTQRMEGNPKVMKNGSKS

Target ORF information:

RefSeq Version	XM_021480488.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio choline O-acetyltransferase a (chata), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480488.1

ORF Insert Sequence:

ATGCCAGTTT CAAAAAGGGA ACAATCAAAG GACACGGGAG ATCCATGTGC TCTCCCGAAG 
CTCCCCATTC CCCCCTTGAA GCAAACACTG GACATGTACC TGACATGCAT GGGTCACCTC 
GTCCCAGAGG ACCAGTTCAG AAAGACCAAG GCCGTTGTAG AGAAATTTGG AGCACCTGGT 
GGAGTGGGGG AAACCCTTCA GAAAAAGTTA CTGGAGAGAA GTGAACAAAA GGCCAATTGG 
GTGTATGATT ATTGGCTTGA AGACATGTAT TTGAACAACA GGTTAGCACT ACCTGTCAAC 
TCCAGTCCTG TTATGGTCTT TCACAAGCAG AACTTCAAGG GTCAAAGTGA TGTCCTCAGA 
TTTGCTGCTA ATCTCATTTC TGGTGTATTA GAGTATAAAG CTCTAATTGA TGGACGTGCT 
CTCCCTGTAG AGCATGCTCG TGGGCAGTTG GCCGGGACGC CCCTGTGTAT GGATCAGTAC 
AACAAGGTCT TCACGTCCTA CAGGCTGCCA GGAACAAAAA CAGACACTTT AGTGGCTCAG 
AAGAGCACAG TTATGCCTGA ACCCGAACAT ATAATAGTGG CATGTAAGAA TCAGTTTTTT 
GTTCTAGACG TGATGGTAAA CTTTCGCCGG CTGAATGAAA AAGACCTTTA CACTCAGCTG 
GAGCGAATCA GAAAGATGGC AGACATCGAA GAGGAGCGTC AGCCTCCAAT CGGCCTGCTC 
ACATCAGATG GCAGAACACA GTGGGCCGAG GCTCGCAACA TACTGATCAA AGATTCTACC 
AACAGGGACT CTCTGGACAT GATTGAGCGC TGTTTGTGTC TGGTGTGTTT GGATGAGGAA 
ACTGCCACTG AACTGAATGA CAGCAACAGA GCTTTGCTAA TGCTTCATGG AGGAGGAACG 
GACAAAAACG GGGGCAACCG ATGGTACGAC AAACCCATGC AGTTTGTAAT AGGTGCTGAT 
GGATGCTGTG GCGTCGTCTG TGAACACTCT CCTTTTGAGG GAATAGTGCT TGTGCAGTGT 
TCGGAGTATC TACTGAGATA CATGAGAGGA AGCCCTTCTA AGCTGGTGAG AGCAGCCAGC 
ATGAGTGAAC TTCCAGCCCC CAGACGACTG CGCTGGAAAT GCTCTCCAGA CATCCAAACC 
TTCCTCTCAG CCTCAGCAGA CAGATTACAG AAACTTGTAA AAAACCTGGA CATGAATGTC 
CACAAATTCA CTGGTTATGG CAAAGAGTTC ATCAAGAGAC AGAAAATGAG CCCTGATGCT 
TATGTCCAAG TTGCGCTCCA GTTTACATTT TACAGATGTC ATGGACGTCT AGTGCCCACC 
TATGAAAGTG CATCAATACG CCGCTTTCAA GAGGGACGAG TCGACAACAT TCGCTCCTCC 
ACCCCTGAAG CCTTAGCATT TGTCAAAGCT ATGGCCAGTG GCTCCAAAAT CACTGATGCT 
GAGAAAATGG AGTTGCTCTG GACTGCCATA AAAGCCCAAA CTAATTACAC AATTCTAGCA 
ATCACAGGAA TGGCAATAGA CAATCACTTG TTGGGGCTGC GAGAGATTGC CAAGGAGCTG 
AAACTGGAAA AGCCAGAGCT GTTTTCAGAT ACGACCTACG CAACCAGCAT CCACTTCACT 
CTCTCTACAA GCCAGGTTCC TACCACTGAG GAGATGTTCT GCTGCTATGG TCCAGTCGTC 
CCAAATGGCT ACGGAGCCTG CTACAATCCT CAGACGGACC ACATCCTCTT CTGCGTGTCC 
AGCTTCCGTG AATGTGCGGA GACCAGCTCA GATTTGTTTG TGAAGACTCT TGAGGGGTGC 
CTGAAGGAAA TGCAAGATCT GTGCAGAAAA TGCAACACTG AAGTTAAACC AGCTGACTCC 
ACACAAAGGA TGGAGGGAAA CCCTAAGGTA ATGAAGAATG GAAGCAAGTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleus Isthmi Is Required to Sustain Target Pursuit during Visually Guided Prey-Catching.
Current biology : CB29(11)1771-1786.e5(2019 Jun)
Henriques PM,Rahman N,Jackson SE,Bianco IH

Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene.
Gene expression patterns : GEP2812-21(2018 06)
Nikaido M,Izumi S,Ohnuki H,Takigawa Y,Yamasu K,Hatta K

Mutation of a serine near the catalytic site of the choline acetyltransferase a gene almost completely abolishes motility of the zebrafish embryo.
PloS one13(11)e0207747(2018)
Joshi S,Virdi S,Etard C,Geisler R,Strähle U

Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health.
Scientific reports7(1)16839(2017 12)
Heid J,Cencioni C,Ripa R,Baumgart M,Atlante S,Milano G,Scopece A,Kuenne C,Guenther S,Azzimato V,Farsetti A,Rossi G,Braun T,Pompilio G,Martelli F,Zeiher AM,Cellerino A,Gaetano C,Spallotta F

Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools.
Scientific reports7(1)5230(2017 07)
Förster D,Arnold-Ammer I,Laurell E,Barker AJ,Fernandes AM,Finger-Baier K,Filosa A,Helmbrecht TO,Kölsch Y,Kühn E,Robles E,Slanchev K,Thiele TR,Baier H,Kubo F

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gene@genscript.com

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chata cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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