BCKDHB cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCKDHB
Entrez Gene ID	474978
Full Name	branched chain keto acid dehydrogenase E1 subunit beta
General protein information	Preferred Names branched chain keto acid dehydrogenase E1 subunit beta Names 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease)
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005627566.3	XP_005627623.1	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X2
XM_532213.5	XP_532213.2	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
XM_532213.6	XP_532213.2	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
XM_005627565.2	XP_005627622.1	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
XM_005627565.3	XP_005627622.1	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
XM_005627566.2	XP_005627623.1	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007531	XP_308350.2
Oryza sativa (rice)	Os07g0170100	NP_001058989.1
Danio rerio (zebrafish)	bckdhb	NP_001074122.1
Caenorhabditis elegans (roundworm)	tag-173	NP_492149.1
Arabidopsis thaliana (thale cress)	DIN4	NP_187954.1
Canis lupus familiaris (dog)	BCKDHB	XP_005627622.1
Bos taurus (cattle)	BCKDHB	NP_776932.1
Rattus norvegicus (Norway rat)	Bckdhb	NP_062140.1
Xenopus tropicalis (tropical clawed frog)	bckdhb	XP_002936254.2
Magnaporthe oryzae (rice blast fungus)	MGG_06511	XP_003717047.1
Homo sapiens (human)	BCKDHB	NP_898871.1
Gallus gallus (chicken)	BCKDHB	NP_989988.1
Pan troglodytes (chimpanzee)	BCKDHB	XP_001147541.1
Mus musculus (house mouse)	Bckdhb	NP_954665.1
Arabidopsis thaliana (thale cress)	BCDH BETA1	NP_175947.1
Neurospora crassa	NCU03913	XP_957552.1
Drosophila melanogaster (fruit fly)	CG17691	NP_001015354.3

Gene Ontology

The following BCKDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe15519	XM_532213.6 Latest version!	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCe15519	XM_005627565.3 Latest version!	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCe15520	XM_005627566.3 Latest version!	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OCe15519	XM_532213.5	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCe15519	XM_005627565.2	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCe15520	XM_005627566.2	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCKDHB ( XM_532213.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532213.2 Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe15519
Clone ID Related Accession (Same CDS sequence)	XM_532213.5 , XM_532213.6 , XM_005627565.2 , XM_005627565.3
Accession Version	XM_532213.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532213.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532213.2
CDS	61..1224
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_532213.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532213.5

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDHB ( XM_532213.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532213.2 Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe15519
Clone ID Related Accession (Same CDS sequence)	XM_532213.5 , XM_532213.6 , XM_005627565.2 , XM_005627565.3
Accession Version	XM_532213.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532213.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532213.2
CDS	39..1202
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_532213.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532213.6

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDHB ( XM_005627565.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627622.1 Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe15519
Clone ID Related Accession (Same CDS sequence)	XM_532213.5 , XM_532213.6 , XM_005627565.2 , XM_005627565.3
Accession Version	XM_005627565.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627622.1
CDS	61..1224
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_005627565.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627565.2

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDHB ( XM_005627565.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627622.1 Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe15519
Clone ID Related Accession (Same CDS sequence)	XM_532213.5 , XM_532213.6 , XM_005627565.2 , XM_005627565.3
Accession Version	XM_005627565.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627622.1
CDS	39..1202
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_005627565.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627565.3

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGGAAGAAT GCTTCCTGAA TCTAGAGGCT CCTATATCAA GAGTATGTGG GTATGACACA 
CCGTTCCCTC ACATTTTTGA GCCATTCTAC ATCCCAGACA AATGGAAGTG CTATGATGCC 
CTTCGAAAAA TGATCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDHB ( XM_005627566.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627623.1 Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X3, mRNA.

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CloneID	OCe15520
Clone ID Related Accession (Same CDS sequence)	XM_005627566.3 , XM_005627566.2
Accession Version	XM_005627566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627623.1
CDS	41..1069
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQQ

Target ORF information:

RefSeq Version	XM_005627566.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627566.3

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCKDHB ( XM_005627566.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005627623.1 Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe15520
Clone ID Related Accession (Same CDS sequence)	XM_005627566.3 , XM_005627566.2
Accession Version	XM_005627566.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627623.1
CDS	61..1089
Translation	MAAIAATAGRLLRLRAAGAEGQWCRLRGAGLQAASAAGDAAQRRHVAHFTFQPDPEPQEYGQTQKMNLFQAITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDRNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQQ

Target ORF information:

RefSeq Version	XM_005627566.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627566.2

ORF Insert Sequence:

ATGGCGGCGA TTGCGGCGAC TGCGGGCCGG CTCTTGAGGC TGCGTGCGGC CGGGGCAGAA 
GGACAGTGGT GTCGGCTTCG AGGCGCGGGG CTGCAGGCAG CCTCGGCCGC TGGGGATGCG 
GCGCAGAGGC GGCATGTGGC TCACTTCACT TTCCAGCCCG ACCCGGAGCC CCAGGAGTAT 
GGGCAAACTC AGAAAATGAA TCTTTTCCAG GCGATAACAA GTGCCTTGGA TAACTCATTG 
GCTAAAGATC CTACTGCAGT AATATTTGGT GAAGATGTCG CCTTTGGCGG AGTCTTTAGA 
TGTACTGTTG GCTTGCGAGA CAAATATGGA AAAGATAGAG TTTTCAACAC ACCGTTGTGT 
GAACAAGGAA TCGTTGGGTT TGGAATTGGG ATTGCAGTCA CTGGGGCTAC CGCCATTGCA 
GAAATTCAGT TTGCAGATTA TATTTTCCCT GCTTTTGATC AGATTGTTAA TGAAGCTGCC 
AAGTATCGCT ATCGCTCTGG AGATCTTTTT AATTGTGGAA GCCTCACCAT CCGGGCCCCT 
TGGGGCTGTG TGGGCCACGG GGCTCTTTAT CATTCTCAGA GTCCTGAAGC TTTTTTTGCT 
CACTGCCCAG GAATCAAGGT GGTTGTACCC AGAAGCCCTT TCCAGGCCAA GGGACTTCTT 
TTGTCATGCA TAGAGGATAG AAATCCTTGT ATATTTTTTG AACCTAAAAT ACTTTACAGG 
GCAGCAGTTG AACAGGTTCC TGTAGAACCT TACAACATTC CCCTGTCCCA GGCCGAAGTC 
ATTCAGGAAG GGAGCGATGT GACTCTAGTT GCCTGGGGCA CTCAGGTTCA CGTGATCCGA 
GAGGTGGCTT CCATGGCTCA AGAAAAGCTT GGAGTGTCTT GTGAAGTCAT TGATCTGAGG 
ACTATACTAC CCTGGGACGT AGATACAGTT TGCAAGTCTG TGATCAAAAC AGGGCGACTG 
TTAATCAGTC ACGAGGCTCC CTTGACAGGC GGCTTTGCAT CGGAGATCAG CTCTACAGTT 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BCKDHB cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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