ORF » Species Summary » Chelonia mydas » NEK1 cDNA ORF clone

NEK1 cDNA ORF clone, Chelonia mydas(Green sea turtle)

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh375622 XM_027829309.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375622 XM_027829311.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375622 XM_027829310.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375623 XM_027829312.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375624 XM_027829313.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375625 XM_027829314.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375626 XM_027829315.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375627 XM_027829316.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375628 XM_027829317.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375629 XM_027829318.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375630 XM_027829319.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OCh375631 XM_027829320.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCh375632 XM_027829321.1
Latest version!		 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OCh75990 XM_007067095.1
Latest version!		 Chelonia mydas NIMA-related kinase 1 (NEK1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NEK1 ( XM_027829309.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685110.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCh375622
    Clone ID Related Accession (Same CDS sequence) XM_027829309.1 , XM_027829310.1 , XM_027829311.1
    Accession Version XM_027829309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685110.1
    CDS104..4024
    Translation

    Target ORF information:

    RefSeq Version XM_027829309.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829310.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685111.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCh375622
    Clone ID Related Accession (Same CDS sequence) XM_027829309.1 , XM_027829310.1 , XM_027829311.1
    Accession Version XM_027829310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685111.1
    CDS62..3982
    Translation

    Target ORF information:

    RefSeq Version XM_027829310.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829311.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685112.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCh375622
    Clone ID Related Accession (Same CDS sequence) XM_027829309.1 , XM_027829310.1 , XM_027829311.1
    Accession Version XM_027829311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685112.1
    CDS46..3966
    Translation

    Target ORF information:

    RefSeq Version XM_027829311.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGGTCA TGCTTACACT 
    TGTCTCAGTG ACTCTATGCA ACCTGAACCC TTTTTCCAGA AGGTGGCACA GCCACAAGCC 
    ATACCAGCAG CCTCACCAGT TCTGTCAGCT CAGTCTTCAC TGGAAGAGCC TTTTCCACCT 
    CGTTCACGTT CTGTATCACC AGCAAAAAGT AAAGGCAAAA GTTCATTATT GATTGGGCTC 
    TCTACTGGCC TGTTTGATGC AAACAATCCA AAGATGTTAA GAACATGTTC ACTCCCAGAT 
    CTTTCCAAGT TGTACAGAAC ACTAATTGAC GTTCCCATTG TAGCAGATGT ACAACATCAA 
    GACAATCTTG AAATAGAGGA TATGGAAGAT GAGCATGTCA AAGAAGGGCA TTCTGATTCT 
    GAAGACATTA TGTTTGGTGA GGCAGATACA GACTTGCGGG CTCTTCAGGC CTCGATGGAA 
    CAGTTACTTA GGGAGCAGCC TAGTGAGGAA TTTAGTGAAG AGGAGTTGTC TACCTTAAAG 
    GCAGATACTG CAGCACGCAT AACAAATGGT ACAGAGTTGG ATGATGAGGA TGACAACAAC 
    CCCAGCAGTG AAAGTGTACT TAATGAAGAA TGGCAATCAG ACAACAGTGA TGGGGATATT 
    GCCAGTGAAT GTGAAGCATA TGACAGTGTC TTCAGCCATT TGGAAGAATT GAGATACAAT 
    CTGGAGCGGG AAATAGGCTT TGATAAATTC ATTGAAGTTT ATGATAAAAT AAAGGCAATA 
    CATGAAGATG AAGATGAGAA TATTGATGTT TGTTCAACAA TAGTTCAGAA TATTCTGGGA 
    AATGAATATA AACATCTGTA TGCCAAGATT CTTCATTTAG TGATGGCAGA TGGAGCCTAT 
    CAGGAGGATA ATGATGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829312.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685113.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OCh375623
    Clone ID Related Accession (Same CDS sequence) XM_027829312.1
    Accession Version XM_027829312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGACAC TTGTCTCAGT 
    GACTCTATGC AACCTGAACC CTTTTTCCAG AAGGTGGCAC AGCCACAAGC CATACCAGCA 
    GCCTCACCAG TTCTGTCAGC TCAGTCTTCA CTGGAAGAGC CTTTTCCACC TCGTTCACGT 
    TCTGTATCAC CAGCAAAAAG TAAAGGCAAA AGTTCATTAT TGATTGGGCT CTCTACTGGC 
    CTGTTTGATG CAAACAATCC AAAGATGTTA AGAACATGTT CACTCCCAGA TCTTTCCAAG 
    TTGTACAGAA CACTAATTGA CGTTCCCATT GTAGCAGATG TACAACATCA AGACAATCTT 
    GAAATAGAGG ATATGGAAGA TGAGCATGTC AAAGAAGGGC ATTCTGATTC TGAAGACATT 
    ATGTTTGGTG AGGCAGATAC AGACTTGCGG GCTCTTCAGG CCTCGATGGA ACAGTTACTT 
    AGGGAGCAGC CTAGTGAGGA ATTTAGTGAA GAGGAGTTGT CTACCTTAAA GGCAGATACT 
    GCAGCACGCA TAACAAATGG TACAGAGTTG GATGATGAGG ATGACAACAA CCCCAGCAGT 
    GAAAGTGTAC TTAATGAAGA ATGGCAATCA GACAACAGTG ATGGGGATAT TGCCAGTGAA 
    TGTGAAGCAT ATGACAGTGT CTTCAGCCAT TTGGAAGAAT TGAGATACAA TCTGGAGCGG 
    GAAATAGGCT TTGATAAATT CATTGAAGTT TATGATAAAA TAAAGGCAAT ACATGAAGAT 
    GAAGATGAGA ATATTGATGT TTGTTCAACA ATAGTTCAGA ATATTCTGGG AAATGAATAT 
    AAACATCTGT ATGCCAAGAT TCTTCATTTA GTGATGGCAG ATGGAGCCTA TCAGGAGGAT 
    AATGATGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685113.1
    CDS66..3977
    Translation

    Target ORF information:

    RefSeq Version XM_027829312.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGGTACACTG 
    CAAAACCTGG CAGCTATCTA TGGAGGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
    AACAAGGAGG AAGAGGAATA CCTGGCAAGA TTAAGACAGA TCCGACTTCA AAACTTCAAT 
    GAACGCCAGC AGATTAAAGC TAAACTTCGT GGAGAAAAGA ATGAAGCTGA CGGTTCTGAT 
    GGACAAGAAG GAAGTGAAGA AGCAGAATTG AGACGCAAAA AAATTGAAGC ACTAAAGGCC 
    CAAGCAAACG CGCGAGCTGC AGTTCTGAAA GAGCAACTAG AGCGAAAGAG GAAGGAGGCA 
    TATGAAAGAG AGAAGAAAGC TTGGGAAGAA CATTTGACAG CAAAAGGGGT AAAGAATCCT 
    ATGGTGCCTT TTAATGTGTC AGCAGCTGGG GTTAATCCTG TCCCTGGGCA ACAAGAACCT 
    GGAGGACCTC CTGCCAAACT ACAACTTCCA TTGAAGCCCA GTACACCACT TATATCTATG 
    ACTTCAGCTT TGAAGGAAGT GGGTGTGGAT GAAAATACAA CTGCTATCCA GGAAACCACA 
    GAGGAAACGA AGAAGGCAGA TCTTGCTGTG CAAAATAAAA GAGAAATACT AAGAAGATTG 
    AATCAAAATC TTAAAACACA GGACGATGTA AAAGGAATAA AGGAGCCTAA AGACACCTGT 
    GAGGCAGCTA TGGCTGAAGA GAGTAAAGAA GAAGAATATC CACTTCTAGT AGATCGCAAG 
    AAATGGGAGG TGGCAGCAGC TGAGTTGGTG GTTCCTCTTG CACAGCTAAC TATGGAGGAA 
    TCATTGTCGG GGACAGAGAG ACAGACCGTG GGGGAGGTAA TTAAACTAGA TGTTGGTGAG 
    CCCCAGAGAA AAGTTTGGGG CAAAAGCCCT ACTGATTCAG TTTTGAAGAT ACTGGGGGAA 
    GCAGAGCTAC AGCTTCAAAC TGAATTATTG GAGGAAATAA TTATCACAAA TGATGTTCCT 
    GAAGAAGGTT CTCAGCCCTT AACCGTGAAA GAGAACAGTG AAGTAGAAAT TGCTTGTATT 
    GGAGAGAAAT CTTCATCTCC AGTTGTTGGT GTCATAGAAT CTGAAAGGAC AATACCAGAG 
    GAGTCACAAA CATTTGATGC CACCCAAGTG CTGTCCAGGC CTGTTGTGCT ACATGAGCTG 
    TATGATTTGG AAGCAGAAAT GTTGGAAGAA ACTGAAAAAA ATAAAAAGTG CAAAAGTGAC 
    CAACTTCTCA TCACTGTTCA TGATGTGTGG GTTAAAGAAA GGGAAACAAT AACGGAGCAT 
    GAGCCACTGG AAGATACTGC CTGTCAGAAA GATGGTGGTG GTGATGAAGC AGCTTTGGAG 
    AAGGAAATGC CGCAAAATGT GCAACCGCGT AATGAAGTTT CTGCTGACAC TTGTCTCAGT 
    GACTCTATGC AACCTGAACC CTTTTTCCAG AAGGTGGCAC AGCCACAAGC CATACCAGCA 
    GCCTCACCAG TTCTGTCAGC TCAGTCTTCA CTGGAAGAGC CTTTTCCACC TCGTTCACGT 
    TCTGTATCAC CAGCAAAAAG TAAAGGCAAA AGTTCATTAT TGATTGGGCT CTCTACTGGC 
    CTGTTTGATG CAAACAATCC AAAGATGTTA AGAACATGTT CACTCCCAGA TCTTTCCAAG 
    TTGTACAGAA CACTAATTGA CGTTCCCATT GTAGCAGATG TACAACATCA AGACAATCTT 
    GAAATAGAGG ATATGGAAGA TGAGCATGTC AAAGAAGGGC ATTCTGATTC TGAAGACATT 
    ATGTTTGGTG AGGCAGATAC AGACTTGCGG GCTCTTCAGG CCTCGATGGA ACAGTTACTT 
    AGGGAGCAGC CTAGTGAGGA ATTTAGTGAA GAGGAGTTGT CTACCTTAAA GGCAGATACT 
    GCAGCACGCA TAACAAATGG TACAGAGTTG GATGATGAGG ATGACAACAA CCCCAGCAGT 
    GAAAGTGTAC TTAATGAAGA ATGGCAATCA GACAACAGTG ATGGGGATAT TGCCAGTGAA 
    TGTGAAGCAT ATGACAGTGT CTTCAGCCAT TTGGAAGAAT TGAGATACAA TCTGGAGCGG 
    GAAATAGGCT TTGATAAATT CATTGAAGTT TATGATAAAA TAAAGGCAAT ACATGAAGAT 
    GAAGATGAGA ATATTGATGT TTGTTCAACA ATAGTTCAGA ATATTCTGGG AAATGAATAT 
    AAACATCTGT ATGCCAAGAT TCTTCATTTA GTGATGGCAG ATGGAGCCTA TCAGGAGGAT 
    AATGATGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829313.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685114.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OCh375624
    Clone ID Related Accession (Same CDS sequence) XM_027829313.1
    Accession Version XM_027829313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGAATACCTG 
    GCAAGATTAA GACAGATCCG ACTTCAAAAC TTCAATGAAC GCCAGCAGAT TAAAGCTAAA 
    CTTCGTGGAG AAAAGAATGA AGCTGACGGT TCTGATGGAC AAGAAGGAAG TGAAGAAGCA 
    GAATTGAGAC GCAAAAAAAT TGAAGCACTA AAGGCCCAAG CAAACGCGCG AGCTGCAGTT 
    CTGAAAGAGC AACTAGAGCG AAAGAGGAAG GAGGCATATG AAAGAGAGAA GAAAGCTTGG 
    GAAGAACATT TGACAGCAAA AGGGGTAAAG AATCCTATGG TGCCTTTTAA TGTGTCAGCA 
    GCTGGGGTTA ATCCTGTCCC TGGGCAACAA GAACCTGGAG GACCTCCTGC CAAACTACAA 
    CTTCCATTGA AGCCCAGTAC ACCACTTATA TCTATGACTT CAGCTTTGAA GGAAGTGGGT 
    GTGGATGAAA ATACAACTGC TATCCAGGAA ACCACAGAGG AAACGAAGAA GGCAGATCTT 
    GCTGTGCAAA ATAAAAGAGA AATACTAAGA AGATTGAATC AAAATCTTAA AACACAGGAC 
    GATGTAAAAG GAATAAAGGA GCCTAAAGAC ACCTGTGAGG CAGCTATGGC TGAAGAGAGT 
    AAAGAAGAAG AATATCCACT TCTAGTAGAT CGCAAGAAAT GGGAGGTGGC AGCAGCTGAG 
    TTGGTGGTTC CTCTTGCACA GCTAACTATG GAGGAATCAT TGTCGGGGAC AGAGAGACAG 
    ACCGTGGGGG AGGTAATTAA ACTAGATGTT GGTGAGCCCC AGAGAAAAGT TTGGGGCAAA 
    AGCCCTACTG ATTCAGTTTT GAAGATACTG GGGGAAGCAG AGCTACAGCT TCAAACTGAA 
    TTATTGGAGG AAATAATTAT CACAAATGAT GTTCCTGAAG AAGGTTCTCA GCCCTTAACC 
    GTGAAAGAGA ACAGTGAAGT AGAAATTGCT TGTATTGGAG AGAAATCTTC ATCTCCAGTT 
    GTTGGTGTCA TAGAATCTGA AAGGACAATA CCAGAGGAGT CACAAACATT TGATGCCACC 
    CAAGTGCTGT CCAGGCCTGT TGTGCTACAT GAGCTGTATG ATTTGGAAGC AGAAATGTTG 
    GAAGAAACTG AAAAAAATAA AAAGTGCAAA AGTGACCAAC TTCTCATCAC TGTTCATGAT 
    GTGTGGGTTA AAGAAAGGGA AACAATAACG GAGCATGAGC CACTGGAAGA TACTGCCTGT 
    CAGAAAGATG GTGGTGGTGA TGAAGCAGCT TTGGAGAAGG AAATGCCGCA AAATGTGCAA 
    CCGCGTAATG AAGTTTCTGC TGGTCATGCT TACACTTGTC TCAGTGACTC TATGCAACCT 
    GAACCCTTTT TCCAGAAGGT GGCACAGCCA CAAGCCATAC CAGCAGCCTC ACCAGTTCTG 
    TCAGCTCAGT CTTCACTGGA AGAGCCTTTT CCACCTCGTT CACGTTCTGT ATCACCAGCA 
    AAAAGTAAAG GCAAAAGTTC ATTATTGATT GGGCTCTCTA CTGGCCTGTT TGATGCAAAC 
    AATCCAAAGA TGTTAAGAAC ATGTTCACTC CCAGATCTTT CCAAGTTGTA CAGAACACTA 
    ATTGACGTTC CCATTGTAGC AGATGTACAA CATCAAGACA ATCTTGAAAT AGAGGATATG 
    GAAGATGAGC ATGTCAAAGA AGGGCATTCT GATTCTGAAG ACATTATGTT TGGTGAGGCA 
    GATACAGACT TGCGGGCTCT TCAGGCCTCG ATGGAACAGT TACTTAGGGA GCAGCCTAGT 
    GAGGAATTTA GTGAAGAGGA GTTGTCTACC TTAAAGGCAG ATACTGCAGC ACGCATAACA 
    AATGGTACAG AGTTGGATGA TGAGGATGAC AACAACCCCA GCAGTGAAAG TGTACTTAAT 
    GAAGAATGGC AATCAGACAA CAGTGATGGG GATATTGCCA GTGAATGTGA AGCATATGAC 
    AGTGTCTTCA GCCATTTGGA AGAATTGAGA TACAATCTGG AGCGGGAAAT AGGCTTTGAT 
    AAATTCATTG AAGTTTATGA TAAAATAAAG GCAATACATG AAGATGAAGA TGAGAATATT 
    GATGTTTGTT CAACAATAGT TCAGAATATT CTGGGAAATG AATATAAACA TCTGTATGCC 
    AAGATTCTTC ATTTAGTGAT GGCAGATGGA GCCTATCAGG AGGATAATGA TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685114.1
    CDS104..3940
    Translation

    Target ORF information:

    RefSeq Version XM_027829313.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGAATACCTG 
    GCAAGATTAA GACAGATCCG ACTTCAAAAC TTCAATGAAC GCCAGCAGAT TAAAGCTAAA 
    CTTCGTGGAG AAAAGAATGA AGCTGACGGT TCTGATGGAC AAGAAGGAAG TGAAGAAGCA 
    GAATTGAGAC GCAAAAAAAT TGAAGCACTA AAGGCCCAAG CAAACGCGCG AGCTGCAGTT 
    CTGAAAGAGC AACTAGAGCG AAAGAGGAAG GAGGCATATG AAAGAGAGAA GAAAGCTTGG 
    GAAGAACATT TGACAGCAAA AGGGGTAAAG AATCCTATGG TGCCTTTTAA TGTGTCAGCA 
    GCTGGGGTTA ATCCTGTCCC TGGGCAACAA GAACCTGGAG GACCTCCTGC CAAACTACAA 
    CTTCCATTGA AGCCCAGTAC ACCACTTATA TCTATGACTT CAGCTTTGAA GGAAGTGGGT 
    GTGGATGAAA ATACAACTGC TATCCAGGAA ACCACAGAGG AAACGAAGAA GGCAGATCTT 
    GCTGTGCAAA ATAAAAGAGA AATACTAAGA AGATTGAATC AAAATCTTAA AACACAGGAC 
    GATGTAAAAG GAATAAAGGA GCCTAAAGAC ACCTGTGAGG CAGCTATGGC TGAAGAGAGT 
    AAAGAAGAAG AATATCCACT TCTAGTAGAT CGCAAGAAAT GGGAGGTGGC AGCAGCTGAG 
    TTGGTGGTTC CTCTTGCACA GCTAACTATG GAGGAATCAT TGTCGGGGAC AGAGAGACAG 
    ACCGTGGGGG AGGTAATTAA ACTAGATGTT GGTGAGCCCC AGAGAAAAGT TTGGGGCAAA 
    AGCCCTACTG ATTCAGTTTT GAAGATACTG GGGGAAGCAG AGCTACAGCT TCAAACTGAA 
    TTATTGGAGG AAATAATTAT CACAAATGAT GTTCCTGAAG AAGGTTCTCA GCCCTTAACC 
    GTGAAAGAGA ACAGTGAAGT AGAAATTGCT TGTATTGGAG AGAAATCTTC ATCTCCAGTT 
    GTTGGTGTCA TAGAATCTGA AAGGACAATA CCAGAGGAGT CACAAACATT TGATGCCACC 
    CAAGTGCTGT CCAGGCCTGT TGTGCTACAT GAGCTGTATG ATTTGGAAGC AGAAATGTTG 
    GAAGAAACTG AAAAAAATAA AAAGTGCAAA AGTGACCAAC TTCTCATCAC TGTTCATGAT 
    GTGTGGGTTA AAGAAAGGGA AACAATAACG GAGCATGAGC CACTGGAAGA TACTGCCTGT 
    CAGAAAGATG GTGGTGGTGA TGAAGCAGCT TTGGAGAAGG AAATGCCGCA AAATGTGCAA 
    CCGCGTAATG AAGTTTCTGC TGGTCATGCT TACACTTGTC TCAGTGACTC TATGCAACCT 
    GAACCCTTTT TCCAGAAGGT GGCACAGCCA CAAGCCATAC CAGCAGCCTC ACCAGTTCTG 
    TCAGCTCAGT CTTCACTGGA AGAGCCTTTT CCACCTCGTT CACGTTCTGT ATCACCAGCA 
    AAAAGTAAAG GCAAAAGTTC ATTATTGATT GGGCTCTCTA CTGGCCTGTT TGATGCAAAC 
    AATCCAAAGA TGTTAAGAAC ATGTTCACTC CCAGATCTTT CCAAGTTGTA CAGAACACTA 
    ATTGACGTTC CCATTGTAGC AGATGTACAA CATCAAGACA ATCTTGAAAT AGAGGATATG 
    GAAGATGAGC ATGTCAAAGA AGGGCATTCT GATTCTGAAG ACATTATGTT TGGTGAGGCA 
    GATACAGACT TGCGGGCTCT TCAGGCCTCG ATGGAACAGT TACTTAGGGA GCAGCCTAGT 
    GAGGAATTTA GTGAAGAGGA GTTGTCTACC TTAAAGGCAG ATACTGCAGC ACGCATAACA 
    AATGGTACAG AGTTGGATGA TGAGGATGAC AACAACCCCA GCAGTGAAAG TGTACTTAAT 
    GAAGAATGGC AATCAGACAA CAGTGATGGG GATATTGCCA GTGAATGTGA AGCATATGAC 
    AGTGTCTTCA GCCATTTGGA AGAATTGAGA TACAATCTGG AGCGGGAAAT AGGCTTTGAT 
    AAATTCATTG AAGTTTATGA TAAAATAAAG GCAATACATG AAGATGAAGA TGAGAATATT 
    GATGTTTGTT CAACAATAGT TCAGAATATT CTGGGAAATG AATATAAACA TCTGTATGCC 
    AAGATTCTTC ATTTAGTGAT GGCAGATGGA GCCTATCAGG AGGATAATGA TGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829314.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685115.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OCh375625
    Clone ID Related Accession (Same CDS sequence) XM_027829314.1
    Accession Version XM_027829314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGAATACCTG 
    GCAAGATTAA GACAGATCCG ACTTCAAAAC TTCAATGAAC GCCAGCAGAT TAAAGCTAAA 
    CTTCGTGGAG AAAAGAATGA AGCTGACGGT TCTGATGGAC AAGAAGGAAG TGAAGAAGCA 
    GAATTGAGAC GCAAAAAAAT TGAAGCACTA AAGGCCCAAG CAAACGCGCG AGCTGCAGTT 
    CTGAAAGAGC AACTAGAGCG AAAGAGGAAG GAGGCATATG AAAGAGAGAA GAAAGCTTGG 
    GAAGAACATT TGACAGCAAA AGGGGTAAAG AATCCTATGG TGCCTTTTAA TGTGTCAGCA 
    GCTGGGGTTA ATCCTGTCCC TGGGCAACAA GAACCTGGAG GACCTCCTGC CAAACTACAA 
    CTTCCATTGA AGCCCAGTAC ACCACTTATA TCTATGACTT CAGCTTTGAA GGAAGTGGGT 
    GTGGATGAAA ATACAACTGC TATCCAGGAA ACCACAGAGG AAACGAAGAA GGCAGATCTT 
    GCTGTGCAAA ATAAAAGAGA AATACTAAGA AGATTGAATC AAAATCTTAA AACACAGGAC 
    GATGTAAAAG GAATAAAGGA GCCTAAAGAC ACCTGTGAGG CAGCTATGGC TGAAGAGAGT 
    AAAGAAGAAG AATATCCACT TCTAGTAGAT CGCAAGAAAT GGGAGGTGGC AGCAGCTGAG 
    TTGGTGGTTC CTCTTGCACA GCTAACTATG GAGGAATCAT TGTCGGGGAC AGAGAGACAG 
    ACCGTGGGGG AGGTAATTAA ACTAGATGTT GGTGAGCCCC AGAGAAAAGT TTGGGGCAAA 
    AGCCCTACTG ATTCAGTTTT GAAGATACTG GGGGAAGCAG AGCTACAGCT TCAAACTGAA 
    TTATTGGAGG AAATAATTAT CACAAATGAT GTTCCTGAAG AAGGTTCTCA GCCCTTAACC 
    GTGAAAGAGA ACAGTGAAGT AGAAATTGCT TGTATTGGAG AGAAATCTTC ATCTCCAGTT 
    GTTGGTGTCA TAGAATCTGA AAGGACAATA CCAGAGGAGT CACAAACATT TGATGCCACC 
    CAAGTGCTGT CCAGGCCTGT TGTGCTACAT GAGCTGTATG ATTTGGAAGC AGAAATGTTG 
    GAAGAAACTG AAAAAAATAA AAAGTGCAAA AGTGACCAAC TTCTCATCAC TGTTCATGAT 
    GTGTGGGTTA AAGAAAGGGA AACAATAACG GAGCATGAGC CACTGGAAGA TACTGCCTGT 
    CAGAAAGATG GTGGTGGTGA TGAAGCAGCT TTGGAGAAGG AAATGCCGCA AAATGTGCAA 
    CCGCGTAATG AAGTTTCTGC TGACACTTGT CTCAGTGACT CTATGCAACC TGAACCCTTT 
    TTCCAGAAGG TGGCACAGCC ACAAGCCATA CCAGCAGCCT CACCAGTTCT GTCAGCTCAG 
    TCTTCACTGG AAGAGCCTTT TCCACCTCGT TCACGTTCTG TATCACCAGC AAAAAGTAAA 
    GGCAAAAGTT CATTATTGAT TGGGCTCTCT ACTGGCCTGT TTGATGCAAA CAATCCAAAG 
    ATGTTAAGAA CATGTTCACT CCCAGATCTT TCCAAGTTGT ACAGAACACT AATTGACGTT 
    CCCATTGTAG CAGATGTACA ACATCAAGAC AATCTTGAAA TAGAGGATAT GGAAGATGAG 
    CATGTCAAAG AAGGGCATTC TGATTCTGAA GACATTATGT TTGGTGAGGC AGATACAGAC 
    TTGCGGGCTC TTCAGGCCTC GATGGAACAG TTACTTAGGG AGCAGCCTAG TGAGGAATTT 
    AGTGAAGAGG AGTTGTCTAC CTTAAAGGCA GATACTGCAG CACGCATAAC AAATGGTACA 
    GAGTTGGATG ATGAGGATGA CAACAACCCC AGCAGTGAAA GTGTACTTAA TGAAGAATGG 
    CAATCAGACA ACAGTGATGG GGATATTGCC AGTGAATGTG AAGCATATGA CAGTGTCTTC 
    AGCCATTTGG AAGAATTGAG ATACAATCTG GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
    GAAGTTTATG ATAAAATAAA GGCAATACAT GAAGATGAAG ATGAGAATAT TGATGTTTGT 
    TCAACAATAG TTCAGAATAT TCTGGGAAAT GAATATAAAC ATCTGTATGC CAAGATTCTT 
    CATTTAGTGA TGGCAGATGG AGCCTATCAG GAGGATAATG ATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685115.1
    CDS66..3893
    Translation

    Target ORF information:

    RefSeq Version XM_027829314.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGAGGA 
    GTTCCACCTG GAGTACGTCC AGGATTTCCT AATGGGCCTG ATGGTCATCA CCATTTACCA 
    GATGCTGATG CAATTAGGAA AAAAATCAAA AGATTGAAGG AGGTGTCTAA ACAAGCCAAT 
    GCAAACAGGC AAAGAGGATG GGTAGCAGCA GAAAGAGCAA AACAAGTTGA AGAATTCTGG 
    CAACGAAAGA GGGAAGCTAT GCAAAACAAA GCTCGTGCTG AAGGACATAT GGAATACCTG 
    GCAAGATTAA GACAGATCCG ACTTCAAAAC TTCAATGAAC GCCAGCAGAT TAAAGCTAAA 
    CTTCGTGGAG AAAAGAATGA AGCTGACGGT TCTGATGGAC AAGAAGGAAG TGAAGAAGCA 
    GAATTGAGAC GCAAAAAAAT TGAAGCACTA AAGGCCCAAG CAAACGCGCG AGCTGCAGTT 
    CTGAAAGAGC AACTAGAGCG AAAGAGGAAG GAGGCATATG AAAGAGAGAA GAAAGCTTGG 
    GAAGAACATT TGACAGCAAA AGGGGTAAAG AATCCTATGG TGCCTTTTAA TGTGTCAGCA 
    GCTGGGGTTA ATCCTGTCCC TGGGCAACAA GAACCTGGAG GACCTCCTGC CAAACTACAA 
    CTTCCATTGA AGCCCAGTAC ACCACTTATA TCTATGACTT CAGCTTTGAA GGAAGTGGGT 
    GTGGATGAAA ATACAACTGC TATCCAGGAA ACCACAGAGG AAACGAAGAA GGCAGATCTT 
    GCTGTGCAAA ATAAAAGAGA AATACTAAGA AGATTGAATC AAAATCTTAA AACACAGGAC 
    GATGTAAAAG GAATAAAGGA GCCTAAAGAC ACCTGTGAGG CAGCTATGGC TGAAGAGAGT 
    AAAGAAGAAG AATATCCACT TCTAGTAGAT CGCAAGAAAT GGGAGGTGGC AGCAGCTGAG 
    TTGGTGGTTC CTCTTGCACA GCTAACTATG GAGGAATCAT TGTCGGGGAC AGAGAGACAG 
    ACCGTGGGGG AGGTAATTAA ACTAGATGTT GGTGAGCCCC AGAGAAAAGT TTGGGGCAAA 
    AGCCCTACTG ATTCAGTTTT GAAGATACTG GGGGAAGCAG AGCTACAGCT TCAAACTGAA 
    TTATTGGAGG AAATAATTAT CACAAATGAT GTTCCTGAAG AAGGTTCTCA GCCCTTAACC 
    GTGAAAGAGA ACAGTGAAGT AGAAATTGCT TGTATTGGAG AGAAATCTTC ATCTCCAGTT 
    GTTGGTGTCA TAGAATCTGA AAGGACAATA CCAGAGGAGT CACAAACATT TGATGCCACC 
    CAAGTGCTGT CCAGGCCTGT TGTGCTACAT GAGCTGTATG ATTTGGAAGC AGAAATGTTG 
    GAAGAAACTG AAAAAAATAA AAAGTGCAAA AGTGACCAAC TTCTCATCAC TGTTCATGAT 
    GTGTGGGTTA AAGAAAGGGA AACAATAACG GAGCATGAGC CACTGGAAGA TACTGCCTGT 
    CAGAAAGATG GTGGTGGTGA TGAAGCAGCT TTGGAGAAGG AAATGCCGCA AAATGTGCAA 
    CCGCGTAATG AAGTTTCTGC TGACACTTGT CTCAGTGACT CTATGCAACC TGAACCCTTT 
    TTCCAGAAGG TGGCACAGCC ACAAGCCATA CCAGCAGCCT CACCAGTTCT GTCAGCTCAG 
    TCTTCACTGG AAGAGCCTTT TCCACCTCGT TCACGTTCTG TATCACCAGC AAAAAGTAAA 
    GGCAAAAGTT CATTATTGAT TGGGCTCTCT ACTGGCCTGT TTGATGCAAA CAATCCAAAG 
    ATGTTAAGAA CATGTTCACT CCCAGATCTT TCCAAGTTGT ACAGAACACT AATTGACGTT 
    CCCATTGTAG CAGATGTACA ACATCAAGAC AATCTTGAAA TAGAGGATAT GGAAGATGAG 
    CATGTCAAAG AAGGGCATTC TGATTCTGAA GACATTATGT TTGGTGAGGC AGATACAGAC 
    TTGCGGGCTC TTCAGGCCTC GATGGAACAG TTACTTAGGG AGCAGCCTAG TGAGGAATTT 
    AGTGAAGAGG AGTTGTCTAC CTTAAAGGCA GATACTGCAG CACGCATAAC AAATGGTACA 
    GAGTTGGATG ATGAGGATGA CAACAACCCC AGCAGTGAAA GTGTACTTAA TGAAGAATGG 
    CAATCAGACA ACAGTGATGG GGATATTGCC AGTGAATGTG AAGCATATGA CAGTGTCTTC 
    AGCCATTTGG AAGAATTGAG ATACAATCTG GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
    GAAGTTTATG ATAAAATAAA GGCAATACAT GAAGATGAAG ATGAGAATAT TGATGTTTGT 
    TCAACAATAG TTCAGAATAT TCTGGGAAAT GAATATAAAC ATCTGTATGC CAAGATTCTT 
    CATTTAGTGA TGGCAGATGG AGCCTATCAG GAGGATAATG ATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829315.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685116.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OCh375626
    Clone ID Related Accession (Same CDS sequence) XM_027829315.1
    Accession Version XM_027829315.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG GTACACTGCA AAACCTGGCA 
    GCTATCTATG GAGGCAGGCC CATTTCTGCA AGAGGAAGGA AATCCAGAAA CAAGGAGGAA 
    GAGGAATACC TGGCAAGATT AAGACAGATC CGACTTCAAA ACTTCAATGA ACGCCAGCAG 
    ATTAAAGCTA AACTTCGTGG AGAAAAGAAT GAAGCTGACG GTTCTGATGG ACAAGAAGGA 
    AGTGAAGAAG CAGAATTGAG ACGCAAAAAA ATTGAAGCAC TAAAGGCCCA AGCAAACGCG 
    CGAGCTGCAG TTCTGAAAGA GCAACTAGAG CGAAAGAGGA AGGAGGCATA TGAAAGAGAG 
    AAGAAAGCTT GGGAAGAACA TTTGACAGCA AAAGGGGTAA AGAATCCTAT GGTGCCTTTT 
    AATGTGTCAG CAGCTGGGGT TAATCCTGTC CCTGGGCAAC AAGAACCTGG AGGACCTCCT 
    GCCAAACTAC AACTTCCATT GAAGCCCAGT ACACCACTTA TATCTATGAC TTCAGCTTTG 
    AAGGAAGTGG GTGTGGATGA AAATACAACT GCTATCCAGG AAACCACAGA GGAAACGAAG 
    AAGGCAGATC TTGCTGTGCA AAATAAAAGA GAAATACTAA GAAGATTGAA TCAAAATCTT 
    AAAACACAGG ACGATGTAAA AGGAATAAAG GAGCCTAAAG ACACCTGTGA GGCAGCTATG 
    GCTGAAGAGA GTAAAGAAGA AGAATATCCA CTTCTAGTAG ATCGCAAGAA ATGGGAGGTG 
    GCAGCAGCTG AGTTGGTGGT TCCTCTTGCA CAGCTAACTA TGGAGGAATC ATTGTCGGGG 
    ACAGAGAGAC AGACCGTGGG GGAGGTAATT AAACTAGATG TTGGTGAGCC CCAGAGAAAA 
    GTTTGGGGCA AAAGCCCTAC TGATTCAGTT TTGAAGATAC TGGGGGAAGC AGAGCTACAG 
    CTTCAAACTG AATTATTGGA GGAAATAATT ATCACAAATG ATGTTCCTGA AGAAGGTTCT 
    CAGCCCTTAA CCGTGAAAGA GAACAGTGAA GTAGAAATTG CTTGTATTGG AGAGAAATCT 
    TCATCTCCAG TTGTTGGTGT CATAGAATCT GAAAGGACAA TACCAGAGGA GTCACAAACA 
    TTTGATGCCA CCCAAGTGCT GTCCAGGCCT GTTGTGCTAC ATGAGCTGTA TGATTTGGAA 
    GCAGAAATGT TGGAAGAAAC TGAAAAAAAT AAAAAGTGCA AAAGTGACCA ACTTCTCATC 
    ACTGTTCATG ATGTGTGGGT TAAAGAAAGG GAAACAATAA CGGAGCATGA GCCACTGGAA 
    GATACTGCCT GTCAGAAAGA TGGTGGTGGT GATGAAGCAG CTTTGGAGAA GGAAATGCCG 
    CAAAATGTGC AACCGCGTAA TGAAGTTTCT GCTGGTCATG CTTACACTTG TCTCAGTGAC 
    TCTATGCAAC CTGAACCCTT TTTCCAGAAG GTGGCACAGC CACAAGCCAT ACCAGCAGCC 
    TCACCAGTTC TGTCAGCTCA GTCTTCACTG GAAGAGCCTT TTCCACCTCG TTCACGTTCT 
    GTATCACCAG CAAAAAGTAA AGGCAAAAGT TCATTATTGA TTGGGCTCTC TACTGGCCTG 
    TTTGATGCAA ACAATCCAAA GATGTTAAGA ACATGTTCAC TCCCAGATCT TTCCAAGTTG 
    TACAGAACAC TAATTGACGT TCCCATTGTA GCAGATGTAC AACATCAAGA CAATCTTGAA 
    ATAGAGGATA TGGAAGATGA GCATGTCAAA GAAGGGCATT CTGATTCTGA AGACATTATG 
    TTTGGTGAGG CAGATACAGA CTTGCGGGCT CTTCAGGCCT CGATGGAACA GTTACTTAGG 
    GAGCAGCCTA GTGAGGAATT TAGTGAAGAG GAGTTGTCTA CCTTAAAGGC AGATACTGCA 
    GCACGCATAA CAAATGGTAC AGAGTTGGAT GATGAGGATG ACAACAACCC CAGCAGTGAA 
    AGTGTACTTA ATGAAGAATG GCAATCAGAC AACAGTGATG GGGATATTGC CAGTGAATGT 
    GAAGCATATG ACAGTGTCTT CAGCCATTTG GAAGAATTGA GATACAATCT GGAGCGGGAA 
    ATAGGCTTTG ATAAATTCAT TGAAGTTTAT GATAAAATAA AGGCAATACA TGAAGATGAA 
    GATGAGAATA TTGATGTTTG TTCAACAATA GTTCAGAATA TTCTGGGAAA TGAATATAAA 
    CATCTGTATG CCAAGATTCT TCATTTAGTG ATGGCAGATG GAGCCTATCA GGAGGATAAT 
    GATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685116.1
    CDS104..3892
    Translation

    Target ORF information:

    RefSeq Version XM_027829315.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829315.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG GTACACTGCA AAACCTGGCA 
    GCTATCTATG GAGGCAGGCC CATTTCTGCA AGAGGAAGGA AATCCAGAAA CAAGGAGGAA 
    GAGGAATACC TGGCAAGATT AAGACAGATC CGACTTCAAA ACTTCAATGA ACGCCAGCAG 
    ATTAAAGCTA AACTTCGTGG AGAAAAGAAT GAAGCTGACG GTTCTGATGG ACAAGAAGGA 
    AGTGAAGAAG CAGAATTGAG ACGCAAAAAA ATTGAAGCAC TAAAGGCCCA AGCAAACGCG 
    CGAGCTGCAG TTCTGAAAGA GCAACTAGAG CGAAAGAGGA AGGAGGCATA TGAAAGAGAG 
    AAGAAAGCTT GGGAAGAACA TTTGACAGCA AAAGGGGTAA AGAATCCTAT GGTGCCTTTT 
    AATGTGTCAG CAGCTGGGGT TAATCCTGTC CCTGGGCAAC AAGAACCTGG AGGACCTCCT 
    GCCAAACTAC AACTTCCATT GAAGCCCAGT ACACCACTTA TATCTATGAC TTCAGCTTTG 
    AAGGAAGTGG GTGTGGATGA AAATACAACT GCTATCCAGG AAACCACAGA GGAAACGAAG 
    AAGGCAGATC TTGCTGTGCA AAATAAAAGA GAAATACTAA GAAGATTGAA TCAAAATCTT 
    AAAACACAGG ACGATGTAAA AGGAATAAAG GAGCCTAAAG ACACCTGTGA GGCAGCTATG 
    GCTGAAGAGA GTAAAGAAGA AGAATATCCA CTTCTAGTAG ATCGCAAGAA ATGGGAGGTG 
    GCAGCAGCTG AGTTGGTGGT TCCTCTTGCA CAGCTAACTA TGGAGGAATC ATTGTCGGGG 
    ACAGAGAGAC AGACCGTGGG GGAGGTAATT AAACTAGATG TTGGTGAGCC CCAGAGAAAA 
    GTTTGGGGCA AAAGCCCTAC TGATTCAGTT TTGAAGATAC TGGGGGAAGC AGAGCTACAG 
    CTTCAAACTG AATTATTGGA GGAAATAATT ATCACAAATG ATGTTCCTGA AGAAGGTTCT 
    CAGCCCTTAA CCGTGAAAGA GAACAGTGAA GTAGAAATTG CTTGTATTGG AGAGAAATCT 
    TCATCTCCAG TTGTTGGTGT CATAGAATCT GAAAGGACAA TACCAGAGGA GTCACAAACA 
    TTTGATGCCA CCCAAGTGCT GTCCAGGCCT GTTGTGCTAC ATGAGCTGTA TGATTTGGAA 
    GCAGAAATGT TGGAAGAAAC TGAAAAAAAT AAAAAGTGCA AAAGTGACCA ACTTCTCATC 
    ACTGTTCATG ATGTGTGGGT TAAAGAAAGG GAAACAATAA CGGAGCATGA GCCACTGGAA 
    GATACTGCCT GTCAGAAAGA TGGTGGTGGT GATGAAGCAG CTTTGGAGAA GGAAATGCCG 
    CAAAATGTGC AACCGCGTAA TGAAGTTTCT GCTGGTCATG CTTACACTTG TCTCAGTGAC 
    TCTATGCAAC CTGAACCCTT TTTCCAGAAG GTGGCACAGC CACAAGCCAT ACCAGCAGCC 
    TCACCAGTTC TGTCAGCTCA GTCTTCACTG GAAGAGCCTT TTCCACCTCG TTCACGTTCT 
    GTATCACCAG CAAAAAGTAA AGGCAAAAGT TCATTATTGA TTGGGCTCTC TACTGGCCTG 
    TTTGATGCAA ACAATCCAAA GATGTTAAGA ACATGTTCAC TCCCAGATCT TTCCAAGTTG 
    TACAGAACAC TAATTGACGT TCCCATTGTA GCAGATGTAC AACATCAAGA CAATCTTGAA 
    ATAGAGGATA TGGAAGATGA GCATGTCAAA GAAGGGCATT CTGATTCTGA AGACATTATG 
    TTTGGTGAGG CAGATACAGA CTTGCGGGCT CTTCAGGCCT CGATGGAACA GTTACTTAGG 
    GAGCAGCCTA GTGAGGAATT TAGTGAAGAG GAGTTGTCTA CCTTAAAGGC AGATACTGCA 
    GCACGCATAA CAAATGGTAC AGAGTTGGAT GATGAGGATG ACAACAACCC CAGCAGTGAA 
    AGTGTACTTA ATGAAGAATG GCAATCAGAC AACAGTGATG GGGATATTGC CAGTGAATGT 
    GAAGCATATG ACAGTGTCTT CAGCCATTTG GAAGAATTGA GATACAATCT GGAGCGGGAA 
    ATAGGCTTTG ATAAATTCAT TGAAGTTTAT GATAAAATAA AGGCAATACA TGAAGATGAA 
    GATGAGAATA TTGATGTTTG TTCAACAATA GTTCAGAATA TTCTGGGAAA TGAATATAAA 
    CATCTGTATG CCAAGATTCT TCATTTAGTG ATGGCAGATG GAGCCTATCA GGAGGATAAT 
    GATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829316.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685117.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OCh375627
    Clone ID Related Accession (Same CDS sequence) XM_027829316.1
    Accession Version XM_027829316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG GTACACTGCA AAACCTGGCA 
    GCTATCTATG GAGGCAGGCC CATTTCTGCA AGAGGAAGGA AATCCAGAAA CAAGGAGGAA 
    GAGGAATACC TGGCAAGATT AAGACAGATC CGACTTCAAA ACTTCAATGA ACGCCAGCAG 
    ATTAAAGCTA AACTTCGTGG AGAAAAGAAT GAAGCTGACG GTTCTGATGG ACAAGAAGGA 
    AGTGAAGAAG CAGAATTGAG ACGCAAAAAA ATTGAAGCAC TAAAGGCCCA AGCAAACGCG 
    CGAGCTGCAG TTCTGAAAGA GCAACTAGAG CGAAAGAGGA AGGAGGCATA TGAAAGAGAG 
    AAGAAAGCTT GGGAAGAACA TTTGACAGCA AAAGGGGTAA AGAATCCTAT GGTGCCTTTT 
    AATGTGTCAG CAGCTGGGGT TAATCCTGTC CCTGGGCAAC AAGAACCTGG AGGACCTCCT 
    GCCAAACTAC AACTTCCATT GAAGCCCAGT ACACCACTTA TATCTATGAC TTCAGCTTTG 
    AAGGAAGTGG GTGTGGATGA AAATACAACT GCTATCCAGG AAACCACAGA GGAAACGAAG 
    AAGGCAGATC TTGCTGTGCA AAATAAAAGA GAAATACTAA GAAGATTGAA TCAAAATCTT 
    AAAACACAGG ACGATGTAAA AGGAATAAAG GAGCCTAAAG ACACCTGTGA GGCAGCTATG 
    GCTGAAGAGA GTAAAGAAGA AGAATATCCA CTTCTAGTAG ATCGCAAGAA ATGGGAGGTG 
    GCAGCAGCTG AGTTGGTGGT TCCTCTTGCA CAGCTAACTA TGGAGGAATC ATTGTCGGGG 
    ACAGAGAGAC AGACCGTGGG GGAGGTAATT AAACTAGATG TTGGTGAGCC CCAGAGAAAA 
    GTTTGGGGCA AAAGCCCTAC TGATTCAGTT TTGAAGATAC TGGGGGAAGC AGAGCTACAG 
    CTTCAAACTG AATTATTGGA GGAAATAATT ATCACAAATG ATGTTCCTGA AGAAGGTTCT 
    CAGCCCTTAA CCGTGAAAGA GAACAGTGAA GTAGAAATTG CTTGTATTGG AGAGAAATCT 
    TCATCTCCAG TTGTTGGTGT CATAGAATCT GAAAGGACAA TACCAGAGGA GTCACAAACA 
    TTTGATGCCA CCCAAGTGCT GTCCAGGCCT GTTGTGCTAC ATGAGCTGTA TGATTTGGAA 
    GCAGAAATGT TGGAAGAAAC TGAAAAAAAT AAAAAGTGCA AAAGTGACCA ACTTCTCATC 
    ACTGTTCATG ATGTGTGGGT TAAAGAAAGG GAAACAATAA CGGAGCATGA GCCACTGGAA 
    GATACTGCCT GTCAGAAAGA TGGTGGTGGT GATGAAGCAG CTTTGGAGAA GGAAATGCCG 
    CAAAATGTGC AACCGCGTAA TGAAGTTTCT GCTGACACTT GTCTCAGTGA CTCTATGCAA 
    CCTGAACCCT TTTTCCAGAA GGTGGCACAG CCACAAGCCA TACCAGCAGC CTCACCAGTT 
    CTGTCAGCTC AGTCTTCACT GGAAGAGCCT TTTCCACCTC GTTCACGTTC TGTATCACCA 
    GCAAAAAGTA AAGGCAAAAG TTCATTATTG ATTGGGCTCT CTACTGGCCT GTTTGATGCA 
    AACAATCCAA AGATGTTAAG AACATGTTCA CTCCCAGATC TTTCCAAGTT GTACAGAACA 
    CTAATTGACG TTCCCATTGT AGCAGATGTA CAACATCAAG ACAATCTTGA AATAGAGGAT 
    ATGGAAGATG AGCATGTCAA AGAAGGGCAT TCTGATTCTG AAGACATTAT GTTTGGTGAG 
    GCAGATACAG ACTTGCGGGC TCTTCAGGCC TCGATGGAAC AGTTACTTAG GGAGCAGCCT 
    AGTGAGGAAT TTAGTGAAGA GGAGTTGTCT ACCTTAAAGG CAGATACTGC AGCACGCATA 
    ACAAATGGTA CAGAGTTGGA TGATGAGGAT GACAACAACC CCAGCAGTGA AAGTGTACTT 
    AATGAAGAAT GGCAATCAGA CAACAGTGAT GGGGATATTG CCAGTGAATG TGAAGCATAT 
    GACAGTGTCT TCAGCCATTT GGAAGAATTG AGATACAATC TGGAGCGGGA AATAGGCTTT 
    GATAAATTCA TTGAAGTTTA TGATAAAATA AAGGCAATAC ATGAAGATGA AGATGAGAAT 
    ATTGATGTTT GTTCAACAAT AGTTCAGAAT ATTCTGGGAA ATGAATATAA ACATCTGTAT 
    GCCAAGATTC TTCATTTAGT GATGGCAGAT GGAGCCTATC AGGAGGATAA TGATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685117.1
    CDS66..3845
    Translation

    Target ORF information:

    RefSeq Version XM_027829316.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG GTACACTGCA AAACCTGGCA 
    GCTATCTATG GAGGCAGGCC CATTTCTGCA AGAGGAAGGA AATCCAGAAA CAAGGAGGAA 
    GAGGAATACC TGGCAAGATT AAGACAGATC CGACTTCAAA ACTTCAATGA ACGCCAGCAG 
    ATTAAAGCTA AACTTCGTGG AGAAAAGAAT GAAGCTGACG GTTCTGATGG ACAAGAAGGA 
    AGTGAAGAAG CAGAATTGAG ACGCAAAAAA ATTGAAGCAC TAAAGGCCCA AGCAAACGCG 
    CGAGCTGCAG TTCTGAAAGA GCAACTAGAG CGAAAGAGGA AGGAGGCATA TGAAAGAGAG 
    AAGAAAGCTT GGGAAGAACA TTTGACAGCA AAAGGGGTAA AGAATCCTAT GGTGCCTTTT 
    AATGTGTCAG CAGCTGGGGT TAATCCTGTC CCTGGGCAAC AAGAACCTGG AGGACCTCCT 
    GCCAAACTAC AACTTCCATT GAAGCCCAGT ACACCACTTA TATCTATGAC TTCAGCTTTG 
    AAGGAAGTGG GTGTGGATGA AAATACAACT GCTATCCAGG AAACCACAGA GGAAACGAAG 
    AAGGCAGATC TTGCTGTGCA AAATAAAAGA GAAATACTAA GAAGATTGAA TCAAAATCTT 
    AAAACACAGG ACGATGTAAA AGGAATAAAG GAGCCTAAAG ACACCTGTGA GGCAGCTATG 
    GCTGAAGAGA GTAAAGAAGA AGAATATCCA CTTCTAGTAG ATCGCAAGAA ATGGGAGGTG 
    GCAGCAGCTG AGTTGGTGGT TCCTCTTGCA CAGCTAACTA TGGAGGAATC ATTGTCGGGG 
    ACAGAGAGAC AGACCGTGGG GGAGGTAATT AAACTAGATG TTGGTGAGCC CCAGAGAAAA 
    GTTTGGGGCA AAAGCCCTAC TGATTCAGTT TTGAAGATAC TGGGGGAAGC AGAGCTACAG 
    CTTCAAACTG AATTATTGGA GGAAATAATT ATCACAAATG ATGTTCCTGA AGAAGGTTCT 
    CAGCCCTTAA CCGTGAAAGA GAACAGTGAA GTAGAAATTG CTTGTATTGG AGAGAAATCT 
    TCATCTCCAG TTGTTGGTGT CATAGAATCT GAAAGGACAA TACCAGAGGA GTCACAAACA 
    TTTGATGCCA CCCAAGTGCT GTCCAGGCCT GTTGTGCTAC ATGAGCTGTA TGATTTGGAA 
    GCAGAAATGT TGGAAGAAAC TGAAAAAAAT AAAAAGTGCA AAAGTGACCA ACTTCTCATC 
    ACTGTTCATG ATGTGTGGGT TAAAGAAAGG GAAACAATAA CGGAGCATGA GCCACTGGAA 
    GATACTGCCT GTCAGAAAGA TGGTGGTGGT GATGAAGCAG CTTTGGAGAA GGAAATGCCG 
    CAAAATGTGC AACCGCGTAA TGAAGTTTCT GCTGACACTT GTCTCAGTGA CTCTATGCAA 
    CCTGAACCCT TTTTCCAGAA GGTGGCACAG CCACAAGCCA TACCAGCAGC CTCACCAGTT 
    CTGTCAGCTC AGTCTTCACT GGAAGAGCCT TTTCCACCTC GTTCACGTTC TGTATCACCA 
    GCAAAAAGTA AAGGCAAAAG TTCATTATTG ATTGGGCTCT CTACTGGCCT GTTTGATGCA 
    AACAATCCAA AGATGTTAAG AACATGTTCA CTCCCAGATC TTTCCAAGTT GTACAGAACA 
    CTAATTGACG TTCCCATTGT AGCAGATGTA CAACATCAAG ACAATCTTGA AATAGAGGAT 
    ATGGAAGATG AGCATGTCAA AGAAGGGCAT TCTGATTCTG AAGACATTAT GTTTGGTGAG 
    GCAGATACAG ACTTGCGGGC TCTTCAGGCC TCGATGGAAC AGTTACTTAG GGAGCAGCCT 
    AGTGAGGAAT TTAGTGAAGA GGAGTTGTCT ACCTTAAAGG CAGATACTGC AGCACGCATA 
    ACAAATGGTA CAGAGTTGGA TGATGAGGAT GACAACAACC CCAGCAGTGA AAGTGTACTT 
    AATGAAGAAT GGCAATCAGA CAACAGTGAT GGGGATATTG CCAGTGAATG TGAAGCATAT 
    GACAGTGTCT TCAGCCATTT GGAAGAATTG AGATACAATC TGGAGCGGGA AATAGGCTTT 
    GATAAATTCA TTGAAGTTTA TGATAAAATA AAGGCAATAC ATGAAGATGA AGATGAGAAT 
    ATTGATGTTT GTTCAACAAT AGTTCAGAAT ATTCTGGGAA ATGAATATAA ACATCTGTAT 
    GCCAAGATTC TTCATTTAGT GATGGCAGAT GGAGCCTATC AGGAGGATAA TGATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829317.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685118.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OCh375628
    Clone ID Related Accession (Same CDS sequence) XM_027829317.1
    Accession Version XM_027829317.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG AATACCTGGC AAGATTAAGA 
    CAGATCCGAC TTCAAAACTT CAATGAACGC CAGCAGATTA AAGCTAAACT TCGTGGAGAA 
    AAGAATGAAG CTGACGGTTC TGATGGACAA GAAGGAAGTG AAGAAGCAGA ATTGAGACGC 
    AAAAAAATTG AAGCACTAAA GGCCCAAGCA AACGCGCGAG CTGCAGTTCT GAAAGAGCAA 
    CTAGAGCGAA AGAGGAAGGA GGCATATGAA AGAGAGAAGA AAGCTTGGGA AGAACATTTG 
    ACAGCAAAAG GGGTAAAGAA TCCTATGGTG CCTTTTAATG TGTCAGCAGC TGGGGTTAAT 
    CCTGTCCCTG GGCAACAAGA ACCTGGAGGA CCTCCTGCCA AACTACAACT TCCATTGAAG 
    CCCAGTACAC CACTTATATC TATGACTTCA GCTTTGAAGG AAGTGGGTGT GGATGAAAAT 
    ACAACTGCTA TCCAGGAAAC CACAGAGGAA ACGAAGAAGG CAGATCTTGC TGTGCAAAAT 
    AAAAGAGAAA TACTAAGAAG ATTGAATCAA AATCTTAAAA CACAGGACGA TGTAAAAGGA 
    ATAAAGGAGC CTAAAGACAC CTGTGAGGCA GCTATGGCTG AAGAGAGTAA AGAAGAAGAA 
    TATCCACTTC TAGTAGATCG CAAGAAATGG GAGGTGGCAG CAGCTGAGTT GGTGGTTCCT 
    CTTGCACAGC TAACTATGGA GGAATCATTG TCGGGGACAG AGAGACAGAC CGTGGGGGAG 
    GTAATTAAAC TAGATGTTGG TGAGCCCCAG AGAAAAGTTT GGGGCAAAAG CCCTACTGAT 
    TCAGTTTTGA AGATACTGGG GGAAGCAGAG CTACAGCTTC AAACTGAATT ATTGGAGGAA 
    ATAATTATCA CAAATGATGT TCCTGAAGAA GGTTCTCAGC CCTTAACCGT GAAAGAGAAC 
    AGTGAAGTAG AAATTGCTTG TATTGGAGAG AAATCTTCAT CTCCAGTTGT TGGTGTCATA 
    GAATCTGAAA GGACAATACC AGAGGAGTCA CAAACATTTG ATGCCACCCA AGTGCTGTCC 
    AGGCCTGTTG TGCTACATGA GCTGTATGAT TTGGAAGCAG AAATGTTGGA AGAAACTGAA 
    AAAAATAAAA AGTGCAAAAG TGACCAACTT CTCATCACTG TTCATGATGT GTGGGTTAAA 
    GAAAGGGAAA CAATAACGGA GCATGAGCCA CTGGAAGATA CTGCCTGTCA GAAAGATGGT 
    GGTGGTGATG AAGCAGCTTT GGAGAAGGAA ATGCCGCAAA ATGTGCAACC GCGTAATGAA 
    GTTTCTGCTG GTCATGCTTA CACTTGTCTC AGTGACTCTA TGCAACCTGA ACCCTTTTTC 
    CAGAAGGTGG CACAGCCACA AGCCATACCA GCAGCCTCAC CAGTTCTGTC AGCTCAGTCT 
    TCACTGGAAG AGCCTTTTCC ACCTCGTTCA CGTTCTGTAT CACCAGCAAA AAGTAAAGGC 
    AAAAGTTCAT TATTGATTGG GCTCTCTACT GGCCTGTTTG ATGCAAACAA TCCAAAGATG 
    TTAAGAACAT GTTCACTCCC AGATCTTTCC AAGTTGTACA GAACACTAAT TGACGTTCCC 
    ATTGTAGCAG ATGTACAACA TCAAGACAAT CTTGAAATAG AGGATATGGA AGATGAGCAT 
    GTCAAAGAAG GGCATTCTGA TTCTGAAGAC ATTATGTTTG GTGAGGCAGA TACAGACTTG 
    CGGGCTCTTC AGGCCTCGAT GGAACAGTTA CTTAGGGAGC AGCCTAGTGA GGAATTTAGT 
    GAAGAGGAGT TGTCTACCTT AAAGGCAGAT ACTGCAGCAC GCATAACAAA TGGTACAGAG 
    TTGGATGATG AGGATGACAA CAACCCCAGC AGTGAAAGTG TACTTAATGA AGAATGGCAA 
    TCAGACAACA GTGATGGGGA TATTGCCAGT GAATGTGAAG CATATGACAG TGTCTTCAGC 
    CATTTGGAAG AATTGAGATA CAATCTGGAG CGGGAAATAG GCTTTGATAA ATTCATTGAA 
    GTTTATGATA AAATAAAGGC AATACATGAA GATGAAGATG AGAATATTGA TGTTTGTTCA 
    ACAATAGTTC AGAATATTCT GGGAAATGAA TATAAACATC TGTATGCCAA GATTCTTCAT 
    TTAGTGATGG CAGATGGAGC CTATCAGGAG GATAATGATG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685118.1
    CDS104..3808
    Translation

    Target ORF information:

    RefSeq Version XM_027829317.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829317.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGAAAT ATATTAAAGT GCAGAAGATT GGCGAAGGAT CCTTTGGAAA AGCAATTCTG 
    GTCAAAGCTA AAGAAAATAG CAGACAGTAT GTTGTTAAGG AGATAAACAT CTCTAAGATG 
    TCCAATAAAG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAACAT 
    CCAAATATTG TCCTGTATCG GGAATCATTT GAAGAAAATG GCTCTCTCTA TATAGTGATG 
    GATTACTGTG AAGGAGGGGA CCTGTTTAGA CGAATAAATG CTCAGAAAGG CATCTTGTTC 
    TCAGAGGATC AGATAATGGA TTGGTTTGTA CAGATTTGTT TAGCACTAAA ACATGTACAT 
    GACAGAAAAA TTTTGCATCG AGATATAAAG TCACAGAATA TATTTTTAAC CAAAGATGGA 
    ACAATACAAC TGGGAGACTT TGGAATAGCT AGAGTTCTTA ACAGTACTGT AGAGCTGGCT 
    CGCACTTGCA TAGGAACACC ATACTATTTG TCACCTGAAA TCTGTCGGAA CAAGCCTTAC 
    AACAATAAAA GTGACATATG GGCTCTTGGT TGTGTTCTCT ATGAGATGTG CACACTTAAA 
    CATGCATTTG AAGCTGGTAA CATGAAAAAT CTGGTACTGA AGATAATCTC TGGATCTTTT 
    CCTCCTGTTT CTCTGCATTA TTCCTACGAT CTACGTAATC TGCTTTCACA GTTATTTAAA 
    AGGAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAATCTT TATAGCCAAA 
    CGGGTGGAAA AATTTCTTAC ACCACAGCTT ATTGCAGACG AATTCAGTCA CAAAGTGTTC 
    CACAAGTTTG ACGCCCATGT TGCACCAGTA AAACGACTAG CTCAAGGACA AATCTGTGCT 
    TCAGCTGTGC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAATG 
    ATCAAGAAAT CTGGTGATGC ATCTAAAAAA TTTCATGAGA AGAAGCCATT AGCTAAATTT 
    AGACAGGCCC ATCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
    TTTGAGGAAG CTGCAAAAAA GAGAAGATTT GAATTACTTG AGAAAGAAAA ACGACAAAGA 
    GACCAGAAGG CTGAGCAGAT GAGAAGACTG GAAAAGGAAA GGATGGAACG GATAAACAGA 
    GCCAGGGAAC AAGGCTGGAG AAATGTGCTT AGTTCAGGTG GAAGTGGTGA AGTTAAGGCA 
    CCCTTTTTTG GCGGTGGAGG AGGTGTTGGG CCTTCCCCTG TGCCTGCCAG AGGACAGTAT 
    GAACACTACC ATGCTATCTT TGACCAGATG CAACAGCAAA AGGAAAATAT TAAAGTAGGT 
    GAGGAGAGAG AAACCAGATG GAGAGCAGAA ATACGAAGTC GGGAAGCTGT TGAAAGGCAA 
    AGAGGATGGG TAGCAGCAGA AAGAGCAAAA CAAGTTGAAG AATTCTGGCA ACGAAAGAGG 
    GAAGCTATGC AAAACAAAGC TCGTGCTGAA GGACATATGG AATACCTGGC AAGATTAAGA 
    CAGATCCGAC TTCAAAACTT CAATGAACGC CAGCAGATTA AAGCTAAACT TCGTGGAGAA 
    AAGAATGAAG CTGACGGTTC TGATGGACAA GAAGGAAGTG AAGAAGCAGA ATTGAGACGC 
    AAAAAAATTG AAGCACTAAA GGCCCAAGCA AACGCGCGAG CTGCAGTTCT GAAAGAGCAA 
    CTAGAGCGAA AGAGGAAGGA GGCATATGAA AGAGAGAAGA AAGCTTGGGA AGAACATTTG 
    ACAGCAAAAG GGGTAAAGAA TCCTATGGTG CCTTTTAATG TGTCAGCAGC TGGGGTTAAT 
    CCTGTCCCTG GGCAACAAGA ACCTGGAGGA CCTCCTGCCA AACTACAACT TCCATTGAAG 
    CCCAGTACAC CACTTATATC TATGACTTCA GCTTTGAAGG AAGTGGGTGT GGATGAAAAT 
    ACAACTGCTA TCCAGGAAAC CACAGAGGAA ACGAAGAAGG CAGATCTTGC TGTGCAAAAT 
    AAAAGAGAAA TACTAAGAAG ATTGAATCAA AATCTTAAAA CACAGGACGA TGTAAAAGGA 
    ATAAAGGAGC CTAAAGACAC CTGTGAGGCA GCTATGGCTG AAGAGAGTAA AGAAGAAGAA 
    TATCCACTTC TAGTAGATCG CAAGAAATGG GAGGTGGCAG CAGCTGAGTT GGTGGTTCCT 
    CTTGCACAGC TAACTATGGA GGAATCATTG TCGGGGACAG AGAGACAGAC CGTGGGGGAG 
    GTAATTAAAC TAGATGTTGG TGAGCCCCAG AGAAAAGTTT GGGGCAAAAG CCCTACTGAT 
    TCAGTTTTGA AGATACTGGG GGAAGCAGAG CTACAGCTTC AAACTGAATT ATTGGAGGAA 
    ATAATTATCA CAAATGATGT TCCTGAAGAA GGTTCTCAGC CCTTAACCGT GAAAGAGAAC 
    AGTGAAGTAG AAATTGCTTG TATTGGAGAG AAATCTTCAT CTCCAGTTGT TGGTGTCATA 
    GAATCTGAAA GGACAATACC AGAGGAGTCA CAAACATTTG ATGCCACCCA AGTGCTGTCC 
    AGGCCTGTTG TGCTACATGA GCTGTATGAT TTGGAAGCAG AAATGTTGGA AGAAACTGAA 
    AAAAATAAAA AGTGCAAAAG TGACCAACTT CTCATCACTG TTCATGATGT GTGGGTTAAA 
    GAAAGGGAAA CAATAACGGA GCATGAGCCA CTGGAAGATA CTGCCTGTCA GAAAGATGGT 
    GGTGGTGATG AAGCAGCTTT GGAGAAGGAA ATGCCGCAAA ATGTGCAACC GCGTAATGAA 
    GTTTCTGCTG GTCATGCTTA CACTTGTCTC AGTGACTCTA TGCAACCTGA ACCCTTTTTC 
    CAGAAGGTGG CACAGCCACA AGCCATACCA GCAGCCTCAC CAGTTCTGTC AGCTCAGTCT 
    TCACTGGAAG AGCCTTTTCC ACCTCGTTCA CGTTCTGTAT CACCAGCAAA AAGTAAAGGC 
    AAAAGTTCAT TATTGATTGG GCTCTCTACT GGCCTGTTTG ATGCAAACAA TCCAAAGATG 
    TTAAGAACAT GTTCACTCCC AGATCTTTCC AAGTTGTACA GAACACTAAT TGACGTTCCC 
    ATTGTAGCAG ATGTACAACA TCAAGACAAT CTTGAAATAG AGGATATGGA AGATGAGCAT 
    GTCAAAGAAG GGCATTCTGA TTCTGAAGAC ATTATGTTTG GTGAGGCAGA TACAGACTTG 
    CGGGCTCTTC AGGCCTCGAT GGAACAGTTA CTTAGGGAGC AGCCTAGTGA GGAATTTAGT 
    GAAGAGGAGT TGTCTACCTT AAAGGCAGAT ACTGCAGCAC GCATAACAAA TGGTACAGAG 
    TTGGATGATG AGGATGACAA CAACCCCAGC AGTGAAAGTG TACTTAATGA AGAATGGCAA 
    TCAGACAACA GTGATGGGGA TATTGCCAGT GAATGTGAAG CATATGACAG TGTCTTCAGC 
    CATTTGGAAG AATTGAGATA CAATCTGGAG CGGGAAATAG GCTTTGATAA ATTCATTGAA 
    GTTTATGATA AAATAAAGGC AATACATGAA GATGAAGATG AGAATATTGA TGTTTGTTCA 
    ACAATAGTTC AGAATATTCT GGGAAATGAA TATAAACATC TGTATGCCAA GATTCTTCAT 
    TTAGTGATGG CAGATGGAGC CTATCAGGAG GATAATGATG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829318.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685119.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OCh375629
    Clone ID Related Accession (Same CDS sequence) XM_027829318.1
    Accession Version XM_027829318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GAGGAGTTCC ACCTGGAGTA 
    CGTCCAGGAT TTCCTAATGG GCCTGATGGT CATCACCATT TACCAGATGC TGATGCAATT 
    AGGAAAAAAA TCAAAAGATT GAAGGAGGTG TCTAAACAAG CCAATGCAAA CAGGCAAAGA 
    GGATGGGTAG CAGCAGAAAG AGCAAAACAA GTTGAAGAAT TCTGGCAACG AAAGAGGGAA 
    GCTATGCAAA ACAAAGCTCG TGCTGAAGGA CATATGGGTA CACTGCAAAA CCTGGCAGCT 
    ATCTATGGAG GCAGGCCCAT TTCTGCAAGA GGAAGGAAAT CCAGAAACAA GGAGGAAGAG 
    GAATACCTGG CAAGATTAAG ACAGATCCGA CTTCAAAACT TCAATGAACG CCAGCAGATT 
    AAAGCTAAAC TTCGTGGAGA AAAGAATGAA GCTGACGGTT CTGATGGACA AGAAGGAAGT 
    GAAGAAGCAG AATTGAGACG CAAAAAAATT GAAGCACTAA AGGCCCAAGC AAACGCGCGA 
    GCTGCAGTTC TGAAAGAGCA ACTAGAGCGA AAGAGGAAGG AGGCATATGA AAGAGAGAAG 
    AAAGCTTGGG AAGAACATTT GACAGCAAAA GGGGTAAAGA ATCCTATGGT GCCTTTTAAT 
    GTGTCAGCAG CTGGGGTTAA TCCTGTCCCT GGGCAACAAG AACCTGGAGG ACCTCCTGCC 
    AAACTACAAC TTCCATTGAA GCCCAGTACA CCACTTATAT CTATGACTTC AGCTTTGAAG 
    GAAGTGGGTG TGGATGAAAA TACAACTGCT ATCCAGGAAA CCACAGAGGA AACGAAGAAG 
    GCAGATCTTG CTGTGCAAAA TAAAAGAGAA ATACTAAGAA GATTGAATCA AAATCTTAAA 
    ACACAGGACG ATGTAAAAGG AATAAAGGAG CCTAAAGACA CCTGTGAGGC AGCTATGGCT 
    GAAGAGAGTA AAGAAGAAGA ATATCCACTT CTAGTAGATC GCAAGAAATG GGAGGTGGCA 
    GCAGCTGAGT TGGTGGTTCC TCTTGCACAG CTAACTATGG AGGAATCATT GTCGGGGACA 
    GAGAGACAGA CCGTGGGGGA GGTAATTAAA CTAGATGTTG GTGAGCCCCA GAGAAAAGTT 
    TGGGGCAAAA GCCCTACTGA TTCAGTTTTG AAGATACTGG GGGAAGCAGA GCTACAGCTT 
    CAAACTGAAT TATTGGAGGA AATAATTATC ACAAATGATG TTCCTGAAGA AGGTTCTCAG 
    CCCTTAACCG TGAAAGAGAA CAGTGAAGTA GAAATTGCTT GTATTGGAGA GAAATCTTCA 
    TCTCCAGTTG TTGGTGTCAT AGAATCTGAA AGGACAATAC CAGAGGAGTC ACAAACATTT 
    GATGCCACCC AAGTGCTGTC CAGGCCTGTT GTGCTACATG AGCTGTATGA TTTGGAAGCA 
    GAAATGTTGG AAGAAACTGA AAAAAATAAA AAGTGCAAAA GTGACCAACT TCTCATCACT 
    GTTCATGATG TGTGGGTTAA AGAAAGGGAA ACAATAACGG AGCATGAGCC ACTGGAAGAT 
    ACTGCCTGTC AGAAAGATGG TGGTGGTGAT GAAGCAGCTT TGGAGAAGGA AATGCCGCAA 
    AATGTGCAAC CGCGTAATGA AGTTTCTGCT GGTCATGCTT ACACTTGTCT CAGTGACTCT 
    ATGCAACCTG AACCCTTTTT CCAGAAGGTG GCACAGCCAC AAGCCATACC AGCAGCCTCA 
    CCAGTTCTGT CAGCTCAGTC TTCACTGGAA GAGCCTTTTC CACCTCGTTC ACGTTCTGTA 
    TCACCAGCAA AAAGTAAAGG CAAAAGTTCA TTATTGATTG GGCTCTCTAC TGGCCTGTTT 
    GATGCAAACA ATCCAAAGAT GTTAAGAACA TGTTCACTCC CAGATCTTTC CAAGTTGTAC 
    AGAACACTAA TTGACGTTCC CATTGTAGCA GATGTACAAC ATCAAGACAA TCTTGAAATA 
    GAGGATATGG AAGATGAGCA TGTCAAAGAA GGGCATTCTG ATTCTGAAGA CATTATGTTT 
    GGTGAGGCAG ATACAGACTT GCGGGCTCTT CAGGCCTCGA TGGAACAGTT ACTTAGGGAG 
    CAGCCTAGTG AGGAATTTAG TGAAGAGGAG TTGTCTACCT TAAAGGCAGA TACTGCAGCA 
    CGCATAACAA ATGGTACAGA GTTGGATGAT GAGGATGACA ACAACCCCAG CAGTGAAAGT 
    GTACTTAATG AAGAATGGCA ATCAGACAAC AGTGATGGGG ATATTGCCAG TGAATGTGAA 
    GCATATGACA GTGTCTTCAG CCATTTGGAA GAATTGAGAT ACAATCTGGA GCGGGAAATA 
    GGCTTTGATA AATTCATTGA AGTTTATGAT AAAATAAAGG CAATACATGA AGATGAAGAT 
    GAGAATATTG ATGTTTGTTC AACAATAGTT CAGAATATTC TGGGAAATGA ATATAAACAT 
    CTGTATGCCA AGATTCTTCA TTTAGTGATG GCAGATGGAG CCTATCAGGA GGATAATGAT 
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685119.1
    CDS321..3926
    Translation

    Target ORF information:

    RefSeq Version XM_027829318.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GAGGAGTTCC ACCTGGAGTA 
    CGTCCAGGAT TTCCTAATGG GCCTGATGGT CATCACCATT TACCAGATGC TGATGCAATT 
    AGGAAAAAAA TCAAAAGATT GAAGGAGGTG TCTAAACAAG CCAATGCAAA CAGGCAAAGA 
    GGATGGGTAG CAGCAGAAAG AGCAAAACAA GTTGAAGAAT TCTGGCAACG AAAGAGGGAA 
    GCTATGCAAA ACAAAGCTCG TGCTGAAGGA CATATGGGTA CACTGCAAAA CCTGGCAGCT 
    ATCTATGGAG GCAGGCCCAT TTCTGCAAGA GGAAGGAAAT CCAGAAACAA GGAGGAAGAG 
    GAATACCTGG CAAGATTAAG ACAGATCCGA CTTCAAAACT TCAATGAACG CCAGCAGATT 
    AAAGCTAAAC TTCGTGGAGA AAAGAATGAA GCTGACGGTT CTGATGGACA AGAAGGAAGT 
    GAAGAAGCAG AATTGAGACG CAAAAAAATT GAAGCACTAA AGGCCCAAGC AAACGCGCGA 
    GCTGCAGTTC TGAAAGAGCA ACTAGAGCGA AAGAGGAAGG AGGCATATGA AAGAGAGAAG 
    AAAGCTTGGG AAGAACATTT GACAGCAAAA GGGGTAAAGA ATCCTATGGT GCCTTTTAAT 
    GTGTCAGCAG CTGGGGTTAA TCCTGTCCCT GGGCAACAAG AACCTGGAGG ACCTCCTGCC 
    AAACTACAAC TTCCATTGAA GCCCAGTACA CCACTTATAT CTATGACTTC AGCTTTGAAG 
    GAAGTGGGTG TGGATGAAAA TACAACTGCT ATCCAGGAAA CCACAGAGGA AACGAAGAAG 
    GCAGATCTTG CTGTGCAAAA TAAAAGAGAA ATACTAAGAA GATTGAATCA AAATCTTAAA 
    ACACAGGACG ATGTAAAAGG AATAAAGGAG CCTAAAGACA CCTGTGAGGC AGCTATGGCT 
    GAAGAGAGTA AAGAAGAAGA ATATCCACTT CTAGTAGATC GCAAGAAATG GGAGGTGGCA 
    GCAGCTGAGT TGGTGGTTCC TCTTGCACAG CTAACTATGG AGGAATCATT GTCGGGGACA 
    GAGAGACAGA CCGTGGGGGA GGTAATTAAA CTAGATGTTG GTGAGCCCCA GAGAAAAGTT 
    TGGGGCAAAA GCCCTACTGA TTCAGTTTTG AAGATACTGG GGGAAGCAGA GCTACAGCTT 
    CAAACTGAAT TATTGGAGGA AATAATTATC ACAAATGATG TTCCTGAAGA AGGTTCTCAG 
    CCCTTAACCG TGAAAGAGAA CAGTGAAGTA GAAATTGCTT GTATTGGAGA GAAATCTTCA 
    TCTCCAGTTG TTGGTGTCAT AGAATCTGAA AGGACAATAC CAGAGGAGTC ACAAACATTT 
    GATGCCACCC AAGTGCTGTC CAGGCCTGTT GTGCTACATG AGCTGTATGA TTTGGAAGCA 
    GAAATGTTGG AAGAAACTGA AAAAAATAAA AAGTGCAAAA GTGACCAACT TCTCATCACT 
    GTTCATGATG TGTGGGTTAA AGAAAGGGAA ACAATAACGG AGCATGAGCC ACTGGAAGAT 
    ACTGCCTGTC AGAAAGATGG TGGTGGTGAT GAAGCAGCTT TGGAGAAGGA AATGCCGCAA 
    AATGTGCAAC CGCGTAATGA AGTTTCTGCT GGTCATGCTT ACACTTGTCT CAGTGACTCT 
    ATGCAACCTG AACCCTTTTT CCAGAAGGTG GCACAGCCAC AAGCCATACC AGCAGCCTCA 
    CCAGTTCTGT CAGCTCAGTC TTCACTGGAA GAGCCTTTTC CACCTCGTTC ACGTTCTGTA 
    TCACCAGCAA AAAGTAAAGG CAAAAGTTCA TTATTGATTG GGCTCTCTAC TGGCCTGTTT 
    GATGCAAACA ATCCAAAGAT GTTAAGAACA TGTTCACTCC CAGATCTTTC CAAGTTGTAC 
    AGAACACTAA TTGACGTTCC CATTGTAGCA GATGTACAAC ATCAAGACAA TCTTGAAATA 
    GAGGATATGG AAGATGAGCA TGTCAAAGAA GGGCATTCTG ATTCTGAAGA CATTATGTTT 
    GGTGAGGCAG ATACAGACTT GCGGGCTCTT CAGGCCTCGA TGGAACAGTT ACTTAGGGAG 
    CAGCCTAGTG AGGAATTTAG TGAAGAGGAG TTGTCTACCT TAAAGGCAGA TACTGCAGCA 
    CGCATAACAA ATGGTACAGA GTTGGATGAT GAGGATGACA ACAACCCCAG CAGTGAAAGT 
    GTACTTAATG AAGAATGGCA ATCAGACAAC AGTGATGGGG ATATTGCCAG TGAATGTGAA 
    GCATATGACA GTGTCTTCAG CCATTTGGAA GAATTGAGAT ACAATCTGGA GCGGGAAATA 
    GGCTTTGATA AATTCATTGA AGTTTATGAT AAAATAAAGG CAATACATGA AGATGAAGAT 
    GAGAATATTG ATGTTTGTTC AACAATAGTT CAGAATATTC TGGGAAATGA ATATAAACAT 
    CTGTATGCCA AGATTCTTCA TTTAGTGATG GCAGATGGAG CCTATCAGGA GGATAATGAT 
    GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829319.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685120.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OCh375630
    Clone ID Related Accession (Same CDS sequence) XM_027829319.1
    Accession Version XM_027829319.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GAGGAGTTCC ACCTGGAGTA 
    CGTCCAGGAT TTCCTAATGG GCCTGATGGT CATCACCATT TACCAGATGC TGATGCAATT 
    AGGAAAAAAA TCAAAAGATT GAAGGAGGTG TCTAAACAAG CCAATGCAAA CAGGCAAAGA 
    GGATGGGTAG CAGCAGAAAG AGCAAAACAA GTTGAAGAAT TCTGGCAACG AAAGAGGGAA 
    GCTATGCAAA ACAAAGCTCG TGCTGAAGGA CATATGGAAT ACCTGGCAAG ATTAAGACAG 
    ATCCGACTTC AAAACTTCAA TGAACGCCAG CAGATTAAAG CTAAACTTCG TGGAGAAAAG 
    AATGAAGCTG ACGGTTCTGA TGGACAAGAA GGAAGTGAAG AAGCAGAATT GAGACGCAAA 
    AAAATTGAAG CACTAAAGGC CCAAGCAAAC GCGCGAGCTG CAGTTCTGAA AGAGCAACTA 
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAGAAAG CTTGGGAAGA ACATTTGACA 
    GCAAAAGGGG TAAAGAATCC TATGGTGCCT TTTAATGTGT CAGCAGCTGG GGTTAATCCT 
    GTCCCTGGGC AACAAGAACC TGGAGGACCT CCTGCCAAAC TACAACTTCC ATTGAAGCCC 
    AGTACACCAC TTATATCTAT GACTTCAGCT TTGAAGGAAG TGGGTGTGGA TGAAAATACA 
    ACTGCTATCC AGGAAACCAC AGAGGAAACG AAGAAGGCAG ATCTTGCTGT GCAAAATAAA 
    AGAGAAATAC TAAGAAGATT GAATCAAAAT CTTAAAACAC AGGACGATGT AAAAGGAATA 
    AAGGAGCCTA AAGACACCTG TGAGGCAGCT ATGGCTGAAG AGAGTAAAGA AGAAGAATAT 
    CCACTTCTAG TAGATCGCAA GAAATGGGAG GTGGCAGCAG CTGAGTTGGT GGTTCCTCTT 
    GCACAGCTAA CTATGGAGGA ATCATTGTCG GGGACAGAGA GACAGACCGT GGGGGAGGTA 
    ATTAAACTAG ATGTTGGTGA GCCCCAGAGA AAAGTTTGGG GCAAAAGCCC TACTGATTCA 
    GTTTTGAAGA TACTGGGGGA AGCAGAGCTA CAGCTTCAAA CTGAATTATT GGAGGAAATA 
    ATTATCACAA ATGATGTTCC TGAAGAAGGT TCTCAGCCCT TAACCGTGAA AGAGAACAGT 
    GAAGTAGAAA TTGCTTGTAT TGGAGAGAAA TCTTCATCTC CAGTTGTTGG TGTCATAGAA 
    TCTGAAAGGA CAATACCAGA GGAGTCACAA ACATTTGATG CCACCCAAGT GCTGTCCAGG 
    CCTGTTGTGC TACATGAGCT GTATGATTTG GAAGCAGAAA TGTTGGAAGA AACTGAAAAA 
    AATAAAAAGT GCAAAAGTGA CCAACTTCTC ATCACTGTTC ATGATGTGTG GGTTAAAGAA 
    AGGGAAACAA TAACGGAGCA TGAGCCACTG GAAGATACTG CCTGTCAGAA AGATGGTGGT 
    GGTGATGAAG CAGCTTTGGA GAAGGAAATG CCGCAAAATG TGCAACCGCG TAATGAAGTT 
    TCTGCTGGTC ATGCTTACAC TTGTCTCAGT GACTCTATGC AACCTGAACC CTTTTTCCAG 
    AAGGTGGCAC AGCCACAAGC CATACCAGCA GCCTCACCAG TTCTGTCAGC TCAGTCTTCA 
    CTGGAAGAGC CTTTTCCACC TCGTTCACGT TCTGTATCAC CAGCAAAAAG TAAAGGCAAA 
    AGTTCATTAT TGATTGGGCT CTCTACTGGC CTGTTTGATG CAAACAATCC AAAGATGTTA 
    AGAACATGTT CACTCCCAGA TCTTTCCAAG TTGTACAGAA CACTAATTGA CGTTCCCATT 
    GTAGCAGATG TACAACATCA AGACAATCTT GAAATAGAGG ATATGGAAGA TGAGCATGTC 
    AAAGAAGGGC ATTCTGATTC TGAAGACATT ATGTTTGGTG AGGCAGATAC AGACTTGCGG 
    GCTCTTCAGG CCTCGATGGA ACAGTTACTT AGGGAGCAGC CTAGTGAGGA ATTTAGTGAA 
    GAGGAGTTGT CTACCTTAAA GGCAGATACT GCAGCACGCA TAACAAATGG TACAGAGTTG 
    GATGATGAGG ATGACAACAA CCCCAGCAGT GAAAGTGTAC TTAATGAAGA ATGGCAATCA 
    GACAACAGTG ATGGGGATAT TGCCAGTGAA TGTGAAGCAT ATGACAGTGT CTTCAGCCAT 
    TTGGAAGAAT TGAGATACAA TCTGGAGCGG GAAATAGGCT TTGATAAATT CATTGAAGTT 
    TATGATAAAA TAAAGGCAAT ACATGAAGAT GAAGATGAGA ATATTGATGT TTGTTCAACA 
    ATAGTTCAGA ATATTCTGGG AAATGAATAT AAACATCTGT ATGCCAAGAT TCTTCATTTA 
    GTGATGGCAG ATGGAGCCTA TCAGGAGGAT AATGATGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685120.1
    CDS321..3842
    Translation

    Target ORF information:

    RefSeq Version XM_027829319.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829319.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GAGGAGTTCC ACCTGGAGTA 
    CGTCCAGGAT TTCCTAATGG GCCTGATGGT CATCACCATT TACCAGATGC TGATGCAATT 
    AGGAAAAAAA TCAAAAGATT GAAGGAGGTG TCTAAACAAG CCAATGCAAA CAGGCAAAGA 
    GGATGGGTAG CAGCAGAAAG AGCAAAACAA GTTGAAGAAT TCTGGCAACG AAAGAGGGAA 
    GCTATGCAAA ACAAAGCTCG TGCTGAAGGA CATATGGAAT ACCTGGCAAG ATTAAGACAG 
    ATCCGACTTC AAAACTTCAA TGAACGCCAG CAGATTAAAG CTAAACTTCG TGGAGAAAAG 
    AATGAAGCTG ACGGTTCTGA TGGACAAGAA GGAAGTGAAG AAGCAGAATT GAGACGCAAA 
    AAAATTGAAG CACTAAAGGC CCAAGCAAAC GCGCGAGCTG CAGTTCTGAA AGAGCAACTA 
    GAGCGAAAGA GGAAGGAGGC ATATGAAAGA GAGAAGAAAG CTTGGGAAGA ACATTTGACA 
    GCAAAAGGGG TAAAGAATCC TATGGTGCCT TTTAATGTGT CAGCAGCTGG GGTTAATCCT 
    GTCCCTGGGC AACAAGAACC TGGAGGACCT CCTGCCAAAC TACAACTTCC ATTGAAGCCC 
    AGTACACCAC TTATATCTAT GACTTCAGCT TTGAAGGAAG TGGGTGTGGA TGAAAATACA 
    ACTGCTATCC AGGAAACCAC AGAGGAAACG AAGAAGGCAG ATCTTGCTGT GCAAAATAAA 
    AGAGAAATAC TAAGAAGATT GAATCAAAAT CTTAAAACAC AGGACGATGT AAAAGGAATA 
    AAGGAGCCTA AAGACACCTG TGAGGCAGCT ATGGCTGAAG AGAGTAAAGA AGAAGAATAT 
    CCACTTCTAG TAGATCGCAA GAAATGGGAG GTGGCAGCAG CTGAGTTGGT GGTTCCTCTT 
    GCACAGCTAA CTATGGAGGA ATCATTGTCG GGGACAGAGA GACAGACCGT GGGGGAGGTA 
    ATTAAACTAG ATGTTGGTGA GCCCCAGAGA AAAGTTTGGG GCAAAAGCCC TACTGATTCA 
    GTTTTGAAGA TACTGGGGGA AGCAGAGCTA CAGCTTCAAA CTGAATTATT GGAGGAAATA 
    ATTATCACAA ATGATGTTCC TGAAGAAGGT TCTCAGCCCT TAACCGTGAA AGAGAACAGT 
    GAAGTAGAAA TTGCTTGTAT TGGAGAGAAA TCTTCATCTC CAGTTGTTGG TGTCATAGAA 
    TCTGAAAGGA CAATACCAGA GGAGTCACAA ACATTTGATG CCACCCAAGT GCTGTCCAGG 
    CCTGTTGTGC TACATGAGCT GTATGATTTG GAAGCAGAAA TGTTGGAAGA AACTGAAAAA 
    AATAAAAAGT GCAAAAGTGA CCAACTTCTC ATCACTGTTC ATGATGTGTG GGTTAAAGAA 
    AGGGAAACAA TAACGGAGCA TGAGCCACTG GAAGATACTG CCTGTCAGAA AGATGGTGGT 
    GGTGATGAAG CAGCTTTGGA GAAGGAAATG CCGCAAAATG TGCAACCGCG TAATGAAGTT 
    TCTGCTGGTC ATGCTTACAC TTGTCTCAGT GACTCTATGC AACCTGAACC CTTTTTCCAG 
    AAGGTGGCAC AGCCACAAGC CATACCAGCA GCCTCACCAG TTCTGTCAGC TCAGTCTTCA 
    CTGGAAGAGC CTTTTCCACC TCGTTCACGT TCTGTATCAC CAGCAAAAAG TAAAGGCAAA 
    AGTTCATTAT TGATTGGGCT CTCTACTGGC CTGTTTGATG CAAACAATCC AAAGATGTTA 
    AGAACATGTT CACTCCCAGA TCTTTCCAAG TTGTACAGAA CACTAATTGA CGTTCCCATT 
    GTAGCAGATG TACAACATCA AGACAATCTT GAAATAGAGG ATATGGAAGA TGAGCATGTC 
    AAAGAAGGGC ATTCTGATTC TGAAGACATT ATGTTTGGTG AGGCAGATAC AGACTTGCGG 
    GCTCTTCAGG CCTCGATGGA ACAGTTACTT AGGGAGCAGC CTAGTGAGGA ATTTAGTGAA 
    GAGGAGTTGT CTACCTTAAA GGCAGATACT GCAGCACGCA TAACAAATGG TACAGAGTTG 
    GATGATGAGG ATGACAACAA CCCCAGCAGT GAAAGTGTAC TTAATGAAGA ATGGCAATCA 
    GACAACAGTG ATGGGGATAT TGCCAGTGAA TGTGAAGCAT ATGACAGTGT CTTCAGCCAT 
    TTGGAAGAAT TGAGATACAA TCTGGAGCGG GAAATAGGCT TTGATAAATT CATTGAAGTT 
    TATGATAAAA TAAAGGCAAT ACATGAAGAT GAAGATGAGA ATATTGATGT TTGTTCAACA 
    ATAGTTCAGA ATATTCTGGG AAATGAATAT AAACATCTGT ATGCCAAGAT TCTTCATTTA 
    GTGATGGCAG ATGGAGCCTA TCAGGAGGAT AATGATGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829320.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685121.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID OCh375631
    Clone ID Related Accession (Same CDS sequence) XM_027829320.1
    Accession Version XM_027829320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GGCAAAGAGG ATGGGTAGCA 
    GCAGAAAGAG CAAAACAAGT TGAAGAATTC TGGCAACGAA AGAGGGAAGC TATGCAAAAC 
    AAAGCTCGTG CTGAAGGACA TATGGGTACA CTGCAAAACC TGGCAGCTAT CTATGGAGGC 
    AGGCCCATTT CTGCAAGAGG AAGGAAATCC AGAAACAAGG AGGAAGAGGA ATACCTGGCA 
    AGATTAAGAC AGATCCGACT TCAAAACTTC AATGAACGCC AGCAGATTAA AGCTAAACTT 
    CGTGGAGAAA AGAATGAAGC TGACGGTTCT GATGGACAAG AAGGAAGTGA AGAAGCAGAA 
    TTGAGACGCA AAAAAATTGA AGCACTAAAG GCCCAAGCAA ACGCGCGAGC TGCAGTTCTG 
    AAAGAGCAAC TAGAGCGAAA GAGGAAGGAG GCATATGAAA GAGAGAAGAA AGCTTGGGAA 
    GAACATTTGA CAGCAAAAGG GGTAAAGAAT CCTATGGTGC CTTTTAATGT GTCAGCAGCT 
    GGGGTTAATC CTGTCCCTGG GCAACAAGAA CCTGGAGGAC CTCCTGCCAA ACTACAACTT 
    CCATTGAAGC CCAGTACACC ACTTATATCT ATGACTTCAG CTTTGAAGGA AGTGGGTGTG 
    GATGAAAATA CAACTGCTAT CCAGGAAACC ACAGAGGAAA CGAAGAAGGC AGATCTTGCT 
    GTGCAAAATA AAAGAGAAAT ACTAAGAAGA TTGAATCAAA ATCTTAAAAC ACAGGACGAT 
    GTAAAAGGAA TAAAGGAGCC TAAAGACACC TGTGAGGCAG CTATGGCTGA AGAGAGTAAA 
    GAAGAAGAAT ATCCACTTCT AGTAGATCGC AAGAAATGGG AGGTGGCAGC AGCTGAGTTG 
    GTGGTTCCTC TTGCACAGCT AACTATGGAG GAATCATTGT CGGGGACAGA GAGACAGACC 
    GTGGGGGAGG TAATTAAACT AGATGTTGGT GAGCCCCAGA GAAAAGTTTG GGGCAAAAGC 
    CCTACTGATT CAGTTTTGAA GATACTGGGG GAAGCAGAGC TACAGCTTCA AACTGAATTA 
    TTGGAGGAAA TAATTATCAC AAATGATGTT CCTGAAGAAG GTTCTCAGCC CTTAACCGTG 
    AAAGAGAACA GTGAAGTAGA AATTGCTTGT ATTGGAGAGA AATCTTCATC TCCAGTTGTT 
    GGTGTCATAG AATCTGAAAG GACAATACCA GAGGAGTCAC AAACATTTGA TGCCACCCAA 
    GTGCTGTCCA GGCCTGTTGT GCTACATGAG CTGTATGATT TGGAAGCAGA AATGTTGGAA 
    GAAACTGAAA AAAATAAAAA GTGCAAAAGT GACCAACTTC TCATCACTGT TCATGATGTG 
    TGGGTTAAAG AAAGGGAAAC AATAACGGAG CATGAGCCAC TGGAAGATAC TGCCTGTCAG 
    AAAGATGGTG GTGGTGATGA AGCAGCTTTG GAGAAGGAAA TGCCGCAAAA TGTGCAACCG 
    CGTAATGAAG TTTCTGCTGG TCATGCTTAC ACTTGTCTCA GTGACTCTAT GCAACCTGAA 
    CCCTTTTTCC AGAAGGTGGC ACAGCCACAA GCCATACCAG CAGCCTCACC AGTTCTGTCA 
    GCTCAGTCTT CACTGGAAGA GCCTTTTCCA CCTCGTTCAC GTTCTGTATC ACCAGCAAAA 
    AGTAAAGGCA AAAGTTCATT ATTGATTGGG CTCTCTACTG GCCTGTTTGA TGCAAACAAT 
    CCAAAGATGT TAAGAACATG TTCACTCCCA GATCTTTCCA AGTTGTACAG AACACTAATT 
    GACGTTCCCA TTGTAGCAGA TGTACAACAT CAAGACAATC TTGAAATAGA GGATATGGAA 
    GATGAGCATG TCAAAGAAGG GCATTCTGAT TCTGAAGACA TTATGTTTGG TGAGGCAGAT 
    ACAGACTTGC GGGCTCTTCA GGCCTCGATG GAACAGTTAC TTAGGGAGCA GCCTAGTGAG 
    GAATTTAGTG AAGAGGAGTT GTCTACCTTA AAGGCAGATA CTGCAGCACG CATAACAAAT 
    GGTACAGAGT TGGATGATGA GGATGACAAC AACCCCAGCA GTGAAAGTGT ACTTAATGAA 
    GAATGGCAAT CAGACAACAG TGATGGGGAT ATTGCCAGTG AATGTGAAGC ATATGACAGT 
    GTCTTCAGCC ATTTGGAAGA ATTGAGATAC AATCTGGAGC GGGAAATAGG CTTTGATAAA 
    TTCATTGAAG TTTATGATAA AATAAAGGCA ATACATGAAG ATGAAGATGA GAATATTGAT 
    GTTTGTTCAA CAATAGTTCA GAATATTCTG GGAAATGAAT ATAAACATCT GTATGCCAAG 
    ATTCTTCATT TAGTGATGGC AGATGGAGCC TATCAGGAGG ATAATGATGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685121.1
    CDS321..3794
    Translation

    Target ORF information:

    RefSeq Version XM_027829320.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GGCAAAGAGG ATGGGTAGCA 
    GCAGAAAGAG CAAAACAAGT TGAAGAATTC TGGCAACGAA AGAGGGAAGC TATGCAAAAC 
    AAAGCTCGTG CTGAAGGACA TATGGGTACA CTGCAAAACC TGGCAGCTAT CTATGGAGGC 
    AGGCCCATTT CTGCAAGAGG AAGGAAATCC AGAAACAAGG AGGAAGAGGA ATACCTGGCA 
    AGATTAAGAC AGATCCGACT TCAAAACTTC AATGAACGCC AGCAGATTAA AGCTAAACTT 
    CGTGGAGAAA AGAATGAAGC TGACGGTTCT GATGGACAAG AAGGAAGTGA AGAAGCAGAA 
    TTGAGACGCA AAAAAATTGA AGCACTAAAG GCCCAAGCAA ACGCGCGAGC TGCAGTTCTG 
    AAAGAGCAAC TAGAGCGAAA GAGGAAGGAG GCATATGAAA GAGAGAAGAA AGCTTGGGAA 
    GAACATTTGA CAGCAAAAGG GGTAAAGAAT CCTATGGTGC CTTTTAATGT GTCAGCAGCT 
    GGGGTTAATC CTGTCCCTGG GCAACAAGAA CCTGGAGGAC CTCCTGCCAA ACTACAACTT 
    CCATTGAAGC CCAGTACACC ACTTATATCT ATGACTTCAG CTTTGAAGGA AGTGGGTGTG 
    GATGAAAATA CAACTGCTAT CCAGGAAACC ACAGAGGAAA CGAAGAAGGC AGATCTTGCT 
    GTGCAAAATA AAAGAGAAAT ACTAAGAAGA TTGAATCAAA ATCTTAAAAC ACAGGACGAT 
    GTAAAAGGAA TAAAGGAGCC TAAAGACACC TGTGAGGCAG CTATGGCTGA AGAGAGTAAA 
    GAAGAAGAAT ATCCACTTCT AGTAGATCGC AAGAAATGGG AGGTGGCAGC AGCTGAGTTG 
    GTGGTTCCTC TTGCACAGCT AACTATGGAG GAATCATTGT CGGGGACAGA GAGACAGACC 
    GTGGGGGAGG TAATTAAACT AGATGTTGGT GAGCCCCAGA GAAAAGTTTG GGGCAAAAGC 
    CCTACTGATT CAGTTTTGAA GATACTGGGG GAAGCAGAGC TACAGCTTCA AACTGAATTA 
    TTGGAGGAAA TAATTATCAC AAATGATGTT CCTGAAGAAG GTTCTCAGCC CTTAACCGTG 
    AAAGAGAACA GTGAAGTAGA AATTGCTTGT ATTGGAGAGA AATCTTCATC TCCAGTTGTT 
    GGTGTCATAG AATCTGAAAG GACAATACCA GAGGAGTCAC AAACATTTGA TGCCACCCAA 
    GTGCTGTCCA GGCCTGTTGT GCTACATGAG CTGTATGATT TGGAAGCAGA AATGTTGGAA 
    GAAACTGAAA AAAATAAAAA GTGCAAAAGT GACCAACTTC TCATCACTGT TCATGATGTG 
    TGGGTTAAAG AAAGGGAAAC AATAACGGAG CATGAGCCAC TGGAAGATAC TGCCTGTCAG 
    AAAGATGGTG GTGGTGATGA AGCAGCTTTG GAGAAGGAAA TGCCGCAAAA TGTGCAACCG 
    CGTAATGAAG TTTCTGCTGG TCATGCTTAC ACTTGTCTCA GTGACTCTAT GCAACCTGAA 
    CCCTTTTTCC AGAAGGTGGC ACAGCCACAA GCCATACCAG CAGCCTCACC AGTTCTGTCA 
    GCTCAGTCTT CACTGGAAGA GCCTTTTCCA CCTCGTTCAC GTTCTGTATC ACCAGCAAAA 
    AGTAAAGGCA AAAGTTCATT ATTGATTGGG CTCTCTACTG GCCTGTTTGA TGCAAACAAT 
    CCAAAGATGT TAAGAACATG TTCACTCCCA GATCTTTCCA AGTTGTACAG AACACTAATT 
    GACGTTCCCA TTGTAGCAGA TGTACAACAT CAAGACAATC TTGAAATAGA GGATATGGAA 
    GATGAGCATG TCAAAGAAGG GCATTCTGAT TCTGAAGACA TTATGTTTGG TGAGGCAGAT 
    ACAGACTTGC GGGCTCTTCA GGCCTCGATG GAACAGTTAC TTAGGGAGCA GCCTAGTGAG 
    GAATTTAGTG AAGAGGAGTT GTCTACCTTA AAGGCAGATA CTGCAGCACG CATAACAAAT 
    GGTACAGAGT TGGATGATGA GGATGACAAC AACCCCAGCA GTGAAAGTGT ACTTAATGAA 
    GAATGGCAAT CAGACAACAG TGATGGGGAT ATTGCCAGTG AATGTGAAGC ATATGACAGT 
    GTCTTCAGCC ATTTGGAAGA ATTGAGATAC AATCTGGAGC GGGAAATAGG CTTTGATAAA 
    TTCATTGAAG TTTATGATAA AATAAAGGCA ATACATGAAG ATGAAGATGA GAATATTGAT 
    GTTTGTTCAA CAATAGTTCA GAATATTCTG GGAAATGAAT ATAAACATCT GTATGCCAAG 
    ATTCTTCATT TAGTGATGGC AGATGGAGCC TATCAGGAGG ATAATGATGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_027829321.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_027685122.1 Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OCh375632
    Clone ID Related Accession (Same CDS sequence) XM_027829321.1
    Accession Version XM_027829321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1548086400000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Chelonia mydas Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GGCAAAGAGG ATGGGTAGCA 
    GCAGAAAGAG CAAAACAAGT TGAAGAATTC TGGCAACGAA AGAGGGAAGC TATGCAAAAC 
    AAAGCTCGTG CTGAAGGACA TATGGAATAC CTGGCAAGAT TAAGACAGAT CCGACTTCAA 
    AACTTCAATG AACGCCAGCA GATTAAAGCT AAACTTCGTG GAGAAAAGAA TGAAGCTGAC 
    GGTTCTGATG GACAAGAAGG AAGTGAAGAA GCAGAATTGA GACGCAAAAA AATTGAAGCA 
    CTAAAGGCCC AAGCAAACGC GCGAGCTGCA GTTCTGAAAG AGCAACTAGA GCGAAAGAGG 
    AAGGAGGCAT ATGAAAGAGA GAAGAAAGCT TGGGAAGAAC ATTTGACAGC AAAAGGGGTA 
    AAGAATCCTA TGGTGCCTTT TAATGTGTCA GCAGCTGGGG TTAATCCTGT CCCTGGGCAA 
    CAAGAACCTG GAGGACCTCC TGCCAAACTA CAACTTCCAT TGAAGCCCAG TACACCACTT 
    ATATCTATGA CTTCAGCTTT GAAGGAAGTG GGTGTGGATG AAAATACAAC TGCTATCCAG 
    GAAACCACAG AGGAAACGAA GAAGGCAGAT CTTGCTGTGC AAAATAAAAG AGAAATACTA 
    AGAAGATTGA ATCAAAATCT TAAAACACAG GACGATGTAA AAGGAATAAA GGAGCCTAAA 
    GACACCTGTG AGGCAGCTAT GGCTGAAGAG AGTAAAGAAG AAGAATATCC ACTTCTAGTA 
    GATCGCAAGA AATGGGAGGT GGCAGCAGCT GAGTTGGTGG TTCCTCTTGC ACAGCTAACT 
    ATGGAGGAAT CATTGTCGGG GACAGAGAGA CAGACCGTGG GGGAGGTAAT TAAACTAGAT 
    GTTGGTGAGC CCCAGAGAAA AGTTTGGGGC AAAAGCCCTA CTGATTCAGT TTTGAAGATA 
    CTGGGGGAAG CAGAGCTACA GCTTCAAACT GAATTATTGG AGGAAATAAT TATCACAAAT 
    GATGTTCCTG AAGAAGGTTC TCAGCCCTTA ACCGTGAAAG AGAACAGTGA AGTAGAAATT 
    GCTTGTATTG GAGAGAAATC TTCATCTCCA GTTGTTGGTG TCATAGAATC TGAAAGGACA 
    ATACCAGAGG AGTCACAAAC ATTTGATGCC ACCCAAGTGC TGTCCAGGCC TGTTGTGCTA 
    CATGAGCTGT ATGATTTGGA AGCAGAAATG TTGGAAGAAA CTGAAAAAAA TAAAAAGTGC 
    AAAAGTGACC AACTTCTCAT CACTGTTCAT GATGTGTGGG TTAAAGAAAG GGAAACAATA 
    ACGGAGCATG AGCCACTGGA AGATACTGCC TGTCAGAAAG ATGGTGGTGG TGATGAAGCA 
    GCTTTGGAGA AGGAAATGCC GCAAAATGTG CAACCGCGTA ATGAAGTTTC TGCTGGTCAT 
    GCTTACACTT GTCTCAGTGA CTCTATGCAA CCTGAACCCT TTTTCCAGAA GGTGGCACAG 
    CCACAAGCCA TACCAGCAGC CTCACCAGTT CTGTCAGCTC AGTCTTCACT GGAAGAGCCT 
    TTTCCACCTC GTTCACGTTC TGTATCACCA GCAAAAAGTA AAGGCAAAAG TTCATTATTG 
    ATTGGGCTCT CTACTGGCCT GTTTGATGCA AACAATCCAA AGATGTTAAG AACATGTTCA 
    CTCCCAGATC TTTCCAAGTT GTACAGAACA CTAATTGACG TTCCCATTGT AGCAGATGTA 
    CAACATCAAG ACAATCTTGA AATAGAGGAT ATGGAAGATG AGCATGTCAA AGAAGGGCAT 
    TCTGATTCTG AAGACATTAT GTTTGGTGAG GCAGATACAG ACTTGCGGGC TCTTCAGGCC 
    TCGATGGAAC AGTTACTTAG GGAGCAGCCT AGTGAGGAAT TTAGTGAAGA GGAGTTGTCT 
    ACCTTAAAGG CAGATACTGC AGCACGCATA ACAAATGGTA CAGAGTTGGA TGATGAGGAT 
    GACAACAACC CCAGCAGTGA AAGTGTACTT AATGAAGAAT GGCAATCAGA CAACAGTGAT 
    GGGGATATTG CCAGTGAATG TGAAGCATAT GACAGTGTCT TCAGCCATTT GGAAGAATTG 
    AGATACAATC TGGAGCGGGA AATAGGCTTT GATAAATTCA TTGAAGTTTA TGATAAAATA 
    AAGGCAATAC ATGAAGATGA AGATGAGAAT ATTGATGTTT GTTCAACAAT AGTTCAGAAT 
    ATTCTGGGAA ATGAATATAA ACATCTGTAT GCCAAGATTC TTCATTTAGT GATGGCAGAT 
    GGAGCCTATC AGGAGGATAA TGATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027685122.1
    CDS321..3710
    Translation

    Target ORF information:

    RefSeq Version XM_027829321.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA related kinase 1 (NEK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_027829321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGATTGGT TTGTACAGAT TTGTTTAGCA CTAAAACATG TACATGACAG AAAAATTTTG 
    CATCGAGATA TAAAGTCACA GAATATATTT TTAACCAAAG ATGGAACAAT ACAACTGGGA 
    GACTTTGGAA TAGCTAGAGT TCTTAACAGT ACTGTAGAGC TGGCTCGCAC TTGCATAGGA 
    ACACCATACT ATTTGTCACC TGAAATCTGT CGGAACAAGC CTTACAACAA TAAAAGTGAC 
    ATATGGGCTC TTGGTTGTGT TCTCTATGAG ATGTGCACAC TTAAACATGC ATTTGAAGCT 
    GGTAACATGA AAAATCTGGT ACTGAAGATA ATCTCTGGAT CTTTTCCTCC TGTTTCTCTG 
    CATTATTCCT ACGATCTACG TAATCTGCTT TCACAGTTAT TTAAAAGGAA TCCTAGGGAT 
    AGACCATCAG TCAACTCCAT ATTGGAGAAA ATCTTTATAG CCAAACGGGT GGAAAAATTT 
    CTTACACCAC AGCTTATTGC AGACGAATTC AGTCACAAAG TGTTCCACAA GTTTGACGCC 
    CATGTTGCAC CAGTAAAACG ACTAGCTCAA GGACAAATCT GTGCTTCAGC TGTGCCAGCT 
    CAGAAAATTA CCAAACCTGC TGCAAAATAT GGAGTGCCTT TAATGATCAA GAAATCTGGT 
    GATGCATCTA AAAAATTTCA TGAGAAGAAG CCATTAGCTA AATTTAGACA GGCCCATCCA 
    ACTCCAATGA AGAAAATGAA TCCAGGAGAA GAAAGGAGGA AAATGTTTGA GGAAGCTGCA 
    AAAAAGAGAA GATTTGAATT ACTTGAGAAA GAAAAACGAC AAAGAGACCA GAAGGCTGAG 
    CAGATGAGAA GACTGGAAAA GGAAAGGATG GAACGGATAA ACAGAGCCAG GGAACAAGGC 
    TGGAGAAATG TGCTTAGTTC AGGTGGAAGT GGTGAAGTTA AGGCACCCTT TTTTGGCGGT 
    GGAGGAGGTG TTGGGCCTTC CCCTGTGCCT GCCAGAGGAC AGTATGAACA CTACCATGCT 
    ATCTTTGACC AGATGCAACA GCAAAAGGAA AATATTAAAG TAGGTGAGGA GAGAGAAACC 
    AGATGGAGAG CAGAAATACG AAGTCGGGAA GCTGTTGAAA GGCAAAGAGG ATGGGTAGCA 
    GCAGAAAGAG CAAAACAAGT TGAAGAATTC TGGCAACGAA AGAGGGAAGC TATGCAAAAC 
    AAAGCTCGTG CTGAAGGACA TATGGAATAC CTGGCAAGAT TAAGACAGAT CCGACTTCAA 
    AACTTCAATG AACGCCAGCA GATTAAAGCT AAACTTCGTG GAGAAAAGAA TGAAGCTGAC 
    GGTTCTGATG GACAAGAAGG AAGTGAAGAA GCAGAATTGA GACGCAAAAA AATTGAAGCA 
    CTAAAGGCCC AAGCAAACGC GCGAGCTGCA GTTCTGAAAG AGCAACTAGA GCGAAAGAGG 
    AAGGAGGCAT ATGAAAGAGA GAAGAAAGCT TGGGAAGAAC ATTTGACAGC AAAAGGGGTA 
    AAGAATCCTA TGGTGCCTTT TAATGTGTCA GCAGCTGGGG TTAATCCTGT CCCTGGGCAA 
    CAAGAACCTG GAGGACCTCC TGCCAAACTA CAACTTCCAT TGAAGCCCAG TACACCACTT 
    ATATCTATGA CTTCAGCTTT GAAGGAAGTG GGTGTGGATG AAAATACAAC TGCTATCCAG 
    GAAACCACAG AGGAAACGAA GAAGGCAGAT CTTGCTGTGC AAAATAAAAG AGAAATACTA 
    AGAAGATTGA ATCAAAATCT TAAAACACAG GACGATGTAA AAGGAATAAA GGAGCCTAAA 
    GACACCTGTG AGGCAGCTAT GGCTGAAGAG AGTAAAGAAG AAGAATATCC ACTTCTAGTA 
    GATCGCAAGA AATGGGAGGT GGCAGCAGCT GAGTTGGTGG TTCCTCTTGC ACAGCTAACT 
    ATGGAGGAAT CATTGTCGGG GACAGAGAGA CAGACCGTGG GGGAGGTAAT TAAACTAGAT 
    GTTGGTGAGC CCCAGAGAAA AGTTTGGGGC AAAAGCCCTA CTGATTCAGT TTTGAAGATA 
    CTGGGGGAAG CAGAGCTACA GCTTCAAACT GAATTATTGG AGGAAATAAT TATCACAAAT 
    GATGTTCCTG AAGAAGGTTC TCAGCCCTTA ACCGTGAAAG AGAACAGTGA AGTAGAAATT 
    GCTTGTATTG GAGAGAAATC TTCATCTCCA GTTGTTGGTG TCATAGAATC TGAAAGGACA 
    ATACCAGAGG AGTCACAAAC ATTTGATGCC ACCCAAGTGC TGTCCAGGCC TGTTGTGCTA 
    CATGAGCTGT ATGATTTGGA AGCAGAAATG TTGGAAGAAA CTGAAAAAAA TAAAAAGTGC 
    AAAAGTGACC AACTTCTCAT CACTGTTCAT GATGTGTGGG TTAAAGAAAG GGAAACAATA 
    ACGGAGCATG AGCCACTGGA AGATACTGCC TGTCAGAAAG ATGGTGGTGG TGATGAAGCA 
    GCTTTGGAGA AGGAAATGCC GCAAAATGTG CAACCGCGTA ATGAAGTTTC TGCTGGTCAT 
    GCTTACACTT GTCTCAGTGA CTCTATGCAA CCTGAACCCT TTTTCCAGAA GGTGGCACAG 
    CCACAAGCCA TACCAGCAGC CTCACCAGTT CTGTCAGCTC AGTCTTCACT GGAAGAGCCT 
    TTTCCACCTC GTTCACGTTC TGTATCACCA GCAAAAAGTA AAGGCAAAAG TTCATTATTG 
    ATTGGGCTCT CTACTGGCCT GTTTGATGCA AACAATCCAA AGATGTTAAG AACATGTTCA 
    CTCCCAGATC TTTCCAAGTT GTACAGAACA CTAATTGACG TTCCCATTGT AGCAGATGTA 
    CAACATCAAG ACAATCTTGA AATAGAGGAT ATGGAAGATG AGCATGTCAA AGAAGGGCAT 
    TCTGATTCTG AAGACATTAT GTTTGGTGAG GCAGATACAG ACTTGCGGGC TCTTCAGGCC 
    TCGATGGAAC AGTTACTTAG GGAGCAGCCT AGTGAGGAAT TTAGTGAAGA GGAGTTGTCT 
    ACCTTAAAGG CAGATACTGC AGCACGCATA ACAAATGGTA CAGAGTTGGA TGATGAGGAT 
    GACAACAACC CCAGCAGTGA AAGTGTACTT AATGAAGAAT GGCAATCAGA CAACAGTGAT 
    GGGGATATTG CCAGTGAATG TGAAGCATAT GACAGTGTCT TCAGCCATTT GGAAGAATTG 
    AGATACAATC TGGAGCGGGA AATAGGCTTT GATAAATTCA TTGAAGTTTA TGATAAAATA 
    AAGGCAATAC ATGAAGATGA AGATGAGAAT ATTGATGTTT GTTCAACAAT AGTTCAGAAT 
    ATTCTGGGAA ATGAATATAA ACATCTGTAT GCCAAGATTC TTCATTTAGT GATGGCAGAT 
    GGAGCCTATC AGGAGGATAA TGATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK1 ( XM_007067095.1 ) cDNA ORF clone, Chelonia mydas(Green sea turtle) -> XP_007067157.1 Chelonia mydas NIMA-related kinase 1 (NEK1), mRNA.
    Price & Availability↑
    CloneID OCh75990
    Clone ID Related Accession (Same CDS sequence) XM_007067095.1
    Accession Version XM_007067095.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1394035200000
    Organism Chelonia mydas(Green sea turtle)
    Product serine/threonine-protein kinase Nek1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006661173.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chelonia mydas Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACAGTG GATGCCTTAG AAAAAGAACC ATGGAGAAAT ATATTAAAGT GCAGAAGATT 
    GGCGAAGGAT CCTTTGGAAA AGCAATTCTG GTCAAAGCTA AAGAAAATAG CAGACAGTAT 
    GTTGTTAAGG AGATAAACAT CTCTAAGATG TCCAATAAAG AGAGAGAAGA ATCAAGGAGA 
    GAAGTTGCTG TATTGGCAAA CATGAAACAT CCAAATATTG TCCTGTATCG GGAATCATTT 
    GAAGAAAATG GCTCTCTCTA TATAGTGATG GATTACTGTG AAGGAGGGGA CCTGTTTAGA 
    CGAATAAATG CTCAGAAAGG CATCTTGTTC TCAGAGGATC AGATAATGGA TTGGTTTGTA 
    CAGATTTGTT TAGCACTAAA ACATGTACAT GACAGAAAAA TTTTGCATCG AGATATAAAG 
    TCACAGAATA TATTTTTAAC CAAAGATGGA ACAATACAAC TGGGAGACTT TGGAATAGCT 
    AGAGTTCTTA ACAGTACTGT AGAGCTGGCT CGCACTTGCA TAGGAACACC ATACTATTTG 
    TCACCTGAAA TCTGTCGGAA CAAGCCTTAC AACAATAAAA GTGACATATG GGCTCTTGGT 
    TGTGTTCTCT ATGAGATGTG CACACTTAAA CATGCATTTG AAGCTGGTAA CATGAAAAAT 
    CTGGTACTGA AGATAATCTC TGGATCTTTT CCTCCTGTTT CTCTGCATTA TTCCTACGAT 
    CTACGTAATC TGCTTTCACA GTTATTTAAA AGGAATCCTA GGGATAGACC ATCAGTCAAC 
    TCCATATTGG AGAAAATCTT TATAGCCAAA CGGGTGGAAA AATTTCTTAC ACCACAGCTT 
    ATTGCAGACG AATTCAGTCA CAAAGTGTTC CACAAGTTTG ACGCCCATGT TGCACCAGTA 
    AAACGACTAG CTCAAGGACA AATCTGTGCT TCAGCTGTGC CAGCTCAGAA AATTACCAAA 
    CCTGCTGCAA AATATGGAGT GCCTTTAATG ATCAAGAAAT CTGGTGATGC ATCTAAAAAA 
    TTTCATGAGA AGAAGCCATT AGCTAAATTT AGACAGGCCC ATCCAACTCC AATGAAGAAA 
    ATGAATCCAG GAGAAGAAAG GAGGAAAATG TTTGAGGAAG CTGCAAAAAA GAGAAGATTT 
    GAATTACTTG AGAAAGAAAA ACGACAAAGA GACCAGGTAG ACTTTCAGAA GGCTGAGCAG 
    ATGAGAAGAC TGGAAAAGGA AAGGATGGAA CGGATAAACA GAGCCAGGGA ACAAGGCTGG 
    AGAAATGTGC TTAGTTCAGG TGGAAGTGGT GAAGTTAAGG CACCCTTTTT TGGCGGTGGA 
    GGAGGTGTTG GGCCTTCCCC TGTGCCTGCC AGAGGACAGT ATGAACACTA CCATGCTATC 
    TTTGACCAGA TGCAACAGCA AAAGGAAAAT ATTAAAGTAG GTGAGGAGAG AGAAACCAGA 
    TGGAGAGCAG AAATACGAAG TCGGGAAGCT GTTGAAAGAG GAGTTCCACC TGGAGTACGT 
    CCAGGATTTC CTAATGGGCC TGATGGTCAT CACCATTTAC CAGATGCTGA TGCAATTAGG 
    AAAAAAATCA AAAGATTGAA GGAGGTGTCT AAACAAGCCA ATGCAAACAG GCAAAGAGGA 
    TGGGTAGCAG CAGAAAGAGC AAAACAAGTT GAAGAATTCT GGCAACGAAA GAGGGAAGCT 
    ATGCAAAACA AAGCTCGTGC TGAAGGACAT ATGGGTACAC TGCAAAACCT GGCAGCTATC 
    TATGGAGGCA GGCCCATTTC TGCAAGAGGA AGGAAATCCA GAAACAAGGA GGAAGAGGAA 
    TACCTGGCAA GATTAAGACA GATCCGACTT CAAAACTTCA ATGAACGCCA GCAGATTAAA 
    GCTAAACTTC GTGGAGAAAA GAATGAAGCT GACGGTTCTG ATGGACAAGA AGGAAGTGAA 
    GAAGCAGAAT TGAGACGCAA AAAAATTGAA GCACTAAAGG CCCAAGCAAA CGCGCGAGCT 
    GCAGTTCTGA AAGAGCAACT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAGAAA 
    GCTTGGGAAG AACATTTGAC AGCAAAAGGG GTAAAGAATC CTATGGTGCC TTTTAATGTG 
    TCAGCAGCTG GGGTTAATCC TGTCCCTGGG CAACAAGAAC CTGGAGGACC TCCTGCCAAA 
    CTACAACTTC CATTGAAGCC CAGTACACCA CTTATATCTA TGACTTCAGC TTTGAAGGAA 
    GTGGGTGTGG ATGAAAATAC AACTGCTATC CAGGAAACCA CAGAGGAAAC GAAGAAGGCA 
    GATCTTGCTG TGCAAAATAA AAGAGAAATA CTAAGAAGAT TGAATCAAAA TCTTAAAACA 
    CAGGACGATG TAAAAGGAAT AAAGGAGCCT AAAGACACCT GTGAGGCAGC TATGGCTGAA 
    GAGAGTAAAG AAGAAGAATA TCCACTTCTA GTAGATCGCA AGAAATGGGA GGTGGCAGCA 
    GCTGAGTTGG TGGTTCCTCT TGCACAGCTA ACTATGGAGG AATCATTGTC GGGGACAGAG 
    AGACAGACCG TGGGGGAGGT AATTAAACTA GATGTTGGTG AGCCCCAGAG AAAAGTTTGG 
    GGCAAAAGCC CTACTGATTC AGTTTTGAAG ATACTGGGGG AAGCAGAGCT ACAGCTTCAA 
    ACTGAATTAT TGGAGGAAAT AATTATCACA AATGATGTTC CTGAAGAAGG TTCTCAGCCC 
    TTAACCGTGA AAGAGAACAG TGAAGTAGAA ATTGCTTGTA TTGGAGAGAA ATCTTCATCT 
    CCAGTTGTTG GTGTCATAGA ATCTGAAAGG ACAATACCAG AGGAGTCACA AACATTTGAT 
    GCCACCCAAG TGCTGTCCAG GCCTGTTGTG CTACATGAGC TGTATGATTT GGAAGCAGAA 
    ATGTTGGAAG AAACTGAAAA AAATAAAAAG TGCAAAAGTG ACCAACTTCT CATCACTGTT 
    CATGATGTGT GGGTTAAAGA AAGGGAAACA ATAACGGAGC ATGAGCCACT GGAAGATACT 
    GCCTGTCAGA AAGATGGTGG TGGTGATGAA GCAGCTTTGG AGAAGGAAAT GCCGCAAAAT 
    GTGCAACCGC GTAATGAAGT TTCTGCTGAC ACTTGTCTCA GTGACTCTAT GCAACCTGAA 
    CCCTTTTTCC AGAAGGTGGC ACAGCCACAA GCCATACCAG CAGCCTCACC AGTTCTGTCA 
    GCTCAGTCTT CACTGGAAGA GCCTTTTCCA CCTCGTTCAC GTTCTGTATC ACCAGCAAAA 
    AGTAAAGGCA AAAGTTCATT ATTGATTGGG CTCTCTACTG GCCTGTTTGA TGCAAACAAT 
    CCAAAGATGT TAAGAACATG TTCACTCCCA GATCTTTCCA AGTTGTACAG AACACTAATT 
    GACGTTCCCA TTGTAGCAGA TGTACAACAT CAAGACAATC TTGAAATAGA GGATATGGAA 
    GATGAGCATG TCAAAGAAGG GCATTCTGAT TCTGAAGACA TTATGTTTGG TGAGGCAGAT 
    ACAGACTTGC GGGCTCTTCA GGCCTCGATG GAACAGTTAC TTAGGGAGCA GCCTAGTGAG 
    GAATTTAGTG AAGAGGAGTT GTCTACCTTA AAGGCAGATA CTGCAGCACG CATAACAAAT 
    GGTACAGAGT TGGATGATGA GGATGACAAC AACCCCAGCA GTGAAAGTGT ACTTAATGAA 
    GAATGGCAAT CAGACAACAG TGATGGGGAT ATTGCCAGTG AATGTGAAGC ATATGACAGT 
    GTCTTCAGCC ATTTGGAAGA ATTGAGATAC AATCTGGAGC GGGAAATAGG CTTTGATAAA 
    TTCATTGAAG TTTATGATAA AATAAAGGCA ATACATGAAG ATGAAGATGA GAATATTGAT 
    GTTTGTTCAA CAATAGTTCA GAATATTCTG GGAAATGAAT ATAAACATCT GTATGCCAAG 
    ATTCTTCATT TAGTGATGGC AGATGGAGCC TATCAGGAGG ATAATGATGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_007067157.1
    CDS51..4004
    Translation

    Target ORF information:

    RefSeq Version XM_007067095.1
    Organism Chelonia mydas(Green sea turtle)
    Definition Chelonia mydas NIMA-related kinase 1 (NEK1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_007067095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAACAGTG GATGCCTTAG AAAAAGAACC ATGGAGAAAT ATATTAAAGT GCAGAAGATT 
    GGCGAAGGAT CCTTTGGAAA AGCAATTCTG GTCAAAGCTA AAGAAAATAG CAGACAGTAT 
    GTTGTTAAGG AGATAAACAT CTCTAAGATG TCCAATAAAG AGAGAGAAGA ATCAAGGAGA 
    GAAGTTGCTG TATTGGCAAA CATGAAACAT CCAAATATTG TCCTGTATCG GGAATCATTT 
    GAAGAAAATG GCTCTCTCTA TATAGTGATG GATTACTGTG AAGGAGGGGA CCTGTTTAGA 
    CGAATAAATG CTCAGAAAGG CATCTTGTTC TCAGAGGATC AGATAATGGA TTGGTTTGTA 
    CAGATTTGTT TAGCACTAAA ACATGTACAT GACAGAAAAA TTTTGCATCG AGATATAAAG 
    TCACAGAATA TATTTTTAAC CAAAGATGGA ACAATACAAC TGGGAGACTT TGGAATAGCT 
    AGAGTTCTTA ACAGTACTGT AGAGCTGGCT CGCACTTGCA TAGGAACACC ATACTATTTG 
    TCACCTGAAA TCTGTCGGAA CAAGCCTTAC AACAATAAAA GTGACATATG GGCTCTTGGT 
    TGTGTTCTCT ATGAGATGTG CACACTTAAA CATGCATTTG AAGCTGGTAA CATGAAAAAT 
    CTGGTACTGA AGATAATCTC TGGATCTTTT CCTCCTGTTT CTCTGCATTA TTCCTACGAT 
    CTACGTAATC TGCTTTCACA GTTATTTAAA AGGAATCCTA GGGATAGACC ATCAGTCAAC 
    TCCATATTGG AGAAAATCTT TATAGCCAAA CGGGTGGAAA AATTTCTTAC ACCACAGCTT 
    ATTGCAGACG AATTCAGTCA CAAAGTGTTC CACAAGTTTG ACGCCCATGT TGCACCAGTA 
    AAACGACTAG CTCAAGGACA AATCTGTGCT TCAGCTGTGC CAGCTCAGAA AATTACCAAA 
    CCTGCTGCAA AATATGGAGT GCCTTTAATG ATCAAGAAAT CTGGTGATGC ATCTAAAAAA 
    TTTCATGAGA AGAAGCCATT AGCTAAATTT AGACAGGCCC ATCCAACTCC AATGAAGAAA 
    ATGAATCCAG GAGAAGAAAG GAGGAAAATG TTTGAGGAAG CTGCAAAAAA GAGAAGATTT 
    GAATTACTTG AGAAAGAAAA ACGACAAAGA GACCAGGTAG ACTTTCAGAA GGCTGAGCAG 
    ATGAGAAGAC TGGAAAAGGA AAGGATGGAA CGGATAAACA GAGCCAGGGA ACAAGGCTGG 
    AGAAATGTGC TTAGTTCAGG TGGAAGTGGT GAAGTTAAGG CACCCTTTTT TGGCGGTGGA 
    GGAGGTGTTG GGCCTTCCCC TGTGCCTGCC AGAGGACAGT ATGAACACTA CCATGCTATC 
    TTTGACCAGA TGCAACAGCA AAAGGAAAAT ATTAAAGTAG GTGAGGAGAG AGAAACCAGA 
    TGGAGAGCAG AAATACGAAG TCGGGAAGCT GTTGAAAGAG GAGTTCCACC TGGAGTACGT 
    CCAGGATTTC CTAATGGGCC TGATGGTCAT CACCATTTAC CAGATGCTGA TGCAATTAGG 
    AAAAAAATCA AAAGATTGAA GGAGGTGTCT AAACAAGCCA ATGCAAACAG GCAAAGAGGA 
    TGGGTAGCAG CAGAAAGAGC AAAACAAGTT GAAGAATTCT GGCAACGAAA GAGGGAAGCT 
    ATGCAAAACA AAGCTCGTGC TGAAGGACAT ATGGGTACAC TGCAAAACCT GGCAGCTATC 
    TATGGAGGCA GGCCCATTTC TGCAAGAGGA AGGAAATCCA GAAACAAGGA GGAAGAGGAA 
    TACCTGGCAA GATTAAGACA GATCCGACTT CAAAACTTCA ATGAACGCCA GCAGATTAAA 
    GCTAAACTTC GTGGAGAAAA GAATGAAGCT GACGGTTCTG ATGGACAAGA AGGAAGTGAA 
    GAAGCAGAAT TGAGACGCAA AAAAATTGAA GCACTAAAGG CCCAAGCAAA CGCGCGAGCT 
    GCAGTTCTGA AAGAGCAACT AGAGCGAAAG AGGAAGGAGG CATATGAAAG AGAGAAGAAA 
    GCTTGGGAAG AACATTTGAC AGCAAAAGGG GTAAAGAATC CTATGGTGCC TTTTAATGTG 
    TCAGCAGCTG GGGTTAATCC TGTCCCTGGG CAACAAGAAC CTGGAGGACC TCCTGCCAAA 
    CTACAACTTC CATTGAAGCC CAGTACACCA CTTATATCTA TGACTTCAGC TTTGAAGGAA 
    GTGGGTGTGG ATGAAAATAC AACTGCTATC CAGGAAACCA CAGAGGAAAC GAAGAAGGCA 
    GATCTTGCTG TGCAAAATAA AAGAGAAATA CTAAGAAGAT TGAATCAAAA TCTTAAAACA 
    CAGGACGATG TAAAAGGAAT AAAGGAGCCT AAAGACACCT GTGAGGCAGC TATGGCTGAA 
    GAGAGTAAAG AAGAAGAATA TCCACTTCTA GTAGATCGCA AGAAATGGGA GGTGGCAGCA 
    GCTGAGTTGG TGGTTCCTCT TGCACAGCTA ACTATGGAGG AATCATTGTC GGGGACAGAG 
    AGACAGACCG TGGGGGAGGT AATTAAACTA GATGTTGGTG AGCCCCAGAG AAAAGTTTGG 
    GGCAAAAGCC CTACTGATTC AGTTTTGAAG ATACTGGGGG AAGCAGAGCT ACAGCTTCAA 
    ACTGAATTAT TGGAGGAAAT AATTATCACA AATGATGTTC CTGAAGAAGG TTCTCAGCCC 
    TTAACCGTGA AAGAGAACAG TGAAGTAGAA ATTGCTTGTA TTGGAGAGAA ATCTTCATCT 
    CCAGTTGTTG GTGTCATAGA ATCTGAAAGG ACAATACCAG AGGAGTCACA AACATTTGAT 
    GCCACCCAAG TGCTGTCCAG GCCTGTTGTG CTACATGAGC TGTATGATTT GGAAGCAGAA 
    ATGTTGGAAG AAACTGAAAA AAATAAAAAG TGCAAAAGTG ACCAACTTCT CATCACTGTT 
    CATGATGTGT GGGTTAAAGA AAGGGAAACA ATAACGGAGC ATGAGCCACT GGAAGATACT 
    GCCTGTCAGA AAGATGGTGG TGGTGATGAA GCAGCTTTGG AGAAGGAAAT GCCGCAAAAT 
    GTGCAACCGC GTAATGAAGT TTCTGCTGAC ACTTGTCTCA GTGACTCTAT GCAACCTGAA 
    CCCTTTTTCC AGAAGGTGGC ACAGCCACAA GCCATACCAG CAGCCTCACC AGTTCTGTCA 
    GCTCAGTCTT CACTGGAAGA GCCTTTTCCA CCTCGTTCAC GTTCTGTATC ACCAGCAAAA 
    AGTAAAGGCA AAAGTTCATT ATTGATTGGG CTCTCTACTG GCCTGTTTGA TGCAAACAAT 
    CCAAAGATGT TAAGAACATG TTCACTCCCA GATCTTTCCA AGTTGTACAG AACACTAATT 
    GACGTTCCCA TTGTAGCAGA TGTACAACAT CAAGACAATC TTGAAATAGA GGATATGGAA 
    GATGAGCATG TCAAAGAAGG GCATTCTGAT TCTGAAGACA TTATGTTTGG TGAGGCAGAT 
    ACAGACTTGC GGGCTCTTCA GGCCTCGATG GAACAGTTAC TTAGGGAGCA GCCTAGTGAG 
    GAATTTAGTG AAGAGGAGTT GTCTACCTTA AAGGCAGATA CTGCAGCACG CATAACAAAT 
    GGTACAGAGT TGGATGATGA GGATGACAAC AACCCCAGCA GTGAAAGTGT ACTTAATGAA 
    GAATGGCAAT CAGACAACAG TGATGGGGAT ATTGCCAGTG AATGTGAAGC ATATGACAGT 
    GTCTTCAGCC ATTTGGAAGA ATTGAGATAC AATCTGGAGC GGGAAATAGG CTTTGATAAA 
    TTCATTGAAG TTTATGATAA AATAAAGGCA ATACATGAAG ATGAAGATGA GAATATTGAT 
    GTTTGTTCAA CAATAGTTCA GAATATTCTG GGAAATGAAT ATAAACATCT GTATGCCAAG 
    ATTCTTCATT TAGTGATGGC AGATGGAGCC TATCAGGAGG ATAATGATGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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