cfap61 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cfap61
Entrez Gene ID	100002842
Full Name	cilia and flagella associated protein 61
General protein information	Preferred Names cilia and flagella associated protein 61 Names cilia- and flagella-associated protein 61 zmp:0000000601
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294993.3	XP_009293268.2	cilia- and flagella-associated protein 61 isoform X1
XM_017352573.1	XP_017208062.1	cilia- and flagella-associated protein 61 isoform X1
XM_017352573.2	XP_017208062.1	cilia- and flagella-associated protein 61 isoform X1
XM_009294993.2	XP_009293268.2	cilia- and flagella-associated protein 61 isoform X1
XM_001342490.7	XP_001342526.6	cilia- and flagella-associated protein 61 isoform X2
XM_001342490.8	XP_001342526.6	cilia- and flagella-associated protein 61 isoform X2
XM_009294992.2	XP_009293267.2	cilia- and flagella-associated protein 61 isoform X3
XM_009294992.3	XP_009293267.2	cilia- and flagella-associated protein 61 isoform X3

Pathways from BioSystems
Gene Ontology

The following cfap61 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cfap61 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa56631	XM_009294993.3 Latest version!	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56631	XM_017352573.2 Latest version!	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56632	XM_001342490.8 Latest version!	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56633	XM_009294992.3 Latest version!	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa56631	XM_017352573.1	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56631	XM_009294993.2	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56632	XM_001342490.7	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa56633	XM_009294992.2	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cfap61 ( XM_009294993.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293268.2 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56631
Clone ID Related Accession (Same CDS sequence)	XM_009294993.3 , XM_017352573.1 , XM_017352573.2 , XM_009294993.2
Accession Version	XM_009294993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293268.2
CDS	136..3687
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_009294993.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294993.3

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_017352573.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208062.1 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56631
Clone ID Related Accession (Same CDS sequence)	XM_009294993.3 , XM_017352573.1 , XM_017352573.2 , XM_009294993.2
Accession Version	XM_017352573.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208062.1
CDS	95..3646
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_017352573.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352573.1

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_017352573.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208062.1 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.

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CloneID	ODa56631
Clone ID Related Accession (Same CDS sequence)	XM_009294993.3 , XM_017352573.1 , XM_017352573.2 , XM_009294993.2
Accession Version	XM_017352573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208062.1
CDS	141..3692
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_017352573.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352573.2

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_009294993.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293268.2 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.

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CloneID	ODa56631
Clone ID Related Accession (Same CDS sequence)	XM_009294993.3 , XM_017352573.1 , XM_017352573.2 , XM_009294993.2
Accession Version	XM_009294993.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293268.2
CDS	41..3592
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_009294993.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294993.2

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGTTA TTTTAAAGAG AACTGGTGCT TGGCTCTGTA TCATGATCGC 
TTTGCTGATT TTGAACAAGA AGTTTGTCAG ATCATGGACT CGGCTAAACT ACAGGACGAG 
AGCGGTTCAG TGTCACTTGA AGAGGCTCTT CAGCAGATCG CGGATGATAA AGCAGAGTCT 
GATCAGCTCG TGAGTCTCAG CCAGACGTTT CAGAAGAGCC AGGCTTACTC TGCTCTGAGG 
AACAGCGTTC TGGATTATCT CAAGTACAAC AGATACCATC TGAGCATGTA CGCGCGCCCG 
GGACTGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_001342490.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001342526.6 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa56632
Clone ID Related Accession (Same CDS sequence)	XM_001342490.7 , XM_001342490.8
Accession Version	XM_001342490.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001342490.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGCCCATG 
AACACTCTCT TCCTGCGGCT GTTTGTGGCT CAACCTGGCT TTTCCATAGG TGGCGCTAAA 
GAGATATTCA GGACTGTTTT CAGTGCGGTT GTGGAGCTGG AACACATCTG TCTCCTGACC 
CCATACGGAG GTGCACTTGA GGCAGCGCTG CAGAAGATCT TTGAACCCAT GACTTCTGTT 
ACGGATGAAG CTCAGTATTC AGCTTATGTT TGTCATCGAC ACGATCACTG TCCTCGACTG 
CACATCCGCA AAGCCAGAGT GGAAGATCAC GATGACCTCA TGCACATATT TGGAGAGCAG 
ATCACGCCAC TGGTGGAGAC ATACGGGCCG TTCGTCCTGT CTGAGCTCAT CGAGACACAA 
GAACAGGAGC ACCACACAGC CGTCTGTGAA AGTGACGGAG CAGTCGTTGG CTTCATCAGT 
GTGAGCGCTC ACGTGGATCT TAAGCTTCTC AACAAGTGCT TTGAACTTGG AGCATTTGAA 
GGACTGACAA AGACAGATCC ATCTCAACAA GAGGAACCGC ATCAACTGGA GGAGGAAAAC 
CCTGCCGATG ATGCTCAGCA ATCTGATGAA ACCAATGAAC CTGTGGAAGG TGAACAGACG 
GCAGAGGAAG AGACGGCAGG AGGAGACCAG TACACAGACA TATCTCAGAC AGAGTACACT 
GAGCCATGCA TTAAAGAAAC AGTGGACTGC TGTGAGGATT CTGAACCAGA CGGGCCTTCT 
AATGCATTCT GCATCCAGCT CTTCACCATG GAAAAGAAGT TTGAAATGAG ATCAGCCGAT 
TTCCTGCCGT ACCTGTTCAA ACTTTTCCCG GAGCGGGATT TCTGTGTGAT CTCCGTACCT 
AAGCAGGCTG CAGATTTCCC GCTGCTTCAG TCCTTCATCA GAGTCGCTCC TCGTGACACC 
AGCACCTTCC CGCAGGAACT CTACCTGTGT CACAGATGGG GACTGACCAG GAGACTGGAG 
GTGCGTGTGG CGGTGTCGTC AGATATCCCA GCAGTCCAGA GTTTAACCGA AAGTCTCAGC 
CAGAGAGACT CCATCATTGA AGATCTGGAT CTGTTCCTGC AGGCCCGAAA AAACTCGGAC 
GGAACTGCTC TTCAGGCATT TGTGGCTCAT GTTGATGGCC GAGTTGTTGG TTTGATAATC 
ACCAGAGACG AAGAGGACAT TGAGTTCATC CGAGCGAATT TTGACATTGA GCGGTTCATG 
TACTTCAGCC ACAACCAGCG AGAGGAACAC GGTCGACTGT GCCATTTTCT CCTCAGCCCT 
GTTTTCCAGC ATTACACCAA GTATATCTTC AGAGAGGTGC TGCGTTTGGC ACACAAGTCC 
TGTCTTTATT ATCTCCTCCC TCCACCCCAT GACTGTTATA AGAACGTCAG CGCTCACTCT 
CTGACAGCCG TGCTGAACTT CATGGTGCCT GTGAGTCCGA GACGACAGAT AATATACCCG 
CTGGAGGAGC TGGGCATCAA TGCACCATCC CAGCATATCA CCAAACTACA GGCTCCTTTT 
GCGCTGTACC ACACAAACAG AAAACTGGCC ATGGAGCCGA AGGTCACCAT CAATGCCAGA 
ATCGTGGTGG TGGGAGCGTC AGACACGGCT TTAGCGTTCC TCGAAGCTCT AACATTCTGT 
CCTCATCTGA GATTCAACAA CCTGAGCCTC ATCTCCACTC ACGCTCTCCC AGATCACAGC 
TCTGATGACT GCAGCACATT TCTGGCCACG AGTCACTGCT ATAATGATCG GGATCAGTGT 
CAGATTTCTC TGTGCTCCTG GATCAGCGTC GTGACAGGGA AGATAAAAGC CATTAACCGA 
GCGGACAAAC ACGTGGAGCT GATGGACGAC AGCAGAGTGA AGTTCGACTA TCTGATCCTC 
TGCACCGGCC TGCAGTATCA GATGCCCAAT CTCTCCAATG CTGATGTCAT TACCCAAACC 
TCCAACAGTC TGAAACCTAA AGAAACCCGA CCCAGAGTCC CATCAAACCT CTTCCTCCTC 
AACGACCATC AGGATTGCTC CGTCGCCCAT CACTGGCTCA CCCACAACTT CACACAGCTA 
ACAGGTGATG CTGTGGTGTA TGGAAACACT ATAGACGTGT TCACCTGCAT AGAGACTCTC 
ATTGGTCTCG GTGTCAGTGG CCGCAGGATT CATGTAGTCC ATCCTCCTGA AGATCAACCA 
TTCTCCTGTT TCCCCGACGC TTCAGTGGAG CTGGCAGTGA AACAGGCTCT AGAAAAAGAG 
GAAGTCCATG TTCATCACAA CTGCCTGTTG CTGCAGATCA ATGATGGACA ACACACAGAG 
CCTGTGACAT CTGCCTCTTT CAGCACGGAT GGAGCCATTC TACAGCTGGA GTGTGCGGTG 
TTCTTCAGCT TCTCTCGTAA AAGCGTGGAC TGTGACGCTT TCAGGGCCAT CAATGATGCG 
TGTTTGGTGT TTGATGGGCG ATTGGTTGTA GACAGCGGCT TTCACACCAA TGACCCCTCC 
ATATACGCCG CAGGCCCCAT GACCAAATAC TCCCGCCGCT ACCATGCAGA AGAGTGGAGC 
CATTGCTGCT TTCAATCCAA AGAGGTGGGC CAAAGTTTGG CATCTGTCCT CCTGGCCCAG 
TGTGACCCGA CTCTGGAGCG TCCCGTCGAC CCTCCGCCTG ACCAGCTCCA CCTCATTCCA 
CTCTACAGCC AGGCCAAGAC ACAAGGGGGA AGGTTACCAG GAGGATACAA TTACCTTCAT 
GTGGCAAAAC CAGTCGCCAG AAAAACTCCA TCAGCTCACA AAGGTCGTGA TCTGGTGACG 
GGCTGTGCTG AAACCGGGAA CTACTTTCAC ATACGCCACA GTCAGTTTGA CGTGGTGGAG 
AAAATCACGT GTTTGTCCAA GACTCCACTT CCTGTCTCCA ACCTCCAGAG TCTCTACGGC 
AAACACCAGC GGCTGCTCAA CAACATCTGC TCACGATACG ATGAGGGATT GATCCAGGAC 
ATGTACAGTT ATTTTAAAGA GAACTGGTGC TTGGCTCTGT ATCATGATCG CTTTGCTGAT 
TTTGAACAAG AAGTTTGTCA GATCATGGAC TCGGCTAAAC TACAGGACGA GAGCGGTTCA 
GTGTCACTTG AAGAGGCTCT TCAGCAGATC GCGGATGATA AAGCAGAGTC TGATCAGCTC 
GTGAGTCTCA GCCAGACGTT TCAGAAGAGC CAGGCTTACT CTGCTCTGAG GAACAGCGTT 
CTGGATTATC TCAAGTACAA CAGATACCAT CTGAGCATGT ACGCGCGCCC GGGACTGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001342526.6
CDS	95..3637
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_001342490.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001342490.7

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGCCCATG 
AACACTCTCT TCCTGCGGCT GTTTGTGGCT CAACCTGGCT TTTCCATAGG TGGCGCTAAA 
GAGATATTCA GGACTGTTTT CAGTGCGGTT GTGGAGCTGG AACACATCTG TCTCCTGACC 
CCATACGGAG GTGCACTTGA GGCAGCGCTG CAGAAGATCT TTGAACCCAT GACTTCTGTT 
ACGGATGAAG CTCAGTATTC AGCTTATGTT TGTCATCGAC ACGATCACTG TCCTCGACTG 
CACATCCGCA AAGCCAGAGT GGAAGATCAC GATGACCTCA TGCACATATT TGGAGAGCAG 
ATCACGCCAC TGGTGGAGAC ATACGGGCCG TTCGTCCTGT CTGAGCTCAT CGAGACACAA 
GAACAGGAGC ACCACACAGC CGTCTGTGAA AGTGACGGAG CAGTCGTTGG CTTCATCAGT 
GTGAGCGCTC ACGTGGATCT TAAGCTTCTC AACAAGTGCT TTGAACTTGG AGCATTTGAA 
GGACTGACAA AGACAGATCC ATCTCAACAA GAGGAACCGC ATCAACTGGA GGAGGAAAAC 
CCTGCCGATG ATGCTCAGCA ATCTGATGAA ACCAATGAAC CTGTGGAAGG TGAACAGACG 
GCAGAGGAAG AGACGGCAGG AGGAGACCAG TACACAGACA TATCTCAGAC AGAGTACACT 
GAGCCATGCA TTAAAGAAAC AGTGGACTGC TGTGAGGATT CTGAACCAGA CGGGCCTTCT 
AATGCATTCT GCATCCAGCT CTTCACCATG GAAAAGAAGT TTGAAATGAG ATCAGCCGAT 
TTCCTGCCGT ACCTGTTCAA ACTTTTCCCG GAGCGGGATT TCTGTGTGAT CTCCGTACCT 
AAGCAGGCTG CAGATTTCCC GCTGCTTCAG TCCTTCATCA GAGTCGCTCC TCGTGACACC 
AGCACCTTCC CGCAGGAACT CTACCTGTGT CACAGATGGG GACTGACCAG GAGACTGGAG 
GTGCGTGTGG CGGTGTCGTC AGATATCCCA GCAGTCCAGA GTTTAACCGA AAGTCTCAGC 
CAGAGAGACT CCATCATTGA AGATCTGGAT CTGTTCCTGC AGGCCCGAAA AAACTCGGAC 
GGAACTGCTC TTCAGGCATT TGTGGCTCAT GTTGATGGCC GAGTTGTTGG TTTGATAATC 
ACCAGAGACG AAGAGGACAT TGAGTTCATC CGAGCGAATT TTGACATTGA GCGGTTCATG 
TACTTCAGCC ACAACCAGCG AGAGGAACAC GGTCGACTGT GCCATTTTCT CCTCAGCCCT 
GTTTTCCAGC ATTACACCAA GTATATCTTC AGAGAGGTGC TGCGTTTGGC ACACAAGTCC 
TGTCTTTATT ATCTCCTCCC TCCACCCCAT GACTGTTATA AGAACGTCAG CGCTCACTCT 
CTGACAGCCG TGCTGAACTT CATGGTGCCT GTGAGTCCGA GACGACAGAT AATATACCCG 
CTGGAGGAGC TGGGCATCAA TGCACCATCC CAGCATATCA CCAAACTACA GGCTCCTTTT 
GCGCTGTACC ACACAAACAG AAAACTGGCC ATGGAGCCGA AGGTCACCAT CAATGCCAGA 
ATCGTGGTGG TGGGAGCGTC AGACACGGCT TTAGCGTTCC TCGAAGCTCT AACATTCTGT 
CCTCATCTGA GATTCAACAA CCTGAGCCTC ATCTCCACTC ACGCTCTCCC AGATCACAGC 
TCTGATGACT GCAGCACATT TCTGGCCACG AGTCACTGCT ATAATGATCG GGATCAGTGT 
CAGATTTCTC TGTGCTCCTG GATCAGCGTC GTGACAGGGA AGATAAAAGC CATTAACCGA 
GCGGACAAAC ACGTGGAGCT GATGGACGAC AGCAGAGTGA AGTTCGACTA TCTGATCCTC 
TGCACCGGCC TGCAGTATCA GATGCCCAAT CTCTCCAATG CTGATGTCAT TACCCAAACC 
TCCAACAGTC TGAAACCTAA AGAAACCCGA CCCAGAGTCC CATCAAACCT CTTCCTCCTC 
AACGACCATC AGGATTGCTC CGTCGCCCAT CACTGGCTCA CCCACAACTT CACACAGCTA 
ACAGGTGATG CTGTGGTGTA TGGAAACACT ATAGACGTGT TCACCTGCAT AGAGACTCTC 
ATTGGTCTCG GTGTCAGTGG CCGCAGGATT CATGTAGTCC ATCCTCCTGA AGATCAACCA 
TTCTCCTGTT TCCCCGACGC TTCAGTGGAG CTGGCAGTGA AACAGGCTCT AGAAAAAGAG 
GAAGTCCATG TTCATCACAA CTGCCTGTTG CTGCAGATCA ATGATGGACA ACACACAGAG 
CCTGTGACAT CTGCCTCTTT CAGCACGGAT GGAGCCATTC TACAGCTGGA GTGTGCGGTG 
TTCTTCAGCT TCTCTCGTAA AAGCGTGGAC TGTGACGCTT TCAGGGCCAT CAATGATGCG 
TGTTTGGTGT TTGATGGGCG ATTGGTTGTA GACAGCGGCT TTCACACCAA TGACCCCTCC 
ATATACGCCG CAGGCCCCAT GACCAAATAC TCCCGCCGCT ACCATGCAGA AGAGTGGAGC 
CATTGCTGCT TTCAATCCAA AGAGGTGGGC CAAAGTTTGG CATCTGTCCT CCTGGCCCAG 
TGTGACCCGA CTCTGGAGCG TCCCGTCGAC CCTCCGCCTG ACCAGCTCCA CCTCATTCCA 
CTCTACAGCC AGGCCAAGAC ACAAGGGGGA AGGTTACCAG GAGGATACAA TTACCTTCAT 
GTGGCAAAAC CAGTCGCCAG AAAAACTCCA TCAGCTCACA AAGGTCGTGA TCTGGTGACG 
GGCTGTGCTG AAACCGGGAA CTACTTTCAC ATACGCCACA GTCAGTTTGA CGTGGTGGAG 
AAAATCACGT GTTTGTCCAA GACTCCACTT CCTGTCTCCA ACCTCCAGAG TCTCTACGGC 
AAACACCAGC GGCTGCTCAA CAACATCTGC TCACGATACG ATGAGGGATT GATCCAGGAC 
ATGTACAGTT ATTTTAAAGA GAACTGGTGC TTGGCTCTGT ATCATGATCG CTTTGCTGAT 
TTTGAACAAG AAGTTTGTCA GATCATGGAC TCGGCTAAAC TACAGGACGA GAGCGGTTCA 
GTGTCACTTG AAGAGGCTCT TCAGCAGATC GCGGATGATA AAGCAGAGTC TGATCAGCTC 
GTGAGTCTCA GCCAGACGTT TCAGAAGAGC CAGGCTTACT CTGCTCTGAG GAACAGCGTT 
CTGGATTATC TCAAGTACAA CAGATACCAT CTGAGCATGT ACGCGCGCCC GGGACTGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_001342490.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001342526.6 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.

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CloneID	ODa56632
Clone ID Related Accession (Same CDS sequence)	XM_001342490.7 , XM_001342490.8
Accession Version	XM_001342490.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001342490.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGCCCATG 
AACACTCTCT TCCTGCGGCT GTTTGTGGCT CAACCTGGCT TTTCCATAGG TGGCGCTAAA 
GAGATATTCA GGACTGTTTT CAGTGCGGTT GTGGAGCTGG AACACATCTG TCTCCTGACC 
CCATACGGAG GTGCACTTGA GGCAGCGCTG CAGAAGATCT TTGAACCCAT GACTTCTGTT 
ACGGATGAAG CTCAGTATTC AGCTTATGTT TGTCATCGAC ACGATCACTG TCCTCGACTG 
CACATCCGCA AAGCCAGAGT GGAAGATCAC GATGACCTCA TGCACATATT TGGAGAGCAG 
ATCACGCCAC TGGTGGAGAC ATACGGGCCG TTCGTCCTGT CTGAGCTCAT CGAGACACAA 
GAACAGGAGC ACCACACAGC CGTCTGTGAA AGTGACGGAG CAGTCGTTGG CTTCATCAGT 
GTGAGCGCTC ACGTGGATCT TAAGCTTCTC AACAAGTGCT TTGAACTTGG AGCATTTGAA 
GGACTGACAA AGACAGATCC ATCTCAACAA GAGGAACCGC ATCAACTGGA GGAGGAAAAC 
CCTGCCGATG ATGCTCAGCA ATCTGATGAA ACCAATGAAC CTGTGGAAGG TGAACAGACG 
GCAGAGGAAG AGACGGCAGG AGGAGACCAG TACACAGACA TATCTCAGAC AGAGTACACT 
GAGCCATGCA TTAAAGAAAC AGTGGACTGC TGTGAGGATT CTGAACCAGA CGGGCCTTCT 
AATGCATTCT GCATCCAGCT CTTCACCATG GAAAAGAAGT TTGAAATGAG ATCAGCCGAT 
TTCCTGCCGT ACCTGTTCAA ACTTTTCCCG GAGCGGGATT TCTGTGTGAT CTCCGTACCT 
AAGCAGGCTG CAGATTTCCC GCTGCTTCAG TCCTTCATCA GAGTCGCTCC TCGTGACACC 
AGCACCTTCC CGCAGGAACT CTACCTGTGT CACAGATGGG GACTGACCAG GAGACTGGAG 
GTGCGTGTGG CGGTGTCGTC AGATATCCCA GCAGTCCAGA GTTTAACCGA AAGTCTCAGC 
CAGAGAGACT CCATCATTGA AGATCTGGAT CTGTTCCTGC AGGCCCGAAA AAACTCGGAC 
GGAACTGCTC TTCAGGCATT TGTGGCTCAT GTTGATGGCC GAGTTGTTGG TTTGATAATC 
ACCAGAGACG AAGAGGACAT TGAGTTCATC CGAGCGAATT TTGACATTGA GCGGTTCATG 
TACTTCAGCC ACAACCAGCG AGAGGAACAC GGTCGACTGT GCCATTTTCT CCTCAGCCCT 
GTTTTCCAGC ATTACACCAA GTATATCTTC AGAGAGGTGC TGCGTTTGGC ACACAAGTCC 
TGTCTTTATT ATCTCCTCCC TCCACCCCAT GACTGTTATA AGAACGTCAG CGCTCACTCT 
CTGACAGCCG TGCTGAACTT CATGGTGCCT GTGAGTCCGA GACGACAGAT AATATACCCG 
CTGGAGGAGC TGGGCATCAA TGCACCATCC CAGCATATCA CCAAACTACA GGCTCCTTTT 
GCGCTGTACC ACACAAACAG AAAACTGGCC ATGGAGCCGA AGGTCACCAT CAATGCCAGA 
ATCGTGGTGG TGGGAGCGTC AGACACGGCT TTAGCGTTCC TCGAAGCTCT AACATTCTGT 
CCTCATCTGA GATTCAACAA CCTGAGCCTC ATCTCCACTC ACGCTCTCCC AGATCACAGC 
TCTGATGACT GCAGCACATT TCTGGCCACG AGTCACTGCT ATAATGATCG GGATCAGTGT 
CAGATTTCTC TGTGCTCCTG GATCAGCGTC GTGACAGGGA AGATAAAAGC CATTAACCGA 
GCGGACAAAC ACGTGGAGCT GATGGACGAC AGCAGAGTGA AGTTCGACTA TCTGATCCTC 
TGCACCGGCC TGCAGTATCA GATGCCCAAT CTCTCCAATG CTGATGTCAT TACCCAAACC 
TCCAACAGTC TGAAACCTAA AGAAACCCGA CCCAGAGTCC CATCAAACCT CTTCCTCCTC 
AACGACCATC AGGATTGCTC CGTCGCCCAT CACTGGCTCA CCCACAACTT CACACAGCTA 
ACAGGTGATG CTGTGGTGTA TGGAAACACT ATAGACGTGT TCACCTGCAT AGAGACTCTC 
ATTGGTCTCG GTGTCAGTGG CCGCAGGATT CATGTAGTCC ATCCTCCTGA AGATCAACCA 
TTCTCCTGTT TCCCCGACGC TTCAGTGGAG CTGGCAGTGA AACAGGCTCT AGAAAAAGAG 
GAAGTCCATG TTCATCACAA CTGCCTGTTG CTGCAGATCA ATGATGGACA ACACACAGAG 
CCTGTGACAT CTGCCTCTTT CAGCACGGAT GGAGCCATTC TACAGCTGGA GTGTGCGGTG 
TTCTTCAGCT TCTCTCGTAA AAGCGTGGAC TGTGACGCTT TCAGGGCCAT CAATGATGCG 
TGTTTGGTGT TTGATGGGCG ATTGGTTGTA GACAGCGGCT TTCACACCAA TGACCCCTCC 
ATATACGCCG CAGGCCCCAT GACCAAATAC TCCCGCCGCT ACCATGCAGA AGAGTGGAGC 
CATTGCTGCT TTCAATCCAA AGAGGTGGGC CAAAGTTTGG CATCTGTCCT CCTGGCCCAG 
TGTGACCCGA CTCTGGAGCG TCCCGTCGAC CCTCCGCCTG ACCAGCTCCA CCTCATTCCA 
CTCTACAGCC AGGCCAAGAC ACAAGGGGGA AGGTTACCAG GAGGATACAA TTACCTTCAT 
GTGGCAAAAC CAGTCGCCAG AAAAACTCCA TCAGCTCACA AAGGTCGTGA TCTGGTGACG 
GGCTGTGCTG AAACCGGGAA CTACTTTCAC ATACGCCACA GTCAGTTTGA CGTGGTGGAG 
AAAATCACGT GTTTGTCCAA GACTCCACTT CCTGTCTCCA ACCTCCAGAG TCTCTACGGC 
AAACACCAGC GGCTGCTCAA CAACATCTGC TCACGATACG ATGAGGGATT GATCCAGGAC 
ATGTACAGTT ATTTTAAAGA GAACTGGTGC TTGGCTCTGT ATCATGATCG CTTTGCTGAT 
TTTGAACAAG AAGTTTGTCA GATCATGGAC TCGGCTAAAC TACAGGACGA GAGCGGTTCA 
GTGTCACTTG AAGAGGCTCT TCAGCAGATC GCGGATGATA AAGCAGAGTC TGATCAGCTC 
GTGAGTCTCA GCCAGACGTT TCAGAAGAGC CAGGCTTACT CTGCTCTGAG GAACAGCGTT 
CTGGATTATC TCAAGTACAA CAGATACCAT CTGAGCATGT ACGCGCGCCC GGGACTGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001342526.6
CDS	141..3683
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYSYFKENWCLALYHDRFADFEQEVCQIMDSAKLQDESGSVSLEEALQQIADDKAESDQLVSLSQTFQKSQAYSALRNSVLDYLKYNRYHLSMYARPGLL

Target ORF information:

RefSeq Version	XM_001342490.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001342490.8

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGCCCATG 
AACACTCTCT TCCTGCGGCT GTTTGTGGCT CAACCTGGCT TTTCCATAGG TGGCGCTAAA 
GAGATATTCA GGACTGTTTT CAGTGCGGTT GTGGAGCTGG AACACATCTG TCTCCTGACC 
CCATACGGAG GTGCACTTGA GGCAGCGCTG CAGAAGATCT TTGAACCCAT GACTTCTGTT 
ACGGATGAAG CTCAGTATTC AGCTTATGTT TGTCATCGAC ACGATCACTG TCCTCGACTG 
CACATCCGCA AAGCCAGAGT GGAAGATCAC GATGACCTCA TGCACATATT TGGAGAGCAG 
ATCACGCCAC TGGTGGAGAC ATACGGGCCG TTCGTCCTGT CTGAGCTCAT CGAGACACAA 
GAACAGGAGC ACCACACAGC CGTCTGTGAA AGTGACGGAG CAGTCGTTGG CTTCATCAGT 
GTGAGCGCTC ACGTGGATCT TAAGCTTCTC AACAAGTGCT TTGAACTTGG AGCATTTGAA 
GGACTGACAA AGACAGATCC ATCTCAACAA GAGGAACCGC ATCAACTGGA GGAGGAAAAC 
CCTGCCGATG ATGCTCAGCA ATCTGATGAA ACCAATGAAC CTGTGGAAGG TGAACAGACG 
GCAGAGGAAG AGACGGCAGG AGGAGACCAG TACACAGACA TATCTCAGAC AGAGTACACT 
GAGCCATGCA TTAAAGAAAC AGTGGACTGC TGTGAGGATT CTGAACCAGA CGGGCCTTCT 
AATGCATTCT GCATCCAGCT CTTCACCATG GAAAAGAAGT TTGAAATGAG ATCAGCCGAT 
TTCCTGCCGT ACCTGTTCAA ACTTTTCCCG GAGCGGGATT TCTGTGTGAT CTCCGTACCT 
AAGCAGGCTG CAGATTTCCC GCTGCTTCAG TCCTTCATCA GAGTCGCTCC TCGTGACACC 
AGCACCTTCC CGCAGGAACT CTACCTGTGT CACAGATGGG GACTGACCAG GAGACTGGAG 
GTGCGTGTGG CGGTGTCGTC AGATATCCCA GCAGTCCAGA GTTTAACCGA AAGTCTCAGC 
CAGAGAGACT CCATCATTGA AGATCTGGAT CTGTTCCTGC AGGCCCGAAA AAACTCGGAC 
GGAACTGCTC TTCAGGCATT TGTGGCTCAT GTTGATGGCC GAGTTGTTGG TTTGATAATC 
ACCAGAGACG AAGAGGACAT TGAGTTCATC CGAGCGAATT TTGACATTGA GCGGTTCATG 
TACTTCAGCC ACAACCAGCG AGAGGAACAC GGTCGACTGT GCCATTTTCT CCTCAGCCCT 
GTTTTCCAGC ATTACACCAA GTATATCTTC AGAGAGGTGC TGCGTTTGGC ACACAAGTCC 
TGTCTTTATT ATCTCCTCCC TCCACCCCAT GACTGTTATA AGAACGTCAG CGCTCACTCT 
CTGACAGCCG TGCTGAACTT CATGGTGCCT GTGAGTCCGA GACGACAGAT AATATACCCG 
CTGGAGGAGC TGGGCATCAA TGCACCATCC CAGCATATCA CCAAACTACA GGCTCCTTTT 
GCGCTGTACC ACACAAACAG AAAACTGGCC ATGGAGCCGA AGGTCACCAT CAATGCCAGA 
ATCGTGGTGG TGGGAGCGTC AGACACGGCT TTAGCGTTCC TCGAAGCTCT AACATTCTGT 
CCTCATCTGA GATTCAACAA CCTGAGCCTC ATCTCCACTC ACGCTCTCCC AGATCACAGC 
TCTGATGACT GCAGCACATT TCTGGCCACG AGTCACTGCT ATAATGATCG GGATCAGTGT 
CAGATTTCTC TGTGCTCCTG GATCAGCGTC GTGACAGGGA AGATAAAAGC CATTAACCGA 
GCGGACAAAC ACGTGGAGCT GATGGACGAC AGCAGAGTGA AGTTCGACTA TCTGATCCTC 
TGCACCGGCC TGCAGTATCA GATGCCCAAT CTCTCCAATG CTGATGTCAT TACCCAAACC 
TCCAACAGTC TGAAACCTAA AGAAACCCGA CCCAGAGTCC CATCAAACCT CTTCCTCCTC 
AACGACCATC AGGATTGCTC CGTCGCCCAT CACTGGCTCA CCCACAACTT CACACAGCTA 
ACAGGTGATG CTGTGGTGTA TGGAAACACT ATAGACGTGT TCACCTGCAT AGAGACTCTC 
ATTGGTCTCG GTGTCAGTGG CCGCAGGATT CATGTAGTCC ATCCTCCTGA AGATCAACCA 
TTCTCCTGTT TCCCCGACGC TTCAGTGGAG CTGGCAGTGA AACAGGCTCT AGAAAAAGAG 
GAAGTCCATG TTCATCACAA CTGCCTGTTG CTGCAGATCA ATGATGGACA ACACACAGAG 
CCTGTGACAT CTGCCTCTTT CAGCACGGAT GGAGCCATTC TACAGCTGGA GTGTGCGGTG 
TTCTTCAGCT TCTCTCGTAA AAGCGTGGAC TGTGACGCTT TCAGGGCCAT CAATGATGCG 
TGTTTGGTGT TTGATGGGCG ATTGGTTGTA GACAGCGGCT TTCACACCAA TGACCCCTCC 
ATATACGCCG CAGGCCCCAT GACCAAATAC TCCCGCCGCT ACCATGCAGA AGAGTGGAGC 
CATTGCTGCT TTCAATCCAA AGAGGTGGGC CAAAGTTTGG CATCTGTCCT CCTGGCCCAG 
TGTGACCCGA CTCTGGAGCG TCCCGTCGAC CCTCCGCCTG ACCAGCTCCA CCTCATTCCA 
CTCTACAGCC AGGCCAAGAC ACAAGGGGGA AGGTTACCAG GAGGATACAA TTACCTTCAT 
GTGGCAAAAC CAGTCGCCAG AAAAACTCCA TCAGCTCACA AAGGTCGTGA TCTGGTGACG 
GGCTGTGCTG AAACCGGGAA CTACTTTCAC ATACGCCACA GTCAGTTTGA CGTGGTGGAG 
AAAATCACGT GTTTGTCCAA GACTCCACTT CCTGTCTCCA ACCTCCAGAG TCTCTACGGC 
AAACACCAGC GGCTGCTCAA CAACATCTGC TCACGATACG ATGAGGGATT GATCCAGGAC 
ATGTACAGTT ATTTTAAAGA GAACTGGTGC TTGGCTCTGT ATCATGATCG CTTTGCTGAT 
TTTGAACAAG AAGTTTGTCA GATCATGGAC TCGGCTAAAC TACAGGACGA GAGCGGTTCA 
GTGTCACTTG AAGAGGCTCT TCAGCAGATC GCGGATGATA AAGCAGAGTC TGATCAGCTC 
GTGAGTCTCA GCCAGACGTT TCAGAAGAGC CAGGCTTACT CTGCTCTGAG GAACAGCGTT 
CTGGATTATC TCAAGTACAA CAGATACCAT CTGAGCATGT ACGCGCGCCC GGGACTGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_009294992.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293267.2 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.

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CloneID	ODa56633
Clone ID Related Accession (Same CDS sequence)	XM_009294992.2 , XM_009294992.3
Accession Version	XM_009294992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGGCC TGATTACTAC CACAACTGTT CGCAACCAAG ACATCACTTA 
AATAGGACCT GCCTGACAAA AGGAAGTAGA CCAAAAGATA CTCATAATAT ATATATGCAA 
CTACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293267.2
CDS	95..3463
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYRPDYYHNCSQPRHHLNRTCLTKGSRPKDTHNIYMQLL

Target ORF information:

RefSeq Version	XM_009294992.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294992.2

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGGCC TGATTACTAC CACAACTGTT CGCAACCAAG ACATCACTTA 
AATAGGACCT GCCTGACAAA AGGAAGTAGA CCAAAAGATA CTCATAATAT ATATATGCAA 
CTACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cfap61 ( XM_009294992.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293267.2 Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.

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CloneID	ODa56633
Clone ID Related Accession (Same CDS sequence)	XM_009294992.2 , XM_009294992.3
Accession Version	XM_009294992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cilia- and flagella-associated protein 61 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGGCC TGATTACTAC CACAACTGTT CGCAACCAAG ACATCACTTA 
AATAGGACCT GCCTGACAAA AGGAAGTAGA CCAAAAGATA CTCATAATAT ATATATGCAA 
CTACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293267.2
CDS	141..3509
Translation	MSTITSSTGKAESVTVRRTEWSDAEEINHLISPASVAVFGRLNVINLLEKANLAVTLSSSKNEVLAHASFSDHPIEELVDQACWQNRLQNYASAEKLTVGRPMNTLFLRLFVAQPGFSIGGAKEIFRTVFSAVVELEHICLLTPYGGALEAALQKIFEPMTSVTDEAQYSAYVCHRHDHCPRLHIRKARVEDHDDLMHIFGEQITPLVETYGPFVLSELIETQEQEHHTAVCESDGAVVGFISVSAHVDLKLLNKCFELGAFEGLTKTDPSQQEEPHQLEEENPADDAQQSDETNEPVEGEQTAEEETAGGDQYTDISQTEYTEPCIKETVDCCEDSEPDGPSNAFCIQLFTMEKKFEMRSADFLPYLFKLFPERDFCVISVPKQAADFPLLQSFIRVAPRDTSTFPQELYLCHRWGLTRRLEVRVAVSSDIPAVQSLTESLSQRDSIIEDLDLFLQARKNSDGTALQAFVAHVDGRVVGLIITRDEEDIEFIRANFDIERFMYFSHNQREEHGRLCHFLLSPVFQHYTKYIFREVLRLAHKSCLYYLLPPPHDCYKNVSAHSLTAVLNFMVPVSPRRQIIYPLEELGINAPSQHITKLQAPFALYHTNRKLAMEPKVTINARIVVVGASDTALAFLEALTFCPHLRFNNLSLISTHALPDHSSDDCSTFLATSHCYNDRDQCQISLCSWISVVTGKIKAINRADKHVELMDDSRVKFDYLILCTGLQYQMPNLSNADVITQTSNSLKPKETRPRVPSNLFLLNDHQDCSVAHHWLTHNFTQLTGDAVVYGNTIDVFTCIETLIGLGVSGRRIHVVHPPEDQPFSCFPDASVELAVKQALEKEEVHVHHNCLLLQINDGQHTEPVTSASFSTDGAILQLECAVFFSFSRKSVDCDAFRAINDACLVFDGRLVVDSGFHTNDPSIYAAGPMTKYSRRYHAEEWSHCCFQSKEVGQSLASVLLAQCDPTLERPVDPPPDQLHLIPLYSQAKTQGGRLPGGYNYLHVAKPVARKTPSAHKGRDLVTGCAETGNYFHIRHSQFDVVEKITCLSKTPLPVSNLQSLYGKHQRLLNNICSRYDEGLIQDMYRPDYYHNCSQPRHHLNRTCLTKGSRPKDTHNIYMQLL

Target ORF information:

RefSeq Version	XM_009294992.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cilia and flagella associated protein 61 (cfap61), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294992.3

ORF Insert Sequence:

ATGAGCACCA TCACTTCATC CACTGGTAAA GCAGAAAGTG TGACCGTGAG GAGAACTGAA 
TGGTCAGATG CTGAAGAGAT CAATCATCTG ATCAGTCCGG CATCTGTTGC CGTGTTCGGG 
AGACTTAATG TCATCAATCT CCTTGAGAAA GCAAATTTAG CCGTGACCCT GAGCTCCAGT 
AAGAATGAAG TTTTGGCCCA CGCATCTTTC TCAGATCACC CAATAGAAGA GCTGGTGGAT 
CAGGCATGCT GGCAGAATCG GCTTCAAAAT TACGCCAGTG CAGAGAAATT GACGGTAGGA 
AGACCCATGA ACACTCTCTT CCTGCGGCTG TTTGTGGCTC AACCTGGCTT TTCCATAGGT 
GGCGCTAAAG AGATATTCAG GACTGTTTTC AGTGCGGTTG TGGAGCTGGA ACACATCTGT 
CTCCTGACCC CATACGGAGG TGCACTTGAG GCAGCGCTGC AGAAGATCTT TGAACCCATG 
ACTTCTGTTA CGGATGAAGC TCAGTATTCA GCTTATGTTT GTCATCGACA CGATCACTGT 
CCTCGACTGC ACATCCGCAA AGCCAGAGTG GAAGATCACG ATGACCTCAT GCACATATTT 
GGAGAGCAGA TCACGCCACT GGTGGAGACA TACGGGCCGT TCGTCCTGTC TGAGCTCATC 
GAGACACAAG AACAGGAGCA CCACACAGCC GTCTGTGAAA GTGACGGAGC AGTCGTTGGC 
TTCATCAGTG TGAGCGCTCA CGTGGATCTT AAGCTTCTCA ACAAGTGCTT TGAACTTGGA 
GCATTTGAAG GACTGACAAA GACAGATCCA TCTCAACAAG AGGAACCGCA TCAACTGGAG 
GAGGAAAACC CTGCCGATGA TGCTCAGCAA TCTGATGAAA CCAATGAACC TGTGGAAGGT 
GAACAGACGG CAGAGGAAGA GACGGCAGGA GGAGACCAGT ACACAGACAT ATCTCAGACA 
GAGTACACTG AGCCATGCAT TAAAGAAACA GTGGACTGCT GTGAGGATTC TGAACCAGAC 
GGGCCTTCTA ATGCATTCTG CATCCAGCTC TTCACCATGG AAAAGAAGTT TGAAATGAGA 
TCAGCCGATT TCCTGCCGTA CCTGTTCAAA CTTTTCCCGG AGCGGGATTT CTGTGTGATC 
TCCGTACCTA AGCAGGCTGC AGATTTCCCG CTGCTTCAGT CCTTCATCAG AGTCGCTCCT 
CGTGACACCA GCACCTTCCC GCAGGAACTC TACCTGTGTC ACAGATGGGG ACTGACCAGG 
AGACTGGAGG TGCGTGTGGC GGTGTCGTCA GATATCCCAG CAGTCCAGAG TTTAACCGAA 
AGTCTCAGCC AGAGAGACTC CATCATTGAA GATCTGGATC TGTTCCTGCA GGCCCGAAAA 
AACTCGGACG GAACTGCTCT TCAGGCATTT GTGGCTCATG TTGATGGCCG AGTTGTTGGT 
TTGATAATCA CCAGAGACGA AGAGGACATT GAGTTCATCC GAGCGAATTT TGACATTGAG 
CGGTTCATGT ACTTCAGCCA CAACCAGCGA GAGGAACACG GTCGACTGTG CCATTTTCTC 
CTCAGCCCTG TTTTCCAGCA TTACACCAAG TATATCTTCA GAGAGGTGCT GCGTTTGGCA 
CACAAGTCCT GTCTTTATTA TCTCCTCCCT CCACCCCATG ACTGTTATAA GAACGTCAGC 
GCTCACTCTC TGACAGCCGT GCTGAACTTC ATGGTGCCTG TGAGTCCGAG ACGACAGATA 
ATATACCCGC TGGAGGAGCT GGGCATCAAT GCACCATCCC AGCATATCAC CAAACTACAG 
GCTCCTTTTG CGCTGTACCA CACAAACAGA AAACTGGCCA TGGAGCCGAA GGTCACCATC 
AATGCCAGAA TCGTGGTGGT GGGAGCGTCA GACACGGCTT TAGCGTTCCT CGAAGCTCTA 
ACATTCTGTC CTCATCTGAG ATTCAACAAC CTGAGCCTCA TCTCCACTCA CGCTCTCCCA 
GATCACAGCT CTGATGACTG CAGCACATTT CTGGCCACGA GTCACTGCTA TAATGATCGG 
GATCAGTGTC AGATTTCTCT GTGCTCCTGG ATCAGCGTCG TGACAGGGAA GATAAAAGCC 
ATTAACCGAG CGGACAAACA CGTGGAGCTG ATGGACGACA GCAGAGTGAA GTTCGACTAT 
CTGATCCTCT GCACCGGCCT GCAGTATCAG ATGCCCAATC TCTCCAATGC TGATGTCATT 
ACCCAAACCT CCAACAGTCT GAAACCTAAA GAAACCCGAC CCAGAGTCCC ATCAAACCTC 
TTCCTCCTCA ACGACCATCA GGATTGCTCC GTCGCCCATC ACTGGCTCAC CCACAACTTC 
ACACAGCTAA CAGGTGATGC TGTGGTGTAT GGAAACACTA TAGACGTGTT CACCTGCATA 
GAGACTCTCA TTGGTCTCGG TGTCAGTGGC CGCAGGATTC ATGTAGTCCA TCCTCCTGAA 
GATCAACCAT TCTCCTGTTT CCCCGACGCT TCAGTGGAGC TGGCAGTGAA ACAGGCTCTA 
GAAAAAGAGG AAGTCCATGT TCATCACAAC TGCCTGTTGC TGCAGATCAA TGATGGACAA 
CACACAGAGC CTGTGACATC TGCCTCTTTC AGCACGGATG GAGCCATTCT ACAGCTGGAG 
TGTGCGGTGT TCTTCAGCTT CTCTCGTAAA AGCGTGGACT GTGACGCTTT CAGGGCCATC 
AATGATGCGT GTTTGGTGTT TGATGGGCGA TTGGTTGTAG ACAGCGGCTT TCACACCAAT 
GACCCCTCCA TATACGCCGC AGGCCCCATG ACCAAATACT CCCGCCGCTA CCATGCAGAA 
GAGTGGAGCC ATTGCTGCTT TCAATCCAAA GAGGTGGGCC AAAGTTTGGC ATCTGTCCTC 
CTGGCCCAGT GTGACCCGAC TCTGGAGCGT CCCGTCGACC CTCCGCCTGA CCAGCTCCAC 
CTCATTCCAC TCTACAGCCA GGCCAAGACA CAAGGGGGAA GGTTACCAGG AGGATACAAT 
TACCTTCATG TGGCAAAACC AGTCGCCAGA AAAACTCCAT CAGCTCACAA AGGTCGTGAT 
CTGGTGACGG GCTGTGCTGA AACCGGGAAC TACTTTCACA TACGCCACAG TCAGTTTGAC 
GTGGTGGAGA AAATCACGTG TTTGTCCAAG ACTCCACTTC CTGTCTCCAA CCTCCAGAGT 
CTCTACGGCA AACACCAGCG GCTGCTCAAC AACATCTGCT CACGATACGA TGAGGGATTG 
ATCCAGGACA TGTACAGGCC TGATTACTAC CACAACTGTT CGCAACCAAG ACATCACTTA 
AATAGGACCT GCCTGACAAA AGGAAGTAGA CCAAAAGATA CTCATAATAT ATATATGCAA 
CTACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cfap61 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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