gaa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gaa
Entrez Gene ID	100002366
Full Name	glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II)
General protein information	Preferred Names glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) Names lysosomal alpha-glucosidase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299343.2	XP_009297618.1	lysosomal alpha-glucosidase isoform X2
XM_001921922.5	XP_001921957.2	lysosomal alpha-glucosidase isoform X1
XM_001921922.4	XP_001921957.2	lysosomal alpha-glucosidase isoform X1
XM_017354744.1	XP_017210233.1	lysosomal alpha-glucosidase isoform X3
XM_021473369.1	XP_021329044.1	lysosomal alpha-glucosidase isoform X2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC30D11.01c	NP_593216.2
Gallus gallus (chicken)	LOC422082	XP_420085.4
Canis lupus familiaris (dog)	GAA	XP_005624079.1
Mus musculus (house mouse)	Gaa	NP_032090.3
Schizosaccharomyces pombe (fission yeast)	agl1	NP_593996.1
Oryza sativa (rice)	Os01g0130400	NP_001041922.1
Pan troglodytes (chimpanzee)	GAA	XP_001160653.1
Rattus norvegicus (Norway rat)	Gaa	NP_954549.1
Danio rerio (zebrafish)	gaa	XP_001921957.2
Schizosaccharomyces pombe (fission yeast)	SPAC1039.11c	XP_001713119.1
Neurospora crassa	NCU09281	XP_958500.1
Bos taurus (cattle)	GAA	NP_776338.1
Xenopus tropicalis (tropical clawed frog)	gaa	XP_002932561.2
Magnaporthe oryzae (rice blast fungus)	MGG_10662	XP_003710096.1
Homo sapiens (human)	GAA	NP_001073272.1
Macaca mulatta (Rhesus monkey)	GAA	XP_001109980.1
Arabidopsis thaliana (thale cress)	XYL1	NP_177023.1
Arabidopsis thaliana (thale cress)	AT3G45940	NP_190180.1

Gene Ontology

The following gaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08170	XM_001921922.5 Latest version!	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa141736	XM_021473369.1 Latest version!	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODa47673	XM_009299343.2 Latest version!	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa58211	XM_017354744.1 Latest version!	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
ODa08170	XM_001921922.4	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gaa ( XM_001921922.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921957.2 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08170
Clone ID Related Accession (Same CDS sequence)	XM_001921922.5 , XM_001921922.4
Accession Version	XM_001921922.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921922.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT 
CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC 
GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG 
ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921957.2
CDS	259..3015
Translation	MAIIRCLNTFAVLTCVLLIIVYEDNTICVAFSPSKERQHSSNISYRIIKAQKYSHNVSTVPRDQCGIAVERRIDCARDRSLSRAECESRGCCYLPLPQSGLRGPPWCFYTVFYPGYKMGPMLPTERGHRAILTRSAPSYLPRDIHTLQLDVMAETQDRLHLTLKDPTSPRYEVPFVKSQSKGHKSMENPLYDVDFQPEPFGFSVRRKSNGRVLLNTTIGPLLFADQYLQLSTSLASSTVSGLGEHYTPITLDLDWSSVSLWNRDMAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQLGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNSTGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKVPVDGLWIDMNEPSNFVQGSVDGCPDSELEKPPYTPGVIGGQLNSGTLCVSAQQYLSNHYNLHNLYGLTEAIATHRALLKVKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYSKGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEESYVVSKPIKLNGSLDGLVLGEVRVFGVQTPPTAVWANGQKVPDFSYRLDTKVLTVAGLELPMTAKITIQWS

Target ORF information:

RefSeq Version	XM_001921922.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921922.5

ORF Insert Sequence:

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT 
CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC 
GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG 
ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gaa ( XM_001921922.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921957.2 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08170
Clone ID Related Accession (Same CDS sequence)	XM_001921922.5 , XM_001921922.4
Accession Version	XM_001921922.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal alpha-glucosidase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921922.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT 
CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC 
GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG 
ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921957.2
CDS	467..3223
Translation	MAIIRCLNTFAVLTCVLLIIVYEDNTICVAFSPSKERQHSSNISYRIIKAQKYSHNVSTVPRDQCGIAVERRIDCARDRSLSRAECESRGCCYLPLPQSGLRGPPWCFYTVFYPGYKMGPMLPTERGHRAILTRSAPSYLPRDIHTLQLDVMAETQDRLHLTLKDPTSPRYEVPFVKSQSKGHKSMENPLYDVDFQPEPFGFSVRRKSNGRVLLNTTIGPLLFADQYLQLSTSLASSTVSGLGEHYTPITLDLDWSSVSLWNRDMAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQLGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNSTGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKVPVDGLWIDMNEPSNFVQGSVDGCPDSELEKPPYTPGVIGGQLNSGTLCVSAQQYLSNHYNLHNLYGLTEAIATHRALLKVKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYSKGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEESYVVSKPIKLNGSLDGLVLGEVRVFGVQTPPTAVWANGQKVPDFSYRLDTKVLTVAGLELPMTAKITIQWS

Target ORF information:

RefSeq Version	XM_001921922.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921922.4

ORF Insert Sequence:

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GTCATATGTG GTCAGTAAAC CGATCAAATT AAACGGATCT 
CTTGATGGTT TGGTCCTGGG GGAAGTGCGT GTTTTCGGGG TCCAGACTCC ACCTACTGCC 
GTATGGGCCA ACGGACAGAA AGTTCCTGAC TTCTCATACA GATTAGATAC CAAGGTTCTG 
ACAGTAGCTG GTCTGGAACT ACCAATGACT GCCAAGATCA CAATACAGTG GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gaa ( XM_021473369.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329044.1 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa141736
Clone ID Related Accession (Same CDS sequence)	XM_021473369.1
Accession Version	XM_021473369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACCAC ATAGAAGTGC CAACCTGTAT GGCTCCCACC CTTTCTTCCT GGTCCAAGAA 
GGTGATGGAC AGGCACATGG AGTCTTCCTT CTCAACAGCA ATGCAATGGA AGTGTTTATG 
CAGCCTGCTC CAGCATTAAC ATGGGTGACC ATTGGAGGAA TTCTGGACTT CTTCATCTTC 
TTGGGCCCGA GTCCTCAGAG TGTGATTCAA CAGTATCAGG AGGTTATAGG ATATCCTATG 
ATGCCTCCCT ACTGGTCACT GGGTTTTCAT CTGTGTCGTT GGGGTTATAC CTCCACCAAC 
ATCACTAGGA CAGTAGTTCA GCTCATGCGG CAGGCCAAGA TACCTTTGGA TGTACAGTGG 
AATGACCTTG ATTATGCAGA CCAAAGAAGA GTCTTTACTT TCGACCCGCA GCGGTTTGGA 
GATTTGCCTC AAATGGTGGA AGATTTCCAT CAGCTGGGGA TGAAATATGT GCTCATTCTT 
GATCCAGGCA TCAGCAGCGC GAGTCCTCCT GGTTCATACA AACCGTTTGA CGATGGACTG 
AAGAAAGGAG TTTTCATCAA CAACTCCACT GGACAGATCC TTATTGGGAA GGTATGGCCG 
GGTCCGACTG CATTCCCAGA TTTCACCAAT CCTACAACAC AGGACTGGTG GATGGACTGG 
ATCAAAGACT TTTACAACAA GGTACCTGTG GATGGACTTT GGATAGACAT GAATGAGCCT 
TCAAATTTTG TCCAGGGCTC TGTGGATGGA TGTCCTGACT CTGAATTGGA GAAACCACCA 
TATACACCAG GTGTGATTGG AGGCCAGTTA AACTCAGGAA CTCTGTGTGT GTCCGCACAG 
CAGTATCTGT CCAATCATTA CAATCTACAC AACCTCTATG GGCTGACCGA AGCCATCGCC 
ACTCACAGGG CACTGCTAAA AGTGAAGAAA ACTCGGCCGT TTGTTTTATC TCGATCATCT 
TTTCCGGGAC TGGGTCGTTT CTCTGCACAC TGGACAGGAG ATGTGCGAAG TGACTGGGAA 
CAACTGCGCT TTTCAATACC CGCTGTGCTC CTGTTTGGAC TGTATGGCAT TCCATTAGTG 
GGAGCTGATG TTTGTGGATT TGGTGGAGAC ACTAACGAAG AGCTGTGTGT GCGCTGGACA 
CAACTTGGAG CCTTTTACCC CTTCATGAGA AACCACAATG ACAGACCTAA TGCTCCTCAG 
GAGCCATATG TATTTAGTCA GCGAGCCCAG GATGCCATGA GGATGGTGAT TAATCTTCGA 
TATTCTCTCC TGCCCTTCCT CTACACACTC TTCCATCACG CGCACACCTC CGCCAGCACC 
GTGGCCCGCC CTCTCTTCCT TCAGTTCCCC ACTGATCCAG ATTGCAGGAG CATTGACAGA 
CAGTTCCTGT GGGGGAGTTC ACTGCTCATC AGTCCAGTTC TGGAGCAGGG AGCAGTGGAG 
GTGATGGCGT ATCTCCCCCC GGGGACTTGG TACAGTCTGC ACAATGGCCA AACATATTAC 
AGTAAAGGAC AATACATTGT GTTTCCTGCT TCGTTGGACA CCATCATCGT TCACGTGAGA 
GAGAGGAGCA TCATCCCACA GCAGGCTCCT GCTCTGACCA CTGCAATATC TCGCAAAAAC 
CCTTTCACCC TAACGGTGGG ACTGTCAGTG GGAAATTTGG CCAAAGGAGA ACTTTTTTGG 
GATGATGGAG AAAGTCTGGA TACTTTTGAG CGGGGAGATT ATTCATATCT TCTCTTCTTT 
GCTGAAGAGT CATATGTGGT CAGTAAACCG ATCAAATTAA ACGGATCTCT TGATGGTTTG 
GTCCTGGGGG AAGTGCGTGT TTTCGGGGTC CAGACTCCAC CTACTGCCGT ATGGGCCAAC 
GGACAGAAAG TTCCTGACTT CTCATACAGA TTAGATACCA AGGTTCTGAC AGTAGCTGGT 
CTGGAACTAC CAATGACTGC CAAGATCACA ATACAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329044.1
CDS	219..2183
Translation	MAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQLGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNSTGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKVPVDGLWIDMNEPSNFVQGSVDGCPDSELEKPPYTPGVIGGQLNSGTLCVSAQQYLSNHYNLHNLYGLTEAIATHRALLKVKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYSKGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEESYVVSKPIKLNGSLDGLVLGEVRVFGVQTPPTAVWANGQKVPDFSYRLDTKVLTVAGLELPMTAKITIQWS

Target ORF information:

RefSeq Version	XM_021473369.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473369.1

ORF Insert Sequence:

ATGGCACCAC ATAGAAGTGC CAACCTGTAT GGCTCCCACC CTTTCTTCCT GGTCCAAGAA 
GGTGATGGAC AGGCACATGG AGTCTTCCTT CTCAACAGCA ATGCAATGGA AGTGTTTATG 
CAGCCTGCTC CAGCATTAAC ATGGGTGACC ATTGGAGGAA TTCTGGACTT CTTCATCTTC 
TTGGGCCCGA GTCCTCAGAG TGTGATTCAA CAGTATCAGG AGGTTATAGG ATATCCTATG 
ATGCCTCCCT ACTGGTCACT GGGTTTTCAT CTGTGTCGTT GGGGTTATAC CTCCACCAAC 
ATCACTAGGA CAGTAGTTCA GCTCATGCGG CAGGCCAAGA TACCTTTGGA TGTACAGTGG 
AATGACCTTG ATTATGCAGA CCAAAGAAGA GTCTTTACTT TCGACCCGCA GCGGTTTGGA 
GATTTGCCTC AAATGGTGGA AGATTTCCAT CAGCTGGGGA TGAAATATGT GCTCATTCTT 
GATCCAGGCA TCAGCAGCGC GAGTCCTCCT GGTTCATACA AACCGTTTGA CGATGGACTG 
AAGAAAGGAG TTTTCATCAA CAACTCCACT GGACAGATCC TTATTGGGAA GGTATGGCCG 
GGTCCGACTG CATTCCCAGA TTTCACCAAT CCTACAACAC AGGACTGGTG GATGGACTGG 
ATCAAAGACT TTTACAACAA GGTACCTGTG GATGGACTTT GGATAGACAT GAATGAGCCT 
TCAAATTTTG TCCAGGGCTC TGTGGATGGA TGTCCTGACT CTGAATTGGA GAAACCACCA 
TATACACCAG GTGTGATTGG AGGCCAGTTA AACTCAGGAA CTCTGTGTGT GTCCGCACAG 
CAGTATCTGT CCAATCATTA CAATCTACAC AACCTCTATG GGCTGACCGA AGCCATCGCC 
ACTCACAGGG CACTGCTAAA AGTGAAGAAA ACTCGGCCGT TTGTTTTATC TCGATCATCT 
TTTCCGGGAC TGGGTCGTTT CTCTGCACAC TGGACAGGAG ATGTGCGAAG TGACTGGGAA 
CAACTGCGCT TTTCAATACC CGCTGTGCTC CTGTTTGGAC TGTATGGCAT TCCATTAGTG 
GGAGCTGATG TTTGTGGATT TGGTGGAGAC ACTAACGAAG AGCTGTGTGT GCGCTGGACA 
CAACTTGGAG CCTTTTACCC CTTCATGAGA AACCACAATG ACAGACCTAA TGCTCCTCAG 
GAGCCATATG TATTTAGTCA GCGAGCCCAG GATGCCATGA GGATGGTGAT TAATCTTCGA 
TATTCTCTCC TGCCCTTCCT CTACACACTC TTCCATCACG CGCACACCTC CGCCAGCACC 
GTGGCCCGCC CTCTCTTCCT TCAGTTCCCC ACTGATCCAG ATTGCAGGAG CATTGACAGA 
CAGTTCCTGT GGGGGAGTTC ACTGCTCATC AGTCCAGTTC TGGAGCAGGG AGCAGTGGAG 
GTGATGGCGT ATCTCCCCCC GGGGACTTGG TACAGTCTGC ACAATGGCCA AACATATTAC 
AGTAAAGGAC AATACATTGT GTTTCCTGCT TCGTTGGACA CCATCATCGT TCACGTGAGA 
GAGAGGAGCA TCATCCCACA GCAGGCTCCT GCTCTGACCA CTGCAATATC TCGCAAAAAC 
CCTTTCACCC TAACGGTGGG ACTGTCAGTG GGAAATTTGG CCAAAGGAGA ACTTTTTTGG 
GATGATGGAG AAAGTCTGGA TACTTTTGAG CGGGGAGATT ATTCATATCT TCTCTTCTTT 
GCTGAAGAGT CATATGTGGT CAGTAAACCG ATCAAATTAA ACGGATCTCT TGATGGTTTG 
GTCCTGGGGG AAGTGCGTGT TTTCGGGGTC CAGACTCCAC CTACTGCCGT ATGGGCCAAC 
GGACAGAAAG TTCCTGACTT CTCATACAGA TTAGATACCA AGGTTCTGAC AGTAGCTGGT 
CTGGAACTAC CAATGACTGC CAAGATCACA ATACAGTGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gaa ( XM_009299343.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297618.1 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47673
Clone ID Related Accession (Same CDS sequence)	XM_009299343.2
Accession Version	XM_009299343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal alpha-glucosidase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGACATGA ATGAGCCTTC AAATTTTGTC 
CAGGGCTCTG TGGATGGATG TCCTGACTCT GAATTGGAGA AACCACCATA TACACCAGGT 
GTGATTGGAG GCCAGTTAAA CTCAGGAACT CTGTGTGTGT CCGCACAGCA GTATCTGTCC 
AATCATTACA ATCTACACAA CCTCTATGGG CTGACCGAAG CCATCGCCAC TCACAGGGCA 
CTGCTAAAAG TGAAGAAAAC TCGGCCGTTT GTTTTATCTC GATCATCTTT TCCGGGACTG 
GGTCGTTTCT CTGCACACTG GACAGGAGAT GTGCGAAGTG ACTGGGAACA ACTGCGCTTT 
TCAATACCCG CTGTGCTCCT GTTTGGACTG TATGGCATTC CATTAGTGGG AGCTGATGTT 
TGTGGATTTG GTGGAGACAC TAACGAAGAG CTGTGTGTGC GCTGGACACA ACTTGGAGCC 
TTTTACCCCT TCATGAGAAA CCACAATGAC AGACCTAATG CTCCTCAGGA GCCATATGTA 
TTTAGTCAGC GAGCCCAGGA TGCCATGAGG ATGGTGATTA ATCTTCGATA TTCTCTCCTG 
CCCTTCCTCT ACACACTCTT CCATCACGCG CACACCTCCG CCAGCACCGT GGCCCGCCCT 
CTCTTCCTTC AGTTCCCCAC TGATCCAGAT TGCAGGAGCA TTGACAGACA GTTCCTGTGG 
GGGAGTTCAC TGCTCATCAG TCCAGTTCTG GAGCAGGGAG CAGTGGAGGT GATGGCGTAT 
CTCCCCCCGG GGACTTGGTA CAGTCTGCAC AATGGCCAAA CATATTACAG TAAAGGACAA 
TACATTGTGT TTCCTGCTTC GTTGGACACC ATCATCGTTC ACGTGAGAGA GAGGAGCATC 
ATCCCACAGC AGGCTCCTGC TCTGACCACT GCAATATCTC GCAAAAACCC TTTCACCCTA 
ACGGTGGGAC TGTCAGTGGG AAATTTGGCC AAAGGAGAAC TTTTTTGGGA TGATGGAGAA 
AGTCTGGATA CTTTTGAGCG GGGAGATTAT TCATATCTTC TCTTCTTTGC TGAAGAGTCA 
TATGTGGTCA GTAAACCGAT CAAATTAAAC GGATCTCTTG ATGGTTTGGT CCTGGGGGAA 
GTGCGTGTTT TCGGGGTCCA GACTCCACCT ACTGCCGTAT GGGCCAACGG ACAGAAAGTT 
CCTGACTTCT CATACAGATT AGATACCAAG GTTCTGACAG TAGCTGGTCT GGAACTACCA 
ATGACTGCCA AGATCACAAT ACAGTGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297618.1
CDS	467..3199
Translation	MAIIRCLNTFAVLTCVLLIIVYEDNTICVAFSPSKERQHSSNISYRIIKAQKYSHNVSTVPRDQCGIAVERRIDCARDRSLSRAECESRGCCYLPLPQSGLRGPPWCFYTVFYPGYKMGPMLPTERGHRAILTRSAPSYLPRDIHTLQLDVMAETQDRLHLTLKDPTSPRYEVPFVKSQSKGHKSMENPLYDVDFQPEPFGFSVRRKSNGRVLLNTTIGPLLFADQYLQLSTSLASSTVSGLGEHYTPITLDLDWSSVSLWNRDMAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQLGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNSTGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKDMNEPSNFVQGSVDGCPDSELEKPPYTPGVIGGQLNSGTLCVSAQQYLSNHYNLHNLYGLTEAIATHRALLKVKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYSKGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEESYVVSKPIKLNGSLDGLVLGEVRVFGVQTPPTAVWANGQKVPDFSYRLDTKVLTVAGLELPMTAKITIQWS

Target ORF information:

RefSeq Version	XM_009299343.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299343.2

ORF Insert Sequence:

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGACATGA ATGAGCCTTC AAATTTTGTC 
CAGGGCTCTG TGGATGGATG TCCTGACTCT GAATTGGAGA AACCACCATA TACACCAGGT 
GTGATTGGAG GCCAGTTAAA CTCAGGAACT CTGTGTGTGT CCGCACAGCA GTATCTGTCC 
AATCATTACA ATCTACACAA CCTCTATGGG CTGACCGAAG CCATCGCCAC TCACAGGGCA 
CTGCTAAAAG TGAAGAAAAC TCGGCCGTTT GTTTTATCTC GATCATCTTT TCCGGGACTG 
GGTCGTTTCT CTGCACACTG GACAGGAGAT GTGCGAAGTG ACTGGGAACA ACTGCGCTTT 
TCAATACCCG CTGTGCTCCT GTTTGGACTG TATGGCATTC CATTAGTGGG AGCTGATGTT 
TGTGGATTTG GTGGAGACAC TAACGAAGAG CTGTGTGTGC GCTGGACACA ACTTGGAGCC 
TTTTACCCCT TCATGAGAAA CCACAATGAC AGACCTAATG CTCCTCAGGA GCCATATGTA 
TTTAGTCAGC GAGCCCAGGA TGCCATGAGG ATGGTGATTA ATCTTCGATA TTCTCTCCTG 
CCCTTCCTCT ACACACTCTT CCATCACGCG CACACCTCCG CCAGCACCGT GGCCCGCCCT 
CTCTTCCTTC AGTTCCCCAC TGATCCAGAT TGCAGGAGCA TTGACAGACA GTTCCTGTGG 
GGGAGTTCAC TGCTCATCAG TCCAGTTCTG GAGCAGGGAG CAGTGGAGGT GATGGCGTAT 
CTCCCCCCGG GGACTTGGTA CAGTCTGCAC AATGGCCAAA CATATTACAG TAAAGGACAA 
TACATTGTGT TTCCTGCTTC GTTGGACACC ATCATCGTTC ACGTGAGAGA GAGGAGCATC 
ATCCCACAGC AGGCTCCTGC TCTGACCACT GCAATATCTC GCAAAAACCC TTTCACCCTA 
ACGGTGGGAC TGTCAGTGGG AAATTTGGCC AAAGGAGAAC TTTTTTGGGA TGATGGAGAA 
AGTCTGGATA CTTTTGAGCG GGGAGATTAT TCATATCTTC TCTTCTTTGC TGAAGAGTCA 
TATGTGGTCA GTAAACCGAT CAAATTAAAC GGATCTCTTG ATGGTTTGGT CCTGGGGGAA 
GTGCGTGTTT TCGGGGTCCA GACTCCACCT ACTGCCGTAT GGGCCAACGG ACAGAAAGTT 
CCTGACTTCT CATACAGATT AGATACCAAG GTTCTGACAG TAGCTGGTCT GGAACTACCA 
ATGACTGCCA AGATCACAAT ACAGTGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gaa ( XM_017354744.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210233.1 Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

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CloneID	ODa58211
Clone ID Related Accession (Same CDS sequence)	XM_017354744.1
Accession Version	XM_017354744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	lysosomal alpha-glucosidase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GGTTCTGACA GTAGCTGGTC TGGAACTACC AATGACTGCC 
AAGATCACAA TACAGTGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210233.1
CDS	467..3070
Translation	MAIIRCLNTFAVLTCVLLIIVYEDNTICVAFSPSKERQHSSNISYRIIKAQKYSHNVSTVPRDQCGIAVERRIDCARDRSLSRAECESRGCCYLPLPQSGLRGPPWCFYTVFYPGYKMGPMLPTERGHRAILTRSAPSYLPRDIHTLQLDVMAETQDRLHLTLKDPTSPRYEVPFVKSQSKGHKSMENPLYDVDFQPEPFGFSVRRKSNGRVLLNTTIGPLLFADQYLQLSTSLASSTVSGLGEHYTPITLDLDWSSVSLWNRDMAPHRSANLYGSHPFFLVQEGDGQAHGVFLLNSNAMEVFMQPAPALTWVTIGGILDFFIFLGPSPQSVIQQYQEVIGYPMMPPYWSLGFHLCRWGYTSTNITRTVVQLMRQAKIPLDVQWNDLDYADQRRVFTFDPQRFGDLPQMVEDFHQLGMKYVLILDPGISSASPPGSYKPFDDGLKKGVFINNSTGQILIGKVWPGPTAFPDFTNPTTQDWWMDWIKDFYNKVPVDGLWIDMNEPSNFVQGSVDGCPDSELEKPPYTPGVIGGQLNSGTLCVSAQQYLSNHYNLHNLYGLTEAIATHRALLKVKKTRPFVLSRSSFPGLGRFSAHWTGDVRSDWEQLRFSIPAVLLFGLYGIPLVGADVCGFGGDTNEELCVRWTQLGAFYPFMRNHNDRPNAPQEPYVFSQRAQDAMRMVINLRYSLLPFLYTLFHHAHTSASTVARPLFLQFPTDPDCRSIDRQFLWGSSLLISPVLEQGAVEVMAYLPPGTWYSLHNGQTYYSKGQYIVFPASLDTIIVHVRERSIIPQQAPALTTAISRKNPFTLTVGLSVGNLAKGELFWDDGESLDTFERGDYSYLLFFAEEVLTVAGLELPMTAKITIQWS

Target ORF information:

RefSeq Version	XM_017354744.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) (gaa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354744.1

ORF Insert Sequence:

ATGGCCATAA TTAGATGTTT AAACACATTT GCAGTACTGA CTTGTGTACT TCTTATCATT 
GTGTATGAAG ACAACACTAT TTGTGTTGCA TTTTCACCAT CAAAAGAAAG GCAGCATAGT 
TCAAATATCT CATACAGGAT CATTAAAGCA CAGAAGTACA GTCATAATGT ATCAACTGTT 
CCTAGAGATC AGTGTGGCAT AGCTGTGGAG CGCCGCATTG ACTGTGCTAG GGACAGATCT 
TTGAGTCGGG CTGAATGTGA AAGCAGAGGA TGCTGTTATT TGCCCCTGCC TCAGTCTGGA 
TTAAGAGGAC CACCGTGGTG TTTCTACACA GTCTTCTACC CAGGATACAA AATGGGGCCC 
ATGTTGCCTA CAGAACGGGG CCACAGGGCC ATTCTCACTC GTTCAGCACC TTCATACCTG 
CCCCGGGACA TCCACACACT GCAGCTAGAT GTGATGGCTG AGACTCAGGA TCGTCTTCAT 
CTCACTTTGA AGGATCCTAC ATCTCCACGA TATGAGGTTC CCTTTGTGAA GTCCCAATCC 
AAAGGCCACA AGAGCATGGA AAATCCACTT TATGATGTTG ATTTCCAGCC AGAACCATTT 
GGGTTTAGTG TACGGCGAAA ATCCAATGGC CGTGTTCTTC TGAACACTAC AATAGGCCCT 
CTGCTGTTTG CAGATCAGTA TCTCCAGCTC TCTACCAGCC TGGCTTCCTC TACAGTCTCG 
GGACTGGGTG AACATTACAC TCCCATCACG CTGGACCTCG ACTGGAGCTC TGTTTCATTA 
TGGAACAGAG ACATGGCACC ACATAGAAGT GCCAACCTGT ATGGCTCCCA CCCTTTCTTC 
CTGGTCCAAG AAGGTGATGG ACAGGCACAT GGAGTCTTCC TTCTCAACAG CAATGCAATG 
GAAGTGTTTA TGCAGCCTGC TCCAGCATTA ACATGGGTGA CCATTGGAGG AATTCTGGAC 
TTCTTCATCT TCTTGGGCCC GAGTCCTCAG AGTGTGATTC AACAGTATCA GGAGGTTATA 
GGATATCCTA TGATGCCTCC CTACTGGTCA CTGGGTTTTC ATCTGTGTCG TTGGGGTTAT 
ACCTCCACCA ACATCACTAG GACAGTAGTT CAGCTCATGC GGCAGGCCAA GATACCTTTG 
GATGTACAGT GGAATGACCT TGATTATGCA GACCAAAGAA GAGTCTTTAC TTTCGACCCG 
CAGCGGTTTG GAGATTTGCC TCAAATGGTG GAAGATTTCC ATCAGCTGGG GATGAAATAT 
GTGCTCATTC TTGATCCAGG CATCAGCAGC GCGAGTCCTC CTGGTTCATA CAAACCGTTT 
GACGATGGAC TGAAGAAAGG AGTTTTCATC AACAACTCCA CTGGACAGAT CCTTATTGGG 
AAGGTATGGC CGGGTCCGAC TGCATTCCCA GATTTCACCA ATCCTACAAC ACAGGACTGG 
TGGATGGACT GGATCAAAGA CTTTTACAAC AAGGTACCTG TGGATGGACT TTGGATAGAC 
ATGAATGAGC CTTCAAATTT TGTCCAGGGC TCTGTGGATG GATGTCCTGA CTCTGAATTG 
GAGAAACCAC CATATACACC AGGTGTGATT GGAGGCCAGT TAAACTCAGG AACTCTGTGT 
GTGTCCGCAC AGCAGTATCT GTCCAATCAT TACAATCTAC ACAACCTCTA TGGGCTGACC 
GAAGCCATCG CCACTCACAG GGCACTGCTA AAAGTGAAGA AAACTCGGCC GTTTGTTTTA 
TCTCGATCAT CTTTTCCGGG ACTGGGTCGT TTCTCTGCAC ACTGGACAGG AGATGTGCGA 
AGTGACTGGG AACAACTGCG CTTTTCAATA CCCGCTGTGC TCCTGTTTGG ACTGTATGGC 
ATTCCATTAG TGGGAGCTGA TGTTTGTGGA TTTGGTGGAG ACACTAACGA AGAGCTGTGT 
GTGCGCTGGA CACAACTTGG AGCCTTTTAC CCCTTCATGA GAAACCACAA TGACAGACCT 
AATGCTCCTC AGGAGCCATA TGTATTTAGT CAGCGAGCCC AGGATGCCAT GAGGATGGTG 
ATTAATCTTC GATATTCTCT CCTGCCCTTC CTCTACACAC TCTTCCATCA CGCGCACACC 
TCCGCCAGCA CCGTGGCCCG CCCTCTCTTC CTTCAGTTCC CCACTGATCC AGATTGCAGG 
AGCATTGACA GACAGTTCCT GTGGGGGAGT TCACTGCTCA TCAGTCCAGT TCTGGAGCAG 
GGAGCAGTGG AGGTGATGGC GTATCTCCCC CCGGGGACTT GGTACAGTCT GCACAATGGC 
CAAACATATT ACAGTAAAGG ACAATACATT GTGTTTCCTG CTTCGTTGGA CACCATCATC 
GTTCACGTGA GAGAGAGGAG CATCATCCCA CAGCAGGCTC CTGCTCTGAC CACTGCAATA 
TCTCGCAAAA ACCCTTTCAC CCTAACGGTG GGACTGTCAG TGGGAAATTT GGCCAAAGGA 
GAACTTTTTT GGGATGATGG AGAAAGTCTG GATACTTTTG AGCGGGGAGA TTATTCATAT 
CTTCTCTTCT TTGCTGAAGA GGTTCTGACA GTAGCTGGTC TGGAACTACC AATGACTGCC 
AAGATCACAA TACAGTGGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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gaa cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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