HECTD1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECTD1
Entrez Gene ID	103228783
Full Name	HECT domain E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007986397.1	XP_007984588.1	E3 ubiquitin-protein ligase HECTD1 isoform X2
XM_007986398.1	XP_007984589.1	E3 ubiquitin-protein ligase HECTD1 isoform X3
XM_007986399.1	XP_007984590.1	E3 ubiquitin-protein ligase HECTD1 isoform X4
XM_007986400.1	XP_007984591.1	E3 ubiquitin-protein ligase HECTD1 isoform X5
XM_007986394.1	XP_007984585.1	E3 ubiquitin-protein ligase HECTD1 isoform X1
XM_007986396.1	XP_007984587.1	E3 ubiquitin-protein ligase HECTD1 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECTD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh119294	XM_007986396.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh119294	XM_007986394.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh119295	XM_007986397.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh119296	XM_007986398.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh119297	XM_007986399.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh119298	XM_007986400.1 Latest version!	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECTD1 ( XM_007986394.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984585.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh119294
Clone ID Related Accession (Same CDS sequence)	XM_007986394.1 , XM_007986396.1
Accession Version	XM_007986394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCTG CTTTCCTGCG TCACTGGAAA TTAACCGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA 
AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGACGTC 
CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG 
GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCCTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACTGTGGA ACGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT 
GGAGAGTTTA GAGTTGGTCG TCTCAAGCAT GAGAGAGTAA AAGTTCCACG TGGCGAATCA 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAGTTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG 
GTGGCAGCGG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA 
GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTACGTG 
CAGAGGTCAT GTGGACTGTT CACGGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC GGCTTGGTTT 
AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA 
AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTCA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA 
CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA GTTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAAGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG 
TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG 
CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984585.1
CDS	317..8149
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986394.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986394.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCTG CTTTCCTGCG TCACTGGAAA TTAACCGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA 
AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGACGTC 
CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG 
GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCCTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACTGTGGA ACGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT 
GGAGAGTTTA GAGTTGGTCG TCTCAAGCAT GAGAGAGTAA AAGTTCCACG TGGCGAATCA 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAGTTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG 
GTGGCAGCGG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA 
GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTACGTG 
CAGAGGTCAT GTGGACTGTT CACGGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC GGCTTGGTTT 
AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA 
AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTCA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA 
CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA GTTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAAGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG 
TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG 
CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_007986396.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984587.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

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CloneID	OCh119294
Clone ID Related Accession (Same CDS sequence)	XM_007986394.1 , XM_007986396.1
Accession Version	XM_007986396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCTG CTTTCCTGCG TCACTGGAAA TTAACCGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA 
AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGACGTC 
CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG 
GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCCTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACTGTGGA ACGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT 
GGAGAGTTTA GAGTTGGTCG TCTCAAGCAT GAGAGAGTAA AAGTTCCACG TGGCGAATCA 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAGTTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG 
GTGGCAGCGG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA 
GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTACGTG 
CAGAGGTCAT GTGGACTGTT CACGGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC GGCTTGGTTT 
AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA 
AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTCA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA 
CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA GTTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAAGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG 
TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG 
CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984587.1
CDS	692..8524
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986396.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986396.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAG 
CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT 
GCAGACGCTG CTTTCCTGCG TCACTGGAAA TTAACCGGCA CTAATAAAAG TATTAGGAAA 
AACAGAAATT GTTCTCAGCT CATAGCTGCA TATAAGGATT TTTGTGAGCA TGGAACAAAG 
TCTGGGTTAA ACCAGGGGGC CATTTCTACT CTTCAAAGTA GTGATATTCT TAATTTAACA 
AAAGAACAAC CTCAGGCCAA AGCAGGCAAT GGACAGAACT CTTGTGGAGT AGAAGACGTC 
CTTCAGCTTC TGCGTATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG AATATCCCAG 
GAAGATGGTG ATGAACAGCC TCAGTTTACT TTTCCACCAG ATGAATTCAC TAGCAAAAAA 
ATTACAACAA AAATATTACA GCAGATTGAG GAACCATTGG CATTGGCAAG TGGGGCTCTG 
CCAGACTGGT GTGAACAATT AACCAGCAAA TGTCCTTTTC TAATACCATT TGAAACTAGA 
CAGCTTTATT TCACATGTAC AGCATTTGGT GCCTCAAGAG CAATAGTGTG GTTACAAAAC 
CGACGTGAAG CCACTGTGGA ACGAACGAGA ACCACAAGCA GTGTTAGGCG AGATGACCCT 
GGAGAGTTTA GAGTTGGTCG TCTCAAGCAT GAGAGAGTAA AAGTTCCACG TGGCGAATCA 
CTGATGGAAT GGGCTGAGAA TGTCATGCAA ATACATGCAG ATCGGAAATC AGTTCTTGAG 
GTTGAGTTTT TAGGAGAAGA AGGAACTGGC TTGGGACCCA CATTAGAGTT TTATGCTCTG 
GTGGCAGCGG AATTCCAGAG AACTGACTTG GGAGCTTGGC TTTGTGATGA TAATTTTCCA 
GATGATGAAT CTCGTCACGT TGATCTTGGA GGTGGATTGA AACCTCCTGG ATATTACGTG 
CAGAGGTCAT GTGGACTGTT CACGGCACCA TTTCCACAGG ATAGTGATGA GCTTGAAAGG 
ATCACAAAAC TGTTTCATTT CCTTGGAATT TTCTTGGCCA AATGCATTCA AGACAATAGA 
CTTGTGGACT TACCTATTTC TAAACCTTTT TTTAAACTTA TGTGTATGGG TGACATTAAA 
AGCAATATGA GTAAACTGAT TTATGAGTCA CGAGGTGATA GAGACTTACA CTGTACTGAA 
AGTCAGTCTG AAGCTTCTAC AGAAGAAGGT CATGATTCAC TCTCAGTAGG AAGCTTTGAA 
GAGGATTCAA AATCAGAATT TATTCTTGAT CCCCCTAAAC CAAAACCCCC GGCTTGGTTT 
AATGGAATTT TGACTTGGGA AGACTTTGAA TTAGTAAACC CACACAGAGC CAGATTTTTA 
AAAGAAATTA AAGACCTTGC TATCAAGAGG CGCCAAATTT TAAGCAACAA AGGTCTTTCT 
GAAGATGAGA AGAACACAAA ATTACAGGAA CTAGTGCTGA AGAATCCATC AGGTTCTGGG 
CCTCCACTTA GCATAGAGGA TTTAGGTTTA AATTTCCAGT TTTGCCCTTC CTCAAGAATA 
TATGGTTTCA CAGCTGTGGA TCTCAAGCCA AGTGGTGAAG ATGAGATGAT AACAATGGAT 
AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACAGG TATTCAGAAA 
CAAATGGAAG CCTTTAGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA GTTAAGTTCC 
TTCAGCCATG AAGAAGTCCA AATGATTCTT TGTGGAAACC AGTCACCATC CTGGGCAGCA 
GAAGATATTA TCAATTACAC TGAACCTAAG CTGGGTTATA CACGTGACAG CCCTGGTTTC 
CTGAGGTTTG TGAGGGTTTT ATGTGGCATG TCTTCTGATG AAAGGAAAGC ATTCTTGCAG 
TTTACCACTG GTTGTTCAAC TCTACCCCCA GGTGGACTGG CTAACCTGCA TCCCAGGCTC 
ACGGTTGTAC GCAAGGTTGA TGCTACTGAT GCAAGCTATC CATCAGTCAA TACATGTGTG 
CATTACCTTA AGTTGCCTGA ATATTCTTCC GAGGAGATCA TGAGAGAGCG CCTGCTAGCT 
GCTACAATGG AGAAAGGCTT TCATCTCAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_007986397.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984588.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

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CloneID	OCh119295
Clone ID Related Accession (Same CDS sequence)	XM_007986397.1
Accession Version	XM_007986397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGA TTCTGAGACG CCCATCCCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTA 
ACGCATCATG CTGTTACCTC GCAACTACCA CAGGTTCCTG CTGGAGCAGG GAGCCGACCT 
ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT 
GATGATTATG TGCTAAAGAG ACAATTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT 
GGTCGTACTA ATGTCCAACA GACAACTGAT CTAGAAATAC CACCCCCAGG GACACCTCAT 
TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCCCT CACTTTGAAA 
GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC 
ATCTTTTACT ATGTACAAAA ATTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT 
AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT 
GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC 
CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC 
GCTGCTTTCC TGCGTCACTG GAAATTAACC GGCACTAATA AAAGTATTAG GAAAAACAGA 
AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG 
TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA 
CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA CGTCCTTCAG 
CTTCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT 
GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA 
ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCATTGG CAAGTGGGGC TCTGCCAGAC 
TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT 
TATTTCACAT GTACAGCATT TGGTGCCTCA AGAGCAATAG TGTGGTTACA AAACCGACGT 
GAAGCCACTG TGGAACGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG 
TTTAGAGTTG GTCGTCTCAA GCATGAGAGA GTAAAAGTTC CACGTGGCGA ATCACTGATG 
GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAG 
TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA 
GCGGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT 
GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA CGTGCAGAGG 
TCATGTGGAC TGTTCACGGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACA 
AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG 
GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT 
ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG 
TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCAG TAGGAAGCTT TGAAGAGGAT 
TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCGGCTTG GTTTAATGGA 
ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA 
ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT 
GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA 
CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT 
TTCACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA 
GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CAGGTATTCA GAAACAAATG 
GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAGTTAAG TTCCTTCAGC 
CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAAGAT 
ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG 
TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC 
ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT 
GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC 
CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA 
ATGGAGAAAG GCTTTCATCT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984588.1
CDS	317..8143
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986397.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986397.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGA TTCTGAGACG CCCATCCCTG CAGCGTCGAG CTGGCTCCCG CTCTGATGTA 
ACGCATCATG CTGTTACCTC GCAACTACCA CAGGTTCCTG CTGGAGCAGG GAGCCGACCT 
ATTGGGGAGC AGGAAGAAGA AGAGTACGAA ACTAAAGGAG GACGCCGGAG AACATGGGAT 
GATGATTATG TGCTAAAGAG ACAATTTTCT GCATTGGTTC CTGCTTTTGA TCCTAGACCT 
GGTCGTACTA ATGTCCAACA GACAACTGAT CTAGAAATAC CACCCCCAGG GACACCTCAT 
TCAGAGCTCT TGGAAGAAGT CGAATGTACT CCGTCACCTC GATTAGCCCT CACTTTGAAA 
GTAACAGGTC TTGGAACGAC TCGTGAAGTT GAATTACCAC TCACCAATTT CAGATCAACC 
ATCTTTTACT ATGTACAAAA ATTGCTTCAG TTGTCCTGTA ATGGCAATGT GAAATCAGAT 
AAACTTAGGC GTATTTGGGA GCCCACATAC ACAATCATGT ACAGAGAAAT GAAGGATTCT 
GATAAAGAAA AGGAAAATGG AAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAGCAGTAC 
CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCAGAC 
GCTGCTTTCC TGCGTCACTG GAAATTAACC GGCACTAATA AAAGTATTAG GAAAAACAGA 
AATTGTTCTC AGCTCATAGC TGCATATAAG GATTTTTGTG AGCATGGAAC AAAGTCTGGG 
TTAAACCAGG GGGCCATTTC TACTCTTCAA AGTAGTGATA TTCTTAATTT AACAAAAGAA 
CAACCTCAGG CCAAAGCAGG CAATGGACAG AACTCTTGTG GAGTAGAAGA CGTCCTTCAG 
CTTCTGCGTA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGAATATC CCAGGAAGAT 
GGTGATGAAC AGCCTCAGTT TACTTTTCCA CCAGATGAAT TCACTAGCAA AAAAATTACA 
ACAAAAATAT TACAGCAGAT TGAGGAACCA TTGGCATTGG CAAGTGGGGC TCTGCCAGAC 
TGGTGTGAAC AATTAACCAG CAAATGTCCT TTTCTAATAC CATTTGAAAC TAGACAGCTT 
TATTTCACAT GTACAGCATT TGGTGCCTCA AGAGCAATAG TGTGGTTACA AAACCGACGT 
GAAGCCACTG TGGAACGAAC GAGAACCACA AGCAGTGTTA GGCGAGATGA CCCTGGAGAG 
TTTAGAGTTG GTCGTCTCAA GCATGAGAGA GTAAAAGTTC CACGTGGCGA ATCACTGATG 
GAATGGGCTG AGAATGTCAT GCAAATACAT GCAGATCGGA AATCAGTTCT TGAGGTTGAG 
TTTTTAGGAG AAGAAGGAAC TGGCTTGGGA CCCACATTAG AGTTTTATGC TCTGGTGGCA 
GCGGAATTCC AGAGAACTGA CTTGGGAGCT TGGCTTTGTG ATGATAATTT TCCAGATGAT 
GAATCTCGTC ACGTTGATCT TGGAGGTGGA TTGAAACCTC CTGGATATTA CGTGCAGAGG 
TCATGTGGAC TGTTCACGGC ACCATTTCCA CAGGATAGTG ATGAGCTTGA AAGGATCACA 
AAACTGTTTC ATTTCCTTGG AATTTTCTTG GCCAAATGCA TTCAAGACAA TAGACTTGTG 
GACTTACCTA TTTCTAAACC TTTTTTTAAA CTTATGTGTA TGGGTGACAT TAAAAGCAAT 
ATGAGTAAAC TGATTTATGA GTCACGAGGT GATAGAGACT TACACTGTAC TGAAAGTCAG 
TCTGAAGCTT CTACAGAAGA AGGTCATGAT TCACTCTCAG TAGGAAGCTT TGAAGAGGAT 
TCAAAATCAG AATTTATTCT TGATCCCCCT AAACCAAAAC CCCCGGCTTG GTTTAATGGA 
ATTTTGACTT GGGAAGACTT TGAATTAGTA AACCCACACA GAGCCAGATT TTTAAAAGAA 
ATTAAAGACC TTGCTATCAA GAGGCGCCAA ATTTTAAGCA ACAAAGGTCT TTCTGAAGAT 
GAGAAGAACA CAAAATTACA GGAACTAGTG CTGAAGAATC CATCAGGTTC TGGGCCTCCA 
CTTAGCATAG AGGATTTAGG TTTAAATTTC CAGTTTTGCC CTTCCTCAAG AATATATGGT 
TTCACAGCTG TGGATCTCAA GCCAAGTGGT GAAGATGAGA TGATAACAAT GGATAATGCA 
GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CAGGTATTCA GAAACAAATG 
GAAGCCTTTA GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAGTTAAG TTCCTTCAGC 
CATGAAGAAG TCCAAATGAT TCTTTGTGGA AACCAGTCAC CATCCTGGGC AGCAGAAGAT 
ATTATCAATT ACACTGAACC TAAGCTGGGT TATACACGTG ACAGCCCTGG TTTCCTGAGG 
TTTGTGAGGG TTTTATGTGG CATGTCTTCT GATGAAAGGA AAGCATTCTT GCAGTTTACC 
ACTGGTTGTT CAACTCTACC CCCAGGTGGA CTGGCTAACC TGCATCCCAG GCTCACGGTT 
GTACGCAAGG TTGATGCTAC TGATGCAAGC TATCCATCAG TCAATACATG TGTGCATTAC 
CTTAAGTTGC CTGAATATTC TTCCGAGGAG ATCATGAGAG AGCGCCTGCT AGCTGCTACA 
ATGGAGAAAG GCTTTCATCT CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_007986398.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984589.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

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CloneID	OCh119296
Clone ID Related Accession (Same CDS sequence)	XM_007986398.1
Accession Version	XM_007986398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC 
TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT 
GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA 
TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT 
GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCGTCTTC CAGTGCAAGC 
ACCAGCACCT TAACAGCGGA GACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCCGAT 
AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT 
TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT 
CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT 
ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT 
TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC 
CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT 
TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA 
GTGACTATGT CAACGTCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT 
TTATCAGTTG GTCAGTCATT GAGTAATACT TTAACCACCA GCCTCACATC AACTTCCAGT 
GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT 
GCCAGTACTC TGTTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA 
GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG 
AGACGCCCAT CCCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT 
ACCTCGCAAC TACCACAGGT TCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA 
GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA 
AAGAGACAAT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC 
CAACAGACAA CTGATCTAGA AATACCACCC CCAGGGACAC CTCATTCAGA GCTCTTGGAA 
GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA 
ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA 
CAAAAATTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT 
TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA 
AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA 
TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGT 
CACTGGAAAT TAACCGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC 
ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC 
ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA 
GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGACGTCC TTCAGCTTCT GCGTATTCTG 
TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT 
CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG 
CAGATTGAGG AACCATTGGC ATTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA 
ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA 
GCATTTGGTG CCTCAAGAGC AATAGTGTGG TTACAAAACC GACGTGAAGC CACTGTGGAA 
CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTAG AGTTGGTCGT 
CTCAAGCATG AGAGAGTAAA AGTTCCACGT GGCGAATCAC TGATGGAATG GGCTGAGAAT 
GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAGTTTTT AGGAGAAGAA 
GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCGGA ATTCCAGAGA 
ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT 
GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTACGTGC AGAGGTCATG TGGACTGTTC 
ACGGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACAAAACT GTTTCATTTC 
CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT 
AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT 
TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA 
GAAGAAGGTC ATGATTCACT CTCAGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT 
ATTCTTGATC CCCCTAAACC AAAACCCCCG GCTTGGTTTA ATGGAATTTT GACTTGGGAA 
GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT 
ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA 
TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT 
TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTCAC AGCTGTGGAT 
CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT 
TTGATGTTTG ACTTTTGTAT GCATACAGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT 
GGGTTTAATA AAGTTTTTCC AATGGAGAAG TTAAGTTCCT TCAGCCATGA AGAAGTCCAA 
ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AAGATATTAT CAATTACACT 
GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA 
TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT 
CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT 
GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA 
TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT 
CATCTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984589.1
CDS	317..8008
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986398.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986398.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCACAACG CAATCATGGA GCAGCTTGGT GAAAAACAAC 
TGTCCAGACA AGACATCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT 
GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA 
TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT 
GTTCTTTCAT CTGCTGAAAA CGTCCCTCAA ACAGAAGTAG GGTCGTCTTC CAGTGCAAGC 
ACCAGCACCT TAACAGCGGA GACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCCGAT 
AGTTCTGTTC GTACTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT CAGTGTTAGT 
TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAAG AAGCAGCTTC ACAACGACCT 
CTTAGCTCTT CAGCAAGTAA CAGACTGTCA GTGAGTTCTT TGTTGGCTGC TGGGGCCCCT 
ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGT 
TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CGAACAACAT GAATCTAAGC 
CGAAGCAGCA GTGATAACAA CACTAATACT TTGGGGAGGA ATGTGATGAG CACAGCAACT 
TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCAACA 
GTGACTATGT CAACGTCCAG TGTTACTAGC AGCAGCAATG TAGCTACAGC AACAACAGTT 
TTATCAGTTG GTCAGTCATT GAGTAATACT TTAACCACCA GCCTCACATC AACTTCCAGT 
GAGAGTGACA CAGGTCAGGA AGCAGAATAT TCCTTATATG ATTTCCTTGA TAGCTGCCGT 
GCCAGTACTC TGTTGGCTGA GCTCGATGAT GATGAGGACT TACCTGAGCC AGATGAAGAA 
GATGATGAGA ATGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG 
AGACGCCCAT CCCTGCAGCG TCGAGCTGGC TCCCGCTCTG ATGTAACGCA TCATGCTGTT 
ACCTCGCAAC TACCACAGGT TCCTGCTGGA GCAGGGAGCC GACCTATTGG GGAGCAGGAA 
GAAGAAGAGT ACGAAACTAA AGGAGGACGC CGGAGAACAT GGGATGATGA TTATGTGCTA 
AAGAGACAAT TTTCTGCATT GGTTCCTGCT TTTGATCCTA GACCTGGTCG TACTAATGTC 
CAACAGACAA CTGATCTAGA AATACCACCC CCAGGGACAC CTCATTCAGA GCTCTTGGAA 
GAAGTCGAAT GTACTCCGTC ACCTCGATTA GCCCTCACTT TGAAAGTAAC AGGTCTTGGA 
ACGACTCGTG AAGTTGAATT ACCACTCACC AATTTCAGAT CAACCATCTT TTACTATGTA 
CAAAAATTGC TTCAGTTGTC CTGTAATGGC AATGTGAAAT CAGATAAACT TAGGCGTATT 
TGGGAGCCCA CATACACAAT CATGTACAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA 
AATGGAAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAGC AGTACCTTGG CACTGATGAA 
TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CAGACGCTGC TTTCCTGCGT 
CACTGGAAAT TAACCGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTC 
ATAGCTGCAT ATAAGGATTT TTGTGAGCAT GGAACAAAGT CTGGGTTAAA CCAGGGGGCC 
ATTTCTACTC TTCAAAGTAG TGATATTCTT AATTTAACAA AAGAACAACC TCAGGCCAAA 
GCAGGCAATG GACAGAACTC TTGTGGAGTA GAAGACGTCC TTCAGCTTCT GCGTATTCTG 
TATATAGTTG CAAGTGACCC TTATTCAAGA ATATCCCAGG AAGATGGTGA TGAACAGCCT 
CAGTTTACTT TTCCACCAGA TGAATTCACT AGCAAAAAAA TTACAACAAA AATATTACAG 
CAGATTGAGG AACCATTGGC ATTGGCAAGT GGGGCTCTGC CAGACTGGTG TGAACAATTA 
ACCAGCAAAT GTCCTTTTCT AATACCATTT GAAACTAGAC AGCTTTATTT CACATGTACA 
GCATTTGGTG CCTCAAGAGC AATAGTGTGG TTACAAAACC GACGTGAAGC CACTGTGGAA 
CGAACGAGAA CCACAAGCAG TGTTAGGCGA GATGACCCTG GAGAGTTTAG AGTTGGTCGT 
CTCAAGCATG AGAGAGTAAA AGTTCCACGT GGCGAATCAC TGATGGAATG GGCTGAGAAT 
GTCATGCAAA TACATGCAGA TCGGAAATCA GTTCTTGAGG TTGAGTTTTT AGGAGAAGAA 
GGAACTGGCT TGGGACCCAC ATTAGAGTTT TATGCTCTGG TGGCAGCGGA ATTCCAGAGA 
ACTGACTTGG GAGCTTGGCT TTGTGATGAT AATTTTCCAG ATGATGAATC TCGTCACGTT 
GATCTTGGAG GTGGATTGAA ACCTCCTGGA TATTACGTGC AGAGGTCATG TGGACTGTTC 
ACGGCACCAT TTCCACAGGA TAGTGATGAG CTTGAAAGGA TCACAAAACT GTTTCATTTC 
CTTGGAATTT TCTTGGCCAA ATGCATTCAA GACAATAGAC TTGTGGACTT ACCTATTTCT 
AAACCTTTTT TTAAACTTAT GTGTATGGGT GACATTAAAA GCAATATGAG TAAACTGATT 
TATGAGTCAC GAGGTGATAG AGACTTACAC TGTACTGAAA GTCAGTCTGA AGCTTCTACA 
GAAGAAGGTC ATGATTCACT CTCAGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT 
ATTCTTGATC CCCCTAAACC AAAACCCCCG GCTTGGTTTA ATGGAATTTT GACTTGGGAA 
GACTTTGAAT TAGTAAACCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT 
ATCAAGAGGC GCCAAATTTT AAGCAACAAA GGTCTTTCTG AAGATGAGAA GAACACAAAA 
TTACAGGAAC TAGTGCTGAA GAATCCATCA GGTTCTGGGC CTCCACTTAG CATAGAGGAT 
TTAGGTTTAA ATTTCCAGTT TTGCCCTTCC TCAAGAATAT ATGGTTTCAC AGCTGTGGAT 
CTCAAGCCAA GTGGTGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT 
TTGATGTTTG ACTTTTGTAT GCATACAGGT ATTCAGAAAC AAATGGAAGC CTTTAGAGAT 
GGGTTTAATA AAGTTTTTCC AATGGAGAAG TTAAGTTCCT TCAGCCATGA AGAAGTCCAA 
ATGATTCTTT GTGGAAACCA GTCACCATCC TGGGCAGCAG AAGATATTAT CAATTACACT 
GAACCTAAGC TGGGTTATAC ACGTGACAGC CCTGGTTTCC TGAGGTTTGT GAGGGTTTTA 
TGTGGCATGT CTTCTGATGA AAGGAAAGCA TTCTTGCAGT TTACCACTGG TTGTTCAACT 
CTACCCCCAG GTGGACTGGC TAACCTGCAT CCCAGGCTCA CGGTTGTACG CAAGGTTGAT 
GCTACTGATG CAAGCTATCC ATCAGTCAAT ACATGTGTGC ATTACCTTAA GTTGCCTGAA 
TATTCTTCCG AGGAGATCAT GAGAGAGCGC CTGCTAGCTG CTACAATGGA GAAAGGCTTT 
CATCTCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_007986399.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984590.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

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CloneID	OCh119297
Clone ID Related Accession (Same CDS sequence)	XM_007986399.1
Accession Version	XM_007986399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT 
GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA 
GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGACGTCCTT 
CAGCTTCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA 
GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT 
ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAT TGGCAAGTGG GGCTCTGCCA 
GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG 
CTTTATTTCA CATGTACAGC ATTTGGTGCC TCAAGAGCAA TAGTGTGGTT ACAAAACCGA 
CGTGAAGCCA CTGTGGAACG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA 
GAGTTTAGAG TTGGTCGTCT CAAGCATGAG AGAGTAAAAG TTCCACGTGG CGAATCACTG 
ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT 
GAGTTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG 
GCAGCGGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT 
GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTACGTGCAG 
AGGTCATGTG GACTGTTCAC GGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC 
ACAAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT 
GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC 
AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT 
CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CAGTAGGAAG CTTTGAAGAG 
GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCGGC TTGGTTTAAT 
GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA 
GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA 
GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT 
CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT 
GGTTTCACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT 
GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACAGGTAT TCAGAAACAA 
ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAGTT AAGTTCCTTC 
AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAA 
GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG 
AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT 
ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG 
GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT 
TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACAATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984590.1
CDS	317..7966
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNGWIDVTWDAGGSNSYRMGAEGKFDLKLAPGYDPDTVASPKPVSSTVSGTTQSWSSLVKNNCPDKTSAAAGSSSRKGSSSSVCSVASSSDISLGSTKTERRSEIVMEHSIVSGADVHEPIVVLSSAENVPQTEVGSSSSASTSTLTAETGSENAERKLGPDSSVRTPGESSAISMGIVSVSSPDVSSVSELTNKEAASQRPLSSSASNRLSVSSLLAAGAPMSSSASVPNLSSRETSSLESFVRRVANIARTNATNNMNLSRSSSDNNTNTLGRNVMSTATSPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986399.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986399.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC 
TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT 
GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAATCATGG 
AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACATCTG CTGCTGCAGG CTCCTCAAGT 
AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT 
TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT 
GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTCCCTCA AACAGAAGTA 
GGGTCGTCTT CCAGTGCAAG CACCAGCACC TTAACAGCGG AGACGGGAAG TGAAAATGCT 
GAAAGGAAGT TAGGCCCCGA TAGTTCTGTT CGTACTCCTG GGGAGTCTAG TGCAATATCC 
ATGGGAATTG TCAGTGTTAG TTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAA 
GAAGCAGCTT CACAACGACC TCTTAGCTCT TCAGCAAGTA ACAGACTGTC AGTGAGTTCT 
TTGTTGGCTG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA 
GAAACATCTA GCTTGGAGAG TTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC 
ACGAACAACA TGAATCTAAG CCGAAGCAGC AGTGATAACA ACACTAATAC TTTGGGGAGG 
AATGTGATGA GCACAGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC 
ACACCTGGTA CTACATCAAC AGTGACTATG TCAACGTCCA GTGTTACTAG CAGCAGCAAT 
GTAGCTACAG CAACAACAGT TTTATCAGTT GGTCAGTCAT TGAGTAATAC TTTAACCACC 
AGCCTCACAT CAACTTCCAG TGAGAGTGAC ACAGGTCAGG AAGCAGAATA TTCCTTATAT 
GATTTCCTTG ATAGCTGCCG TGCCAGTACT CTGTTGGCTG AGCTCGATGA TGATGAGGAC 
TTACCTGAGC CAGATGAAGA AGATGATGAG AATGAAGATG ACAATCAGGA GGACCAAGAA 
TACGAGGAGG TTATGATTCT GAGACGCCCA TCCCTGCAGC GTCGAGCTGG CTCCCGCTCT 
GATGTAACGC ATCATGCTGT TACCTCGCAA CTACCACAGG TTCCTGCTGG AGCAGGGAGC 
CGACCTATTG GGGAGCAGGA AGAAGAAGAG TACGAAACTA AAGGAGGACG CCGGAGAACA 
TGGGATGATG ATTATGTGCT AAAGAGACAA TTTTCTGCAT TGGTTCCTGC TTTTGATCCT 
AGACCTGGTC GTACTAATGT CCAACAGACA ACTGATCTAG AAATACCACC CCCAGGGACA 
CCTCATTCAG AGCTCTTGGA AGAAGTCGAA TGTACTCCGT CACCTCGATT AGCCCTCACT 
TTGAAAGTAA CAGGTCTTGG AACGACTCGT GAAGTTGAAT TACCACTCAC CAATTTCAGA 
TCAACCATCT TTTACTATGT ACAAAAATTG CTTCAGTTGT CCTGTAATGG CAATGTGAAA 
TCAGATAAAC TTAGGCGTAT TTGGGAGCCC ACATACACAA TCATGTACAG AGAAATGAAG 
GATTCTGATA AAGAAAAGGA AAATGGAAAA ATGGATTTTT GTGAGCATGG AACAAAGTCT 
GGGTTAAACC AGGGGGCCAT TTCTACTCTT CAAAGTAGTG ATATTCTTAA TTTAACAAAA 
GAACAACCTC AGGCCAAAGC AGGCAATGGA CAGAACTCTT GTGGAGTAGA AGACGTCCTT 
CAGCTTCTGC GTATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGAAT ATCCCAGGAA 
GATGGTGATG AACAGCCTCA GTTTACTTTT CCACCAGATG AATTCACTAG CAAAAAAATT 
ACAACAAAAA TATTACAGCA GATTGAGGAA CCATTGGCAT TGGCAAGTGG GGCTCTGCCA 
GACTGGTGTG AACAATTAAC CAGCAAATGT CCTTTTCTAA TACCATTTGA AACTAGACAG 
CTTTATTTCA CATGTACAGC ATTTGGTGCC TCAAGAGCAA TAGTGTGGTT ACAAAACCGA 
CGTGAAGCCA CTGTGGAACG AACGAGAACC ACAAGCAGTG TTAGGCGAGA TGACCCTGGA 
GAGTTTAGAG TTGGTCGTCT CAAGCATGAG AGAGTAAAAG TTCCACGTGG CGAATCACTG 
ATGGAATGGG CTGAGAATGT CATGCAAATA CATGCAGATC GGAAATCAGT TCTTGAGGTT 
GAGTTTTTAG GAGAAGAAGG AACTGGCTTG GGACCCACAT TAGAGTTTTA TGCTCTGGTG 
GCAGCGGAAT TCCAGAGAAC TGACTTGGGA GCTTGGCTTT GTGATGATAA TTTTCCAGAT 
GATGAATCTC GTCACGTTGA TCTTGGAGGT GGATTGAAAC CTCCTGGATA TTACGTGCAG 
AGGTCATGTG GACTGTTCAC GGCACCATTT CCACAGGATA GTGATGAGCT TGAAAGGATC 
ACAAAACTGT TTCATTTCCT TGGAATTTTC TTGGCCAAAT GCATTCAAGA CAATAGACTT 
GTGGACTTAC CTATTTCTAA ACCTTTTTTT AAACTTATGT GTATGGGTGA CATTAAAAGC 
AATATGAGTA AACTGATTTA TGAGTCACGA GGTGATAGAG ACTTACACTG TACTGAAAGT 
CAGTCTGAAG CTTCTACAGA AGAAGGTCAT GATTCACTCT CAGTAGGAAG CTTTGAAGAG 
GATTCAAAAT CAGAATTTAT TCTTGATCCC CCTAAACCAA AACCCCCGGC TTGGTTTAAT 
GGAATTTTGA CTTGGGAAGA CTTTGAATTA GTAAACCCAC ACAGAGCCAG ATTTTTAAAA 
GAAATTAAAG ACCTTGCTAT CAAGAGGCGC CAAATTTTAA GCAACAAAGG TCTTTCTGAA 
GATGAGAAGA ACACAAAATT ACAGGAACTA GTGCTGAAGA ATCCATCAGG TTCTGGGCCT 
CCACTTAGCA TAGAGGATTT AGGTTTAAAT TTCCAGTTTT GCCCTTCCTC AAGAATATAT 
GGTTTCACAG CTGTGGATCT CAAGCCAAGT GGTGAAGATG AGATGATAAC AATGGATAAT 
GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACAGGTAT TCAGAAACAA 
ATGGAAGCCT TTAGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAGTT AAGTTCCTTC 
AGCCATGAAG AAGTCCAAAT GATTCTTTGT GGAAACCAGT CACCATCCTG GGCAGCAGAA 
GATATTATCA ATTACACTGA ACCTAAGCTG GGTTATACAC GTGACAGCCC TGGTTTCCTG 
AGGTTTGTGA GGGTTTTATG TGGCATGTCT TCTGATGAAA GGAAAGCATT CTTGCAGTTT 
ACCACTGGTT GTTCAACTCT ACCCCCAGGT GGACTGGCTA ACCTGCATCC CAGGCTCACG 
GTTGTACGCA AGGTTGATGC TACTGATGCA AGCTATCCAT CAGTCAATAC ATGTGTGCAT 
TACCTTAAGT TGCCTGAATA TTCTTCCGAG GAGATCATGA GAGAGCGCCT GCTAGCTGCT 
ACAATGGAGA AAGGCTTTCA TCTCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECTD1 ( XM_007986400.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007984591.1 Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

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CloneID	OCh119298
Clone ID Related Accession (Same CDS sequence)	XM_007986400.1
Accession Version	XM_007986400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	E3 ubiquitin-protein ligase HECTD1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735274.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG 
ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACGT CCAGTGTTAC TAGCAGCAGC 
AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CATTGAGTAA TACTTTAACC 
ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA 
TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTGG CTGAGCTCGA TGATGATGAG 
GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA 
GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AGCGTCGAGC TGGCTCCCGC 
TCTGATGTAA CGCATCATGC TGTTACCTCG CAACTACCAC AGGTTCCTGC TGGAGCAGGG 
AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA 
ACATGGGATG ATGATTATGT GCTAAAGAGA CAATTTTCTG CATTGGTTCC TGCTTTTGAT 
CCTAGACCTG GTCGTACTAA TGTCCAACAG ACAACTGATC TAGAAATACC ACCCCCAGGG 
ACACCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCCCTC 
ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC 
AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAGT TGTCCTGTAA TGGCAATGTG 
AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG 
AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG 
GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG 
AATGCAGACG CTGCTTTCCT GCGTCACTGG AAATTAACCG GCACTAATAA AAGTATTAGG 
AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA 
AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA 
ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAC 
GTCCTTCAGC TTCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC 
CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA 
AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCATTGGC AAGTGGGGCT 
CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT 
AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCCTCAA GAGCAATAGT GTGGTTACAA 
AACCGACGTG AAGCCACTGT GGAACGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC 
CCTGGAGAGT TTAGAGTTGG TCGTCTCAAG CATGAGAGAG TAAAAGTTCC ACGTGGCGAA 
TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT 
GAGGTTGAGT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT 
CTGGTGGCAG CGGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT 
CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAC 
GTGCAGAGGT CATGTGGACT GTTCACGGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA 
AGGATCACAA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT 
AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT 
AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT 
GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCAGT AGGAAGCTTT 
GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCGGCTTGG 
TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT 
TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT 
TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT 
GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA 
ATATATGGTT TCACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG 
GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC AGGTATTCAG 
AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAGTTAAGT 
TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA 
GCAGAAGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT 
TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG 
CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG 
CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT 
GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA 
GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007984591.1
CDS	316..7311
Translation	MADVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVELNNRTSRDLAEQCVKVLELICTRESGAVFEAGGLNCVLTFIRDSGHLVHKDTLHSAMAVVSRLCGKMEPQDSSLEICVESLSSLLKHEDHQVSDGALRCFASLADRFTRRGVDPAPLAKHGLTEELLSRMAAAGGTVSGPSSACKPGRSTTGAPSTTADSKLSNQVSTIVSLLSTLCRGSPVVTHDLLRSELPDSIESALQGDERCVLDTMRLVDLLLVLLFEGRKALPKSSAGSTGRIPGLRRLDSSGERSHRQLIDCIRSKDTDALIDAIDTGAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRSSSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARERGHSEVVAILQSPGDWMCPVNKGDDKKKKDTNKDEEECNEPKGDPEMAPIYLKRLLPVFAQTFQQTMLPSIRKASLALIRKMIHFCSEALLKEVCDSDVGHNLPTILVEITATVLDQEDDDDGHLLALQIIRDLVDKGGDIFLDQLARLGVISKVSTLAGPSSDDENEEESKPEKEDEPQEDAKELQQGKPYHWRDWSIIRGRDCLYIWSDAAALELSNGSNGWFRFILDGKLATMYSSGSPEGGSDSSESRSEFLEKLQRARGQVKPSTSSQPILSAPGPTKLTVGNWSLTCLKEGEIAIHNSDGQQATILKEDLPGFVFESNRGTKHSFTAETSLGSEFVTGWTGKRGRKLKSKLEKTKQKVRTMARDLYDDHFKAVESMPRGVVVTLRNIATQLESSWELHTNRQCIESENTWRDLMKTALENLIVLLKDENTISPYEMCSSGLVQALLTVLNNSMDLDMKQDCSQLVERINVFKTAFSENEDDESRPAVALIRKLIAVLESIERLPLHLYDTPGSTYNLQILTRRLRFRLERAPGETALIDRTGRMLKMEPLATVESLEQYLLKMVAKQWYDFDRSSFVFVRKLREGQNFIFRHQHDFDENGIIYWIGTNAKTAYEWVNPAAYGLVVVTSSEGRNLPYGRLEDILSRDNSALNCHSNDDKNAWFAIDLGLWVIPSAYTLRHARGYGRSALRNWVFQVSKDGQNWTSLYTHVDDCSLNEPGSTATWPLDPPKDEKQGWRHVRIKQMGKNASGQTHYLSLSGFELYGTVNGVCEDQLGKAAKEAEANLRRQRRLVRSQVLKYMVPGARVIRGLDWKWRDQDGSPQGEGTVTGELHNASPLMGAQSFPNLTTPGTTSTVTMSTSSVTSSSNVATATTVLSVGQSLSNTLTTSLTSTSSESDTGQEAEYSLYDFLDSCRASTLLAELDDDEDLPEPDEEDDENEDDNQEDQEYEEVMILRRPSLQRRAGSRSDVTHHAVTSQLPQVPAGAGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQPQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN

Target ORF information:

RefSeq Version	XM_007986400.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus HECT domain containing E3 ubiquitin protein ligase 1 (HECTD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007986400.1

ORF Insert Sequence:

ATGGCAGATG TGGACCCAGA TACATTGCTG GAATGGCTAC AGATGGGACA GGGAGATGAA 
AGGGACATGC AACTAATAGC CCTTGAACAG CTATGCATGC TGCTTTTGAT GTCTGACAAC 
GTGGATCGTT GTTTTGAAAC ATGTCCTCCT CGCACTTTCT TACCAGCCCT TTGCAAAATT 
TTTCTTGATG AAAGTGCTCC AGACAATGTA TTAGAGGTGA CAGCCCGTGC CATAACATAC 
TACTTGGATG TATCTGCAGA ATGTACCCGA AGGATTGTTG GGGTTGATGG AGCTATAAAA 
GCACTTTGTA ATCGTTTGGT TGTAGTTGAA CTTAACAACA GGACTAGCAG AGACTTAGCT 
GAACAATGTG TAAAGGTATT AGAACTGATA TGTACTCGTG AGTCAGGAGC AGTCTTTGAG 
GCTGGTGGTT TGAATTGTGT GCTTACCTTC ATTCGTGACA GTGGACATCT AGTTCATAAA 
GACACCTTGC ACTCTGCCAT GGCTGTGGTA TCAAGACTCT GTGGCAAAAT GGAGCCTCAA 
GATTCTTCTT TAGAAATTTG TGTAGAATCT CTGTCTAGTT TATTAAAACA TGAAGATCAT 
CAGGTTTCAG ATGGAGCTCT GCGATGCTTT GCATCACTGG CTGACCGATT TACCCGTCGT 
GGTGTTGACC CAGCTCCATT AGCCAAGCAT GGATTAACTG AGGAGCTGTT ATCGCGAATG 
GCTGCTGCTG GTGGTACTGT ATCAGGACCA TCATCAGCAT GTAAACCAGG TCGCAGCACC 
ACAGGAGCTC CTTCCACCAC TGCAGATTCC AAATTGAGTA ATCAGGTGTC AACAATTGTG 
AGTCTGCTCT CAACACTTTG CAGAGGCTCT CCGGTAGTAA CACATGATCT TTTGAGGTCG 
GAGCTTCCAG ATTCAATTGA AAGTGCACTG CAGGGTGATG AAAGATGTGT GCTTGATACT 
ATGCGTTTGG TTGACCTTCT CTTGGTGCTA TTATTTGAAG GACGAAAAGC TTTGCCAAAG 
TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTCCGGA GATTAGATAG TTCTGGGGAG 
CGCTCACATC GGCAGCTGAT AGATTGTATT CGAAGTAAAG ATACCGATGC ACTTATAGAT 
GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTAGGTCA GACTCTATTA 
AACTGGGCCT CTGCTTTTGG AACTCAGGAA ATGGTAGAAT TTCTTTGTGA GAGAGGTGCA 
GATGTTAATA GAGGTCAAAG GTCATCATCA TTACATTATG CTGCATGTTT TGGAAGACCT 
CAAGTAGCAA AGACTCTGTT ACGACATGGT GCAAATCCAG ATCTAAGAGA TGAAGATGGG 
AAAACTCCAT TAGATAAAGC TCGAGAAAGG GGTCATAGTG AAGTAGTAGC TATTCTTCAG 
TCTCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGAGATG ATAAGAAAAA GAAAGATACA 
AACAAAGATG AAGAAGAATG TAATGAGCCC AAAGGAGATC CGGAAATGGC ACCCATATAC 
TTGAAAAGGT TATTGCCAGT GTTTGCACAA ACATTTCAGC AAACTATGCT GCCTTCAATA 
AGGAAAGCAA GTCTTGCTCT AATTCGAAAA ATGATTCATT TTTGCTCTGA AGCACTGTTA 
AAAGAAGTTT GCGATTCTGA TGTTGGTCAC AATTTGCCTA CAATACTAGT GGAAATCACT 
GCAACTGTCC TGGATCAAGA GGATGATGAT GATGGCCACT TGCTGGCTTT GCAGATCATA 
AGGGATTTAG TAGATAAAGG CGGTGATATA TTTTTGGATC AGCTAGCCAG ACTTGGTGTA 
ATTAGCAAAG TGTCTACGTT GGCAGGTCCT TCCTCTGATG ATGAGAATGA AGAGGAATCA 
AAACCAGAAA AAGAAGATGA ACCACAGGAA GATGCTAAAG AATTGCAGCA AGGTAAACCA 
TATCATTGGA GAGACTGGTC AATCATTAGG GGAAGAGACT GCTTGTATAT TTGGAGTGAT 
GCAGCAGCCT TGGAATTATC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA 
AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCTGACAG TTCAGAAAGC 
CGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCTCGAGGCC AAGTAAAGCC ATCTACTTCA 
AGTCAACCTA TACTGTCAGC ACCAGGACCC ACTAAACTTA CTGTAGGAAA TTGGTCACTG 
ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAATTCAG ATGGTCAGCA AGCTACAATA 
TTGAAAGAAG ATTTACCTGG TTTTGTATTT GAATCTAATA GAGGAACCAA ACATTCATTT 
ACTGCAGAAA CTTCCCTGGG TTCAGAATTT GTGACTGGCT GGACTGGCAA AAGAGGCAGA 
AAACTGAAAT CTAAGTTAGA AAAAACAAAG CAAAAGGTAC GAACTATGGC TCGAGATTTA 
TACGATGACC ACTTTAAAGC TGTTGAAAGC ATGCCTCGTG GAGTAGTGGT AACACTCAGA 
AACATAGCAA CTCAGTTAGA GTCATCTTGG GAGCTTCATA CAAATAGACA ATGTATTGAG 
AGTGAGAACA CTTGGAGAGA TTTAATGAAG ACGGCTTTAG AAAACCTAAT TGTACTTTTG 
AAGGATGAAA ACACAATTTC ACCATATGAA ATGTGTAGCA GTGGCTTGGT ACAGGCACTT 
CTTACTGTGT TAAACAATAG CATGGATTTG GATATGAAAC AAGATTGTAG TCAACTGGTA 
GAAAGAATAA ATGTTTTTAA AACTGCCTTT AGTGAAAATG AAGATGATGA AAGTCGACCA 
GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CTATTGAACG CCTACCTCTC 
CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTTACAAG GAGATTACGA 
TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTGATTG ACAGGACTGG CAGAATGTTG 
AAGATGGAAC CTTTGGCTAC AGTTGAATCT CTGGAACAGT ACCTTTTGAA AATGGTAGCA 
AAACAGTGGT ATGATTTTGA CCGATCTTCA TTTGTTTTTG TTCGAAAATT AAGAGAAGGA 
CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT 
GGAACAAATG CAAAAACTGC TTATGAATGG GTAAATCCGG CTGCCTATGG ACTTGTAGTA 
GTAACGTCAT CAGAAGGAAG AAATCTACCT TATGGCCGCT TAGAAGACAT ACTAAGTCGT 
GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAGA ATGCCTGGTT TGCCATAGAT 
CTGGGTCTCT GGGTGATACC ATCAGCATAT ACACTTCGTC ATGCTCGTGG TTATGGAAGG 
TCTGCACTGA GAAATTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG 
TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC TTGGCCTCTT 
GATCCACCAA AGGATGAGAA ACAAGGGTGG AGACATGTGA GAATTAAACA GATGGGGAAA 
AATGCCAGTG GACAGACACA CTACCTCTCA CTATCTGGAT TTGAACTTTA TGGCACTGTA 
AATGGAGTAT GTGAAGATCA GCTAGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA 
CGGCAGAGAC GTCTAGTACG CTCCCAGGTT CTGAAATACA TGGTTCCAGG AGCTCGTGTT 
ATCAGAGGTT TGGATTGGAA ATGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACT 
GTCACAGGAG AACTGCACAA TGCTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG 
ACCACACCTG GTACTACATC AACAGTGACT ATGTCAACGT CCAGTGTTAC TAGCAGCAGC 
AATGTAGCTA CAGCAACAAC AGTTTTATCA GTTGGTCAGT CATTGAGTAA TACTTTAACC 
ACCAGCCTCA CATCAACTTC CAGTGAGAGT GACACAGGTC AGGAAGCAGA ATATTCCTTA 
TATGATTTCC TTGATAGCTG CCGTGCCAGT ACTCTGTTGG CTGAGCTCGA TGATGATGAG 
GACTTACCTG AGCCAGATGA AGAAGATGAT GAGAATGAAG ATGACAATCA GGAGGACCAA 
GAATACGAGG AGGTTATGAT TCTGAGACGC CCATCCCTGC AGCGTCGAGC TGGCTCCCGC 
TCTGATGTAA CGCATCATGC TGTTACCTCG CAACTACCAC AGGTTCCTGC TGGAGCAGGG 
AGCCGACCTA TTGGGGAGCA GGAAGAAGAA GAGTACGAAA CTAAAGGAGG ACGCCGGAGA 
ACATGGGATG ATGATTATGT GCTAAAGAGA CAATTTTCTG CATTGGTTCC TGCTTTTGAT 
CCTAGACCTG GTCGTACTAA TGTCCAACAG ACAACTGATC TAGAAATACC ACCCCCAGGG 
ACACCTCATT CAGAGCTCTT GGAAGAAGTC GAATGTACTC CGTCACCTCG ATTAGCCCTC 
ACTTTGAAAG TAACAGGTCT TGGAACGACT CGTGAAGTTG AATTACCACT CACCAATTTC 
AGATCAACCA TCTTTTACTA TGTACAAAAA TTGCTTCAGT TGTCCTGTAA TGGCAATGTG 
AAATCAGATA AACTTAGGCG TATTTGGGAG CCCACATACA CAATCATGTA CAGAGAAATG 
AAGGATTCTG ATAAAGAAAA GGAAAATGGA AAAATGGGTT GCTGGTCTAT AGAGCATGTG 
GAGCAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG 
AATGCAGACG CTGCTTTCCT GCGTCACTGG AAATTAACCG GCACTAATAA AAGTATTAGG 
AAAAACAGAA ATTGTTCTCA GCTCATAGCT GCATATAAGG ATTTTTGTGA GCATGGAACA 
AAGTCTGGGT TAAACCAGGG GGCCATTTCT ACTCTTCAAA GTAGTGATAT TCTTAATTTA 
ACAAAAGAAC AACCTCAGGC CAAAGCAGGC AATGGACAGA ACTCTTGTGG AGTAGAAGAC 
GTCCTTCAGC TTCTGCGTAT TCTGTATATA GTTGCAAGTG ACCCTTATTC AAGAATATCC 
CAGGAAGATG GTGATGAACA GCCTCAGTTT ACTTTTCCAC CAGATGAATT CACTAGCAAA 
AAAATTACAA CAAAAATATT ACAGCAGATT GAGGAACCAT TGGCATTGGC AAGTGGGGCT 
CTGCCAGACT GGTGTGAACA ATTAACCAGC AAATGTCCTT TTCTAATACC ATTTGAAACT 
AGACAGCTTT ATTTCACATG TACAGCATTT GGTGCCTCAA GAGCAATAGT GTGGTTACAA 
AACCGACGTG AAGCCACTGT GGAACGAACG AGAACCACAA GCAGTGTTAG GCGAGATGAC 
CCTGGAGAGT TTAGAGTTGG TCGTCTCAAG CATGAGAGAG TAAAAGTTCC ACGTGGCGAA 
TCACTGATGG AATGGGCTGA GAATGTCATG CAAATACATG CAGATCGGAA ATCAGTTCTT 
GAGGTTGAGT TTTTAGGAGA AGAAGGAACT GGCTTGGGAC CCACATTAGA GTTTTATGCT 
CTGGTGGCAG CGGAATTCCA GAGAACTGAC TTGGGAGCTT GGCTTTGTGA TGATAATTTT 
CCAGATGATG AATCTCGTCA CGTTGATCTT GGAGGTGGAT TGAAACCTCC TGGATATTAC 
GTGCAGAGGT CATGTGGACT GTTCACGGCA CCATTTCCAC AGGATAGTGA TGAGCTTGAA 
AGGATCACAA AACTGTTTCA TTTCCTTGGA ATTTTCTTGG CCAAATGCAT TCAAGACAAT 
AGACTTGTGG ACTTACCTAT TTCTAAACCT TTTTTTAAAC TTATGTGTAT GGGTGACATT 
AAAAGCAATA TGAGTAAACT GATTTATGAG TCACGAGGTG ATAGAGACTT ACACTGTACT 
GAAAGTCAGT CTGAAGCTTC TACAGAAGAA GGTCATGATT CACTCTCAGT AGGAAGCTTT 
GAAGAGGATT CAAAATCAGA ATTTATTCTT GATCCCCCTA AACCAAAACC CCCGGCTTGG 
TTTAATGGAA TTTTGACTTG GGAAGACTTT GAATTAGTAA ACCCACACAG AGCCAGATTT 
TTAAAAGAAA TTAAAGACCT TGCTATCAAG AGGCGCCAAA TTTTAAGCAA CAAAGGTCTT 
TCTGAAGATG AGAAGAACAC AAAATTACAG GAACTAGTGC TGAAGAATCC ATCAGGTTCT 
GGGCCTCCAC TTAGCATAGA GGATTTAGGT TTAAATTTCC AGTTTTGCCC TTCCTCAAGA 
ATATATGGTT TCACAGCTGT GGATCTCAAG CCAAGTGGTG AAGATGAGAT GATAACAATG 
GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC AGGTATTCAG 
AAACAAATGG AAGCCTTTAG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAGTTAAGT 
TCCTTCAGCC ATGAAGAAGT CCAAATGATT CTTTGTGGAA ACCAGTCACC ATCCTGGGCA 
GCAGAAGATA TTATCAATTA CACTGAACCT AAGCTGGGTT ATACACGTGA CAGCCCTGGT 
TTCCTGAGGT TTGTGAGGGT TTTATGTGGC ATGTCTTCTG ATGAAAGGAA AGCATTCTTG 
CAGTTTACCA CTGGTTGTTC AACTCTACCC CCAGGTGGAC TGGCTAACCT GCATCCCAGG 
CTCACGGTTG TACGCAAGGT TGATGCTACT GATGCAAGCT ATCCATCAGT CAATACATGT 
GTGCATTACC TTAAGTTGCC TGAATATTCT TCCGAGGAGA TCATGAGAGA GCGCCTGCTA 
GCTGCTACAA TGGAGAAAGG CTTTCATCTC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECTD1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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