CNJ00390 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNJ00390
Entrez Gene ID	3254112
Full Name	3-methyl-2-oxobutanoate hydroxymethyltransferase, putative
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

mRNA and Protein(s)

mRNA	Protein	Name
XM_567295.1	XP_567295.1	3-methyl-2-oxobutanoate hydroxymethyltransferase, putative
XM_567295.2	XP_567295.1	3-methyl-2-oxobutanoate hydroxymethyltransferase, putative

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Loftus BJ, Fung E, Roncaglia P, Rowley D, Amedeo P, Bruno D, Vamathevan J, Miranda M, Anderson IJ, Fraser JA, Allen JE, Bosdet IE, Brent MR, Chiu R, Doering TL, Donlin MJ, D'Souza CA, Fox DS, Grinberg V, Fu J, Fukushima M, Haas BJ, Huang JC, Janbon G, Jones SJ, Koo HL, Krzywinski MI, Kwon-Chung JK, Lengeler KB, Maiti R, Marra MA, Marra RE, Mathewson CA, Mitchell TG, Pertea M, Riggs FR, Salzberg SL, Schein JE, Shvartsbeyn A, Shin H, Shumway M, Specht CA, Suh BB, Tenney A, Utterback TR, Wickes BL, Wortman JR, Wye NH, Kronstad JW, Lodge JK, Heitman J, Davis RW, Fraser CM, Hyman RW
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNJ00390 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNJ00390 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCn00900	XM_567295.2 Latest version!	Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, putative (CNJ00390), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCn00900	XM_567295.1	Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNJ00390 ( XM_567295.1 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_567295.1 Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, partial mRNA.

Price & Availability↑

CloneID	OCn00900
Clone ID Related Accession (Same CDS sequence)	XM_567295.1 , XM_567295.2
Accession Version	XM_567295.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1345046400000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	3-methyl-2-oxobutanoate hydroxymethyltransferase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006679). The reference sequence was derived from mrna.CNJ00390.1. This record represents the complete annotated sequence of the C. neoformans JEC21 genome. A small number of gaps exist which are represented by NNNNs within the final sequence. The genome has been annotated by TIGR and the annotation data is available for viewing and download from the TIGR database (http://www.tigr.org/tdb/e2k1/cna1/). COMPLETENESS: incomplete on the 5' end.

ATGTTACCAA GAGCCCACAG ATTAGTCAAT CAAAGGCCAG GGATCGCTCT TCGACCTCAC 
GCAAGCCACG TTTCCTCAGC GGCAACTTCG GCAGTTAGAC GTTCGGTGGC AAGTCAAGCC 
CAAGTAGCCC AACCCCCGAT CCATCCTGAA ATTGATGGCA CATTCAATGA TCTGATCGGG 
GAAGGGACTA TGAACATGGG AATGAAACCT AGAGGAAGCG ACAGGACGTC ATTGCCATAC 
AGAAATATAA ACGAGATCGA ATTGGCGGAT CATCAGCCTG TCCGACATAG CGGAAGAAAG 
TGGTCATCTC TAGGCTTGCG AATAGAACAG AGTGATCTCG TCATCGATGA GTCGCCAGAA 
GTTTATAACT CTGCCTCGGA AGGGGAATAC CATGATTTTG GTCGAGAAGA AAGGAGGAGC 
CCAGCTGCTG TCTTGGGAAG CAAGCGTCTG GGAATGGTAG TCTTGCCGGA GGAAATGCAG 
CGAGGTATTC AAAGGCAAAT CGACCTCATG GACAATCCCC GAGATATTCG AAAATCATAT 
CTGGCGCTTC CCAATGTTCC TACACCCATT AGCGCTACAA AATCGGAGCG TAGAGATAAA 
CCGTTCCGCA CACCGGAAGG CGAGCTTGCC AAAGCATCCG CAATCCTTCC CGGAGAGTAT 
GGTGCGGTCA AGAATGTGCT GGAAGAATTG GAGAGAAGGT TAGGTCGAGA ATGGTTGACA 
AGTGCAAAGG AAGGAGAGAT CTTGGAGTTC TCATCTAGCC TGGGGTCTGG CCTTTGGGCT 
ATCATGGATG TCATGGGTGG TCTGCTCTCT TCTCGCCGGA GATGGCAGGA AGGACAGGAC 
AAGTTAAAAT ATCAATTTGT ACACTCTTCT AGACATGGTC TTGACCTCGT CCAAAGAATA 
GCAGAGGTCA TCCCTGAGGA ATCTGCCGAC GTTCAGTTCA ACCGCAGACA TGTCCATTCT 
TCGACCCCAT CACTAATTCT TTCCACATTC CATTTAACCT CTTTCCCTAC ATTACCAACC 
AGACAACTCT ACCTGCGTCA ACTTTTAGAG CTCTCATCTC CTTATATCGT CCTCATCGAA 
AGGTCAACAC CCCAAGGCTG GGCCGCTATC TCACAAGCCC GCTCATACTT ACTCGAGAAA 
TCAACTTCCG AAAACCCCCT TCACGTGGTA GCTCCCTGTC CTCACGATGG GAAGTGCCCT 
TTGGTCGGAA CCAAGGATGT TTGTGGATAT AGCCAGAGAC TTCAAAGGCC TTCTTTCCTG 
AGAAAAACAA AACACTCATC TAGGGGAGAG GAGGAAAAGG GTTACTGCTA CCTAGTGATC 
GCCAAGGGCG AGCGTCCCTC CGTTGGCACC GTAGCTGAGG ATATGAAAGT TGCTGGAAGG 
ATGGGAAAGG TGGGACGGGA AGCAGCTGAA AAAGCGTTGA TCAAGTCTCA AGGCCGATCG 
ATCATTCAAG AAGTTGAAGG TCACGAAGCG GTAATGGAAG TTGTGCGCCT GCATGAGATA 
GAGCCTGGTA TGGAGAACTA TTTTGAAGAG ACGTCACCCT CTGTCAACTC GGAAGAATTG 
GAAGAGAACT TAAGAAAGGA GGCGTATAGC TGGCCGAGAA TGGTTGCTCC ACCGATGAAG 
AGGAAGGGAC ATGTCACCAT GGATACTTGT TGCGCTGATG GTAACATACA ACGTCTTACC 
TATACCAAGT CGCATTCCAA GCAAAGCTAC CATGACGCTC GTAAATCTTC TTGGGGTGAT 
CTCTTCCCCC ACACTTCCAA AGGCAAGCCT GTTATTCGAA CCCGTGGTGT CAGACGACTT 
GCAAAGTCCG AAAACAATGG GGATGCGGAC GCAGTTATCA GCGAACTCTT ATCAGCAAGC 
TTGGAGGAAG AGATGGAACT CGAGAAAGCT ATGGAGGCGG TGGAGGTTGA CGAGCTGAAG 
GAATTGGAAA TGTTGGGCAT TAAAACTCCC AGGGCTGAAG TCTCCAAGGA AAGGCAGGAT 
GACGATATGC TGGTGTGGAA GTCGAACAAG AATGGACCTT TTGCTTCCGG GCAGAAAAGG 
AGCTACAGAA CTTTAACATC GACGAGGAGC ACTTCACAAC CTAAGACGCC ACCTTCCATT 
CAGACTCGAT CAATGTCAGC TCGCCCGGCT CCTCCAATTA GGCCCAAGGC GACCCTTTCA 
TCCTTGTTGT CACTCGCTAA ATCTGGCACC CCCATCAGCG TTCTCACTGC TTACGATTAC 
CCCACTGCTC TCCTCTCCGA ATCCTGCAAC CTCGATATGA CTCTCGTTGG AGATTCACTT 
TCTCAAGTCG CTCTCGGTCA TGAGACAACT ACAGCTATTA CCCTTGATGA GATGATCCAC 
CATGCACGAA CGGTTGTACG GGGTGCGAAA ACACCTTTTG TTTTTGCCGA TATGCCCTTT 
GGTAGCTTCG AGACTTCCTT AGAAGAAGGC GTCAGGAGTG TCTTGAGGAT GGTTAAAGAA 
GGTGGTGTAG ATGGTGTCAA AATCGAAGGT GGTCGCGAAA TTGTCCCTCT TGTTCGACGT 
CTTTCCGCTA TTGGTATCCC TGTTATGCCT CACCTCGGCC TGCAACCTCA ACGAGCCACC 
AGTCTGTCAG GCTACCTCGT TCAGGGGCGA ACCGCTCAAG CTGCTTATGA GATCCTGCAA 
AGTGCTCGAG AGCTCGCGAA TGCTGGGGCT TTCGCATTCC TTCTCGAGGC AATGCCCTCA 
AAGGTTGCGA AGCTCGTTAC TGAGGAAGTT GGCAAGAAGG GTGTATTCAC AATTGGTATT 
GGTGCTGGAA ATGGGACCAA CGGCCAGGTT CTGGTTATCA CCGACGTGCT TGGTATCTAC 
GCTGAGGATC CTCCTGAGGA TATAGCCACA CCCAACGTTA CAGGAGAAAC CGAGCTTGTC 
TCTACGTCTC GTGATTTCAC CAAGCCTCTC GACGCGCCAC GATTCGTTCG TCAGTTTGGG 
TCGCTCGGGC AAGAGATGCG CCGCGCTGTG CGTGCATATG TGCAAGCTGT TAAGGGAAGA 
AGCTTCCCCG ACTCCAAAGA GAGCTATGGG ATGAAGAAGG AGGAGTGGGA GACGTTTTTG 
GAGATGGTCA AGCGCGAGAA GGACCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_567295.1
CDS	1..3093
Translation	MLPRAHRLVNQRPGIALRPHASHVSSAATSAVRRSVASQAQVAQPPIHPEIDGTFNDLIGEGTMNMGMKPRGSDRTSLPYRNINEIELADHQPVRHSGRKWSSLGLRIEQSDLVIDESPEVYNSASEGEYHDFGREERRSPAAVLGSKRLGMVVLPEEMQRGIQRQIDLMDNPRDIRKSYLALPNVPTPISATKSERRDKPFRTPEGELAKASAILPGEYGAVKNVLEELERRLGREWLTSAKEGEILEFSSSLGSGLWAIMDVMGGLLSSRRRWQEGQDKLKYQFVHSSRHGLDLVQRIAEVIPEESADVQFNRRHVHSSTPSLILSTFHLTSFPTLPTRQLYLRQLLELSSPYIVLIERSTPQGWAAISQARSYLLEKSTSENPLHVVAPCPHDGKCPLVGTKDVCGYSQRLQRPSFLRKTKHSSRGEEEKGYCYLVIAKGERPSVGTVAEDMKVAGRMGKVGREAAEKALIKSQGRSIIQEVEGHEAVMEVVRLHEIEPGMENYFEETSPSVNSEELEENLRKEAYSWPRMVAPPMKRKGHVTMDTCCADGNIQRLTYTKSHSKQSYHDARKSSWGDLFPHTSKGKPVIRTRGVRRLAKSENNGDADAVISELLSASLEEEMELEKAMEAVEVDELKELEMLGIKTPRAEVSKERQDDDMLVWKSNKNGPFASGQKRSYRTLTSTRSTSQPKTPPSIQTRSMSARPAPPIRPKATLSSLLSLAKSGTPISVLTAYDYPTALLSESCNLDMTLVGDSLSQVALGHETTTAITLDEMIHHARTVVRGAKTPFVFADMPFGSFETSLEEGVRSVLRMVKEGGVDGVKIEGGREIVPLVRRLSAIGIPVMPHLGLQPQRATSLSGYLVQGRTAQAAYEILQSARELANAGAFAFLLEAMPSKVAKLVTEEVGKKGVFTIGIGAGNGTNGQVLVITDVLGIYAEDPPEDIATPNVTGETELVSTSRDFTKPLDAPRFVRQFGSLGQEMRRAVRAYVQAVKGRSFPDSKESYGMKKEEWETFLEMVKREKDQH

Target ORF information:

RefSeq Version	XM_567295.1
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_567295.1

ORF Insert Sequence:

ATGTTACCAA GAGCCCACAG ATTAGTCAAT CAAAGGCCAG GGATCGCTCT TCGACCTCAC 
GCAAGCCACG TTTCCTCAGC GGCAACTTCG GCAGTTAGAC GTTCGGTGGC AAGTCAAGCC 
CAAGTAGCCC AACCCCCGAT CCATCCTGAA ATTGATGGCA CATTCAATGA TCTGATCGGG 
GAAGGGACTA TGAACATGGG AATGAAACCT AGAGGAAGCG ACAGGACGTC ATTGCCATAC 
AGAAATATAA ACGAGATCGA ATTGGCGGAT CATCAGCCTG TCCGACATAG CGGAAGAAAG 
TGGTCATCTC TAGGCTTGCG AATAGAACAG AGTGATCTCG TCATCGATGA GTCGCCAGAA 
GTTTATAACT CTGCCTCGGA AGGGGAATAC CATGATTTTG GTCGAGAAGA AAGGAGGAGC 
CCAGCTGCTG TCTTGGGAAG CAAGCGTCTG GGAATGGTAG TCTTGCCGGA GGAAATGCAG 
CGAGGTATTC AAAGGCAAAT CGACCTCATG GACAATCCCC GAGATATTCG AAAATCATAT 
CTGGCGCTTC CCAATGTTCC TACACCCATT AGCGCTACAA AATCGGAGCG TAGAGATAAA 
CCGTTCCGCA CACCGGAAGG CGAGCTTGCC AAAGCATCCG CAATCCTTCC CGGAGAGTAT 
GGTGCGGTCA AGAATGTGCT GGAAGAATTG GAGAGAAGGT TAGGTCGAGA ATGGTTGACA 
AGTGCAAAGG AAGGAGAGAT CTTGGAGTTC TCATCTAGCC TGGGGTCTGG CCTTTGGGCT 
ATCATGGATG TCATGGGTGG TCTGCTCTCT TCTCGCCGGA GATGGCAGGA AGGACAGGAC 
AAGTTAAAAT ATCAATTTGT ACACTCTTCT AGACATGGTC TTGACCTCGT CCAAAGAATA 
GCAGAGGTCA TCCCTGAGGA ATCTGCCGAC GTTCAGTTCA ACCGCAGACA TGTCCATTCT 
TCGACCCCAT CACTAATTCT TTCCACATTC CATTTAACCT CTTTCCCTAC ATTACCAACC 
AGACAACTCT ACCTGCGTCA ACTTTTAGAG CTCTCATCTC CTTATATCGT CCTCATCGAA 
AGGTCAACAC CCCAAGGCTG GGCCGCTATC TCACAAGCCC GCTCATACTT ACTCGAGAAA 
TCAACTTCCG AAAACCCCCT TCACGTGGTA GCTCCCTGTC CTCACGATGG GAAGTGCCCT 
TTGGTCGGAA CCAAGGATGT TTGTGGATAT AGCCAGAGAC TTCAAAGGCC TTCTTTCCTG 
AGAAAAACAA AACACTCATC TAGGGGAGAG GAGGAAAAGG GTTACTGCTA CCTAGTGATC 
GCCAAGGGCG AGCGTCCCTC CGTTGGCACC GTAGCTGAGG ATATGAAAGT TGCTGGAAGG 
ATGGGAAAGG TGGGACGGGA AGCAGCTGAA AAAGCGTTGA TCAAGTCTCA AGGCCGATCG 
ATCATTCAAG AAGTTGAAGG TCACGAAGCG GTAATGGAAG TTGTGCGCCT GCATGAGATA 
GAGCCTGGTA TGGAGAACTA TTTTGAAGAG ACGTCACCCT CTGTCAACTC GGAAGAATTG 
GAAGAGAACT TAAGAAAGGA GGCGTATAGC TGGCCGAGAA TGGTTGCTCC ACCGATGAAG 
AGGAAGGGAC ATGTCACCAT GGATACTTGT TGCGCTGATG GTAACATACA ACGTCTTACC 
TATACCAAGT CGCATTCCAA GCAAAGCTAC CATGACGCTC GTAAATCTTC TTGGGGTGAT 
CTCTTCCCCC ACACTTCCAA AGGCAAGCCT GTTATTCGAA CCCGTGGTGT CAGACGACTT 
GCAAAGTCCG AAAACAATGG GGATGCGGAC GCAGTTATCA GCGAACTCTT ATCAGCAAGC 
TTGGAGGAAG AGATGGAACT CGAGAAAGCT ATGGAGGCGG TGGAGGTTGA CGAGCTGAAG 
GAATTGGAAA TGTTGGGCAT TAAAACTCCC AGGGCTGAAG TCTCCAAGGA AAGGCAGGAT 
GACGATATGC TGGTGTGGAA GTCGAACAAG AATGGACCTT TTGCTTCCGG GCAGAAAAGG 
AGCTACAGAA CTTTAACATC GACGAGGAGC ACTTCACAAC CTAAGACGCC ACCTTCCATT 
CAGACTCGAT CAATGTCAGC TCGCCCGGCT CCTCCAATTA GGCCCAAGGC GACCCTTTCA 
TCCTTGTTGT CACTCGCTAA ATCTGGCACC CCCATCAGCG TTCTCACTGC TTACGATTAC 
CCCACTGCTC TCCTCTCCGA ATCCTGCAAC CTCGATATGA CTCTCGTTGG AGATTCACTT 
TCTCAAGTCG CTCTCGGTCA TGAGACAACT ACAGCTATTA CCCTTGATGA GATGATCCAC 
CATGCACGAA CGGTTGTACG GGGTGCGAAA ACACCTTTTG TTTTTGCCGA TATGCCCTTT 
GGTAGCTTCG AGACTTCCTT AGAAGAAGGC GTCAGGAGTG TCTTGAGGAT GGTTAAAGAA 
GGTGGTGTAG ATGGTGTCAA AATCGAAGGT GGTCGCGAAA TTGTCCCTCT TGTTCGACGT 
CTTTCCGCTA TTGGTATCCC TGTTATGCCT CACCTCGGCC TGCAACCTCA ACGAGCCACC 
AGTCTGTCAG GCTACCTCGT TCAGGGGCGA ACCGCTCAAG CTGCTTATGA GATCCTGCAA 
AGTGCTCGAG AGCTCGCGAA TGCTGGGGCT TTCGCATTCC TTCTCGAGGC AATGCCCTCA 
AAGGTTGCGA AGCTCGTTAC TGAGGAAGTT GGCAAGAAGG GTGTATTCAC AATTGGTATT 
GGTGCTGGAA ATGGGACCAA CGGCCAGGTT CTGGTTATCA CCGACGTGCT TGGTATCTAC 
GCTGAGGATC CTCCTGAGGA TATAGCCACA CCCAACGTTA CAGGAGAAAC CGAGCTTGTC 
TCTACGTCTC GTGATTTCAC CAAGCCTCTC GACGCGCCAC GATTCGTTCG TCAGTTTGGG 
TCGCTCGGGC AAGAGATGCG CCGCGCTGTG CGTGCATATG TGCAAGCTGT TAAGGGAAGA 
AGCTTCCCCG ACTCCAAAGA GAGCTATGGG ATGAAGAAGG AGGAGTGGGA GACGTTTTTG 
GAGATGGTCA AGCGCGAGAA GGACCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNJ00390 ( XM_567295.2 ) cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21) -> XP_567295.1 Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, putative (CNJ00390), mRNA.

Price & Availability↑

CloneID	OCn00900
Clone ID Related Accession (Same CDS sequence)	XM_567295.1 , XM_567295.2
Accession Version	XM_567295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523289600000
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Product	3-methyl-2-oxobutanoate hydroxymethyltransferase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_006679). On Apr 11, 2018 this sequence version replaced XM_567295.1.

ATGTTACCAA GAGCCCACAG ATTAGTCAAT CAAAGGCCAG GGATCGCTCT TCGACCTCAC 
GCAAGCCACG TTTCCTCAGC GGCAACTTCG GCAGTTAGAC GTTCGGTGGC AAGTCAAGCC 
CAAGTAGCCC AACCCCCGAT CCATCCTGAA ATTGATGGCA CATTCAATGA TCTGATCGGG 
GAAGGGACTA TGAACATGGG AATGAAACCT AGAGGAAGCG ACAGGACGTC ATTGCCATAC 
AGAAATATAA ACGAGATCGA ATTGGCGGAT CATCAGCCTG TCCGACATAG CGGAAGAAAG 
TGGTCATCTC TAGGCTTGCG AATAGAACAG AGTGATCTCG TCATCGATGA GTCGCCAGAA 
GTTTATAACT CTGCCTCGGA AGGGGAATAC CATGATTTTG GTCGAGAAGA AAGGAGGAGC 
CCAGCTGCTG TCTTGGGAAG CAAGCGTCTG GGAATGGTAG TCTTGCCGGA GGAAATGCAG 
CGAGGTATTC AAAGGCAAAT CGACCTCATG GACAATCCCC GAGATATTCG AAAATCATAT 
CTGGCGCTTC CCAATGTTCC TACACCCATT AGCGCTACAA AATCGGAGCG TAGAGATAAA 
CCGTTCCGCA CACCGGAAGG CGAGCTTGCC AAAGCATCCG CAATCCTTCC CGGAGAGTAT 
GGTGCGGTCA AGAATGTGCT GGAAGAATTG GAGAGAAGGT TAGGTCGAGA ATGGTTGACA 
AGTGCAAAGG AAGGAGAGAT CTTGGAGTTC TCATCTAGCC TGGGGTCTGG CCTTTGGGCT 
ATCATGGATG TCATGGGTGG TCTGCTCTCT TCTCGCCGGA GATGGCAGGA AGGACAGGAC 
AAGTTAAAAT ATCAATTTGT ACACTCTTCT AGACATGGTC TTGACCTCGT CCAAAGAATA 
GCAGAGGTCA TCCCTGAGGA ATCTGCCGAC GTTCAGTTCA ACCGCAGACA TGTCCATTCT 
TCGACCCCAT CACTAATTCT TTCCACATTC CATTTAACCT CTTTCCCTAC ATTACCAACC 
AGACAACTCT ACCTGCGTCA ACTTTTAGAG CTCTCATCTC CTTATATCGT CCTCATCGAA 
AGGTCAACAC CCCAAGGCTG GGCCGCTATC TCACAAGCCC GCTCATACTT ACTCGAGAAA 
TCAACTTCCG AAAACCCCCT TCACGTGGTA GCTCCCTGTC CTCACGATGG GAAGTGCCCT 
TTGGTCGGAA CCAAGGATGT TTGTGGATAT AGCCAGAGAC TTCAAAGGCC TTCTTTCCTG 
AGAAAAACAA AACACTCATC TAGGGGAGAG GAGGAAAAGG GTTACTGCTA CCTAGTGATC 
GCCAAGGGCG AGCGTCCCTC CGTTGGCACC GTAGCTGAGG ATATGAAAGT TGCTGGAAGG 
ATGGGAAAGG TGGGACGGGA AGCAGCTGAA AAAGCGTTGA TCAAGTCTCA AGGCCGATCG 
ATCATTCAAG AAGTTGAAGG TCACGAAGCG GTAATGGAAG TTGTGCGCCT GCATGAGATA 
GAGCCTGGTA TGGAGAACTA TTTTGAAGAG ACGTCACCCT CTGTCAACTC GGAAGAATTG 
GAAGAGAACT TAAGAAAGGA GGCGTATAGC TGGCCGAGAA TGGTTGCTCC ACCGATGAAG 
AGGAAGGGAC ATGTCACCAT GGATACTTGT TGCGCTGATG GTAACATACA ACGTCTTACC 
TATACCAAGT CGCATTCCAA GCAAAGCTAC CATGACGCTC GTAAATCTTC TTGGGGTGAT 
CTCTTCCCCC ACACTTCCAA AGGCAAGCCT GTTATTCGAA CCCGTGGTGT CAGACGACTT 
GCAAAGTCCG AAAACAATGG GGATGCGGAC GCAGTTATCA GCGAACTCTT ATCAGCAAGC 
TTGGAGGAAG AGATGGAACT CGAGAAAGCT ATGGAGGCGG TGGAGGTTGA CGAGCTGAAG 
GAATTGGAAA TGTTGGGCAT TAAAACTCCC AGGGCTGAAG TCTCCAAGGA AAGGCAGGAT 
GACGATATGC TGGTGTGGAA GTCGAACAAG AATGGACCTT TTGCTTCCGG GCAGAAAAGG 
AGCTACAGAA CTTTAACATC GACGAGGAGC ACTTCACAAC CTAAGACGCC ACCTTCCATT 
CAGACTCGAT CAATGTCAGC TCGCCCGGCT CCTCCAATTA GGCCCAAGGC GACCCTTTCA 
TCCTTGTTGT CACTCGCTAA ATCTGGCACC CCCATCAGCG TTCTCACTGC TTACGATTAC 
CCCACTGCTC TCCTCTCCGA ATCCTGCAAC CTCGATATGA CTCTCGTTGG AGATTCACTT 
TCTCAAGTCG CTCTCGGTCA TGAGACAACT ACAGCTATTA CCCTTGATGA GATGATCCAC 
CATGCACGAA CGGTTGTACG GGGTGCGAAA ACACCTTTTG TTTTTGCCGA TATGCCCTTT 
GGTAGCTTCG AGACTTCCTT AGAAGAAGGC GTCAGGAGTG TCTTGAGGAT GGTTAAAGAA 
GGTGGTGTAG ATGGTGTCAA AATCGAAGGT GGTCGCGAAA TTGTCCCTCT TGTTCGACGT 
CTTTCCGCTA TTGGTATCCC TGTTATGCCT CACCTCGGCC TGCAACCTCA ACGAGCCACC 
AGTCTGTCAG GCTACCTCGT TCAGGGGCGA ACCGCTCAAG CTGCTTATGA GATCCTGCAA 
AGTGCTCGAG AGCTCGCGAA TGCTGGGGCT TTCGCATTCC TTCTCGAGGC AATGCCCTCA 
AAGGTTGCGA AGCTCGTTAC TGAGGAAGTT GGCAAGAAGG GTGTATTCAC AATTGGTATT 
GGTGCTGGAA ATGGGACCAA CGGCCAGGTT CTGGTTATCA CCGACGTGCT TGGTATCTAC 
GCTGAGGATC CTCCTGAGGA TATAGCCACA CCCAACGTTA CAGGAGAAAC CGAGCTTGTC 
TCTACGTCTC GTGATTTCAC CAAGCCTCTC GACGCGCCAC GATTCGTTCG TCAGTTTGGG 
TCGCTCGGGC AAGAGATGCG CCGCGCTGTG CGTGCATATG TGCAAGCTGT TAAGGGAAGA 
AGCTTCCCCG ACTCCAAAGA GAGCTATGGG ATGAAGAAGG AGGAGTGGGA GACGTTTTTG 
GAGATGGTCA AGCGCGAGAA GGACCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_567295.1
CDS	47..3139
Translation	MLPRAHRLVNQRPGIALRPHASHVSSAATSAVRRSVASQAQVAQPPIHPEIDGTFNDLIGEGTMNMGMKPRGSDRTSLPYRNINEIELADHQPVRHSGRKWSSLGLRIEQSDLVIDESPEVYNSASEGEYHDFGREERRSPAAVLGSKRLGMVVLPEEMQRGIQRQIDLMDNPRDIRKSYLALPNVPTPISATKSERRDKPFRTPEGELAKASAILPGEYGAVKNVLEELERRLGREWLTSAKEGEILEFSSSLGSGLWAIMDVMGGLLSSRRRWQEGQDKLKYQFVHSSRHGLDLVQRIAEVIPEESADVQFNRRHVHSSTPSLILSTFHLTSFPTLPTRQLYLRQLLELSSPYIVLIERSTPQGWAAISQARSYLLEKSTSENPLHVVAPCPHDGKCPLVGTKDVCGYSQRLQRPSFLRKTKHSSRGEEEKGYCYLVIAKGERPSVGTVAEDMKVAGRMGKVGREAAEKALIKSQGRSIIQEVEGHEAVMEVVRLHEIEPGMENYFEETSPSVNSEELEENLRKEAYSWPRMVAPPMKRKGHVTMDTCCADGNIQRLTYTKSHSKQSYHDARKSSWGDLFPHTSKGKPVIRTRGVRRLAKSENNGDADAVISELLSASLEEEMELEKAMEAVEVDELKELEMLGIKTPRAEVSKERQDDDMLVWKSNKNGPFASGQKRSYRTLTSTRSTSQPKTPPSIQTRSMSARPAPPIRPKATLSSLLSLAKSGTPISVLTAYDYPTALLSESCNLDMTLVGDSLSQVALGHETTTAITLDEMIHHARTVVRGAKTPFVFADMPFGSFETSLEEGVRSVLRMVKEGGVDGVKIEGGREIVPLVRRLSAIGIPVMPHLGLQPQRATSLSGYLVQGRTAQAAYEILQSARELANAGAFAFLLEAMPSKVAKLVTEEVGKKGVFTIGIGAGNGTNGQVLVITDVLGIYAEDPPEDIATPNVTGETELVSTSRDFTKPLDAPRFVRQFGSLGQEMRRAVRAYVQAVKGRSFPDSKESYGMKKEEWETFLEMVKREKDQH

Target ORF information:

RefSeq Version	XM_567295.2
Organism	Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)
Definition	Cryptococcus neoformans var. neoformans JEC21 3-methyl-2-oxobutanoate hydroxymethyltransferase, putative (CNJ00390), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_567295.2

ORF Insert Sequence:

ATGTTACCAA GAGCCCACAG ATTAGTCAAT CAAAGGCCAG GGATCGCTCT TCGACCTCAC 
GCAAGCCACG TTTCCTCAGC GGCAACTTCG GCAGTTAGAC GTTCGGTGGC AAGTCAAGCC 
CAAGTAGCCC AACCCCCGAT CCATCCTGAA ATTGATGGCA CATTCAATGA TCTGATCGGG 
GAAGGGACTA TGAACATGGG AATGAAACCT AGAGGAAGCG ACAGGACGTC ATTGCCATAC 
AGAAATATAA ACGAGATCGA ATTGGCGGAT CATCAGCCTG TCCGACATAG CGGAAGAAAG 
TGGTCATCTC TAGGCTTGCG AATAGAACAG AGTGATCTCG TCATCGATGA GTCGCCAGAA 
GTTTATAACT CTGCCTCGGA AGGGGAATAC CATGATTTTG GTCGAGAAGA AAGGAGGAGC 
CCAGCTGCTG TCTTGGGAAG CAAGCGTCTG GGAATGGTAG TCTTGCCGGA GGAAATGCAG 
CGAGGTATTC AAAGGCAAAT CGACCTCATG GACAATCCCC GAGATATTCG AAAATCATAT 
CTGGCGCTTC CCAATGTTCC TACACCCATT AGCGCTACAA AATCGGAGCG TAGAGATAAA 
CCGTTCCGCA CACCGGAAGG CGAGCTTGCC AAAGCATCCG CAATCCTTCC CGGAGAGTAT 
GGTGCGGTCA AGAATGTGCT GGAAGAATTG GAGAGAAGGT TAGGTCGAGA ATGGTTGACA 
AGTGCAAAGG AAGGAGAGAT CTTGGAGTTC TCATCTAGCC TGGGGTCTGG CCTTTGGGCT 
ATCATGGATG TCATGGGTGG TCTGCTCTCT TCTCGCCGGA GATGGCAGGA AGGACAGGAC 
AAGTTAAAAT ATCAATTTGT ACACTCTTCT AGACATGGTC TTGACCTCGT CCAAAGAATA 
GCAGAGGTCA TCCCTGAGGA ATCTGCCGAC GTTCAGTTCA ACCGCAGACA TGTCCATTCT 
TCGACCCCAT CACTAATTCT TTCCACATTC CATTTAACCT CTTTCCCTAC ATTACCAACC 
AGACAACTCT ACCTGCGTCA ACTTTTAGAG CTCTCATCTC CTTATATCGT CCTCATCGAA 
AGGTCAACAC CCCAAGGCTG GGCCGCTATC TCACAAGCCC GCTCATACTT ACTCGAGAAA 
TCAACTTCCG AAAACCCCCT TCACGTGGTA GCTCCCTGTC CTCACGATGG GAAGTGCCCT 
TTGGTCGGAA CCAAGGATGT TTGTGGATAT AGCCAGAGAC TTCAAAGGCC TTCTTTCCTG 
AGAAAAACAA AACACTCATC TAGGGGAGAG GAGGAAAAGG GTTACTGCTA CCTAGTGATC 
GCCAAGGGCG AGCGTCCCTC CGTTGGCACC GTAGCTGAGG ATATGAAAGT TGCTGGAAGG 
ATGGGAAAGG TGGGACGGGA AGCAGCTGAA AAAGCGTTGA TCAAGTCTCA AGGCCGATCG 
ATCATTCAAG AAGTTGAAGG TCACGAAGCG GTAATGGAAG TTGTGCGCCT GCATGAGATA 
GAGCCTGGTA TGGAGAACTA TTTTGAAGAG ACGTCACCCT CTGTCAACTC GGAAGAATTG 
GAAGAGAACT TAAGAAAGGA GGCGTATAGC TGGCCGAGAA TGGTTGCTCC ACCGATGAAG 
AGGAAGGGAC ATGTCACCAT GGATACTTGT TGCGCTGATG GTAACATACA ACGTCTTACC 
TATACCAAGT CGCATTCCAA GCAAAGCTAC CATGACGCTC GTAAATCTTC TTGGGGTGAT 
CTCTTCCCCC ACACTTCCAA AGGCAAGCCT GTTATTCGAA CCCGTGGTGT CAGACGACTT 
GCAAAGTCCG AAAACAATGG GGATGCGGAC GCAGTTATCA GCGAACTCTT ATCAGCAAGC 
TTGGAGGAAG AGATGGAACT CGAGAAAGCT ATGGAGGCGG TGGAGGTTGA CGAGCTGAAG 
GAATTGGAAA TGTTGGGCAT TAAAACTCCC AGGGCTGAAG TCTCCAAGGA AAGGCAGGAT 
GACGATATGC TGGTGTGGAA GTCGAACAAG AATGGACCTT TTGCTTCCGG GCAGAAAAGG 
AGCTACAGAA CTTTAACATC GACGAGGAGC ACTTCACAAC CTAAGACGCC ACCTTCCATT 
CAGACTCGAT CAATGTCAGC TCGCCCGGCT CCTCCAATTA GGCCCAAGGC GACCCTTTCA 
TCCTTGTTGT CACTCGCTAA ATCTGGCACC CCCATCAGCG TTCTCACTGC TTACGATTAC 
CCCACTGCTC TCCTCTCCGA ATCCTGCAAC CTCGATATGA CTCTCGTTGG AGATTCACTT 
TCTCAAGTCG CTCTCGGTCA TGAGACAACT ACAGCTATTA CCCTTGATGA GATGATCCAC 
CATGCACGAA CGGTTGTACG GGGTGCGAAA ACACCTTTTG TTTTTGCCGA TATGCCCTTT 
GGTAGCTTCG AGACTTCCTT AGAAGAAGGC GTCAGGAGTG TCTTGAGGAT GGTTAAAGAA 
GGTGGTGTAG ATGGTGTCAA AATCGAAGGT GGTCGCGAAA TTGTCCCTCT TGTTCGACGT 
CTTTCCGCTA TTGGTATCCC TGTTATGCCT CACCTCGGCC TGCAACCTCA ACGAGCCACC 
AGTCTGTCAG GCTACCTCGT TCAGGGGCGA ACCGCTCAAG CTGCTTATGA GATCCTGCAA 
AGTGCTCGAG AGCTCGCGAA TGCTGGGGCT TTCGCATTCC TTCTCGAGGC AATGCCCTCA 
AAGGTTGCGA AGCTCGTTAC TGAGGAAGTT GGCAAGAAGG GTGTATTCAC AATTGGTATT 
GGTGCTGGAA ATGGGACCAA CGGCCAGGTT CTGGTTATCA CCGACGTGCT TGGTATCTAC 
GCTGAGGATC CTCCTGAGGA TATAGCCACA CCCAACGTTA CAGGAGAAAC CGAGCTTGTC 
TCTACGTCTC GTGATTTCAC CAAGCCTCTC GACGCGCCAC GATTCGTTCG TCAGTTTGGG 
TCGCTCGGGC AAGAGATGCG CCGCGCTGTG CGTGCATATG TGCAAGCTGT TAAGGGAAGA 
AGCTTCCCCG ACTCCAAAGA GAGCTATGGG ATGAAGAAGG AGGAGTGGGA GACGTTTTTG 
GAGATGGTCA AGCGCGAGAA GGACCAGCAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans.
Science (New York, N.Y.)307(5713)1321-4(2005 Feb)
Loftus BJ,Fung E,Roncaglia P,Rowley D,Amedeo P,Bruno D,Vamathevan J,Miranda M,Anderson IJ,Fraser JA,Allen JE,Bosdet IE,Brent MR,Chiu R,Doering TL,Donlin MJ,D'Souza CA,Fox DS,Grinberg V,Fu J,Fukushima M,Haas BJ,Huang JC,Janbon G,Jones SJ,Koo HL,Krzywinski MI,Kwon-Chung JK,Lengeler KB,Maiti R,Marra MA,Marra RE,Mathewson CA,Mitchell TG,Pertea M,Riggs FR,Salzberg SL,Schein JE,Shvartsbeyn A,Shin H,Shumway M,Specht CA,Suh BB,Tenney A,Utterback TR,Wickes BL,Wortman JR,Wye NH,Kronstad JW,Lodge JK,Heitman J,Davis RW,Fraser CM,Hyman RW

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CNJ00390 cDNA ORF clone, Cryptococcus neoformans var. neoformans JEC21(Filobasidiella neoformans var. neoformans strain JEC21)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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