NOTCH1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOTCH1
Entrez Gene ID	103239621
Full Name	notch 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008005608.1	XP_008003799.1	neurogenic locus notch homolog protein 1 isoform X1
XM_008005609.1	XP_008003800.1	neurogenic locus notch homolog protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following NOTCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh98790	XM_008005608.1 Latest version!	Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh98791	XM_008005609.1 Latest version!	Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOTCH1 ( XM_008005608.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008003799.1 Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X1, mRNA.

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CloneID	OCh98790
Clone ID Related Accession (Same CDS sequence)	XM_008005608.1
Accession Version	XM_008005608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	neurogenic locus notch homolog protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGTTTT TCCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC GGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTTGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCAACCC CTGCAAGAAC ACCGGGACAT GCCACGTGGT GGACCGTGGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTCCC CTGGGCTTCT CTGGGCCCCT CTGCCTCACG 
CCCCTGGACA ACGCTTGCCT CACCAACCCC TGCCGCAATG GGGGCACCTG CGACCTGCTC 
ACGCTCACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCCT GCGCCTCCAA CCCCTGTGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CACGGTCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGCGGCC AGAACCCGGG GCTCTGCCGC CATGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACCGGGCCCA ACTGTGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG TGACGTCACC 
CACGAGTGCA CCTGCCTGCC AGGCTTCACC GGCCAGAACT GCGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAATGGGGGC GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGACGTGGA TGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACCGGTGAG GACTGCAGCG AGAACATCGA TGACTGCGCC 
AGCGCCGCCT GCTTCCACGG TGCCACCTGC CACGACCGCG TGGCCTCCTT CTACTGCGAG 
TGTCCCCACG GCCGCACAGG TCTGCTGTGT CACCTCAACG ATGCGTGTAT CAGTAATCCC 
TGCAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAACG GCAAGGCCAT CTGCACGTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGATGTGG ACGAGTGCTC GCTGGGTGCC 
AACCCCTGTG AGCATGCGGG CAAGTGCATC AACACACTGG GCTCCTTCGA GTGCCAGTGC 
CTGCAGGGCT ACACGGGCCC CCGCTGCGAG ATCGACGTCA ACGAGTGTGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTATGAGG GTGTGCACTG CGAGGTCAAC ACGGATGAGT GCGCCAGCAG CCCCTGCCTG 
CACAATGGCC ACTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGACACC TCTGCCAGTA TGACGTGGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGCGTGT GCACAGAAGG GTATACGGGG 
ATGCACTGCG AGGTGGACAT TGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCGG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAATG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGTCTC AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGACG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATTGAC 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGCAACGT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCC AGGACGGCAT CAACGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGTGTCCA CGGGGCGTGC CGGGACAGCC TCAATGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGCGG GACCAACTGT GACATCAACA ACAATGAGTG TGAGTCCAAC 
CCTTGTGTCA ACGGTGGTAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GTGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCATC CAACCCGTGT 
CTGAACCAGG GCACGTGTAT TGACGATGTT GCGGGGTACA AGTGCAACTG CCTGCTTCCC 
TACACAGGTG CCACGTGCGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGTGGGGAGT GCAGGGAATC TGAGGACTAT GAGAGCTTCT CCTGCGTCTG CCCCACAGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AATGAGTGTG TTATGAGCCC GTGCCGGCAC 
GGCGCGTCCT GCCGGAACAC CCATGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGC 
GGGCGCAACT GCGAGACGGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGCGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGATTGCC TGCCCGGCTT CCAGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAATGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGTCCCGCGG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCCGACTG CACGGAGAGC TCCTGCTTCA ATGGTGGCAC CTGCGTGGAC 
GGAATCAACT CGTTCACCTG CCTGTGTCCA CCTGGCTTCA CGGGCAGCTA TTGCCAGCAC 
GATGTCAACG AGTGCGACTC ACAGCCCTGC CTGCACGGCG GCACCTGTCA GGATGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACCGGCC CCAACTGCCA GAACCTCGTG 
CACTGGTGTG ACTCCTCACC CTGCAAGAAT GGTGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG TGGCTGGACC GGCCTTTACT GTGATGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGCGTT GATGTTGCCC GGCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ATGCGGGCAA CACACACCAC TGCCGTTGCC AGGCAGGCTA CACGGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTTA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACAGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TTGACGAGTG CCTGTCACAC CCCTGCCAGA ATGGGGGCAC CTGCCTTGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACAC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAACCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTCAAC 
AATGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGTA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GCGAGGGGGA TGTCAACGAG TGCCTCTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAACGAC TTCCACTGCG AGTGCCGTGC CGGTCACACC 
GGGCGCCGCT GCGAGTCAGT CATCAACGGC TGCAAGGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGTGCCG TGGCCTCCAA CACCGCCCGT GGATTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGCGAGAA TGACGCTCGC ACCTGCGGCA GCCTGCGCTG CCTCAACGGT 
GGCACGTGCA TCTCTGGCCC ACGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCTGAGTGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACGTC GGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GCGGGGCCGG GCGCGACATC 
CCCCCGCCGC AGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG CGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCCC TGCAGTGCTG GAAGTACTTC 
AGCGACGGCC ACTGTGACAG CCAGTGCAAC TCGGCCGGCT GCCTCTTCGA TGGCTTCGAC 
TGCCAGCGCG CGGAAGGCCA GTGCAACCCC CTGTATGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGCGCGGAGC ATGTGCCGGA GAGGCTGGCG GCGGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGGGTGCTG 
CACACCAACG TGGTCTTCAA GCGCGACGCG CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCACGAGG AAGAGCTTCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCACC 
CCCGAGGCCT TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG TGGTGGGCGG 
CGGCGGAGGG AGCTGGACCC CATGGACGTC CGCGGCTCCA TCGTCTACCT GGAGATTGAC 
AACCGGCAGT GTGTGCAGGC CTCCTCGCAG TGCTTCCAGA GCGCCACCGA CGTGGCCGCG 
TTCCTGGGGG CGCTCGCCTC GCTGGGCAGC CTCAACATCC CCTACAAGAT CGAGGCCGTG 
CAGAGTGAGA CCGTGGAGCC GCCCCCGCCA GCGCAGCTGC ACTTCATGTA CGTGGCGGCG 
GCCGCCTTTG TGCTCCTGTT CTTCGTGGGC TGCGGGGTGC TGCTGTCCCG CAAGCGCCGG 
CGGCAGCATG GCCAACTCTG GTTCCCTGAG GGCTTCAAGG TGTCCGAGGC CAGCAAGAAG 
AAGCGGCGGG AGCCCCTCGG CGAGGACTCT GTGGGCCTCA AGCCCCTGAA GAATGCCTCA 
GATGGTGCCC TCATGGACGA CAACCAGAAT GAGTGGGGGG ATGAGGACCT GGAGACCAAG 
AAGTTCCGGT TCGAGGAGCC CGTGGTTCTG CCTGACCTGG ACGACCAGAC AGACCACCGG 
CAGTGGACTC AGCAGCACCT GGATGCCGCT GACCTGCGCA TGTCTGCCAT GGCCCCCACG 
CCGCCCCAGG GTGAGGTTGA CGCCGACTGC ATGGACGTCA ATGTCCGCGG GCCTGACGGC 
TTCACCCCCC TCATGATCGC CTCCTGCAGT GGGGGCGGCC TGGAGACGGG CAACAGCGAG 
GAGGAGGAGG ACGCGCCGGC CGTCATCTCC GACTTCATCT ACCAGGGCGC CAGCCTGCAC 
AACCAGACCG ACCGCACGGG CGAGACCGCC TTGCACCTGG CTGCCCGCTA CTCACGCTCT 
GATGCCGCCA AGCGCCTGCT GGAGGCCAGT GCAGACGCCA ACATCCAGGA CAACATGGGC 
CGCACCCCGC TGCACGCAGC TGTGTCTGCC GACGCACAAG GTGTCTTCCA GATCCTGATC 
CGGAACCGAG CCACGGACCT GGATGCCCGC ATGCACGACG GCACGACGCC GCTGATCCTG 
GCTGCCCGCC TGGCTGTGGA GGGCATGCTG GAGGACCTCA TCAACTCTCA CGCCGATGTC 
AATGCTGTGG ACGACTTGGG CAAGTCCGCC CTGCACTGGG CCGCCGCCGT GAACAACGTG 
GACGCCGCGG TCGTGCTCCT GAAGAACGGG GCCAATAAAG ACATGCAGAA CAACAAGGAG 
GAGACACCCC TGTTTCTGGC CGCCCGGGAG GGGAGCTACG AGACCGCCAA GGTGCTGCTG 
GACCACTTTG CCAACCGGGA CATCACGGAC CATATGGATC GCCTGCCACG TGACATCGCA 
CAGGAGCGCA TGCACCACGA CATCGTGAGG CTGCTGGACG AGTACAACCT GGTGCGCAGC 
CCACAGCTGC ACGGAGCCAC ACTGGGGGGC ACGCCCACCC TGTCACCCCC GCTCTGCTCA 
CCCAACGGCT ACCTGGGCAG CCTCAAGCCC GGCGTGCAGG GTAAGAAGGT CCGCAAGCCC 
AGCAGCAAAG GCCTGGCCTG TGGGAGCAAG GAGGCCAAGG ACCTCAAGGC GCGGAGGAAG 
AAGTCCCAGG ACGGCAAGGG CTGCCTGCTG GACAGCTCCG GCATGCTCTC ACCCGTGGAC 
TCCCTGGAGT CGCCCCACGG CTACCTGTCG GACGTGGCCT CGCCGCCACT GCTGCCCTCT 
CCGTTCCAGC AGTCTCCGTC CGTGCCCCTC AACCACCTGC CCGGTATGCC CGACACCCAC 
CTGGGCATCG GGCACCTGAA CGTGGCGGCC AAGCCAGAGA TGGCAACACT GGGTGGGGGC 
GGCCGGCTGG CCTTCGAGGC CGGCCCCCCG CGTCTCTCCC ACCTGCCCGT GGCCTCCAGC 
ACCAGCACCA TCCTGGGCTG CAGCAGTGGA GGAGCCATGA ATTTCACCGT GGGTGGGTCC 
ACCAGTTTGA ATGGTCAATG CGAGTGGCTG TCCCGGCTGC AGAGCGGCAT GGTGCCGAAC 
CAATACAACC CTCTGCGGGG GAGCGTGGCA CCAGGCCCCC TAAGCACGCA GGCCCCCTCC 
CTGCAGCATG GCATGGTGGG CCCGCTGCAC AGTAGCCTTG CTGCCAGCGC CCTGTCCCAG 
ATGATGAGCT ACCAGGGCCT GCCCAGTACC CGGCTGGCCA CCCAGCCTCA CCTGGTGCAG 
ACCCAGCAGG TGCAGCCGCA AAACTTACAG ATGCAGCAGC AGAACCTGCA GCCACCAAAC 
ATCCAGCAGC AGCAAAATCT GCAGCCGCCA CCGCCACCAC CACAGCCGCA CCTTGGCGTG 
AGCTCAGCAG CCAGCGGCCA CCTGGGCCGG AGCTTCCTGG GCGGAGAGCC GAGCCAGGCA 
GACGTGCAGC CACTAGGCCC CAGCAGCCTG GCGGTGCACA CCATTCTACC CCAGGAGAGC 
CCTGCTCTGC CCACGTCGCT GCCGTCCTCG CTGGTCCCAC CCGTGACCGC GGCCCAGTTC 
CTGACACCCC CCTCACAGCA CAGCTACTCC TCCTCGCCTG TGGACAACAC ACCCAGCCAC 
CAACTGCAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CCGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCTG AGGGCGTCTC CAGCCCGCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008003799.1
CDS	256..7923
Translation	MPPLLAPLLCLAFFPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSNPCKNTGTCHVVDRGGVADYACSCPLGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQNPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECTCLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGHCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGMHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNVNIDECAGNPCHNGGTCQDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRESEDYESFSCVCPTGWQGQTCEVDINECVMSPCRHGASCRNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFQGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECLPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPQIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGHEEELRKHPIKRAAEGWATPEALLGQVKASLLPGGSGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGATLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMATLGGGGRLAFEAGPPRLSHLPVASSTSTILGCSSGGAMNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPPNIQQQQNLQPPPPPPQPHLGVSSAASGHLGRSFLGGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	XM_008005608.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005608.1

ORF Insert Sequence:

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGTTTT TCCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC GGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTTGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCAACCC CTGCAAGAAC ACCGGGACAT GCCACGTGGT GGACCGTGGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTCCC CTGGGCTTCT CTGGGCCCCT CTGCCTCACG 
CCCCTGGACA ACGCTTGCCT CACCAACCCC TGCCGCAATG GGGGCACCTG CGACCTGCTC 
ACGCTCACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCCT GCGCCTCCAA CCCCTGTGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CACGGTCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGCGGCC AGAACCCGGG GCTCTGCCGC CATGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACCGGGCCCA ACTGTGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG TGACGTCACC 
CACGAGTGCA CCTGCCTGCC AGGCTTCACC GGCCAGAACT GCGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAATGGGGGC GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGACGTGGA TGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACCGGTGAG GACTGCAGCG AGAACATCGA TGACTGCGCC 
AGCGCCGCCT GCTTCCACGG TGCCACCTGC CACGACCGCG TGGCCTCCTT CTACTGCGAG 
TGTCCCCACG GCCGCACAGG TCTGCTGTGT CACCTCAACG ATGCGTGTAT CAGTAATCCC 
TGCAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAACG GCAAGGCCAT CTGCACGTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGATGTGG ACGAGTGCTC GCTGGGTGCC 
AACCCCTGTG AGCATGCGGG CAAGTGCATC AACACACTGG GCTCCTTCGA GTGCCAGTGC 
CTGCAGGGCT ACACGGGCCC CCGCTGCGAG ATCGACGTCA ACGAGTGTGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTATGAGG GTGTGCACTG CGAGGTCAAC ACGGATGAGT GCGCCAGCAG CCCCTGCCTG 
CACAATGGCC ACTGCCTGGA CAAGATCAAT GAGTTCCAGT GCGAGTGCCC CACGGGCTTC 
ACTGGACACC TCTGCCAGTA TGACGTGGAT GAGTGTGCCA GCACCCCCTG CAAGAATGGT 
GCCAAGTGCC TGGACGGACC CAACACTTAC ACCTGCGTGT GCACAGAAGG GTATACGGGG 
ATGCACTGCG AGGTGGACAT TGATGAGTGC GACCCCGACC CCTGCCACTA CGGCTCCTGC 
AAGGACGGCG TCGCCACCTT CACCTGCCTC TGCCGCCCGG GCTACACGGG CCACCACTGC 
GAGACCAACA TCAACGAATG CTCCAGCCAG CCCTGCCGCC ACGGGGGCAC CTGCCAGGAC 
CGCGACAACG CCTACCTCTG CTTCTGTCTC AAGGGGACCA CAGGACCCAA CTGCGAGATC 
AACCTGGACG ACTGTGCCAG CAGCCCCTGC GACTCGGGCA CCTGTCTGGA CAAGATTGAC 
GGCTACGAGT GTGCCTGTGA GCCGGGCTAC ACAGGGAGCA TGTGCAACGT CAACATCGAT 
GAGTGTGCGG GCAACCCCTG CCACAACGGG GGCACCTGCC AGGACGGCAT CAACGGCTTC 
ACCTGCCGCT GCCCCGAGGG CTACCACGAC CCCACCTGCC TGTCTGAGGT CAATGAGTGC 
AACAGCAACC CCTGTGTCCA CGGGGCGTGC CGGGACAGCC TCAATGGGTA CAAGTGCGAC 
TGTGACCCTG GGTGGAGCGG GACCAACTGT GACATCAACA ACAATGAGTG TGAGTCCAAC 
CCTTGTGTCA ACGGTGGTAC CTGCAAAGAC ATGACCAGTG GCTACGTGTG CACCTGCCGG 
GAGGGCTTCA GTGGTCCCAA CTGCCAGACC AACATCAACG AGTGTGCATC CAACCCGTGT 
CTGAACCAGG GCACGTGTAT TGACGATGTT GCGGGGTACA AGTGCAACTG CCTGCTTCCC 
TACACAGGTG CCACGTGCGA GGTGGTGCTG GCCCCGTGTG CCCCCAGCCC CTGCAGAAAC 
GGTGGGGAGT GCAGGGAATC TGAGGACTAT GAGAGCTTCT CCTGCGTCTG CCCCACAGGC 
TGGCAAGGGC AGACCTGTGA GGTCGACATC AATGAGTGTG TTATGAGCCC GTGCCGGCAC 
GGCGCGTCCT GCCGGAACAC CCATGGCGGC TACCGCTGCC ACTGCCAGGC CGGCTACAGC 
GGGCGCAACT GCGAGACGGA CATCGACGAC TGCCGGCCCA ACCCGTGTCA CAACGGCGGC 
TCCTGCACAG ACGGCATCAA CACGGCCTTC TGCGATTGCC TGCCCGGCTT CCAGGGCACT 
TTCTGTGAGG AGGACATCAA CGAGTGTGCC AGTGACCCCT GCCGCAATGG GGCCAACTGC 
ACGGACTGCG TGGACAGCTA CACGTGCACC TGTCCCGCGG GCTTCAGCGG GATCCACTGT 
GAGAACAACA CGCCCGACTG CACGGAGAGC TCCTGCTTCA ATGGTGGCAC CTGCGTGGAC 
GGAATCAACT CGTTCACCTG CCTGTGTCCA CCTGGCTTCA CGGGCAGCTA TTGCCAGCAC 
GATGTCAACG AGTGCGACTC ACAGCCCTGC CTGCACGGCG GCACCTGTCA GGATGGCTGC 
GGCTCCTACA GGTGCACCTG CCCCCAGGGC TACACCGGCC CCAACTGCCA GAACCTCGTG 
CACTGGTGTG ACTCCTCACC CTGCAAGAAT GGTGGCAAAT GCTGGCAGAC CCACACCCAG 
TACCGCTGCG AGTGCCCCAG TGGCTGGACC GGCCTTTACT GTGATGTGCC CAGCGTGTCC 
TGTGAGGTGG CTGCGCAGCG ACAAGGCGTT GATGTTGCCC GGCTGTGCCA GCATGGAGGG 
CTCTGTGTGG ATGCGGGCAA CACACACCAC TGCCGTTGCC AGGCAGGCTA CACGGGCAGC 
TACTGTGAGG ACCTGGTGGA CGAGTGCTTA CCCAGCCCCT GCCAGAACGG GGCCACCTGC 
ACAGACTACC TGGGCGGCTA CTCCTGCAAG TGCGTGGCCG GCTACCACGG GGTGAACTGC 
TCTGAGGAGA TTGACGAGTG CCTGTCACAC CCCTGCCAGA ATGGGGGCAC CTGCCTTGAC 
CTCCCCAACA CCTACAAGTG CTCCTGCCCA CGGGGCACAC AGGGTGTGCA CTGTGAGATC 
AACGTGGACG ACTGCAACCC CCCCGTTGAC CCCGTGTCCC GGAGCCCCAA GTGCTTCAAC 
AATGGCACCT GCGTGGACCA GGTGGGCGGC TACAGCTGTA CCTGCCCGCC GGGCTTCGTG 
GGTGAGCGCT GCGAGGGGGA TGTCAACGAG TGCCTCTCCA ATCCCTGCGA CGCCCGTGGC 
ACCCAGAACT GCGTGCAGCG CGTCAACGAC TTCCACTGCG AGTGCCGTGC CGGTCACACC 
GGGCGCCGCT GCGAGTCAGT CATCAACGGC TGCAAGGGCA AGCCCTGCAA GAATGGGGGC 
ACCTGTGCCG TGGCCTCCAA CACCGCCCGT GGATTCATCT GCAAGTGCCC TGCGGGCTTC 
GAGGGCGCCA CGTGCGAGAA TGACGCTCGC ACCTGCGGCA GCCTGCGCTG CCTCAACGGT 
GGCACGTGCA TCTCTGGCCC ACGCAGCCCC ACCTGCCTGT GCCTGGGCCC CTTCACGGGC 
CCTGAGTGCC AGTTCCCGGC CAGCAGCCCC TGCCTGGGCG GCAACCCCTG CTACAACCAG 
GGGACCTGTG AGCCCACGTC GGAGAGCCCC TTCTACCGTT GCCTGTGCCC CGCCAAATTC 
AACGGGCTCT TGTGCCACAT CCTGGACTAC AGCTTCGGGG GCGGGGCCGG GCGCGACATC 
CCCCCGCCGC AGATCGAGGA GGCGTGCGAG CTGCCCGAGT GCCAGGAGGA CGCGGGCAAC 
AAGGTCTGCA GCCTGCAGTG CAACAACCAC GCGTGCGGCT GGGACGGCGG CGACTGCTCC 
CTCAACTTCA ATGACCCCTG GAAGAACTGC ACGCAGTCCC TGCAGTGCTG GAAGTACTTC 
AGCGACGGCC ACTGTGACAG CCAGTGCAAC TCGGCCGGCT GCCTCTTCGA TGGCTTCGAC 
TGCCAGCGCG CGGAAGGCCA GTGCAACCCC CTGTATGACC AGTACTGCAA GGACCACTTC 
AGCGACGGGC ACTGCGACCA GGGCTGCAAC AGCGCGGAGT GCGAGTGGGA CGGGCTGGAC 
TGCGCGGAGC ATGTGCCGGA GAGGCTGGCG GCGGGCACGC TGGTGGTGGT GGTGCTGATG 
CCGCCGGAGC AGCTGCGCAA CAGCTCCTTC CACTTCCTGC GGGAGCTCAG CCGGGTGCTG 
CACACCAACG TGGTCTTCAA GCGCGACGCG CACGGCCAGC AGATGATCTT CCCCTACTAC 
GGCCACGAGG AAGAGCTTCG CAAGCACCCC ATCAAGCGTG CCGCCGAGGG CTGGGCCACC 
CCCGAGGCCT TGCTGGGCCA GGTGAAGGCC TCGCTGCTCC CTGGTGGCAG TGGTGGGCGG 
CGGCGGAGGG AGCTGGACCC CATGGACGTC CGCGGCTCCA TCGTCTACCT GGAGATTGAC 
AACCGGCAGT GTGTGCAGGC CTCCTCGCAG TGCTTCCAGA GCGCCACCGA CGTGGCCGCG 
TTCCTGGGGG CGCTCGCCTC GCTGGGCAGC CTCAACATCC CCTACAAGAT CGAGGCCGTG 
CAGAGTGAGA CCGTGGAGCC GCCCCCGCCA GCGCAGCTGC ACTTCATGTA CGTGGCGGCG 
GCCGCCTTTG TGCTCCTGTT CTTCGTGGGC TGCGGGGTGC TGCTGTCCCG CAAGCGCCGG 
CGGCAGCATG GCCAACTCTG GTTCCCTGAG GGCTTCAAGG TGTCCGAGGC CAGCAAGAAG 
AAGCGGCGGG AGCCCCTCGG CGAGGACTCT GTGGGCCTCA AGCCCCTGAA GAATGCCTCA 
GATGGTGCCC TCATGGACGA CAACCAGAAT GAGTGGGGGG ATGAGGACCT GGAGACCAAG 
AAGTTCCGGT TCGAGGAGCC CGTGGTTCTG CCTGACCTGG ACGACCAGAC AGACCACCGG 
CAGTGGACTC AGCAGCACCT GGATGCCGCT GACCTGCGCA TGTCTGCCAT GGCCCCCACG 
CCGCCCCAGG GTGAGGTTGA CGCCGACTGC ATGGACGTCA ATGTCCGCGG GCCTGACGGC 
TTCACCCCCC TCATGATCGC CTCCTGCAGT GGGGGCGGCC TGGAGACGGG CAACAGCGAG 
GAGGAGGAGG ACGCGCCGGC CGTCATCTCC GACTTCATCT ACCAGGGCGC CAGCCTGCAC 
AACCAGACCG ACCGCACGGG CGAGACCGCC TTGCACCTGG CTGCCCGCTA CTCACGCTCT 
GATGCCGCCA AGCGCCTGCT GGAGGCCAGT GCAGACGCCA ACATCCAGGA CAACATGGGC 
CGCACCCCGC TGCACGCAGC TGTGTCTGCC GACGCACAAG GTGTCTTCCA GATCCTGATC 
CGGAACCGAG CCACGGACCT GGATGCCCGC ATGCACGACG GCACGACGCC GCTGATCCTG 
GCTGCCCGCC TGGCTGTGGA GGGCATGCTG GAGGACCTCA TCAACTCTCA CGCCGATGTC 
AATGCTGTGG ACGACTTGGG CAAGTCCGCC CTGCACTGGG CCGCCGCCGT GAACAACGTG 
GACGCCGCGG TCGTGCTCCT GAAGAACGGG GCCAATAAAG ACATGCAGAA CAACAAGGAG 
GAGACACCCC TGTTTCTGGC CGCCCGGGAG GGGAGCTACG AGACCGCCAA GGTGCTGCTG 
GACCACTTTG CCAACCGGGA CATCACGGAC CATATGGATC GCCTGCCACG TGACATCGCA 
CAGGAGCGCA TGCACCACGA CATCGTGAGG CTGCTGGACG AGTACAACCT GGTGCGCAGC 
CCACAGCTGC ACGGAGCCAC ACTGGGGGGC ACGCCCACCC TGTCACCCCC GCTCTGCTCA 
CCCAACGGCT ACCTGGGCAG CCTCAAGCCC GGCGTGCAGG GTAAGAAGGT CCGCAAGCCC 
AGCAGCAAAG GCCTGGCCTG TGGGAGCAAG GAGGCCAAGG ACCTCAAGGC GCGGAGGAAG 
AAGTCCCAGG ACGGCAAGGG CTGCCTGCTG GACAGCTCCG GCATGCTCTC ACCCGTGGAC 
TCCCTGGAGT CGCCCCACGG CTACCTGTCG GACGTGGCCT CGCCGCCACT GCTGCCCTCT 
CCGTTCCAGC AGTCTCCGTC CGTGCCCCTC AACCACCTGC CCGGTATGCC CGACACCCAC 
CTGGGCATCG GGCACCTGAA CGTGGCGGCC AAGCCAGAGA TGGCAACACT GGGTGGGGGC 
GGCCGGCTGG CCTTCGAGGC CGGCCCCCCG CGTCTCTCCC ACCTGCCCGT GGCCTCCAGC 
ACCAGCACCA TCCTGGGCTG CAGCAGTGGA GGAGCCATGA ATTTCACCGT GGGTGGGTCC 
ACCAGTTTGA ATGGTCAATG CGAGTGGCTG TCCCGGCTGC AGAGCGGCAT GGTGCCGAAC 
CAATACAACC CTCTGCGGGG GAGCGTGGCA CCAGGCCCCC TAAGCACGCA GGCCCCCTCC 
CTGCAGCATG GCATGGTGGG CCCGCTGCAC AGTAGCCTTG CTGCCAGCGC CCTGTCCCAG 
ATGATGAGCT ACCAGGGCCT GCCCAGTACC CGGCTGGCCA CCCAGCCTCA CCTGGTGCAG 
ACCCAGCAGG TGCAGCCGCA AAACTTACAG ATGCAGCAGC AGAACCTGCA GCCACCAAAC 
ATCCAGCAGC AGCAAAATCT GCAGCCGCCA CCGCCACCAC CACAGCCGCA CCTTGGCGTG 
AGCTCAGCAG CCAGCGGCCA CCTGGGCCGG AGCTTCCTGG GCGGAGAGCC GAGCCAGGCA 
GACGTGCAGC CACTAGGCCC CAGCAGCCTG GCGGTGCACA CCATTCTACC CCAGGAGAGC 
CCTGCTCTGC CCACGTCGCT GCCGTCCTCG CTGGTCCCAC CCGTGACCGC GGCCCAGTTC 
CTGACACCCC CCTCACAGCA CAGCTACTCC TCCTCGCCTG TGGACAACAC ACCCAGCCAC 
CAACTGCAGG TGCCTGAGCA CCCCTTCCTC ACCCCGTCCC CCGAGTCCCC TGACCAGTGG 
TCCAGCTCGT CCCCGCATTC CAACGTCTCC GACTGGTCTG AGGGCGTCTC CAGCCCGCCC 
ACCAGCATGC AGTCCCAGAT CGCCCGCATT CCGGAGGCCT TCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH1 ( XM_008005609.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008003800.1 Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X2, mRNA.

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CloneID	OCh98791
Clone ID Related Accession (Same CDS sequence)	XM_008005609.1
Accession Version	XM_008005609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	neurogenic locus notch homolog protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735255.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGTTTT TCCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC GGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTTGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCAACCC CTGCAAGAAC ACCGGGACAT GCCACGTGGT GGACCGTGGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTCCC CTGGGCTTCT CTGGGCCCCT CTGCCTCACG 
CCCCTGGACA ACGCTTGCCT CACCAACCCC TGCCGCAATG GGGGCACCTG CGACCTGCTC 
ACGCTCACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCCT GCGCCTCCAA CCCCTGTGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CACGGTCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGCGGCC AGAACCCGGG GCTCTGCCGC CATGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACCGGGCCCA ACTGTGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG TGACGTCACC 
CACGAGTGCA CCTGCCTGCC AGGCTTCACC GGCCAGAACT GCGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAATGGGGGC GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGACGTGGA TGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACCGGTGAG GACTGCAGCG AGAACATCGA TGACTGCGCC 
AGCGCCGCCT GCTTCCACGG TGCCACCTGC CACGACCGCG TGGCCTCCTT CTACTGCGAG 
TGTCCCCACG GCCGCACAGG TCTGCTGTGT CACCTCAACG ATGCGTGTAT CAGTAATCCC 
TGCAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAACG GCAAGGCCAT CTGCACGTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGATGTGG ACGAGTGCTC GCTGGGTGCC 
AACCCCTGTG AGCATGCGGG CAAGTGCATC AACACACTGG GCTCCTTCGA GTGCCAGTGC 
CTGCAGGGCT ACACGGGCCC CCGCTGCGAG ATCGACGTCA ACGAGTGTGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTTCACTG GACACCTCTG CCAGTATGAC GTGGATGAGT GTGCCAGCAC CCCCTGCAAG 
AATGGTGCCA AGTGCCTGGA CGGACCCAAC ACTTACACCT GCGTGTGCAC AGAAGGGTAT 
ACGGGGATGC ACTGCGAGGT GGACATTGAT GAGTGCGACC CCGACCCCTG CCACTACGGC 
TCCTGCAAGG ACGGCGTCGC CACCTTCACC TGCCTCTGCC GCCCGGGCTA CACGGGCCAC 
CACTGCGAGA CCAACATCAA CGAATGCTCC AGCCAGCCCT GCCGCCACGG GGGCACCTGC 
CAGGACCGCG ACAACGCCTA CCTCTGCTTC TGTCTCAAGG GGACCACAGG ACCCAACTGC 
GAGATCAACC TGGACGACTG TGCCAGCAGC CCCTGCGACT CGGGCACCTG TCTGGACAAG 
ATTGACGGCT ACGAGTGTGC CTGTGAGCCG GGCTACACAG GGAGCATGTG CAACGTCAAC 
ATCGATGAGT GTGCGGGCAA CCCCTGCCAC AACGGGGGCA CCTGCCAGGA CGGCATCAAC 
GGCTTCACCT GCCGCTGCCC CGAGGGCTAC CACGACCCCA CCTGCCTGTC TGAGGTCAAT 
GAGTGCAACA GCAACCCCTG TGTCCACGGG GCGTGCCGGG ACAGCCTCAA TGGGTACAAG 
TGCGACTGTG ACCCTGGGTG GAGCGGGACC AACTGTGACA TCAACAACAA TGAGTGTGAG 
TCCAACCCTT GTGTCAACGG TGGTACCTGC AAAGACATGA CCAGTGGCTA CGTGTGCACC 
TGCCGGGAGG GCTTCAGTGG TCCCAACTGC CAGACCAACA TCAACGAGTG TGCATCCAAC 
CCGTGTCTGA ACCAGGGCAC GTGTATTGAC GATGTTGCGG GGTACAAGTG CAACTGCCTG 
CTTCCCTACA CAGGTGCCAC GTGCGAGGTG GTGCTGGCCC CGTGTGCCCC CAGCCCCTGC 
AGAAACGGTG GGGAGTGCAG GGAATCTGAG GACTATGAGA GCTTCTCCTG CGTCTGCCCC 
ACAGGCTGGC AAGGGCAGAC CTGTGAGGTC GACATCAATG AGTGTGTTAT GAGCCCGTGC 
CGGCACGGCG CGTCCTGCCG GAACACCCAT GGCGGCTACC GCTGCCACTG CCAGGCCGGC 
TACAGCGGGC GCAACTGCGA GACGGACATC GACGACTGCC GGCCCAACCC GTGTCACAAC 
GGCGGCTCCT GCACAGACGG CATCAACACG GCCTTCTGCG ATTGCCTGCC CGGCTTCCAG 
GGCACTTTCT GTGAGGAGGA CATCAACGAG TGTGCCAGTG ACCCCTGCCG CAATGGGGCC 
AACTGCACGG ACTGCGTGGA CAGCTACACG TGCACCTGTC CCGCGGGCTT CAGCGGGATC 
CACTGTGAGA ACAACACGCC CGACTGCACG GAGAGCTCCT GCTTCAATGG TGGCACCTGC 
GTGGACGGAA TCAACTCGTT CACCTGCCTG TGTCCACCTG GCTTCACGGG CAGCTATTGC 
CAGCACGATG TCAACGAGTG CGACTCACAG CCCTGCCTGC ACGGCGGCAC CTGTCAGGAT 
GGCTGCGGCT CCTACAGGTG CACCTGCCCC CAGGGCTACA CCGGCCCCAA CTGCCAGAAC 
CTCGTGCACT GGTGTGACTC CTCACCCTGC AAGAATGGTG GCAAATGCTG GCAGACCCAC 
ACCCAGTACC GCTGCGAGTG CCCCAGTGGC TGGACCGGCC TTTACTGTGA TGTGCCCAGC 
GTGTCCTGTG AGGTGGCTGC GCAGCGACAA GGCGTTGATG TTGCCCGGCT GTGCCAGCAT 
GGAGGGCTCT GTGTGGATGC GGGCAACACA CACCACTGCC GTTGCCAGGC AGGCTACACG 
GGCAGCTACT GTGAGGACCT GGTGGACGAG TGCTTACCCA GCCCCTGCCA GAACGGGGCC 
ACCTGCACAG ACTACCTGGG CGGCTACTCC TGCAAGTGCG TGGCCGGCTA CCACGGGGTG 
AACTGCTCTG AGGAGATTGA CGAGTGCCTG TCACACCCCT GCCAGAATGG GGGCACCTGC 
CTTGACCTCC CCAACACCTA CAAGTGCTCC TGCCCACGGG GCACACAGGG TGTGCACTGT 
GAGATCAACG TGGACGACTG CAACCCCCCC GTTGACCCCG TGTCCCGGAG CCCCAAGTGC 
TTCAACAATG GCACCTGCGT GGACCAGGTG GGCGGCTACA GCTGTACCTG CCCGCCGGGC 
TTCGTGGGTG AGCGCTGCGA GGGGGATGTC AACGAGTGCC TCTCCAATCC CTGCGACGCC 
CGTGGCACCC AGAACTGCGT GCAGCGCGTC AACGACTTCC ACTGCGAGTG CCGTGCCGGT 
CACACCGGGC GCCGCTGCGA GTCAGTCATC AACGGCTGCA AGGGCAAGCC CTGCAAGAAT 
GGGGGCACCT GTGCCGTGGC CTCCAACACC GCCCGTGGAT TCATCTGCAA GTGCCCTGCG 
GGCTTCGAGG GCGCCACGTG CGAGAATGAC GCTCGCACCT GCGGCAGCCT GCGCTGCCTC 
AACGGTGGCA CGTGCATCTC TGGCCCACGC AGCCCCACCT GCCTGTGCCT GGGCCCCTTC 
ACGGGCCCTG AGTGCCAGTT CCCGGCCAGC AGCCCCTGCC TGGGCGGCAA CCCCTGCTAC 
AACCAGGGGA CCTGTGAGCC CACGTCGGAG AGCCCCTTCT ACCGTTGCCT GTGCCCCGCC 
AAATTCAACG GGCTCTTGTG CCACATCCTG GACTACAGCT TCGGGGGCGG GGCCGGGCGC 
GACATCCCCC CGCCGCAGAT CGAGGAGGCG TGCGAGCTGC CCGAGTGCCA GGAGGACGCG 
GGCAACAAGG TCTGCAGCCT GCAGTGCAAC AACCACGCGT GCGGCTGGGA CGGCGGCGAC 
TGCTCCCTCA ACTTCAATGA CCCCTGGAAG AACTGCACGC AGTCCCTGCA GTGCTGGAAG 
TACTTCAGCG ACGGCCACTG TGACAGCCAG TGCAACTCGG CCGGCTGCCT CTTCGATGGC 
TTCGACTGCC AGCGCGCGGA AGGCCAGTGC AACCCCCTGT ATGACCAGTA CTGCAAGGAC 
CACTTCAGCG ACGGGCACTG CGACCAGGGC TGCAACAGCG CGGAGTGCGA GTGGGACGGG 
CTGGACTGCG CGGAGCATGT GCCGGAGAGG CTGGCGGCGG GCACGCTGGT GGTGGTGGTG 
CTGATGCCGC CGGAGCAGCT GCGCAACAGC TCCTTCCACT TCCTGCGGGA GCTCAGCCGG 
GTGCTGCACA CCAACGTGGT CTTCAAGCGC GACGCGCACG GCCAGCAGAT GATCTTCCCC 
TACTACGGCC ACGAGGAAGA GCTTCGCAAG CACCCCATCA AGCGTGCCGC CGAGGGCTGG 
GCCACCCCCG AGGCCTTGCT GGGCCAGGTG AAGGCCTCGC TGCTCCCTGG TGGCAGTGGT 
GGGCGGCGGC GGAGGGAGCT GGACCCCATG GACGTCCGCG GCTCCATCGT CTACCTGGAG 
ATTGACAACC GGCAGTGTGT GCAGGCCTCC TCGCAGTGCT TCCAGAGCGC CACCGACGTG 
GCCGCGTTCC TGGGGGCGCT CGCCTCGCTG GGCAGCCTCA ACATCCCCTA CAAGATCGAG 
GCCGTGCAGA GTGAGACCGT GGAGCCGCCC CCGCCAGCGC AGCTGCACTT CATGTACGTG 
GCGGCGGCCG CCTTTGTGCT CCTGTTCTTC GTGGGCTGCG GGGTGCTGCT GTCCCGCAAG 
CGCCGGCGGC AGCATGGCCA ACTCTGGTTC CCTGAGGGCT TCAAGGTGTC CGAGGCCAGC 
AAGAAGAAGC GGCGGGAGCC CCTCGGCGAG GACTCTGTGG GCCTCAAGCC CCTGAAGAAT 
GCCTCAGATG GTGCCCTCAT GGACGACAAC CAGAATGAGT GGGGGGATGA GGACCTGGAG 
ACCAAGAAGT TCCGGTTCGA GGAGCCCGTG GTTCTGCCTG ACCTGGACGA CCAGACAGAC 
CACCGGCAGT GGACTCAGCA GCACCTGGAT GCCGCTGACC TGCGCATGTC TGCCATGGCC 
CCCACGCCGC CCCAGGGTGA GGTTGACGCC GACTGCATGG ACGTCAATGT CCGCGGGCCT 
GACGGCTTCA CCCCCCTCAT GATCGCCTCC TGCAGTGGGG GCGGCCTGGA GACGGGCAAC 
AGCGAGGAGG AGGAGGACGC GCCGGCCGTC ATCTCCGACT TCATCTACCA GGGCGCCAGC 
CTGCACAACC AGACCGACCG CACGGGCGAG ACCGCCTTGC ACCTGGCTGC CCGCTACTCA 
CGCTCTGATG CCGCCAAGCG CCTGCTGGAG GCCAGTGCAG ACGCCAACAT CCAGGACAAC 
ATGGGCCGCA CCCCGCTGCA CGCAGCTGTG TCTGCCGACG CACAAGGTGT CTTCCAGATC 
CTGATCCGGA ACCGAGCCAC GGACCTGGAT GCCCGCATGC ACGACGGCAC GACGCCGCTG 
ATCCTGGCTG CCCGCCTGGC TGTGGAGGGC ATGCTGGAGG ACCTCATCAA CTCTCACGCC 
GATGTCAATG CTGTGGACGA CTTGGGCAAG TCCGCCCTGC ACTGGGCCGC CGCCGTGAAC 
AACGTGGACG CCGCGGTCGT GCTCCTGAAG AACGGGGCCA ATAAAGACAT GCAGAACAAC 
AAGGAGGAGA CACCCCTGTT TCTGGCCGCC CGGGAGGGGA GCTACGAGAC CGCCAAGGTG 
CTGCTGGACC ACTTTGCCAA CCGGGACATC ACGGACCATA TGGATCGCCT GCCACGTGAC 
ATCGCACAGG AGCGCATGCA CCACGACATC GTGAGGCTGC TGGACGAGTA CAACCTGGTG 
CGCAGCCCAC AGCTGCACGG AGCCACACTG GGGGGCACGC CCACCCTGTC ACCCCCGCTC 
TGCTCACCCA ACGGCTACCT GGGCAGCCTC AAGCCCGGCG TGCAGGGTAA GAAGGTCCGC 
AAGCCCAGCA GCAAAGGCCT GGCCTGTGGG AGCAAGGAGG CCAAGGACCT CAAGGCGCGG 
AGGAAGAAGT CCCAGGACGG CAAGGGCTGC CTGCTGGACA GCTCCGGCAT GCTCTCACCC 
GTGGACTCCC TGGAGTCGCC CCACGGCTAC CTGTCGGACG TGGCCTCGCC GCCACTGCTG 
CCCTCTCCGT TCCAGCAGTC TCCGTCCGTG CCCCTCAACC ACCTGCCCGG TATGCCCGAC 
ACCCACCTGG GCATCGGGCA CCTGAACGTG GCGGCCAAGC CAGAGATGGC AACACTGGGT 
GGGGGCGGCC GGCTGGCCTT CGAGGCCGGC CCCCCGCGTC TCTCCCACCT GCCCGTGGCC 
TCCAGCACCA GCACCATCCT GGGCTGCAGC AGTGGAGGAG CCATGAATTT CACCGTGGGT 
GGGTCCACCA GTTTGAATGG TCAATGCGAG TGGCTGTCCC GGCTGCAGAG CGGCATGGTG 
CCGAACCAAT ACAACCCTCT GCGGGGGAGC GTGGCACCAG GCCCCCTAAG CACGCAGGCC 
CCCTCCCTGC AGCATGGCAT GGTGGGCCCG CTGCACAGTA GCCTTGCTGC CAGCGCCCTG 
TCCCAGATGA TGAGCTACCA GGGCCTGCCC AGTACCCGGC TGGCCACCCA GCCTCACCTG 
GTGCAGACCC AGCAGGTGCA GCCGCAAAAC TTACAGATGC AGCAGCAGAA CCTGCAGCCA 
CCAAACATCC AGCAGCAGCA AAATCTGCAG CCGCCACCGC CACCACCACA GCCGCACCTT 
GGCGTGAGCT CAGCAGCCAG CGGCCACCTG GGCCGGAGCT TCCTGGGCGG AGAGCCGAGC 
CAGGCAGACG TGCAGCCACT AGGCCCCAGC AGCCTGGCGG TGCACACCAT TCTACCCCAG 
GAGAGCCCTG CTCTGCCCAC GTCGCTGCCG TCCTCGCTGG TCCCACCCGT GACCGCGGCC 
CAGTTCCTGA CACCCCCCTC ACAGCACAGC TACTCCTCCT CGCCTGTGGA CAACACACCC 
AGCCACCAAC TGCAGGTGCC TGAGCACCCC TTCCTCACCC CGTCCCCCGA GTCCCCTGAC 
CAGTGGTCCA GCTCGTCCCC GCATTCCAAC GTCTCCGACT GGTCTGAGGG CGTCTCCAGC 
CCGCCCACCA GCATGCAGTC CCAGATCGCC CGCATTCCGG AGGCCTTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008003800.1
CDS	256..7809
Translation	MPPLLAPLLCLAFFPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSNPCKNTGTCHVVDRGGVADYACSCPLGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQNPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECTCLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGMHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNVNIDECAGNPCHNGGTCQDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRESEDYESFSCVCPTGWQGQTCEVDINECVMSPCRHGASCRNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFQGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECLPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPQIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGHEEELRKHPIKRAAEGWATPEALLGQVKASLLPGGSGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNKEETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGATLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMATLGGGGRLAFEAGPPRLSHLPVASSTSTILGCSSGGAMNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPPNIQQQQNLQPPPPPPQPHLGVSSAASGHLGRSFLGGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK

Target ORF information:

RefSeq Version	XM_008005609.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus notch 1 (NOTCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008005609.1

ORF Insert Sequence:

ATGCCGCCGC TCCTGGCGCC CCTGCTCTGC CTGGCGTTTT TCCCCGCGCT CGCCGCACGA 
GGCCCGCGAT GCTCCCAGCC GGGTGAGACC TGCCTGAATG GCGGGAAGTG TGAAGCGGCC 
AATGGCACGG AGGCCTGCGT CTGTGGCGGG GCCTTTGTGG GCCCGCGATG CCAGGACCCC 
AACCCGTGCC TCAGCAACCC CTGCAAGAAC ACCGGGACAT GCCACGTGGT GGACCGTGGA 
GGCGTGGCAG ACTATGCCTG CAGCTGTCCC CTGGGCTTCT CTGGGCCCCT CTGCCTCACG 
CCCCTGGACA ACGCTTGCCT CACCAACCCC TGCCGCAATG GGGGCACCTG CGACCTGCTC 
ACGCTCACGG AGTACAAGTG CCGCTGCCCG CCCGGCTGGT CAGGGAAATC GTGCCAGCAG 
GCTGACCCCT GCGCCTCCAA CCCCTGTGCC AACGGTGGCC AGTGCCTGCC CTTCGAGGCC 
TCCTACATCT GCCACTGCCC ACCCAGCTTC CACGGTCCCA CCTGCCGGCA GGATGTCAAC 
GAGTGCGGCC AGAACCCGGG GCTCTGCCGC CATGGAGGCA CCTGCCACAA CGAGGTCGGC 
TCCTACCGCT GCGTCTGCCG CGCCACCCAC ACCGGGCCCA ACTGTGAGCG GCCCTACGTG 
CCCTGCAGCC CCTCGCCCTG CCAGAACGGG GGCACCTGCC GCCCCACGGG TGACGTCACC 
CACGAGTGCA CCTGCCTGCC AGGCTTCACC GGCCAGAACT GCGAGGAAAA TATCGACGAT 
TGTCCAGGAA ACAACTGCAA GAATGGGGGC GCCTGTGTGG ACGGCGTGAA CACCTACAAC 
TGCCGCTGCC CGCCAGAGTG GACAGGTCAG TACTGTACCG AGGACGTGGA TGAGTGCCAG 
CTGATGCCAA ATGCCTGCCA GAACGGCGGG ACCTGCCACA ACACCCACGG TGGCTACAAC 
TGCGTGTGTG TCAACGGCTG GACCGGTGAG GACTGCAGCG AGAACATCGA TGACTGCGCC 
AGCGCCGCCT GCTTCCACGG TGCCACCTGC CACGACCGCG TGGCCTCCTT CTACTGCGAG 
TGTCCCCACG GCCGCACAGG TCTGCTGTGT CACCTCAACG ATGCGTGTAT CAGTAATCCC 
TGCAACGAGG GCTCCAACTG CGACACCAAC CCTGTCAACG GCAAGGCCAT CTGCACGTGC 
CCCTCGGGGT ACACGGGCCC GGCCTGCAGC CAGGATGTGG ACGAGTGCTC GCTGGGTGCC 
AACCCCTGTG AGCATGCGGG CAAGTGCATC AACACACTGG GCTCCTTCGA GTGCCAGTGC 
CTGCAGGGCT ACACGGGCCC CCGCTGCGAG ATCGACGTCA ACGAGTGTGT CTCGAACCCG 
TGCCAGAACG ACGCCACCTG CCTGGACCAG ATCGGGGAGT TCCAGTGCAT CTGCATGCCC 
GGCTTCACTG GACACCTCTG CCAGTATGAC GTGGATGAGT GTGCCAGCAC CCCCTGCAAG 
AATGGTGCCA AGTGCCTGGA CGGACCCAAC ACTTACACCT GCGTGTGCAC AGAAGGGTAT 
ACGGGGATGC ACTGCGAGGT GGACATTGAT GAGTGCGACC CCGACCCCTG CCACTACGGC 
TCCTGCAAGG ACGGCGTCGC CACCTTCACC TGCCTCTGCC GCCCGGGCTA CACGGGCCAC 
CACTGCGAGA CCAACATCAA CGAATGCTCC AGCCAGCCCT GCCGCCACGG GGGCACCTGC 
CAGGACCGCG ACAACGCCTA CCTCTGCTTC TGTCTCAAGG GGACCACAGG ACCCAACTGC 
GAGATCAACC TGGACGACTG TGCCAGCAGC CCCTGCGACT CGGGCACCTG TCTGGACAAG 
ATTGACGGCT ACGAGTGTGC CTGTGAGCCG GGCTACACAG GGAGCATGTG CAACGTCAAC 
ATCGATGAGT GTGCGGGCAA CCCCTGCCAC AACGGGGGCA CCTGCCAGGA CGGCATCAAC 
GGCTTCACCT GCCGCTGCCC CGAGGGCTAC CACGACCCCA CCTGCCTGTC TGAGGTCAAT 
GAGTGCAACA GCAACCCCTG TGTCCACGGG GCGTGCCGGG ACAGCCTCAA TGGGTACAAG 
TGCGACTGTG ACCCTGGGTG GAGCGGGACC AACTGTGACA TCAACAACAA TGAGTGTGAG 
TCCAACCCTT GTGTCAACGG TGGTACCTGC AAAGACATGA CCAGTGGCTA CGTGTGCACC 
TGCCGGGAGG GCTTCAGTGG TCCCAACTGC CAGACCAACA TCAACGAGTG TGCATCCAAC 
CCGTGTCTGA ACCAGGGCAC GTGTATTGAC GATGTTGCGG GGTACAAGTG CAACTGCCTG 
CTTCCCTACA CAGGTGCCAC GTGCGAGGTG GTGCTGGCCC CGTGTGCCCC CAGCCCCTGC 
AGAAACGGTG GGGAGTGCAG GGAATCTGAG GACTATGAGA GCTTCTCCTG CGTCTGCCCC 
ACAGGCTGGC AAGGGCAGAC CTGTGAGGTC GACATCAATG AGTGTGTTAT GAGCCCGTGC 
CGGCACGGCG CGTCCTGCCG GAACACCCAT GGCGGCTACC GCTGCCACTG CCAGGCCGGC 
TACAGCGGGC GCAACTGCGA GACGGACATC GACGACTGCC GGCCCAACCC GTGTCACAAC 
GGCGGCTCCT GCACAGACGG CATCAACACG GCCTTCTGCG ATTGCCTGCC CGGCTTCCAG 
GGCACTTTCT GTGAGGAGGA CATCAACGAG TGTGCCAGTG ACCCCTGCCG CAATGGGGCC 
AACTGCACGG ACTGCGTGGA CAGCTACACG TGCACCTGTC CCGCGGGCTT CAGCGGGATC 
CACTGTGAGA ACAACACGCC CGACTGCACG GAGAGCTCCT GCTTCAATGG TGGCACCTGC 
GTGGACGGAA TCAACTCGTT CACCTGCCTG TGTCCACCTG GCTTCACGGG CAGCTATTGC 
CAGCACGATG TCAACGAGTG CGACTCACAG CCCTGCCTGC ACGGCGGCAC CTGTCAGGAT 
GGCTGCGGCT CCTACAGGTG CACCTGCCCC CAGGGCTACA CCGGCCCCAA CTGCCAGAAC 
CTCGTGCACT GGTGTGACTC CTCACCCTGC AAGAATGGTG GCAAATGCTG GCAGACCCAC 
ACCCAGTACC GCTGCGAGTG CCCCAGTGGC TGGACCGGCC TTTACTGTGA TGTGCCCAGC 
GTGTCCTGTG AGGTGGCTGC GCAGCGACAA GGCGTTGATG TTGCCCGGCT GTGCCAGCAT 
GGAGGGCTCT GTGTGGATGC GGGCAACACA CACCACTGCC GTTGCCAGGC AGGCTACACG 
GGCAGCTACT GTGAGGACCT GGTGGACGAG TGCTTACCCA GCCCCTGCCA GAACGGGGCC 
ACCTGCACAG ACTACCTGGG CGGCTACTCC TGCAAGTGCG TGGCCGGCTA CCACGGGGTG 
AACTGCTCTG AGGAGATTGA CGAGTGCCTG TCACACCCCT GCCAGAATGG GGGCACCTGC 
CTTGACCTCC CCAACACCTA CAAGTGCTCC TGCCCACGGG GCACACAGGG TGTGCACTGT 
GAGATCAACG TGGACGACTG CAACCCCCCC GTTGACCCCG TGTCCCGGAG CCCCAAGTGC 
TTCAACAATG GCACCTGCGT GGACCAGGTG GGCGGCTACA GCTGTACCTG CCCGCCGGGC 
TTCGTGGGTG AGCGCTGCGA GGGGGATGTC AACGAGTGCC TCTCCAATCC CTGCGACGCC 
CGTGGCACCC AGAACTGCGT GCAGCGCGTC AACGACTTCC ACTGCGAGTG CCGTGCCGGT 
CACACCGGGC GCCGCTGCGA GTCAGTCATC AACGGCTGCA AGGGCAAGCC CTGCAAGAAT 
GGGGGCACCT GTGCCGTGGC CTCCAACACC GCCCGTGGAT TCATCTGCAA GTGCCCTGCG 
GGCTTCGAGG GCGCCACGTG CGAGAATGAC GCTCGCACCT GCGGCAGCCT GCGCTGCCTC 
AACGGTGGCA CGTGCATCTC TGGCCCACGC AGCCCCACCT GCCTGTGCCT GGGCCCCTTC 
ACGGGCCCTG AGTGCCAGTT CCCGGCCAGC AGCCCCTGCC TGGGCGGCAA CCCCTGCTAC 
AACCAGGGGA CCTGTGAGCC CACGTCGGAG AGCCCCTTCT ACCGTTGCCT GTGCCCCGCC 
AAATTCAACG GGCTCTTGTG CCACATCCTG GACTACAGCT TCGGGGGCGG GGCCGGGCGC 
GACATCCCCC CGCCGCAGAT CGAGGAGGCG TGCGAGCTGC CCGAGTGCCA GGAGGACGCG 
GGCAACAAGG TCTGCAGCCT GCAGTGCAAC AACCACGCGT GCGGCTGGGA CGGCGGCGAC 
TGCTCCCTCA ACTTCAATGA CCCCTGGAAG AACTGCACGC AGTCCCTGCA GTGCTGGAAG 
TACTTCAGCG ACGGCCACTG TGACAGCCAG TGCAACTCGG CCGGCTGCCT CTTCGATGGC 
TTCGACTGCC AGCGCGCGGA AGGCCAGTGC AACCCCCTGT ATGACCAGTA CTGCAAGGAC 
CACTTCAGCG ACGGGCACTG CGACCAGGGC TGCAACAGCG CGGAGTGCGA GTGGGACGGG 
CTGGACTGCG CGGAGCATGT GCCGGAGAGG CTGGCGGCGG GCACGCTGGT GGTGGTGGTG 
CTGATGCCGC CGGAGCAGCT GCGCAACAGC TCCTTCCACT TCCTGCGGGA GCTCAGCCGG 
GTGCTGCACA CCAACGTGGT CTTCAAGCGC GACGCGCACG GCCAGCAGAT GATCTTCCCC 
TACTACGGCC ACGAGGAAGA GCTTCGCAAG CACCCCATCA AGCGTGCCGC CGAGGGCTGG 
GCCACCCCCG AGGCCTTGCT GGGCCAGGTG AAGGCCTCGC TGCTCCCTGG TGGCAGTGGT 
GGGCGGCGGC GGAGGGAGCT GGACCCCATG GACGTCCGCG GCTCCATCGT CTACCTGGAG 
ATTGACAACC GGCAGTGTGT GCAGGCCTCC TCGCAGTGCT TCCAGAGCGC CACCGACGTG 
GCCGCGTTCC TGGGGGCGCT CGCCTCGCTG GGCAGCCTCA ACATCCCCTA CAAGATCGAG 
GCCGTGCAGA GTGAGACCGT GGAGCCGCCC CCGCCAGCGC AGCTGCACTT CATGTACGTG 
GCGGCGGCCG CCTTTGTGCT CCTGTTCTTC GTGGGCTGCG GGGTGCTGCT GTCCCGCAAG 
CGCCGGCGGC AGCATGGCCA ACTCTGGTTC CCTGAGGGCT TCAAGGTGTC CGAGGCCAGC 
AAGAAGAAGC GGCGGGAGCC CCTCGGCGAG GACTCTGTGG GCCTCAAGCC CCTGAAGAAT 
GCCTCAGATG GTGCCCTCAT GGACGACAAC CAGAATGAGT GGGGGGATGA GGACCTGGAG 
ACCAAGAAGT TCCGGTTCGA GGAGCCCGTG GTTCTGCCTG ACCTGGACGA CCAGACAGAC 
CACCGGCAGT GGACTCAGCA GCACCTGGAT GCCGCTGACC TGCGCATGTC TGCCATGGCC 
CCCACGCCGC CCCAGGGTGA GGTTGACGCC GACTGCATGG ACGTCAATGT CCGCGGGCCT 
GACGGCTTCA CCCCCCTCAT GATCGCCTCC TGCAGTGGGG GCGGCCTGGA GACGGGCAAC 
AGCGAGGAGG AGGAGGACGC GCCGGCCGTC ATCTCCGACT TCATCTACCA GGGCGCCAGC 
CTGCACAACC AGACCGACCG CACGGGCGAG ACCGCCTTGC ACCTGGCTGC CCGCTACTCA 
CGCTCTGATG CCGCCAAGCG CCTGCTGGAG GCCAGTGCAG ACGCCAACAT CCAGGACAAC 
ATGGGCCGCA CCCCGCTGCA CGCAGCTGTG TCTGCCGACG CACAAGGTGT CTTCCAGATC 
CTGATCCGGA ACCGAGCCAC GGACCTGGAT GCCCGCATGC ACGACGGCAC GACGCCGCTG 
ATCCTGGCTG CCCGCCTGGC TGTGGAGGGC ATGCTGGAGG ACCTCATCAA CTCTCACGCC 
GATGTCAATG CTGTGGACGA CTTGGGCAAG TCCGCCCTGC ACTGGGCCGC CGCCGTGAAC 
AACGTGGACG CCGCGGTCGT GCTCCTGAAG AACGGGGCCA ATAAAGACAT GCAGAACAAC 
AAGGAGGAGA CACCCCTGTT TCTGGCCGCC CGGGAGGGGA GCTACGAGAC CGCCAAGGTG 
CTGCTGGACC ACTTTGCCAA CCGGGACATC ACGGACCATA TGGATCGCCT GCCACGTGAC 
ATCGCACAGG AGCGCATGCA CCACGACATC GTGAGGCTGC TGGACGAGTA CAACCTGGTG 
CGCAGCCCAC AGCTGCACGG AGCCACACTG GGGGGCACGC CCACCCTGTC ACCCCCGCTC 
TGCTCACCCA ACGGCTACCT GGGCAGCCTC AAGCCCGGCG TGCAGGGTAA GAAGGTCCGC 
AAGCCCAGCA GCAAAGGCCT GGCCTGTGGG AGCAAGGAGG CCAAGGACCT CAAGGCGCGG 
AGGAAGAAGT CCCAGGACGG CAAGGGCTGC CTGCTGGACA GCTCCGGCAT GCTCTCACCC 
GTGGACTCCC TGGAGTCGCC CCACGGCTAC CTGTCGGACG TGGCCTCGCC GCCACTGCTG 
CCCTCTCCGT TCCAGCAGTC TCCGTCCGTG CCCCTCAACC ACCTGCCCGG TATGCCCGAC 
ACCCACCTGG GCATCGGGCA CCTGAACGTG GCGGCCAAGC CAGAGATGGC AACACTGGGT 
GGGGGCGGCC GGCTGGCCTT CGAGGCCGGC CCCCCGCGTC TCTCCCACCT GCCCGTGGCC 
TCCAGCACCA GCACCATCCT GGGCTGCAGC AGTGGAGGAG CCATGAATTT CACCGTGGGT 
GGGTCCACCA GTTTGAATGG TCAATGCGAG TGGCTGTCCC GGCTGCAGAG CGGCATGGTG 
CCGAACCAAT ACAACCCTCT GCGGGGGAGC GTGGCACCAG GCCCCCTAAG CACGCAGGCC 
CCCTCCCTGC AGCATGGCAT GGTGGGCCCG CTGCACAGTA GCCTTGCTGC CAGCGCCCTG 
TCCCAGATGA TGAGCTACCA GGGCCTGCCC AGTACCCGGC TGGCCACCCA GCCTCACCTG 
GTGCAGACCC AGCAGGTGCA GCCGCAAAAC TTACAGATGC AGCAGCAGAA CCTGCAGCCA 
CCAAACATCC AGCAGCAGCA AAATCTGCAG CCGCCACCGC CACCACCACA GCCGCACCTT 
GGCGTGAGCT CAGCAGCCAG CGGCCACCTG GGCCGGAGCT TCCTGGGCGG AGAGCCGAGC 
CAGGCAGACG TGCAGCCACT AGGCCCCAGC AGCCTGGCGG TGCACACCAT TCTACCCCAG 
GAGAGCCCTG CTCTGCCCAC GTCGCTGCCG TCCTCGCTGG TCCCACCCGT GACCGCGGCC 
CAGTTCCTGA CACCCCCCTC ACAGCACAGC TACTCCTCCT CGCCTGTGGA CAACACACCC 
AGCCACCAAC TGCAGGTGCC TGAGCACCCC TTCCTCACCC CGTCCCCCGA GTCCCCTGAC 
CAGTGGTCCA GCTCGTCCCC GCATTCCAAC GTCTCCGACT GGTCTGAGGG CGTCTCCAGC 
CCGCCCACCA GCATGCAGTC CCAGATCGCC CGCATTCCGG AGGCCTTCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOTCH1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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