usp47 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp47
Entrez Gene ID	100101645
Full Name	ubiquitin specific peptidase 47
Synonyms	zgc:165650
General protein information	Preferred Names ubiquitin specific peptidase 47 Names ubiquitin carboxyl-terminal hydrolase 47
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331129.1	NP_001318058.1	ubiquitin carboxyl-terminal hydrolase 47
XM_021477371.1	XP_021333046.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
XM_021477372.1	XP_021333047.1	ubiquitin carboxyl-terminal hydrolase 47 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa98348	NM_001331129.1 Latest version!	Danio rerio ubiquitin specific peptidase 47 (usp47), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa143185	XM_021477371.1 Latest version!	Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa143186	XM_021477372.1 Latest version!	Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp47 ( NM_001331129.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001318058.1 Danio rerio ubiquitin specific peptidase 47 (usp47), mRNA.

Price & Availability↑

CloneID	ODa98348
Clone ID Related Accession (Same CDS sequence)	NM_001331129.1
Accession Version	NM_001331129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504627200000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 47
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01012410.1 and CABZ01012411.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01012119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGG TGCCCAGCGA AGAAAAGCAG CTTGTGCCCA AAGAGATTGA AAGTGCAGCG 
GAGGAGCCAA GAGTGTTGTG TATAGTGCAG GACACCACCA ATGCCAAGAC CGTCAATGAG 
AGGCTGACGC TCAACCTGCC CGCCTCCACC ACTCTCAGCA AATTCTTTGA AGATGTGGCG 
CACAAGGCTG GATACGTCAA CGGCACTTTC TGCCTGTCAT GGGCCAACAC TGGCGACATG 
GCTCCTCTGG ACCAGGCCAG TGAAATGTCC CTGGTGATGT CCGGTTTTGA GGCGGGGAAA 
AGAAATTTTC TGCAGCTCAC AGACAAGGAT GGGGAGCAGC CGCAGATCGC TTCAGATGAA 
TCTGCCACAG CAGACAGCGG TGGTCTTGAT GACAGCTCTC AGGATCGCTT CATCGGACCC 
TTACCGCGGG ACGGTACTGT AGGCTGCAGC AGTGACTACA GCAGCCCCAG TTATTCCTAC 
TCCTCCATAC TCAACAAATC AGAGACAGGT TATGTTGGTT TGGTCAACCA AGCGATGACC 
TGTTACCTGA ACAGCCTCCT GCAGACCCTT TTCATGACCC CTGAGTTCAG GAATGCACTC 
TATAATTGGG AGTTTGAGGA TTCTGAGGAG GATCCGGTCA CCAGTATTCC ATACCAGCTC 
CAGAGGCTGT TTGTTCTCCT GCAGACCAGT AAAAAGCGTG CCATCGAGAC CACAGACGTG 
ACCCGCAGCT TTGGCTGGGA CAGCAGTGAG GCTTGGCAGC AGCATGACGT CCAGGAGCTG 
TGCCGAGTCA TGTTTGATGC ACTGGAGCAG AAGTGGAAAC AGACTGAGCA GGCCGACCTG 
ATCAACCAGC TTTACCAGGG CAAGCTGAAG GACTATGTGC GCTGTTTGGA GTGTGGCTAT 
GAGAGCTGGA GGATTGATAC GTACCTGGAC ATCCCTCTGG TCATCAGGCC CTTCGGCGCC 
AGCCAGGCTT TTGGCAGCGT GGAAGAAGCT CTGCAGGCCT TCATCCAGCC TGAGACTCTA 
GACGGGCCCA ATCAGTACTT CTGTGAGCGC TGCAAAAAGA AATGTGACGC CCGGAAGGGT 
CTGAGATTCC TTCATTTCCC ATACCTGCTG ACTCTGCAAC TGAAACGCTT CGACTTTGAT 
TACACCACTA TGCACCGCAT TAAACTCAAC GACCGCATGA CCTTCCCTGA GGAGCTGGAC 
ATGAGCCCCT TCATTGATGT CGAGGATGAG AAATCTCCTC AGACTGAAAG TTGCACTGAC 
AGTGGGGCAG AGAATGAAGG AAGCTGCCAC AGTGACCAGA TGAGCAACGA TTTCTCTACG 
GATGATGGTG TAGATGAGGG CATCTGTCTG GATAACGCCA GCAGCGCGGA GCGGGTGCTC 
AAACCAAAGA GCTCCTTGAC CTTTGAACTC TTCTCGGTCA TGGTGCATTC TGGGAGTGCA 
GCAGGTGGAC ACTACTATGC CTGCATCAAG TCCTTCAGTG ACAGCCAATG GTACAGCTTT 
AATGACCAGC ACGTCAGCAA GATCACGCAG GACGACATAC GGAAGACATA TGGCGGATCC 
TCAGGGAGCA GAGGCTATTA CTCCAGTGCC TTTGCCAGCT CTACAAATGC ATACATGCTG 
ATGTACAGAC TAAAAGACCC CTCCAGGAAT GCAAAGTTTC TGGAGGTGGA GGATTTTCCT 
GAGCATATAA AGAGACTGGT GCAGCGAGAG AAAGAGTCTG AGGAGCAGGA GAAGAGGCAA 
AGAGAAATTG AGCGCAACAC TTGCAAGATC AAGCTGTTCT GCATGCATCC TGTGAAGATG 
ATGATGATGG AAAATAAACT GGAGGTTCAC AAGGACAAGA CCCTCAATGA AGCAACTGAA 
ATGGCCTATA AGCTGATGGA TTTGGAGGGA GTGGTGCCCC TGGACTGCTG TCGATTGGTC 
AAATACGATG AGTTTCATGA ATATCTGGAA CGTTCGTATG AGGGTGAAGA AGACATGCCC 
ATGGGTTTGC TGCTCGGCGG GGTCAAATCT TCATACATGT TTGACCTGCT CCTGGAGATC 
CGCAGACCCG AGCAAGCGTT CCAACCCTAC AAACCTGGAG AAGTGATGGT GAAGGTCCAT 
GTGGTGGATC TAAAGAGTGA ATCTATTGCC GCACCAGTCA GTGTCAGGGC ATATCTCAAC 
CAGACTATTA CAGAGTTTAA GCAACTCATT GCACAGGCAA CGGGACTTTG TGCAGAGACG 
ATGCGTGTCG TCTTAGAGCG CTGCTATAAT GACCTTCGGC TTCTTTATGT GCCCAACAAG 
ACCCTGAAAG CAGAAGGTTT CTTCAGGAGT AACAAGGTTT TCGTGGAGAG CTCCGACTCT 
ACGGATCATC AGGCTGCCTT CACCGACTCT GTTCTGTGGA AGCTCCTGGA CCGCCACGGA 
AACACAATCA GACTCTTTGT CTCTCTGCCT GAGCAGTCGC CAGGCTCTTT CGCCAATAGA 
ACTAGTTGCC CCAAAGCCAG CGCTGACTCC GAGGACTCTT TTGATGGCGC CAAGGCCGGC 
AGAAAGTCAG TAGAGGCGAT CCTTGAAGAG AGCACAGAGA AGCTGAAGAA CCTTTCTTTA 
CAGCAACAAC AGCAGCAGCA GCAAACCTCC AGCACCAGTG ACAGTCAGAA GAGCTCCGAA 
CACAGCGATT TCGAGCACAT CGAGTCACCA GCACAGGAGC CGGATGCTTC CTCACAGCCT 
TCGCAGACGG AGAACTGCAC GCGGGCCGCT TCCGACACGG ACAACCAGTT CCCTTCTGAG 
GAGCGTTCAG ACTCGGATGT CAATAACGAC CGCAGCACCA GCTCAGTGGA CAGCGATATC 
CTGAGCTCCA GCCATAGCAG CGACACACTG TGTAATGCAG ACAGCGGCCC GCTGCCATTG 
GCTAACGGAT TGGACTCACA CAGCATCACC AGCAGCCGAC GCTCCAAGGC ACATGAGGGG 
AAGAAGGAGA CGTGGGACAC GGCAGAGGAG GACTCAGGCA CGGACAGCGA GTATGATGAG 
AACGGCAAGA GCAAAGCTGA CACGCAGTAC CTGTACTTCA AAGCAGAGCC GTACTCTCAG 
GAGGACGGGG CTGGAGACGG ACAGAAAAGT TTACTTGTTC ACGTGGACAA GAGGATCACA 
CTGGCAGCAT TCAAACAGAA TCTAGAGCCG TTTGTTGGCG TGCCCTCCAC TCAGTTCAAG 
GTCTTCCGAG TTTACGCCAA CAACCAGGAG TTTGAGAGCG TCAGGCTCAA TGAGACGCTC 
TCCTTTTTCT CCGATGACAA CAAGATCACC ATTCGATTGG GACGTGCTCT GAAGAAAGGA 
GAGTACAGGG TGAAGGTCTA CCAGCTGCTA GTCAATGACC CAGAGCCCTG TAAGTTCCTT 
TTGGACACTG TGTTCGCAAA GGGCATGACT GTTCGACAGT CTAAGGAGGA GTTACTGCCT 
CAGCTAAAAG ACCAGTGCAA ACTGGACCTC AGTATAGACA GGTTTAGACT CAGGAAGAAG 
ACCTGGAAGA ACCCAGGCAC GGTGTTTCTG GATTATCACG TCTACGAGGA GGACATCAAC 
ATCTCCAGTA ACCTGGAGGT GTTTCTGGAG GTCCTTGAAG AACCGGAGAG GATGAAGTCT 
ATGTCCCAGT TGGCGGTTCT GACCCGTCGC TGGTGTCCGG CCCAGATGAA GCTGGAGGCC 
TTTCGGGAGG TGGTGCTGGA GAGCAGCAGC GTGGAGGAGC TCAAGGAGAA GCTCAGTGAA 
GTGAGTGGAA TTCCTCTAGA AAACCTGGAG TTTGCCAAGG GACGAGGAAC TTTTCCTTGT 
GACATTTCAG TGCTGGAGAT CCATCAGGAT TTAGACTGGA ATCCAAAGGT CTCCACCCTT 
AATGTATGGC CGCTCTACAT CTGCGACGAC GGCGCTGTGA TCTTCTACAG GGACAGCAGT 
GAGGAGCTGA TGGAGCTTTC CGAAGAGGAG CGCAATGAAC TGATGAAGAA GGAGAGCAGT 
CGCCTGCTGA AGACGGGCCA CCGGGTCAGC TACTCGCCCC GCAAAGAGAA AGCACTCAAA 
ATCTACTTGG ACGGAGGGCC CACCAAAGAC CCAGGACTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318058.1
CDS	200..4264
Translation	MEMVPSEEKQLVPKEIESAAEEPRVLCIVQDTTNAKTVNERLTLNLPASTTLSKFFEDVAHKAGYVNGTFCLSWANTGDMAPLDQASEMSLVMSGFEAGKRNFLQLTDKDGEQPQIASDESATADSGGLDDSSQDRFIGPLPRDGTVGCSSDYSSPSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYNWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVRCLECGYESWRIDTYLDIPLVIRPFGASQAFGSVEEALQAFIQPETLDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSPFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDGVDEGICLDNASSAERVLKPKSSLTFELFSVMVHSGSAAGGHYYACIKSFSDSQWYSFNDQHVSKITQDDIRKTYGGSSGSRGYYSSAFASSTNAYMLMYRLKDPSRNAKFLEVEDFPEHIKRLVQREKESEEQEKRQREIERNTCKIKLFCMHPVKMMMMENKLEVHKDKTLNEATEMAYKLMDLEGVVPLDCCRLVKYDEFHEYLERSYEGEEDMPMGLLLGGVKSSYMFDLLLEIRRPEQAFQPYKPGEVMVKVHVVDLKSESIAAPVSVRAYLNQTITEFKQLIAQATGLCAETMRVVLERCYNDLRLLYVPNKTLKAEGFFRSNKVFVESSDSTDHQAAFTDSVLWKLLDRHGNTIRLFVSLPEQSPGSFANRTSCPKASADSEDSFDGAKAGRKSVEAILEESTEKLKNLSLQQQQQQQQTSSTSDSQKSSEHSDFEHIESPAQEPDASSQPSQTENCTRAASDTDNQFPSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSGPLPLANGLDSHSITSSRRSKAHEGKKETWDTAEEDSGTDSEYDENGKSKADTQYLYFKAEPYSQEDGAGDGQKSLLVHVDKRITLAAFKQNLEPFVGVPSTQFKVFRVYANNQEFESVRLNETLSFFSDDNKITIRLGRALKKGEYRVKVYQLLVNDPEPCKFLLDTVFAKGMTVRQSKEELLPQLKDQCKLDLSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNLEVFLEVLEEPERMKSMSQLAVLTRRWCPAQMKLEAFREVVLESSSVEELKEKLSEVSGIPLENLEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDSSEELMELSEEERNELMKKESSRLLKTGHRVSYSPRKEKALKIYLDGGPTKDPGLD

Target ORF information:

RefSeq Version	NM_001331129.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 47 (usp47), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331129.1

ORF Insert Sequence:

ATGGAAATGG TGCCCAGCGA AGAAAAGCAG CTTGTGCCCA AAGAGATTGA AAGTGCAGCG 
GAGGAGCCAA GAGTGTTGTG TATAGTGCAG GACACCACCA ATGCCAAGAC CGTCAATGAG 
AGGCTGACGC TCAACCTGCC CGCCTCCACC ACTCTCAGCA AATTCTTTGA AGATGTGGCG 
CACAAGGCTG GATACGTCAA CGGCACTTTC TGCCTGTCAT GGGCCAACAC TGGCGACATG 
GCTCCTCTGG ACCAGGCCAG TGAAATGTCC CTGGTGATGT CCGGTTTTGA GGCGGGGAAA 
AGAAATTTTC TGCAGCTCAC AGACAAGGAT GGGGAGCAGC CGCAGATCGC TTCAGATGAA 
TCTGCCACAG CAGACAGCGG TGGTCTTGAT GACAGCTCTC AGGATCGCTT CATCGGACCC 
TTACCGCGGG ACGGTACTGT AGGCTGCAGC AGTGACTACA GCAGCCCCAG TTATTCCTAC 
TCCTCCATAC TCAACAAATC AGAGACAGGT TATGTTGGTT TGGTCAACCA AGCGATGACC 
TGTTACCTGA ACAGCCTCCT GCAGACCCTT TTCATGACCC CTGAGTTCAG GAATGCACTC 
TATAATTGGG AGTTTGAGGA TTCTGAGGAG GATCCGGTCA CCAGTATTCC ATACCAGCTC 
CAGAGGCTGT TTGTTCTCCT GCAGACCAGT AAAAAGCGTG CCATCGAGAC CACAGACGTG 
ACCCGCAGCT TTGGCTGGGA CAGCAGTGAG GCTTGGCAGC AGCATGACGT CCAGGAGCTG 
TGCCGAGTCA TGTTTGATGC ACTGGAGCAG AAGTGGAAAC AGACTGAGCA GGCCGACCTG 
ATCAACCAGC TTTACCAGGG CAAGCTGAAG GACTATGTGC GCTGTTTGGA GTGTGGCTAT 
GAGAGCTGGA GGATTGATAC GTACCTGGAC ATCCCTCTGG TCATCAGGCC CTTCGGCGCC 
AGCCAGGCTT TTGGCAGCGT GGAAGAAGCT CTGCAGGCCT TCATCCAGCC TGAGACTCTA 
GACGGGCCCA ATCAGTACTT CTGTGAGCGC TGCAAAAAGA AATGTGACGC CCGGAAGGGT 
CTGAGATTCC TTCATTTCCC ATACCTGCTG ACTCTGCAAC TGAAACGCTT CGACTTTGAT 
TACACCACTA TGCACCGCAT TAAACTCAAC GACCGCATGA CCTTCCCTGA GGAGCTGGAC 
ATGAGCCCCT TCATTGATGT CGAGGATGAG AAATCTCCTC AGACTGAAAG TTGCACTGAC 
AGTGGGGCAG AGAATGAAGG AAGCTGCCAC AGTGACCAGA TGAGCAACGA TTTCTCTACG 
GATGATGGTG TAGATGAGGG CATCTGTCTG GATAACGCCA GCAGCGCGGA GCGGGTGCTC 
AAACCAAAGA GCTCCTTGAC CTTTGAACTC TTCTCGGTCA TGGTGCATTC TGGGAGTGCA 
GCAGGTGGAC ACTACTATGC CTGCATCAAG TCCTTCAGTG ACAGCCAATG GTACAGCTTT 
AATGACCAGC ACGTCAGCAA GATCACGCAG GACGACATAC GGAAGACATA TGGCGGATCC 
TCAGGGAGCA GAGGCTATTA CTCCAGTGCC TTTGCCAGCT CTACAAATGC ATACATGCTG 
ATGTACAGAC TAAAAGACCC CTCCAGGAAT GCAAAGTTTC TGGAGGTGGA GGATTTTCCT 
GAGCATATAA AGAGACTGGT GCAGCGAGAG AAAGAGTCTG AGGAGCAGGA GAAGAGGCAA 
AGAGAAATTG AGCGCAACAC TTGCAAGATC AAGCTGTTCT GCATGCATCC TGTGAAGATG 
ATGATGATGG AAAATAAACT GGAGGTTCAC AAGGACAAGA CCCTCAATGA AGCAACTGAA 
ATGGCCTATA AGCTGATGGA TTTGGAGGGA GTGGTGCCCC TGGACTGCTG TCGATTGGTC 
AAATACGATG AGTTTCATGA ATATCTGGAA CGTTCGTATG AGGGTGAAGA AGACATGCCC 
ATGGGTTTGC TGCTCGGCGG GGTCAAATCT TCATACATGT TTGACCTGCT CCTGGAGATC 
CGCAGACCCG AGCAAGCGTT CCAACCCTAC AAACCTGGAG AAGTGATGGT GAAGGTCCAT 
GTGGTGGATC TAAAGAGTGA ATCTATTGCC GCACCAGTCA GTGTCAGGGC ATATCTCAAC 
CAGACTATTA CAGAGTTTAA GCAACTCATT GCACAGGCAA CGGGACTTTG TGCAGAGACG 
ATGCGTGTCG TCTTAGAGCG CTGCTATAAT GACCTTCGGC TTCTTTATGT GCCCAACAAG 
ACCCTGAAAG CAGAAGGTTT CTTCAGGAGT AACAAGGTTT TCGTGGAGAG CTCCGACTCT 
ACGGATCATC AGGCTGCCTT CACCGACTCT GTTCTGTGGA AGCTCCTGGA CCGCCACGGA 
AACACAATCA GACTCTTTGT CTCTCTGCCT GAGCAGTCGC CAGGCTCTTT CGCCAATAGA 
ACTAGTTGCC CCAAAGCCAG CGCTGACTCC GAGGACTCTT TTGATGGCGC CAAGGCCGGC 
AGAAAGTCAG TAGAGGCGAT CCTTGAAGAG AGCACAGAGA AGCTGAAGAA CCTTTCTTTA 
CAGCAACAAC AGCAGCAGCA GCAAACCTCC AGCACCAGTG ACAGTCAGAA GAGCTCCGAA 
CACAGCGATT TCGAGCACAT CGAGTCACCA GCACAGGAGC CGGATGCTTC CTCACAGCCT 
TCGCAGACGG AGAACTGCAC GCGGGCCGCT TCCGACACGG ACAACCAGTT CCCTTCTGAG 
GAGCGTTCAG ACTCGGATGT CAATAACGAC CGCAGCACCA GCTCAGTGGA CAGCGATATC 
CTGAGCTCCA GCCATAGCAG CGACACACTG TGTAATGCAG ACAGCGGCCC GCTGCCATTG 
GCTAACGGAT TGGACTCACA CAGCATCACC AGCAGCCGAC GCTCCAAGGC ACATGAGGGG 
AAGAAGGAGA CGTGGGACAC GGCAGAGGAG GACTCAGGCA CGGACAGCGA GTATGATGAG 
AACGGCAAGA GCAAAGCTGA CACGCAGTAC CTGTACTTCA AAGCAGAGCC GTACTCTCAG 
GAGGACGGGG CTGGAGACGG ACAGAAAAGT TTACTTGTTC ACGTGGACAA GAGGATCACA 
CTGGCAGCAT TCAAACAGAA TCTAGAGCCG TTTGTTGGCG TGCCCTCCAC TCAGTTCAAG 
GTCTTCCGAG TTTACGCCAA CAACCAGGAG TTTGAGAGCG TCAGGCTCAA TGAGACGCTC 
TCCTTTTTCT CCGATGACAA CAAGATCACC ATTCGATTGG GACGTGCTCT GAAGAAAGGA 
GAGTACAGGG TGAAGGTCTA CCAGCTGCTA GTCAATGACC CAGAGCCCTG TAAGTTCCTT 
TTGGACACTG TGTTCGCAAA GGGCATGACT GTTCGACAGT CTAAGGAGGA GTTACTGCCT 
CAGCTAAAAG ACCAGTGCAA ACTGGACCTC AGTATAGACA GGTTTAGACT CAGGAAGAAG 
ACCTGGAAGA ACCCAGGCAC GGTGTTTCTG GATTATCACG TCTACGAGGA GGACATCAAC 
ATCTCCAGTA ACCTGGAGGT GTTTCTGGAG GTCCTTGAAG AACCGGAGAG GATGAAGTCT 
ATGTCCCAGT TGGCGGTTCT GACCCGTCGC TGGTGTCCGG CCCAGATGAA GCTGGAGGCC 
TTTCGGGAGG TGGTGCTGGA GAGCAGCAGC GTGGAGGAGC TCAAGGAGAA GCTCAGTGAA 
GTGAGTGGAA TTCCTCTAGA AAACCTGGAG TTTGCCAAGG GACGAGGAAC TTTTCCTTGT 
GACATTTCAG TGCTGGAGAT CCATCAGGAT TTAGACTGGA ATCCAAAGGT CTCCACCCTT 
AATGTATGGC CGCTCTACAT CTGCGACGAC GGCGCTGTGA TCTTCTACAG GGACAGCAGT 
GAGGAGCTGA TGGAGCTTTC CGAAGAGGAG CGCAATGAAC TGATGAAGAA GGAGAGCAGT 
CGCCTGCTGA AGACGGGCCA CCGGGTCAGC TACTCGCCCC GCAAAGAGAA AGCACTCAAA 
ATCTACTTGG ACGGAGGGCC CACCAAAGAC CCAGGACTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp47 ( XM_021477371.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333046.1 Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143185
Clone ID Related Accession (Same CDS sequence)	XM_021477371.1
Accession Version	XM_021477371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGG TGCCCAGCGA AGAAAAGCAG CTTGTGCCCA AAGAGGGCAT TTTTTGGAGT 
TGCAGGCAGA GTATTTTTGA GGAAATGCAG AAGAAGTTCT CGCAGATTGA AAGTGCAGCG 
GAGGAGCCAA GAGTGTTGTG TATAGTGCAG GACACCACCA ATGCCAAGAC CGTCAATGAG 
AGGCTGACGC TCAACCTGCC CGCCTCCACC ACTCTCAGCA AATTCTTTGA AGATGTGGCG 
CACAAGGCTG GATACGTCAA CGGCACTTTC TGCCTGTCAT GGGCCAACAC TGGCGACATG 
GCTCCTCTGG ACCAGGCCAG TGAAATGTCC CTGGTGATGT CCGGTTTTGA GGCGGGGAAA 
AGAAATTTTC TGCAGCTCAC AGACAAGGAT GGGGAGCAGC CGCAGATCGC TTCAGATGAA 
TCTGCCACAG CAGACAGCGG TGGTCTTGAT GACAGCTCTC AGGATCGCTT CATCGGACCC 
TTACCGCGGG ACGGTACTGT AGGCTGCAGC AGTGACTACA GCAGCCCCAG TTATTCCTAC 
TCCTCCATAC TCAACAAATC AGAGACAGGT TATGTTGGTT TGGTCAACCA AGCGATGACC 
TGTTACCTGA ACAGCCTCCT GCAGACCCTT TTCATGACCC CTGAGTTCAG GAATGCACTC 
TATAATTGGG AGTTTGAGGA TTCTGAGGAG GATCCGGTCA CCAGTATTCC ATACCAGCTC 
CAGAGGCTGT TTGTTCTCCT GCAGACCAGT AAAAAGCGTG CCATCGAGAC CACAGACGTG 
ACCCGCAGCT TTGGCTGGGA CAGCAGTGAG GCTTGGCAGC AGCATGACGT CCAGGAGCTG 
TGCCGAGTCA TGTTTGATGC ACTGGAGCAG AAGTGGAAAC AGACTGAGCA GGCCGACCTG 
ATCAACCAGC TTTACCAGGG CAAGCTGAAG GACTATGTGC GCTGTTTGGA GTGTGGCTAT 
GAGAGCTGGA GGATTGATAC GTACCTGGAC ATCCCTCTGG TCATCAGGCC CTTCGGCGCC 
AGCCAGGCTT TTGGCAGCGT GGAAGAAGCT CTGCAGGCCT TCATCCAGCC TGAGACTCTA 
GACGGGCCCA ATCAGTACTT CTGTGAGCGC TGCAAAAAGA AATGTGACGC CCGGAAGGGT 
CTGAGATTCC TTCATTTCCC ATACCTGCTG ACTCTGCAAC TGAAACGCTT CGACTTTGAT 
TACACCACTA TGCACCGCAT TAAACTCAAC GACCGCATGA CCTTCCCTGA GGAGCTGGAC 
ATGAGCCCCT TCATTGATGT CGAGGATGAG AAATCTCCTC AGACTGAAAG TTGCACTGAC 
AGTGGGGCAG AGAATGAAGG AAGCTGCCAC AGTGACCAGA TGAGCAACGA TTTCTCTACG 
GATGATGGTG TAGATGAGGG CATCTGTCTG GATAACGCCA GCAGCGCGGA GCGGGTGCTC 
AAACCAAAGA GCTCCTTGAC CTTTGAACTC TTCTCGGTCA TGGTGCATTC TGGGAGTGCA 
GCAGGTGGAC ACTACTATGC CTGCATCAAG TCCTTCAGTG ACAGCCAATG GTACAGCTTT 
AATGACCAGC ACGTCAGCAA GATCACGCAG GACGACATAC GGAAGACATA TGGCGGATCC 
TCAGGGAGCA GAGGCTATTA CTCCAGTGCC TTTGCCAGCT CTACAAATGC ATACATGCTG 
ATGTACAGAC TAAAAGACCC CTCCAGGAAT GCAAAGTTTC TGGAGGTGGA GGATTTTCCT 
GAGCATATAA AGAGACTGGT GCAGCGAGAG AAAGAGTCTG AGGAGCAGGA GAAGAGGCAA 
AGAGAAATTG AGCGCAACAC TTGCAAGATC AAGCTGTTCT GCATGCATCC TGTGAAGATG 
ATGATGATGG AAAATAAACT GGAGGTTCAC AAGGACAAGA CCCTCAATGA AGCAACTGAA 
ATGGCCTATA AGCTGATGGA TTTGGAGGGA GTGGTGCCCC TGGACTGCTG TCGATTGGTC 
AAATACGATG AGTTTCATGA ATATCTGGAA CGTTCGTATG AGGGTGAAGA AGACATGCCC 
ATGGGTTTGC TGCTCGGCGG GGTCAAATCT TCATACATGT TTGACCTGCT CCTGGAGATC 
CGCAGACCCG AGCAAGCGTT CCAACCCTAC AAACCTGGAG AAGTGATGGT GAAGGTCCAT 
GTGGTGGATC TAAAGAGTGA ATCTATTGCC GCACCAGTCA GTGTCAGGGC ATATCTCAAC 
CAGACTATTA CAGAGTTTAA GCAACTCATT GCACAGGCAA CGGGACTTTG TGCAGAGACG 
ATGCGTGTCG TCTTAGAGCG CTGCTATAAT GACCTTCGGC TTCTTTATGT GCCCAACAAG 
ACCCTGAAAG CAGAAGGTTT CTTCAGGAGT AACAAGGTTT TCGTGGAGAG CTCCGACTCT 
ACGGATCATC AGGCTGCCTT CACCGACTCT GTTCTGTGGA AGCTCCTGGA CCGCCACGGA 
AACACAATCA GACTCTTTGT CTCTCTGCCT GAGCAGTCGC CAGGCTCTTT CGCCAATAGA 
ACTAGTTGCC CCAAAGCCAG CGCTGACTCC GAGGACTCTT TTGATGGCGC CAAGGCCGGC 
AGAAAGTCAG TAGAGGCGAT CCTTGAAGAG AGCACAGAGA AGCTGAAGAA CCTTTCTTTA 
CAGCAACAAC AGCAGCAGCA GCAAACCTCC AGCACCAGTG ACAGTCAGAA GAGCTCCGAA 
CACAGCGATT TCGAGCACAT CGAGTCACCA GCACAGGAGC CGGATGCTTC CTCACAGCCT 
TCGCAGACGG AGAACTGCAC GCGGGCCGCT TCCGACACGG ACAACCAGTT CCCTTCTGAG 
GAGCGTTCAG ACTCGGATGT CAATAACGAC CGCAGCACCA GCTCAGTGGA CAGCGATATC 
CTGAGCTCCA GCCATAGCAG CGACACACTG TGTAATGCAG ACAGCGGCCC GCTGCCATTG 
GCTAACGGAT TGGACTCACA CAGCATCACC AGCAGCCGAC GCTCCAAGGC ACATGAGGGG 
AAGAAGGAGA CGTGGGACAC GGCAGAGGAG GACTCAGGCA CGGACAGCGA GTATGATGAG 
AACGGCAAGA GCAAAGCTGA CACGCAGTAC CTGTACTTCA AAGCAGAGCC GTACTCTCAG 
GAGGACGGGG CTGGAGACGG ACAGAAAAGT TTACTTGTTC ACGTGGACAA GAGGATCACA 
CTGGCAGCAT TCAAACAGAA TCTAGAGCCG TTTGTTGGCG TGCCCTCCAC TCAGTTCAAG 
GTCTTCCGAG TTTACGCCAA CAACCAGGAG TTTGAGAGCG TCAGGCTCAA TGAGACGCTC 
TCCTTTTTCT CCGATGACAA CAAGATCACC ATTCGATTGG GACGTGCTCT GAAGAAAGGA 
GAGTACAGGG TGAAGGTCTA CCAGCTGCTA GTCAATGACC CAGAGCCCTG TAAGTTCCTT 
TTGGACACTG TGTTCGCAAA GGGCATGACT GTTCGACAGT CTAAGGAGGA GTTACTGCCT 
CAGCTAAAAG ACCAGTGCAA ACTGGACCTC AGTATAGACA GGTTTAGACT CAGGAAGAAG 
ACCTGGAAGA ACCCAGGCAC GGTGTTTCTG GATTATCACG TCTACGAGGA GGACATCAAC 
ATCTCCAGTA ACCTGGAGGT GTTTCTGGAG GTCCTTGAAG AACCGGAGAG GATGAAGTCT 
ATGTCCCAGT TGGCGGTTCT GACCCGTCGC TGGTGTCCGG CCCAGATGAA GCTGGAGGCC 
TTTCGGGAGG TGGTGCTGGA GAGCAGCAGC GTGGAGGAGC TCAAGGAGAA GCTCAGTGAA 
GTGAGTGGAA TTCCTCTAGA AAACCTGGAG TTTGCCAAGG GACGAGGAAC TTTTCCTTGT 
GACATTTCAG TGCTGGAGAT CCATCAGGAT TTAGACTGGA ATCCAAAGGT CTCCACCCTT 
AATGTATGGC CGCTCTACAT CTGCGACGAC GGCGCTGTGA TCTTCTACAG GGACAGCAGT 
GAGGAGCTGA TGGAGCTTTC CGAAGAGGAG CGCAATGAAC TGATGAAGAA GGAGAGCAGT 
CGCCTGCTGA AGACGGGCCA CCGGGTCAGC TACTCGCCCC GCAAAGAGAA AGCACTCAAA 
ATCTACTTGG ACGGAGGGCC CACCAAAGAC CCAGGACTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333046.1
CDS	336..4460
Translation	MEMVPSEEKQLVPKEGIFWSCRQSIFEEMQKKFSQIESAAEEPRVLCIVQDTTNAKTVNERLTLNLPASTTLSKFFEDVAHKAGYVNGTFCLSWANTGDMAPLDQASEMSLVMSGFEAGKRNFLQLTDKDGEQPQIASDESATADSGGLDDSSQDRFIGPLPRDGTVGCSSDYSSPSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYNWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVRCLECGYESWRIDTYLDIPLVIRPFGASQAFGSVEEALQAFIQPETLDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSPFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDGVDEGICLDNASSAERVLKPKSSLTFELFSVMVHSGSAAGGHYYACIKSFSDSQWYSFNDQHVSKITQDDIRKTYGGSSGSRGYYSSAFASSTNAYMLMYRLKDPSRNAKFLEVEDFPEHIKRLVQREKESEEQEKRQREIERNTCKIKLFCMHPVKMMMMENKLEVHKDKTLNEATEMAYKLMDLEGVVPLDCCRLVKYDEFHEYLERSYEGEEDMPMGLLLGGVKSSYMFDLLLEIRRPEQAFQPYKPGEVMVKVHVVDLKSESIAAPVSVRAYLNQTITEFKQLIAQATGLCAETMRVVLERCYNDLRLLYVPNKTLKAEGFFRSNKVFVESSDSTDHQAAFTDSVLWKLLDRHGNTIRLFVSLPEQSPGSFANRTSCPKASADSEDSFDGAKAGRKSVEAILEESTEKLKNLSLQQQQQQQQTSSTSDSQKSSEHSDFEHIESPAQEPDASSQPSQTENCTRAASDTDNQFPSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSGPLPLANGLDSHSITSSRRSKAHEGKKETWDTAEEDSGTDSEYDENGKSKADTQYLYFKAEPYSQEDGAGDGQKSLLVHVDKRITLAAFKQNLEPFVGVPSTQFKVFRVYANNQEFESVRLNETLSFFSDDNKITIRLGRALKKGEYRVKVYQLLVNDPEPCKFLLDTVFAKGMTVRQSKEELLPQLKDQCKLDLSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNLEVFLEVLEEPERMKSMSQLAVLTRRWCPAQMKLEAFREVVLESSSVEELKEKLSEVSGIPLENLEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDSSEELMELSEEERNELMKKESSRLLKTGHRVSYSPRKEKALKIYLDGGPTKDPGLD

Target ORF information:

RefSeq Version	XM_021477371.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477371.1

ORF Insert Sequence:

ATGGAAATGG TGCCCAGCGA AGAAAAGCAG CTTGTGCCCA AAGAGGGCAT TTTTTGGAGT 
TGCAGGCAGA GTATTTTTGA GGAAATGCAG AAGAAGTTCT CGCAGATTGA AAGTGCAGCG 
GAGGAGCCAA GAGTGTTGTG TATAGTGCAG GACACCACCA ATGCCAAGAC CGTCAATGAG 
AGGCTGACGC TCAACCTGCC CGCCTCCACC ACTCTCAGCA AATTCTTTGA AGATGTGGCG 
CACAAGGCTG GATACGTCAA CGGCACTTTC TGCCTGTCAT GGGCCAACAC TGGCGACATG 
GCTCCTCTGG ACCAGGCCAG TGAAATGTCC CTGGTGATGT CCGGTTTTGA GGCGGGGAAA 
AGAAATTTTC TGCAGCTCAC AGACAAGGAT GGGGAGCAGC CGCAGATCGC TTCAGATGAA 
TCTGCCACAG CAGACAGCGG TGGTCTTGAT GACAGCTCTC AGGATCGCTT CATCGGACCC 
TTACCGCGGG ACGGTACTGT AGGCTGCAGC AGTGACTACA GCAGCCCCAG TTATTCCTAC 
TCCTCCATAC TCAACAAATC AGAGACAGGT TATGTTGGTT TGGTCAACCA AGCGATGACC 
TGTTACCTGA ACAGCCTCCT GCAGACCCTT TTCATGACCC CTGAGTTCAG GAATGCACTC 
TATAATTGGG AGTTTGAGGA TTCTGAGGAG GATCCGGTCA CCAGTATTCC ATACCAGCTC 
CAGAGGCTGT TTGTTCTCCT GCAGACCAGT AAAAAGCGTG CCATCGAGAC CACAGACGTG 
ACCCGCAGCT TTGGCTGGGA CAGCAGTGAG GCTTGGCAGC AGCATGACGT CCAGGAGCTG 
TGCCGAGTCA TGTTTGATGC ACTGGAGCAG AAGTGGAAAC AGACTGAGCA GGCCGACCTG 
ATCAACCAGC TTTACCAGGG CAAGCTGAAG GACTATGTGC GCTGTTTGGA GTGTGGCTAT 
GAGAGCTGGA GGATTGATAC GTACCTGGAC ATCCCTCTGG TCATCAGGCC CTTCGGCGCC 
AGCCAGGCTT TTGGCAGCGT GGAAGAAGCT CTGCAGGCCT TCATCCAGCC TGAGACTCTA 
GACGGGCCCA ATCAGTACTT CTGTGAGCGC TGCAAAAAGA AATGTGACGC CCGGAAGGGT 
CTGAGATTCC TTCATTTCCC ATACCTGCTG ACTCTGCAAC TGAAACGCTT CGACTTTGAT 
TACACCACTA TGCACCGCAT TAAACTCAAC GACCGCATGA CCTTCCCTGA GGAGCTGGAC 
ATGAGCCCCT TCATTGATGT CGAGGATGAG AAATCTCCTC AGACTGAAAG TTGCACTGAC 
AGTGGGGCAG AGAATGAAGG AAGCTGCCAC AGTGACCAGA TGAGCAACGA TTTCTCTACG 
GATGATGGTG TAGATGAGGG CATCTGTCTG GATAACGCCA GCAGCGCGGA GCGGGTGCTC 
AAACCAAAGA GCTCCTTGAC CTTTGAACTC TTCTCGGTCA TGGTGCATTC TGGGAGTGCA 
GCAGGTGGAC ACTACTATGC CTGCATCAAG TCCTTCAGTG ACAGCCAATG GTACAGCTTT 
AATGACCAGC ACGTCAGCAA GATCACGCAG GACGACATAC GGAAGACATA TGGCGGATCC 
TCAGGGAGCA GAGGCTATTA CTCCAGTGCC TTTGCCAGCT CTACAAATGC ATACATGCTG 
ATGTACAGAC TAAAAGACCC CTCCAGGAAT GCAAAGTTTC TGGAGGTGGA GGATTTTCCT 
GAGCATATAA AGAGACTGGT GCAGCGAGAG AAAGAGTCTG AGGAGCAGGA GAAGAGGCAA 
AGAGAAATTG AGCGCAACAC TTGCAAGATC AAGCTGTTCT GCATGCATCC TGTGAAGATG 
ATGATGATGG AAAATAAACT GGAGGTTCAC AAGGACAAGA CCCTCAATGA AGCAACTGAA 
ATGGCCTATA AGCTGATGGA TTTGGAGGGA GTGGTGCCCC TGGACTGCTG TCGATTGGTC 
AAATACGATG AGTTTCATGA ATATCTGGAA CGTTCGTATG AGGGTGAAGA AGACATGCCC 
ATGGGTTTGC TGCTCGGCGG GGTCAAATCT TCATACATGT TTGACCTGCT CCTGGAGATC 
CGCAGACCCG AGCAAGCGTT CCAACCCTAC AAACCTGGAG AAGTGATGGT GAAGGTCCAT 
GTGGTGGATC TAAAGAGTGA ATCTATTGCC GCACCAGTCA GTGTCAGGGC ATATCTCAAC 
CAGACTATTA CAGAGTTTAA GCAACTCATT GCACAGGCAA CGGGACTTTG TGCAGAGACG 
ATGCGTGTCG TCTTAGAGCG CTGCTATAAT GACCTTCGGC TTCTTTATGT GCCCAACAAG 
ACCCTGAAAG CAGAAGGTTT CTTCAGGAGT AACAAGGTTT TCGTGGAGAG CTCCGACTCT 
ACGGATCATC AGGCTGCCTT CACCGACTCT GTTCTGTGGA AGCTCCTGGA CCGCCACGGA 
AACACAATCA GACTCTTTGT CTCTCTGCCT GAGCAGTCGC CAGGCTCTTT CGCCAATAGA 
ACTAGTTGCC CCAAAGCCAG CGCTGACTCC GAGGACTCTT TTGATGGCGC CAAGGCCGGC 
AGAAAGTCAG TAGAGGCGAT CCTTGAAGAG AGCACAGAGA AGCTGAAGAA CCTTTCTTTA 
CAGCAACAAC AGCAGCAGCA GCAAACCTCC AGCACCAGTG ACAGTCAGAA GAGCTCCGAA 
CACAGCGATT TCGAGCACAT CGAGTCACCA GCACAGGAGC CGGATGCTTC CTCACAGCCT 
TCGCAGACGG AGAACTGCAC GCGGGCCGCT TCCGACACGG ACAACCAGTT CCCTTCTGAG 
GAGCGTTCAG ACTCGGATGT CAATAACGAC CGCAGCACCA GCTCAGTGGA CAGCGATATC 
CTGAGCTCCA GCCATAGCAG CGACACACTG TGTAATGCAG ACAGCGGCCC GCTGCCATTG 
GCTAACGGAT TGGACTCACA CAGCATCACC AGCAGCCGAC GCTCCAAGGC ACATGAGGGG 
AAGAAGGAGA CGTGGGACAC GGCAGAGGAG GACTCAGGCA CGGACAGCGA GTATGATGAG 
AACGGCAAGA GCAAAGCTGA CACGCAGTAC CTGTACTTCA AAGCAGAGCC GTACTCTCAG 
GAGGACGGGG CTGGAGACGG ACAGAAAAGT TTACTTGTTC ACGTGGACAA GAGGATCACA 
CTGGCAGCAT TCAAACAGAA TCTAGAGCCG TTTGTTGGCG TGCCCTCCAC TCAGTTCAAG 
GTCTTCCGAG TTTACGCCAA CAACCAGGAG TTTGAGAGCG TCAGGCTCAA TGAGACGCTC 
TCCTTTTTCT CCGATGACAA CAAGATCACC ATTCGATTGG GACGTGCTCT GAAGAAAGGA 
GAGTACAGGG TGAAGGTCTA CCAGCTGCTA GTCAATGACC CAGAGCCCTG TAAGTTCCTT 
TTGGACACTG TGTTCGCAAA GGGCATGACT GTTCGACAGT CTAAGGAGGA GTTACTGCCT 
CAGCTAAAAG ACCAGTGCAA ACTGGACCTC AGTATAGACA GGTTTAGACT CAGGAAGAAG 
ACCTGGAAGA ACCCAGGCAC GGTGTTTCTG GATTATCACG TCTACGAGGA GGACATCAAC 
ATCTCCAGTA ACCTGGAGGT GTTTCTGGAG GTCCTTGAAG AACCGGAGAG GATGAAGTCT 
ATGTCCCAGT TGGCGGTTCT GACCCGTCGC TGGTGTCCGG CCCAGATGAA GCTGGAGGCC 
TTTCGGGAGG TGGTGCTGGA GAGCAGCAGC GTGGAGGAGC TCAAGGAGAA GCTCAGTGAA 
GTGAGTGGAA TTCCTCTAGA AAACCTGGAG TTTGCCAAGG GACGAGGAAC TTTTCCTTGT 
GACATTTCAG TGCTGGAGAT CCATCAGGAT TTAGACTGGA ATCCAAAGGT CTCCACCCTT 
AATGTATGGC CGCTCTACAT CTGCGACGAC GGCGCTGTGA TCTTCTACAG GGACAGCAGT 
GAGGAGCTGA TGGAGCTTTC CGAAGAGGAG CGCAATGAAC TGATGAAGAA GGAGAGCAGT 
CGCCTGCTGA AGACGGGCCA CCGGGTCAGC TACTCGCCCC GCAAAGAGAA AGCACTCAAA 
ATCTACTTGG ACGGAGGGCC CACCAAAGAC CCAGGACTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp47 ( XM_021477372.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333047.1 Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X2, mRNA.

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CloneID	ODa143186
Clone ID Related Accession (Same CDS sequence)	XM_021477372.1
Accession Version	XM_021477372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 47 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTC TGGACCAGGC CAGTGAAATG TCCCTGGTGA TGTCCGGTTT TGAGGCGGGG 
AAAAGAAATT TTCTGCAGCT CACAGACAAG GATGGGGAGC AGCCGCAGAT CGCTTCAGAT 
GAATCTGCCA CAGCAGACAG CGGTGGTCTT GATGACAGCT CTCAGGATCG CTTCATCGGA 
CCCTTACCGC GGGACGGTAC TGTAGGCTGC AGCAGTGACT ACAGCAGCCC CAGTTATTCC 
TACTCCTCCA TACTCAACAA ATCAGAGACA GGTTATGTTG GTTTGGTCAA CCAAGCGATG 
ACCTGTTACC TGAACAGCCT CCTGCAGACC CTTTTCATGA CCCCTGAGTT CAGGAATGCA 
CTCTATAATT GGGAGTTTGA GGATTCTGAG GAGGATCCGG TCACCAGTAT TCCATACCAG 
CTCCAGAGGC TGTTTGTTCT CCTGCAGACC AGTAAAAAGC GTGCCATCGA GACCACAGAC 
GTGACCCGCA GCTTTGGCTG GGACAGCAGT GAGGCTTGGC AGCAGCATGA CGTCCAGGAG 
CTGTGCCGAG TCATGTTTGA TGCACTGGAG CAGAAGTGGA AACAGACTGA GCAGGCCGAC 
CTGATCAACC AGCTTTACCA GGGCAAGCTG AAGGACTATG TGCGCTGTTT GGAGTGTGGC 
TATGAGAGCT GGAGGATTGA TACGTACCTG GACATCCCTC TGGTCATCAG GCCCTTCGGC 
GCCAGCCAGG CTTTTGGCAG CGTGGAAGAA GCTCTGCAGG CCTTCATCCA GCCTGAGACT 
CTAGACGGGC CCAATCAGTA CTTCTGTGAG CGCTGCAAAA AGAAATGTGA CGCCCGGAAG 
GGTCTGAGAT TCCTTCATTT CCCATACCTG CTGACTCTGC AACTGAAACG CTTCGACTTT 
GATTACACCA CTATGCACCG CATTAAACTC AACGACCGCA TGACCTTCCC TGAGGAGCTG 
GACATGAGCC CCTTCATTGA TGTCGAGGAT GAGAAATCTC CTCAGACTGA AAGTTGCACT 
GACAGTGGGG CAGAGAATGA AGGAAGCTGC CACAGTGACC AGATGAGCAA CGATTTCTCT 
ACGGATGATG GTGTAGATGA GGGCATCTGT CTGGATAACG CCAGCAGCGC GGAGCGGGTG 
CTCAAACCAA AGAGCTCCTT GACCTTTGAA CTCTTCTCGG TCATGGTGCA TTCTGGGAGT 
GCAGCAGGTG GACACTACTA TGCCTGCATC AAGTCCTTCA GTGACAGCCA ATGGTACAGC 
TTTAATGACC AGCACGTCAG CAAGATCACG CAGGACGACA TACGGAAGAC ATATGGCGGA 
TCCTCAGGGA GCAGAGGCTA TTACTCCAGT GCCTTTGCCA GCTCTACAAA TGCATACATG 
CTGATGTACA GACTAAAAGA CCCCTCCAGG AATGCAAAGT TTCTGGAGGT GGAGGATTTT 
CCTGAGCATA TAAAGAGACT GGTGCAGCGA GAGAAAGAGT CTGAGGAGCA GGAGAAGAGG 
CAAAGAGAAA TTGAGCGCAA CACTTGCAAG ATCAAGCTGT TCTGCATGCA TCCTGTGAAG 
ATGATGATGA TGGAAAATAA ACTGGAGGTT CACAAGGACA AGACCCTCAA TGAAGCAACT 
GAAATGGCCT ATAAGCTGAT GGATTTGGAG GGAGTGGTGC CCCTGGACTG CTGTCGATTG 
GTCAAATACG ATGAGTTTCA TGAATATCTG GAACGTTCGT ATGAGGGTGA AGAAGACATG 
CCCATGGGTT TGCTGCTCGG CGGGGTCAAA TCTTCATACA TGTTTGACCT GCTCCTGGAG 
ATCCGCAGAC CCGAGCAAGC GTTCCAACCC TACAAACCTG GAGAAGTGAT GGTGAAGGTC 
CATGTGGTGG ATCTAAAGAG TGAATCTATT GCCGCACCAG TCAGTGTCAG GGCATATCTC 
AACCAGACTA TTACAGAGTT TAAGCAACTC ATTGCACAGG CAACGGGACT TTGTGCAGAG 
ACGATGCGTG TCGTCTTAGA GCGCTGCTAT AATGACCTTC GGCTTCTTTA TGTGCCCAAC 
AAGACCCTGA AAGCAGAAGG TTTCTTCAGG AGTAACAAGG TTTTCGTGGA GAGCTCCGAC 
TCTACGGATC ATCAGGCTGC CTTCACCGAC TCTGTTCTGT GGAAGCTCCT GGACCGCCAC 
GGAAACACAA TCAGACTCTT TGTCTCTCTG CCTGAGCAGT CGCCAGGCTC TTTCGCCAAT 
AGAACTAGTT GCCCCAAAGC CAGCGCTGAC TCCGAGGACT CTTTTGATGG CGCCAAGGCC 
GGCAGAAAGT CAGTAGAGGC GATCCTTGAA GAGAGCACAG AGAAGCTGAA GAACCTTTCT 
TTACAGCAAC AACAGCAGCA GCAGCAAACC TCCAGCACCA GTGACAGTCA GAAGAGCTCC 
GAACACAGCG ATTTCGAGCA CATCGAGTCA CCAGCACAGG AGCCGGATGC TTCCTCACAG 
CCTTCGCAGA CGGAGAACTG CACGCGGGCC GCTTCCGACA CGGACAACCA GTTCCCTTCT 
GAGGAGCGTT CAGACTCGGA TGTCAATAAC GACCGCAGCA CCAGCTCAGT GGACAGCGAT 
ATCCTGAGCT CCAGCCATAG CAGCGACACA CTGTGTAATG CAGACAGCGG CCCGCTGCCA 
TTGGCTAACG GATTGGACTC ACACAGCATC ACCAGCAGCC GACGCTCCAA GGCACATGAG 
GGGAAGAAGG AGACGTGGGA CACGGCAGAG GAGGACTCAG GCACGGACAG CGAGTATGAT 
GAGAACGGCA AGAGCAAAGC TGACACGCAG TACCTGTACT TCAAAGCAGA GCCGTACTCT 
CAGGAGGACG GGGCTGGAGA CGGACAGAAA AGTTTACTTG TTCACGTGGA CAAGAGGATC 
ACACTGGCAG CATTCAAACA GAATCTAGAG CCGTTTGTTG GCGTGCCCTC CACTCAGTTC 
AAGGTCTTCC GAGTTTACGC CAACAACCAG GAGTTTGAGA GCGTCAGGCT CAATGAGACG 
CTCTCCTTTT TCTCCGATGA CAACAAGATC ACCATTCGAT TGGGACGTGC TCTGAAGAAA 
GGAGAGTACA GGGTGAAGGT CTACCAGCTG CTAGTCAATG ACCCAGAGCC CTGTAAGTTC 
CTTTTGGACA CTGTGTTCGC AAAGGGCATG ACTGTTCGAC AGTCTAAGGA GGAGTTACTG 
CCTCAGCTAA AAGACCAGTG CAAACTGGAC CTCAGTATAG ACAGGTTTAG ACTCAGGAAG 
AAGACCTGGA AGAACCCAGG CACGGTGTTT CTGGATTATC ACGTCTACGA GGAGGACATC 
AACATCTCCA GTAACCTGGA GGTGTTTCTG GAGGTCCTTG AAGAACCGGA GAGGATGAAG 
TCTATGTCCC AGTTGGCGGT TCTGACCCGT CGCTGGTGTC CGGCCCAGAT GAAGCTGGAG 
GCCTTTCGGG AGGTGGTGCT GGAGAGCAGC AGCGTGGAGG AGCTCAAGGA GAAGCTCAGT 
GAAGTGAGTG GAATTCCTCT AGAAAACCTG GAGTTTGCCA AGGGACGAGG AACTTTTCCT 
TGTGACATTT CAGTGCTGGA GATCCATCAG GATTTAGACT GGAATCCAAA GGTCTCCACC 
CTTAATGTAT GGCCGCTCTA CATCTGCGAC GACGGCGCTG TGATCTTCTA CAGGGACAGC 
AGTGAGGAGC TGATGGAGCT TTCCGAAGAG GAGCGCAATG AACTGATGAA GAAGGAGAGC 
AGTCGCCTGC TGAAGACGGG CCACCGGGTC AGCTACTCGC CCCGCAAAGA GAAAGCACTC 
AAAATCTACT TGGACGGAGG GCCCACCAAA GACCCAGGAC TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333047.1
CDS	17..3844
Translation	MAPLDQASEMSLVMSGFEAGKRNFLQLTDKDGEQPQIASDESATADSGGLDDSSQDRFIGPLPRDGTVGCSSDYSSPSYSYSSILNKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYNWEFEDSEEDPVTSIPYQLQRLFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINQLYQGKLKDYVRCLECGYESWRIDTYLDIPLVIRPFGASQAFGSVEEALQAFIQPETLDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMSPFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSTDDGVDEGICLDNASSAERVLKPKSSLTFELFSVMVHSGSAAGGHYYACIKSFSDSQWYSFNDQHVSKITQDDIRKTYGGSSGSRGYYSSAFASSTNAYMLMYRLKDPSRNAKFLEVEDFPEHIKRLVQREKESEEQEKRQREIERNTCKIKLFCMHPVKMMMMENKLEVHKDKTLNEATEMAYKLMDLEGVVPLDCCRLVKYDEFHEYLERSYEGEEDMPMGLLLGGVKSSYMFDLLLEIRRPEQAFQPYKPGEVMVKVHVVDLKSESIAAPVSVRAYLNQTITEFKQLIAQATGLCAETMRVVLERCYNDLRLLYVPNKTLKAEGFFRSNKVFVESSDSTDHQAAFTDSVLWKLLDRHGNTIRLFVSLPEQSPGSFANRTSCPKASADSEDSFDGAKAGRKSVEAILEESTEKLKNLSLQQQQQQQQTSSTSDSQKSSEHSDFEHIESPAQEPDASSQPSQTENCTRAASDTDNQFPSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADSGPLPLANGLDSHSITSSRRSKAHEGKKETWDTAEEDSGTDSEYDENGKSKADTQYLYFKAEPYSQEDGAGDGQKSLLVHVDKRITLAAFKQNLEPFVGVPSTQFKVFRVYANNQEFESVRLNETLSFFSDDNKITIRLGRALKKGEYRVKVYQLLVNDPEPCKFLLDTVFAKGMTVRQSKEELLPQLKDQCKLDLSIDRFRLRKKTWKNPGTVFLDYHVYEEDINISSNLEVFLEVLEEPERMKSMSQLAVLTRRWCPAQMKLEAFREVVLESSSVEELKEKLSEVSGIPLENLEFAKGRGTFPCDISVLEIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDSSEELMELSEEERNELMKKESSRLLKTGHRVSYSPRKEKALKIYLDGGPTKDPGLD

Target ORF information:

RefSeq Version	XM_021477372.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 47 (usp47), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477372.1

ORF Insert Sequence:

ATGGCTCCTC TGGACCAGGC CAGTGAAATG TCCCTGGTGA TGTCCGGTTT TGAGGCGGGG 
AAAAGAAATT TTCTGCAGCT CACAGACAAG GATGGGGAGC AGCCGCAGAT CGCTTCAGAT 
GAATCTGCCA CAGCAGACAG CGGTGGTCTT GATGACAGCT CTCAGGATCG CTTCATCGGA 
CCCTTACCGC GGGACGGTAC TGTAGGCTGC AGCAGTGACT ACAGCAGCCC CAGTTATTCC 
TACTCCTCCA TACTCAACAA ATCAGAGACA GGTTATGTTG GTTTGGTCAA CCAAGCGATG 
ACCTGTTACC TGAACAGCCT CCTGCAGACC CTTTTCATGA CCCCTGAGTT CAGGAATGCA 
CTCTATAATT GGGAGTTTGA GGATTCTGAG GAGGATCCGG TCACCAGTAT TCCATACCAG 
CTCCAGAGGC TGTTTGTTCT CCTGCAGACC AGTAAAAAGC GTGCCATCGA GACCACAGAC 
GTGACCCGCA GCTTTGGCTG GGACAGCAGT GAGGCTTGGC AGCAGCATGA CGTCCAGGAG 
CTGTGCCGAG TCATGTTTGA TGCACTGGAG CAGAAGTGGA AACAGACTGA GCAGGCCGAC 
CTGATCAACC AGCTTTACCA GGGCAAGCTG AAGGACTATG TGCGCTGTTT GGAGTGTGGC 
TATGAGAGCT GGAGGATTGA TACGTACCTG GACATCCCTC TGGTCATCAG GCCCTTCGGC 
GCCAGCCAGG CTTTTGGCAG CGTGGAAGAA GCTCTGCAGG CCTTCATCCA GCCTGAGACT 
CTAGACGGGC CCAATCAGTA CTTCTGTGAG CGCTGCAAAA AGAAATGTGA CGCCCGGAAG 
GGTCTGAGAT TCCTTCATTT CCCATACCTG CTGACTCTGC AACTGAAACG CTTCGACTTT 
GATTACACCA CTATGCACCG CATTAAACTC AACGACCGCA TGACCTTCCC TGAGGAGCTG 
GACATGAGCC CCTTCATTGA TGTCGAGGAT GAGAAATCTC CTCAGACTGA AAGTTGCACT 
GACAGTGGGG CAGAGAATGA AGGAAGCTGC CACAGTGACC AGATGAGCAA CGATTTCTCT 
ACGGATGATG GTGTAGATGA GGGCATCTGT CTGGATAACG CCAGCAGCGC GGAGCGGGTG 
CTCAAACCAA AGAGCTCCTT GACCTTTGAA CTCTTCTCGG TCATGGTGCA TTCTGGGAGT 
GCAGCAGGTG GACACTACTA TGCCTGCATC AAGTCCTTCA GTGACAGCCA ATGGTACAGC 
TTTAATGACC AGCACGTCAG CAAGATCACG CAGGACGACA TACGGAAGAC ATATGGCGGA 
TCCTCAGGGA GCAGAGGCTA TTACTCCAGT GCCTTTGCCA GCTCTACAAA TGCATACATG 
CTGATGTACA GACTAAAAGA CCCCTCCAGG AATGCAAAGT TTCTGGAGGT GGAGGATTTT 
CCTGAGCATA TAAAGAGACT GGTGCAGCGA GAGAAAGAGT CTGAGGAGCA GGAGAAGAGG 
CAAAGAGAAA TTGAGCGCAA CACTTGCAAG ATCAAGCTGT TCTGCATGCA TCCTGTGAAG 
ATGATGATGA TGGAAAATAA ACTGGAGGTT CACAAGGACA AGACCCTCAA TGAAGCAACT 
GAAATGGCCT ATAAGCTGAT GGATTTGGAG GGAGTGGTGC CCCTGGACTG CTGTCGATTG 
GTCAAATACG ATGAGTTTCA TGAATATCTG GAACGTTCGT ATGAGGGTGA AGAAGACATG 
CCCATGGGTT TGCTGCTCGG CGGGGTCAAA TCTTCATACA TGTTTGACCT GCTCCTGGAG 
ATCCGCAGAC CCGAGCAAGC GTTCCAACCC TACAAACCTG GAGAAGTGAT GGTGAAGGTC 
CATGTGGTGG ATCTAAAGAG TGAATCTATT GCCGCACCAG TCAGTGTCAG GGCATATCTC 
AACCAGACTA TTACAGAGTT TAAGCAACTC ATTGCACAGG CAACGGGACT TTGTGCAGAG 
ACGATGCGTG TCGTCTTAGA GCGCTGCTAT AATGACCTTC GGCTTCTTTA TGTGCCCAAC 
AAGACCCTGA AAGCAGAAGG TTTCTTCAGG AGTAACAAGG TTTTCGTGGA GAGCTCCGAC 
TCTACGGATC ATCAGGCTGC CTTCACCGAC TCTGTTCTGT GGAAGCTCCT GGACCGCCAC 
GGAAACACAA TCAGACTCTT TGTCTCTCTG CCTGAGCAGT CGCCAGGCTC TTTCGCCAAT 
AGAACTAGTT GCCCCAAAGC CAGCGCTGAC TCCGAGGACT CTTTTGATGG CGCCAAGGCC 
GGCAGAAAGT CAGTAGAGGC GATCCTTGAA GAGAGCACAG AGAAGCTGAA GAACCTTTCT 
TTACAGCAAC AACAGCAGCA GCAGCAAACC TCCAGCACCA GTGACAGTCA GAAGAGCTCC 
GAACACAGCG ATTTCGAGCA CATCGAGTCA CCAGCACAGG AGCCGGATGC TTCCTCACAG 
CCTTCGCAGA CGGAGAACTG CACGCGGGCC GCTTCCGACA CGGACAACCA GTTCCCTTCT 
GAGGAGCGTT CAGACTCGGA TGTCAATAAC GACCGCAGCA CCAGCTCAGT GGACAGCGAT 
ATCCTGAGCT CCAGCCATAG CAGCGACACA CTGTGTAATG CAGACAGCGG CCCGCTGCCA 
TTGGCTAACG GATTGGACTC ACACAGCATC ACCAGCAGCC GACGCTCCAA GGCACATGAG 
GGGAAGAAGG AGACGTGGGA CACGGCAGAG GAGGACTCAG GCACGGACAG CGAGTATGAT 
GAGAACGGCA AGAGCAAAGC TGACACGCAG TACCTGTACT TCAAAGCAGA GCCGTACTCT 
CAGGAGGACG GGGCTGGAGA CGGACAGAAA AGTTTACTTG TTCACGTGGA CAAGAGGATC 
ACACTGGCAG CATTCAAACA GAATCTAGAG CCGTTTGTTG GCGTGCCCTC CACTCAGTTC 
AAGGTCTTCC GAGTTTACGC CAACAACCAG GAGTTTGAGA GCGTCAGGCT CAATGAGACG 
CTCTCCTTTT TCTCCGATGA CAACAAGATC ACCATTCGAT TGGGACGTGC TCTGAAGAAA 
GGAGAGTACA GGGTGAAGGT CTACCAGCTG CTAGTCAATG ACCCAGAGCC CTGTAAGTTC 
CTTTTGGACA CTGTGTTCGC AAAGGGCATG ACTGTTCGAC AGTCTAAGGA GGAGTTACTG 
CCTCAGCTAA AAGACCAGTG CAAACTGGAC CTCAGTATAG ACAGGTTTAG ACTCAGGAAG 
AAGACCTGGA AGAACCCAGG CACGGTGTTT CTGGATTATC ACGTCTACGA GGAGGACATC 
AACATCTCCA GTAACCTGGA GGTGTTTCTG GAGGTCCTTG AAGAACCGGA GAGGATGAAG 
TCTATGTCCC AGTTGGCGGT TCTGACCCGT CGCTGGTGTC CGGCCCAGAT GAAGCTGGAG 
GCCTTTCGGG AGGTGGTGCT GGAGAGCAGC AGCGTGGAGG AGCTCAAGGA GAAGCTCAGT 
GAAGTGAGTG GAATTCCTCT AGAAAACCTG GAGTTTGCCA AGGGACGAGG AACTTTTCCT 
TGTGACATTT CAGTGCTGGA GATCCATCAG GATTTAGACT GGAATCCAAA GGTCTCCACC 
CTTAATGTAT GGCCGCTCTA CATCTGCGAC GACGGCGCTG TGATCTTCTA CAGGGACAGC 
AGTGAGGAGC TGATGGAGCT TTCCGAAGAG GAGCGCAATG AACTGATGAA GAAGGAGAGC 
AGTCGCCTGC TGAAGACGGG CCACCGGGTC AGCTACTCGC CCCGCAAAGA GAAAGCACTC 
AAAATCTACT TGGACGGAGG GCCCACCAAA GACCCAGGAC TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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usp47 cDNA ORF clone, Danio rerio(zebrafish)

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