nsd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nsd2
Entrez Gene ID	100000709
Full Name	nuclear receptor binding SET domain protein 2
Synonyms	fc12c04,si:rp71-77d7.2,whsc1,wu:fc12c04,wu:fi20c01
General protein information	Preferred Names nuclear receptor binding SET domain protein 2 Names histone-lysine N-methyltransferase NSD2 Wolf-Hirschhorn syndrome candidate 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082551.1	NP_001076020.1	histone-lysine N-methyltransferase NSD2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC712618	XP_002802212.1
Canis lupus familiaris (dog)	WHSC1	XP_536224.4
Mus musculus (house mouse)	Whsc1	NP_001074571.2
Bos taurus (cattle)	WHSC1	XP_005208360.1
Xenopus tropicalis (tropical clawed frog)	whsc1	XP_002936809.1
Homo sapiens (human)	WHSC1	NP_579877.1
Rattus norvegicus (Norway rat)	Whsc1	NP_001178481.1
Gallus gallus (chicken)	WHSC1	XP_420839.3
Danio rerio (zebrafish)	whsc1	NP_001076020.1
Pan troglodytes (chimpanzee)	WHSC1	XP_001146473.1

Gene Ontology
Bibliography

Related articles in PubMed

Wolf-Hirschhorn Syndrome Candidate 1 (whsc1) Functions as a Tumor Suppressor by Governing Cell Differentiation.
Yu C, Yao X, Zhao L, Wang P, Zhang Q, Zhao C, Yao S, Wei Y
Neoplasia (New York, N.Y.)19(8)606-616(2017 Aug)

Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.
Liu D, Wang Z, Xiao A, Zhang Y, Li W, Zu Y, Yao S, Lin S, Zhang B
Journal of genetics and genomics = Yi chuan xue bao41(1)43-6(2014 Jan)

A PCR based protocol for detecting indel mutations induced by TALENs and CRISPR/Cas9 in zebrafish.
Yu C, Zhang Y, Yao S, Wei Y
PloS one9(6)e98282(2014)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Functional characterization of the zebrafish WHSC1-related gene, a homolog of human NSD2.
Yamada-Okabe T, Imamura K, Kawaguchi N, Sakai H, Yamashita M, Matsumoto N
Biochemical and biophysical research communications402(2)335-9(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nsd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nsd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33897	NM_001082551.1 Latest version!	Danio rerio nuclear receptor binding SET domain protein 2 (nsd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nsd2 ( NM_001082551.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001076020.1 Danio rerio nuclear receptor binding SET domain protein 2 (nsd2), mRNA.

Price & Availability↑

CloneID	ODa33897
Clone ID Related Accession (Same CDS sequence)	NM_001082551.1
Accession Version	NM_001082551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529942400000
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase NSD2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR556722.7 and CT573111.9. On Mar 11, 2007 this sequence version replaced XM_001337457.1. ##Evidence-Data-START## Transcript exon combination :: BC171696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAGACA GTAAGGGGGG ATCGCTGCCT GCAATGCCAG AGCCCAAGAG TCCTACTAGC 
ATGAAACAGC TGTCGGATTC ACCCGGTGGA CGTAACGGTC TGGTTGATGG GATCACTGAT 
CCTACAATGC TCATGAATAA AGCTGCTACC CAGCTGGCAG CAACACCTCA AGAAAGTGTT 
CCGCAGATGA TTGGCAGTCA CGGTCATAAC CACACCCACG AGCATCTAAA GGACCTGACG 
TCCCGCTTGT TAAATGGCGA TACAGAAAAA ATTCCAAAGC TCTGTGCAGC CCCTGCCGGA 
CAGCCTCTTT TGAAAGGGGT TGAACCTGTT TCTCAACATG CCAGCCCTCC AAGGAAGACC 
AGCGTTTCTC CTGAACTTAC TGTCAAAATA ACCAAATGCG TGGTTAATTT AGGTGGGAAG 
CCAAACCGGT ATGAAGTAGA TTATACTGAG GAGAAAGCTG AGGAGACGGA TGCACCTTTG 
AGACTACCAG AAGGGGTTCT CACCTCTCCA ACTGCCAGGA GAGATAGACA AAAGAAGAAG 
GGTATGGTGA GGAAGAGGAT CCCACCTTAT CCAATCTTAA CCCATGCTGC AAGTTCTGAT 
CAATCTCCTC TACTGCACCC CACCATAAAT TCAAATGACA TCACTATTGA ACCGTGTGGT 
CTTCCTGTTG AGTCCATTGA GCACACTCAT GGAGACATGC CTACTTGTGT AACTGCCTTT 
CCAGATGAGC AACCGTTGTC TGACTGTGAA GAGACTGTTG ATGCACAGGG TGTGGATGAG 
ACTGATGATG CACAGGGTGA GAATGATGTA AAGGAGAGTT ATACCGAAAC TCAATCCAAT 
GTGCATTTCT CAGTGGGAGA TGTCATCTGG ACCAAAGTGT CTGGATATCC ATGGTGGCCG 
TGTATGATCA CTACAGACCC GGAGTTTAAC ATGCACTTTA GGTCAAAAGT GAATAGCCGT 
ACAGGATTAT TGTACCATGT ACAGTATTTT GGAGATACTC CAGAAAGAGG ATATGTTTTT 
GAGAAGTGCA TAGTCACCTT CTCAGGGAAA CACCAATACC AGTCACTTAG CCGCAGCAAC 
CAGCTCCCCA TTAATATCAG TGGAAGGAAG CGGGCAGTTC CTCGTAAGTT TCGGCAAAAG 
TGGGAAATAG GTGTGTCACA AGCAAAAGAA GCTTTGGGCC TGCTAACAGA GGAGCGGCTG 
GCCAAATTTA GCTTTGTTTA TGAAGATGGC AAAGCCCAGC TCAACCCTCA TATGCTTCAA 
GTGCTTGAGG AGCAACGCAA TCAGGGACTG CAGGAAGAGC CAGTTCAGAG AGAGCCCCAG 
GAGCTTGTCA GCCTCACGCC ACCTGACTCC ACTCATTCCA GCCCCACAAA CTCCTCAACA 
ACATCCACAA TCCCAAGCCT CTTCTCCAAC CCCATAGACA GCAGCACTTC ATCAGGGAAA 
ACCGCCCTCC CCAGGAAAGC ACGTAAACCG TCAGTGAAGA CGAATCGCCG CAAACAGCGC 
AGCTCGACAA TAGAGTGGAT AGTGACTGAA GCTTCCTCTG CAAAACAAAT GCCCCTGACT 
GAGTCTACTG TTCAGTGCGA GGTTAATACA GAGCACAAGA AGAAAGGAAA GACAAATGCT 
GGGAAACGGC CTTACCGGAA AAAAGATCTG CTCTGTATTG ATCTGGACCT TCAGCCAAAA 
GCTTCTCAGA AGAAACCAAG ACACATCAGG CAGAAGTTTC CTGCAGCACC AAATTCAGTG 
AAGAAAAGGA AAATTAAAGC AGGAGTCAGT ATCTGTGACA CGTTAAACAC AGTGTCTACT 
AGTGCAGGAC TTTCAGAAGT AAAGGCATCT GCACCAAGCA CAAAACTCTG GGAGAAGGAA 
GATGCCTCTT CTAAAGGAAT GAAAAAGGTC TGCAAGCGTT CACGAGAGTC AAAGCAGGAT 
GTGACATTGC AGCCTAAGAA GAAAAGCAAG CTTGAAAAAG ATTTTAAAAA ATGGAAATGC 
CCAAAGGCAG CATCATCTGC ACCGGCTGCT GTTCCTGCAC CCAGGGGGAG GAGGCGGAAA 
ACAGACAGAG ATGAAAATAG GAAACGATCT AAAAAAGCCT CAAAAGAGGA ACCTCCTCCT 
AAAGCCAAAC GTGGTCGCAA GCGGAAAGAG GAGTCGGGCA GGCAATCCTT TCCTGCCTCC 
AAAAAGTGCC GCCCATCATC TTACGCTGAG CCAGAGAGAC CCGATTCTCC CAGTGACAGT 
ACAGACGAAT CGCATCCCTC AAAAAAGGCC GAACGCACTC CAGGTGCCAA GAAAGAGAGC 
GTGTGTCTGG TGTGTGAGCA GACCGGGGAG GATATTGTGA CGTGTGCGGG ACAGTGCTAT 
GGCACATATC ATCTGCATTG TATTGGAGTA GAGCGGTCAG CAGAAAAGAT CCTGTGCACT 
GCCTGCAGTA CAGGTGTGCA TGTGTGTTTC ACCTGCAAGA AGTCTGAAGG AGAGGTGCGG 
CGCTGCTGTG CGCTCCACTG TGGCCGTTTC TATCATGAGG CCTGCGTGCG GTTGAGTGCT 
CTGACCGTGT TTGAAAACCG AGGCTTCCGC TGCCCGCTGC ACACCTGCCT CAGCTGCCAC 
TACAGTGGAC GCGCCGCCTG CAAGGCCACC AAAGGAAAGA TGATGCGATG TTTGCGGTGT 
CCTGTGGCCT ATCATGTGGG AGACCTGTGT GTGGCTGCAG GCAGTGAGAT GATCTCTTCC 
TCTGCCATTG TGTGCACAAA CCACTTCAGG GCTAAGAAAG GCTACAGTCA CCACTCGCAT 
GTCAACGTCA GCTGGTGCTT TATCTGCTCA AAAGGTGGCC GTCTGCTGTG TTGCGAGTCT 
TGTCCGGCTG CGTTTCACCC TGATTGTCTG AACATCGCTA TGCCTGATGG CAGCTGGTTT 
TGCAATGACT GCCGCTCAGG AAAGAAACCT AAATACAGGG ATGTCATCTG GGTCAAACTG 
GGGAACTACA GATGGTGGCC TGCAGAGATC CGTCATCCCA AGAACATCCC AACCAACATC 
CAGCACCTGC GTCATGAGAT TGGAGAGTTC CCCGTCTTCT TCTTTGGCTC TAAAGACTAT 
TTCTGGACGC ATCAGGGTCG AGTTTTTCCT TACATGGAGG GAGACAGGGG AAGCAAGTAC 
CAGCAGACTG GCATTGGGAA GGTCTTTAAG AATGCCCTGC TGGATGCTGA AACTCGCTTT 
AAGGAGATCG AGATGGAGAG AGAGGCAAAA GAGGCTCACG AAAATAACAA GAAGCCTCCT 
CCATTCAAAT ACATCAAGGT GAACAAGCCT TGTGGGCGAG TGCAGGTGTA CACCGCAGAC 
ATCTCTGAGA TCCCCAAGTG TAACTGTAAG CCGTCAACTG AGCGGCCCTG CAGCTTTGAG 
TCTGAGTGCC TGAACCGCAT GTTGCTGTAC GAGTGCCATC CTCAAGTGTG TCCGGCCGGA 
GAGCGATGCC AAAACCAGGA CTTCACTAAA CGCCTCTACC CCGAGACCAA GATCATCCGC 
ACTGCAGGCA AAGGCTGGGG TCTGATTTCG CTGCGGGACA TTAAGAAAGG CGAGTTTGTG 
AATGAATATG TGGGTGAGCT GATAGACGAG GAGGAGTGCA GAAGCAGAAT CAGACACGCT 
CAGGAGAATG ACATCACACA TTTCTATATG CTCACTATAG ACAAGGATCG GATTATAGAT 
GCGGGACCCA AAGGGAACTA CTCTCGCTTC ATGAACCACA GCTGCCAGCC CAATTGTGAG 
ACTCAAAAAT GGACTGTTAA TGGAGACACA CGCGTGGGCC TGTTTGCAGT GTGTGACATT 
CCTGCTGGCA CTGAGCTGAC GTTTAACTAT AACCTGGACT GTTTGGGAAA TGAGAAAACT 
GTGTGTCGCT GCGGAGCTCC CAACTGCAGT GGTTTCCTAG GAGACAGACC CAAGAATGGA 
CATACTTCTG AGCCTAAAGC CAAACTTCAG AAGAAAAAGC CCAAACGGAA AAGGGCCCGC 
AATGAAGGCA AGAAGTCTGA GGATGAGTGT TTCCGCTGTG GAGATGGAGG ACAGTTGGTT 
CTGTGTGATA AGAAGGGCTG CACCAAAGCC TATCACCTCT CCTGTCTGGA CCGCACCAAG 
AGACCTTTCG GCCGCTGGGA CTGTCCTTGG CATCACTGTG ACGTGTGCGG CAAGAACTCT 
GCTGCCTTCT GCCAGCTGTG CCCCAACTCA TTCTGCAAGG CTCACCAGGA GGGGGCGCTG 
CGCTCTCACC CACTCACAGG CCAGCTCTGC TGCCAGGAAC ATGAAGACTC CGACATCCGC 
CCGCAGACAG AGACCGCACA ACTACCACCA CCCACCAAAC CACGCAAATA CAGGCGCAGA 
GCGGAGGCCA AAACCACCAG CAAAGCACCT CTCAAGAAGA GGCCCAGAAA AAATGCAGAG 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076020.1
CDS	255..4640
Translation	MTDSKGGSLPAMPEPKSPTSMKQLSDSPGGRNGLVDGITDPTMLMNKAATQLAATPQESVPQMIGSHGHNHTHEHLKDLTSRLLNGDTEKIPKLCAAPAGQPLLKGVEPVSQHASPPRKTSVSPELTVKITKCVVNLGGKPNRYEVDYTEEKAEETDAPLRLPEGVLTSPTARRDRQKKKGMVRKRIPPYPILTHAASSDQSPLLHPTINSNDITIEPCGLPVESIEHTHGDMPTCVTAFPDEQPLSDCEETVDAQGVDETDDAQGENDVKESYTETQSNVHFSVGDVIWTKVSGYPWWPCMITTDPEFNMHFRSKVNSRTGLLYHVQYFGDTPERGYVFEKCIVTFSGKHQYQSLSRSNQLPINISGRKRAVPRKFRQKWEIGVSQAKEALGLLTEERLAKFSFVYEDGKAQLNPHMLQVLEEQRNQGLQEEPVQREPQELVSLTPPDSTHSSPTNSSTTSTIPSLFSNPIDSSTSSGKTALPRKARKPSVKTNRRKQRSSTIEWIVTEASSAKQMPLTESTVQCEVNTEHKKKGKTNAGKRPYRKKDLLCIDLDLQPKASQKKPRHIRQKFPAAPNSVKKRKIKAGVSICDTLNTVSTSAGLSEVKASAPSTKLWEKEDASSKGMKKVCKRSRESKQDVTLQPKKKSKLEKDFKKWKCPKAASSAPAAVPAPRGRRRKTDRDENRKRSKKASKEEPPPKAKRGRKRKEESGRQSFPASKKCRPSSYAEPERPDSPSDSTDESHPSKKAERTPGAKKESVCLVCEQTGEDIVTCAGQCYGTYHLHCIGVERSAEKILCTACSTGVHVCFTCKKSEGEVRRCCALHCGRFYHEACVRLSALTVFENRGFRCPLHTCLSCHYSGRAACKATKGKMMRCLRCPVAYHVGDLCVAAGSEMISSSAIVCTNHFRAKKGYSHHSHVNVSWCFICSKGGRLLCCESCPAAFHPDCLNIAMPDGSWFCNDCRSGKKPKYRDVIWVKLGNYRWWPAEIRHPKNIPTNIQHLRHEIGEFPVFFFGSKDYFWTHQGRVFPYMEGDRGSKYQQTGIGKVFKNALLDAETRFKEIEMEREAKEAHENNKKPPPFKYIKVNKPCGRVQVYTADISEIPKCNCKPSTERPCSFESECLNRMLLYECHPQVCPAGERCQNQDFTKRLYPETKIIRTAGKGWGLISLRDIKKGEFVNEYVGELIDEEECRSRIRHAQENDITHFYMLTIDKDRIIDAGPKGNYSRFMNHSCQPNCETQKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGAPNCSGFLGDRPKNGHTSEPKAKLQKKKPKRKRARNEGKKSEDECFRCGDGGQLVLCDKKGCTKAYHLSCLDRTKRPFGRWDCPWHHCDVCGKNSAAFCQLCPNSFCKAHQEGALRSHPLTGQLCCQEHEDSDIRPQTETAQLPPPTKPRKYRRRAEAKTTSKAPLKKRPRKNAEV

Target ORF information:

RefSeq Version	NM_001082551.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nuclear receptor binding SET domain protein 2 (nsd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082551.1

ORF Insert Sequence:

ATGACAGACA GTAAGGGGGG ATCGCTGCCT GCAATGCCAG AGCCCAAGAG TCCTACTAGC 
ATGAAACAGC TGTCGGATTC ACCCGGTGGA CGTAACGGTC TGGTTGATGG GATCACTGAT 
CCTACAATGC TCATGAATAA AGCTGCTACC CAGCTGGCAG CAACACCTCA AGAAAGTGTT 
CCGCAGATGA TTGGCAGTCA CGGTCATAAC CACACCCACG AGCATCTAAA GGACCTGACG 
TCCCGCTTGT TAAATGGCGA TACAGAAAAA ATTCCAAAGC TCTGTGCAGC CCCTGCCGGA 
CAGCCTCTTT TGAAAGGGGT TGAACCTGTT TCTCAACATG CCAGCCCTCC AAGGAAGACC 
AGCGTTTCTC CTGAACTTAC TGTCAAAATA ACCAAATGCG TGGTTAATTT AGGTGGGAAG 
CCAAACCGGT ATGAAGTAGA TTATACTGAG GAGAAAGCTG AGGAGACGGA TGCACCTTTG 
AGACTACCAG AAGGGGTTCT CACCTCTCCA ACTGCCAGGA GAGATAGACA AAAGAAGAAG 
GGTATGGTGA GGAAGAGGAT CCCACCTTAT CCAATCTTAA CCCATGCTGC AAGTTCTGAT 
CAATCTCCTC TACTGCACCC CACCATAAAT TCAAATGACA TCACTATTGA ACCGTGTGGT 
CTTCCTGTTG AGTCCATTGA GCACACTCAT GGAGACATGC CTACTTGTGT AACTGCCTTT 
CCAGATGAGC AACCGTTGTC TGACTGTGAA GAGACTGTTG ATGCACAGGG TGTGGATGAG 
ACTGATGATG CACAGGGTGA GAATGATGTA AAGGAGAGTT ATACCGAAAC TCAATCCAAT 
GTGCATTTCT CAGTGGGAGA TGTCATCTGG ACCAAAGTGT CTGGATATCC ATGGTGGCCG 
TGTATGATCA CTACAGACCC GGAGTTTAAC ATGCACTTTA GGTCAAAAGT GAATAGCCGT 
ACAGGATTAT TGTACCATGT ACAGTATTTT GGAGATACTC CAGAAAGAGG ATATGTTTTT 
GAGAAGTGCA TAGTCACCTT CTCAGGGAAA CACCAATACC AGTCACTTAG CCGCAGCAAC 
CAGCTCCCCA TTAATATCAG TGGAAGGAAG CGGGCAGTTC CTCGTAAGTT TCGGCAAAAG 
TGGGAAATAG GTGTGTCACA AGCAAAAGAA GCTTTGGGCC TGCTAACAGA GGAGCGGCTG 
GCCAAATTTA GCTTTGTTTA TGAAGATGGC AAAGCCCAGC TCAACCCTCA TATGCTTCAA 
GTGCTTGAGG AGCAACGCAA TCAGGGACTG CAGGAAGAGC CAGTTCAGAG AGAGCCCCAG 
GAGCTTGTCA GCCTCACGCC ACCTGACTCC ACTCATTCCA GCCCCACAAA CTCCTCAACA 
ACATCCACAA TCCCAAGCCT CTTCTCCAAC CCCATAGACA GCAGCACTTC ATCAGGGAAA 
ACCGCCCTCC CCAGGAAAGC ACGTAAACCG TCAGTGAAGA CGAATCGCCG CAAACAGCGC 
AGCTCGACAA TAGAGTGGAT AGTGACTGAA GCTTCCTCTG CAAAACAAAT GCCCCTGACT 
GAGTCTACTG TTCAGTGCGA GGTTAATACA GAGCACAAGA AGAAAGGAAA GACAAATGCT 
GGGAAACGGC CTTACCGGAA AAAAGATCTG CTCTGTATTG ATCTGGACCT TCAGCCAAAA 
GCTTCTCAGA AGAAACCAAG ACACATCAGG CAGAAGTTTC CTGCAGCACC AAATTCAGTG 
AAGAAAAGGA AAATTAAAGC AGGAGTCAGT ATCTGTGACA CGTTAAACAC AGTGTCTACT 
AGTGCAGGAC TTTCAGAAGT AAAGGCATCT GCACCAAGCA CAAAACTCTG GGAGAAGGAA 
GATGCCTCTT CTAAAGGAAT GAAAAAGGTC TGCAAGCGTT CACGAGAGTC AAAGCAGGAT 
GTGACATTGC AGCCTAAGAA GAAAAGCAAG CTTGAAAAAG ATTTTAAAAA ATGGAAATGC 
CCAAAGGCAG CATCATCTGC ACCGGCTGCT GTTCCTGCAC CCAGGGGGAG GAGGCGGAAA 
ACAGACAGAG ATGAAAATAG GAAACGATCT AAAAAAGCCT CAAAAGAGGA ACCTCCTCCT 
AAAGCCAAAC GTGGTCGCAA GCGGAAAGAG GAGTCGGGCA GGCAATCCTT TCCTGCCTCC 
AAAAAGTGCC GCCCATCATC TTACGCTGAG CCAGAGAGAC CCGATTCTCC CAGTGACAGT 
ACAGACGAAT CGCATCCCTC AAAAAAGGCC GAACGCACTC CAGGTGCCAA GAAAGAGAGC 
GTGTGTCTGG TGTGTGAGCA GACCGGGGAG GATATTGTGA CGTGTGCGGG ACAGTGCTAT 
GGCACATATC ATCTGCATTG TATTGGAGTA GAGCGGTCAG CAGAAAAGAT CCTGTGCACT 
GCCTGCAGTA CAGGTGTGCA TGTGTGTTTC ACCTGCAAGA AGTCTGAAGG AGAGGTGCGG 
CGCTGCTGTG CGCTCCACTG TGGCCGTTTC TATCATGAGG CCTGCGTGCG GTTGAGTGCT 
CTGACCGTGT TTGAAAACCG AGGCTTCCGC TGCCCGCTGC ACACCTGCCT CAGCTGCCAC 
TACAGTGGAC GCGCCGCCTG CAAGGCCACC AAAGGAAAGA TGATGCGATG TTTGCGGTGT 
CCTGTGGCCT ATCATGTGGG AGACCTGTGT GTGGCTGCAG GCAGTGAGAT GATCTCTTCC 
TCTGCCATTG TGTGCACAAA CCACTTCAGG GCTAAGAAAG GCTACAGTCA CCACTCGCAT 
GTCAACGTCA GCTGGTGCTT TATCTGCTCA AAAGGTGGCC GTCTGCTGTG TTGCGAGTCT 
TGTCCGGCTG CGTTTCACCC TGATTGTCTG AACATCGCTA TGCCTGATGG CAGCTGGTTT 
TGCAATGACT GCCGCTCAGG AAAGAAACCT AAATACAGGG ATGTCATCTG GGTCAAACTG 
GGGAACTACA GATGGTGGCC TGCAGAGATC CGTCATCCCA AGAACATCCC AACCAACATC 
CAGCACCTGC GTCATGAGAT TGGAGAGTTC CCCGTCTTCT TCTTTGGCTC TAAAGACTAT 
TTCTGGACGC ATCAGGGTCG AGTTTTTCCT TACATGGAGG GAGACAGGGG AAGCAAGTAC 
CAGCAGACTG GCATTGGGAA GGTCTTTAAG AATGCCCTGC TGGATGCTGA AACTCGCTTT 
AAGGAGATCG AGATGGAGAG AGAGGCAAAA GAGGCTCACG AAAATAACAA GAAGCCTCCT 
CCATTCAAAT ACATCAAGGT GAACAAGCCT TGTGGGCGAG TGCAGGTGTA CACCGCAGAC 
ATCTCTGAGA TCCCCAAGTG TAACTGTAAG CCGTCAACTG AGCGGCCCTG CAGCTTTGAG 
TCTGAGTGCC TGAACCGCAT GTTGCTGTAC GAGTGCCATC CTCAAGTGTG TCCGGCCGGA 
GAGCGATGCC AAAACCAGGA CTTCACTAAA CGCCTCTACC CCGAGACCAA GATCATCCGC 
ACTGCAGGCA AAGGCTGGGG TCTGATTTCG CTGCGGGACA TTAAGAAAGG CGAGTTTGTG 
AATGAATATG TGGGTGAGCT GATAGACGAG GAGGAGTGCA GAAGCAGAAT CAGACACGCT 
CAGGAGAATG ACATCACACA TTTCTATATG CTCACTATAG ACAAGGATCG GATTATAGAT 
GCGGGACCCA AAGGGAACTA CTCTCGCTTC ATGAACCACA GCTGCCAGCC CAATTGTGAG 
ACTCAAAAAT GGACTGTTAA TGGAGACACA CGCGTGGGCC TGTTTGCAGT GTGTGACATT 
CCTGCTGGCA CTGAGCTGAC GTTTAACTAT AACCTGGACT GTTTGGGAAA TGAGAAAACT 
GTGTGTCGCT GCGGAGCTCC CAACTGCAGT GGTTTCCTAG GAGACAGACC CAAGAATGGA 
CATACTTCTG AGCCTAAAGC CAAACTTCAG AAGAAAAAGC CCAAACGGAA AAGGGCCCGC 
AATGAAGGCA AGAAGTCTGA GGATGAGTGT TTCCGCTGTG GAGATGGAGG ACAGTTGGTT 
CTGTGTGATA AGAAGGGCTG CACCAAAGCC TATCACCTCT CCTGTCTGGA CCGCACCAAG 
AGACCTTTCG GCCGCTGGGA CTGTCCTTGG CATCACTGTG ACGTGTGCGG CAAGAACTCT 
GCTGCCTTCT GCCAGCTGTG CCCCAACTCA TTCTGCAAGG CTCACCAGGA GGGGGCGCTG 
CGCTCTCACC CACTCACAGG CCAGCTCTGC TGCCAGGAAC ATGAAGACTC CGACATCCGC 
CCGCAGACAG AGACCGCACA ACTACCACCA CCCACCAAAC CACGCAAATA CAGGCGCAGA 
GCGGAGGCCA AAACCACCAG CAAAGCACCT CTCAAGAAGA GGCCCAGAAA AAATGCAGAG 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wolf-Hirschhorn Syndrome Candidate 1 (whsc1) Functions as a Tumor Suppressor by Governing Cell Differentiation.
Neoplasia (New York, N.Y.)19(8)606-616(2017 Aug)
Yu C,Yao X,Zhao L,Wang P,Zhang Q,Zhao C,Yao S,Wei Y

Efficient gene targeting in zebrafish mediated by a zebrafish-codon-optimized cas9 and evaluation of off-targeting effect.
Journal of genetics and genomics = Yi chuan xue bao41(1)43-6(2014 Jan)
Liu D,Wang Z,Xiao A,Zhang Y,Li W,Zu Y,Yao S,Lin S,Zhang B

A PCR based protocol for detecting indel mutations induced by TALENs and CRISPR/Cas9 in zebrafish.
PloS one9(6)e98282(2014)
Yu C,Zhang Y,Yao S,Wei Y

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Functional characterization of the zebrafish WHSC1-related gene, a homolog of human NSD2.
Biochemical and biophysical research communications402(2)335-9(2010 Nov)
Yamada-Okabe T,Imamura K,Kawaguchi N,Sakai H,Yamashita M,Matsumoto N

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nsd2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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